Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [the clinical and echographic assessment of thyroid function and structure in patients with a chronic inflammatory intestinal disease]. | in crohn's disease (cd) and in ulcerative colitis (uc) data about possible thyroid involvement are lacking. we studied thyroid morphology and function in 41 patients affected by active inflammatory bowel disease (ibd) (14 uc; 27 cd) before (all) and 45 and 90 days after onset of therapy (23/41), and in 60 normal control subjects. at each time, the following tests were performed: thyroid sonography, hormonal and immunological assays. a statistically significant increase of thyroid volume was foun ... | 1999 | 10193158 |
| thyroid hormone is essential for pituitary somatotropes and lactotropes. | mice homozygous for a disruption in the alpha-subunit essential for tsh, lh, and fsh activity (alphagsu-/-) exhibit hypothyroidism and hypogonadism similar to that observed in tsh receptor-deficient hypothyroid mice (hyt) and gnrh-deficient hypogonadal mutants (hpg). although the five major hormone-producing cells of the anterior pituitary are present in alphagsu-/- mice, the relative proportions of each cell type are altered dramatically. thyrotropes exhibit hypertrophy and hyperplasia, and som ... | 1999 | 10098528 |
| effect of ptu treatment on histone acetylation pattern in the developing rat brain. | the effect of hypothyroidism induced m female rats on histone acetylation pattern m the neonatal rat brain was studied. it is likely that thyroid hormone regulates the acetylation of histones and thereby influence their interaction with dna and modulates transcription. propylthiouracil (ptu), administered to induce hypothyroidism, resulted in a significant reduction m the thyroid and brain weight of neonatal rats. the circulating thyroxine levels were undetectable in both 14 and 21 day old pups. ... | 1999 | 10098595 |
| the relationship between submaximal activity of the lumbar extensor muscles and lumbar posteroanterior stiffness. | some patients with low back pain are thought to have increased lumbar posteroanterior (pa) stiffness. increased activity of the lumbar extensors could contribute to this stiffness. this activity may be seen when a pa force is applied and is thought to represent much less force than occurs with a maximal voluntary contraction (mvc). although mvcs of the lumbar extensors are known to increase lumbar pa stiffness, the effect of small amounts of voluntary contraction is not known. in this study, the ... | 1999 | 10078771 |
| [a thyroid function study in subjects with down's syndrome]. | the main objective is to know the application degree of spanish health program for down syndrome people respect to thyroid function. the secondary objective is to know the thyroid pathology in our population. | 1999 | 10081172 |
| anal submucosal injection: a novel modality for the treatment of advanced rectal cancer. | to investigate the efficacy of methotrexate (mtx) submucosal anal injection in the treatment of advanced rectal cancer. | 1999 | 10188860 |
| [iodine deficiency, iodine requirement and iodine excess of farm animals--experiments on growing pigs]. | since insufficient iodine intake is widespread among the german population, farm animals should if possible receive iodine above requirement, thus concentrating the trace element in milk, eggs and possibly meat. experiments with a total of 29 fattening pigs with grain soy-bean meal diets without iodine supplementation or with increasing supplements up to 1000 micrograms iodine/kg diet did not show any effect on growth intensity (gain) and feed efficiency. at and above 125 micrograms supplementar ... | 1999 | 10189724 |
| characteristics of recovery from the euthyroid sick syndrome induced by tumor necrosis factor alpha in cancer patients. | cytokines have been implicated in the pathogenesis of the euthyroid sick syndrome. isolated limb perfusion (ilp) with recombinant human tumor necrosis factor alpha (rtnf) and melphalan in patients with melanoma or sarcoma is accompanied by high systemic tnf levels. we examined the prolonged effects (7 days) of ilp on thyroid hormone metabolism with respect to induction and recovery of the euthyroid sick syndrome in six cancer patients. after ilp, when the limb is reconnected to the systemic circ ... | 1999 | 10094108 |
| rna-binding properties of in vitro expressed histidine-tagged rb69 rega translational repressor protein. | to facilitate rna-binding studies of the phage rb69 rega translational repressor protein, rega was configured to add six histidines to the carboxyl end of the protein. in vitro transcription-translation from the t7 promoter on plasmid psa1 yielded a rega69-his6 protein that binds nickel-sepharose and elutes with 0.5 m imidazole. the system was further modified to avoid cloning and the toxic effects of rega on escherichia coli by the polymerase chain reaction (pcr), producing linear templates wit ... | 1999 | 10094772 |
| the impact of propylthiouracil therapy on lipid peroxidation and antioxidant status parameters in hyperthyroid patients. | this study was performed on 17 hyperthyroid patients and 15 healthy controls. the patients were under propylthiouracil (ptu) therapy at a dosage of 3 x 100 mg/day for one month. blood samples, taken at the beginning and on the 30th day of therapy, were analyzed for hormonal parameters (t3, t4, tsh), lipid peroxidation endproduct [thiobarbituric acid reactive substances (tbars)] and antioxidant status parameters: glutathione (gsh), glutathione peroxidase (gsh-px) and cuzn superoxide dismutase (cu ... | 1999 | 10096735 |
| type 1 deiodinase is stimulated by iodothyronines and involved in thyroid hormone metabolism in human somatomammotroph gx cells. | local 5'-deiodination of l-thyroxine (t4) to the active thyroid hormone, 3,3',5-tri-iodothyronine (t3) via two deiodinase isoenzymes (d1 and d2) has an important role for various t3-dependent functions in the anterior pituitary. however, no evidence has been presented yet for thyroid hormone inactivation via the 5-deiodinase (d3) in anterior pituitary models. | 1999 | 10097258 |
| [retrospective study of complications of surgery for laryngeal cancer]. | surgery, alone or in combination with other therapeutic measures, is one of the main approaches to curing laryngeal cancer. the risk of complications is implicit in any surgical procedure. we describe our experience with general and local complications in surgery for laryngeal cancer and examine their relation to tumor extension and surgical technique. a review was made of a series of 431 patients who underwent surgery for laryngeal cancer over a 10-year period (1982-1991). twenty-two patients ( ... | 1999 | 10091350 |
| low p27 expression correlates with poor prognosis for patients with oral tongue squamous cell carcinoma. | p27, a cyclin-dependent kinase inhibitor, regulates progression from g1 to s phase. there have been a few clinical reports of low p27 expression associated with poor survival among patients with cancer; however, there have been no reports of such an association in cases of head and neck cancer. the authors investigated whether p27 expression in patients with oral tongue squamous cell carcinoma was associated with their prognosis. | 1999 | 10091782 |
