Publications

effect of von willebrand factor and its proteolytic fragments on kinetics of interaction between the light and heavy chains of human factor viii.	it was previously shown that vwf increases the rate of divalent cation-mediated fviii reconstitution from isolated light chain (lch) and heavy chain (hch) subunits. we examined the effect of vwf on kinetic parameters for interaction between lch and hch in the presence of ca2+ and mn2+ ions, the most effective mediators of fviii reconstitution from isolated subunits, and determined the minimal structural portion of vwf able to enhance fviii formation. we found that affinity (kd) for lch/hch bindi ...	1999	10605949
new reporter cell lines to study macrophage-tropic hiv envelope protein-mediated cell-cell fusion.	the infection of human cells by hiv-1 virus can be mimicked by a fusion process between cells expressing the hiv envelope protein (env) and cells expressing both human cd4 (hucd4) and appropriate human chemokine receptors. in this study, a macrophage-tropic (m-tropic) hiv cell-cell fusion assay was established that utilized hucd4, human ccr5 (huccr5), and hiv adagpl60 as fusion components and a gal4/vp16-activated luciferase as a reporter system. by combining cho cells expressing hucd4 and huccr ...	1999	10606089
thyrotoxicosis, unstable angina and normal coronary angiogram.	it is well known that thyrotoxicosis may elicit acute myocardial ischemia even in patients with angiographically normal coronary vessels. the involved mechanisms are not clearly defined although some hypothesis have been suggested. we report a case of a 54-year-old woman affected by graves' disease with thyrotoxicosis which was referred to our institute because of unstable angina. during hospitalization a two dimensional echocardiogram, performed during chest discomfort, showed left ventricular ...	1999	10609135
iodine and brain development.	the development of the brain is critically dependent on an adequate supply of iodine. iodine is an integral part of thyroid hormone, which acts on brain development by regulating the expression of target genes. the active thyroid hormone, t3, is generated in part in the thyroid gland, but about 80% of t3 in brain is formed locally from t4 deiodination mainly by the action of a specific iodothyronine deiodinase. this enzyme is highly expressed in astrocytes, which take up t4 from the blood and de ...	1999	10609893
toxicity and metabolism of malachite green and leucomalachite green during short-term feeding to fischer 344 rats and b6c3f1 mice.	malachite green, an n-methylated diaminotriphenylmethane dye, has been widely used as an antifungal agent in commercial fish hatcheries. malachite green is reduced to and persists as leucomalachite green in the tissues of fish. female and male b6c3f1 mice and fischer 344 rats were fed up to 1200 ppm malachite green or 1160 ppm leucomalachite green for 28 days to determine the toxicity and metabolism of the dyes. apoptosis in the transitional epithelium of the urinary bladder occurred in all mice ...	1999	10682936
[concentration of t3 and t4 in blood of non-irradiated and irradiated with different doses rats fasted for two days beforehand].	there were no changes in concentration of t3 and t4 in blood of the rats that were irradiated with a dose of 0.5, 1, 2, 4 and 6 gy in comparison with non-irradiated rats, if the animals were not fed for two days before decapitation. this suggests that the effect of ionizing radiation on thyroid function is mediated by anorexia syndrome. the decrease in concentration of t4 after exposure to 8 gy cannot be explained by postradiative anorexia and most likely is connected with starting enterotoxemy ...	1999	10689431
free thyroxine and thyroid-stimulating hormone levels in patients with seasonal affective disorder and matched controls.	seasonal affective disorder (sad) is characterized by recurrent episodes of depression in the fall and winter that alternate with nondepressed periods in the spring and summer. because some symptoms of sad, such as decreased energy and weight gain, also occur in hypothyroidism, it is possible that individuals with sad have a subtle decrease in thyroid function. to test this hypothesis, we studied blood levels of free thyroxine (t4) and thyroid-stimulating hormone (tsh) in sad patients and matche ...	1999	10701477
overexpression of mps antigens by squamous cell carcinomas of the head and neck: immunohistochemical and serological correlation with fdg positron emission tomography.	survival from advanced primary or recurrent squamous cell carcinoma (scc) of the head and neck (h&n) is poor. more accurate detection of primary tumors and recurrence may provide ways to improve survival. no standard serum tumor marker is routinely used for surveillance of scc-h&n. in this paper, we evaluated the performance characteristics of the mps-h tumor marker test for the quantitative measurement of "mps-h" heat-generated immunoreactive proteins and assessed the clinical utility of this m ...	1999	10697607
nodular goiter and thyroid carcinoma in children and adolescents in a moderate endemic area (lower silesia-sudeten endemia) in the last twelve years.	although thyroid carcinoma is more common in the adult population, the risk of a nodule being malignant is greater in children. the aim of our present investigation was to ascertain the percentage of malignancy in nodular goiter observed in patients from the lower silesia region in the last 12 years. the examination included 60 children (12 boys and 48 girls) treated in our clinic from 1987 to june 1998. age varied from 7 to 18 years (mean 14.8 +/- 2.4), most of them in the age group between 13 ...	1999	10703536
clinical results of total cystectomy for 92 patients with bladder cancer.	the survival rate of 92 patients with primary bladder cancer who had undergone total cystectomy during a 13-year period from 1984 to 1996 was examined. the mean follow-up period was 1,886 days. the 5-year survival rate was 67.9% and the 10-year survival rate was 55.1%. when survival rates were compared pathohistologically, with 81 patients with transitional cell carcinoma divided into two groups, a high-stage group including t3 and t4 patients and a low-stage group with all other patients, the c ...	1999	10730083
a de novo l330s point mutation in thyroid hormone receptor beta gene in a thai female with resistance to thyroid hormone.	in the present study, we report a thai female with a de novo mutation in thyroid hormone receptor-beta (trbeta) gene causing resistance to thyroid hormone (rth). the patient was a 19 year-old woman who presented with goiter for 1 year. except for tachycardia she had no signs of thyrotoxicosis. previously she was treated with propylthiouracil based on the diagnosis of thyrotoxicosis for 9 months and her goiter became more enlarged. the patient was the only child of the family. her parents were al ...	1999	10724359
