Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| reciprocal regulation of resistance-nodulation-division efflux systems and the cpx two-component system in vibrio cholerae. | the cpx two-component regulatory system has been shown in escherichia coli to alleviate stress caused by misfolded cell envelope proteins. the vibrio cholerae cpx system was previously found to respond to cues distinct from those in the e. coli system, suggesting that this system fulfills a different physiological role in the cholera pathogen. here, we used microarrays to identify genes that were regulated by the v. cholerae cpx system. our observations suggest that the activation of the v. chol ... | 2014 | 24799626 |
| the transcription factor mlc promotes vibrio cholerae biofilm formation through repression of phosphotransferase system components. | the phosphoenol phosphotransferase system (pts) is a multicomponent signal transduction cascade that regulates diverse aspects of bacterial cellular physiology in response to the availability of high-energy sugars in the environment. many pts components are repressed at the transcriptional level when the substrates they transport are not available. in escherichia coli, the transcription factor mlc (for makes large colonies) represses transcription of the genes encoding enzyme i (ei), histidine p ... | 2014 | 24769694 |
| structure of bacterial cytoplasmic chemoreceptor arrays and implications for chemotactic signaling. | most motile bacteria sense and respond to their environment through a transmembrane chemoreceptor array whose structure and function have been well-studied, but many species also contain an additional cluster of chemoreceptors in their cytoplasm. although the cytoplasmic cluster is essential for normal chemotaxis in some organisms, its structure and function remain unknown. here we use electron cryotomography to image the cytoplasmic chemoreceptor cluster in rhodobacter sphaeroides and vibrio ch ... | 2014 | 24668172 |
| characterization of the rstb2 protein, the dna-binding protein of ctxϕ phage from vibrio cholerae. | the low abundant protein rstb2, encoded in the rs2 region of ctxϕ, is essential for prophage formation. however, the only biochemical activity so far described is the single/double-stranded dna-binding capacity of that protein. in this paper, a recombinant rstb2 (rrstb2) protein was overexpressed in e. coli with a yield of 58.4 mg l(-1) in shaken cultures, lb broth. the protein, purified to homogeneity, showed an identity with rrstb2 by peptide mass fingerprinting. the apparent molecular weight ... | 2014 | 24643345 |
| memories of a virulent past. | studies on bacterial virulence and host-pathogen interactions usually rely on type strains isolated from human or veterinary populations. for instance, the el tor strain n16961 has been extensively used to characterize vibrio cholerae virulence, while e2348/69 is a default choice for studies on enteropathogenic e. coli interactions with host cells. subsequent to isolation, such strains are passaged under laboratory conditions in rich medium, and often genetically manipulated to induce specific m ... | 2014 | 24637797 |
| differential requirement for pbp1a and pbp1b in in vivo and in vitro fitness of vibrio cholerae. | we investigated the roles of the vibrio cholerae high-molecular-weight bifunctional penicillin binding proteins, pbp1a and pbp1b, in the fitness of this enteric pathogen. using a screen for synthetic lethality, we found that the v. cholerae pbp1a and pbp1b proteins, like their escherichia coli homologues, are each essential in the absence of the other and in the absence of the other's putative activator, the outer membrane lipoproteins lpoa and lpob, respectively. comparative analyses of v. chol ... | 2014 | 24614657 |
| ultra-sensitive, high-throughput detection of infectious diarrheal diseases by portable chemiluminescence imaging. | this paper describes a rapid, ultra-sensitive, and high-throughput pathogenic dna identification strategy for infectious diarrheal diseases diagnosis. this strategy is based on specific dna hybridization and horseradish-peroxidase-catalyzed chemiluminescence (cl) detection. probe dna strands are covalently immobilized on the aldehyde-group-modified slide and hybridized with biotin-modified target dna strands. horseradish-peroxidase (hrp) is then combined with the target dna via a biotin-streptav ... | 2014 | 24534578 |
| high number of diarrhoeal co-infections in travellers to benin, west africa. | travellers' diarrhoea (td) is the most frequent health problem among travellers to the tropics. using routine techniques, the aetiology mostly remains unresolved, whereas modern molecular methods enable reducing the number of equivocal cases considerably. while many studies address the aetiology of td in asian, central american and north african tourist resorts, only few focus on western africa. | 2014 | 24521079 |
| evaluation in mice of a conjugate vaccine for cholera made from vibrio cholerae o1 (ogawa) o-specific polysaccharide. | protective immunity against cholera is serogroup specific. serogroup specificity in vibrio cholerae is determined by the o-specific polysaccharide (osp) of lipopolysaccharide (lps). generally, polysaccharides are poorly immunogenic, especially in young children. | 2014 | 24516685 |
| domain isolation, expression, purification and proteolytic activity of the metalloprotease prtv from vibrio cholerae. | the metalloprotease prtv from vibrio cholerae serves an important function for the bacteria's ability to invade the mammalian host cell. the protein belongs to the family of m6 proteases, with a characteristic zinc ion in the catalytic active site. prtv constitutes a 918 amino acids (102 kda) multidomain pre-pro-protein that so far has only been expressed in v. cholerae. structural studies require high amounts of soluble protein with high purity. previous attempts for recombinant expression have ... | 2014 | 24492010 |
| identification of a membrane-bound transcriptional regulator that links chitin and natural competence in vibrio cholerae. | vibrio cholerae is naturally competent when grown on chitin. it is known that expression of the major regulator of competence, tfox, is controlled by chitin; however, the molecular mechanisms underlying this requirement for chitin have remained unclear. in the present study, we identify and characterize a membrane-bound transcriptional regulator that positively regulates the small rna (srna) tfor, which posttranscriptionally enhances tfox translation. we show that this regulation of the tfor pro ... | 2014 | 24473132 |
| 193 nm ultraviolet photodissociation mass spectrometry for the structural elucidation of lipid a compounds in complex mixtures. | here we implement ultraviolet photodissociation (uvpd) in an online liquid chromatographic tandem mass spectrometry (ms/ms) strategy to support analysis of complex mixtures of lipid a combinatorially modified during development of vaccine adjuvants. uvpd mass spectrometry at 193 nm was utilized to characterize the structures and fragment ion types of lipid a from escherichia coli, vibrio cholerae, and pseudomonas aeruginosa using an orbitrap mass spectrometer. the fragment ions generated by uvpd ... | 2014 | 24446701 |