| factors influencing contralateral lymph node metastasis from oral carcinoma. | an ipsilateral neck dissection is mandatory during initial treatment stages ii-iv oral carcinomas. however, no consensus exists whether or not to perform an elective contralateral neck dissection. | 1999 | 10091977 |
| in utero methylmercury exposure differentially affects the activities of selenoenzymes in the fetal mouse brain. | pregnant icr mice were subcutaneously injected with 0,5, or 3x3 mg hg/kg of methylmercury (mehg) on days 12,13, and 14(g12-14) of gestation and were sacrificed on g17. activity of selenoenzymes, including glutathione peroxidase (gpx) and 5'- or 5-iodothyronine deiodinases (5'-di, 5-di), was determined in fetal brain and placenta. mehg did not affect the concentration of se in these tissues, while it significantly inhibited the activity of gpx in the fetal brain and placenta, but not in the mater ... | 1999 | 10092441 |
| detection of anticodon nuclease residues involved in trnalys cleavage specificity. | the trnalys-specific anticodon nuclease exists in latent form in escherichia coli strains containing the optional prr locus. the latency is a result of a masking interaction between the anticodon nuclease core-polypeptide prrc and the type ic dna restriction-modification enzyme ecoprri. activation of the latent enzyme by phage t4-infection elicits cleavage of trnalys 5' to the wobble base, yielding 5'-oh and 2', 3'-cyclic phosphate termini. the n-proximal half of prrc has been implicated with (a ... | 1999 | 10092455 |
| the catalytic mechanism of a pyrimidine dimer-specific glycosylase (pdg)/abasic lyase, chlorella virus-pdg. | the repair of uv light-induced cyclobutane pyrimidine dimers can proceed via the base excision repair pathway, in which the initial step is catalyzed by dna glycosylase/abasic (ap) lyases. the prototypical enzyme studied for this pathway is endonuclease v from the bacteriophage t4 (t4 bacteriophage pyrimidine dimer glycosylase (t4-pdg)). the first homologue for t4-pdg has been found in a strain of chlorella virus (strain paramecium bursaria chlorella virus-1), which contains a gene that predicts ... | 1999 | 10092668 |
| n-3 enrichment of chicken meat using fish oil: alternative substitution with rapeseed and linseed oils. | two sequential experiments were conducted to assess the effect of replacing a fish oil diet with vegetable oil diets on broiler chicken performance and fatty acid (fa) composition and sensory traits of broiler meat. a diet enriched with 8.2% fish oil (fo) was fed to the birds throughout the 5-wk growth period (t1), the same basal diet being supplemented with 8.2% linseed oil (lo, experiment 1) or rapeseed oil (ro, experiment 2) in three different periods: the last week before slaughtering at 35 ... | 1999 | 10090262 |
| autosomally transmitted low concentration of thyroxine-binding globulin. | the gene coding for thyroxine-binding globulin (tbg) is located on the long arm of the x chromosome; thus anomalies of tbg are transmitted in an x-linked fashion. we report a family with decreased concentration of circulating tbg and an unusual pattern of inheritance. in this family, a number of members showed decreased levels of serum tbg. the unusual finding was that the low tbg was transmitted from the male proband to his son. thyroxine (t4) binding affinity and heat lability of serum tbg of ... | 1999 | 10090316 |
| a novel resistance to thyroid hormone associated with a new mutation (t329n) in the thyroid hormone receptor beta gene. | resistance to thyroid hormone (rth) is a syndrome of elevated serum thyroxine, inappropriately "normal" serum thyrotropin (tsh) and reduced thyroid hormone responsiveness associated with point mutations in the thyroid hormone receptor-beta (trbeta) gene. we describe a novel point mutation resulting in a cytosine for adenine substitution at nucleotide 1271 (exon 9) that results in the substitution of threonine for asparagine (t329n). this mutation was identified in a 30-year-old woman who was inv ... | 1999 | 10090317 |
| potential mechanisms of thyroid disruption in humans: interaction of organochlorine compounds with thyroid receptor, transthyretin, and thyroid-binding globulin. | organochlorine compounds, particularly polychlorinated biphenyls (pcbs), alter serum thyroid hormone levels in humans. hydroxylated organochlorines have relatively high affinities for the serum transport protein transthyretin, but the ability of these compounds to interact with the human thyroid receptor is unknown. using a baculovirus expression system in insect cells (sf9 cells), we produced recombinant human thyroid receptor ss (htrss). in competitive binding experiments, the recombinant rece ... | 1999 | 10090705 |
| motion of spin-labeled side chains in t4 lysozyme: effect of side chain structure. | previous studies have shown that the mobility of nitroxide side chains in a protein, inferred from the electron paramagnetic resonance (epr) spectra, can be used to classify particular sites as helix surface sites, tertiary contact sites, buried sites, or loop sites. in addition, the sequence dependence of mobility can identify regular secondary structure. however, in the most widely used side chain, an apparent interaction of the nitroxide ring with the protein at some helix surface sites gives ... | 1999 | 10074347 |
| x-ray structure of t4 endonuclease vii: a dna junction resolvase with a novel fold and unusual domain-swapped dimer architecture. | phage t4 endonuclease vii (endo vii), the first enzyme shown to resolve holliday junctions, recognizes a broad spectrum of dna substrates ranging from branched dnas to single base mismatches. we have determined the crystal structures of the ca2+-bound wild-type and the inactive n62d mutant enzymes at 2.4 and 2.1 a, respectively. the endo vii monomers form an elongated, highly intertwined molecular dimer exhibiting extreme domain swapping. the major dimerization elements are two pairs of antipara ... | 1999 | 10075917 |
| a prospective pilot study of extended (d3) and superextended para-aortic lymphadenectomy (d4) in patients with t3 or t4 gastric cancer managed by total gastrectomy. | japanese surgeons have been actively performing extended lymphadenectomy (d2, removal of perigastric nodes and nodes along the left gastric, common hepatic, celiac and splenic arteries; or d3, d2 plus removal of nodes in the hepatoduodenal ligament, in the retropancreatic space and along the vessels of the transverse mesocolon). in recent years interest has expanded to superextended lymphadenectomy (d4) of nodes around abdominal aorta (para-aortic lymph nodes from aortic hiatus to aortic bifurca ... | 1999 | 10076618 |
| free fibula osteoseptocutaneous-pedicled pectoralis major myocutaneous flap combination in reconstruction of extensive composite mandibular defects. | lateral composite mandibular defects resulting from excision of advanced oral carcinoma often require mandible, intra-oral lining, external face, and soft-tissue bulk reconstruction. ignorance of importance soft-tissue deficit in those patients may cause significant morbidity and functional loss. such defects, therefore, can be reconstructed best with a double free flap technique. however, this procedure may not be feasible for every patient or surgeon. an alternative procedure is a free fibula ... | 1999 | 10077072 |
