Publications

thyroxine replacement therapy enhances clearance of chylomicron remnants in patients with hypothyroidism.	to further confirm the benefit of replacement therapy in terms of risk for coronary artery disease, we evaluated the effect of t4 on postprandial lipoproteins in patients with hypothyroidism. nine normolipidemic patients (aged 62.75+/-7.6 yr) with tsh of 32.2+/-13.2 mu/l and free t4 of 0.66+/-0.17 ng/ml were treated with t4 (50-100 microg/day) for at least 4 months. the behavior of postprandial lipoproteins was assessed before and during treatment by determining retinyl palmitate levels in the t ...	1999	10404832
sulfation of thyroid hormone by estrogen sulfotransferase.	sulfation is one of the pathways by which thyroid hormone is inactivated. iodothyronine sulfate concentrations are very high in human fetal blood and amniotic fluid, suggesting important production of these conjugates in utero. human estrogen sulfotransferase (sult1e1) is expressed among other tissues in the uterus. here we demonstrate for the first time that sult1e1 catalyzes the facile sulfation of the prohormone t4, the active hormone t3 and the metabolites rt3 and 3,3'-diiodothyronine (3,3'- ...	1999	10404840
performance and physiological variables in broiler chicken lines differing in susceptibility to the ascites syndrome: 2. effect of ambient temperature on partial efficiencies of protein and fat retention and plasma hormone concentrations.	1. male broilers of 5 genetic stocks (a, b, c, d and e), selected in different ways for fast growth and low food conversion rate (fcr) and differing in sensitivity to ascites, were subjected to 2 different ambient temperature (ta) step-down programmes: normal (ht) and low (lt) ta. 2. ascites incidence was followed daily. growth and food intake were measured weekly. heat production (hp), oxygen consumption (oxc) and energy- metabolism parameters were calculated according to the comparative slaugh ...	1999	10405051
comparison of the assembly of the bacteriophage t4 clamp loader complex (gp44/62) expressed in a cis versus trans genomic configuration.	proper formation of the bacteriophage t4 dna polymerase holoenzyme requires a wide spectrum of protein-protein and protein-dna interactions among the dna polymerase gp43, the sliding clamp gp45, and gp44/62, the clamp loader complex (clc). the 44 and 62 proteins associate to form a tight complex maintained in a 4:1 ratio. the 44 and 62 genes are adjacent to each other on the t4 genome, are cotranscribed, and are translationally coupled. it has been suggested that translational coupling may play ...	1999	10405357
fluorescent oligonucleotide probes for clinical and environmental detection of acanthamoeba and the t4 18s rrna gene sequence type.	the first genus- and subgenus-specific fluorescent oligonucleotide probes for in situ staining of acanthamoeba are described. sequences of these phylogeny-based probes complement the 18s rrna and the gene encoding it (18s rdna). the genus-specific probe (gsp) is a fluorescein-labeled 22-mer specific for acanthamoeba as shown here by its hybridization to growing trophozoites of all 12 known acanthamoeba 18s rdna sequence types and by its failure to hybridize with amoebae of two other genera (hart ...	1999	10405422
evaluation of thyroid function in north indians with alopecia areata: response to intravenous injection of 100 micrograms thyrotropin releasing hormone (trh).	there is a lack of agreement on the overall prevalence of thyroid disease and thyroid function abnormalities in alopecia areata. only one study is available from the indian subcontinent. all patients with alopecia areata attending a dermatology outpatient clinic between 1983 and 1997 were screened for the presence of clinical thyroid disease. sixty-two consecutive patients during the year 1994 were evaluated in detail for thyroid functions by measuring t3, t4, tsh levels and testing for antithyr ...	1999	10405476
the efficacy of cryosurgical ablation of prostate cancer: the university of california, san francisco experience.	we analyze biopsy and prostate specific antigen (psa) results following cryosurgery for patients with clinically localized prostate cancer.	1999	10411051
influence of specific mutations on the thermal stability of the td group i intron in vitro and on its splicing efficiency in vivo: a comparative study.	group i introns constitute excellent systems for analyzing the relationship between rna tertiary folding and catalysis. within a hierarchical framework interpretation of rna folding, secondary structure motifs subtend rna three-dimensional (3d) architecture. thus, mutations in two-dimensional motifs are expected to have effects different from those disrupting 3d contacts. using uv spectroscopy, we have studied the influence of nucleotide substitutions, in both secondary and tertiary structure el ...	1999	10411138
effect of low-dose methyl prednisolone on serum cytokine levels following extracorporeal circulation.	the systemic inflammatory response to cardiopulmonary bypass (cpb) is associated with increased production of cytokines. this systemic inflammatory response characterized by the activation of interleukin-6 (il-6) and interleukin-8 (il-8) during and after cpb is well documented. a prospective, randomized, double-blind study was performed so as to understand the effects of low-dose methyl prednisolone sodium succinate (mpss) on the circulating levels of serum cytokines and clinical outcome. twenty ...	1999	10411250
regulation of thyroid hormone metabolism during fetal development.	compared with adults, plasma t3 concentrations in the human fetus are decreased, whereas levels of rt3 and the different iodothyronine sulfates, t4s, t3s, rt3s and 3,3'-t2s, are increased. the low t3 and high rt3 concentrations reflect the preponderance of inner ring versus outer ring deiodinase activity due to high type iii iodothyronine deiodinase (d3) expression in fetal tissues, such as liver and brain, the placenta, and perhaps also the uterus, in combination with still incomplete expressio ...	1999	10411318
local activation and inactivation of thyroid hormones: the deiodinase family.	tissue-specific activation and inactivation of ligands of nuclear receptors which belong to the steroid retinoid-thyroid hormone superfamily of transcription factors represents an important principle of development- and tissue-specific local modulation of hormone action. recently, several enzyme families have been identified which act as 'guardians of the gate' of ligand-activated transcription modulation. three monodeiodinase isoenzymes which are involved in activation the 'prohormone' l-thyrox ...	1999	10411325
[outcome after resection of extensive oropharyngeal carcinomas and defect coverage by microvascular anastomosis of a radialis flap].	extensive tumors of the oropharynx require an open approach and plastic reconstruction for good oncologic and functional results.	1999	10412135
