Publications

[hyperthyroidism induced by iodinated roentgen contrast media].	three patients with subclinical hyperthyroid goitre, women aged 63, 72 and 75 years following intravenous administration of an iodinated contrast medium developed hyperthyroidism with a marked rise of the concentration of free t4. thyreostatic agents were unsuccessful in two patients, the third was left untreated. hyperthyroidism improved spontaneously in all three. iodine-induced hyperthyroidism is rare and is usually encountered in patients with a pre-existent autonomous thyroid function. trea ...	1998	9763853
perioperative cytokine release during coronary artery bypass grafting in patients of different ages.	surgical interventions and cardiopulmonary bypass (cpb) induce a systemic inflammatory response with cytokine release. ageing is perceived as a process of impaired immune functions: il-1beta, il-6 and tumour necrosis factor-alpha (tnf-alpha) secretion are increased while il-2 release is reduced in advanced age. at present, little information is available about perioperative immune reactions at different stages of ageing. the aim of the present study was to compare il-6, il-1beta, tnf-alpha, il-1 ...	1998	9764599
cloning and expression of a novel, tissue specifically expressed member of the udp-galnac:polypeptide n-acetylgalactosaminyltransferase family.	we report the cloning and expression of the fifth member of the mammalian udp-galnac:polypeptide n-acetylgalactosaminyltransferase (ppgantase) family. degenerate polymerase chain reaction amplification and hybridization screening of a rat sublingual gland (rslg) cdna library were used to identify a novel isoform termed ppgantase-t5. conceptual translation of the cdna reveals a uniquely long stem region not observed for other members of this enzyme family. recombinant proteins expressed transient ...	1998	9765313
changes in thyroid hormone levels during growth hormone therapy in initially euthyroid patients: lack of need for thyroxine supplementation.	the occurrence of central hypothyroidism in previously euthyroid children during gh therapy has been reported with widely varying incidence. we monitored the acute effects on the hypothalamic-pituitary-thyroid axis in 15 euthyroid children with classic gh deficiency during the first year of gh therapy. all were initially euthyroid, as assessed by normal baseline tsh, t4, free t4, and t3 levels and negative antithyroid antibodies. a thyroid profile (t4, free t4 index, t3, rt3, and tsh) was perfor ...	1998	9768652
circulating iodide concentrations during and after pregnancy.	early, indirect studies suggested that an important aspect of thyroid economy during pregnancy was a decline in plasma or serum inorganic iodide (pii) concentrations, but there is little information concerning circulating iodide concentrations as assessed by direct measurement. the present study was undertaken to determine the relationship between gestation and serum iodide concentrations as assessed by direct measurement of pii. pii concentrations, urinary iodide levels, and other parameters of ...	1998	9768662
complete thyroxine-binding globulin (tbg) deficiency produced by a mutation in acceptor splice site causing frameshift and early termination of translation (tbg-kankakee).	fourteen t4-binding globulin (tbg) variants have been identified at the gene level. they are all located in the coding region of the gene and 6 produce complete deficiency of tbg (tbg-cd). we now describe the first mutation in a noncoding region producing tbg-cd. the proband was treated for over 20 yr with l-t4 because of fatigue associated with a low concentration of serum total t4. fifteen family members were studied showing low total t4 inherited as an x chromosome-linked trait, and affected ...	1998	9768672
low growth hormone-binding protein in infants with congenital hypothyroidism.	we evaluated the circulating levels of gh, insulin-like growth factor i (igf-i), gh-binding protein (ghbp), and igf-binding protein-3 (igfbp-3) before l-t4 therapy in 19 infants with congenital hypothyroidism (ch), aged 12-29 days, diagnosed by neonatal screening and in a group of age- and sex-matched control infants. the same parameters were reevaluated after several months of treatment. serum ghbp was measured by the high performance liquid chromatography-gel filtration method; serum gh, igf-i ...	1998	9768678
the advantages of the lateral decubitus position after spinal anesthesia with hyperbaric tetracaine.	we investigated the effects of lateral decubitus positioning after spinal anesthesia with hyperbaric tetracaine on the spread of sensory blockade and hemodynamic variables. one hundred asa physical status i or ii patients scheduled for elective surgery to the lower limb received spinal anesthesia at a rate of approximately 0.1 ml/s using 0.5% tetracaine in 7.5% glucose with 0.125% phenylephrine in the lateral decubitus position with the operated side dependent. they were randomly divided into th ...	1998	9768787