proposal for modification of the tnm staging classification for cancer of the oral cavity. dosak.	the prognostic value of the tnm and ptnm classifications currently used for tumours of the oral cavity is unsatisfactory. a better classification should be aimed at as today's definition of t4 leads to overclassification of many tumours and today's definition of n3 results in too few lymph nodes in this group. until 1987 the grade of fixation of lymph-nodes was part of the n-classification for oral cancer as it is currently used in the n-classification of breast cancer.	1999	10717829
thyroid function in healthy thai neonates.	to construct a normative data for serum thyroxine (t4), free t4 (ft4), triiodothyronine (t3) and thyrotropin (tsh) in thai neonates.	1999	10730513
lessons from the use of aromatase inhibitors in the neoadjuvant setting.	postmenopausal patients with oestrogen receptor-positive locally advanced t4b, n0-1, m0 and large operable breast cancers t2>3 cm, t3, t4, n0-1 and m0 have been treated with 2.5 mg letrozole (12 patients), 10 mg letrozole (12 patients), 1 or 10 mg anastrozole (24 patients) and 20 mg tamoxifen (65 patients). there was no apparent difference in response rate between 2.5 and 10 mg letrozole. only 17 patients with anastrozole have so far completed the 3-month treatment period. median clinical, mammo ...	1999	10731113
assessment of quality of life in recently post-menopausal women on dopaminergic therapy for pathological hyperprolactinaemia.	psychological distress has been reported in pre-menopausal hyperprolactinaemic women. the aim of this study was to assess quality of life in a group of recently post-menopausal women with a long-term history of hyperprolactinaemia.	1999	10731741
regulation of thyroid hormones on the production of testosterone in rats.	the effects of a thyroidectomy and thyroxine (t4) replacement on the spontaneous and human chorionic gonadotropin (hcg)-stimulated secretion of testosterone and the production of adenosine 3',5'-cyclic monophosphate (camp) in rat testes were studied. thyroidectomy decreased the basal levels of plasma luteinizing hormone (lh) and testosterone, which delayed the maximal response of testosterone to gonadotropin-releasing hormone (gnrh) and hcg in male rats. t4 replacement in thyroparathyroidectomiz ...	1999	10733348
serum luteinizing hormone, testosterone, and thyroxine and growth responses of ram lambs fed locoweed (oxytropis sericea) and treated with vitamin e/selenium.	sixteen ram lambs (5 m.o. old, 45 +/- 1.5 kg) received a control diet (50% concentrate, no locoweed, n = 4), locoweed (20% locoweed for 21 d, n = 4), muse (2 ml i.m. of muse containing 5 mg selenium and 50 mg vitamin e/ml, n = 4) on days 21 and 35([day 0 = first day of trial]), or locoweed + muse (n = 4). the rams were maintained in individual pens (3 x 9 m) with free access to feed, water, salt and shade. on day 7 after initiating locoweed, serum alkaline phosphatase (ap) increased (p < 0.01), ...	1999	10735112
nucleic acid synthesis for investigations of gene functions.	the chemical syntheses of genes encoding human c-ha-ras and t4 endonuclease v are described. these genes have been used for studies of mutagenesis by damaged bases and recognition of nucleic acids by proteins. the modes of interaction of photo-damaged dna and cognate antibodies have also been investigated by biochemical and physicochemical approaches.	1999	10780357
direct laser trapping of single globular dna molecules.	we show herein that a single t4 phage dna molecule which was transformed from coiled to globular state, was trapped directly by nd:yag laser (1064 nm). we also estimated trapping force of globular dna molecules.	1999	10780398
in vitro selection of deoxyribozymes with dna capping activity.	in vitro selection was used to isolate a series of deoxyribozymes from a pool of random-sequence dnas that catalyze an atp-dependent self-capping reaction. each deoxyribozyme catalyzes the transfer of the nucleoside and alpha-phosphate moieties of atp to the phosphate group located at its 5' terminus, thereby creating a 5',5'-pyrophosphate cap. this same pyrophosphate cap structure is formed by t4 dna ligase during the classical process of dna ligation. these dna capping enzymes representative o ...	1999	10780467
[total thyroxine (t4), free thyroxine (ft4)].		1999	10778068
conjugated linoleic acid effects on circulating hormones, metabolites and lipoproteins, and its proportion in fasting serum and erythrocyte membranes of swine.	conjugated linoleic acids (cla)# refer to a group of linoleic acid (18:2)-derived isomers with conjugated double bonds mostly at carbon atoms 9 and 11 or 10 and 12, and with all possible cis and trans combinations. cla is a newly recognized nutrient that functions to regulate energy retention and metabolism and that causes a serum lipoprotein profile considered to be less atherogenic. however, rodent models that have been frequently used for these studies are only of limited use because of disti ...	1999	10784383
[risk factors for lymphedema of the arm after mastectomy for breast cancer].	postmastectomy lymphedema of the arm is frequently associated to different factors including axillary node involvement and local and regional treatment of breast cancer. our aims was to identify risk factors of postmastectomy lymphedema. from a retrospective analysis of 735 breast cancers treated in our institute, we found 61 lymphedema of the arm. we then describe our study population and identify by univariate et multivariate analysis the factors significantly associated to the disease. the ma ...	1999	10797983
[lysosomal acid lipase deficiency. overview of czech patients].	lysosomal lipase deficiency is a hereditary autosomal recessive enzymopathy leading to lysosomal storage of triacylglycerols (tag) and cholesterol esters (ce). in particular cells with a permanently high receptor-mediated ldl endocytosis are affected (liver, kidneys). there are two basic phenotypes. the fatal infantile phenotype (wolman's disease) with generalized storage of both types of apolar lipids. this form was diagnosed in this country only once. the opposite is the protracted, oligosympt ...	1999	10746035
hla class i antigens expression in renal cell carcinoma: histopathological and clinical correlation.	immunohistochemical analysis of hla class i antigens expression in 26 renal cell carcinomas (rccs)--18 clear cell, 6 granular and 2 chromophobe--was performed with indirect immunoperoxidase method. results were correlated with extent and immunophenotype of tumor infiltrating mononuclear cells, histopathological (histology, cytology, grade, presence of necrosis) and clinical (tumor diameter, tnm classification) characteristics of rcc. 4 (15%) rccs showed reduced hla class i presence and this was ...	1999	10746977
