Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the prevalence of thyroid dysfunction in a population with borderline iodine deficiency. | we lack information on the influence of borderline iodine deficiency on the occurrence of thyroid dysfunction. iodine deficiency has been reported to facilitate the development of toxic nodular goitre, whereas a high iodine intake may increase the prevalence of autoimmune hypothyroidism. | 1999 | 10469017 |
| [staging of carcinomas of the upper gastrointestinal tract. the current status of diagnostic imaging]. | esophageal carcinoma: ct and endosonography are complementary and the most important imaging modalities at present for staging. after endoscopic and histological diagnosis, ct of the thorax and the abdomen is used. with the proof of local infiltration of a neighbouring organ or in the presence of distant metastasis, palliative therapy can be started. if ct is not conclusive or no local infiltration or distant metastasis is proven, endosonography should be performed. gastric carcinoma: at present ... | 1999 | 10472083 |
| which patients with newly diagnosed prostate cancer need a computed tomography scan of the abdomen and pelvis? an analysis based on 588 patients. | although a computed tomography (ct) scan of the abdomen and pelvis is often recommended as part of the staging evaluation for newly diagnosed prostate cancer, most scans are negative for metastases. we hypothesized that biopsy gleason score, serum prostate-specific antigen (psa) levels, and clinical stage could predict for a positive ct scan and that a low-risk group of patients could be identified in whom ct might be omitted. | 1999 | 10475360 |
| results of proton therapy of uveal melanomas treated in nice. | to present the first results of uveal melanomas treated with the medicyc cyclotron 65 mev proton beam facility in nice, analyzing the factors that affect the cause-specific survival (css), metastatic rate, and reporting the visual outcome. | 1999 | 10477000 |
| [the value of ultrasound in the diagnosis of thyroid diseases in mauritania (171 cases)]. | we investigated thyroid abnormality in mauritania by carrying out a prospective ultrasound study of 171 patients. one hundred and thirty four patients underwent thyroid hormone determination (t3, t4 and tsh) and 123 had complementary plain film x rays of the chest and neck. these ultrasound examinations accounted for 3.8% of all ultrasound examinations performed over the study period. thyroid abnormalities were more frequent in young adults aged 20 to 40 years (67.4%) and in women (83%) in mauri ... | 1999 | 10477408 |
| time course observation of thyroid proliferative lesions and serum levels of related hormones in rats treated with kojic acid after dhpn initiation. | time course changes in thyroid proliferative lesions as well as related hormone levels in the blood of male f344 rats given n-bis(2-hydroxypropyl)nitrosamine (dhpn: 2800 mg/kg body weight, single s.c. injection) as an initiation treatment followed by pulverized basal diet containing 0% (group 2), 2% (group 3) or 4% (group 4) kojic acid (ka) were examined at weeks 1, 2, 4, 8 and 12. as an untreated control group (group 1), rats were given basal diet for 13 weeks and examined in the same manner. s ... | 1999 | 10478329 |
| comparison of immune responses to extracellular domains of mouse and human thyrotropin receptor. | the mouse and human thyrotropin receptors show greater than 87% homology in their amino acid sequences. however, glycosylated extracellular domains of mouse (met-gp) and human (het-gp) thyrotropin receptors showed differences in their ability to react with patient autoantibodies to thyrotropin receptor (tshr). to test for potential differences in their immunogenicity, we immunized balb/c mice with either gel pure non-glycosylated ectodomain of human tshr (etshr ii), or het-gp (het-gp iii), or me ... | 1999 | 10479389 |
| thyroid nodules, thyroid function and dietary iodine in the marshall islands. | thyroid nodules have been found to be common in the population of the marshall islands. this has been attributed to potential exposure of radioiodines from the nuclear weapons tests on bikini and eniwetok between 1946 and 1958. | 1999 | 10480705 |
| interactions of bacteriophage t4-coded primase (gp61) with the t4 replication helicase (gp41) and dna in primosome formation. | one primase (gp61) and six helicase (gp41) subunits interact to form the bacteriophage t4-coded primosome at the dna replication fork. in order to map some of the detailed interactions of the primase within the primosome, we have constructed and characterized variants of the gp61 primase that carry kinase tags at either the n or the c terminus of the polypeptide chain. these tagged gp61 constructs have been probed using several analytical methods. proteolytic digestion and protein kinase protect ... | 1999 | 10480949 |
| c18:1, c18:2 and c18:3 trans and cis fatty acid isomers including conjugated cis delta 9, trans delta 11 linoleic acid (cla) as well as total fat composition of german human milk lipids. | in particular with respect to infant nutrition knowledge of the current contents of trans fatty acids (tfa) and of conjugated linoleic acid (cla in human milk lipids is of interest. after pre-separation by ag-tlc 11 trans-c18:1 isomers could be quantified by gc with a mean total content of 2.40 +/- 0.60 wt% in samples from 40 german women. for the positional isomers t4, t5, t6-8, t9, t10, t11, t12, t13, t14, t15 and t16 contents of 0.02, 0.02, 0.21, 0.37, 0.32, 0.68, 0.23, 0.15, 0.18, 0.09 and 0 ... | 1999 | 10481820 |
| antithyroid drugs inhibit radioiodine-induced increases in thyroid autoantibodies in hyperthyroid graves' disease. | methimazole (mmi) has been reported to affect prognosis in hyperthyroid graves' disease patients treated with radioiodine (131i). in the present study, serum concentrations of thyroxine (t4), triiodothyronine (t3), thyroglobulin (tg), thyrotropin-binding inhibitory immunoglobulin (tbii), thyroglobulin antibody (tgab), and thyroid-peroxidase antibody (tpoab) were measured serially for 1 year in patients with graves' disease after 131i treatment either given alone (group 1, 41 patients) or followe ... | 1999 | 10482369 |
| the oral administration of human thyroglobulin does not affect the incidence of lymphocytic thyroiditis in the biobreeding worcester rat. | oral tolerization with the appropriate antigen(s) to ameliorate autoimmune diseases in humans and in experimentally induced animal models, including experimentally autoimmune thyroiditis in mice, has been reported to be efficacious. spontaneous and iodine induced (0.05% iodine in the drinking water) lymphocytic thyroiditis (lt) occurs in the diabetes mellitus (dm)-prone biobreeding/worcester (bb/wor) rat. the present study was carried out to determine whether the oral administration of human thy ... | 1999 | 10482377 |