concentrations of vascular endothelial growth factor in the sera of gastric cancer patients.	vegf (vascular endothelial growth factor) is known to play crucial roles in tumor angiogenesis. in 281 gastric cancer patients, aberrant increase of vegf level was observed in 36 patients (12.8%). in 14 recurrent patients, 8 showed an increase of vegf. the serum vegf levels of stage iv cancer were significantly higher than those of stage i. the serum levels of recurrent patients were significantly higher than those of stage i, ii and iii. vegf levels of patients with serosal invasion were signif ...	1998	9769380
[cellular and humoral immunity in atopic dermatitis].	in vitro tests of cellular and humoral immunity were analysed by: t and b lymphocytes, t4 and t8 lymphocyte subsets, lymphocyte blast transformation tests with unspecified mitogenom--phytohemaglutinin, c3 and c4 components of the complement and circulating immune complexes at patients from 6 to 54 years old who suffered from atopic dermatitis without any other clinical symptom of atopy (so called "pure" atopic dermatitis). parameters of cellular and humoral immunity were analysed in the active a ...	1998	9769640
efficacy of dose-intensified mec (methotrexate, epirubicin and cisplatin) chemotherapy for advanced urothelial carcinoma: a prospective randomized trial comparing mec and m-vac (methotrexate, vinblastine, doxorubicin and cisplatin). japanese urothelial cancer research group.	to evaluate the antitumor activity in patients with t3b, t4 or metastatic urothelial carcinoma treated with mec or m-vac chemotherapy, by performing a multi-center randomized prospective study.	1998	9769784
characterisation of the bacteriophage t4 comc alpha 55.6 and comcj mutants. a possible role in an antitermination process.	we have performed a new screen for t4 mutants (comc) that overcome the phage growth restriction caused by the escherichia coli rho/tabc mutants. we show that one such mutant (comcj) identifies a different gene from that identified by canonical comc mutants. we compare the regulation of t4 prereplicative transcription in a rho/tabc mutant infected by t4 wild-type, by a canonical comc mutant (comc alpha 55.6) and by comcj. the transcription rates of the two prereplicative genes 39 and 43 is depres ...	1998	9770273
deficient dna-ligase activity in the metabolic disease tyrosinemia type i.	hereditary tyrosinemia type i (ht1) is an autosomal recessive inborn error of metabolism caused by the deficiency of fumarylacetoacetate hydrolase, the last enzyme in the tyrosine catabolism pathway. this defect results in accumulation of succinylacetone (sa) that reacts with amino acids and proteins to form stable adducts via schiff base formation, lysine being the most reactive amino acid. ht1 patients surviving beyond infancy are at considerable risk for the development of hepatocellular carc ...	1998	9770534
the influence of intrathecal fentanyl on the characteristics of subarachnoid block for caesarean section.	forty healthy parturients scheduled for elective caesarean section were randomly allocated to receive either 0.3 ml 0.9% saline (control group, n = 20), or 15 micrograms (0.3 ml) fentanyl (treatment group, n = 20) added to 2.5 ml 0.5% hyperbaric bupivacaine given intrathecally in the sitting position. a sensory block to t4 was achieved after 6.5 min in those who received fentanyl compared to 8.0 min in the control group; this was not significantly different. the highest level of sensory block ac ...	1998	9771182
synthesis and biochemical study of n2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-(alpha,beta-imido)triphosphate (bupdgmpnhpp): a non-substrate inhibitor of b family dna polymerases.	bupdgmpnhpp was synthesized and assayed as a non-incorporable inhibitor of b family dna polymerases. the derivative was synthesized by preparation of the imidophosphorane of bupdg followed by reaction with orthophosphate using the imidazolide method. bupdgmpnhpp inhibited human dna polymerase alpha and t4 dna polymerase 10 and 3.5-times more potently than bupdgtp, respectively, and was not a substrate for either enzyme. bupdgmpnhpp acts as an active site affinity probe that could find use in co- ...	1998	9672707
clinical comparison and performance perspective of three aligning arch wires.	to clinically evaluate three commonly used orthodontic tooth aligning arch wires: 016 x 022 inch active martensitic medium force nickel titanium, 016 x 022 inch graded force active martensitic nickel titanium, and 0.0155 inch multistrand stainless steel.	1998	9674677
thyroid profile modifications during oral hormone replacement therapy in postmenopausal women.	there are few data available about changes in thyroid hormone profiles after hormone replacement therapy (hrt). we analyzed the effect of two different oral estrogens/progestins (e/p) associations on thyroid hormones and thyroxine-binding globulin (tbg) levels in 14 postmenopausal normal women distributed at random into two groups. both groups received daily for a year 2 mg of estradiol valeriante per os. in group a (n = 7), estrogen was associated with norethisterone acetate. in group b, estrog ...	1998	9675564