evidence of interactions between gp27 and gp28 constituents of the central part of bacteriophage t4 baseplate.	the central part of the bacteriophage t4 baseplate consists of several proteins. however, for a number of the constituents the manner of incorporation are not convincingly established. recently, we have presented evidence that gp28 is the structural component of the central part of the baseplate, which possesses a hydrophobic region and is membrane bound [nieradko et al., 1998]. by utilizing extracts prepared from escherichia coli cells that overexpressed genes 27 and 28 of phage t4, we proved t ...	1999	10756710
diagnosis of hypothyroidism in a western lowland gorilla (gorilla gorilla gorilla) using human thyroid-stimulating hormone assay.	primary hypothyroidism was diagnosed in a 26-yr-old female western lowland gorilla (gorilla gorilla gorilla) on the basis of serum levels of thyroxine (t4), free t4, and thyroid-stimulating hormone (tsh) measured by human immunoassays. compared with clinically normal gorillas, the tsh level (107 mliu/l) was markedly elevated, and t4 (<14.0 nmol/l) and free t4 (5.0 pmol/l) levels were decreased. thyroid hypofunction could explain the weight gain, unsettled appetite, anxious behavior, lethargy, an ...	1999	10749441
bacterial colonisation during gtr treatment. a longitudinal analysis.	the purpose of the present study was to evaluate the long-term biological and clinical effects of a controlled delivery system releasing tetracyclines during the healing of interproximal periodontal defects treated by the technique of guided tissue regeneration (gtr) using e-ptfe membranes.	1999	10768009
neonatal screening for hypothyroidism at a university hospital in thailand.	neonatal screening is an essential program for early identification of congenital hypothyroidism. between july 1991 and may 1998, 37, 262 infants born at chulalongkorn hospital were enrolled to the screening program. blood tsh levels were determined on infants at > or = 48 hours after birth. they were performed in dried blood sample taken by heel prick on filter papers. tsh levels were measured by fluoroimmunoassay from july 1991 to december 1997 and by immunoradiometric assay from january to ma ...	1999	11400772
newborn screening for pku and congenital hypothyroidism in latvia.	the newborn screening program in latvia was started in 1980 as pilot study project. mass screening for phenylketonuria (pku) in the whole republic was started in 1987, but for congenital hypothyroidism (ch) it begun in 1996. last two years the latvian state medical genetics center (smgc) screened nearly 25,000 newborn dried blood specimens per year. approximately 25% are repeat and control specimens. efficiency of the screening program is nearly 97%. fluorometric method is used for pku screening ...	1999	11400783
effects of melatonin on the oxidative stress induced by thyrotoxicosis in rats.	high concentrations of thyroid hormones can affect the metabolism of oxygen in aerobic conditions and stimulate free radicals generation in mitochondria. reactive oxygen species are toxic to biomembranes and lead to peroxidation of lipids unless they are not removed by free radical scavengers. a few years ago melatonin (mel), a pineal hormone, was described as a scavenger of free radicals, especially of hydroxyl and peroxyl radicals. the goal of the study was to examine the parameters of oxidati ...	1999	11462107
[relationship between expression of p16 protein and prognosis in carcinoma of nasopharynx].	we observed the relationship between p16 gene and the occurrence, development and prognosis of carcinoma of nasopharynx by means of lsab immunohistochemistry method and investigated the expression of protein p16 in 73 cases of carcinoma of nasopharynx and 10 cases of epithelia of chronic inflammation of nasopharynx. the results showed that p16 positive rate was 100% for the epithelia of chronic inflammation of nasopharynx. it was significantly higher than the p16 positive rate for the carcinoma ...	1999	11387949
taking anti-hiv drugs.	thirteen anti-hiv drugs are currently available by prescription, and most treatment regimens usually consist of a three- or four-drug combination called highly active antiretroviral therapy (haart). haart can be very effective in people who have aids or symptoms of hiv infection. however, for people who feel well and are in the earlier stages of infection, the best time to begin treatment is unclear. side effects of the drugs can be so unpleasant that quality of life is impacted. if the risk of ...	1999	11366273
immune control.	the immune system's job is protection from illness. following a long period of hiv infection, the immune system often fails. opportunistic infections may ensue during this slow process of disease progression. some hiv-positive people, however, do not experience immune system damage, do not develop opportunistic infections, and have undetectable levels of hiv. these long-term nonprogressors are being studied by dr. bruce walker from harvard university. background information on how the immune sys ...	1999	11366299
the structure and action of chitinases.	chitin is second only to cellulose in biomass and it is an important component of many cell wall structures. several families of enzymes, of distinctly different structure, have evolved to hydrolyze this important polysaccaride. glycohydrolase family 18 enzymes, chitinases, are characterized by an eight-fold alpha/beta barrel structure; it has representatives among bacteria, fungi, and higher plants. in general these chitinases act through a retaining mechanism in which beta linked polymer is cl ...	1999	10906956
the evaluation of thyroid and islet autoantibodies in type 1 diabetes mellitus.	type 1 diabetes mellitus is an autoimmune disease in which the presence of different autoantigens can often be found. the aim of our study was to evaluate the prevalence of antibodies against insulin (ia) and autoantibodies against glutamic acid decarboxylase (anti-gad), tyrosine phosphatase ia-2 (anti-ia-2), thyroid microsomal peroxidase (anti-tpo) and thyroglobulin (anti-tg) in 55 randomly selected type 1 diabetic patients (34 males, 21 females). mean age of these patients was 39 +/- 12 yrs, m ...	1999	11221467
gender specificity of resting anterior electroencephalographic asymmetry and defensiveness in the elderly.	it has been reported that defensiveness in women is associated with relative left-frontal electroencephalogram (eeg) activation, while defensiveness in men is associated with relative right-frontal eeg activation. the present study examined whether this result generalized to men and women between the ages of 58 and 70.	1999	11252841
serum thyroid-stimulating-hormone concentration as an index of severity of major depression.	alterations in thyroid axis are common in depression and subclinical hypothyroidism may predispose to recurrent depressive episodes and resistance to antidepressants. the same normal reference ranges are used in both depressive and non-psychiatric patients to detect hypothyroidism. we hypothesized that in depressive patients, serum tsh (thyrotropin) elevation within the normal reference range (>/= upper 25th percentile) may be related to patients' characteristics reflecting the severity of the d ...	1999	11281977