| importance of cholera and other etiologies of acute diarrhea in post-earthquake port-au-prince, haiti. | we estimated the proportion of diarrhea attributable to cholera and other pathogens during the rainy and dry seasons in patients seen in two urban health settings: a cholera treatment center (ctc) and oral rehydration points (orps). during april 1, 2011-november 30, 2012, stool samples were collected from 1,206 of 10,845 patients who came to the gheskio ctc or to the community orps with acute diarrhea, cultured for vibrio cholerae, and tested by multiplex polymerase reaction. vibrio cholerae was ... | 2014 | 24445205 |
| efficacy of zinc as an antibacterial agent against enteric bacterial pathogens. | diarrhoea is a serious threat all over the world with great economic implications especially evident in the developing world. this study was aimed at determining in vitro efficacy of zinc (zn) against common enteric bacterial pathogens. | 2014 | 24800334 |
| alkaloids: an overview of their antibacterial, antibiotic-enhancing and antivirulence activities. | with reports of pandrug-resistant bacteria causing untreatable infections, the need for new antibacterial therapies is more pressing than ever. alkaloids are a large and structurally diverse group of compounds that have served as scaffolds for important antibacterial drugs such as metronidazole and the quinolones. in this review, we highlight other alkaloids with development potential. natural, semisynthetic and synthetic alkaloids of all classes are considered, looking first at those with direc ... | 2014 | 25130096 |
| cholera outbreaks in africa. | during the current seventh cholera pandemic, africa bore the major brunt of global disease burden. more than 40 years after its resurgence in africa in 1970, cholera remains a grave public health problem, characterized by large disease burden, frequent outbreaks, persistent endemicity, and high cfrs, particularly in the region of the central african great lakes which might act as reservoirs for cholera. there, cases occur year round with a rise in incidence during the rainy season. elsewhere in ... | 2014 | 24827501 |
| bacterial periplasmic sialic acid-binding proteins exhibit a conserved binding site. | sialic acids are a family of related nine-carbon sugar acids that play important roles in both eukaryotes and prokaryotes. these sialic acids are incorporated/decorated onto lipooligosaccharides as terminal sugars in multiple bacteria to evade the host immune system. many pathogenic bacteria scavenge sialic acids from their host and use them for molecular mimicry. the first step of this process is the transport of sialic acid to the cytoplasm, which often takes place using a tripartite atp-indep ... | 2014 | 25004958 |
| association between giardia duodenalis and coinfection with other diarrhea-causing pathogens in india. | giardia duodenalis, is often seen as an opportunistic pathogen and one of the major food and waterborne parasites. some insights of giardia infestation in a diarrhoea-prone population were investigated in the present study. our primary goal was to understand the interaction of this parasite with other pathogens during infection and to determine some important factors regulating the diarrhoeal disease spectrum of a population. giardia showed a steady rate of occurrence throughout the entire study ... | 2014 | 25009820 |
| regulatory elements involved in the expression of competence genes in naturally transformable vibrio cholerae. | the human pathogen vibrio cholerae normally enters the developmental program of natural competence for transformation after colonizing chitinous surfaces. natural competence is regulated by at least three pathways in this organism: chitin sensing/degradation, quorum sensing and carbon catabolite repression (ccr). the cyclic adenosine monophosphate (camp) receptor protein crp, which is the global regulator of ccr, binds to regulatory dna elements called crp sites when in complex with camp. previo ... | 2014 | 25539806 |
| artist: high-resolution genome-wide assessment of fitness using transposon-insertion sequencing. | transposon-insertion sequencing (tis) is a powerful approach for deciphering genetic requirements for bacterial growth in different conditions, as it enables simultaneous genome-wide analysis of the fitness of thousands of mutants. however, current methods for comparative analysis of tis data do not adjust for stochastic experimental variation between datasets and are limited to interrogation of annotated genomic elements. here, we present artist, an accessible tis analysis pipeline for identify ... | 2014 | 25375795 |
| antibacterial and antioxidant activities in extracts of fully grown cladodes of 8 cultivars of cactus pear. | the antimicrobial and antioxidant activities of some cultivars of the nopal cactus have not been determined. in this study, 8 cultivars of nopal cacti from mexico were assayed for phenolic content, antioxidant activities, and antimicrobial activities against campylobacter jejuni, vibrio cholera, and clostridium perfringens. plant material was washed, dried, and macerated in methanol. minimum bactericidal concentrations (mbcs) were determined using the broth microdilution method. antioxidant acti ... | 2014 | 24621296 |
| establishing a list of qualifying pathogens under the food and drug administration safety and innovation act. final rule. | the food and drug administration (fda or agency) is issuing a regulation to establish a list of "qualifying pathogens'' that have the potential to pose a serious threat to public health. this final rule implements a provision of the generating antibiotic incentives now (gain) title of the food and drug administration safety and innovation act (fdasia). gain is intended to encourage development of new antibacterial and antifungal drugs for the treatment of serious or life-threatening infections, ... | 2014 | 24908687 |
| development and assessment of molecular diagnostic tests for 15 enteropathogens causing childhood diarrhoea: a multicentre study. | childhood diarrhoea can be caused by many pathogens that are difficult to assay in the laboratory. molecular diagnostic techniques provide a uniform method to detect and quantify candidate enteropathogens. we aimed to develop and assess molecular tests for identification of enteropathogens and their association with disease. | 2014 | 25022434 |
| biosynthesis, characterization and antimicrobial activity of silver nanoparticles by streptomyces sp. ss2. | in the present study the microbial biosynthesis of silver nanoparticles (agnps) by secondary metabolites of streptomyces sp. ss2 in an eco-friendly approach has been reported. the streptomyces sp. ss2 was isolated from the soil sediment of similipal biosphere reserve. the identification of this strain was determined by phenotypical characteristics (morphological and biochemical) and molecular characterization method using 16 s rdna sequencing. the morphological study was also done by high-resolu ... | 2014 | 24842223 |
| enzymatic sequencing of partially acetylated chitosan oligomers. | chitosan oligosaccharides have diverse biological activities with potentially valuable applications, for example, in the fields of medicine and agriculture. these functionalities are thought to depend on their degree of polymerization and acetylation, and possibly on specific patterns of acetylation. chitosan oligomers with fully defined architecture are difficult to produce, and their complete analysis is demanding. analysis is typically done using ms or nmr, requiring access to expensive infra ... | 2014 | 24824785 |