how to diagnose hypopituitarism. learning the features of secondary hormonal deficiencies.	hypopituitarism has many causes and various clinical presentations. diagnosis depends on history taking, clinical suspicion, and an understanding of the hypothalamic-pituitary-target organ axes for proper interpretation of laboratory data. in the patient described in the case report, amenorrhea and inability to lactate were early clinical clues to a possible pituitary problem, but she felt otherwise fairly well over the years and did not seek evaluation. thyroid-function tests showed normal thyr ...	1998	9676563
telomerase activity in oral squamous cell carcinoma.	the riboprotein telomerase has been linked to cellular immortality and is believed to play a key role in tumorigenesis.	1998	9677114
a comparative study of the tryptase release test and the cellular allergen stimulation test (cast) in mite sensitive patients.	the stimulation of blood basophils to release mediators in vitro is widely used for diagnosis of allergic diseases. tryptase release and sulphidopeptideleukotriene production are both triggered by cross-bridging of adjacent ige molecules on the surface of ige-bearing basophils.	1998	9677141
a general rule for the relationship between hydrophobic effect and conformational stability of a protein: stability and structure of a series of hydrophobic mutants of human lysozyme.	to get a general rule for the relationship between hydrophobic effect and conformational stability, five ile to val and nine val to ala mutants (3ss mutants) from 3ss (c77a/c95a) human lysozyme were constructed. as known from previous studies, the 3ss protein lacking a disulfide bond between cys77 and cys95 is destabilized by enthalpic factors, as revealed by a decrease of about 20 kj/mol in the denaturation gibbs energy change (deltag) value, as compared to the wild-type protein, which has four ...	1998	9677301
lymphocyte subsets in superficial bladder cancer: correlation with biological activity of the tumours.	the investigation of relationship of tumour biological activity and the degree of tissue infiltrating lymphocytes (t4, t8, t6) and macrophages in bladder cancer was the purpose of this study.	1998	9677463
[paranasal sinus carcinomas with orbital involvement].	fifteen patients at the university of mainz were diagnosed as having t3 or t4 carcinomas of the paranasal sinuses that invaded the orbit. treatment involved surgical resections of tumor with adjacent periorbita but without enucleation in an attempt to preserve vision and/or cosmesis. survival rates and especially the rate, time and locations of recurrences were investigated. analysis of the data revealed that preservation of the eye in these cases with advanced sinus carcinomas with orbital tumo ...	1998	9677492
analytical performance of elecsys 2010--a multicentre evaluation.	the analytical performance of elecsys 2010 has been assessed in a multicentre evaluation, which involved twelve laboratories from eight countries worldwide. eleven alanytes (tsh, t4, ft4, t3, ft3, t-up, tnt, ck-mb, hcg, cea and psa) were tested using a protocol related to the eccls guidelines and the standard operating procedures of the manufacturer. the evaluation was supported by a "computer aided evaluation" (caev) program system and telecommunications. within-run and between-day imprecision ...	1998	9677668
elecsys tsh, ft4, t4, t-uptake, ft3 and t3. clinical results of a multicentre study.	6 assays for the assessment of thyroid function (tsh, ft4, t4, t-uptake, ft3 and t3) were targets of the international multicenter study on the random access analyzer elecsys 2010. the aim of the study was to characterize the clinical performance of the assay in method comparison and reference range studies. the assays under evaluation were compared to a broad variety of radio isotopic and non-radio isotopic assays. they are suitable for serum and plasma samples. in case of tsh the study include ...	1998	9677670
markers of bone turnover in patients with differentiated thyroid cancer with and following withdrawal of thyroxine suppressive therapy.	to study whether levothyroxine (lt4) suppressive therapy exposes patients with differentiated thyroid cancer (tc) to an increased risk of osteoporosis.	1998	9678534
influence of dietary habits on thyroid status of a nomadic people, the bororo shepherds, roaming a central african region affected by severe iodine deficiency.	in contrast with the endemic goiter reported in several african countries, the nomadic bororos of the central african republic have an unexpectedly low prevalence of goiter. this study was conducted to elucidate this puzzling observation.	1998	9678536