| study of the interaction between bacteriophage t4 asia and escherichia coli sigma(70), using the yeast two-hybrid system: neutralization of asia toxicity to e. coli cells by coexpression of a truncated sigma(70) fragment. | the interaction of t4 phage-encoded anti-sigma factor, asia, and escherichia coli sigma(70) was studied by using the yeast two-hybrid system. truncation of sigma(70) to identify the minimum region involved in the interaction showed that the fragment containing amino acid residues proximal to the c terminus (residues 547 to 603) was sufficient for complexing to asia. studies also indicated that some of the truncated c-terminal fragments (residues 493 to 613) had higher affinity for asia as judged ... | 1999 | 10482532 |
| [determination of thyroid hormone]. | measurement of total and free (f) t4 and t3 are widely used as diagnostic methods for thyroid function evaluation. here we review the clinical usefulness of determination of t4, t3, reverse t3(rt3), ft4 and ft3 in patients with autoimmune thyroid disease (aid). autoantibodies against thyroid hormone (thab) are frequently detected in the serum of patients with autoantibodies against thyroglobulin (tgab) in aid. spuriously higher ft4 or ft3 values in recent free thyroid hormone assays [amerlite ma ... | 1999 | 10483253 |
| [serum tsh measurement]. | serum tsh (thyrotropin) measurement is the first-line test to evaluate the thyroid function. recent sensitive serum tsh assays are based on immunometric assays (imas) that use two or more antibodies. electrochemiluminescent immunoassay expanded both the functional sensitivity potential and the range of serum tsh measurement. it is usually combined with serum free t4 measurement to evaluate the thyroid function, but can be used alone when the pituitary function is normal. serum tsh levels do not ... | 1999 | 10483255 |
| [diagnosis and treatment of autoimmune thyroid disease (atd) complicated by pregnancy]. | during pregnancy, distinguishing tbii-negative graves' disease from hcg-related thyrotoxicosis is important. there has been no convincing evidence for any adverse effects of maternal hyper- or hypothyroidism, or thionamides transferred from the mother on fetal organogenesis. in graves' disease, maintaining maternal free t4 within normal range with thionamides may be preferable to fetal euthyroidism when toxemia of pregnancy or glucose intolerance develops, since there is little evidence indicati ... | 1999 | 10483267 |
| hematologic, biochemical, and endocrine effects of dexamethasone on bottlenose dolphins (tursiops truncatus). | two atlantic bottlenose dolphins (tursiops truncatus) were given 0.11 mg/kg dexamethasone p.o., and complete blood count and serum chemistry analyses, including insulin, thyroxine (t4) adrenocorticotrophic hormone (acth), and cortisol level determinations, were performed at 0 hr, 24 hr, 36 hr, 48 hr, 7 days, and 17 days. significant changes included neutrophilia, eosinopenia, lymphopenia, elevated insulin, and depressed acth and cortisol levels within 24 hr of dexamethasone administration. these ... | 1999 | 10484154 |
| loss of pten expression in paraffin-embedded primary prostate cancer correlates with high gleason score and advanced stage. | the tumor suppressor gene pten/mmac-1/tep-1 (referred to hereafter as pten) maps to chromosome 10q23 and encodes a dual specificity phosphatase. the pten protein negatively regulates cell migration and cell survival and induces a g1 cell cycle block via negative regulation of the phosphatidylinositol 3'-kinase/protein kinase b/akt signaling pathway. pten is frequently mutated or deleted in both prostate cancer cell lines and primary prostate cancers. a murine polyclonal antiserum was raised agai ... | 1999 | 10485474 |
| human natural tumor necrosis factor alpha induces multiple endocrine and hematologic disorders in rats. | slc:wistar male rats treated with human natural tumor necrosis factor alpha (hn tnf-alpha, 3 x 10(5) japan reference units/kg intravenously) for 3 months showed histologic vacuolation of basophils in the anterior pituitary, hyperplasia of the thyroidal follicular epithelium, and hyperplasia of the testicular interstitial cells. the vacuolated basophils were immunohistochemically shown to be thyrotrophs. in addition, there were decreases in plasma levels of triiodothyronine (t3), thyroxin (t4), a ... | 1999 | 10485820 |
| padlock oligonucleotides for duplex dna based on sequence-specific triple helix formation. | an oligonucleotide was circularized around double-stranded dna thanks to triple helix formation. short oligonucleotides are known to be able to form dna triple helices by binding into the dna major groove at an oligopurine.oligopyrimidine sequence. after sequence-specific recognition of a double-stranded dna target through triple helix formation, the ends of the triplex-forming oligonucleotide were joined through the action of t4 dna ligase, thus creating a circular dna molecule catenated to the ... | 1999 | 10485872 |
| major capsid proteins of certain vibrio and aeromonas phages are homologous to the equivalent protein, gp23(*), of coliphage t4. | n-terminal amino acid sequences of major capsid proteins (mcps) of three vibriophages (kvp20, kvp40 and nt-1), two aeromonad phages (aeh 1 and 65) and coliphage t4 were compared. all these phages are morphologically similar, belonging to family myoviridae and the vernacular genus name "t4-like phages". a dendrogram constructed from homology data indicated that (i) the three vibriophages were closely related, (ii) the two aeromonad phages were also fairly related and (iii) these five phages were ... | 1999 | 10486118 |
| prognostic role of p53 protein expression in epidermoid carcinoma of the anal canal. | to assess the prognostic significance of p53 protein expression in patients with primary epidermoid carcinoma of the anal canal managed by radiation therapy (xrt), 5-fluorouracil (5-fu), and mitomycin c (mmc). | 1999 | 10487550 |
| pre- and post-radiotherapy computed tomography in laryngeal cancer: imaging-based prediction of local failure. | to determine if pre-radiotherapy (rt) and/or post-radiotherapy computed tomography (ct) can predict local failure in patients with laryngeal carcinoma treated with definitive rt. | 1999 | 10487556 |
| the thyroid hormone receptor-beta gene mutation r383h is associated with isolated central resistance to thyroid hormone. | resistance to thyroid hormone (rth) action is due to mutations in the beta-isoform of the thyroid hormone receptor (tr-beta). rth patients display inappropriate central secretion of trh from the hypothalamus and of tsh from the anterior pituitary despite elevated levels of thyroid hormone (t4 and t3). rth mutations cluster in three hot spots in the c-terminal portion of the tr-beta. most individuals with tr-beta mutations have generalized resistance to thyroid hormone, where most tissues in the ... | 1999 | 10487671 |
| expression and regulation of type ii iodothyronine deiodinase in cultured human skeletal muscle cells. | t4, which is a major secretory product of the thyroid gland, needs to be converted to t3 by iodothyronine deiodinase to exert its biological activity. after the molecular cloning of human type ii iodothyronine deiodinase (dii) complementary dna, dii expression was unexpectedly detected in human skeletal muscle tissue. in the present study, we have identified dii activity and dii messenger ribonucleic acid (mrna) in cultured human skeletal muscle cells and studied the mechanisms involved in the r ... | 1999 | 10487701 |