[surgical treatment of urinary bladder cancer].	ultrasonic transabdominal and transrectal investigations, computed tomography, endoscopic photodynamic studies, polyfocal biopsy of the urinary bladder were used in examination of 1238 patients which were diagnosed to have urinary bladder cancer. 894 patients underwent transurethral resection of the bladder. morphologically, cancer of the urinary bladder has an inductory effect on intact parts of the bladder mucosa. this means that even most radical resection does not eliminate grounds for a new ...	1999	11149341
thyroxin and thiouracyl influence upon some biochemical parameters in chicken thymuses.	administration of l-thyroxin in a dose of 30 micrograms/chicken, i.m. and 2-thiouracyl in a dose of 0.3 mg/chicken, i.m. for an interval of 3 days, determined, at the thymus level, increases of total protein content in the tu group, in parallel with decreases of the nitrogen of free amino acids level in the tx group, increases of dna content in the tx group and an increase of the thymus weight in both groups. modifications are due to the action of thyroxin, on the one hand, and on the other to t ...	1999	11068610
thyroid hormones and lipid metabolism in a group of patients over seventy.	numerous studies have suggested a marked correlation between thyroid functionality indices and lipid metabolism. in this trial we assessed the functional parameters of 165 individuals over 70, 87 women and 78 men, correlating the serum values of t3, t4, ft4, tsh with cholesterol, triglycerides, hdl, apo-a and apo-b levels. the correlation was performed over the whole population studied and subsequently, after dividing the population by sex and age (3 age groups: a, 70-75; b, 76-80; c, over 80) i ...	1999	11075619
the initiation of dna base excision repair of dipyrimidine photoproducts.	one of the major dna repair pathways is base excision repair, in which dna bases that have been damaged by endogenous or exogenous agents are removed by the action of a class of enzymes known as dna glycosylases. one subset of the known dna glycosylases has an associated abasic lyase activity that generates a phosphodiester bond scission. the base excision pathway is completed by the sequential action of abasic endonucleases, dna polymerases, and dna ligases. base excision repair of ultraviolet ...	1999	9932454
congenital hypothyroidism screening in oklahoma: a change in follow-up recommendations for 1999.	effective january 1999, the newborn metabolic disorder screening follow-up program of the oklahoma state department of health will no longer track hypothyroidism screen results of one abnormal t4 with normal tsh result. the oklahoma state department of health newborn metabolic disorder screening program (nmdsp) began screening for congenital hypothyroidism (ch) in 1979 and has subsequently identified 159 affected newborns. in 1997, oklahoma had 46,740 live births, 51,431 specimens submitted to n ...	1999	9926672
short-term effects of thyroid hormones on the na/h antiport in l-6 myoblasts: high molecular specificity for 3,3',5-triiodo-l-thyronine.	the thyroid hormones l-t3 and l-t4 were shown to activate the na/h antiport in l-6 cells from rat skeletal muscle by a rapid, nongenomic mechanism. under ph equilibrium conditions, a significant rise in the intracellular ph, measured by the fluorescent ph indicator 2',7'-bis-(carboxyethyl)-5(6)-carboxyfluorescein was observed after the addition of physiological concentrations (10(-10) m) of either l-t3 or l-t4, but with different time courses. l-t3 at all concentrations increased the ph after a ...	1999	9927294
daily melatonin administration at middle age suppresses male rat visceral fat, plasma leptin, and plasma insulin to youthful levels.	human and rat pineal melatonin secretion decline with aging, whereas visceral fat and plasma insulin levels increase. melatonin modulates fat metabolism in some mammalian species, so these aging-associated melatonin, fat and insulin changes could be functionally related. accordingly, we investigated the effects of daily melatonin supplementation to male sprague-dawley rats, starting at middle age (10 months) and continuing into old age (22 months). melatonin was added to the drinking water (92% ...	1999	9927336
dna cleavage and degradation by the sbccd protein complex from escherichia coli.	the sbccd protein is a member of a group of nucleases found in bacteriophage t4 and t5, eubacteria, archaebacteria, yeast, drosophila, mouse and man. evidence from electron microscopy has revealed a distinctive structure consisting of two globular domains linked by a long region of coiled coil, similar to that predicted for the members of the smc family. that a nuclease should have such an unusual structure suggests that its mode of action may be complex. here we show that the protein degrades d ...	1999	9927737
effect of base analog substitutions in the specific gatc site on binding and methylation of oligonucleotide duplexes by the bacteriophage t4 dam dna-[n6-adenine] methyltransferase.	the interaction of the phage t4 dam dna-[n6-adenine] methyltransferase with 24mer synthetic oligonucleotide duplexes having different purine base substitutions in the palindromic recognition sequence, gatc, was investigated by means of gel shift and methyl transfer assays. the substitutions were introduced in either the upper or lower strand: guanine by 7-deazaguanine (g-->d) or 2-aminopurine (g-->n) and target adenine by purine (a-->p) or 2-aminopurine (a-->n). the effects of each base modifica ...	1999	9927748
the high-resolution structure of the triplex formed by the gaa/ttc triplet repeat associated with friedreich's ataxia.	expansions of the triplet repeat, gaa/ttc, inside the first intron of the frataxin gene causes friedreich's ataxia (frda). it was of interest to us to examine whether the frda repeat forms an unusual dna structure, since formation of such structure during replication may cause its expansion. here, we show that the frda repeat forms a triplex in which the ttc strand folds on either side of the same gaa strand. we have determined the high-resolution nmr structures of two intramolecularly folded fr ...	1999	9925783
intra-articular buprenorphine after knee arthroscopy. a randomised, prospective, double-blind study.	demonstration of peripheral opioid receptors in inflamed synovia supports the concept of peripheral opioid analgesia. the aim of this study was to evaluate the analgesic effect of intra-articular administration of buprenorphine after knee arthroscopy.	1999	9926189
the quantitative anatomy of the laminas of the spine.	this study evaluated the dimensions of the laminas from c2 to l5 using adult spine specimens.	1999	9926378