effects of marginal iodine deficiency on thyroid hormone production, distribution and transport in nonpregnant and near-term pregnant rats.	during pregnancy maternal thyroid hormones are of great importance for normal development of the central nervous system of the fetus. iodine deficiency of the mother carl result in an impaired development of the fetal brain. in large areas of the world iodine intake is moderately low. to study the effects of marginal iodine deficiency (mid) on the production, distribution, and transport of thyroxine (t4) and 3,5,3'-tri-iodothyronine (t3) in nonpregnant and near-term pregnant rats we performed ki ...	1998	9678542
dehydroepiandrosterone sulfate does not prevent spontaneous and iodine-induced lymphocytic thyroiditis and diabetes mellitus in the bb/wor rat.	chronic feeding of dehydroepiandrosterone (dhea) and its sulfated metabolite, dehydroepiandrosterone sulfate (dheas), has previously been reported to decrease hyperglycemia, obesity, cancer, and autoantibody generation in a number of animal models and to increase muscle mass and physiological and psychological well-being in elderly humans, although these latter studies remain controversial. the present study was carried out to determine whether large amounts of dheas given orally would prevent t ...	1998	9678543
3,5,3'-triiodothyronine (t3) clearance and t3-glucuronide (t3g) appearance kinetics in plasma of freshwater-reared male tilapia, oreochromis mossambicus.	distribution and metabolism of the thyroid hormone 3,5, 3'-l-triiodothyronine (t3) were studied in several ways to gain insights into these processes in the warm water fish tilapia oreochromis mossambicus. trace doses of 125i-labeled t3 (t*3)1 were injected intraarterially, extraarterially, or intraperitoneally in freshwater-reared male tilapia to explore plasma clearance kinetic responses to these different input modalities. multicompartmental analysis of the plasma clearance data indicated a k ...	1998	9679085
metamorphosis in the summer flounder (paralichthys dentatus): stage-specific developmental response to altered thyroid status.	the summer flounder (paralichthys dentatus) undergoes a true metamorphosis during which the bilaterally symmetrical larva transforms into an asymmetrical juvenile. this study addresses the influence of manipulating thyroid status on larval development and growth. development was assessed by categorizing larvae in stages based on the position of the translocating eye and growth was assessed as a change in total length. larvae were raised in seawater or in seawater containing thyroxine-sodium salt ...	1998	9679087
pituitary and extrapituitary action sites of the novel nonpeptidyl growth hormone (gh) secretagogue l-692,429 in the chicken.	chickens were used as a model to further analyze the efficacy and specificity of l-692,429, a novel nonpeptidyl mimic of growth hormone (gh)-releasing peptide-6 (ghrp-6), which is a specific gh-releasing secretagogue in mammals. actions at the level of the pituitary and the hypothalamus were studied. pituitaries isolated from 1-day-old (c1) chicks responded in a dose-dependent manner to l-692,429 (ed50 = 10 nm). using equimolar concentrations of thyrotropin-releasing hormone (trh), human gh-rele ...	1998	9679090
influences of thyroid stimulating hormone on cognitive functioning in very old age.	this study investigated the relationship of thyroxine (t4) and thyroid stimulating hormone (tsh) within normal ranges to cognitive performance in very old age. the participants (n = 200) were selected from a population-based study of nondemented persons aged 75 to 96 years (m = 83.9 years). tasks assessing episodic memory, verbal fluency, visuospatial ability, short-term memory, and perceptual-motor speed were examined. results indicated that t4 was unrelated to performance. however, tsh was pos ...	1998	9679515
engineering trimeric fibrous proteins based on bacteriophage t4 adhesins.	the adsorption specificity of bacteriophage t4 is determined by genes 12 and 37, encoding the short tail-fibers (stf) and the distal part of the long tail-fibers (ltf), respectively. both are trimeric proteins with rod domains made up of similar tandem quasi-repeats, approximately 40 amino acids long. their assembly requires the viral chaperones gp57a and gp38. here we report that fusing fragments of gp12 and gp37 to another trimeric t4 fibrous protein, fibritin, facilitates correct assembly, th ...	1998	9680195
rat brain type ii 5'-iodothyronine deiodinase activity is extremely sensitive to stress.	the effects of different kinds of acute stressor on thyroid hormone concentrations and deiodinase activities were investigated in four brain regions (frontal cortex, amygdala, hypothalamus, and cerebellum) and in the pituitaries and livers of adult male rats. five groups of rats were killed after each of the following stressors: (a) an intraperitoneal injection of saline, (b) intragastric intubation, (c) and (d) two different forms of handling, being grasped as for intraperitoneal injection and ...	1998	9681474