[intraoral cancer in the county of copenhagen].	a retrospective study of patients treated for intra-oral squamous cell carcinoma in copenhagen county is reported. the material included 156 patients, 66 females and 90 males. age at diagnosis varied between 35 and 95 years with a mean of 65 years. mean age of females/males was 70/62 years. twelve therapeutic modalities were performed; surgery, radiotherapy, chemotherapy and different combinations of these. five year disease specific survival related to tumours' t-classification was 84% for t1, ...	1999	10412321
cluster versus conventional immunotherapy in patients allergic to dermatophagoides pteronyssinus: a controlled study of in vivo and in vitro parameters.	we designed a cluster schedule of immunotherapy for patients allergic to dermatophagoides pteronyssinus which showed good safety and clinical efficacy. here we compare the in vivo and in vitro changes with those of a conventional schedule in a controlled trial. sixty-three patients were randomized as follows: 29 were treated with the cluster schedule, 15 with a conventional schedule and 19 received no immunotherapy. a standardized extract was used. changes in in vivo parameters (skin prick test ...	1999	10412676
dermatophagoides pteronyssinus cluster immunotherapy. a controlled trial of safety and clinical efficacy.	we designed a cluster schedule of immunotherapy for patients allergic to dermatophagoides pteronyssinus to reach the maximal recommended dose in 7 weeks. we compared its safety and clinical efficacy with those of a conventional schedule in a controlled trial. sixty-three patients were randomized as follows: 29 were treated with the cluster schedule, 15 with a conventional schedule and 19 without immunotherapy. a standardized extract was used. clinical efficacy was measured by visual analog scale ...	1999	10412677
an evaluation of the carcinogenic potential of the herbicide alachlor to man.	chronic bioassays have revealed that alachlor caused nasal, thyroid, and stomach tumours in rats but was not carcinogenic in mice. significant increases in thyroid and stomach tumours were observed only at doses that exceeded the maximum tolerated dose (mtd). while nasal tumours were found at doses below the mtd, they were small and benign in nature. this publication describes the work undertaken by monsanto to understand the carcinogenic mode of action of alachlor in the rat and to investigate ...	1999	10413243
synthesis and nucleosome structure of dna containing a uv photoproduct at a specific site.	a strategy was developed to assemble nucleosomes specifically damaged at only one site and one structural orientation. the most prevalent uv photoproduct, a cis-syn cyclobutane thymine dimer (cs ctd), was chemically synthesized and incorporated into a 30 base oligonucleotide harboring the glucocorticoid hormone response element. this oligonucleotide was assembled into a 165 base pair double stranded dna molecule with nucleosome positioning elements on each side of the cs ctd-containing insert. p ...	1999	10413526
graves' disease during pregnancy--results of antithyroid drug therapy.	to assess the maternal and fetal outcome of pregnancies in thyrotoxic women.	1999	10414160
role of preoperative chemoradiation in the management of upper third thoracic esophageal squamous cell carcinoma.	the treatment of upper thoracic esophageal cancer remains challenging, and combined treatment is necessary to improve the outcome. we conducted preoperative chemoradiation therapy (crt) for potential t4 tumors located in the upper third esophagus and carried out a retrospective study to elucidate the role of preoperative crt.	1999	10414703
disruption of male sex hormones with regard to pesticides: pathophysiological and regulatory aspects.	several pesticides used as herbicides, insecticides and fungicides are known to be endocrine disrupting chemicals (edcs). in three pair-matched studies we found changes in sex hormone concentrations and t-lymphocytes in relation to acute and chronic pesticide exposure. after acute exposure, 1 day later the concentrations of testosterone and especially estradiol decreased. t4- and t8-lymphocytes slightly increased. effects of chronic occupational pesticide exposure were expressed by a higher leve ...	1999	10414800
sensitivity of thyroid gland growth to thyroid stimulating hormone (tsh) in rats treated with antithyroid drugs.	antithyroid drugs and phenobarbital (pb) have been shown to promote thyroid tumors in rats. it has been proposed that increased thyroid-stimulating hormone (tsh) mediates the thyroid tumor-promoting effect of antithyroid drugs and pb, and is increased because of decreased thyroxine (t4) concentration. however, pb is much less effective than antithyroid drugs at increasing tsh. it has been proposed that small increases in serum tsh produced by pb treatment is sufficient to promote thyroid tumors. ...	1999	10416271
gene 61.3 of bacteriophage t4 is the spackle gene.	the bacteriophage t4 e gene encodes lysozyme (e-lysozyme), which releases progeny phage after normal infection of escherichia coli cells. a mutation in the spackle gene suppresses the defect in e-lysozyme (emrich, 1968). the spackle gene was mapped between genes 41 and 61, but its precise location has not previously been determined. in the current study, we constructed an amber mutant of gene 61.3, amst14, by site-directed mutagenesis. the gene 61.3 mutant shares phenotypes with spackle mutants: ...	1999	10417260
the influence of supplements of selenite, selenate and selenium yeast on the selenium status of dairy heifers.	the aim of the study was to define possible differences between selenite, selenate and selenium yeast on various aspects of selenium status in growing cattle. twenty-four swedish red and white dairy heifers were fed no supplementary selenium for 6 months. the basic diet contained 0.026 mg selenium/kg feed dry matter (dm). after the depletion period the animals were divided into 4 groups; group i-iii received 2 mg additional selenium daily as sodium selenite, sodium selenate, and a selenium yeast ...	1999	10418193
new classification of stage iv squamous cell carcinoma of the oropharynx.	the recent progress in reconstructive surgery for the treatment of head and neck carcinomas has made it possible to radically resect cancers. however. the choice of treatment for oropharyngeal carcinoma is rather difficult. radical treatment sometimes results in severe complications, suggesting that some modes of treatment might reduce the quality of life. the 5-year survival rate of patients with stage iv oropharyngeal carcinoma is still very poor. it is necessary to re-classify stage iv squamo ...	1999	10419040