| the preliminary assessment of anti-microbial activity of hplc separated components of kirkia wilmsii. | most communities in developing countries rely on traditional medicines for the treatment of diseases. in south africa, the limpopo province, within the lebowakgomo district, uses tuberous roots of kirkia wilmsii, after infusion in water for the treatment of a wide range of diseases by sotho communities. | 2014 | 25371593 |
| serological surveillance development for tropical infectious diseases using simultaneous microsphere-based multiplex assays and finite mixture models. | a strategy to combat infectious diseases, including neglected tropical diseases (ntds), will depend on the development of reliable epidemiological surveillance methods. to establish a simple and practical seroprevalence detection system, we developed a microsphere-based multiplex immunoassay system and evaluated utility using samples obtained in kenya. | 2014 | 25078404 |
| atomic resolution crystal structure of vclmwptp-1 from vibrio cholerae o395: insights into a novel mode of dimerization in the low molecular weight protein tyrosine phosphatase family. | low molecular weight protein tyrosine phosphatase (lmwptp) is a group of phosphotyrosine phosphatase ubiquitously found in a wide range of organisms ranging from bacteria to mammals. dimerization in the lmwptp family has been reported earlier which follows a common mechanism involving active site residues leading to an enzymatically inactive species. here we report a novel form of dimerization in a lmwptp from vibrio cholera 0395 (vclmwptp-1). studies in solution reveal the existence of the dime ... | 2014 | 24909685 |
| antimicrobial activity, acute toxicity and cytoprotective effect of crassocephalum vitellinum (benth.) s. moore extract in a rat ethanol-hcl gastric ulcer model. | a decoction of crassocephallum vitellinum (benth.) s. moore (asteraceae) is used in kagera region to treat peptic ulcers. this study seeks to evaluate an aqueous ethanol extract of aerial parts of the plant for safety and efficacy. | 2014 | 24552147 |
| challenges in mucosal vaccines for the control of infectious diseases. | the mucosal surface is the largest route through which pathogens enter the human body. to control the outbreak of mucosal infectious diseases, we must use our knowledge of the mucosal immune system to create vaccines that elicit protective mucosal and systemic immunity. mucosal vaccines have advantages over traditional injectable vaccines in that they not only induce effective mucosal immune responses, but they also do not cause physical or psychological discomfort. mucosal vaccines currently li ... | 2014 | 24914172 |
| synergistic antimicrobial profiling of violacein with commercial antibiotics against pathogenic micro-organisms. | chromobacterium violaceum bergonzini (neisseriaceae), a gram-negative bacterium, secretes a spectacular pigment called violacein. violacein is a quorum-sensing metabolite and is also an active antimicrobial, anticancer agent. however, its efficiency as a potential drug, alone or in synergy with other active principles, has not being completely exploited. with the advent of different multi-drug resistant strains, it becomes essential to find a new natural product(s) that could be effectively used ... | 2014 | 24073823 |
| added value of multiplex luminex gastrointestinal pathogen panel (xtag® gpp) testing in the diagnosis of infectious gastroenteritis. | the luminex gastrointestinal pathogen panel (xtag(®) gpp) detects in one assay the most common gastroenteritis-causing pathogens and toxins, namely adenovirus 40/41, norovirus genogroup (ng) i/ii, rotavirus a, clostridium difficile toxin a/b, campylobacter sp., escherichia coli o157, enterotoxigenic e. coli heat-labile enterotoxin/heat-stable enterotoxin, salmonella sp., shiga-toxin producing e. coli, shiga-like toxin (stx)1/2, shigella sp., vibrio cholerae, yersinia enterocolitica, cryptosporid ... | 2014 | 24131399 |
| b-cell epitope of beta toxin of clostridium perfringens genetically conjugated to a carrier protein: expression, purification and characterization of the chimeric protein. | beta toxin (btx) is the prime virulence factor for the pathogenesis of clostridium perfringens type c strain, known to cause necrotic enteritis and enterotoxaemia in mammalian species. the existing vaccines targeting btx are formaldehyde inactivated culture filtrates of clostridium. these filtrates raise antigenic load in the host leading to nonspecific and poor responses. the present study aimed to overcome these drawbacks and generate a chimeric protein carrying in silico identified b-cell epi ... | 2014 | 24996028 |
| purification, structural elucidation and bioactivity of tryptophan containing diketopiperazines, from comamonas testosteroni associated with a rhabditid entomopathogenic nematode against major human-pathogenic bacteria. | the cell free culture filtrate of a comamonas testosteroni associated with an entomopathogenic nematode (epn), rhabditis (oscheius) sp. exhibited promising antimicrobial activity. the ethyl acetate extract of the bacterial culture filtrate was purified by silica gel column chromatography to obtain five diketopiperazines or cyclic dipeptides (dkp 1-5). the structure and absolute stereochemistry of the compounds were determined based on extensive spectroscopic analyses (hr-ms, (1)hnmr, (13)cnmr, ( ... | 2014 | 24120705 |
| stress tolerant virulent strains of cronobacter sakazakii from food. | cronobacter sakazakii is considered as an emerging foodborne pathogen. the aim of this study was to isolate and characterize virulent strains of cronobacter sakazakii from food samples of bangladesh. | 2014 | 25723712 |
| vibrio metoecus sp. nov., a close relative of vibrio cholerae isolated from coastal brackish ponds and clinical specimens. | a gram-staining-negative, curved-rod-shaped bacterium with close resemblance to vibrio cholerae, the aetiological agent of cholera, was isolated over the course of several years from coastal brackish water (17 strains) and from clinical cases (two strains) in the united states. 16s rrna gene identity with v. cholerae exceeded 98 % yet an average nucleotide identity based on genome data of around 86 % and multi locus sequence analysis of six housekeeping genes (mdh, adk, gyrb, reca, pgi and rpob) ... | 2014 | 24972615 |
| recombinant toxin-coregulated pilus a (tcpa) as a candidate subunit cholera vaccine. | the toxin co-regulated pilus a (tcpa) has been described as a critical pathogenicity factor of vibrio cholerae. tcpa is a candidate for making subunit vaccine against cholera. the aim of this study was to produce a candidate vaccine by expressing recombinant tcpa in e. coli. | 2014 | 25705354 |
| ypfφ: a filamentous phage acquired by yersinia pestis. | yersinia pestis, the plague bacillus, has an exceptional pathogenicity for humans. the plague bacillus emerged very recently (≈3,000 years ago) from the enteropathogen y. pseudotuberculosis. early after its emergence, y. pestis became infected by a filamentous phage named ypfφ. during the microevolution of the plague bacillus, the phage remained in the various lineages as an unstable extrachromosomal element. however, in the sub branch that caused the third plague pandemic, ypfφ integrated itsel ... | 2014 | 25566217 |
