Publications

the cooperativity of burst phase reactions explored.	the denaturant-dependence of the major, observable relaxation rates for folding (kobs) of ribonuclease hi from escherichia coli (rnase h) and phage t4 lysozyme (t4l) reveal that, for both proteins, folding begins with the rapid and transient accumulation of intermediate species in a "burst phase" which precedes the rate-limiting formation of the native state; this is evidenced by a "rollover" in the folding limb of the rate profiles (kobs versus denaturant, or chevron plot). these rate profiles ...	1999	10547295
treatment of maxillary sinus carcinoma: a comparison of the 1997 and 1977 american joint committee on cancer staging systems.	this study was conducted to assess the effectiveness of the 1997 american joint committee on cancer (ajcc) staging system to predict survival and local control of patients with maxillary sinus carcinoma and to identify significant factors for overall survival, local control, and distant metastases in patients with these tumors.	1999	10547542
surgical and multimodal approaches to cancer of the oesophagus: state of the art.	this review article aims to discuss the modalities of oesophageal resection, to define the categories of patients who are most likely to benefit from oesophagectomy with extensive lymph node clearance, and to analyse the eventual contribution of nonsurgical neo-adjuvant or adjuvant therapies to improving long-term survival rates achieved by surgery alone. both the review of the literature devoted to potentially curative treatment of oesophageal cancer and the authors' own experience indicate tha ...	1999	10547892
successful weaning from cardiopulmonary bypass with central venous prostaglandin e1 and left atrial norepinephrine infusion in patients with acute pulmonary hypertension.	postoperative pulmonary hypertension increases the mortality risk in cardiac surgery. we have used central venous prostaglandin e1 (pge1) and left atrial norepinephrine (ne) infusion to wean from cardiopulmonary bypass (cpb) patients with refractory postoperative pulmonary hypertension.	1999	10548203
[neuromuscular recovery following mivacurium is predictable in patients with severe systemic disease prognoses].	fast recovery from mivacurium-induced neuromuscular blockade is impaired in patients with decreased plasma cholinesterase activity which is often associated with dysfunction of different organs. nevertheless, predictability of neuromuscular recovery may be given. thus, this study evaluates parameters to predict individual neuromuscular recovery in patients with uncommon diseases.	1999	10548961
joining rna molecules with t4 dna ligase.		1999	10549511
gemcitabine and cisplatin versus mitomycin, ifosfamide, and cisplatin in advanced non-small-cell lung cancer: a randomized phase iii study of the italian lung cancer project.	to compare gemcitabine and cisplatin (gc) with mitomycin, ifosfamide, and cisplatin (mic) chemotherapy in patients with stage iiib (limited to t4 for pleural effusion and n3 for supraclavicular lymph nodes) or stage iv non-small-cell lung cancer (nsclc). the end points were the evaluation of quality of life (qol), response rates, survival, and toxicity.	1999	10550150
vibriophage kvp40 and coliphage t4 genomes share a homologous 7-kbp region immediately upstream of the gene encoding the major capsid protein.	vibriophage kvp40, a large tailed dna phage morphologically similar to t-even coliphages, has a major capsid protein (mcp) homologous to the equivalent protein, gp23(*), of coliphage t4. the sequence analysis was extended to a 7-kbp region immediately upstream of the mcp gene encoding the precursor of mcp. the region as a whole was fairly homologous to the corresponding region of the t4 genome and contained 8 orfs homologous to t4 genes 17, 18, 19, 20, 67, 68, 21, and 22 in the same order as in ...	1999	10550672
wakame seaweed suppresses the proliferation of 7,12-dimethylbenz(a)-anthracene-induced mammary tumors in rats.	we examined the anti-tumor proliferation effects of wakame seaweed on 7,12-dimethylbenz(a)-anthracene (dmba)-induced rat mammary tumor. dmba was administered to 8-week-old female sprague-dawley rats, and rats which developed mammary tumors were assigned randomly to three groups. commercial rat feed was used in a control group (group i-a), and two feed mixtures were prepared, which contained commercial rat feed blended with wakame at 1.0% (group i-b) and 5.0% (group i-c) by weight. the respective ...	1999	10551319
high thoracic epidural anesthesia does not inhibit sympathetic nerve activity in the lower extremities.	sympathetic nerve activity was recorded in the leg during high thoracic epidural anesthesia with a segmental sensory blockade of the upper thoracic dermatomes to test the hypothesis that the sympathetic blockade accompanying thoracic epidural anesthesia includes caudal parts of the sympathetic nervous system.	1999	10551580
the analgesic action of nitrous oxide is dependent on the release of norepinephrine in the dorsal horn of the spinal cord.	the authors and others have demonstrated that supraspinal opiate receptors and spinal alpha2 adrenoceptors are involved in the analgesic mechanism for nitrous oxide (n2o). the authors hypothesize that activation of opiate receptors in the periaqueductal gray results in the activation of a descending noradrenergic pathway that releases norepinephrine onto alpha2 adrenoceptors in the dorsal horn of the spinal cord.	1999	10551592
characterization of the thyroxine-binding site of thyroxine-binding globulin by site-directed mutagenesis.	the principal transport protein for t4 in human blood, thyroxine-binding globulin (tbg), binds t4 with an exceptionally high affinity (ka = 10(10) m(-1)). its homology to the superfamily of the serpins has recently been used in the design of chimeric proteins, providing experimental evidence that an eight-stranded beta-barrel domain encompasses the ligand-binding site. we have now characterized the t4 binding site by site-directed mutagenesis. sequence alignment of tbg from several species revea ...	1999	10551780
potato performance in response to different mulches. 1. nitrogen metabolism and yield.	this work evaluates the impact that the application of different mulches exerts on nitrogen (n) metabolism in roots and leaves and on yield of potato tubers (solanum tuberosum var. spunta). the parameters analyzed in this experiment were root temperature; nitrate reductase and nitrite reductase activities; concentrations of nitrates, ammonium, amino acids, proteins, and organic n; dry weight of roots and leaves; and tuber yield. use of white polyethylene (t2) and white-black plastic (t3) promote ...	1999	10552541