histopathological analysis of apoptosis, and expression of p53, bcl-2, bax, and ki-67 in laryngeal squamous cell carcinomas and dysplasia.	the growth of neoplasia is determined by the proliferation and loss of cells. the purpose of this study is to determine the frequency of apoptosis in laryngeal carcinomas and to examine its relationship to the pathological parameters, including ki-67 expression, and to expression of p53, bcl-2, and bax protein. the materials are 67 cases of laryngeal squamous cell carcinomas (sccs) and 22 cases of squamous dysplasia using biopsy and surgery specimens. apoptotic cells were determined by the modif ...	1999	10419041
[constitutive inhibition of dna degradation due to the enzyme recbcd in the radiation-resistant escherichia coli k-12 mutant gam(r)444].	exonucleolytic degradation of [3]h-labeled dna was examined in partially purified fractions of lysates obtained from nonirradiated recbcd enzyme-containing cells of escherichia coli and in the radiation-resistant mutant gamr444. the degradative activity was shown to be lowered in these cells to the same extent as in the recbc mutant. the efficiency of plating of the mutant phage t4 2-, dna of which can be degraded by exonuclease v, was 400-fold higher on the strain gamr444 than on the wild-type ...	1999	10420267
surgical results of pancreaticoduodenectomy for carcinoma of the distal third of the stomach.	pancreaticoduodenectomy (pd) was performed as a radical operation on 10 patients who had stage iii or iv carcinoma of the distal third of the stomach which invaded the pancreas head (t4) or had level 3 lymph node metastasis. the surgical results of the pd were compared with those of 69 patients treated with subtotal gastrectomy (sg). although the postoperative morbidity was higher (70%) in the pd group than in the sg group (32%), no hospital death occurred. the overall postoperative survival pro ...	1999	10421012
tumor downstaging and sphincter preservation with preoperative chemoradiation in locally advanced rectal cancer: the m. d. anderson cancer center experience.	to evaluate the rates of tumor downstaging after preoperative chemoradiation for locally advanced rectal cancer.	1999	10421535
[anesthesia in geriatric patients. the determination of physiological variables for cognitive function in geriatric patients after regional or general anesthesia].	the aim of the present study was to show the influence of the parameters of gas exchange (arterial oxygen pressure pao2, arterial oxygen saturation sato2) and haemodynamics (arterial systolic and mean blood pressure rrs and map) on the restitution of cognitive functions in geriatric patients scheduled for elective hip arthroplasty.	1999	10421921
[thermostability of bacteriophage t4 fibritin and its deletion mutants].	the thermal stability of a series of recently obtained mutants of fibritin from bacteriophage t4 (a superhelical fibrous homotrimer with parallel-packed subunits each containing 486 amino acid residues) progressively truncated from the subunit n-end was studied during incubation at 40-90 degrees c in the presence of a surfactant (2% sds). the mutant fibritins, g, b, c, and e, contained 443, 276, 231, and 120 amino acid residues, respectively. one more truncated mutant (fibritin s1, 108 amino aci ...	1999	10422591
neuropeptide y-induced contraction is mediated by neuropeptide y y2 and y4 receptors in the rat colon.	ascending and descending segments of the rat colon were studied to analyze their contractile responses to neuropeptide y and related peptides. these responses are (a) completely eliminated by tetrodotoxin (1 microm), (b) reduced to a variable extent (20 to 60%) by atropine (1 microm) and (c) not modified by indomethacin, diphenhydramine or methysergide. the order of potency of agonists for peptides related to neuropeptide y was as follows: human pancreatic polypeptide = rat pancreatic polypeptid ...	1999	10422644
positional preference of proline in alpha-helices.	conformational free energy calculations have been carried out for proline-containing alanine-based pentadecapeptides with the sequence ac-(ala)n-pro-(ala)m-nhme, where n + m = 14, to figure out the positional preference of proline in alpha-helices. the relative free energy of each peptide was calculated by subtracting the free energy of the extended conformation from that of the alpha-helical one, which is used here as a measure of preference. the highest propensity is found for the peptide with ...	1999	10422838
role of active site residues in the glycosylase step of t4 endonuclease v. computer simulation studies on ionization states.	t4 endonuclease v (endov) is a base excision repair enzyme that removes thymine dimers (td) from damaged dna. to elucidate the role of the active site residues in catalysis, their pk(a)'s were evaluated using the semimicroscopic version of the protein dipoles-langevin dipoles method (pdld/s). contributions of different effects to the pk(a) such as charge-charge interactions, conformational rearrangement, protein relaxation, and dna binding were analyzed in detail. charging of the active site res ...	1999	10423235
thermodynamics of stabilization of rna pseudoknots by cobalt(iii) hexaammine.	equilibrium unfolding (folding) studies reveal that the autoregulatory rna pseudoknots derived from the bacteriophage t2 and t4 gene 32 mrnas exhibit significant stabilization by increasing concentrations of divalent metal ions in solution. in this report, the apparent affinities of exchange inert trivalent co(nh(3))(3+)(6) have been determined, relative to divalent mg(2+), for the folded, partially folded (k(f)), and fully unfolded (k(u)) conformations of these molecules. a general nonspecific, ...	1999	10423552
effect of lung volume reduction surgery on bony thorax configuration in severe copd.	hyperinflation in patients with severe copd is associated with an increased anteroposterior (ap) rib cage diameter. we sought to determine whether bilateral lung volume reduction surgery (lvrs) affects bony thorax configuration.	1999	10424500
the carboxy-terminal domain initiates trimerization of bacteriophage t4 fibritin.	bacteriophage t4 fibritin is a triple-stranded, parallel, segmented alpha-helical coiled-coil protein. earlier we showed that the c-terminal globular domain (foldon) of fibritin is essential for correct trimerization and folding of the protein. we constructed the chimerical fusion protein w31 in which the fibritin foldon sequence is followed by the small globular non-alpha-helical protein gp31 of the t4 phage. we showed that the foldon is capable of trimerization in the absence of the coiled-coi ...	1999	10424907