| blunted nocturnal tsh surge does not indicate central hypothyroidism in patients after pituitary surgery. | the thyrotropin-releasing hormone stimulation test (trh test) is commonly used as part of the endocrine evaluation after pituitary surgery. however, some patients with a normal thyrotropin (tsh) response to trh after pituitary surgery develop central hypothyroidism during follow-up. on the other hand, hypothyroidism does not necessarily ensue in patients with a blunted tsh response. as tsh is secreted in a pulsatile fashion with maximum secretion in the early morning, we investigated whether mea ... | 1999 | 10077363 |
| structural and functional analysis of surface domains unique to bacteriophage t4 thymidylate synthase. | the bacteriophage t4 genome encodes most of its own enzymes for dntp synthesis, which form a complex in infected escherichia coli. the t4 thymidylate synthase (ts) and the t4 deoxycytidylate deaminase (cd) catalyze sequential reactions and are physically linked within this complex [mcgaughey, k. m., wheeler, l. j., moore, j. t., maley, g. f. , maley, f., and mathews, c. k. (1996) j. biol. chem. 271, 23037-23042]. from the crystal structure of t4ts [finer-moore, j. s., maley, g. f., maley, f., mo ... | 1999 | 10026292 |
| long-term calorie restriction reduces energy expenditure in aging monkeys. | calorie restriction to produce stable long-term adult body weight for approximately 10 years prevents obesity and diabetes in middle-aged rhesus monkeys. to determine whether this dietary regimen also alters energy metabolism, the doubly labeled water method was used to measure total daily energy expenditure. six adult male rhesus monkeys, which had been calorie-restricted for more than 10 years, were compared to 8 control adult monkeys, which had been fed ad libitum for their entire lives. the ... | 1999 | 10026648 |
| familial prostate cancer: outcome following radiation therapy with or without adjuvant androgen ablation. | to compare the outcome of familial versus sporadic prostate carcinoma after definitive external radiation. | 1999 | 10030265 |
| modified staging system for extremity soft tissue sarcomas. | the establishment of a universally acceptable staging system for soft tissue sarcomas has been hampered by the low incidence, various grading systems, and lack of consensus regarding the value of different prognostic factors. we aimed to evaluate prognostic factors in patients with extremity soft tissue sarcomas and to test the validity of the ajcc/uicc staging system. | 1999 | 10030416 |
| phenobarbital, beta-naphthoflavone, clofibrate, and pregnenolone-16alpha-carbonitrile do not affect hepatic thyroid hormone udp-glucuronosyl transferase activity, and thyroid gland function in mice. | the effects of representative liver enzyme inducers such as clofibrate (clo), phenobarbital (pb), pregnenolone-16alpha-carbonitrile (pcn), and beta-naphthoflavone (nf) on hepatic microsomal thyroxin (t4)- udp-glucuronosyl transferase (ugt) and triiodothyronine (t3)- ugt activities and thyroid function were evaluated in of-1 male mice after a 14-day po administration. clo, pb, and pcn induced histological liver hypertrophy, increases in liver weights, in microsomal protein and cytochrome p450 con ... | 1999 | 10036213 |
| inhibition by lead of production and secretion of transthyretin in the choroid plexus: its relation to thyroxine transport at blood-csf barrier. | long-term, low-dose pb exposure in rats is associated with a significant decrease in transthyretin (ttr) concentrations in the csf. since csf ttr, a primary carrier of thyroxine in brain, is produced and secreted by the choroid plexus, in vitro studies were conducted to test whether pb exposure interferes with ttr production and/or secretion by the choroid plexus, leading to an impaired thyroxine transport at the blood-csf barrier. newly synthesized ttr molecules in the cultured choroidal epithe ... | 1999 | 10036215 |
| increased risk of maternal thyroid failure with pregnancy progression in an iodine deficient area with major iodine deficiency disorders. | in an effort to assess the impact of moderate iodine deficiency on maternal thyroid function during pregnancy, we measured serum thyrotropin, total and free thyroid hormones, thyroid-binding globulin (tgb) at 8, 14, 20, 29, and 36 weeks of gestation, along with urinary iodide excretion, in 10 healthy women from a moderately iodine deficient region (group a), and compared them with 6 women from an iodine sufficient region (group b). serum total thyroxine (t4) fell significantly in group a, and wa ... | 1999 | 10037071 |
| thyroid function in the preterm infant. | thyroid gland function develops and matures during fetal life, with production of serum thyroxine (t4) concentrations beginning around 12 weeks gestation and increasing to term. infants born prior to term have lower cord serum t4 concentrations that correlate with gestational age or birth weight. this is partially the result of lower thyroxine-binding globulin (tbg) concentrations. the cord serum free thyroxine (ft4) concentrations also correlate with gestational age, but they are not proportion ... | 1999 | 10037080 |
| neonatal hypothyroidism: treatment and outcome. | systematic neonatal screening for congenital hypothyroidism (ch), which was progressively implemented in industrialized countries over the past 15 to 25 years, has been extremely successful in eradicating severe mental deficiency resulting from ch. however, in the first generation of children diagnosed by screening, the concept that ch has a threshold effect on intelligence was confirmed. in spite of earlier diagnosis through screening, children with severe ch (i.e., those with a marked retardat ... | 1999 | 10037081 |
| thyroid function in very preterm newborns: possible implications. | thyroid hormones are essential for brain maturation. very preterm infants, who are at risk of neurodevelopmental disabilities also have low thyroxine (t4) and free thyroxine (ft4) values in the first weeks after birth. this transient hypothyroxinemia may in part be causal to the neurodevelopmental problems. we have carried out a randomized, double-blind, placebo-controlled trial with t4 in 200 infants less than 30 weeks gestation. t4 (or placebo) was given in fixed dose of 8 microg/kg birth weig ... | 1999 | 10037082 |
| in vitro selection of sequence contexts which enhance bypass of abasic sites and tetrahydrofuran by t4 dna polymerase holoenzyme. | the influence of sequence context on the ability of dna polymerase to bypass sites of base loss was addressed using an in vitro selection system. oligonucleotides containing either an aldehydic abasic site or tetrahydrofuran surrounded by four randomized bases on both the 5' and 3' sides were used as templates for synthesis by phage t4 dna polymerase holoenzyme proficient or deficient in the 3'-->5' proofreading exonuclease activity. successful bypass products were purified, subcloned and the se ... | 1999 | 10047481 |