| novel bacterial adp-ribosylating toxins: structure and function. | bacterial adp-ribosyltransferase toxins (bartts) transfer adp-ribose to eukaryotic proteins to promote bacterial pathogenesis. in this review, we use prototype bartts, such as diphtheria toxin and pertussis toxin, as references for the characterization of several new bartts from human, insect and plant pathogens, which were recently identified by bioinformatic analyses. several of these toxins, including cholix toxin (chxa) from vibrio cholerae, spya from streptococcus pyogenes, hopu1 from pseud ... | 2014 | 25023120 |
| assembly and stability of salmonella enterica ser. typhi tolc protein in pope and dmpe. | in this work we assessed the suitability of two different lipid membranes for the simulation of a tolc protein from salmonella enterica serovar typhi. the tolc protein family is found in many pathogenic gram-negative bacteria including vibrio cholera and pseudomonas aeruginosa and acts as an outer membrane channel for expulsion of drug and toxin from the cell. in s. typhi, the causative agent for typhoid fever, the tolc outer membrane protein is an antigen for the pathogen. the lipid environment ... | 2014 | 25011632 |
| biosynthesis of silver nanoparticles from deep sea bacterium pseudomonas aeruginosa jq989348 for antimicrobial, antibiofilm, and cytotoxic activity. | pseudomonas aeruginosa (jq989348) was isolated from deep sea water sample and used for synthesis of silver nanoparticles (agnps). agnps were confirmed by analyzing surface plasmon resonance using uv-visible spectrophotometer at 420 nm. further scanning electron microscope analysis confirmed the range of particle size between 13 and 76 nm and xrd pattern authorizes the anisotropic crystalline nature of agnps. fourier transform infrared spectrum endorsed the presence of high amount of proteins and ... | 2014 | 24136453 |
| an optofluidic imaging system to measure the biophysical signature of single waterborne bacteria. | in this paper, for the first time, an on-chip optofluidic imaging system is innovated to measure the biophysical signatures of single waterborne bacteria, including both their refractive indices and morphologies (size and shape), based on immersion refractometry. the key features of the proposed optofluidic imaging platform include (1) multiple sites for single-bacterium trapping, which enable parallel measurements to achieve higher throughput, and (2) a chaotic micromixer, which enables efficie ... | 2014 | 25205636 |
| exploring insights for virulent gene inhibition of multidrug resistant salmonella typhi, vibrio cholerae, and staphylococcus areus by potential phytoligands via in silico screening. | in our recent studies on prevalence of multidrug resistant pathogens in byramangala reservoir, karnataka, india, we identified salmonella typhi, staphylococcus aureus, and vibrio cholerae which had acquired multiple drug resistance (mdr) and emerged as superbugs. hence, there is a pressing demand to identify alternative therapeutic remedies. our study focused on the screening of herbal leads by structure-based virtual screening. the virulent gene products of these pathogens towards kanamycin(aph ... | 2014 | 23876154 |
| biotechnological potential of the seaweed cladophora rupestris (chlorophyta, cladophorales) lipidic extract. | recently, with the advent of modern technologies, various marine organisms including algae are being studied as sources of natural substances effective on classical microorganisms and able to also combat the new trend of acquired resistance in microbes. in the present study the antimicrobial activity of the lipidic extract of the green seaweed cladophora rupestris collected in a mediterranean area, in two sampling periods (january and april), was assayed. the chemical characterization of the lip ... | 2014 | 24852224 |
| multiplex genome editing by natural transformation. | editing bacterial genomes is an essential tool in research and synthetic biology applications. here, we describe multiplex genome editing by natural transformation (mugent), a method for accelerated evolution based on the cotransformation of unlinked genetic markers in naturally competent microorganisms. we found that natural cotransformation allows scarless genome editing at unprecedented frequencies of ∼50%. using dna substrates with randomized nucleotides, we found no evidence for bias during ... | 2014 | 24889608 |
| genome-wide fitness and genetic interactions determined by tn-seq, a high-throughput massively parallel sequencing method for microorganisms. | the lagging annotation of bacterial genomes and the inherent genetic complexity of many phenotypes is hindering the discovery of new drug targets and the development of new antimicrobial agents and vaccines. this unit presents tn-seq, a method that has made it possible to quantitatively determine fitness for most genes in a microorganism and to screen for quantitative genetic interactions on a genome-wide scale and in a high-throughput fashion. tn-seq can thus direct studies on the annotation of ... | 2014 | 24733243 |
| occurrence of virulence genes among vibrio cholerae and vibrio parahaemolyticus strains from treated wastewaters. | pathogenic vibrio species are an important cause of foodborne illnesses. the aim of this study was to describe the occurrence of potentially pathogenic vibrio species in the final effluents of a wastewater treatment plant and the risk that they may pose to public health. during the 1-year monitoring, a total of 43 vibrio strains were isolated: 23 vibrio alginolyticus, 1 vibrio cholerae, 4 vibrio vulnificus, and 15 vibrio parahaemolyticus. the pcr investigation of v. parahaemolyticus and v. chole ... | 2014 | 25023745 |
| multiplex pcr assays for the detection of vibrio alginolyticus, vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae with an internal amplification control. | a multiplex polymerase chain reaction (pcr) assay that can simultaneously detect 4 major vibrio spp., vibrio alginolyticus, vibrio parahaemolyticus, vibrio vulnificus, and vibrio cholerae, in the presence of an internal amplification control (iac) was developed. species-specific pcr primers were designed based on the gyrb gene for v. alginolyticus, the collagenase gene for v. parahaemolyticus, the vvha gene for v. vulnificus, and the ompw gene for v. cholerae. additionally, an iac primer pair wa ... | 2014 | 24731836 |
| the cholera outbreak in haiti: where and how did it begin? | in october 2010, cholera appeared in haiti for the first time in nearly a century. the secretary-general of the united nations formed an independent panel to "investigate and seek to determine the source of the 2010 cholera outbreak in haiti". to fulfill this mandate, the panel conducted concurrent epidemiological, water and sanitation, and molecular analysis investigations. our may 2011 findings indicated that the 2010 haiti cholera outbreak was caused by bacteria introduced into haiti as a res ... | 2014 | 23695726 |