[poems syndrome. report of a case].	poems syndrome is a multisystem disorder with signs such as peripheral neuropathy, organomegaly, endocrinopathy, monoclonal protein, skin lesions, papilledema, and increased cerebrospinal fluid proteins, which can also evolve with renal and cardiac affection. it is considered a result of a plasma cell cyscrasia with the production of a monoclonal protein. a 46-year old man was seen as an outpatient referring progressive weakness of legs and arms, fever, impotence, inguinal and cervical lymphaden ...	1999	10425826
effect of nicotine on type 2 deiodinase activity in cultured rat glial cells.	intracellular generation of triiodothyronine (t3) from thyroxine (t4) by type 2 deiodinase (d2) in the mammalian brain, plays a key role in thyroid hormone action. the presence of d2 in rat astrocytes suggests the importance of glial cells in the regulation of intracellular t3 levels in the rat central nervous system (cns). to analyze further the factors that regulate d2 activity in the cns, we investigated the effects of nicotine and of mecamylamine, which inhibits the binding of nicotine with ...	1999	10426574
long-term treatment with bromocriptine of a plurihormonal pituitary adenoma secreting thyrotropin, growth hormone and prolactin.	a 48-year-old female presented with acromegaly, amenorrhea and hyperthyroidism associated with high serum free t4 levels and measurable tsh concentrations. the administration of ghrh induced significant increases in gh, prl and tsh. conversely, intravenous infusion of dopamine or oral administration of bromocriptine effectively inhibited gh, prl and tsh secretion. serum alpha-subunit levels were neither affected by ghrh, dopamine nor bromocriptine. transsphenoidal surgery was performed and immun ...	1999	10426581
a case of graves' disease associated with autoimmune hepatitis and mixed connective tissue disease.	the patient was a woman of forty-eight. liver dysfunction was pointed out at the age of forty-five. she was admitted to hospital because of her hyperthyroidism. her palmar skin was wet and her fingers were swollen like sausages. she had a diffuse and elastic hard goiter with a rough surface. the serum levels of free t3 (9.6 pg/ml) and free t4 (3.76 ng/dl) were high and that of tsh (0.11 microu/ml) was low. the activity of tsh-binding inhibitory immunoglobulin (tbii) was 89%. the uptake rate of 1 ...	1999	10426583
suppressive therapy with levothyroxine for euthyroid diffuse and nodular goiter.	in this study, 35 patients with euthyroid diffuse goiter and 35 patients with euthyroid nodular goiter were treated with levothyroxine (l-t4) for six months. the aim was to evaluate the efficacy of treatment on thyroid and nodule volumes and to evaluate the correlation between volume changes and thyroglobulin levels. serum thyroid hormones, tsh, thyroglobulin, thyroid and nodule volumes were measured at the initial visit and after 6 months. radioactive iodine uptakes of the thyroid gland were ev ...	1999	10426591
regulation of neuropeptide y release by neuropeptide y receptor ligands and calcium channel antagonists in hypothalamic slices.	neuropeptide y (npy) is an important regulator of energy balance in mammals through its orexigenic, antithermogenic, and insulin secretagogue actions. we investigated the regulation of endogenous npy release from rat hypothalamic slices by npy receptor ligands and calcium channel antagonists. high-potassium stimulation (60 mm) of the slices produced a calcium-dependent threefold increase in npy release above basal release. the y2 receptor agonists npy(13-36) and n-acetyl[leu28,leu31]npy(24-36), ...	1999	10428060
simultaneous interactions of bacteriophage t4 dna replication proteins gp59 and gp32 with single-stranded (ss) dna. co-modulation of ssdna binding activities in a dna helicase assembly intermediate.	the t4 gp59 protein is the major accessory protein of the phage's replicative dna helicase, gp41. gp59 helps load gp41 at dna replication forks by promoting its assembly onto single-stranded (ss) dna covered with cooperatively bound molecules of gp32, the t4 single-strand dna binding protein (ssb). a gp59-gp32-ssdna ternary complex is an obligatory intermediate in this helicase loading mechanism. here, we characterize the properties of gp59-gp32-ssdna complexes and reveal some of the biochemical ...	1999	10428868
persistent infantile hypothyroidism attributable to thyroxine-binding globulin deficiency.	an infant diagnosed with thyroid-binding globulin (tbg) deficiency after newborn screening demonstrated persistent elevation of thyroid-stimulating hormone (tsh) and abnormally low free thyroxine (ft4) levels. treatment with thyroxine (t4) normalized ft4 and tsh levels during the first 5 years of life, but withdrawal of t4 supplementation at that time was associated with return of hyperthyrotropinemic hypothyroidism. to our knowledge, this patient is the first reported case of tbg deficiency-ass ...	1999	10429016
[total pelvic exenteration and rectal cancer. apropos of 20 cases].	the report of a series of 20 patients with the aim of trying to specify the implications of pelvic exenteration for rectal cancer.	1999	10429298
the impact of nutritional status on the prognoses of patients with advanced head and neck cancer.	malnutrition has been recognized as a poor prognostic indicator for cancer treatment-related morbidity and mortality in general, and it is reported to affect 30-50% of all patients with head and neck cancer. in this study, the correlation of nutritional status with 3-year survival was studied prospectively in 64 patients with t2-t4 carcinomas of the head and neck who were treated surgically with curative intent; the surgery was often followed by radiotherapy.	1999	10430262
radical surgery for gallbladder cancer. results of the french surgical association survey.	gallbladder carcinoma is a highly lethal disease. the advantages of radical surgery remain controversial. the authors' objective was to evaluate the effectiveness of an aggressive approach to gallbladder carcinoma on long-term survival.	1999	10430296
genetic analysis of the bacteriophage t4-encoded cochaperonin gp31.	previous genetic and biochemical analyses have established that the bacteriophage t4-encoded gp31 is a cochaperonin that interacts with escherichia coli's groel to ensure the timely and accurate folding of gp23, the bacteriophage-encoded major capsid protein. the heptameric gp31 cochaperonin, like the e. coli groes cochaperonin, interacts with groel primarily through its unstructured mobile loop segment. upon binding to groel, the mobile loop adopts a structured, beta-hairpin turn. in this artic ...	1999	10430575