| isotope dilution-gas chromatography/mass spectrometry and liquid chromatography/electrospray ionization-tandem mass spectrometry for the determination of triiodo-l-thyronine in serum. | isotope dilution-gas chromatography/mass spectrometry (id-gc/ms) and isotope dilution-liquid chromatography/tandem mass spectrometry (id-lc/ms/ms) methods have been developed for an determination of triiodothyronine (t3) in serum and their potential as candidate reference methods investigated. in both methods, (13)c(9)-t3 was used as internal standard. sample pretreatment consisted of deproteinization, extraction and high performance liquid chromatography (hplc) purification. conversion of serum ... | 1999 | 10487939 |
| concomitant radiation therapy and targeted cisplatin chemotherapy for the treatment of advanced pyriform sinus carcinoma: disease control and preservation of organ function. | squamous cell carcinoma of the pyriform sinus is an unfavorable disease which frequently presents in advanced stages. despite aggressive "standard treatment" involving debilitating surgery and postoperative radiation therapy treatments, the survival and functional outcome for pyriform sinus carcinoma remains poor. hence, we reviewed our experience in the management of advanced pyriform sinus carcinoma using "organ preservation" chemoradiation therapy. | 1999 | 10487945 |
| replication slippage of different dna polymerases is inversely related to their strand displacement efficiency. | replication slippage is a particular type of error caused by dna polymerases believed to occur both in bacterial and eukaryotic cells. previous studies have shown that deletion events can occur in escherichia coli by replication slippage between short duplications and that the main e. coli polymerase, dna polymerase iii holoenzyme is prone to such slippage. in this work, we present evidence that the two other dna polymerases of e. coli, dna polymerase i and dna polymerase ii, as well as polymera ... | 1999 | 10488082 |
| substaging of t1 bladder carcinoma based on the depth of invasion as measured by micrometer: a new proposal. | a significant number of t1 bladder carcinoma patients are understaged by transurethral resection of the bladder (turb), indicating a substantial need for more accurate staging. | 1999 | 10491531 |
| megestrol acetate therapy and secondary adrenal suppression. | adrenal suppression has been noted in patients who are receiving medroxyprogesterone acetate (mpa). megestrol acetate (ma) is used to treat patients with advanced breast carcinoma, cachaexia related to acquired immune deficiency syndrome, and disseminated carcinomatosis, and it is believed to have fewer side effects than mpa. the aim of this study was to test for secondary adrenal suppression in patients receiving ma therapy for advanced metastatic cancer. | 1999 | 10491532 |
| epidemiology of histoplasmosis in the french west indies (martinique). | the caribbean islands are presumed to be an endemic zone for histoplasma capsulatum infection, but no epidemiological studies have been done in this area. our purpose was to report the epidemiology of histoplasmosis from 1991 to 1997 in the french west indies (martinique). cases identified from the register of the mycology laboratory were analysed retrospectively. ten cases (9 male and 1 female) were identified; 8 of the patients were infected with hiv (average t4 lymphocyte count in these 8 pat ... | 1999 | 10492755 |
| detection of tamoxifen-dna adducts on laci genes using dna polymerase stop assay. | tamoxifen forms dna adducts in rat liver and causes an increased mutation frequency at the laci genes in the livers of lambda/laci transgenic rats. although an elevated occurrence of endometrial cancer is found in a small proportion of breast cancer patients treated with tamoxifen, there is conflicting evidence on whether or not low levels of dna adducts are formed in humans. | 1999 | 10493022 |
| solution conformation of the (+)-trans-anti-benzo[g]chrysene-da adduct opposite dt in a dna duplex. | the solution structure of the adduct derived from the covalent bonding of the fjord region (+)-(11s, 12r, 13r, 14s) stereoisomer of anti -11,12-dihydroxy-13,14-epoxy-11,12,13, 14-tetrahydrobenzo[g]chrysene, (+)- anti -b[g]cde, to the exocyclic n(6)amino group of the adenine residue da6, (designated (+)- trans-anti -(b[g]c)da6), positioned opposite a thymine residue dt17 in the dna sequence context d(c1-t2-c3-t4-c5-(b[g]c)a6-c7-t8-t9-c10-c11). d(g12-g13-a14-a15-g16-t17-g18-a19-g20++ +-a21-g22) (d ... | 1999 | 10493876 |
| changes in hepatic microcirculation and histomorphology in brain-dead organ donors: an experimental study in rats. | to assess the effect of brain death on hormonal homeostasis, hepatic microcirculation, and histomorphology in organ donors. | 1999 | 10494642 |
| aspecific inflammatory lesion (histiocytosis?) simulating intramedullary astrocytoma. case report. | we report an exceptional case of aspecific inflammatory lesion of the thoracic spinal cord simulating an intramedullary glial tumor. patient history was characterized by progressive spastic paraparesis with urinary incontinence; mr imaging (t4-t5) showed an enhancing intrinsic mass lesion. myelotomy enabled partial resection of grayish astrocytoma-like tissue. only light microscope examination was possible and disclosed aspecific inflammatory tissue composed of eosinophils, lymphocytes and histi ... | 1999 | 10494669 |
| detection of the environmental antiandrogen p,p-dde in cd and long-evans rats using a tier i screening battery and a hershberger assay. | in this report, p,p'-dde, a weak androgen receptor (ar) antagonist, has been examined in a tier i screening battery designed to detect endocrine-active compounds (eacs). the screening battery that was used to examine p,p'-dde was an abbreviated version of a proposed tier i screening battery (cook et al., 1997, regul. toxicol pharmacol. 26, 60-68) that consisted of a 15-day intact male in vivo battery and an in vitro yeast transactivation system (yts). in addition, strain sensitivity differences ... | 1999 | 10496676 |
| detection of thyroid toxicants in a tier i screening battery and alterations in thyroid endpoints over 28 days of exposure. | phenobarbital (pb), a thyroid hormone excretion enhancer, and propylthiouracil (ptu), a thyroid hormone-synthesis inhibitor, have been examined in a tier i screening battery for detecting endocrine-active compounds (eacs). the tier i battery incorporates two short-term in vivo tests (5-day ovariectomized female battery and 15-day intact male battery using sprague-dawley rats) and an in vitro yeast transactivation system (yts). in addition to the tier i battery, thyroid endpoints (serum hormone c ... | 1999 | 10496677 |