pedicle instrumentation in the thoracic spine. a morphometric and cadaveric study for placement of screws.	in part 1 of the study, the morphometry of thoracic pedicles and bony landmarks for pedicle screw placement were evaluated. in part 2, pedicle screws were inserted in fresh cadavers, using a different entry point in the left and right pedicles.	1999	9926379
endocrine and reproductive function in ewes exposed to the organochlorine pesticides lindane or pentachlorophenol.	the effects of lindane (lin, gamma-hexachlorocyclohexane) and pentachlorophenol (pcp) on reproduction and general endocrine function were examined in breeding ewes as a model for wild and domestic ungulates, which may be exposed to low levels of pesticides that are potential endocrine-disrupting chemicals. ewes (n = 13/group) were fed either a control untreated diet (con), or a diet treated with lin (1 mg/kg/d) or pcp (1 mg/kg/d) during the 5 wk prior to mating and throughout pregnancy and lacta ...	1999	9923752
combination of intrathecal sufentanil 10 mug plus bupivacaine 2.5 mg for labor analgesia: is half the dose enough?	this controlled, double-blinded, prospective trial of 42 parturients in early labor was conducted to determine whether halving the total amount of intrathecal (i.t.) sufentanil and bupivacaine reduced the incidence of systemic hypotension while providing adequate analgesia with minimal lower limb motor block. combined spinal-epidural analgesia (cse) was instituted; group a (n = 21) received a total of 10 microg of sulfentanil plus 2.5 mg of bupivacaine, whereas group b (n = 21) received half tha ...	1999	9972757
thyroid hormones induce features of the hypertrophic phenotype and stimulate correlates of cppd crystal formation in articular chondrocytes.	articular cartilage affected by calcium pyrophosphate dihydrate (cppd) crystal deposition contains abnormal chondrocytes with morphologic similarities to the terminally differentiated hypertrophic chondrocytes that mineralize in growth plate cartilage. these chondrocytes also elaborate high levels of extracellular inorganic pyrophosphate (ppi), an essential component of the cppd crystal. several factors that stimulate articular chondrocyte ppi elaboration also induce terminal differentiation in ...	1999	9972975
bacteriophage t4 gp2 interferes with cell viability and with bacteriophage lambda red recombination.	the t4 head protein, gp2, promotes head-tail joining during phage morphogenesis and is also incorporated into the phage head. it protects the injected dna from degradation by exonuclease v during the subsequent infection. in this study, we show that recombinant gp2, a very basic protein, rapidly kills the cells in which it is expressed. to further illustrate the protectiveness of gp2 for dna termini, we compare the effect of gp2 expression on red-mediated and int-mediated recombination. red-medi ...	1999	9973367
effect of neonatal treatment with msg (monosodium glutamate) on thyroid of the adult male rats.	monosodium glutamate (msg), administered to newborn rats produces extensive lesions in neurons of the arcuate nuclei of the hypothalamus. the cells represent the site of neurohormone production, including the production of both growth-hormone releasing hormone (ghrh) and somatostatin. studies were performed on male wistar strain rats, subcutaneously injected with msg, at 4 mg/g body weight, on days 2, 4, 6, 8 and 10 of life. when the rats reached the age of 18 months, they were additionally stim ...	1999	9987651
concurrent chemotherapy (5-fluorouracil and cisplatin) and radiation therapy followed by surgery for t4 squamous cell carcinoma of the esophagus.	since the prognosis of patients with t4 squamous cell carcinoma (scc) of the esophagus is extremely poor, an effective multimodal treatment needs to be established.	1999	9989417
sacrococcygeal teratoma with an intradural and extramedullary extension in a neonate: case report.	the intradural extension of a sacrococcygeal teratoma is rare and has been reported previously in only two cases. we present a case of a sacrococcygeal teratoma with an intradural and extramedullary extension.	1999	9932895
process engineering strategy for recombinant protein recovery from canola by cation exchange chromatography	the suitability of canola as a recombinant protein production host was evaluated in terms of the potential for simple chromatographic recovery by ion exchange. to investigate the influence of the charge of a recombinant protein on recovery from canola, a series of mutants of t4 lysozyme of varying charge were used to model the situation of transgenic expression by being spiked into nontransgenic canola protein extracts. this mixture was then fractionated by cation exchange chromatography. two di ...	1999	9933509
the in vitro ligation of bacterially expressed proteins using an intein from methanobacterium thermoautotrophicum.	the smallest known intein, found in the ribonucleoside diphosphate reductase gene of methanobacterium thermoautotrophicum (mth rir1 intein), was found to splice poorly in escherichia coli with the naturally occurring proline residue adjacent to the n-terminal cysteine of the intein. splicing proficiency increased when this proline was replaced with an alanine residue. however, constructs that displayed efficient n- and c-terminal cleavage were created by replacing either the c-terminal asparagin ...	1999	9933578
promoting effects of kojic acid due to serum tsh elevation resulting from reduced serum thyroid hormone levels on development of thyroid proliferative lesions in rats initiated with n-bis(2-hydroxypropyl)nitrosamine.	in order to examine whether kojic acid (ka) exerts a promoting effect on thyroid carcinogenesis, male f344 rats were initiated with n-bis(2-hydroxypropyl)nitrosamine (bhp; 2800 mg/kg body wt, single s.c. injection) and, starting 1 week later, received pulverized basal diet containing 2 or 0% ka for 12 weeks. untreated control rats were given basal diet for 13 weeks. as an additional experiment, two groups without bhp initiation received basal diet or diet containing 2% ka for 20 weeks. the serum ...	1999	9934866
uptake of reverse t3 in the human choriocarcinoma cell line, jar.	the uptake and efflux of reverse triiodothyronine (rt3) in jar cells were investigated. uptake of 125i-rt3 was time dependent and reversible with a saturable component of around 70 per cent of total uptake after 30 min of incubation. efflux was not saturable. kinetic analysis of the initial specific uptake rates revealed an uptake process with a michaelis constant of 3.04+/-0.53 microm (mean+/-sem, n=15) and a corresponding maximum velocity of 9.65+/-2.49 pmol/min/mg protein (n=15). uptake of rt ...	1999	9950146
neutralization of tnf does not influence endotoxininduced changes in thyroid hormone metabolism in humans.	to determine the role of tumor necrosis factor (tnf) in endotoxin-induced changes in plasma thyroid hormone and thyroid-stimulating hormone (tsh) concentrations, 24 healthy postabsorptive humans were studied on a control study day (n = 6), after infusion of a recombinant tnf receptor igg fusion protein (tnfr:fc; 6 mg/m2; n = 6) after intravenous injection of endotoxin (2 ng/kg; n = 6), or after administration of endotoxin with tnfr:fc (n = 6). administration of tnfr:fc alone did not affect thyro ...	1999	9950912