recent developments in the investigation of thyroid regulation and thyroid carcinogenesis.	this review covers new mechanistic information spanning the past 10 years relevant to normal and abnormal thyroid growth and function that may assist in the risk assessment of chemicals inducing thyroid follicular cell neoplasia. recent studies have shown that thyroid regulation occurs via a complex interactive network mediated through several different messenger systems. increased thyroid-stimulating hormone (tsh) levels activate the signal transduction pathways to stimulate growth and differen ...	1998	9681969
cell surface expression of 70 kda heat shock protein in human oral dysplasia and squamous cell carcinoma: correlation with clinicopathological features.	to understand the role of 70 kda heat shock protein (hsp70) in the pathogenesis of oral cancer, its expression was studied in human normal, premalignant and malignant oral mucosa. cell surface expression of hsp70 was assessed in oral tissue specimens by flow cytometric analysis using a mouse monoclonal antibody against hsp70. a significant increase in cell surface expression of hsp70 was observed as the tissue progressed from normal to dysplasia towards carcinoma. correlation of hsp70 expression ...	1998	9682770
ambulation by traumatic t4-12 paraplegics using functional neuromuscular stimulation	the review describes and discusses the status of the major approaches to functional neuromuscular stimulation (fns) for the purpose of ambulation by traumatic complete thoracic-level paraplegics, namely, hybrid body bracing with fns, percutaneous fns, fully implanted fns, and transcutaneous fns. for each approach, the review discusses the system for which the most published user data are available and, where relevant, where the largest user population exists. the review covers methodologies and ...	1998	9683682
triple helix formation at (at)n adjacent to an oligopurine tract.	we have used dnase i footprinting to investigate the recognition of (at) n tracts in duplex dna using gt-containing oligonucleotides designed to form alternating g.ta and t.at triplets. previous studies have shown that the formation of these complexes is facilitated by anchoring the triplex with a block of adjacent t.at triplets, i.e. using t11(tg)6to recognize the target a11(at)6. (at)6t11. in the present study we have examined how the stability of these complexes is affected by the length of e ...	1998	9685475
interfemur variation of bone mineral density in patients receiving high-dose thyroxin therapy.	in order to evaluate the interfemoral variability of bone mineral density (bmd) in patients receiving thyroxin (t4) replacement therapy, dual-energy x-ray absorptiometry (dxa) was performed on both hips and the lumbar spine of 114 individuals. bmd was measured in 47 patients under t4 therapy in suppressive doses because of histologically proven thyroid cancer and 67 age-matched controls free of any known local or generalized disorder that would affect the bones and joints. variation in bmd betwe ...	1998	9685511
autoradiographic thyroid evaluation in short-term experimental diabetes mellitus.	previous studies have shown that in vitro thyroid peroxidase (tpo) iodide oxidation activity is decreased and thyroid t4-5'-deiodinase activity is increased 15 days after induction of experimental diabetes mellitus (dm). in the present study we used thyroid histoautoradiography, an indirect assay of in vivo tpo activity, to determine the possible parallelism between the in vitro and in vivo changes induced by experimental dm. dm was induced in male wistar rats (about 250 g body weight) by a sing ...	1998	9686152
[hypothyroidism--clinical findings, diagnosis, therapy. thyroid tests should be performed on broad indications].	hypothyroidism is a clinical entity resulting from deficiency of thyroid hormones or, more rarely, from their impaired activity at tissue level. it is a common condition, with a prevalence of 1.9 per cent in women, and the prevalence increases with age. hypothyroidism may be congenital or acquired, primary or secondary, chronic or transient. primary hypothyroidism is caused by disease or treatment which destroys the thyroid gland or interferes with thyroid hormone biosynthesis. autoimmune thyroi ...	1998	9772803
vasopressor hormone response following mesenteric traction during major abdominal surgery.	we investigated the vasopressor hormone response following mesenteric traction (mt) with hypotension due to prostacyclin (pgi2) release in patients undergoing abdominal surgery with a combined general and epidural anesthesia.	1998	9773140
inhibition and activation of the thyroidal axis by the adrenal axis in vertebrates.	hormones of the adrenal or interrenal axis and stress situations which induce elevated glucocorticoid plasma levels (e.g. handling and starvation), inhibit thyroid function in growing and adult vertebrates. however, data indicate that during foetal and embryonic development (mammals and birds) or during larval growth and metamorphosis (fish and amphibians), the adrenal axis may stimulate thyroid function. recent findings have provided some information concerning this stimulatory interference of ...	1998	9773500