| details of the nucleic acid binding site of t4 gene 32 protein revealed by proteolysis and dna tm depression methods. | the affinities and location of oligonucleotides bound to intact and truncated bacteriophage t4 gene 32 protein have been elucidated by two independent and sensitive methods. the nucleic acid binding site is located within the core domain of 32 protein, residues 22-253. oligonucleotides protect the core domain against proteolysis catalyzed by mammalian endoproteinase arg-c. of the three cleavage sites, arg111, within the internal "last" ((lys/arg)3(ser/thr)2) motif, is selectively protected. we h ... | 1999 | 10047485 |
| mouse testicular extracts process dna double-strand breaks efficiently by dna end-to-end joining. | dna double-strand break (dsb) processing was studied in mouse testicular extracts using a defined dsb created by cleaving supercoiled puc12 dna at a unique site as the substrate, and analysing the processed dna by gel electrophoresis. our results demonstrated that enzymatic activity in the extracts promoted multimerization of dna and suppressed its circularization. this was distinctly different from t4 dna ligase activity in the control and therefore the process must be more complex than simple ... | 1999 | 10047774 |
| photoprotection by topical dna repair enzymes: molecular correlates of clinical studies. | a new approach to photoprotection is to repair dna damage after uv exposure. this can be accomplished by delivery of a dna repair enzyme with specificity to uv-induced cyclobutane pyrimidine dimers into skin by means of specially engineered liposomes. treatment of dna-repair-deficient xeroderma pigmentosum patients or skin cancer patients with t4n5 liposome lotion containing such dna repair liposomes increases the removal of dna damage in the first few hours after treatment. in these studies, a ... | 1999 | 10048308 |
| gadd45, a p53-responsive stress protein, modifies dna accessibility on damaged chromatin. | this report demonstrates that gadd45, a p53-responsive stress protein, can facilitate topoisomerase relaxing and cleavage activity in the presence of core histones. a correlation between reduced expression of gadd45 and increased resistance to topoisomerase i and topoisomerase ii inhibitors in a variety of human cell lines was also found. gadd45 could potentially mediate this effect by destabilizing histone-dna interactions since it was found to interact directly with the four core histones. to ... | 1999 | 10022855 |
| transneuronal tracing of neural pathways controlling an abdominal muscle, rectus abdominis, in the ferret. | abdominal muscles participate in generating a large number of behaviors and reflex responses, including expiration, coughing, sneezing, vomiting, postural control, production of speech, straining, facilitation of venous return to the heart, and reaction to vestibular stimulation. however, the only premotor neurons that have been conclusively shown to influence abdominal motoneurons are located in nucleus retroambiguus, the expiratory region of the caudal ventral respiratory group. in the present ... | 1999 | 10023028 |
| endocrine regulation of fetal adipose tissue metabolism in the pig: interaction of porcine growth hormone and thyroxine. | this study tested the hypothesis that combined treatment of thyroxine (t4) and growth hormone (gh) could normalize cellular and metabolic aspects of adipose tissue development of hypophysectomized fetal pigs. | 1999 | 10023733 |
| effects of microbial phytase supplementation on egg production, eggshell quality, and mineral retention of laying hens fed different levels of phosphorus. | a 20-wk feeding trial (21 to 40 wk of age) was conducted to evaluate the effects of phytase supplementation on egg production, egg quality, nutrient retention, and p excretion of laying hens fed diets containing different levels of p. nine hundred and sixty isa brown hens were randomly allocated to completely randomized block arrangement of four diets: corn-soybean diet (1.4% tricalcium phosphate, tcp) without (t1, control) and with phytase (t2); 0.7% tcp (t3) or 0% tcp (t4) diet with phytase. d ... | 1999 | 10023751 |
| decreasing levels of anti-nef antibody correlate with increasing hiv type 1 viral loads and aids disease progression. | to study the association between anti-gag and anti-nef antibody reactivities and their correlations with disease progression, 174 hiv-1/aids patients were followed up for 1 year after they received triple therapy. the antibody reactivities were analyzed using a western blot test with recombinant gag and nef proteins. the results showed that decreasing levels of anti-gag or anti-nef antibody correlate with disease progression defined by hiv-1 viral loads or t4 cell counts. after receiving triple ... | 1999 | 10024051 |
| leptin in women with eating disorders. | the aim of the present study was to determine the factors controlling leptin secretion and to clarify the role of leptin in eating disorders. the subjects were 152 eating-disordered women with different fat mass, eating behavior, and endocrine abnormalities and 24 age-matched control subjects. the body fat mass, eating behavior score, and plasma leptin, luteinizing hormone (lh), follicle-stimulating hormone (fsh), triiodothyronine (t3), free thyroxine (t4), insulin, and cortisol levels were eval ... | 1999 | 10024085 |
| water enema transvaginal ultrasound for local staging of stenotic rectal carcinoma. | to increase the value of ultrasound in the staging of stenotic rectal carcinoma. | 1999 | 10024397 |
| thyroid hormone promotes the phosphorylation of stat3 and potentiates the action of epidermal growth factor in cultured cells. | we have examined the effects of l-thyroxine (t4) on the activation of signal transducer and activator of transcription 3 (stat3) and on the stat3-dependent induction of c-fos expression by epidermal growth factor (egf). t4, at a physiological concentration of 100 nm, caused tyrosine phosphorylation and nuclear translocation (i.e. activation) of stat3 in hela cells in as little as 10-20 min. activation by t4 of stat3 was maximal at 30 min (15+/-4-fold enhancement; mean+/-s.e.m.) in 18 experiments ... | 1999 | 10024519 |
| [reconstruction of great vessel for patients with advanced lung cancer or malignant mediastinal tumor]. | fifteen lung cancer (lc) and 11 malignant mediastinal tumor (mmt) patients who received surgical treatment in association with a reconstruction of great vessel from 1981 to 1997 were retrospectively reviewed. the aorta was resected in 7 (ao group) of 15 lc patients and the superior vena cava in the remaining 8 patients (svc group). pathological examination revealed actual tumor invasion in one (14%) of ao group and 6 (75%) of svc group. in 7 p-t4 patients, 6 (85.7%) were p-n2 or n3 diseases. fiv ... | 1999 | 10024797 |
| serum cytokines in thyrotoxicosis. | overproduction of thyroid hormones promotes bone resorption in vivo and in vitro, and we have evaluated whether mediators of such effects could include the osteotropic cytokines. previous studies have demonstrated raised serum interleukin (il)-6 in thyrotoxic patients, but differentiating the contribution of the elevated thyroid hormones from that of the autoimmune inflammation present in graves' disease (gd) has been difficult. we undertook a longitudinal study of 34 patients (19-45 yr old) wit ... | 1999 | 10022397 |