treatment of refractory chronic depression and dysthymia with high-dose thyroxine.	an 8-week open trial was conducted to investigate whether patients with treatment-resistant, chronic depression and/or dysthymia could profit from high-dose thyroxine (t4) augmentation.	1999	9951571
acute stress increases thyroid hormone levels in rat brain.	in experimental animals, exposure to uncontrollable stress induces a number of behavioral and biochemical changes that resemble symptoms seen in human depression and other psychiatric conditions. the present study used a yoked design to examine the effects of uncontrollable footshock stress on brain thyroid hormones in male and female rats.	1999	9951572
capnography monitoring during neurosurgery: reliability in relation to various intraoperative positions.	in neurosurgery, estimation of paco2 from petco2 has been questioned. the aim of this study was to reevaluate the accuracy of petco2 in estimating paco2 during neurosurgical procedures lasting >3 h and to measure the effect of surgical positioning on arterial to end-tidal co2 gradient (p[a-et]co2) over time. one hundred four neurosurgical patients classified into four groups (supine [sp], lateral [lt], prone [pr], sitting [st]) were included in a prospective study. paco2, petco2, and p(a-et)co2 ...	1999	9895064
l-atp is recognized by some cellular and viral enzymes: does chance drive enzymic enantioselectivity?	we demonstrate that l-atp is recognized by some enzymes that are involved in the synthesis of nucleotides and nucleic acids. l-atp, as well as its natural d-enantiomer, acts as a phosphate donor in the reaction catalysed by human deoxycytidine kinase, whereas it is not recognized by either enantioselective human thymidine kinase or non-enantioselective herpes virus thymidine kinase. l-atp strongly inhibits (ki 80 microm) the synthesis of rna primers catalysed by dna primase associated with human ...	1999	9895305
total gastrectomy with extended lymphadenectomy for "curable" stomach cancer: experience in a non-japanese asian center.	gastrectomy with extended lymphadenectomy is the advocated treatment in japan for patients with "curable" stomach cancer. attempts in units elsewhere adopting this approach failed to show any survival advantage, and the high operative mortality has prevented global acceptance of the operation. this study examines the safety and efficacy of radical gastrectomy in a far east center outside japan.	1999	9915239
single and multiple selenium-zinc-iodine deficiencies affect rat thyroid metabolism and ultrastructure.	this study was conducted to evaluate the effects of single and combined deficiencies of se, zn and i on thyroid function in rats. rats were fed amino acid-based diets for 6 wk starting from weaning. the diets contained either low or adequate amounts of these minerals. in addition to the control and control pair-fed groups, seven experimental groups were formed: se deficient (se-); i deficient (i-); zn deficient (zn-); se and i deficient (se-i-); zn and i deficient (zn-i-); se and zn deficient (s ...	1999	9915896
novel insight from transgenic mice into thyroid hormone resistance and the regulation of thyrotropin.	patients with resistance to thyroid hormone (rth) exhibit elevated thyroid hormone levels and inappropriate thyrotropin (thyroid-stimulating hormone, or tsh) production. the molecular basis of this disorder resides in the dominant inhibition of endogenous thyroid hormone receptors (trs) by a mutant receptor. to determine the relative contributions of pituitary versus hypothalamic resistance to the dysregulated production of thyroid hormone in these patients, we developed a transgenic mouse model ...	1999	9916139
biochemical interactions within a ternary complex of the bacteriophage t4 recombination proteins uvsy and gp32 bound to single-stranded dna.	the presynaptic phase of homologous recombination requires the formation of a filament of single-stranded dna (ssdna) coated with a recombinase enzyme. in bacteriophage t4, at least three proteins are required for the assembly of this presynaptic filament. in addition to the t4 recombinase, uvsx protein, the t4 ssdna binding protein (gp32), and the uvsy recombination accessory protein are also required. here we report on a detailed analysis of a tripartite filament containing ssdna bound by stoi ...	1999	9893989
biochemical properties of a high fidelity dna ligase from thermus species ak16d.	nad+-dependent dna ligases from thermophilic bacteria thermus species are highly homologous with amino acid sequence identities ranging from 85 to 98%. thermus species ak16d ligase, the most divergent of the seven thermus isolates collected worldwide, was cloned, expressed in escherichia coli and purified to homogeneity. this thermus ligase is similar to thermus thermophilus hb8 ligase with respect to ph, salt, nad+, divalent cation profiles and steady-state kinetics.however, the former is more ...	1999	9889274
high sequence fidelity in a non-enzymatic dna autoligation reaction.	the success of oligonucleotide ligation assays in probing specific sequences of dna arises in large part from high enzymatic selectivity against base mismatches at the ligation junction. we describe here a study of the effect of mismatches on a new non-enzymatic, reagent-free method for ligation of oligonucleotides. in this approach, two oligonucleotides bound at adjacent sites on a complementary strand undergo autoligation by displacement of a 5'-end iodide with a 3'-phosphorothioate group. the ...	1999	9889286
effect of thyroid status on lipid composition and peroxidation in the mouse liver.	in order to analyze the possible relationship between metabolic rate and oxidative stress, of1 female mice were rendered hyper- or hypothyroid for 4-5 weeks by administration of 0.0012% l-thyroxine (t4) or 0.05% 6-n-propyl-2-thiouracil (ptu), respectively, in their drinking water. treatment with t4 resulted in increased basal metabolic rate measured by oxygen consumption and liver cytochrome oxidase activity without altering the glutathione redox system. endogenous lipid peroxidation, sensitivit ...	1999	9890642
replication block by an enediyne drug-dna deoxyribose adduct.	under anaerobic conditions neocarzinostatin chromophore, an enediyne antibiotic, forms a covalent drug-dna adduct on the 5' carbon of deoxyribose at a specific single site in a 2-nucleotide bulge, rather than strand cleavage, by a mechanism involving general base-catalyzed intramolecular drug activation to a reactive radical species. we have taken advantage of the selectivity of this reaction to prepare a single-stranded oligonucleotide containing a single drug adduct at a t residue and to study ...	1999	9890903