analysis of the hereditary pancreatitis-associated cationic trypsinogen gene mutations in exons 2 and 3 by enzymatic mutation detection from a single 2.2-kb polymerase chain reaction product.	hereditary pancreatitis is associated with at least 2 mutations in the cationic trypsinogen gene. the purpose of the present study is to test the utility of t4 endonuclease vii for the detection of cationic trypsinogen r117h mutations. in addition, the possibility of screening for r117h, n21i, and a8v mutations in a single 2.2-kb polymerase chain reaction (pcr) product using t4 endonuclease vii was investigated.	1999	10553021
serum fructosamine concentration in cats with overt hyperthyroidism.	to determine the effect of hyperthyroidism on serum fructosamine concentration in cats.	1999	10553441
the influence of different intravascular volume replacement regimens on renal function in the elderly.	elderly patients are at risk of developing renal dysfunction. synthetic colloids are often used perioperatively, but they may have detrimental effects on renal function. in a prospective, randomized study, we assessed the influence of different intravascular volume replacement regimens on renal function in elderly (>65 yr) and younger (< 65 yr) patients without preoperative renal dysfunction who were undergoing major abdominal surgery. either 6% low molecular weight hydroxyethyl starch (hes) sol ...	1999	10553822
tumor angiogenesis predicts recurrence with normal serum carcinoembryonic antigen in advanced rectal carcinoma patients.	many studies have established the usefulness of serum carcinoembryonic antigen (cea) oriented serial monitoring for predicting recurrence and prognosis; however, few studies have so far investigated serum cea-negative recurrence. the aim of this study was to elucidate the nature of cea-negative recurrence regarding tumor angiogenesis. fifty-seven patients with t3/t4 rectal cancer were divided into the two groups according to the serum cea status. angiogenesis was defined as the intratumoral vess ...	1999	10554319
combined resection of the thoracic esophagus and thoracic descending aorta.	we conducted combined resection of the thoracic esophagus and thoracic descending aorta in 2 patients, one with advanced esophageal cancer with aortic invasion and the other aortoesophageal fistula caused by a false aortic aneurysm. combined resection of esophageal tumor and adjacent involved organs was conducted in 14 patients with a3:t4 esophageal cancer but none survived 3 years and resecting tumor-invaded organs did not improve patient survival. one major problem of combined resection of the ...	1999	10554419
can we get aids from mosquito bites?	human immunodeficiency virus, or hiv, is a human retrovirus that infects lymphocytes and other cells bearing the cd4 surface marker. the virus is transmitted primarily by sexual and parental routes. there are two ways blood feeding arthropods can spread disease, mechanically, by simple transfer of virus between hosts by contaminated mouth parts, or, biologically, which would require virus replication in arthropod tissues (especially salivary glands). there are some important factors which have p ...	1999	10554479
[low t3 syndrome in multiple trauma patients--a phenomenon or important pathogenetic factor?].	many nonthyroidal illnesses, such as major trauma, severe burn injury, sepsis or immune deficiency are associated with a reduced t3 concentration without increased serum tsh secretion. the pathopysiologic meaning of this phenomenon was controversely discussed since its investigation 20 years ago. the identification of the type i 5-iodthyronine-deiodinase as a selenoenzyme brought many new aspects into this discussion.	1999	10554534
force generation upon hydrostatic pressure release in tetanized intact frog muscle fibres.	single intact muscle fibres isolated from the tibialis anterior muscle of the frog were exposed to hydrostatic pressures of 1-10 mpa, at 2-4 degrees c and sarcomere length of 2.1-2.2 microm. the pressure was rapidly released (ca. 1 ms) to atmospheric level (0.1 mpa) during the plateau of a tetanic contraction (po) and the resultant tension (= force) transient examined. the pressure release induced tension transient consisted of an initial tension drop coincident with pressure release (ca. 4% po ...	1999	10555066
high prevalence of hypothyroidism in patients with primary pulmonary hypertension.	to characterize the prevalence of hypothyroidism in a population with primary pulmonary hypertension (pph).	1999	10555089
a new classification for malignant tumors involving the anterior skull base.	to propose our clinical classification of malignant ethmoid tumors and to compare it with the last american joint committee on cancer (ajcc)-union internationale contre le cancer (uicc) classification, published in 1997.	1999	10555698
prognostic factors in follicular carcinoma of the thyroid--a multivariate survival analysis.	multivariate studies of the diagnosis, treatment and prognosis of patients with follicular carcinoma of the thyroid gland are relatively scarce. the aim of our multivariate analysis was to study the factors associated with survival of patients with follicular carcinoma in slovenia, in an iodine-deficient region.	1999	10556007
substitution with selenomethionine can enhance the stability of methionine-rich proteins.	the availability of a series of phage t4 lysozymes with up to 14 methionine residues incorporated within the protein has made it possible to systematically compare the effect on protein stability of selenomethionine relative to methionine. wild-type lysozyme contains two fully buried methionine residues plus three more on the surface. the substitution of these methionine residues with selenomethionine slightly stabilizes the protein. as more and more methionine residues are substituted into the ...	1999	10556025
biochemical markers of bone turnover in patients with thyroid dysfunctions and in euthyroid controls: a cross-sectional study.	hyperthyroidism is associated with reduced bone mineral density. conflicting data exist regarding the effects of thyroxine therapy on bone metabolism. the aim of the present study was to assess changes in markers of bone turnover in thyroid dysfunction. a total of 28 patients with overt hyperthyroidism, eight patients with suppressed tsh levels (thyroid hormones within the euthyroid range, no t4 therapy), 25 euthyroid and four hypothyroid patients were included in the present study. hyperthyroid ...	1999	10556662
two new early bacteriophage t4 genes, repea and repeb, that are important for dna replication initiated from origin e.	two new, small, early bacteriophage t4 genes, repea and repeb, located within the origin e (orie) region of t4 dna replication, affect functioning of this origin. an important and unusual property of the orie region is that it is transcribed at early and late periods after infection, but in opposite directions (from complementary dna strands). the early transcripts are mrnas for repea and repeb proteins, and they can serve as primers for leading-strand dna synthesis. the late transcripts, which ...	1999	10559179