| comparison of radioiodine with radioiodine plus lithium in the treatment of graves' hyperthyroidism. | effectiveness of radioiodine for graves' hyperthyroidism depends also on its intrathyroidal persistence. the latter is enhanced by lithium by blocking iodine release from the thyroid. one hundred ten patients with graves' hyperthyroidism were randomly assigned to treatment with radioiodine or radioiodine plus lithium, stratified according to goiter size (< or =40 or >40 ml) and evaluated for changes in thyroid function and goiter size, at monthly intervals, for 12 months. cure of hyperthyroidism ... | 1999 | 10022407 |
| is routine thyroxine treatment to hinder postoperative recurrence of nontoxic goiter justified? | previous reports regarding the efficacy of levo-t4 (l-t4) in preventing postoperative recurrence of nontoxic goiter have been controversial. this study was designed to evaluate the influence of long-term l-t4 treatment on thyroid volume after thyroidectomy for nontoxic goiter. we studied 202 consecutive patients operated on for benign nontoxic goiter and followed them for a minimum of 12 months (median, 10 yr; range, 1-14 yr). three months after thyroidectomy, patients were randomized to l-t4 tr ... | 1999 | 10022449 |
| involvement of spinal nk1 and opioids receptors in modulating the inhibitory effect of capsaicin on micturition reflex in the acute spinalized guinea pig. | the aim of this work was to study the role of capsaicin-sensitive afferent fibers in modulating the micturition reflex at spinal level in urethane-anesthetized guinea pigs after spinal cord transection at level t3-t4. | 1999 | 10022740 |
| uterine sarcomas: the curie institut experience. prognosis factors and adjuvant treatments. | with a retrospective study at a single institution, we propose to analyze the prognosis factors and adjuvant treatment for uterine sarcomas. | 1999 | 10021306 |
| the 1997 international staging system for non-small cell lung cancer: have all the issues been addressed? | the international staging system for lung cancer has been revised recently. important changes have been made to allow better correlation of prognoses and direction of management. the classification of synchronous pulmonary nodules in the same lobe as the primary tumor as t4 stage iiib may imply a poorer outcome than is warranted, while the designation of a similar stage for malignant pleural effusion may not be reflective of the very poor prognosis associated with this extent of disease. | 1999 | 9925091 |
| [investigation and treatment of solitary thyroid gland nodules by danish endocrinologists. a questionnaire study]. | in order to evaluate the attitude among danish specialists in endocrinology towards the management of the solitary thyroid nodule a questionnaire study, based on a case report of a 42-year old woman with a solitary 2 x 3 cm thyroid nodule and absence of clinical suspicion of malignancy, was performed. routine in vitro tests included serum tsh in all and ft4 and total t4 in more than half of the respondents. 99mtc scintigraphy and ultrasonography (us) were included by nearly 100% of the clinician ... | 1999 | 10083823 |
| [prolonged suppressive l-thyroxine therapy. longitudinal study of the effect of lt4 on bone mineral density and bone metabolism markers in 71 patients]. | a prospective longitudinal study was conducted to investigate the influence of prolonged suppressive l-thyroxin therapy on bone density and biochemical markers of bone remodeling. | 1999 | 10083873 |
| discrimination of dna duplexes with matched and mismatched tandem repeats by t4 endonuclease vii. | a simple in situ method for scoring short tandem dna repeat length has been developed using t4 endonuclease vii. this method measures tandem repeated simple sequences embedded in unique sequences. single-stranded loops are formed on duplexes containing mismatched (different) numbers of tandem repeats. no single stranded loops are formed on structures containing matched (identical) numbers of tandem repeats. the matched and mismatched loop structures were distinguished and differentially labeled ... | 1999 | 10084111 |
| anemia and carnitine supplementation in hemodialyzed patients. | carnitine supplementation in hemodialyzed patients was studied in a double-blinded, randomized, controlled trial in order to elucidate the effect of intravenous carnitine on renal anemia in patients treated with recombinant human erythropoietin (rhuepo). twenty stable hemodialysis (hd) patients received intravenous l-carnitine after each dialysis session in a dosage of 5 (n = 15) and 25 (n = 5) mg/kg, respectively, together with intravenous iron saccharate (20 mg/hd session) for four months and ... | 1999 | 10084293 |
| functional hypothalamic amenorrhea: hypoleptinemia and disordered eating. | because the exact etiology of functional, or idiopathic, hypothalamic amenorrhea (fha) is still unknown, fha remains a diagnosis of exclusion. the disorder may be stress induced. however, mounting evidence points to a metabolic/nutritional insult that may be the primary causal factor. we explored the thyroid, hormonal, dietary, behavior, and leptin changes that occur in fha, as they provide a clue to the etiology of this disorder. fourteen cycling control and amenorrheic nonathletic subjects wer ... | 1999 | 10084564 |
| evaluation of the adequacy of levothyroxine replacement therapy in patients with central hypothyroidism. | as there are few data on the evaluation of the adequacy of levothyroxine (l-t4) therapy in patients with central hypothyroidism (ch), a prospective study was performed to assess the accuracy of various parameters in the follow-up of 37 ch patients. total and free thyroid hormones, tsh, and a series of clinical and biochemical indexes of peripheral thyroid hormone action have been evaluated off and on l-t4 therapy. samples were taken before the daily administration of l-t4. in all patients off th ... | 1999 | 10084572 |
| standard free energy for the hydrolysis of adenylylated t4 dna ligase and the apparent pka of lysine 159. | equilibrium constants for the adenylylation of t4 dna ligase have been measured at 10 ph values. the values, when plotted against ph, fit a titration curve corresponding to a pka of 8.4 +/- 0.1. the simplest interpretation is that the apparent pka is that of the 6-amino group of the amp-accepting residue lys159. based on the ph dependence of the equilibrium constants, the value at ph 7.0 is 0. 0213 at 25 degrees c, corresponding to deltag'o = +2.3 kcal mol-1. from this value and the standard fre ... | 1999 | 10085093 |
| recent advances in understanding thyroid hormone receptor coregulators. | thyroid hormones (l-triiodothyronine, t3; l-tetraiodothyronine, t4) regulate normal cellular growth and development, and general metabolism as well. their various actions are mediated by the thyroid hormone receptor, a ligand-dependent transcriptional factor belonging to the nuclear hormone receptor superfamily. the recent discovery of coregulators (coactivators, corepressors, and cointegrators) has greatly enhanced our understanding of thyroid hormone receptor functions. hence we review and dis ... | 1999 | 10087437 |