impaired thyroid function in murine toxoplasmosis.	a decline in serum thyroxine (t4) occurs in nya: nylar female mice infected with toxoplasma gondii. to ascertain whether the hypothyroxinaemia might be the result of primary thyroid dysfunction, 2 parameters of thyrofollicular cell function were monitored to determine (a) if the cell surface membrane receptors for thyroid-stimulating hormone (tsh) were operative, and (b) whether the cyclic adenosine monophosphate (camp)-dependent cascade of intracellular events leading to the release of t4 was r ...	1998	9774785
aetiology of thyroidal dysfunction in murine toxoplasmosis.	mice infected with toxoplasma gondii manifest a rapid decline in serum thyroxine (t4) levels. to locate the locus of the hypothyroxinaemia, the integrity of the pituitary-thyroid axis of infected mice was assessed by a thyrotropin-releasing hormone (trh) assay. a rise in serum t4 after inoculation of trh implies the release of thyrotropin (thyroid-stimulating hormone) from a functionally intact pituitary. administration of a single, large-dose (1 microgram) bolus of trh to infected mice induced ...	1998	9774786
[papillary carcinoma of the thyroid. a uni- and multivariate analysis of the factors affecting the prognosis inclusive of surgical treatment].	a total of 463, out of 677 patients operated on for papillary thyroid carcinoma between 1967-1995, were selected, on the basis of a 5-year minimal follow-up (max 28, mean 11.15 +/- 5.29), for uni and multivariate analysis of survival curves. patient sex, age over and under 45 years, tumour size smaller and larger than 15 mm, stage, any tnm parameter, histological variety, surgical procedure on the thyroid and nodes were the factors assessed in order to express the prognosis.	1998	9774838
[hyperthyroidism in people over 50 years of age in senegal. study of 31 cases observed over a 14-year period].	though common in elderly, with poor and masked symptomatology, hyperthyroidism has never been studied in people older than 50 years of age in african countries.	1998	9775148
aesthesioneuroblastoma.	forty patients were treated or followed up for aesthesioneuroblastoma between 1980 and 1995 at institut gustave roussy, france. there were three t1, seven t2, 15 t3 and 15 t4 lesions. the cervical metastatic rate at presentation was 18 per cent. distant metastases were detected by bone marrow biopsy and bone scan in three patients at presentation. treatment modalities included surgery alone in eight patients, radiotherapy alone in three patients, combined modality surgery plus radiotherapy in 11 ...	1998	9775291
guanine is the target for direct ionisation damage in dna, as detected using excision enzymes.	exposure of an aqueous, aerated solution (ph 7) of a double-stranded dna to 193 nm light, of sufficient energy to ionise dna, leads to selective, non-random modification at guanine in the form of frank single-strand break (ssb) and base modifications, revealed by treatment with either escherichia coli formamidopyrimidine-dna glycosylase (fpg), escherichia coli endonuclease iii (nth) or hot piperidine treatment. there is a similar neighbouring base sequence dependence for fpg- and nth-sensitive d ...	1998	9776756
circular dichroism and uv melting studies on formation of an intramolecular triplex containing parallel ta:t and gg:c triplets: netropsin complexation with the triplex.	we have used circular dichroism and uv absorption spectroscopy to characterize the formation and melting behaviour of an intramolecular dna triple helix containing parallel ta:t and gg:c triplets. our approach to induce and to stabilize a parallel triplex involves the oligonucleotide 5'-d(g4a4g4[t4]c4t4c4-[t4]g4t4g4) ([t4] represents a stretch of four thymine residues). in a 10 mm sodium cacodylate, 0.2 mm disodium edta (ph 7) buffer, we have shown the following significant results. (i) while ...	1998	9776765
[effect of thyroid hormones on the modulation of genetic expression of liver cytosolic malic enzyme, in rats poisoned with hexachlorobenzene].	hexachlorobenzene (hcb) is a widespread environmental pollutant. chronic exposure of laboratory animals to hcb triggers porphyria, induction of liver microsomal enzymes, low levels of t4 reproductive dysfunction's, liver and thyroid tumors. previous findings from our laboratory have shown that hcb increased the activity of the liver thyroid-responsive enzymes: malic enzyme (me), glucose-6-phosphate dehydrogenase (g6pd) without any change in the mytochondrial alpha-glycerophosphate dehydrogenase ...	1998	9777035
the effect of recombinant human thyrotropin (rhtsh) on thyroid function in mice and rats.	the vast majority of studies to determine the biological activity of recombinant human thyrotropin (rhtsh) have been carried out in the mouse. we have recently reported that 0.1 mg of rhtsh im (one-ninth the dose given in thyroid cancer patients) given to normal subjects elicits a brisk rise in serum thyroxine (t4), triiodothyronine (t3), and thyroglobulin (tg) concentrations. in contrast, in initial studies in the rat, a low dose of rhtsh failed to increase serum t4 or t3 concentrations. the pr ...	1998	9777753