video-assisted thoracoscopic surgery to the upper thoracic spine.	the standard open technique for exposure of the upper thoracic spine, t1-t4, usually requires a difficult thoracotomy. from november 1, 1995 to june 30, 1997, eight patients underwent video-assisted thoracoscopic spinal surgery in our institute to treat their upper thoracic spinal lesions endoscopically.	1999	9918611
the bacteriophage t4 transcriptional activator mota accepts various base-pair changes within its binding sequence.	during infection, bacteriophage t4 regulates three sets of genes: early, middle, and late. the host rna polymerase is capable of transcribing early genes, but middle transcription requires the t4-encoded transcriptional activator, mota protein, and the t4 co-activator, asia protein, both of which bind to the sigma 70 (sigma70) subunit of rna polymerase. mota also binds a dna sequence (a mota box), centered at position -30. the identification of more than 20 middle promoters suggested that a stro ...	1999	9918715
association of holliday-structure resolving endonuclease vii with gp20 from the packaging machine of phage t4.	endonuclease vii (endo vii) is the product of gene 49 (gp49) of bacteriophage t4. it is a holliday-structure resolvase (x-solvase) responsible for clearing branched replicative dna prior to packaging. consequently, mutations in gene 49 are unable to fill heads to completion because unresolved branches stop translocation of dna. a likely association of gp49 with heads or proheads, however, could not be shown in the past. we have investigated whether gp49 could be part of the transiently assembled ...	1999	9918721
identification of thyroid hormone transporters.	thyroid hormone action and metabolism are intracellular events that require transport of the hormone across the plasma membrane. we tested the possible involvement of the na+/taurocholate cotransporting polypeptide (ntcp) and organic anion transporting polypeptide (oatp1) in the hepatic uptake of the prohormone t4, the active hormone t3, and the metabolites rt3 and 3,3'-t2. xenopus laevis oocytes were injected with 2.3 ng ntcp or oatp1 crna and, after 2-3 days, incubated for 1 h at 25 degrees c ...	1999	9918867
induction chemotherapy for t4 centrally located non-small cell lung cancer.	we used induction chemotherapy in a prospective, single-institution clinical trial intended to achieve resectability in patients with centrally located, unresectable t4 non-small cell lung cancer. other types of iiib disease were excluded.	1999	9918961
clinical and biochemical phenotype of familial anterior hypopituitarism from mutation of the prop1 gene.	we have investigated the largest family with prop1 deficiency reported to date. eight patients, aged 17-40 yr, in two sibships with possibly related mothers but no parental consanguinity were 109-137 cm in height (-8.8 to [minus]5.9 sd score) and sexually immature. none had received hormonal therapy. affected individuals had similarities to and significant differences from patients with insulin-like growth factor i (igf-i) deficiency due to gh receptor deficiency (ghrd) and normal thyroid functi ...	1999	9920061
characterisation of cholera toxin by liquid chromatography--electrospray mass spectrometry.	cholera toxin, one of the toxins that may be generated by various strains of the bacterium vibrio cholerae, can be considered as a substance possibly used in biological warfare. the possibilities of characterising the toxin by liquid chromatography electrospray mass spectrometry (lc-es-ms) were investigated. the toxin can be detected by flow-injection (fia) es-ms of a dialysed solution and observation of the charge envelope signals of its a-unit and b-chain protein; sufficient information for id ...	1999	9920483
significance of interictal temporal lobe delta activity for localization of the primary epileptogenic region.	because interictal temporal lobe delta activity (tlda) has been described in 30 to 90% of patients with temporal lobe epilepsy (tle) but has not been investigated in patients with extratemporal epilepsy, we sought to determine the localizing significance of tlda. we compared the presurgical interictal scalp eeg results of 47 consecutive patients who received extratemporal resection (40 frontal and 7 parietal-occipital) for intractable epilepsy with 43 consecutive patients who received anterior t ...	1999	9921879
management of the n0 neck during salvage laryngectomy.	radiotherapy is effective treatment for laryngeal carcinoma. early-stage laryngeal carcinoma has a low incidence of cervical metastasis. patients initially clinically n0 usually remain n0 when they fail at the primary site. the incidence of subclinical metastasis in these patients is not well described. watchful waiting or elective neck dissections are advocated.	1999	9917031
a correlation between the loss of hydrophobic core packing interactions and protein stability.	the hydrophobic core packing in four-alpha-helical bundles appears to be crucial for stabilizing the protein structure. to examine the structural basis of hydrophobic stabilization, the crystal structures of the leu-->val (l41v) and leu-->ala (l41a) substitutions of the core residue leu41 of the rop protein have been determined. both substitutions are destabilizing and lead to formation of cavities. the main responses to mutations are the collapse of the central part of the alpha-helix containin ...	1999	9878446
long days and thyroxine program american tree sparrows for seasonality: evidence for temporal flexibility of the breeding season of euthyroid females.	to explore the role of the thyroid in the control of seasonality, photosensitive female american tree sparrows (spizella arborea) were thyroidectomized (thx), moved to long days, and given daily injections of thyroxine (t4) for 3 weeks; thx and thyroid-intact (thi) controls received daily injections of alkaline vehicle. birds were retained on long days 4 additional weeks and then moved to constant light and given t4 in drinking water for 5 weeks in order to test for photorefractoriness. endpoint ...	1999	9882552
prostate-specific antigen to predict outcome of external beam radiation for prostate cancer: walter reed army medical center experience, 1988-1995.	to assess the ability of pretreatment and post-treatment prostate-specific antigen (psa) measurements, clinical tumor stage, tumor grade, gleason sum, race, age, and radiation dose to predict the recurrence of prostate cancer following external beam radiation therapy (xrt) since the introduction of psa as a tumor marker at one tertiary care center.	1999	9886602