| evaluation of serum free thyroxine and thyrotropin concentrations in the diagnosis of canine hypothyroidism. | canine thyroid-stimulating hormone (ctsh), total thyroxine (t4) and free t4 by equilibrium dialysis (ft4d) were measured in serum samples from 107 dogs with clinical signs suggestive of hypothyroidism in which the diagnosis was either confirmed (n = 30) or excluded (n = 77) by exogenous tsh response testing. median serum total t4 and ft4d concentrations were significantly lower and ctsh significantly higher (p < 0.001) in hypothyroid compared with euthyroid dogs. differential positive rate analy ... | 1999 | 10088086 |
| influence of local tumour stage and grade on reliability of serum prostate-specific antigen in predicting skeletal metastases in patients with adenocarcinoma of the prostate. | to determine whether serum prostate-specific antigen (psa) can be reliably used to predict the absence or presence of skeletal metastases on the bone scan in patients with adenocarcinoma of the prostate. | 1999 | 10072624 |
| thyroid functions in lithium-treated psychiatric patients: a cross-sectional study. | in the present cross-sectional study, thyroid functions (viz. thyroid radioiodine uptake [raiu] and serum t3, t4, and thyroid-stimulating hormone [tsh]) were evaluated in 24 healthy controls and 132 outdoor affective disorder patients. eleven of these patients were to receive lithium (li) and the remaining 121 patients were at different stages of li treatment ranging from 0.7 to 240 mo. raiu was found to increase significantly throughout the li therapy and was associated with the corresponding r ... | 1999 | 10073421 |
| the effects of dexmedetomidine on neuromuscular blockade in human volunteers. | the neuromuscular effects of dexmedetomidine in humans are unknown. we evaluated the effect of dexmedetomidine on neuromuscular block and hemodynamics during propofol/alfentanil anesthesia. during propofol/alfentanil anesthesia, the rocuronium infusion rate was adjusted in 10 volunteers to maintain a stable first response (t1) in the train-of-four sequence at 50% +/- 3% of the pre-rocuronium value. dexmedetomidine was then administered by computer-controlled infusion, targeting a plasma dexmedet ... | 1999 | 10072019 |
| crystal structure of deoxycytidylate hydroxymethylase from bacteriophage t4, a component of the deoxyribonucleoside triphosphate-synthesizing complex. | bacteriophage t4 deoxycytidylate hydroxymethylase (ec 2.1.2.8), a homodimer of 246-residue subunits, catalyzes hydroxymethylation of the cytosine base in deoxycytidylate (dcmp) to produce 5-hydroxymethyl-dcmp. it forms part of a phage dna protection system and appears to function in vivo as a component of a multienzyme complex called deoxyribonucleoside triphosphate (dntp) synthetase. we have determined its crystal structure in the presence of the substrate dcmp at 1.6 a resolution. the structur ... | 1999 | 10064578 |
| video-assisted thoracoscopic esophagectomy for esophageal cancer. | the ivor-lewis procedure is a radical, invasive, and effective procedure for the resection of most esophageal cancers. to minimize invasiveness, we performed thoracoscopic and video-assisted esophagectomy and mediastinal dissection for esophageal cancer. | 1999 | 10064749 |
| dna polymerase mutagenic bypass and proofreading of endogenous dna lesions. | dna polymerases differentiate between correct and incorrect substrates during synthesis on undamaged dna templates through the biochemical steps of base incorporation, primer-template extension and proofreading excision. recent research examining dna polymerase processing of abasic, alkylation and oxidative lesions is reviewed in light of these discrimination mechanisms. inhibition of dna synthesis results from correct polymerase discrimination against utilization of geometrically incorrect temp ... | 1999 | 10064863 |
| 'saccharomyces cerevisiae msh2/6 complex interacts with holliday junctions and facilitates their cleavage by phage resolution enzymes. | genetic and biochemical studies have indicated that mismatch repair proteins can interact with recombination intermediates. in this study, gel shift assays and electron microscopic analysis were used to show that the saccharomyces cerevisiae msh2/6 complex binds to holliday junctions and has an affinity and specificity for them that is at least as high as it has as for mispaired bases. under equilibrium binding conditions, the msh2/6 complex had a kd of binding to holliday junctions of 0.5 nm. t ... | 1999 | 10066781 |
| thyroid hormones act primarily within the brain to promote the seasonal inhibition of luteinizing hormone secretion in the ewe. | in the ewe, thyroid hormones are required for the seasonal suppression of gnrh and lh secretion, thereby maintaining an annual rhythm in reproductive activity. the primary site of action of thyroid hormones is unknown; in particular, there is no evidence to distinguish a central from a peripheral action. in this study, we test the hypothesis that thyroid hormones can act directly within the brain to promote gnrh/lh seasonal inhibition. ovariectomized estradiol-treated ewes were thyroidectomized ... | 1999 | 10067833 |
| regulation and transfer of a murine model of thyrotropin receptor antibody mediated graves' disease. | in order to replicate a recently described murine model of graves' disease, we immunized akr/n (h-2k) mice i.p., every 2 weeks, with either a clone of fibroblasts expressing both the human tsh receptor (htshr) and murine major histocompatibility complex (mhc) class ii molecules or with fibroblasts expressing the mhc class ii molecules alone. mice were bled, and their thyroid hormone levels measured, at 6, 12, and up to 18 weeks after the first immunization. between 11-12 weeks after immunization ... | 1999 | 10067867 |
| characterization of thyroid hormone 5'-monodeiodinase activity in the turtle (trachemys scripta). | thyroid hormone metabolism by 5'-monodeiodinase enzymes (5'md) was characterized in peripheral tissues of the turtle, trachemys scripta, and compared with activity measured in the rat. based on differences in ph dependence, sensitivity to inhibitors, substrate affinity, and cofactor requirements, at least two types of enzyme activities have been identified in the turtle. a 5'md activity was measured in liver and kidney microsomal fractions that exhibits inhibition by 2n-propyl-6-thiouracil (ptu) ... | 1999 | 10068496 |
| seasonal changes in mrna levels of gonadotropin and thyrotropin subunits in the goldfish, carassius auratus. | seasonal changes in the mrna levels of glycoprotein alpha, gonadotropin (gth) ibeta and iibeta, and thyrotropin (thyroid-stimulating hormone (tsh)) beta subunits in the pituitary of goldfish were quantified by northern blot analysis and laser densitometry. reproductive development and thyroid activity were monitored by measuring gonadosomatic index, plasma gth ii, testosterone (t), estradiol, 11-ketotestosterone, and thyroid hormones (t4 and t3). plasma gth ii and steroids showed characteristic ... | 1999 | 10068504 |