| repeated dose toxicity study of vibrio cholerae-loaded gastro-resistant microparticles. | microencapsulation of antigens has been extensively studied over the last decades aiming at improving the immunogenicity of vaccine candidates. | 2014 | 23795905 |
| insights on na(+) binding and conformational dynamics in multidrug and toxic compound extrusion transporter norm. | mate (multidrug and toxic compound extrusion) transporter proteins mediate metabolite transport in plants and multidrug resistance in bacteria and mammals. mate transporter norm from vibrio cholerae is an antiporter that is driven by na+ gradient to extrude the substrates. to understand the molecular mechanism of na+-substrate exchange, molecular dynamics simulation was performed to study conformational changes of both wild-type and mutant norm with and without cation bindings. our results show ... | 2014 | 23873591 |
| haitian variant ctxb producing vibrio cholerae o1 with reduced susceptibility to ciprofloxacin is persistent in yavatmal, maharashtra, india, after causing a cholera outbreak. | vibrio cholerae o1 biotype el tor producing haitian variant cholera toxin (hct) and showing reduced susceptibility to ciprofloxacin caused a cholera outbreak associated with a high case fatality rate (4.5) in india. hct-secreting strains responsible for severe cholera epidemics in orissa (india), western africa and haiti were associated with increased mortality. there is a pressing need for an integrated multidisciplinary approach to combat further spread of newly emerging variant strains. the t ... | 2014 | 24102849 |
| cryo-electron microscopy reveals the membrane insertion mechanism of v. cholerae hemolysin. | vibrio cholerae hemolysin (hlya) is a 65 kda pore-forming toxin which causes lysis of target eukaryotic cells by forming heptameric channels in the plasma membrane. deletion of the 15 kda c-terminus β-prism carbohydrate-binding domain generates a 50 kda truncated variant (hlya50) with 1000-fold-reduced pore-forming activity. previously, we showed by cryo-electron microscopy that the two toxin oligomers have central channels, but the 65 kda toxin oligomer is a seven-fold symmetric structure with ... | 2014 | 24102290 |
| detection of vibrio parahaemolyticus, vibrio vulnificus and vibrio cholerae with respect to seasonal fluctuations in temperature and plankton abundance. | over a 1-year period, bi-monthly estuarine surface water and plankton samples (63-200 and > 200 μm fractions) were assayed by polymerase chain reaction for the prevalence of total vibrio parahaemolyticus, v. vulnificus and v. cholerae and select genes associated with clinical strains found in each species. neither temperature nor plankton abundance was a significant correlate of total v. parahaemolyticus; however, the prevalence of genes commonly associated with clinical strains (trh, tdh, orf8) ... | 2014 | 24024909 |
| a novel 8-nm protein cage formed by vibrio cholerae acylphosphatase. | here we show the formation of an ~8-nm cage formed by the self-assembly of acylphosphatase from vibrio cholerae o395 (vc-acp). the 12-subunit cage structure forms spontaneously and is stabilized through binding of sulfate ions at its exterior face and interfacial regions. crystal structure and studies in solutions illuminate the basis for the formation of the cage, while a single (cys20→arg) mutation (vc-acp-c20r) transforms vc-acp to a potent enzyme but disrupts the assembly into a trimer. | 2014 | 24055378 |
| the greatest steps towards the discovery of vibrio cholerae. | in the 19th century, there was extensive research on cholera: the disease was generally attributed to miasmatic causes, but this concept was replaced, between about 1850 and 1910, by the scientifically founded germ theory of disease. in 1883, robert koch identified the vibrion for the second time, after filippo pacini's discovery in 1854: koch isolated the comma bacillus in pure culture and explained its mode of transmission, solving an enigma that had lasted for centuries. the aim of this artic ... | 2014 | 24191858 |
| resveratrol--a potential inhibitor of biofilm formation in vibrio cholerae. | resveratrol, a phytochemical commonly found in the skin of grapes and berries, was tested for its biofilm inhibitory activity against vibrio cholerae. biofilm inhibition was assessed using crystal violet assay. mtt assay was performed to check the viability of the treated bacterial cells and the biofilm architecture was analysed using confocal laser scanning microscopy. the possible target of the compound was determined by docking analysis. results showed that subinhibitory concentrations of the ... | 2014 | 24182988 |
| characterization of vibrio cholerae from 1986 to 2012 in yunnan province, southwest china bordering myanmar. | vibrio cholerae is an important infectious pathogen causing serious human diarrhea. we analyzed 568 v. cholerae strains isolated from 1986 to 2012 in yunnan province, southwest china bordering myanmar. polymerase chain reactions for detecting virulence genes, antibiotic susceptibility tests and pulse-field gel electrophoresis (pfge) were performed. the results showed all the strains were el tor biotype from 1986. the ctxb subunit sequence analysis for all strains have shown that cholera between ... | 2014 | 24177595 |
| safety and immunogenicity of single-dose live oral cholera vaccine strain cvd 103-hgr, prepared from new master and working cell banks. | currently, no cholera vaccine is available for persons traveling from the united states to areas of high cholera transmission and who for reasons of occupation or host factors are at increased risk for development of the disease. a single-dose oral cholera vaccine with a rapid onset of protection would be particularly useful for such travelers and might also be an adjunct control measure for cholera outbreaks. the attenuated vibrio cholerae o1 vaccine strain cvd 103-hgr harbors a 94% deletion of ... | 2014 | 24173028 |
| emergence of vibrio cholerae o1 classical biotype in 2012 in iran. | cholera outbreaks annually occur in many parts of iran. the aim of this study was to investigate the biotype and genotype diversity of v. cholerae isolates from recent outbreak (2012) in iran and to characterize the ctxb allelic sequence of isolates. the ctxb sequence of all isolates was analyzed and compared with the reference ctxb sequences for el tor and classical biotypes in genbank database. the pfge genotype specification of isolates was determined and genetic relatedness among isolates an ... | 2014 | 24117836 |
| sodium-coupled dicarboxylate and citrate transporters from the slc13 family. | the slc13 family in humans and other mammals consists of sodium-coupled transporters for anionic substrates: three transporters for dicarboxylates/citrate and two transporters for sulfate. this review will focus on the di- and tricarboxylate transporters: nadc1 (slc13a2), nadc3 (slc13a3), and nact (slc13a5). the substrates of these transporters are metabolic intermediates of the citric acid cycle, including citrate, succinate, and α-ketoglutarate, which can exert signaling effects through specif ... | 2014 | 24114175 |