| t4 phage beta-glucosyltransferase: substrate binding and proposed catalytic mechanism. | beta-glucosyltransferase (bgt) is a dna-modifying enzyme encoded by bacteriophage t4 which catalyses the transfer of glucose (glc) from uridine diphosphoglucose (udp-glc) to 5-hydroxymethylcytosine (5-hmc) in double-stranded dna. the glucosylation of t4 phage dna is part of a phage dna protection system aimed at host nucleases. we previously reported the first three-dimensional structure of bgt determined from crystals grown in ammonium sulphate containing udp-glc. in this previous structure, we ... | 1999 | 10497034 |
| docetaxel (taxotere) and neoadjuvant chemotherapy for non-small cell lung cancer. | in locally advanced non-small cell lung cancer with ipsilateral (n2) or contralateral (n3) mediastinal node involvement, the presence of micrometastases results in a poor outcome when patients are treated by surgery alone. the prognosis is also bad in inoperable locally advanced disease (t4) treated solely by radiotherapy. compared with surgery or radiotherapy alone, the additional use of cisplatin-based induction chemotherapy has been shown to significantly increase the prospects of long-term s ... | 1999 | 10437748 |
| effects of induced hypothyroidism on weight gains, lactation, and reproductive performance of primiparous brahman cows. | primiparous, spring-calving brahman cows (bw = 425.0 +/- 13.8 kg, body condition score [bcs] = 5.0 +/- .2 units; sem) were used to study the effects of thyroid manipulation on weight gain, milk production, and reproduction. nine cows served as controls. nine cows were induced to become hypothyroid by daily ingestion of 4 mg/kg bw of 6-n-propyl-2-thiouracil (ptu). cows were stratified to treatment 1 d after calving based on season of birth, bw, bcs, calf sex, and calf sire. the treatment period l ... | 1999 | 10438032 |
| binding of the bacteriophage t4 transcriptional activator, mota, to t4 middle promoter dna: evidence for both major and minor groove contacts. | during infection, the bacteriophage t4 transcriptional activator mota, the co-activator asia, and host rna polymerase are needed to transcribe from t4 middle promoters. middle promoters contain a -10 region recognized by the sigma(70)subunit of rna polymerase and a mota box centered at -30 that is bound by mota. we have investigated how the loss or modification of base determinants within the mota box sequence 5'tttgcttta3' (positions -34 to -26 of a middle promoter) affects mota function. gel r ... | 1999 | 10438591 |
| sigma competition: the contest between bacteriophage t4 middle and late transcription. | in bacterial transcription, diverse sigma-family promoter recognition proteins compete for a common rna polymerase core. bacteriophage t4 infection ultimately reduces this competition to a duel between activated viral middle and enhanced late transcription, involving two sigma proteins, two phage-encoded activator proteins and two phage-specific co-activators. this competition has been analyzed in vitro, and the relative abundances in t4-infected escherichia coli of the participating proteins ha ... | 1999 | 10438620 |
| mandibulofacial adaptations in a juvenile animal model of temporomandibular joint arthritis. | juvenile rheumatoid arthritis (jra) is a chronic systemic disease of childhood that affects synovial joints including the temporomandibular joint (tmj). individuals with jra of the tmj frequently show aberrations in mandibulofacial development. since the basis for these developmental perturbations is poorly understood, they remain a perplexing clinical problem to manage. to begin dissecting the mechanisms for altered craniofacial development in jra of the tmj, we characterized the gross morpholo ... | 1999 | 10439030 |
| anterior transsternal approach to the upper thoracic spine. | cervicothoracic junction and upper thoracic spine down to t4 can be reached through anterior approach via sternotomy. transsternal approach is the best route to gain access to lesions localized within vertebral bodies of the upper thoracic spine allowing for their resection, interbody fusion and replacement with bone cement. consecutive modifications of transsternal approach evolved towards less extensive osteotomy from full median sternotomy, through manubriotomy with clavicle resection to part ... | 1999 | 10439103 |
| does postoperative irradiation play a role in the adjuvant therapy of stage t4 colon cancer? | this study analyzes the long-term outcome of patients with stage t4 colon cancer who receive postoperative irradiation. the purpose of the study is to define the potential role of this modality with current systemic therapies. | 1999 | 10439171 |
| [hormonal metabolic status in breast cancer patients after conservative surgery: comparison with known prognostic criteria]. | body weight, body mass index, body fat, lean body mass, blood-glucose, cholesterol, hdl-cholesterol, triglyceride, beta-lipoproteins, insulin, gonadotropin, estradiol, testosterone, shbg, t3, t4 and tsh levels as well as estradiol and progesterone receptor levels in excised tumor were studied in 40 patients with breast cancer prior to conservative treatment. said anthropometric, metabolic and hormonal parameters were compared with the index of lymphocytic infiltration of tumor selected as a prog ... | 1999 | 10443228 |
| outcome of a baby born from a mother with acquired juvenile hypothyroidism having undetectable thyroid hormone concentrations. | we report a baby born from a mother with strongly positive thyroid stimulation blocking antibody (tsbab) and nearly undetectable t4 level. this case is a unique model of nearly complete absence of thyroid hormones during fetal and early neonatal life in humans. the infant girl was born by cesarean section, because of fetal bradycardia, after 41 weeks gestation and received mechanical ventilation for 3 days. the tsh level was more than 120 microu/ml in the neonatal thyroid screening. at age 17 da ... | 1999 | 10443651 |
| in vivo and in vitro regulation of thyroid leukemia inhibitory factor (lif): marker of hypothyroidism. | several cytokines regulate thyroid function and may be involved in the pathogenesis of thyroid disorders, including euthyroid sick syndrome. leukemia inhibitory factor (lif), a neuroimmune pleiotropic cytokine, was measured to assess its role in hypothalamic-pituitary-thyroid function. mean circulating serum lif levels in 10 hypothyroid patients [tsh, 23+/-0.5 miu/l (mean+/-sem); free t4, 0.77+/-0.1 ng/dl] was 0.29+/-0.04 ng/ml, 145% higher (p < 0.04) than in 20 normal subjects (lif, 0.20+/-0.02 ... | 1999 | 10443695 |
| thyroid hormone alpha1 and beta1 receptor mrna are downregulated by amiodarone in mouse myocardium. | amiodarone, a powerful antiarrhythmic drug, may exert its effect by antagonism of the thyroid hormone, probably at the receptor level. the aim of this study was to investigate whether amiodarone affects the levels of thyroid hormone receptor (tr) messenger rna (mrna) subtypes in mouse hearts. mice were treated with 10, 25, and 50 mg/kg body weight (bw) amiodarone or vehicle (propyleneglycol) intraperitoneally, daily for 14 days. the heart rate dose-dependently decreased in the 25 mg/kg bw (p < 0 ... | 1999 | 10445678 |