| total intravenous anesthesia with remifentanil and propofol for implantation of cardioverter-defibrillators in patients with severely reduced left ventricular function. | to determine the cardiocirculatory effects of total intravenous anesthesia (tiva) using remifentanil and propofol in high-risk cardiac surgical patients. | 1999 | 10069277 |
| prognosis in cutaneous t-cell lymphoma by skin stage: long-term survival in 489 patients. | although a number of studies have documented the long-term survival of patients with cutaneous t-cell lymphoma (ctcl), none have provided data as to the relative survival of all 4 skin stages. | 1999 | 10071312 |
| glycosylated ectodomain of the human thyrotropin receptor induces antibodies capable of reacting with multiple blocking antibody epitopes. | recently, we showed that the glycosylated ectodomain of the human thyrotropin receptor (het-gp) reacts with autoantibodies from autoimmune thyroid disease (aitd) patients' sera. to better understand the effects of glycosylation of thyrotropin receptor (tshr) in antibody induction, we immunized rabbits with het-gp protein. the rabbits developed relatively high titers of antibodies with highly potent tsh binding inhibitory immunoglobulin (tbii) and thyroid stimulatory blocking antibody (tsbab) act ... | 1999 | 10052682 |
| model for bacteriophage t4 development in escherichia coli. | mathematical relations for the number of mature t4 bacteriophages, both inside and after lysis of an escherichia coli cell, as a function of time after infection by a single phage were obtained, with the following five parameters: delay time until the first t4 is completed inside the bacterium (eclipse period, nu) and its standard deviation (sigma), the rate at which the number of ripe t4 increases inside the bacterium during the rise period (alpha), and the time when the bacterium bursts (mu) a ... | 1999 | 10049403 |
| video-assisted thoracic diskectomy and anterior release: a biomechanical analysis of an endoscopic technique. | this study evaluates the residual biomechanical stability of the spine following multilevel anterior diskectomies and anterior longitudinal ligament release using video-assisted thoracoscopic surgery (vats). eighteen domestic pigs were randomly divided into three groups of six pigs. group 1 underwent thoracic anterior release from t4-t9 using a left-sided vats approach, group 2 underwent thoracic anterior release from t4-t9 via a traditional left thoracotomy (open), and group 3 did not undergo s ... | 1999 | 10535554 |
| increased glucose synthesis in renal tubule fragments from hyperthyroid rats. | rates of glucose synthesis from several substrates were examined in renal tubule fragments from hyperthyroid rats. a hyperthyroid state was induced by daily intraperitoneal injections of thyroxine (t4) (100 microg/100 g body weight) for 14 days. at the end of the experimental period, plasma triiodothyronine and t4 levels were six and eight times higher, respectively, than initial values. hyperthyroid rats gained less weight and had lower blood glucose despite an increased food intake. in both co ... | 1999 | 10535706 |
| dna packing in stable lipid complexes designed for gene transfer imitates dna compaction in bacteriophage. | the structure of complexes made from dna and suitable lipids (lipoplex, lx) was examined by cryo-electron microscopy (cryoem). we observed a distinct concentric ring-like pattern with striated shells when using plasmid dna. these spherical multilamellar particles have a mean diameter of 254 nm with repetitive spacing of 7.5 nm with striation of 5.3 nm width. small angle x-ray scattering revealed repetitive ordering of 6.9 nm, suggesting a lamellar structure containing at least 12 layers. this co ... | 1999 | 10535915 |
| opening of a monomer-monomer interface of the trimeric bacteriophage t4-coded gp45 sliding clamp is required for clamp loading onto dna. | the replication system of bacteriophage t4 uses a trimeric ring-shaped processivity clamp (gp45) to tether the replication polymerase (gp43) to the template-primer dna. this ring is placed onto the dna by an atpase-driven clamp-loading complex (gp44/62) where it then transfers, in closed form, to the polymerase. it generally has been assumed that one of the functions of the loading machinery is to open the clamp to place it around the dna. however, the mechanism by which this occurs has not been ... | 1999 | 10535942 |
| comparison of a new piezoelectric train-of-four neuromuscular monitor, the paragraph, and the relaxometer mechanomyograph. | the paragraph is a new device for monitoring neuromuscular function using a piezoelectric motion sensor. in 20 patients, monitoring of neuromuscular block produced by cisatracurium 0.1 mg kg-1 was compared using the paragraph and a relaxometer 2 mechanomyograph. the paragraph was quick to set up, and easy to operate and interpret. there were no significant differences in the time to 100% depression of t1/t0, time to 25% recovery of t1/t0 or time to recovery of t1/t0 from 25% to 75%, measured by ... | 1999 | 10536564 |
| thyroid hormone regulates the extracellular organization of laminin on astrocytes. | astrocytes produce laminin, a key extracellular matrix guidance molecule in the developing brain. laminin is bound to transmembrane receptors on the surface of astrocytes known as integrins, which are, in turn, bound to the microfilament meshwork inside the astrocyte. previous studies have shown that t4 regulates the pattern of integrin distribution in astrocytes by modulating the organization of the microfilaments. in this study, the effect of thyroid hormone on the secretion and topology of la ... | 1999 | 10537126 |
| type 3 iodothyronine deiodinase is selectively expressed in areas related to sexual differentiation in the newborn rat brain. | thyroid hormone (t4 and t3) concentrations in target tissues are greatly influenced by the activity of iodothyronine deiodinases. type 1 and 2 deiodinases generate t3 from t4, while deiodinase type 3 (d3) transforms t4 and t3 to inactive metabolites. coordination of the expression and activity of these enzymes is postulated to play an important role in physiology and development, making it possible that individual cells and tissues regulate the concentrations of the active hormone according to s ... | 1999 | 10537178 |
| long-term results of radiotherapy alone for carcinoma of the vulva. | from 1953 to 1978, definitive radiotherapy for carcinoma of the vulva without surgery was used as a standard therapy at a university hospital in germany. we retrospectively reviewed the records of 170 patients treated mainly with electron-beam radiation to the vulva and co-60 to the inguinal lymph nodes. total doses to the vulva ranged from 6 to 90 gy. a 30-year retrospective follow-up was accomplished. tumor and node classification, tumor location, and parity were significant independent factor ... | 1999 | 10539381 |
| relationship between stimulating current and accelographic train-of-four response at the great toe. | we investigated the accelographic train-of-four response evaluated at the great toe at varying stimulating currents. fifteen adult patients undergoing elective general anaesthesia were studied. the mean current at which a supramaximal t1 value could be elicited was > 49 (9) ma [mean (sd)]. ratios of accelographic t1 values at 50, 40, 30, 20 and 10 ma to accelographic t1 value at 60 ma were 0.90 (0. 18), 0.58 (0.37), 0.38 (0.37), 0.19 (0.26) and 0.00 (0.00), respectively [mean (sd), p < 0.05 for ... | 1999 | 10540101 |