lymphocyte subsets and sister-chromatid exchanges in the students exposed to formaldehyde vapor.	the present report evaluates the effects of formaldehyde (fa) exposure on peripheral lymphocytes by using both genetic and immunological parameters. twenty-three non-smoking students in the study had inhalation exposure to 0.508 +/- 0.299 mg/m3 of fa for a period of 8 weeks (3h x 3 times each week) during anatomy classes. as for composition of lymphocyte subsets after fa exposure, significant increase was found in the percentage of cd19 (b cells), while significant decrease was observed in cd3 ( ...	1999	10560533
immunoglobulins of untreated graves' patients with or without thyrotropin receptor antibody (determined by porcine thyrocytes) universally elicit potent thyroid hormone-releasing activity in cultured human thyroid follicles.	thyrotropin receptor antibody (trab), comprising thyrotropin binding inhibitor immunoglobulin (tbii) and thyroid-stimulating antibody (tsab), both of which are conventionally determined using porcine thyrocytes in japan, is not always positive in patients with untreated graves' disease. to elucidate whether immunoglobulin g (igg) obtained from tbii/tsab-positive (+) or negative (-) graves' disease patients are responsible for hyperthyroidism, we investigated the thyroid hormone-releasing activit ...	1999	10560951
anaplastic thyroid cancer with transient thyrotoxicosis: case report and literature review.	a 55-year-old woman with anaplastic thyroid carcinoma presented with hyperthyroidism and neck swelling, hoarseness, and cervical lymphadenopathy. on physical examination, she was found to be clinically hyperthyroid with an enlarged, nontender multinodular goitre. her serum thyroid hormone levels confirmed hyperthyroidism and technetium-99m pertechnetate scan failed to visualize the thyroid gland. open biopsy showed an invasion of the thyroid gland by anaplastic thyroid carcinoma. the thyrotoxic ...	1999	10560959
impact of preoperative bimodality induction including twice-daily radiation on tumor regression and survival in stage iii non-small-cell lung cancer.	the objective of this prospective study was to assess the feasibility, toxicity, and efficacy of an intensive trimodality approach in stage iii non-small-cell lung cancer (nsclc).	1999	10561177
definitive chemoradiotherapy for t4 and/or m1 lymph node squamous cell carcinoma of the esophagus.	to investigate the efficacy and feasibility of concurrent chemoradiotherapy for locally advanced carcinoma of the esophagus.	1999	10561371
thyroid development in relation to the development of endothermy in the red-winged blackbird (agelaius phoeniceus).	we investigated the development of thyroid function during the transition to endothermy in red-winged blackbirds (agelaius phoeniceus). thermoregulatory capabilities of blackbirds improve markedly over their relatively short nestling period (10-12 days), with the most striking improvements occurring between days 6 and 8. we hypothesized that the development of endothermy in these birds is dependent in part on the development of thyroid function. we assessed thyroid development by measuring chang ...	1999	10562450
end results of a prospective trial on elective lateral neck dissection vs type iii modified radical neck dissection in the management of supraglottic and transglottic carcinomas. brazilian head and neck cancer study group.	either modified type iii radical neck dissection (mrnd) or lateral neck dissections (lnds) are considered valid treatments for patients with laryngeal carcinoma with clinically negative neck findings (n0). the object of this prospective study was to compare complications, neck recurrences, and survival results of elective mrnd and lnd on the management of laryngeal cancer patients. patients and methods this prospective randomized study began in 1990, and patient accrual was closed on december 19 ...	1999	10562681
cancer of the base of the tongue: past and future.	squamous cell carcinomas of the base of the tongue often are diagnosed at advanced stages, in a context of undernutrition with a history of smoking and alcoholism. the local treatment of these tumours is based on external irradiation, either alone or combined with brachytherapy, followed by salvage surgery in the case of failure. surgery was rarely performed as first-line treatment in our institution until 1992.	1999	10562689
a randomized trial comparing regimens of four reverse transcriptase inhibitors given together or cyclically in hiv-1 infection--the quattro trial. quattro steering committee.	to assess if cyclical therapy with four reverse transcriptase inhibitors is less toxic and as active, based on virological and immunological markers, as the same four drugs given together over a 64-week period.	1999	10563706
a chronic model for intravital microscopic study of microcirculatory disorders and leukocyte/endothelial cell interaction during normotensive endotoxemia.	sepsis-induced microvascular leukocyte/endothelial cell interaction may result in a deterioration of capillary perfusion that finally leads to septic organ dysfunction. the aim of the present study was to characterize a novel, sublethal, two-hit model of chronic systemic sepsis that allows the repeated analysis of microcirculation by intravital microscopy. in syrian golden hamsters the effect of a single i.v. endotoxin (lps, 2 mg/kg, e. coli) injection (sh-lps group, n = 5 animals) vs. a double ...	1999	10565610
effect of methimazole pretreatment on serum thyroid hormone levels after radioactive treatment in graves' hyperthyroidism.	radioiodine (131i) is the preferred definitive treatment for graves' hyperthyroidism. pretreatment with antithyroid drugs is often used to avoid thyroid hormone discharge after 131i ablation. however, this may represent an unnecessary increase in risk and costs. fifty-one patients with graves' disease were randomly assigned to receive 131i alone (28 patients) or 131i plus pretreatment with methimazole (30 mg/day; 23 patients). methimazole was interrupted 4 days before 131i therapy. serum t4, fre ...	1999	10566642
quantitative reverse transcription-polymerase chain reaction of circulating thyroglobulin messenger ribonucleic acid for monitoring patients with thyroid carcinoma.	patients with thyroid cancer are monitored for disease recurrence by measurement of serum thyroglobulin (tg) and iodine-131 (131i) scanning. to enhance sensitivity and to circumvent antibodies that interfere with tg immunoassays, we have developed rt-pcr assays that detect circulating thyroid messenger rna (mrna) transcripts. we now report results using a sensitive quantitative tg mrna assay (taqman; abi, foster city, ca) in comparison with immunoassay in patients previously treated for thyroid ...	1999	10566646