| effects of phenobarbital, pregnenolone-16alpha-carbonitrile, and propylthiouracil on thyroid follicular cell proliferation. | reduced thyroid hormone concentrations (t4 and/or t3) and increased thyroid-stimulating hormone (tsh) have been proposed to mediate the thyroid tumor promoting effects of hepatic microsomal enzyme inducers (mei) and antithyroid drugs. tsh is known to stimulate thyroid gland function and growth, as well as neoplasia. thyroid weight has been used as an indicator of thyroid gland growth in mei studies, but little is known about the effects of these inducers on thyroid cell proliferation. therefore, ... | 1999 | 10445752 |
| structural characterization of rna polymerase ii complexes arrested by a cyclobutane pyrimidine dimer in the transcribed strand of template dna. | we have characterized the properties of immunopurified transcription complexes arrested at a specifically located cyclobutane pyrimidine dimer (cpd) using enzymatic probes and an in vitro transcription system with purified rna polymerase ii (rnap ii) and initiation factors. to help understand how rnap ii distinguishes between a natural impediment and a lesion in the dna to initiate a repair event, we have compared the conformation of rnap ii complexes arrested at a cpd with complexes arrested at ... | 1999 | 10446184 |
| molecular characterization of a human dna kinase. | human polydeoxyribonucleotide kinase is an enzyme that has the capacity to phosphorylate dna at 5'-hydroxyl termini and dephosphorylate 3'-phosphate termini and, therefore, can be considered a putative dna repair enzyme. the enzyme was purified from hela cells. amino acid sequence was obtained for several tryptic fragments by mass spectrometry. the sequences were matched through the dbest data base with an incomplete human cdna clone, which was used as a probe to retrieve the 5'-end of the cdna ... | 1999 | 10446193 |
| enhanced amsacrine-induced mutagenesis in plateau-phase chinese hamster ovary cells, with targeting of +1 frameshifts to free 3' ends of topoisomerase ii cleavable complexes. | previous work showed that the dna double-strand cleaving agents bleomycin and neocarzinostatin were more mutagenic in plateau-phase than in log-phase cells. to determine whether topoisomerase ii poisons that produce double-strand breaks by trapping of cleavable complexes would, likewise, induce mutations specific to plateau-phase cells, aprt mutations induced by amsacrine in both log-phase and plateau-phase cho cells were analyzed. the maximum aprt mutant frequencies obtained were 7 x 10(-6) aft ... | 1999 | 10446982 |
| what happens to the normal thyroid during pregnancy? | hormonal changes and metabolic demands during pregnancy result in profound alterations in the biochemical parameters of thyroid function. for the thyroidal economy, the main events occurring during pregnancy are: a marked increase in serum thyroxine-binding globulin levels; a marginal decrease in free hormone concentrations (in iodine-sufficient conditions) that is significantly amplified when there is iodine restriction or overt iodine deficiency; a frequent trend toward a slight increase in ba ... | 1999 | 10447005 |
| human chorionic gonadotropin and the thyroid: hyperemesis gravidarum and trophoblastic tumors. | there is abundant evidence that human chorionic gonadotropin (hcg) is a weak thyrotropin (tsh) agonist. in frtl-5 rat thyroid cells, hcg increases cyclic adenosine monophosphate (camp), iodide transport, and cell growth. hcg has thyroid-stimulating activity in bioassays in mice and in clinical studies in man. in cultured cells transfected with the human tsh receptor, hcg increases generation of camp. molecular variants of hcg with increased thyrotropic potency include basic molecules with reduce ... | 1999 | 10447009 |
| thyroid hormone and intellectual development: a clinician's view. | cretinism, which is characterized by marked intellectual impairment and is associated with severe iodine deficiency, has been appreciated for many years. advances in the knowledge of thyroid physiology and its changes in normal pregnancy have now led to the realization of the potential importance of minor changes in maternal thyroxine (t4) concentrations in relation to brain maturation and development. mild and subclinical neuropsychomotor deficits have been observed in neonates both in mildly i ... | 1999 | 10447010 |
| maternal hypothyroidism: recognition and management. | women with compensated early thyroid failure, or those from areas of reduced iodine intake, may first be found to be hypothyroid during pregnancy. in women with previously diagnosed hypothyroidism already on thyroxine (t4) replacement therapy, pregnancy is often associated with an increased dose requirement. the mechanism producing this increased requirement is not known, but it is likely to be the result of a number of factors that may differ depending on the stage of pregnancy. an increased t4 ... | 1999 | 10447011 |
| hypothyroxinemia in premature infants: is thyroxine treatment necessary? | the increased survival of very low birth weight (vlbw) premature infants has been associated with an increased prevalence of transient disorders of thyroid function during the early weeks of life. all vlbw infants have relatively low, and gestation age-dependent, thyroxine-binding globulin (tbg) concentrations associated with variably low total thyroxine (t4) concentrations. these infants also have a high prevalence (30%-60%) of nonthyroidal illnesses that impact thyroid function, including tota ... | 1999 | 10447019 |
| congenital hypothyroidism: etiologies, diagnosis, and management. | congenital hypothyroidism is a common preventable cause of mental retardation. the overall incidence is approximately 1:4000; females are affected about twice as often as males. approximately 85% of cases are sporadic, while 15% are hereditary. the most common sporadic etiology is thyroid dysgenesis, with ectopic glands more common than aplasia or hypoplasia. while the pathogenesis of dysgenesis is largely unknown, some cases are now discovered to be the result of mutations in the transcription ... | 1999 | 10447022 |
| congenital hypothyroidism: long-term outcome. | although mental retardation associated with congenital hypothyroidism (ch) is prevented by newborn screening and early treatment, affected children still undergo a brief period of thyroid hormone deficiency reflecting etiology of thyroid disease, illness severity, and treatment factors. because thyroid hormone is essential for normal brain development and because some processes require thyroid hormone in the period when thyroid hormone was lacking, children with ch treated early may still have s ... | 1999 | 10447023 |
| the catalytic group-i introns of the psba gene of chlamydomonas reinhardtii : core structures, orfs and evolutionary implications. | the sequences and predicted secondary structures of the four catalytic group-i introns in the psba gene of chlamydomonas reinhardtii, cr.psba-1-cr.psba-4, have been determined. cr.psba-1 and cr.psba-4 are subgroup-ia1 introns and have similar secondary structures, except at the 3' end where cr.psba-1 contains a large inverted-repeat domain. cr.psba-4 is closely related to intron 1 of the chlamydomonas moewusii psba gene, with which it shares the same location, high nucleotide identity in the cor ... | 1999 | 10447597 |