| genetic variation of vibrio cholerae during outbreaks, bangladesh, 2010-2011. | cholera remains a major public health problem. to compare the relative contribution of strains from the environment with strains isolated from patients during outbreaks, we performed multilocus variable tandem repeat analyses on samples collected during the 2010 and 2011 outbreak seasons in 2 geographically distinct areas of bangladesh. a total of 222 environmental and clinical isolates of v. cholerae o1 were systematically collected from chhatak and mathbaria. in chhatak, 75 of 79 isolates were ... | 2014 | 24377372 |
| zebrafish as a natural host model for vibrio cholerae colonization and transmission. | the human diarrheal disease cholera is caused by the aquatic bacterium vibrio cholerae. v. cholerae in the environment is associated with several varieties of aquatic life, including insect egg masses, shellfish, and vertebrate fish. here we describe a novel animal model for v. cholerae, the zebrafish. pandemic v. cholerae strains specifically colonize the zebrafish intestinal tract after exposure in water with no manipulation of the animal required. colonization occurs in close contact with the ... | 2014 | 24375135 |
| cqsa-cqss quorum-sensing signal-receptor specificity in photobacterium angustum. | quorum sensing (qs) is a process of bacterial cell-cell communication that relies on the production, detection and population-wide response to extracellular signal molecules called autoinducers. the qs system commonly found in vibrios and photobacteria consists of the cqsa synthase/cqss receptor pair. vibrio cholerae cqsa/s synthesizes and detects (s)-3-hydroxytridecan-4-one (c10-cai-1), whereas vibrio harveyi produces and detects a distinct but similar molecule, (z)-3-aminoundec-2-en-4-one (ea- ... | 2014 | 24372841 |
| [antibiotic susceptibility of vibrio cholerae non o1/non o139 serogroups isolated from environment in the rostov region]. | analysis of the antibioticograms of 22 strains of vibrio cholerae non o1/non o139 serogroups (ctxa- tepa-) isolated from the environment in the rostov region in 2011 showed that all the cultures were susceptible to ciprofloxacin, aminoglycosides, ceftriaxone, trimetoprime/sulfamethoxazole and resistant to levomycetin and furazolidone. 32%, 18% and 9% of the isolates were resistant to tetracycline, rifampicin and nalidixic acid respectively. no strains of v. cholerae susceptible to all the tested ... | 2014 | 26448988 |
| haitian variant tcpa in vibrio cholerae o1 el tor strains in kolkata, india. | 2014 | 24371245 | |
| cholera outbreaks in the classical biotype era. | in the indian subcontinent description of a disease resembling cholera has been mentioned in sushruta samita, estimated to have been written between ~400 and 500 bc. it is however not clear whether the disease known today as cholera caused by vibrio cholerae vibrio cholerae o1 is the evolutionary progression of the ancient disease. the modern history of cholera began in 1817 when an explosive epidemic broke out in the ganges river delta region of bengal. this was the first of the seven recorded ... | 2014 | 24368696 |
| comparative genomics study for identification of drug and vaccine targets in vibrio cholerae: mura ligase as a case study. | a systematic workflow consisting of comparative genomics, metabolic pathways analysis and additional drug prioritization parameters identified 264 proteins of vibrio cholerae which were predicted to be absent in homo sapiens. among these, 40 proteins were identified as essential proteins that could serve as potential drug and vaccine targets. additional prioritization parameters characterized 11 proteins as vaccine candidates while druggability of each of the identified proteins as evaluated by ... | 2014 | 24368230 |
| pre-earthquake non-epidemic vibrio cholerae in haiti. | to our knowledge, there was no record of vibrio cholerae in haiti until the 2010 post earthquake outbreak. | 2014 | 24423722 |
| crystallization and preliminary x-ray study of vibrio cholerae uridine phosphorylase in complex with 6-methyluracil. | uridine phosphorylase catalyzes the phosphorolysis of ribonucleosides, with the nitrogenous base and ribose 1-phosphate as products. additionally, it catalyzes the reverse reaction of the synthesis of ribonucleosides from ribose 1-phosphate and a nitrogenous base. however, the enzyme does not catalyze the synthesis of nucleosides when the substrate is a nitrogenous base substituted at the 6-position, such as 6-methyluracil (6-mu). in order to explain this fact, it is essential to investigate the ... | 2014 | 24419619 |
| circulation and transmission of clones of vibrio cholerae during cholera outbreaks. | cholera is still a major public health problem. the underlying bacterial pathogen vibrio cholerae (v. cholerae) is evolving and some of its mutations have set the stage for outbreaks. after v. cholerae acquired the mobile elements vsp i & ii, the el tor pandemic began and spread across the tropics. the replacement of the o1 serotype encoding genes with the o139 encoding genes triggered an outbreak that swept across the indian subcontinent. the sxt element generated a third selective sweep and mo ... | 2014 | 24407776 |
| evaluation of enrichment method for the detection of vibrio cholerae o1 using a rapid dipstick test in bangladesh. | to evaluate the specificity of the crystal vc dipstick test for detecting cholera. | 2014 | 24401137 |
| second-pandemic strain of vibrio cholerae from the philadelphia cholera outbreak of 1849. | in the 19th century, there were several major cholera pandemics in the indian subcontinent, europe, and north america. the causes of these outbreaks and the genomic strain identities remain a mystery. we used targeted high-throughput sequencing to reconstruct the vibrio cholerae genome from the preserved intestine of a victim of the 1849 cholera outbreak in philadelphia, part of the second cholera pandemic. this o1 biotype strain has 95 to 97% similarity with the classical o395 genome, differing ... | 2014 | 24401020 |
| prevalence of vibrio cholerae o1 el tor variant in a cholera-endemic zone of kenya. | since 2007, kenya has experienced an increase in cholera outbreaks characterized by a high fatality rate. in this study, we characterized 81 vibrio cholerae isolates from diarrhoeal stool samples in nyanza, a cholera-endemic lake region of kenya, for virulence properties, clonality and antibiotic susceptibility. eighty of these isolates were v. cholerae o1 el tor variants carrying the classical ctxb gene sequence, while one isolate was v. cholerae non-o1/o139. all of the el tor variants were of ... | 2014 | 24396087 |
| development of an immunochromatographic lateral flow device for rapid diagnosis of vibrio cholerae o1 serotype ogawa. | cholera is an acute malignant infectious disease caused by the bacteria vibrio cholerae leading to severe dehydrating diarrhea and vomiting, even high rates of mortality in some cases. however, the prevention of the epidemic disease is achievable if proper sanitation practices are followed, provided the accurate and prompt diagnosis of each prevalent serotype in cholera epidemic. the current gold standard of bacterial culture is inadequate for rapid diagnosis. our aim is to develop an immunochro ... | 2014 | 24389075 |