regulation of uncoupling protein-2 and -3 by growth hormone in skeletal muscle and adipose tissue in growth hormone-deficient adults.	the newly described uncoupling proteins, ucp2 and ucp3, may play a role in regulating energy expenditure (ee) in humans. gh deficiency (ghd) is associated with decreased lean body mass, increased adiposity, and reduced ee, which are reversed by gh treatment. in the present study we investigated whether gh treatment for 4 months influenced the expression of ucps in skeletal muscle and adipose tissue in 22 ghd patients who were investigated before and after gh (n = 11) or placebo (n = 11) treatmen ...	1999	10566652
streptococcus pyogenes isolated in portugal: macrolide resistance phenotypes and correlation with t types. portuguese surveillance group for the study of respiratory pathogens.	from january 1998 to june 1999, 302 clinical isolates of streptococcus pyogenes were collected from 10 microbiology laboratories in portugal. all strains were highly sensitive to penicillin (mic90 = 0.012 mg/liter). the prevalence of erythromycin resistance was 35.8% and of tetracycline resistance 41.4%. the majority (79.6 %) of erythromycin-resistant strains were of the mlsb constitutive resistance (cr) phenotype with high-level resistance to erythromycin (mic90 >256 mg/liter) and to clindamyci ...	1999	10566873
[spontaneous spinal cord herniation: a little-known cause of brown-sequard syndrome. report of two cases and review of the literature].	we report two cases of spinal cord herniation. the first one concerns a 28 year-old female who presented with an eighteen-month past history of spasticity of the right lower limb associated with somesthetic sensorial disturbances of the left side with a t4 level, related to a t3-t4 spinal cord herniation. the second case concerned a 58 year-old female with a past history of a left lower limb pyramidal syndrome associated with somesthetic and proprioceptive sensibilities disturbances of the right ...	1999	10567963
response of the pituitary and thyroid to tropic hormones in sprague-dawley versus fischer 344 male rats.	modulation of endocrine function is frequently a confounding factor in the interpretation of chronic rodent toxicology studies. of particular interest are agents that cause deviation of thyroid hormone homeostasis and result in thyroid cancer for rodents. an endocrine challenge test (ect), commonly used to study endocrine organ health in human and veterinary medicine, quantifies the response of the thyroid to tropic hormones. this study compared the response of fischer (f344) and sprague-dawley ...	1999	10568704
lipids in rat liver submitted to acute and chronic hyperthyroidism.	the effect of acute and chronic hyperthyroidism was studied in serum and liver lipids in rats. wistar adult female rats were separated into three groups. the first group, injected with saline solution was used as control (co), while the second and third were injected daily with tetraiodothyronine (t4) 10 microg/100 g body weight; the second group (ht-i) for one week and the third group (ht-ii) for five weeks. in ht-i, serum t4 level was higher than in ht-ii. triiodothyronine (t3) concentration i ...	1999	10569253
intra-strand biases in bacteriophage t4 genome.	in bacteriophage t4, a major portion of dna replication is initiated at random along the map, although several proven and putative origins have been described for early replication. in order to analyze the contribution of transcription and translation as well as dna replication to intra-strand bias from a = t and g = c, we examined the pattern of the intra-strand biases in the first, second, and third codon positions of the coding regions as well as the intergenic regions of the t4 genome. we fo ...	1999	10570984
evaluation of thyroid function in lithium-naive bipolar patients.	a high prevalence of thyroid hypofunction has been found in bipolar patients. however, the samples used in previous studies included a high percentage of patients in treatment with lithium and carbamazepine. since the use of these drugs may explain the high prevalence of thyroid disturbances found in bipolar patients, we designed the present study to assess thyroid function in a sample of bipolar patients who had not been treated previously with lithium or carbamazepine. patients included in the ...	1999	10572366
limited t4 exonuclease activity and partial fill-in expand insertion site options for pcr subcloning.		1999	10572636
association of prevalent vertebral fractures, bone density, and alendronate treatment with incident vertebral fractures: effect of number and spinal location of fractures. the fracture intervention trial research group.	vertebral fractures are the most common osteoporotic fracture and are associated with significant pain and disability. prior vertebral fracture and low bone mineral density (bmd) are strong predictors of new vertebral fracture. using data from 6082 women, ages 55-80 years, in the fracture intervention trial (a randomized, placebo-controlled trial of the antiresorptive agent, alendronate), we explored the association of the number of prior vertebral fractures with the risk of new fractures and wh ...	1999	10574584
glycyl radical enzymes: a conservative structural basis for radicals.	the first structures of glycyl radical enzymes, the anaerobic ribonucleotide reductase from bacteriophage t4 and pyruvate formate lyase from escherichia coli, have been recently determined. this work provides new insights into the structure and chemistry of glycyl radical sites.	1999	10574800
plasma insulin-like growth factor-i and serum igf-binding protein 3 can be associated with the progression of breast cancer, and predict the risk of recurrence and the probability of survival in african-american and hispanic women.	in vitro studies have shown that insulin-like growth factor (igf) is a mitogen for breast cancer cells. however, the associations of plasma igf-i with tumor histopathology in high-risk groups need further investigation. we hypothesize that plasma igf-i and serum igfbp3 concentrations in breast cancer patients may provide useful information on the progression of their disease, and determine the probability of recurrence and survival. we have carried out a retrospective study on 130 minority breas ...	1999	10575321
positive impact on surgical treatment for asymptomatic patients with esophageal carcinoma.	the prognosis of patients with esophageal carcinoma remains unsatisfactory. the purpose of this study was to clarify the clinicopathologic characteristics of asymptomatic patients.	1999	10576360
predictive value of decreased p27kip1 protein expression for the recurrence-free and long-term survival of prostate cancer patients.	the p27kip1 gene has been identified as inductor of cell cycle arrest at the g1 checkpoint to prevent entry of somatic cells into the s phase of the cell cycle when substantial dna damage has occurred. it has been suggested that decreased expression of the p27kip1 protein may contribute to the development of human malignancies due to loss of critical antiproliferative mechanisms. in the present study, 95 specimens (t1-t4) from 95 randomly selected patients undergoing radical prostatectomy at the ...	1999	10576664