| double-blind, randomised, multicentre endocrine trial comparing two letrozole doses, in postmenopausal breast cancer patients. | letrozole is an orally competitive aromatase inhibitor. this double-blind, randomised, multicentre trial was carried out to evaluate the endocrine effects of two doses of letrozole, 0.5 mg versus 2.5 mg orally daily, in postmenopausal advanced breast cancer patients progressing after tamoxifen. the pharmacokinetics of letrozole was also assessed. 46 patients entered the trial, 22 on letrozole 0.5 mg and 24 on 2.5 mg. a significant suppression of oestrone and oestradiol levels was achieved by bot ... | 1999 | 10448261 |
| the examiner's learning effect and its influence on the quality of endoscopic ultrasonography in carcinoma of the esophagus and gastric cardia. | the preoperative diagnosis of tumors of the esophagus and the gastric cardia is an important element in their stage-oriented therapy. the goal of the present study was to evaluate the accuracy of endosonographic ultrasound (eus) and to test its usefulness in tumor staging and the assessment of operability. | 1999 | 10449847 |
| dietary fat and triiodothyronine (t3) interactions in the broiler chicken. | male, indian river, broiler chickens growing from 7 to 28 d were fed on diets containing 180 g crude protein, 300, 600 or 1200 kcal fat calories and 0 or 1 mg t3/kg diet. birds were bled and sacrificed at d 28, 29 and 30. measurements taken at these intervals included in vitro lipogenesis (ivl), growth and feed consumption and hepatic enzyme activities. plasma metabolites as well as thyroid hormones were also determined. dietary fat levels did not influence growth or feed intake. in contrast, t3 ... | 1999 | 10450536 |
| spinal block levels and cardiovascular changes during post-cesarean transport. | transport after surgery under spinal anesthesia is associated with cardiovascular changes. the extensively vasodilated patient may be unable to compensate for postural blood flow redistribution. this observational study investigated pre- and post-surgery sensory levels as well as hemodynamic changes during the postoperative transfer period. | 1999 | 10451132 |
| solution structure of the (+)-cis-anti-benzo[a]pyrene-da ([bp]da) adduct opposite dt in a dna duplex. | minor adducts, derived from the covalent binding of anti-benzo[a]pyrene-7,8-dihydroxy-9,10-epoxide to cellular dna, may play an important role in generating mutations and initiating cancer. we have applied a combined nmr-computational approach including intensity based refinement to determine the solution structure of the minor (+)-cis-anti-[bp]da adduct positioned opposite dt in the d(c1-t2-c3-t4-c5-[bp]a6-c7-t8-t9-c10-c11). (d(g12-g13-a14-a15-g16-t17-g18-a19-g20+ ++-a21-g22) 11-mer duplex. the ... | 1999 | 10451380 |
| metamorphosis in the summer flounder, paralichthys dentatus: thyroidal status influences salinity tolerance. | metamorphosis in the summer flounder (paralichthys dentatus) is controlled by thyroid hormones (th) and takes place as the larvae move from a salinity of about 35 parts per thousand (ppt) in the ocean to salinity ranging from 0-35 ppt in estuaries. historically, the role of th in juvenile and adult teleost osmoregulation has been ambiguous, and it is not known if th influences larval teleost osmoregulatory development. this study addresses the influence of thyroxine (t4) on the development of to ... | 1999 | 10451419 |
| [zinc inhibits the in vitro conversion of thyroxine to triiodothyronine in brown adipose issue]. | the effect of in vitro addition of zinc sulphate on t4 deiodination in brown adipose tissue (bat) of rats exposed to 4 degrees c or 22 degrees c temperature during 24 h, was studied. animals were killed by cervical dislocation and bat was immediately removed and homogenized in sucrose buffer (320 mm) containing hepes (10 mm) ph 7.4. the preparation was centrifuged at 4 degrees c during 10 min. aliquots were separated adding 50, 100 microm, 1 o 5 mm zinc sulphate plus 0, 5, 10 or 25 mm dithiothre ... | 1999 | 10451566 |
| myocardial 123i-mibg kinetics in acutely hypothyroid patients with differentiated thyroid carcinoma. | marked changes in cardiac function have been noted in patients with hyperthyroidism or hypothyroidism due both to changes in sympathetic system function and to biochemical modifications of myocardial tissue. metaiodobenzylguanidine (mibg), an analogue of norepinephrine, can be used to evaluate myocardial sympathetic tone. here, we report myocardial 123i-mibg kinetics in patients with differentiated thyroid carcinoma undergoing acute hypothyroidism followed by hormonal replacement as part of thei ... | 1999 | 10451880 |
| [acidosis, hypoxia and hypotension in abdominal chemotherapy with blockade of arterial and venous circulation (stop flow)]. | a study was performed of the perioperative systemic effects of a recent zoned chemotherapy technique administered in conditions of extreme acidosis, hypoxia and modern hypotension. | 1999 | 10206036 |
| role of endosonography in the evaluation of gastric malignancies. | the aims of this study were to stage gastric malignancies and differentiate between carcinomas and lymphomas. twenty-two patients were evaluated with endosonography. tumoral infiltration depth (t stage) and presence of metastatic lymph nodes (n stage) were investigated. five percent of the cases were staged as t2, 36% as t3, and 59% as t4. according to the endosonographic examination, 14% were no, 68% were n1, and 18% were n2 malignancies. when compared with results of surgery and histopathologi ... | 1999 | 10206216 |
| cortical distribution of bereitschaftspotential and negative slope potential preceding mouth-opening movements in humans. | cortical potentials associated with the voluntary movement of various body parts are known as movement-related cortical potentials, which allow evaluation of the cortical efferent function and other higher functions controlling voluntary movement. the cortical potentials have three main components: the bereitschaftspotential (or 'readiness potential'), the negative slope and the motor potential. here, the cortical potentials preceding mouth-opening movements were recorded to investigate which of ... | 1999 | 10206336 |
| changes in thyroid function tests during short-term salsalate use. | although long-term administration of salsalate depresses blood levels of both total thyroxine (t4) and total triiodothyronine (t3) and at least transiently decreases serum thyrotropin (tsh), changes in thyroid function tests have not been fully characterized during its short-term use. it is also unclear if the observed changes are solely the result of decreased hormone binding to carrier proteins or if reduced hepatic 5'-monodeiodinase activity is important. blood was sampled at baseline (day 0) ... | 1999 | 10206445 |
| changes in gastric intramucosal ph following mesenteric traction in patients undergoing pancreas surgery. | during major abdominal surgery, mesenteric traction (mt) may result in hemodynamic instability mainly due to endogenous prostacyclin release. gastric intramucosal ph (phi) and pico2 are indicators of splanchnic tissue perfusion with a predictive value for the postoperative outcome. we investigated the influence of mt on gastric phi and on postoperative outcome in patients undergoing pancreas surgery. | 1999 | 10207237 |