kinetic characterization of a bacteriophage t4 antimutator dna polymerase.	fidelity of dna replication by bacteriophage t4 dna polymerase is achieved in a multiplicative process: base selection by its polymerase activity and removal of misincorporated nucleotides by its exonuclease activity. the wild-type polymerase is capable of maintaining a balance between the two activities so that dna replication fidelity is maximized without excessive waste of nucleotides. antimutator enzymes exhibit a higher dna replication fidelity than the wild-type enzyme, at the cost of incr ...	1998	9778349
overexpression of transforming growth factor-beta1 in scirrhous carcinoma of the stomach correlates with decreased survival.	a retrospective study of 50 patients with scirrhous carcinoma of the stomach who had undergone surgery was performed to elucidate whether immunohistochemical overexpression of transforming growth factor-beta1 (tgf-beta1) could predict the patients' survival. to evaluate the proliferative activity of the tumor, the proliferating cell nuclear antigen (pcna) index was used. expression of tgf-beta1 was low in 27 patients and high in 23. clinicopathological characteristics were not significantly diff ...	1998	9778627
[the demonstration of blood flow in focal breast lesions by power-doppler sonography. a new approach to assessment?].	power-doppler sonography is regarded as a very sensitive method for detecting low-velocity and low-volume blood flows. the purpose of our study was to investigate whether increased vascularity in breast carcinoma can be visualized by power-doppler sonography and whether new criteria for differentiating benign and malign lesions can be found.	1998	9779062
bone mineral density by single photon x-ray absorptiometry in chilean children and adolescents.	to assess bone mineralization in healthy chilean children and adolescents, considering sex, age, height, and puberty.	1998	9779858
cerebellar microfolia and other abnormalities of neuronal growth, migration, and lamination in the pit1dw-j homozygote mutant mouse.	the snell dwarf mouse (pit1dw-j homozygote) has a mutation in the pit1 gene that prevents the normal formation of the anterior pituitary. in neonates and adults there is almost complete absence of growth hormone (gh), prolactin (prl), thyroxin (t4), and thyroid-stimulating hormone (tsh). since these hormones have been suggested to play a role in normal development of the central nervous system (cns), we have investigated the effects of the pit1dw-j mutation on the cerebellum and hippocampal form ...	1998	9779941
translation efficiencies of the 5' untranslated region from representatives of the six major genotypes of hepatitis c virus using a novel bicistronic reporter assay system.	the 5' untranslated region (5'utr) of hepatitis c virus (hcv) contains an internal ribosome entry site (ires) which directs translation of the viral open reading frame (orf). the 5'utr is highly conserved between virus isolates in both primary sequence and predicted secondary structure. we cloned and sequenced the 5' regions (nt 18 of the 5'utr to nt 15 of the core coding sequence) of hcv isolates representing the six major genotypes and subcloned these into a bicistronic, dual luciferase report ...	1998	9780040
effects of photoperiod and age on secretory patterns of luteinizing hormone and testosterone and semen production in male domestic turkeys.	the objective of this study was to determine effects of photoperiod and age on the circulating concentrations of lh, testosterone (t), thyroxine (t4), and semen production in male turkeys. male turkeys from 10 or 12 wk of age were maintained under either a long-day (ld) photoperiod of 16l:8d up to 35 wk (ll), or a short-day (sd) photoperiod of 6l:18d to 29 wk and then an ld photoperiod of 16l:8d up to 35 wk of age (sl). plasma concentrations of both lh and t increased by 17 or 18 wk of age under ...	1998	9780324
[locoregional treatment of breast cancer: surgery and radiotherapy].	locoregional control of breast cancer can be achieved with surgery and radiotherapy. surgery and irradiation is advocated for in situ carcinoma and invasive breast cancer. for t4 lesions, breast cancer locoregional treatment must be performed after neoadjuvant therapy. locoregional recurrences without distant metastasis need radical surgery with or without neoadjuvant chemotherapy. locoregional control of breast cancer is a part of the multidisciplinary approach of this disease.	1998	9781210
acute and delayed effects of a single-dose injection of interleukin-6 on thyroid function in healthy humans.	interleukin-6 (il-6) is produced in response to inflammatory and noninflammatory stress and acts as the principal regulator of the acute-phase protein response. il-6 stimulates the hypothalamic-pituitary-adrenal axis and may be involved in the thyroid function abnormalities observed in nonthyroidal illness (nti). this study examined the effects of single-dose il-6 (3 microg/kg subcutaneously [s.c.]) in healthy human subjects: 19 received il-6 and 13 received control saline injection. the dose of ...	1998	9781636