radiotherapy in bladder cancer.	in the present review, we have evaluated the outcome of radiotherapy in patients with bladder cancer. the exact value of radical radiotherapy is difficult to establish because changes in treatment techniques and selection of patients have biased the results. the 5-year survival rates are reported to be 35-71% in t1 tumors, 27-59% in t2 tumors, 10-38% in t3 tumors and 0-16% in t4 tumors. several other factors, like performance status and hemoglobin level, are important for the outcome. morbidity ...	1999	10577680
nutritional assessment in head injured patients through the study of rapid turnover visceral proteins.	nutritional monitoring of rapid turnover visceral protein is important in the recognition of malnutrition in patients admitted to the intensive care unit (icu). we studied prealbumin and retinol-binding protein in patients who received three different kinds of artificial nutrition in order to evaluate the appropriateness of artificial nutrition.	1999	10578025
complete cervical or thoracic spinal cord transections delay gastric emptying and gastrointestinal transit of liquid in awake rats.	to determine the changes on gastric emptying and gastrointestinal transit of liquid throughout the first week after spinal cord transection (sct) in rats.	1999	10578251
hormonal profiles correlated with season, cold, and starvation in rana catesbeiana (bullfrog) tadpoles.	bullfrog (rana catesbeiana) tadpoles are of value to amphibian researchers because of their large size, and year-round availability due to overwintering in many latitudes. concern over a possible difference in hormonal parameters in tadpoles obtained at different times of the year prompted us to investigate thyroid gland secretion in vitro, plasma and ocular melatonin, and plasma corticosteroids in late pre- to early prometamorphic larvae on 12l:12d. winter tadpoles exposed to 22 degrees c for 3 ...	1999	10579655
human immunodeficiency virus infection in vitro activates naturally integrated human papillomavirus type 18 and induces synthesis of the l1 capsid protein.	human papillomavirus (hpv) infections are prevalent in human immunodeficiency virus (hiv)-positive individuals. to highlight the effect of hiv on hpv expression, hpv-18-positive hiv-permissive hela-t4 cells were either infected with hiv-1 or treated with tat or with the cytokines il-1alpha, il-1beta, il-6 and tnf-alpha. the presence of hpv-18 e1 (early) and l1 (late) transcripts was then determined by dot-blot or northern blot hybridization with e1 and l1 or with genomic hpv-18 dna probes, respe ...	1999	10580055
effect of streptozotocin-induced diabetes mellitus on type 1 deiodinase (d1) in inherited d1-deficient mice.	we investigated the effects of streptozotocin (stz)-induced diabetes on thyroid hormone levels, type 1 deiodinase (d1) activity and messenger rna (mrna) levels in inherited d1 deficient c3h mice in a comparative manner with control c57 mice. the apparent maximum velocity (vmax) d1 values in c3h mice were 3% (liver) and 26% (kidney) of those in c57 mice. in c3h mice, similar serum t3, slightly higher t4, and 2.6-fold higher rt3 levels were observed compared with c57 mice. in stz-induced diabetes, ...	1999	10580741
levothyroxine-induced liver dysfunction in a primary hypothyroid patient.	here we report a case of levothyroxine-induced liver dysfunction. t4 (levothyroxine) has been more commonly used for the treatment of hypothyroidism than t3 active hormone (triiodothyronine), because with the former drug a stabler plasma concentration is obtained after oral administration. although there are few reports on levothyroxine-induced liver dysfunction, we treated a primary hypothyroid patient with high serum aminotransferase after administration of levothyroxine. liver dysfunction was ...	1999	10580751
gene amplification as a common cause of inherited thyroxine-binding globulin excess: analysis of one familial and two sporadic cases.	t4-binding globulin (tbg) is the major thyroid hormone transport protein in humans. inherited abnormalities in the level of serum tbg have been classified as partial deficiency, complete deficiency and excess. a single nucleotide deletion or substitution in the tbg gene, located on xq22, has been detected in partial and complete deficiencies. as for inherited tbg excess, the gene amplification has been recognized in two japanese families recently. in this study, an additional three japanese fami ...	1999	10580756
primary hypothyroidism associated with leishmaniasis in a dog.	a case of primary hypothyroidism associated with leishmaniasis is described in a four-year-old, male yorkshire terrier. clinical diagnosis of hypothyroidism was confirmed by a low baseline serum tetraiodothyronine (t4), a reduced response to thyroid-stimulating hormone (tsh) stimulation, an increased serum tsh concentration, and scintigraphic thyroid gland examination. examination of a thyroid biopsy showed many leishmania amastigotes, both inside and outside of macrophages, together with signs ...	1999	10580908
role of exonucleolytic degradation in group i intron homing in phage t4.	homing of the phage t4 td intron is initiated by the intron-encoded endonuclease i-tevi, which cleaves the intronless allele 23 and 25 nucleotides upstream of the intron insertion site (is). the distance between the i-tevi cleavage site (cs) and is implicates endo- and/or exonuclease activities to resect the dna segment between the is and cs. furthermore, 3' tails must presumably be generated for strand invasion by 5'-3' exonuclease activity. three experimental approaches were used to probe for ...	1999	10581261
intron homing with limited exon homology. illegitimate double-strand-break repair in intron acquisition by phage t4.	the td intron of bacteriophage t4 encodes a dna endonuclease that initiates intron homing to cognate intronless alleles by a double-strand-break (dsb) repair process. a genetic assay was developed to analyze the relationship between exon homology and homing efficiency. because models predict exonucleolytic processing of the cleaved recipient leading to homologous strand invasion of the donor allele, the assay was performed in wild-type and exonuclease-deficient (rnh or dexa) phage. efficient hom ...	1999	10581262
giant cervico-thoracic schwannoma with long clinical history. case report.	an unusual case of a giant intraspinal schwannoma in a 45-year-old woman with 14-year history of preoperative symptoms was presented. mri of the spine revealed an intradural, extramedullary tumor extending from the intervertebral space c4/c5 to t4 vertebral body level (2 x 1.2 x 12 cm) and filling almost the entire spinal canal. microscopical examination showed a typical neurinoma pattern with two distinct zones of antoni a and antoni b tissue. some areas exhibited nuclear atypia and hyperchroma ...	1999	10581856