structural basis of the conversion of t4 lysozyme into a transglycosidase by reengineering the active site.	in contrast to hen egg-white lysozyme, which retains the beta-configuration of the substrate in the product, t4 lysozyme (t4l) is an inverting glycosidase. the substitution thr-26 --> his, however, converts t4l from an inverting to a retaining enzyme. it is shown here that the thr-26 --> his mutant is also a transglycosidase. indeed, the transglycosylation reaction can be more effective than hydrolysis. in contrast, wild-type t4l has no detectable transglycosidase activity. the results support t ...	1999	10430876
laparoscopic evaluation of the risk of locoregional recurrence after radical surgery for gastric cancer.	we studied the diagnostic utility of laparoscopy in patients who underwent absolute radical surgery (r0) for gastric adenocarcinoma and who were at risk for locoregional recurrence. risk was considered to exist in patients with t3-t4 stage tumors (iuac tnm classification) that invaded the serosa or affect nearby organs. these patients are candidates for clinical trials involving multimodal therapies, especially with neoadjuvant or intraperitoneal chemotherapy.	1999	10431090
interaction of beta-l-adenosine-5'-triphosphate (l-atp) with human deoxycytidine kinase, human dna primase and t4 dna ligase: does the chance direct enzymatic enantioselectivity?	we demonstrate that l-atp: 1) as well as its natural d-enantiomer, acts as a phosphate donor in the reaction catalysed by human deoxycytidine kinase; 2) inhibits human dna-primase and the atp-dependent t4 dna ligase. thus, the lack of enantioselectivity of the enzymes is more frequent than it was believed a few years ago and we suggest that it would depend on chance more than on an evolutionary strategy.	1999	10432697
pretherapeutic laparoscopic staging in advanced gastric carcinoma.	direct visualization of the abdominal cavity by laparoscopy prior to multimodal treatment may be capable of improving the diagnostic precision of gastric cancer staging. the aim of this study was to evaluate whether diagnostic laparoscopy can influence treatment strategies in gastric cancer staged t3 and t4 by preoperative diagnostic tests.	1999	10433041
escape from the acute wolff-chaikoff effect is associated with a decrease in thyroid sodium/iodide symporter messenger ribonucleic acid and protein.	in 1948, wolff and chaikoff reported that organic binding of iodide in the thyroid was decreased when plasma iodide levels were elevated (acute wolff-chaikoff effect), and that adaptation or escape from the acute effect occurred in approximately 2 days, in the presence of continued high plasma iodide concentrations. we later demonstrated that the escape is attributable to a decrease in iodide transport into the thyroid, lowering the intrathyroidal iodine content below a critical inhibitory thres ...	1999	10433193
cloning and characterization of type iii iodothyronine deiodinase from the fish oreochromis niloticus.	type iii iodothyronine deiodinase (d3) catalyzes the inner ring deiodination (ird) of t4 and t3 to the inactive metabolites rt3 and 3,3'-diiodothyronine (3,3'-t2), respectively. here we describe the cloning and characterization of complementary dna (cdna) coding for d3 in fish (oreochromis niloticus, tilapia). this cdna contains 1478 nucleotides and codes for a protein of 267 amino acids, including a putative selenocysteine (sec) residue, encoded by a tga triplet, at position 131. the deduced am ...	1999	10433225
mandibular articular disk position changes during herbst treatment: a prospective longitudinal mri study.	the aim of this prospective longitudinal study of 15 consecutively treated class ii malocclusions was to assess any possible changes in the relative position of the articular disk to the condyle during different phases of herbst therapy. using a disk position index, parasagittal mris (central, medial and lateral slices) of the right and left tmj were analyzed at five occasions: before herbst treatment (t1), at start of treatment when the appliance was placed (t2), after 6 weeks of treatment (t3) ...	1999	10434095
[the hyoid bone in reconstructive laryngeal and cervical surgery].	the hyoid bone, with its complex system of connected muscles, ligaments and membranes, provides a highly resistant anchor point in all cases of larynx tumour surgery requiring a "pexy" to reconstruct the upper respiratory tract. this bone can also be important in reconstructive cervical surgery employing myocutaneous flaps, especially after extensive tissue removal, such as in locally advanced larynx tumours (primary t4 lesions or recurrences after partial or subtotal operations) and some primar ...	1999	10434457
[intramedullary metastasis of small cell lung cancer].	a 64-year-old man complaining of hoarseness received a diagnosis of small cell lung cancer (t4 n3 m1, stage iv) in may 1997. he responded well to chemotherapy and radiotherapy, and afterward underwent brain irradiation for prophylactic purposes. however, on nov. 27 the patient experienced lumbar pain and numbness in his left leg. and was admitted to our hospital on dec. 2 because his symptoms deteriorated to palsy of the left leg. brain computed tomographic scans and lumbar magnetic resonance im ...	1999	10434549
[effects of a 5-day fast on clinical laboratory data from patients with rheumatoid arthritis].	there have been few studies on the effects of a fast on clinical laboratory data in japanese. we studied twelve women with rheumatoid arthritis who were not taking any medicine and stayed in the koda hospital for a diet which lasted 55 days. they basically took a 1200 kcal vegan diet and undertook a 3-5-day fast three times. the clinical laboratory data obtained before and after the second fast (day 27-day 31) were compared. average body weight decreased by 1.5 kg. there were no changes in crp. ...	1999	10434574
[monitoring of treatment in thyroid diseases].	the effectiveness of antithyroid drug treatment of graves' hyperthyroidism is documented by measuring initially free t4 and free t3 and later free t4, free t3 and tsh. an elevated titer of the graves'-specific thyroid stimulating antibodies is not usually rechecked before the end of the antithyroid drug therapy. thyroxine treatment of primary hypothyroidism is controlled by tsh measurements. in patients in whom tsh levels might be affected by drugs or nonthyroid diseases, free t4 is measured in ...	1999	10434779
diagnosis and treatment of the solitary thyroid nodule. results of a european survey.	the optimum diagnostic and therapeutic strategy for the euthyroid patient with a solitary thyroid nodule is still a matter of debate. the aim was to assess the attitudes towards management of such patients in clinical centres throughout europe by means of a questionnaire.	1999	10435062