| anterior craniofacial resection for malignant ethmoid tumors--a series of 91 patients. | anterior craniofacial resection is now recognized as the best treatment for ethmoid tumors involving the cribriform plate with or without invasion of anterior cranial fossa. | 1999 | 10208659 |
| construction of 4-thiouridine site-specifically substituted rnas for cross-linking studies. | availability of 4-thiouridine (4-thiou)-containing rnas is the prerequisite for 4-thiou site-specific cross-linking studies. this paper presents a method for constructing such rnas. a 5'- and a 3'-rna are synthesized via phage rna polymerase transcription and/or rnase h site-specific cleavage directed by 2'-o-methyl-rna-dna chimeras. these two half-rnas in combination correspond to the sequence of full-length rna, with a single nucleotide gap at the junction that will be filled in with a 4-thiou ... | 1999 | 10208812 |
| pedicle and transverse process screws of the upper thoracic spine. biomechanical comparison of loads to failure. | an in vitro biomechanical load-to-failure test. | 1999 | 10209793 |
| [breast cancer in tunisia: clinical and epidemiological study]. | this study has been realized to determine epidemiological profile and clinicopathologic aspect of breast cancer in tunisia. we have counted and analyzed all cancers of the breast diagnosed in tunisia with proof pathologic or to defect cytologic of malignancy, between first january 1994 and 31 december 1994. in the course of this year, 689 new cases of mammary cancers have been diagnosed at the women. the average patient age was 50.0 years, the incidence standardized on the age of the cancer of t ... | 1999 | 10210765 |
| dermal mucinosis and musculoskeletal symptoms simulating polymyositis as a presenting sign of hypothyroidism. | a case of dermal mucinosis and musculoskeletal symptoms simulating polymyositis as a presenting sign of hypothyroidism is presented. the patient presented muscle weakness and edema of the face including the eyelids. laboratory examination revealed elevated creatinine phosphokinase, decreased free-t4, decreased free-t3, elevated tsh, positive anti-microsome antibody and positive anti-tsh receptor antibody. the skin biopsy specimen revealed swelling of the collagen bundles with the bundles splitti ... | 1999 | 10210791 |
| sequence-specific cleavage by bacteriophage t4 endonuclease ii in vitro. | the 136 codon (408 bp) dena gene encoding endonuclease ii (endoll) of bacteriophage t4 was unambiguously identified through sequencing and subsequent cloning. endoll prepared from cloned dna through coupled in vitro transcription-translation nicked and cut dna in vitro in a sequence-specific manner. in vitro (and in vivo), the bottom strand was nicked between the first and second base pair to the right of a top-strand ccgc motif shared by favoured in vitro and in vivo cleavage sites; top-strand ... | 1999 | 10200960 |
| effects of three principal constituents in chewing gum on electroencephalographic activity. | the effects of three principal constituents (sugar, spearmint, and gumbase) in chewing gum on eeg findings in 20 normal subjects were investigated. after they chewed the gumbase, theta activity decreased significantly at fp2, o1, t3, t4, f4, p3, and pz; alpha activity increased significantly at o1 and decreased significantly at fpl, and beta activity increased significantly at fpl, fp2, o1, t3, t4, p3 and pz. after they inhaled the spearmint, alpha activity decreased significantly at 02, f3, p4, ... | 1999 | 10201279 |
| prognostic factors affecting survival in patients with colorectal carcinomatosis. | this study evaluates prognostic factors that may influence survival in patients who present with carcinomatosis from colorectal cancer. patients may present with carcinomatosis as the pattern of metastases at the initial diagnosis of colorectal cancer. little is known about the natural history of carcinomatosis and the prognostic factors affecting outcome. all patients treated at roswell park cancer institute from 1988 to 1994 who presented with carcinomatosis at the initial diagnosis of colorec ... | 1999 | 10225003 |
| predicting the local outcome of glottic squamous cell carcinoma after definitive radiation therapy: value of computed tomography-determined tumour parameters. | the t-classification has shortcomings in the prediction of local outcome of glottic squamous cell carcinoma (scc) treated by definitive radiation therapy. in this regard, the value of several ct-derived tumour parameters as predictors of local outcome was investigated. | 1999 | 10225556 |
| comparison of comparative genomic hybridization, fluorescence in situ hybridization and flow cytometry in urinary bladder cancer. | comparative genomic hybridization (cgh) was applied to screen the genetic events in six invasive urinary bladder cancers. these cases were also studied by flow cytometry (fcm) and fluorescence in situ hybridization (fish). four samples showed partial gain on chromosome 8, with the common region involved was on 8q23-qter. full or partial deletion on chromosome 2 and 17p in addition to gain on 20q was found in two cases. interestingly one diploid tumor with low mitotic index, stage and grade showe ... | 1999 | 10226518 |
| prognosis of patients with t4 prostate carcinoma. | the clinical course of patients with t4 prostate carcinoma was examined in order to consider whether there are alternative treatment options besides androgen ablation therapy. | 1999 | 10226837 |
| purification of a polynucleotide kinase from calf thymus, comparison of its 3'-phosphatase domain with t4 polynucleotide kinase, and investigation of its effect on dna replication in vitro. | mammalian polynucleotide kinases (pnks) carry out 5'-phosphorylation of nucleic acids. although the cellular function(s) of these enzymes remain to be delineated, important suggestions have included a role in dna repair and, more recently, in dna replication. like t4 pnk, some preparations of mammalian pnks have been reported to have an associated 3'-phosphatase activity. previously, we have identified in calf thymus glands an apparently novel pnk with a neutral to alkaline ph optimum that lacke ... | 1999 | 10227382 |
| mixed agonistic-antagonistic cytokine response in whole blood from patients undergoing abdominal aortic aneurysm repair. | to characterize the impact of abdominal aortic aneurysm repair (aaar) on spontaneous as well as lipopolysaccharide (lps)-induced gene expression of pro- and anti-inflammatory cytokines. | 1999 | 10229162 |
| the distribution, determinants, and clinical correlates of vertebral osteophytosis: a population based survey. | vertebral osteophytes are a characteristic feature of intervertebral disc degeneration. there are, however, few population data concerning the occurrence of and clinico-biological correlates of vertebral osteophytes in both the dorsal and lumbar spine. our purpose was to determine the frequency and distribution of anterior osteophytes in the thoracic and lumbar spine, and their relationship with both various putative risk factors, including physical activity and obesity, and self-reported back p ... | 1999 | 10229405 |