[vascular pedicle flap of the thyroid or submandibular gland in the reconstruction following partial laryngectomy].	the paper presents the merits and drawbacks of vascular pedicled flaps of thyroid and submandibular glands in the reconstructive surgery following partial laryngectomy (combined with the resection of tongue base if necessary), as well as the results of clinical, radiological, radionucleoloid++ and histological investigation carried out in 81 cases with various extend of neoplasm (t1-t4). the average observation period was 8.2 (min. 1, max. 14) years. the discussed flaps were satisfactorily elast ...	1999	10581945
[the level of diagnosis and treatment results in nasopharyngeal cancer patients].	a group of twenty patients with nasopharyngeal cancer treated in lower silesian oncological centre from year 1991 to 1995 have been presented. in seven of them the extent of the primary tumor was small (t1 or t2), but the rest of them (thirteen) had large extent of it (t3 or t4). in seven patients metastases to the cervical lymphonodes were not observed. the histopathology exam showed in eight cases well differentiated carcinoma, in another eight--lymphoepitelioma, in three--undifferentiated car ...	1999	10581947
docetaxel (taxotere) in the neo-adjuvant setting in non-small-cell lung cancer.	there is a potential for neo-adjuvant chemotherapy to provide significant benefit in outcome for patients with locally advanced non-small-cell lung cancer (nsclc). patients with n2/t3 nsclc have a poor prognosis when treated by surgery alone since micrometastases result in relapse in the majority of cases. the same is true of patients with n3/t4 disease treated only with radiotherapy. systemic therapy is therefore required, and cisplatin-based induction chemotherapy prior to surgery or radiother ...	1999	10582143
[amiodarone and thyroid disorders--prospective study and review of the literature].	a well-known adverse effect of amiodarone is the induction of thyroid dysfunction. the numerous studies evaluating the incidence of amiodarone-induced thyroid dysfunction have given very dissimilar results, because of the variability in the criteria of hyper- and hypothyroidism, on the one hand, and in the iodine intake of the studied population on the other hand. the aim of our study was to evaluate the incidence of amiodarone-induced thyroid dysfunction. one hundred and twelve patients without ...	1999	10582476
changes in serum apolipoprotein concentrations after l-thyroxine therapy in infants with congenital hypothyroidism.	to further characterize the impact of thyroid hormones on the serum lipid profile, we studied serum apolipoproteins in infants with congenital hypothyroidism before and after l-thyroxine (l-t4) replacement therapy. serum high-density lipoprotein cholesterol (hdlc) decreased after l-t4 therapy. total cholesterol (tc) and low-density lipoprotein cholesterol (ldlc) levels did not change significantly after therapy. two months after l-t4 replacement therapy, serum apolipoprotein a-i (apo a-i), c-iii ...	1999	10582538
postpartum thyroid dysfunction: clinical assessment and relationship to psychiatric affective morbidity.	postpartum thyroid dysfunction (pptd), diagnosed using biochemical criteria, is usually transient with a wide range of reported prevalence rates. the specific clinical and psychiatric morbidity associated with pptd is still uncertain. the aims of the study were to determine the point prevalence of pptd in australian women at 6 months postpartum and to assess the specific clinical and psychiatric morbidity in these women.	1999	10583309
abnormal circadian rhythm and increased non-pulsatile secretion of thyrotrophin in sheehan's syndrome.	it has previously been shown that patients with postpartum pituitary necrosis (sheehan's syndrome, ss) have paradoxically increased tsh levels and loss of the nocturnal tsh surge. this study sought to determine the circadian and pulsatile characteristics of tsh secretion underlying those abnormalities.	1999	10583310
iopanoic acid-induced decrease of circulating t3 causes a significant increase in gh responsiveness to gh releasing hormone in thyrotoxic patients.	thyroid hormones participate in gh synthesis and secretion, and an impaired gh response to many pharmacological stimuli, including gh releasing hormone (ghrh), has been found in thyrotoxicosis. although the mechanisms involved in this process have not been fully elucidated, there is evidence that thyroid hormones could act at both hypothalamic and pituitary levels. there are no data in the literature about the effect of an acute reduction of circulating t3 levels on gh secretion in hyperthyroidi ...	1999	10583313
[thyrotoxic periodic paralysis. a report of 2 cases].	thyrotoxic hypokalemic periodic paralysis is characterized by recurrent episodes of motor weakness of variable intensity associated with thyroid overactivity. it is usually associated with low plasma potassium levels and is often precipitated by physical activity or ingestion of carbohydrates.	1999	10584263
efflux of t4 from the in situ perfused liver of rainbow trout: effect of t4, dithiothreitol and cysteine in the perfusate.	a cortland saline-perfused rainbow trout (oncorhynchus mykiss) liver model was used to study aspects of t4 efflux from the intact organ system. there was a consistent efflux of t4 in the absence of t4 in the perfusate, and the t4 efflux was increased in the presence of t4 in the perfusate, but the efflux was not t4-dose dependent. the addition of the thiol-containing compound dithiothreitol (dtt, 2 mm) to the perfusate had no significant effect on the flux of t4 from the liver, whereas the addit ...	1999	10584300
recurrent fetal thyrotoxicosis in a woman with graves' disease: case report.	the thyroid stimulating immunoglobulins are generally believed to be the cause of hyperthyroidism in graves' disease. placental transfer of these antibodies from a mother with autoimmune thyroid disease can result in fetal thyroid disorders. we report the case of a 31-year-old woman who had a history of graves' disease. she received thyroxine therapy for post thyroidectomy hypothyroidism. two years after the thyroidectomy, she became pregnant. unfortunately, intrauterine fetal death occurred in ...	1999	10584424
a multidisciplinary surgical approach to superior sulcus tumors with vertebral invasion.	vertebral body invasion by superior sulcus tumor has traditionally been considered a contraindication to surgical resection. attempts at definitive radiation or chemoradiation have not been successful. recent advances in spinal instrumentation have allowed more complete resection of vertebral body tumors. we, therefore, reviewed our recent experience with vertebral resection of superior sulcus tumors.	1999	10585058