| construction and evaluation of v. cholerae o139 mutant, vcusm21p, as a safe live attenuated cholera vaccine. | cholera is a major infectious disease, affecting millions of lives annually. in endemic areas, implementation of vaccination strategy against cholera is vital. as the use of safer live vaccine that can induce protective immunity against vibrio cholerae o139 infection is a promising approach for immunization, we have designed vcusm21p, an oral cholera vaccine candidate, which has ctxa that encodes a subunit of ctx and mutated rtxa/c, ace and zot mutations. vcusm21p was found not to disassemble th ... | 2014 | 24505241 |
| high-salt preadaptation of vibrio parahaemolyticus enhances survival in response to lethal environmental stresses. | adaptation to changing environmental conditions is an important strategy for survival of foodborne bacterial pathogens. vibrio parahaemolyticus is a gram-negative seafoodborne enteric pathogen found in the marine environment both free living and associated with oysters. this pathogen is a moderate halophile, with optimal growth at 3% nacl. among the several stresses imposed upon enteric bacteria, acid stress is perhaps one of the most important. v. parahaemolyticus has a lysine decarboxylase sys ... | 2014 | 24490918 |
| cholera epidemic associated with consumption of unsafe drinking water and street-vended water--eastern freetown, sierra leone, 2012. | during 2012, sierra leone experienced a cholera epidemic with 22,815 reported cases and 296 deaths. we conducted a matched case-control study to assess risk factors, enrolling 49 cases and 98 controls. stool specimens were analyzed by culture, polymerase chain reaction (pcr), and pulsed-field gel electrophoresis (pfge). conditional logistic regression found that consuming unsafe water (matched odds ratio [mor]: 3.4; 95% confidence interval [ci]: 1.1, 11.0), street-vended water (mor: 9.4; 95% ci: ... | 2014 | 24470563 |
| dynamics in genome evolution of vibrio cholerae. | vibrio cholerae, the etiological agent of the acute secretary diarrheal disease cholera, is still a major public health concern in developing countries. in former centuries cholera was a permanent threat even to the highly developed populations of europe, north america, and the northern part of asia. extensive studies on the cholera bug over more than a century have made significant advances in our understanding of the disease and ways of treating patients. v. cholerae has more than 200 serogrou ... | 2014 | 24462909 |
| functional and structural characterization of vibrio cholerae extracellular serine protease b, vesb. | the chymotrypsin subfamily a of serine proteases consists primarily of eukaryotic proteases, including only a few proteases of bacterial origin. vesb, a newly identified serine protease that is secreted by the type ii secretion system in vibrio cholerae, belongs to this subfamily. vesb is likely produced as a zymogen because sequence alignment with trypsinogen identified a putative cleavage site for activation and a catalytic triad, his-asp-ser. using synthetic peptides, vesb efficiently cleaved ... | 2014 | 24459146 |
| small rnas in the vibrionaceae: an ocean still to be explored. | in bacteria, the discovery of noncoding small rnas (srnas) as modulators of gene expression in response to environmental signals has brought new insights into bacterial gene regulation, including control of pathogenicity. the vibrionaceae constitute a family of marine bacteria of which many are responsible for infections affecting not only humans, such as vibrio cholerae but also fish and marine invertebrates, representing the major cause of mortality in farmed marine species. they are able to c ... | 2014 | 24458378 |
| long-term comparison of antibiotic resistance in vibrio cholerae o1 and shigella species between urban and rural bangladesh. | from 2000 to 2012, vibrio cholerae o1 and shigella species isolates from urban dhaka and rural matlab were tested for resistance to all clinically relevant antibiotics in bangladesh. resistances in urban and rural bangladesh tended to rise and fall together, especially a few years after the introduction of new resistance. | 2014 | 24457344 |
| isolation and characterization of lytic vibriophage against vibrio cholerae o1 from environmental water samples in kelantan, malaysia. | water samples from a variety of sources in kelantan, malaysia (lakes, ponds, rivers, ditches, fish farms, and sewage) were screened for the presence of bacteriophages infecting vibrio cholerae. ten strains of v. cholerae that appeared to be free of inducible prophages were used as the host strains. eleven bacteriophage isolates were obtained by plaque assay, three of which were lytic and further characterized. the morphologies of the three lytic phages were similar with each having an icosahedra ... | 2014 | 24532381 |
| structural characterization of the extracellular polysaccharide from vibrio cholerae o1 el-tor. | the ability to form biofilms is important for environmental survival, transmission, and infectivity of vibrio cholerae, the causative agent of cholera in humans. to form biofilms, v. cholerae produces an extracellular matrix composed of proteins, nucleic acids and a glycoconjugate, termed vibrio exopolysaccharide (vps). here, we present the data on isolation and characterization of the polysaccharide part of the vps (vps-ps), which has the following structure: -4)-α-gulpnacagly3oac-(1-4)-β-d-glc ... | 2014 | 24520310 |
| genome sequence and comparative analysis of a vibrio cholerae o139 strain e306 isolated from a cholera case in china. | vibrio cholerae is a human intestinal pathogen and v. cholerae of the o139 serogroups are responsible for the current epidemic cholera in china. in this work, we reported the whole genome sequencing of a v. cholerae o139 strain e306 isolated from a cholera patient in the 306th hospital of pla, beijing, china. | 2014 | 24517211 |
| draft genome sequence of environmental vibrio cholerae 2012el-1759 with similarities to the v. cholerae o1 classical biotype. | vibrio cholerae 2012el-1759 is an environmental isolate from haiti that was recovered in 2012 during a cholera outbreak. the genomic backbone is similar to that of the prototypical v. cholerae o1 classical biotype strain o395, and it carries the vibrio pathogenicity islands (vpi-1 and vpi-2) and a cholera toxin (ctx) prephage. | 2014 | 25013135 |
| cyclo(valine-valine) inhibits vibrio cholerae virulence gene expression. | vibrio cholerae has been shown to produce a cyclic dipeptide, cyclo(phenylalanine-proline) (cfp), that functions to repress virulence factor production. the objective of this study was to determine if heterologous cyclic dipeptides could repress v. cholerae virulence factor production. to that end, three synthetic cyclic dipeptides that differed in their side chains from cfp were assayed for virulence inhibitory activity in v. cholerae. the results revealed that cyclo(valine-valine) (cvv) inhibi ... | 2014 | 24644247 |
| a novel and simple mixture as point-of-use water treatment agent to produce safe drinking water. | people in rural bangladesh have a poor understanding of the link between use of contaminated surface water and disease. an inexpensive point-of-use water treatment agent was developed to purify surface water. | 2014 | 24619586 |