| segmental roentgenographic analysis of vertebral inclination on sagittal plane in asymptomatic versus chronic low back pain patients. | two homogenous groups of 120 volunteers and 120 low back pain (lbp) patients, age range 20-79 years, underwent a prospective roentgenographic segmental vertebral analysis of the thoracic and lumbar spine to compare several roentgenographic parameters useful for planning spine surgery. the following roentgenographic parameters were measured: thoracic kyphosis, lumbar lordosis, sacral inclination, distal lordosis (l4-s1), inclination of each vertebra from t4-s1, and relative vertebral inclination ... | 1999 | 10229527 |
| role of the bcl-2 gene family in prostate cancer progression and its implications for therapeutic intervention. | prostate cancer (pc) is an escalating health burden in the western world. a large number of patients still present with extraprostatic (i.e., t3/t4, n0, m0/m1 or any t category and m1 disease or involved lymph nodes) and therefore incurable disease. since the work of huggins in 1940, there have been no major therapeutic advances and androgen ablation remains the best treatment option for extraprostatic androgen-responsive pc. eighty to ninety percent of pc patients respond well to this form of t ... | 1999 | 10229706 |
| school attainments in children with congenital hypothyroidism detected by neonatal screening and treated early in life. | evaluation of school attainments in children with congenital hypothyroidism (ch) detected by neonatal screening and treated early in life. | 1999 | 10229905 |
| identification of thyroid hormone residues on serum thyroglobulin: a clue to the source of circulating thyroglobulin in thyroid diseases. | thyroglobulin (tg) present in the serum of normal individuals and patients with thyroid disorders could be partly newly synthesized non-iodinated tg and partly tg containing iodine and hormone residues originating from the lumen of thyroid follicles. with the aim of examining the contribution of the latter source of tg to the elevation of serum tg concentration in thyroid pathophysiological situations, we devised a procedure to identify thyroxine (t4) and tri-iodothyronine (t3) residues on tg fr ... | 1999 | 10229914 |
| a new modification of the 32p-post-labeling method to recover iq-dna adducts as mononucleotides. | to obtain accurate estimates of dna adduct levels yielded by genotoxic compounds, it is essential to completely digest adducted nucleotides to mononucleotides. we previously developed a suitable method, called modified method i, to obtain dna adducts of heterocyclic amines as 32p-labeled-mononucleoside adduct 5'-phosphate forms, by use of nuclease p1 (np1) and phosphodiesterase i (pdei) to digest adducted oligonucleotides. in this study, we applied method i to 2-amino-3-methylimidazo[4,5-f]quino ... | 1999 | 10229928 |
| an examination of mood changes and performance in a professional basketball team. | the purpose of this study was to examine the relationship between the profile of mood states (poms) and performance in a professional basketball team. | 1999 | 10230174 |
| possible effect of calcitonin deficiency on bone mass after subtotal thyroidectomy. | bone mass is purportedly reduced by an excess of endogenous or exogenous thyroid hormone or perhaps by calcitonin deficiency. patients who have undergone thyroidectomy could be subject to all of these effects. in the present study we tried to demonstrate, whether lack of calcitonin following thyroidectomy has a significant influence on bone density. we measured thyroid hormone levels, tsh and calcitonin and assessed the bone mass in the hip and lumbar spine of 55 patients (32 f, 23 m), who had u ... | 1999 | 10230474 |
| metabolic disturbances in male broilers of different strains. 2. relationship between the thyroid and somatotropic axes with growth rate and mortality. | seven male broiler strains (arbor acres, avian farms, cobb-500, hubbard-peterson, isa, naked neck, and ross) were compared for their growth rate, feed efficiency, and mortality due to sudden death and ascites. in addition, weekly plasma levels of thyroid hormones [3,3',5-triiodothyronine (t3) thyroxine (t4), t3: t4 ratio, growth hormone (gh), and insulin-like growth factor-i (igf-i)] were determined. the highly productive, commercial strains were very similar in their endocrine profiles but diff ... | 1999 | 10230903 |
| predictive value of flow cytometry for metastatic potential in breast cancer. | breast cancer is the leading malignancy in women in the united states. tumor size and nodal metastases have been the most important predictors of patient outcome and determinants of treatment, but have also been used to predict metastatic potential. this study was undertaken to ascertain the predictive value of flow cytometry for lymph node or systemic metastases. from 1994 through 1997, surgical specimens from 106 women who underwent treatment for invasive breast cancer were reviewed. epidemiol ... | 1999 | 10231212 |
| characterization of a self-splicing mini-intein and its conversion into autocatalytic n- and c-terminal cleavage elements: facile production of protein building blocks for protein ligation. | the determinants governing the self-catalyzed splicing and cleavage events by a mini-intein of 154 amino acids, derived from the dnab gene of synechocystis sp. were investigated. the residues at the splice junctions have a profound effect on splicing and peptide bond cleavage at either the n- or c-terminus of the intein. mutation of the native gly residue preceding the intein blocked splicing and cleavage at the n-terminal splice junction, while substitution of the intein c-terminal asn154 resul ... | 1999 | 10231563 |
| co-expression of gene 31 and 23 products of bacteriophage t4. | folding of the major capsid protein of bacteriophage t4 encoded by gene 23 is aided by escherichia coli groel chaperonin and phage co-chaperonin gp31. in the absence of gene product (gp) 31, aggregates of recombinant gp23 accumulate in the cell similar to inclusion bodies. these aggregates can be solubilized with 6 m urea. however, the protein cannot form regular structures in solution. a system of co-expression of gp31 and gp23 under the control of phage t7 promoter in e. coli cells has been co ... | 1999 | 10231589 |
| lung morphometry and collagen and elastin content: changes during normal development and after prenatal hormone exposure in sheep. | this study examined whether the improvement in lung function after prenatal hormone exposure coincided with changes in lung morphometry or in collagen and elastin content. fetal lambs received a single intramuscular injection of betamethasone (0.5 mg/kg) plus l-thyroxine (t4) (15 micrograms/kg) or vehicle control 48 h before delivery at 121, 128, or 135 d gestational age (d 121, d 128, d 135, term = 150 d). t4 was administered in conjunction with betamethasone in an attempt to enhance the matura ... | 1999 | 10231854 |