in vitro reconstitution of the bacteriophage t4 clamp loader complex (gp44/62).	the clamp loader complex (clc) of bacteriophage t4 is essential for viability and has analogs in both prokaryotes and eukaryotes. the gp44 and gp62 subunits of the t4 clc, in a 4:1 ratio, tightly associate such that the two proteins co-purify. using transformed escherichia coli, we were able to demonstrate for the first time purification of the unique protein gp62 in the absence of gp44. we experimentally determined the isoelectric point for the individual subunits. an in vitro physical interact ...	1999	10585481
cyclin d1, glutathione s-transferase, and cytochrome p450 genotypes and outcome in patients with upper aerodigestive tract cancers: assessment of the importance of individual genes using multivariate analysis.	gst, cyp, and ccnd1 genotypes have been associated with outcome in several cancers. accordingly, we have examined, in patients with one squamous cell carcinoma (scc) of the head and neck, associations between gstm1, gstt1, gstm3, gstp1, cyp2d6, cyp1a1, cyp2e1, and ccnd1 genotypes and the outcome parameters, tumor extension, histological grade, and presence of nodes. we used logistic regression to study, first, each gene individually and, second, in a step-wise model that included all of the gene ...	1999	10498401
prognostic value of le(y) and h antigens in oral tongue carcinomas.	to investigate the prognostic significance of the carbohydrate epitopes h and le(y) and their relationship with proliferation and apoptosis.	1999	10499058
bis[platinum(ii)] and bis[platinum(iv)] complexes with optically active bis(vicinal-1,2-diamines) and their interaction with dna.	bis[platinum(ii)] [cl2pt(ll)ptcl2] complexes 2,5 and 8 with chiral non-racemic ligands: 1a-c (ll = (r,r), (s,s) and (r,s) n,n'-bis(3,4-diaminobutyl)hexanediamide); 4a,b (ll = (r,r) and (s,s) n,n'-bis[3,4-bis(diaminobutyl)] urea); 7a-d (ll' = (r,r), (s,s), (r,s) and (s,r) 4,5-diamino-n-(3,4-diaminobutyl) pentanamide) and bis[platinum(iv)] complex 10-13 with ligands 1a,b and 4a,b have been prepared and characterized by ir, 1h, 13c and 195pt nmr spectra. the interactions of 2a-c, 5a, 5b, 8a-d and 1 ...	1999	10499290
iodine speciation in biological samples by capillary electrophoresis-inductively coupled plasma mass spectrometry.	a hyphenation of capillary electrophoresis (ce) to inductively coupled plasma mass spectrometry (icp-ms) was employed for the speciation of iodine. the separation method used a buffer sandwich of phosphate (ph 2.3), naoh, sodium dodecyl sulfate (sds) and borate buffer (ph 8.3) for stacking, aiming at sufficient separation of iodide, iodate, thyroxine (t4) and triiodothyronine (t3). these four iodine species were separated within 15 min and subsequently detected during a pressure-driven detection ...	1999	10499349
prognostic significance of fas and fas ligand expressions in human esophageal cancer.	esophageal carcinomas have recently been shown to express fas ligand (fasl) and down-regulate fas to escape from host immune surveillance. however, the prognostic importance of fas/fasl and their correlation with clinicopathological characteristics are yet to be delineated in this highly malignant carcinoma. specimens from 106 esophageal squamous cell carcinoma patients were used for immunohistochemical evaluation of fas, fasl, and cd8 expressions. fifty-two (49%) and 34 (32%) patients were posi ...	1999	10499620
predicting tumor failure in prostate carcinoma after definitive radiation therapy: limitations of models based on prostate-specific antigen, clinical stage, and gleason score.	in this report, we use new patient data to test three popular models developed to predict the outcome of definitive radiation therapy. the data come from 240 men with localized prostate cancer and who were treated with definitive radiation therapy at a community hospital. all three models tested were based on the three commonly available variables of pretreatment prostate-specific antigen (psa), gleason score, and tumor stage, and we used the cox proportional hazards model and the logistic regre ...	1999	10499622
efficacy of simulated intravenous test dose in the elderly during general anesthesia.	reliability of detecting unintentional intravascular injection of the epinephrine-containing test dose is improved by decreasing the heart rate (hr) threshold to 10 beats/min during combined epidural and general anesthesia. we have tested whether this modified hr criterion is still applicable in the anesthetized elderly patients.	1999	10499748
hiv-1 long terminal repeat modulation by glucocorticoids in monocytic and lymphocytic cell lines.	glucocorticoid gene regulation can be carried out through direct binding of glucocorticoid receptor to glucocorticoid responsive elements (gre), regulating directly gene transcription and modulating some signaling pathways. the human immunodeficiency virus type 1 (hiv-1) expression can be activated by different immunomodulators through binding of particular nuclear factors to its long terminal repeat (ltr). in order to investigate the effect of glucocorticoids in pathways that activate hiv-1 exp ...	1999	10500286
splanchnic perfusion during controlled hypotension with haemodilution under isoflurane anaesthesia in elderly patients.	we investigated the effects of controlled hypotension with haemodilution under isoflurane anaesthesia on splanchnic perfusion in elderly patients. we determined the intramucosal ph using gastric tonometry in 28 patients scheduled for hip surgery. patients without cardiac disease were assigned to two groups according to age. group a (adult patients, n = 14) included patients aged less than 60 years (range 29-58 years, 47 +/- 11 years, mean +/- sd) and group b (elderly patients, n = 14) more than ...	1999	10500940
measurement of vertebral body heights: ex vivo comparisons between morphometric x-ray absorptiometry, morphometric radiography and direct measurements.	morphometric x-ray absorptiometry (mxa) offers some potential advantages over spinal radiography for the quantitative evaluation of vertebral fractures in individuals with spinal osteoporosis. this ex vivo study examined the accuracy of mxa and quantitative morphometry (qm) for the evaluation of vertebral height, in relation to direct measurements from cadaveric vertebral columns. spinal radiographs and mxa scans were obtained from nine cadaveric vertebral columns (mean age at death 64 years). a ...	1999	10501773
identification of vertebral deformities in men: comparison of morphometric radiography and morphometric x-ray absorptiometry.	osteoporotic vertebral deformities may be detected by morphometric radiography (mr) using spinal radiographs, and by morphometric x-ray absorptiometry (mxa) using dual-energy x-ray absorptiometry. reference values for mr may not be appropriate for mxa, and reference values may be affected by gender and age. the aims of this study were to (1) compare mean deformity of vertebral height ratios for mr and mxa in men, (2) compare mean deformity for mxa in men and women, (3) compare mean wedge angle m ...	1999	10501798