| the energetics of t4 lysozyme reveal a hierarchy of conformations. | we have used native state exchange to examine the energy landscape of the well-characterized protein t4 lysozyme. although the protein exhibits two-state behavior by traditional probes, the energy landscape determined here is much more complex. the average stability of the c-terminal subdomain is significantly higher than that for the n-terminus suggesting at least two regions of unfolding. at a more detailed level, there appears to be a broad continuum of stabilities throughout each region. the ... | 1999 | 10542101 |
| identification of the rna binding domain of t4 rega protein by structure-based mutagenesis. | the t4 translational repressor rega protein folds into two structural domains, as revealed by the crystal structure (kang, c.-h. , chan, r., berger, i., lockshin, c., green, l., gold, l., and rich, a. (1995) science 268, 1170-1173). domain i of the rega protein contains a four-stranded beta-sheet and two alpha-helices. domain ii contains a four-stranded beta-sheet and an unusual 3/10 helix. since beta-sheet residues play a role in a number of protein-rna interactions, one or both of the beta-she ... | 1999 | 10542265 |
| incidence of sideropenia and effects of iron repletion treatment in women with subclinical hypothyroidism. | sideropenia affects ca. 20% of the world population, and iron dependent anemia is the most frequent type of anemia worldwide. the aim of the study was to investigate the incidence of sideropenia and dependent anemia in patients with subtle changes of the thyroid function, such as subclinical hypothyroidism (sh). 57 women with sh and 61 euthyroid controls (cg) were studied. serum concentrations of t4, t3, tsh, anti-tpo, anti-tg, ferrum (fe), ferritin (frt) total iron binding capacity (tibc) and b ... | 1999 | 10543412 |
| preoperative chemoradiotherapy and surgery for selected non-small cell lung cancer iiib subgroups: long-term results. | preoperative chemoradiotherapy is feasible for selected patients with non-small cell lung cancer stage iiib. the aim of this investigation was to analyze long-term results after this multimodality approach and to identify subgroups with improved long-term prognosis. | 1999 | 10543470 |
| video assistance reduces complication rate of thoracoscopic sympathicotomy for hyperhidrosis. | thoracoscopic sympathicotomy has proved successful in the treatment of palmar hyperhidrosis. however, up to 8% of patients experience horner's syndrome, and about 50% show compensatory sweating. this study evaluates the role of video assistance in thoracoscopic sympathicotomy for primary hyperhidrosis of the upper limb. | 1999 | 10543476 |
| specific inhibition of the eubacterial dna ligase by arylamino compounds. | all known dna ligases catalyze the formation of a phosphodiester linkage between adjacent termini in double-stranded dna via very similar mechanisms. the ligase family can, however, be divided into two classes: eubacterial ligases, which require nad(+) as a cofactor, and other ligases, from viruses, archaea, and eukaryotes, which use atp. drugs that discriminate between dna ligases from different sources may have antieubacterial activity. we now report that a group of arylamino compounds, includ ... | 1999 | 10543760 |
| polymerization of bacteriophage t4 tail sheath protein mutants truncated at the c-termini. | gene 18 of bacteriophage t4 encodes the contractile protein of the tail sheath. previous work has shown that the full-length recombinant gene product (gp) 18 of 658 amino acid residues assembles in escherichia coli cells into a long polysheath structure. however, the gp18 mutants truncated at the n-termini form insoluble aggregates similar to inclusion bodies. in this study, six plasmid vectors expressing the recombinant gp18 proteins truncated at the c-termini have been constructed. the cdelta5 ... | 1999 | 10544047 |
| role of the c-terminal rdel motif of the myxoma virus m-t4 protein in terms of apoptosis regulation and viral pathogenesis. | the purpose of this study was to investigate the significance of the c-terminal rdel motif of the myxoma virus m-t4 protein in terms of apoptosis regulation and role in viral virulence. to accomplish this, a recombinant myxoma virus was created in which the c-terminal rdel motif of m-t4 was deleted and a selectable marker (ecogpt) was inserted immediately downstream. we hypothesized that removal of the rdel motif from m-t4 would alter the subcellular localization of the protein and provide insig ... | 1999 | 10544103 |
| atp/adp switches the higher-order structure of dna in the presence of spermidine. | in the living cellular environment, dnas exist in a compact state in the presence of a polyamine, such as spermidine. we found that the hydrolysis of atp into adp induces the folding of elongated dnas, by the single-chain observation of individual t4 dna molecules. this result is discussed in relation to the possible role of atp as a regulatory factor in genetic activity, in addition to its well-established role as an energy source. | 1999 | 10544259 |
| methionine and alanine substitutions show that the formation of wild-type-like structure in the carboxy-terminal domain of t4 lysozyme is a rate-limiting step in folding. | in an attempt to identify a systematic relation between the structure of a protein and its folding kinetics, the rate of folding was determined for 20 mutants of t4 lysozyme in which a bulky, buried, nonpolar wild-type residue (leu, ile, phe, val, or met) was substituted with alanine. methionine, which approximated the size of the original side chain but which is of different shape and flexibility, was also substituted at most of the same sites. mutations that substantially destabilize the prote ... | 1999 | 10545167 |
| identification of in vivo induced genes in actinobacillus pleuropneumoniae. | we have developed an in vivo expression technology (ivet) system to identify actinobacillus pleuropneumoniae gene promoters that are specifically induced in vivo during infection. this system is based upon an avirulent riboflavin-requiring a. pleuropneumoniae mutant and a promoter-trap vector (ptf86) that contains, in sequence, the t4 terminator, a unique bam hi site, a promoterless copy of the v. harveyi luxab genes, and a promoterless copy of the b. subtilis ribbah genes in the e. coli - a. pl ... | 1999 | 10545257 |
| the structure of bacteriophage t4 gene product 9: the trigger for tail contraction. | the t4 bacteriophage consists of a head, filled with double-stranded dna, and a complex contractile tail required for the ejection of the viral genome into the escherichia coli host. the tail has a baseplate to whïch are attached six long and six short tail fibers. these fibers are the sensing devices for recognizing the host. when activated by attachment to cell receptors, the fibers cause a conformational transition in the baseplate and subsequently in the tail sheath, which initiates dna ejec ... | 1999 | 10545330 |