feedback regulation of thyrotropin-releasing hormone gene expression by thyroid hormone in the caudal raphe nuclei in rats.	medullary trh regulates autonomic activity, and altered thyroid status is associated with autonomic disorders. we investigated whether thyroid hormone exerts a negative feedback regulation on trh gene expression in the medullary caudal raphe nuclei. medullary pro-trh messenger rnas (mrnas) were mainly located in the raphe pallidus and raphe obscurus neurons as shown by in situ hybridization and were significantly increased by 70% and 160-230% by northern blot analyses in 24 h fasted rats at 1 an ...	1999	9886805
compensatory changes in groel/gp31 affinity as a mechanism for allele-specific genetic interaction.	previous work has shown that the groel-groes interaction is primarily mediated by the groes mobile loop. in bacteriophage t4 infection, groes is substituted by the gene 31-encoded cochaperonin, gp31. using a genetic selection scheme, we have identified a new set of mutations in gene 31 that affect interaction with groel; all mutations result in changes in the mobile loop of gp31. biochemical analyses reveal that the mobile loop mutations alter the affinity between gp31 and groel, most likely by ...	1999	9867810
lysis and lysis inhibition in bacteriophage t4: rv mutations reside in the holin t gene.	upon infecting populations of susceptible host cells, t-even bacteriophages maximize their yield by switching from lysis at about 25 to 35 min at 37 degrees c after infection by a single phage particle to long-delayed lysis (lysis inhibition) under conditions of sequential infection occurring when free phages outnumber host cells. the timing of lysis depends upon gene t and upon one or more rapid-lysis (r) genes whose inactivation prevents lysis inhibition. t encodes a holin that mediates the mo ...	1999	10400598
molecular heterogeneity of oligodendrocytes in chicken white matter.	the classical studies by del rio hortega (mem. real. soc. espan. hist. nat. 14:40-122, 1928) suggest that the oligodendrocyte population includes four morphological subtypes. recent data from the cat and the rat show that the anatomy of oligodendrocytes related to early myelinating prospective large fibers differs from that of oligodendrocytes related to late myelinating prospective small fibers. after application of a polyclonal antiserum to cryostat sections from the chicken cns, we noted that ...	1999	10401628
treatment of the clinically negative neck in oral squamous cell carcinoma.	to define the most effect treatment plan of patients with oral cavity squamous cell carcinoma with clinically negative (no) neck staging.	1999	10401861
squamous cell carcinoma of the nasal vestibule.	sixty patients were treated with radiation therapy alone (56 patients) or followed by surgery (4 patients) between 1970 and 1995 for squamous cell carcinoma of the nasal vestibule.	1999	10402517
tissue-specific patterns of changes in 3,5,3'-triiodo-l-thyronine concentrations in thyroidectomized rats infused with increasing doses of the hormone. which are the regulatory mechanisms?	we have measured 3,5,3'triiodothyronine (t3) in 12 tissues from thyroidectomized (tx) rats infused with increasing doses of t3, and related them to their corresponding plasma levels. young adult wistar rats were surgically tx. after 4 weeks, the animals were infused with placebo or t3 (0.25, 0.50, 0.75, 1.00 or 2.00 microg/100 g body weight/day). placebo-infused intact rats served as euthyroid controls. plasma and samples of cerebral cortex, cerebellum, brown adipose tissue (bat), pituitary, liv ...	1999	10403175
the importance of thyroglobulin structure for thyroid hormone biosynthesis.	thyroglobulin (tg) is the most important protein in the thyroid because it provides the matrix for thyroid hormone biosynthesis. here we review experimental work, principally from our laboratory, on the relationship between tg structure and hormonogenesis. early work showed that tg's most important hormonogenic site was located in a fragment of approximately 26 kda obtained on chemical reduction. with the establishment of the cdna sequence of tg, this and other major sites could be localized wit ...	1999	10403182
mutation of the secys residue 266 in human type 2 selenodeiodinase alters 75se incorporation without affecting its biochemical properties.	type 2 deiodinase (d2) is a low km iodothyronine deiodinase that metabolizes thyroxine (t4) to the active metabolite t3. we have recently shown that the cdna for the human d2 coding region contains two in-frame selenocysteine (tga) codons. the 3' tga is seven codons 5' to a universal stop codon, taa. the human d2 enzyme, transiently expressed in hek-293 cells, can be in vivo labeled with 75se as a doublet of approximately 31 kda. this doublet is consistent with the possibility that the carboxy-t ...	1999	10403186
altered thyroid hormone binding to plasma lipoproteins in the syndrome of resistance to thyroid hormones.	lipoproteins, especially hdl, are carriers of a small fraction of the thyroid hormones in plasma and participate in the intracellular transport of t4. in previous work we showed that a brief period of hypothyroidism alters the hormone distribution among the lipoproteins, causing a decrease in vldl and ldl binding despite a relative increase in vldl and ldl cholesterol, an increase in hdl binding, and a reversal of t4 and t3 binding to the smallest hdl size subgroup. the present study of three pa ...	1999	10403188
hormone synthesis and storage in the thyroid of human preterm and term newborns: effect of thyroxine treatment.	iodine and thyroglobulin concentrations, as well as iodine, t3, t4 and sialic acid contents of thyroglobulin, were measured in thyroid glands collected postmortem from 42 human premature or term newborns and infants. three groups were considered: very preterm newborns (24-32 postmenstrual weeks, < 5 days postnatal life), preterm and term newborns (34-41 postmenstrual weeks, < 5 days postnatal life) and infants (born at term, postnatal age 1-8 months). five very preterm and seven preterm newborns ...	1999	10403191
dietary fat and protein interactions in the broiler.	an experiment was conducted to study the interrelationships between dietary fat and protein levels in the regulation of lipid metabolism in the broiler chicken. birds were fed diets containing 300, 600, or 1,200 kcal me from fat (corn oil) with either 124 or 190 g cp/kg. two additional experimental diets contained 234 or 285 g cp and 300 kcal me from fat. regardless of fat level, birds fed the diets containing 124 g cp/kg weighed less and were less efficient than birds fed diets containing 190 g ...	1999	10404679
the development of graves' disease and the ctla-4 gene on chromosome 2q33.	case-control studies suggest that the ctla-4 gene may be a susceptibility locus for graves' disease. the previously reported a/g polymorphism at position 49 in exon 1 of the ctla-4 gene was, therefore, investigated in a case-control (n = 743) and family-based (n = 179) dataset of white caucasian subjects with graves' disease. the relationship between ctla-4 genotype and severity of thyroid dysfunction at diagnosis was also investigated. an increase in frequency of the g (alanine) allele was seen ...	1999	10404810