a third decade's experience with the gastric pull-up operation for hypopharyngeal carcinoma: changing patterns of use.	gastric transposition has been used extensively in this department since 1965 for reconstruction following pharyngolaryngoesophagectomy (plo). a previous report by harrison and thompson in 1986 detailed our experience with 101 cases dating back to 1965. here we review our experience between 1986-1996. medical records of 41 cases were examined. the primary site and stage of tumour and associated lymph nodes, patient demographics, complications, in-patient mortality and survival as demonstrated by ...	1999	10435132
prognostic implications of response to preoperative infusional chemoradiation in locally advanced rectal cancer.	to evaluate the influence of response to preoperative infusional chemoradiation on outcome parameters among patients with locally advanced rectal cancer.	1999	10435807
primary hypothyroidism-associated tsh-secreting pituitary adenoma/hyperplasia presenting as a bleeding nasal mass and extremely elevated tsh level.	a 41-year-old male with primary hypothyroidism and a huge tsh-secreting pituitary tumor presented with a bleeding nasal mass that was initially misdiagnosed as a paraganglioma. other unique features of the case include lack of complaints related to hypothyroidism, an extremely elevated tsh level of 3474 mu/l, and a low prolactin level. the presence of primary hypothyroidism made differentiating tsh-secreting pituitary adenoma from secondary thyrotroph hyperplasia difficult. a low molar ratio of ...	1999	10435850
a comparison of preconstituted, fixed combinations of low-dose glyburide plus metformin versus high-dose glyburide alone in the treatment of type 2 diabetic patients.	in the present study we assessed and compared the effectiveness and safety of preconstituted, fixed, combinations of low-dose glyburide plus metformin with higher-dose glyburide monotherapy in patients with type 2 diabetes. this randomized, double-blind, cross-over study comprised 40 patients. after a 30-day run-in period of dietary treatment, patients received combined glyburide (5, 7.5 or 10 mg/day) and metformin (800, 1,200 or 1,600 mg/day) as preconstitued, fixed combinations, or glyburide a ...	1999	10436254
interaction of tamoxifen with concurrent cytotoxic adjuvant treatment affects lymphocytes and lymphocyte subsets counts in breast cancer patients.	the effects and interaction of endocrine and cytotoxic adjuvant treatment on measures of cellular immunity were assessed in 41 stage i-ii breast cancer patients from international breast cancer study group trials. counts of lymphocytes and lymphocyte subsets [(t, t4, t8, b, natural killer (nk) and activated t (at) cells] were assessed by flow cytometry immediately before adjuvant therapy at baseline and on day 1 of the 3rd cycle. twenty-two patients received cyclophosphamide, methotrexate and 5- ...	1999	10335933
the non-enzymatic microbicidal activity of lysozymes.	t4 lysozyme was thought to destroy bacteria by its muramidase activity. however, we demonstrate here that amphipathic helix stretches in the c-terminus of t4 lysozyme mediate its bactericidal and fungistatic activities. in heat-denatured t4 lysozyme, the enzymatic activity is completely abolished but unexpectedly, the antimicrobial functions remain preserved. small synthetic peptides corresponding to amphipathic c-terminal domains of t4 lysozyme show a microbicidal activity. its membrane disturb ...	1999	10338111
structural characterization of an engineered tandem repeat contrasts the importance of context and sequence in protein folding.	to test a different approach to understanding the relationship between the sequence of part of a protein and its conformation in the overall folded structure, the amino acid sequence corresponding to an alpha-helix of t4 lysozyme was duplicated in tandem. the presence of such a sequence repeat provides the protein with "choices" during folding. the mutant protein folds with almost wild-type stability, is active, and crystallizes in two different space groups, one isomorphous with wild type and t ...	1999	10339544
is it possible to diagnose canine hypothyroidism?	a definitive diagnosis of hypothyroidism can be difficult because of the many clinical abnormalities associated with thyroid hormone deficiency, and the lack of readily available diagnostic tests with high sensitivity and specificity. thyroid function tests should be performed only in dogs with clinical findings consistent with hypothyroidism. measurement of serum total thyroxine (t4) concentration is a useful initial screening test since most hypothyroid dogs have values below the reference ran ...	1999	10340243
hiv pathogenesis: gp120-antibody complexes bind cd4 and kill t4 cells--immunotoxin therapy should prevent the progression of hiv to aids.	current models of the role of human immunodeficiency virus (hiv) in the pathogenesis of acquired immune deficiency syndrome (aids) are inadequate and inconsistent with the literature. this article reviews a wide range of aids research and proposes the first model of hiv pathogenesis that is entirely consistent with the literature. this model is based on antibody-gp120 complex crosslinking of cd4 on the t4 cells. previously unexplained observations embraced by this model include early qualitative ...	1999	10340294
intraoperative pleural lavage in esophageal carcinoma.	cytological examination of intraoperative pleural or peritoneal lavage specimens is useful for predicting outcomes for patients with various carcinomas. there have been few reports regarding cytological examination of pleural lavage fluid in esophageal carcinoma.	1999	10340891
recombinant interferon alpha (rifn-alpha) does not potentiate the effect of iodine excess on the development of thyroid abnormalities in patients with hcv chronic active hepatitis.	to determine whether the administration of pharmacological quantities of iodine during interferon-alpha (rifn-alpha) treatment of chronic viral hepatitis b and c (hcv) would exacerbate the potential adverse effects of rifn alpha on thyroid function.	1999	10341861
kinetics of red blood cell t3 uptake in hypothyroidism with or without hormonal replacement, in the rat.	l-triiodothyronine (l-t3) is taken up and accumulated into red blood cells (rbc) by means of a specific carrier-mediated system. the aim of this study was to evaluate the reactivity of this system in relation to induced alterations in thyroid hormone (th) supply. we investigated the kinetic parameters (vmax, maximal velocity and km, michaelis constant) of washed-rbc l-t3 uptake 1) in thyroidectomized (txt) rats, 2) in txt rats administered with low doses of l-t4 (15 microg/kg/day x 14) to restor ...	1999	10342358
superior sulcus lung tumors: impact of local control on survival.	our goal was to assess patient survival and response to treatment for superior sulcus tumors treated with combined radiation therapy and surgery when possible, or with radiation alone when surgery was not possible.	1999	10343256