| the 1.59å resolution structure of the minor pseudopilin epsh of vibrio cholerae reveals a long flexible loop. | the type ii secretion complex exports folded proteins from the periplasm to the extracellular milieu. it is used by the pathogenic bacterium vibrio cholerae to export several proteins, including its major virulence factor, cholera toxin. the pseudopilus is an essential component of the type ii secretion system and likely acts as a piston to push the folded proteins across the outer membrane through the secretin pore. the pseudopilus is composed of the major pseudopilin, epsg, and four minor pseu ... | 2014 | 24316251 |
| fuse or die: how to survive the loss of dam in vibrio cholerae. | dam methylates gatc sequences in γ-proteobacteria genomes, regulating several cellular functions including replication. in vibrio cholerae, which has two chromosomes, dam is essential for viability, owing to its role in chr2 replication initiation. in this study, we isolated spontaneous mutants of v. cholerae that were able to survive the deletion of dam. in these mutants, homologous recombination and chromosome dimer resolution are essential, unless dna mismatch repair is inactivated. furthermo ... | 2014 | 24308271 |
| solutions to the public goods dilemma in bacterial biofilms. | bacteria frequently live in densely populated surface-bound communities, termed biofilms [1-4]. biofilm-dwelling cells rely on secretion of extracellular substances to construct their communities and to capture nutrients from the environment [5]. some secreted factors behave as cooperative public goods: they can be exploited by nonproducing cells [6-11]. the means by which public-good-producing bacteria avert exploitation in biofilm environments are largely unknown. using experiments with vibrio ... | 2014 | 24332540 |
| the luxr-type regulator vpst negatively controls the transcription of rpos, encoding the general stress response regulator, in vibrio cholerae biofilms. | cholera is a waterborne diarrheal disease caused by vibrio cholerae strains of serogroups o1 and o139. expression of the general stress response regulator rpos and formation of biofilm communities enhance the capacity of v. cholerae to persist in aquatic environments. the transition of v. cholerae between free-swimming (planktonic) and biofilm life-styles is regulated by the second messenger cyclic di-gmp (c-di-gmp). we previously reported that increasing the c-di-gmp pool by overexpression of a ... | 2014 | 24363348 |
| inhibition of the sodium-translocating nadh-ubiquinone oxidoreductase [na+-nqr] decreases cholera toxin production in vibrio cholerae o1 at the late exponential growth phase. | two virulence factors produced by vibrio cholerae, cholera toxin (ct) and toxin-corregulated pilus (tcp), are indispensable for cholera infection. toxt is the central regulatory protein involved in activation of ct and tcp expression. we previously reported that lack of a respiration-linked sodium-translocating nadh-ubiquinone oxidoreductase (na(+)-nqr) significantly increases toxt transcription. in this study, we further characterized this link and found that na(+)-nqr affects toxt expression o ... | 2014 | 24361395 |
| the β-prism lectin domain of vibrio cholerae hemolysin promotes self-assembly of the β-pore-forming toxin by a carbohydrate-independent mechanism. | vibrio cholerae cytolysin/hemolysin (vcc) is an amphipathic 65-kda β-pore-forming toxin with a c-terminal β-prism lectin domain. because deletion or point mutation of the lectin domain seriously compromises hemolytic activity, it is thought that carbohydrate-dependent interactions play a critical role in membrane targeting of vcc. to delineate the contributions of the cytolysin and lectin domains in pore formation, we used wild-type vcc, 50-kda vcc (vcc(50)) without the lectin domain, and mutant ... | 2014 | 24356964 |
| the seventh pandemic vibrio cholerae o1 el tor isolate in china has undergone genetic shifts. | a total of 330 clinical vibrio cholerae o1 serogroups from china dating between 1961 and 2010 were investigated. by phenotypic biotyping and genetic analysis, during the seventh pandemic of v. cholerae o1 in china, the isolates of hybrid biotype (mixed classical phenotypes) were present during the entire1961-2010 period, while el tor genetic shifts appeared in 1992 and replaced the prototype el tor from 2002 to 2010. | 2014 | 24353005 |
| a molecular surveillance reveals the prevalence of vibrio cholerae o139 isolates in china from 1993 to 2012. | vibrio cholerae serogroup o139 was first identified in 1992 in india and bangladesh, in association with major epidemics of cholera in both countries; cases were noted shortly thereafter in china. we characterized 211 v. cholerae o139 isolates that were isolated at multiple sites in china between 1993 and 2012 from patients (n = 92) and the environment (n = 119). among clinical isolates, 88 (95.7%) of 92 were toxigenic, compared with 47 (39.5%) of 119 environmental isolates. toxigenic isolates c ... | 2014 | 24452176 |
| population structural analysis of o1 el tor vibrio cholerae isolated in china among the seventh cholera pandemic on the basis of multilocus sequence typing and virulence gene profiles. | serogroup o1 vibrio cholerae is the most common agents to cause epidemic and pandemic cholera disease. in this study, multilocus sequence typing (mlst) was performed on 160 serogroup o1 strains (including 42 toxigenic and 118 non-toxigenic), and the virulence/fitness gene profiles of 16 loci were further analysed for 60 strains of these. eighty-four sequence types (sts) with 14 clonal complexes were distinguished, and 29 sts were unique. except sd19771005, all toxigenic strains were well-separat ... | 2014 | 24448269 |
| influence of climate factors on vibrio cholerae dynamics in the pearl river estuary, south china. | current research has seldom focused on the quantitative relationships between vibrio cholerae (v. cholerae) and climate factors owing to the complexities and high cost of field observation in the aquatic environment. this study has focused on the relationships between v. cholerae and climate factors based on linear regression method and data partition method. data gathered from 2008 to 2009 in the pearl river estuary, south china, were adopted. positive rate of v. cholerae was correlated closely ... | 2014 | 24442820 |
| highly potent, chemically stable quorum sensing agonists for vibrio cholerae. | in the vibrio cholerae pathogen, initiation of bacterial quorum sensing pathways serves to suppress virulence. we describe herein a potent and chemically stable small molecule agonist of v. cholerae quorum sensing, which was identified through rational drug design based on the native quorum sensing signal. this novel agonist may serve as a useful lead compound for the control of virulence in v. cholerae. | 2014 | 24436778 |
| genomic epidemiology of vibrio cholerae o1 associated with floods, pakistan, 2010. | in august 2010, pakistan experienced major floods and a subsequent cholera epidemic. to clarify the population dynamics and transmission of vibrio cholerae in pakistan, we sequenced the genomes of all v. cholerae o1 el tor isolates and compared the sequences to a global collection of 146 v. cholerae strains. within the global phylogeny, all isolates from pakistan formed 2 new subclades (psc-1 and psc-2), lying in the third transmission wave of the seventh-pandemic lineage that could be distingui ... | 2014 | 24378019 |