serum fructosamine concentrations in hyperthyroid cats.	serum fructosamine concentrations were measured in 35 healthy cats and in 30 hyperthyroid cats before and 30 days after curative radioiodine ((131)i) treatment. hyperthyroid cats were divided into those with 30 day post-treatment total thyroxine (t4) concentrations within (eut4) or below (hypot4) the reference range. the median (semi-interquartile range, sir) fructosamine concentration was significantly lower in hyperthyroid compared with healthy cats (295. 0 (18.5) micromol l(-1)) both before ( ...	1999	10502488
[the laboratory diagnosis of autoimmune thyroid diseases in those who worked in the cleanup of the aftermath of the accident at the chernobyl atomic electric power station].	serum antibodies to thyroid tissue antigens were tested by enzyme immunoassay and the thyroid hormonal status was analyzed in 80 liquidators of the chernobyl accident consequences. antibodies to thyroglobulin in concentration of 160.7 +/- 10.5 iu/ml were detected in 11 (13.7%) subjects 11-12 years after the accident. antibodies to thyroperoxidase were detected in this cohort: the reaction was positive in 2.5% cases and weakly positive in 5%. groups of liquidators with changed thyroid functional ...	1999	10502927
gugulu (commiphora mukul) induces triiodothyronine production: possible involvement of lipid peroxidation.	an investigation was made to find out the importance of gugulu (commiphora mukul) in thyroid function of mice and to reveal the possible involvement of lipid peroxidation (lpo), if any. while no marked change in the concentrations of serum thyroxine (t4) was observed, triiodoth yronine (t3) concentration and t3/t4 ratio were enhanced following the administration of gugulu extract (0.2 g/kg b. wt./d for 15 days). a concomitant decrease in lpo was also noticed in liver, the principal site of t3 ge ...	1999	10503949
clinical characteristics of amiodarone-induced thyrotoxicosis and hypothyroidism in japan.	since amiodarone was introduced in japan in 1992, the incidence of the drug-induced thyroid dysfunction has been increasing. we studied the thyroid function of 13 patients with amiodarone-induced thyrotoxicosis (ait) and 11 patients with amiodarone-associated hypothyroidism (aah) who had been referred to our institute in the last 6 years. ait and aah developed after 39+/-21 and 20+/-16 months of amiodarone treatment, respectively. one patient developed aah followed by ait. the ait ranged from su ...	1999	10503998
productive, physiological and biochemical changes in imported and locally born friesian and holstein lactating cows under hot summer conditions of egypt.	twenty each of healthy lactating friesian and holstein cows were studied. they were of similar age and body weight, nonpregnant in their third lactation and 80-100 days post partum. the investigation was carried out for 8 weeks on two farms at the same time during the hot summer season in egypt. the first farm was in demietta (north east of the nile delta, 31 degrees 40' n) on 20 friesian cows and the second was in fakous (east of the nile delta, 30 degrees 40' n) on 20 holstein cows. on each of ...	1999	10504103
folding of coliphage t4 short tail fiber in vitro. analysing the role of a bacteriophage-encoded chaperone.	the morphogenesis of the escherichia coli bacteriophage t4 depends on the presence of helper proteins which are not components of the mature virion. two bacteriophage-encoded proteins, p57 and p38, are required for the assembly of the bacteriophage t4 tail fibers. in the absence of p57, two polypeptides of the long fiber (p34 and p37) and that of the short tail fiber (p12) fail to trimerize. instead they form water-insoluble aggregates. co-expression of the genes 12 and 57 in vivo caused the for ...	1999	10504409
bacteriophage t4 resistance to lysis-inhibition collapse.	lysis inhibition is a mechanism of latent-period extension and burst-size increase that is induced by the t4 bacteriophage adsorption of t4-infected cells. mutants of t4 genes imm, sp and 5 (specifically the ts1 mutant of 5) display some lysis inhibition. however, these mutants experience lysis-inhibition collapse, the lysis of lysis-inhibited cells, earlier than wild-type-infected cells (i.e. their collapse occurs prematurely). lysis from without is a lysis induced by excessive t4 adsorption. g ...	1999	10505404
selective v2-receptor vasopressin antagonism decreases urinary aquaporin-2 excretion in patients with chronic heart failure.	aquaporin-2 (aqp-2), a water channel located on the apical membrane of collecting duct cells, regulates water reabsorption under the control of vasopressin (avp). using an antibody directed to human aqp-2, a quantitative western blot analysis was performed to determine the collecting duct responsiveness to an oral, nonpeptide, v2 receptor antagonist (vpa-985) in patients with chronic nyha ii and iii heart failure. standards were derived by conjugating the immunizing peptide to maleimide-activate ...	1999	10505693
[accuracy and dose dependency of the train-of-four count].	the estimation of a nondepolarizing neuromuscular block using the train-of-four (tof) count shows wide differences compared to the mechanomyographic measurement. the purpose of this study was to evaluate the clinical significance of these differences.	1999	10506315
[thoracoscopy-guided management of the "anterior column". methods and results].	anterior thoracoscopic interbody stabilization and fusion was performed in 163 patients. lesions treated were located between t4 and l3, most frequently occurring at t12/ l2. operative time decreased dramatically as experience was gained with the procedure. 2 patients early in the series successfully were converted to an open procedure. one positioning related pressure harm on the thoracodorsal nerve and one irritation of the l1 root at the entrance site were both transitory. postoperative contr ...	1999	10506375
pain and reduced mobility in complex regional pain syndrome i: outcome of a prospective randomised controlled clinical trial of adjuvant physical therapy versus occupational therapy.	there are no adequate comparative studies on physical therapy (pt) versus occupational therapy (ot) in patients with complex regional pain syndrome i (crps i). therefore, we conducted a prospective randomised clinical trial to assess their effectiveness. the outcomes regarding reducing pain and normalising active range of motion (arom) are discussed. included in the study were 135 patients who had been suffering from crps i of one upper extremity for less than one year. they were randomly assign ...	1999	10506674
frequency of noncontiguous lymph node involvement in patients with resectable nonsmall cell lung carcinoma.	this study was undertaken to investigate the patterns of lymph node spread and the frequency of involvement of noncontiguous lymph node stations in patients with nonsmall cell lung carcinoma who had complete surgical resection.	1999	10506699