massive goitre (struma parenchymatosa) in geese.	in a goose flock consisting of 2300 birds of 6 months of age severe goitre was diagnosed. to the best of our knowledge this is the first report of naturally occurring goitre in geese, which is not related to the feeding of rapeseed meal. the major pathological findings included retarded growth and plumage development, significantly (300%) increased relative thyroid weight, fat accumulation in the mesenteric and abdominal region, and lipid infiltration of liver and kidney cells. subsequent hormon ...	1999	10344082
replication-induced protein synthesis and its importance to proteomics.	replication-induced protein synthesis (rips) can occur following the passage of the replisome due to transcription initiated by rna polymerase in association with: (i) negative supercoiling trailing the replisome / replication fork, (ii) hemimethylation prior to the action of dam methylase, (iii) transient derepression following passage of the replisome/replication fork and prior to renewed synthesis of the repressor gene-product, and (iv) 'sliding clamp' accessory dna-binding proteins binding t ...	1999	10344230
3-amino-5-phenoxythiophenes: syntheses and structure-function studies of a novel class of inhibitors of cellular l-triiodothyronine uptake.	a series of substituted 3-amino-5-phenoxythiophenes was synthesized starting from malodinitrile and carbon disulfide. the resulting dicyanoketenedithiolate reacts via thorpe-dieckmann cyclization with halogen methanes bearing electron-withdrawing groups to give thiophene-2-thiolates, which can be transformed into 3-amino-5-(methylsulfonyl)thiophene-4-carbonitriles. replacement of the methylsulfonyl groups by substituted phenolates provides the substituted 3-amino-5-phenoxythiophenes. some of the ...	1999	10346938
folding and stability of mutant scaffolding proteins defective in p22 capsid assembly.	bacteriophage p22 scaffolding subunits are elongated molecules that interact through their c termini with coat subunits to direct icosahedral capsid assembly. the soluble state of the subunit exhibits a partially folded intermediate during equilibrium unfolding experiments, whose c-terminal domain is unfolded (greene, b., and king, j. (1999) j. biol. chem. 274, 16135-16140). four mutant scaffolding proteins exhibiting temperature-sensitive defects in different stages of particle assembly were pu ...	1999	10347166
role of leptin in women with eating disorders.	this study investigated the role of leptin on eating behavior and reproductive function in eating disorders.	1999	10349581
nmr observation of t-tetrads in a parallel stranded dna quadruplex formed by saccharomyces cerevisiae telomere repeats.	we report here the nmr structure of the dna sequence d-tggtggc containing two repeats of saccharomyces cerevisiae telomere dna which is unique in that it has a single thymine in the repeat sequence and the number of gs can vary from one to three. the structure is a novel quadruplex incor-porating t-tetrads formed by symmetrical pairing of four ts via o4-h3 h-bonds in a plane. this is in contrast to the previous results on other telomeric sequences which contained more than one t in the repeat se ...	1999	10352174
analysis of the cooperative thermal unfolding of the td intron of bacteriophage t4.	the thermal stability of folded transcripts of the td intron of bacteriophage t4 that carried up to three base substitutions was investigated by temperature gradient gel electrophoresis (tgge) and uv melting. the unfolding of this autocatalytic group i intron is endothermic and entropically driven. although the effects of mutations in base pairs follow in most cases the expected order g-c>a-u>g.u>a.c, the extent of global destabilization varies strongly according to the helix in which substituti ...	1999	10352179
immunologic and biochemical factors in hyperemesis gravidarum with or without hyperthyroxinemia.	this study was set up to investigate the relationship between immune process and high levels of human chorionic gonadotropin-beta (betahcg) in hyperemesis patients with or without hyperthyroxinemia.	1999	10352382
[conservative surgical treatment in differentiated thyroid carcinoma].	purpose of the present study was to compare survival of patients affected by differentiated thyroid carcinoma after total and partial thyroidectomy.	1999	10352523
increased serum angiogenin concentration in colorectal cancer is correlated with cancer progression.	we have previously demonstrated that the increased expression of angiogenin (ang) in pancreatic cancer is related to cancer aggressiveness; however, the relationship between ang expression and its clinical relevance in colorectal cancer has not been demonstrated. we therefore investigated the correlation between serum ang (sang) concentration and colorectal cancer progression or the changes in sang concentrations before and after cancer resection. to determination sang concentration by elisa, se ...	1999	10353747
growth, puberty, and endocrine functions in patients with sporadic or familial neurofibromatosis type 1: a longitudinal study.	this study prospectively evaluates parameters of growth, puberty, and attained adult height in children with sporadic or familial occurrence of neurofibromatosis type 1 (nf-1), followed up longitudinally, to define the most important factors affecting these parameters.	1999	10353939
the 0.8% ultraviolet b content of an ultraviolet a sunlamp induces 75% of cyclobutane pyrimidine dimers in human keratinocytes in vitro.	tanning lamps, emitting predominantly ultraviolet (uv) a, are used widely throughout the u.k. and other countries, but little is known about the long-term risks associated with their use, especially with respect to skin cancer. we have exposed normal human epidermal keratinocytes to a commercial tanning lamp and used the comet assay in association with dna repair enzymes t4 endonuclease v and endonuclease iii to investigate the relative yields of directly formed cyclobutane pyrimidine dimers (cp ...	1999	10354066
a multifrequency electron spin resonance study of t4 lysozyme dynamics.	electron spin resonance (esr) spectroscopy at 250 ghz and 9 ghz is utilized to study the dynamics and local structural ordering of a nitroxide-labeled enzyme, t4 lysozyme (ec 3.2.1.17), in aqueous solution from 10 degrees c to 35 degrees c. two separate derivatives, labeled at sites 44 and 69, were analyzed. the 250-ghz esr spectra are well described by a microscopic ordering with macroscopic disordering (momd) model, which includes the influence of the tether connecting the probe to the protein ...	1999	10354455
combined sequential approach in locally advanced breast cancer.	the interaction between primary and adjuvant chemotherapy is a crucial point in the treatment of locally advanced breast cancer.	1999	10355574
strategies for recombinant protein recovery from canolaby precipitation	transgenic plants may prove to be one of the most economical systems for the large-scale production of proteins and peptides. our goal is to develop an approach for protein recovery from canola that will be adaptable to a wide variety of recombinant proteins. for recombinant protein recovery, the two downstream processes considered were extraction of target protein and purification of recombinant protein from host proteins and other impurities. in these experiments, t4 lysozyme has been added to ...	1999	10356267
aqueous two-phase systems containing urea: influence on phase separation and stabilization of protein conformation by phase components.	during recombinant escherichia coli fermentation with high expression levels, inclusion bodies are often formed. aqueous two-phase systems have been used in the presence of urea for the initial recovery steps. to investigate phase behavior of such systems we determined phase diagrams of poly(ethylene glycol) (peg)/sodium sulfate/urea/water and peg/dextran t-500 (dex)/urea/phosphate buffer/water at different concentrations of urea and different molecular weight of peg. peg/na2so4 aqueous two-phas ...	1999	10356268
enhancing or suppressive effects of antibodies on processing of a pathogenic t cell epitope in thyroglobulin.	thyroglobulin (tg)-specific abs occur commonly in thyroid disease, but it is not clear to what extent they affect tg processing and presentation to t cells. here we show that generation of the nondominant pathogenic tg epitope (2549-2560), containing thyroxine (t4) at position 2553 (t4(2553)), is augmented by tg-specific igg mabs that facilitate fcr-mediated internalization of tg. however, other mabs of the same (igg1) subclass enhanced tg uptake by apc but had no effect on the generation of thi ...	1999	10358139
unexpected abundance of self-splicing introns in the genome of bacteriophage twort: introns in multiple genes, a single gene with three introns, and exon skipping by group i ribozymes.	analysis of rna that can be labeled with gtp indicates the existence of group i introns in genes of at least three transcriptional classes in the genome of staphylococcus aureus bacteriophage twort. a single orf of 142 amino acids (orf142) is interrupted by three self-splicing group i introns, providing the first example of a phage gene with multiple intron insertions. twort orf142 is encoded in a message that is abundant 15-20 min after infection and is highly similar to a late gene product (or ...	1999	10359829
mutational analysis of coms: evidence for the interaction of coms and meca in the regulation of competence development in bacillus subtilis.	the development of bacillus subtilis genetic competence is a highly regulated adaptive response to stationary-phase stress. a key step in competence development is the activation of the transcriptional regulator comk, which is required for the expression of genes encoding the products that function in dna uptake. in log-phase cultures, comk is trapped in a complex composed of meca and clpc, in which it is rendered inactive. the coms gene, contained within the srf operon, is induced in response t ...	1999	10361283
effects of pyridostigmine and naloxone on the abnormal tsh response to trh during starvation in humans.	starvation is associated with a blunted tsh response to thyrotropin-releasing hormone (trh) (peak minus baseline < 5 miu/l), despite basal tsh and thyroid hormone levels within the normal range. in light of the inhibitory effect of somatostatin on tsh secretion, we examined whether this condition is caused by an increased hypothalamic somatostatinergic tone in starving subjects. the possible involvement of endogenous opioids in the mechanism underlying the abnormal tsh response to trh was also e ...	1999	10361382
bronchogenic carcinoma in young patients.	some investigators have suggested that lung cancer in young patients has a more aggressive course and poorer prognosis than lung cancer in older patients.	1999	10362088
quetiapine, a novel antipsychotic: experience in elderly patients with psychotic disorders. seroquel trial 48 study group.	this uncontrolled trial examines the safety and effects of quetiapine, a new atypical antipsychotic, in elderly patients with psychotic disorders.	1999	10362435
fold recognition study of alpha3-galactosyltransferase and molecular modeling of the nucleotide sugar-binding domain.	the structure and fold of the enzyme responsible for the biosynthesis of the xenotransplantation antigen, namely pig alpha3 galactosyltransferase, has been studied by means of computational methods. secondary structure predictions indicated that alpha3-galactosyltransferase and related protein family members, including blood group a and b transferases and forssman synthase, are likely to consist of alternating alpha-helices and beta-strands. fold recognition studies predicted that alpha3-galacto ...	1999	10362841
[hypothyroidism in patients treated for laryngeal cancer: preliminary results].	the reported incidence of hypothyroidism following surgery and/or irradiation for laryngeal cancer varies widely and the condition often is misdiagnosed. this study examines the incidence of thyroid dysfunction in patients with laryngeal cancer.	1999	10362864
vector analysis of visual evoked potentials elicited by pattern reversal and photic stimuli.	the 2d veps to pattern reversal (pr) and led goggle were studied in order to obtain a stable parameter for the functional assessing of posterior visual pathways regardless of the stimulus type used.	1999	10363760
effects of experimental hypothyroidism on 5-ht1a, 5-ht2a receptors, 5-ht uptake sites and tryptophan hydroxylase activity in mature rat brain1.	the study was aimed at investigating the repercussions of deficiency in thyroid function with and without thyroid hormone (th) replacement on the neurochemical entities which underly serotonin (5-ht) neutrotransmission, namely 5-ht1a, 5-ht2a receptors, 5-ht transporter and tryptophan hydroxylase (tph) in the mature brain. surgically thyroidectomized male wistar rats received: (1) an iodine-free diet to produce severe hypothyroidism; (2) hormonal replacement with 15 microgram/kg/day of thyroxine ...	1999	10364698
thyroid hormones regulate the expression of somatostatin receptor subtypes in the rat pituitary.	circulating tsh levels are increased in hypothyroidism and suppressed in hyperthyroidism. on the other hand, the hypothalamic hormone somatostatin suppresses basal and trh-induced tsh release, an effect which is enhanced by thyroid hormones. to investigate whether the effects of thyroid hormones on tsh secretion may be mediated in part through alterations in the gene expression of pituitary somatostatin receptors (sstr), 3-week-old male sprague-dawley rats were rendered hypothyroid with antithyr ...	1999	10364699