surgical salvage after failed radiation for paranasal sinus malignancy.	to comment on the role of surgical salvage following failed initial treatment for paranasal sinus malignancy.	1998	9818815
identification and characterization of the fourth single-stranded-dna binding domain of replication protein a.	replication protein a (rpa), the heterotrimeric single-stranded-dna (ssdna) binding protein (ssb) of eukaryotes, contains two homologous ssdna binding domains (a and b) in its largest subunit, rpa1, and a third domain in its second-largest subunit, rpa2. here we report that saccharomyces cerevisiae rpa1 contains a previously undetected ssdna binding domain (domain c) lying in tandem with domains a and b. the carboxy-terminal portion of domain c shows sequence similarity to domains a and b and to ...	1998	9819409
contribution of 3,5,3'-tri-iodothyronine produced locally from thyroxine in several maternal tissues of the near-term pregnant rat.	3,5, 3'-tri-iodothyronine(t3)is produced by the thyroid and locally, by monodeiodination of thyroxine (t4), in the peripheral tissues. during pregnancy the thyroid hormone status in rats is altered: plasma and tissue levels of t4 and t3 are decreased. we investigated the effects of pregnancy on the contribution of t3 produced locally in the maternal tissues by administering a continuous infusion of [125i]t4 and [131i]t3. the transport of t4 to almost all maternal organs diminished. less t was tr ...	1998	9820624
seasonal regulation of prolactin secretion in hypophyseal stalk transected beef calves.	seasonal regulation of prolactin secretion was investigated in crossbred beef heifer calves. calves were randomly assigned to hypophyseal stalk transection (hst, n = 6) or sham-operation control (soc, n = 6) groups and fitted 1 day before surgery with an indwelling external jugular catheter. prolactin (prl), growth hormone (gh), thyroid stimulating hormone (tsh), thyroxine (t4), and tri-iodothyronine (t3) in peripheral serum were measured by radioimmunoassay in samples obtained before and after ...	1998	9821500
the role of mutation frequency decline and sos repair systems in methyl methanesulfonate mutagenesis.	methyl methanesulfonate (mms) is an sn2 type alkylating agent which predominantly methylates nitrogen atoms in purines. among the methylated bases 3mea and 3meg are highly mutagenic and toxic. the excision of these lesions leads to the formation of apurinic (ap) sites and subsequently to at-->ta or gc-->ta transversions. the in vivo method based on phenotypic analysis of arg+ revertants of escherichia coli k12 and sensitivity to t4 nonsense mutants has been used to estimate the specificity of mm ...	1998	9821881
the effect of lumbar epidural anesthesia on maternal middle cerebral artery blood flow in normal pregnancy: a prospective, randomized, double-blind comparison study.	lumbar epidural anesthesia is associated with a transient elevation in intracranial pressure in both animals and humans and decreased cerebral blood flow in animals. we sought to determine the effect of medium-onset and slow-onset local anesthetic lumbar epidural anesthesia on maternal cerebral blood flow in normal human pregnancy.	1998	9822508
incidence of port site recurrence after laparoscopic cholecystectomy for preoperatively unsuspected gallbladder carcinoma.	we sought to determine the incidence of recurrence of carcinoma at the port site and the outcome of patients with such recurrences after exploratory laparoscopy/laparoscopic cholecystectomy for unsuspected gallbladder carcinoma and analyzed aspects of the laparoscopic procedure associated with recurrences at the port site.	1998	9823395
resympathectomy for palmar and axillary hyperhidrosis.	the aim was to analyse patterns of failure or symptom recurrence after primary sympathectomy for palmar or axillary hyperhidrosis, and to carry out tactical problem-solving for resympathectomy and review the operative findings.	1998	9823911
intrapulmonary satellite nodule of lung cancer as a t factor.	we conducted a validation of the treatment of satellite nodules in the uicc tnm classification of 1993 and 1997.	1998	9824006
determination of thyroxine in the hair of newborns by radioimmunoassay with high-performance liquid chromatographic confirmation.	hair analysis is often used in forensic toxicology to study, retrospectively, chronic exposure of individuals to drugs, and consequently newborn hair may become an ideal sample to study intrauterine exposure to xenobiotics as well as to endogenous compounds. as a tool to investigate a supposed maternal thyroxine (t4) supply to the congenital hypothyroid fetus, we devised to use the analysis of t4 extracted from newborn hair. in the present paper, the analytical method based on t4 extraction from ...	1998	9824220
thyroid function in lead smelter workers: absence of subacute or cumulative effects with moderate lead burdens.	to evaluate the effect of low to moderate occupational lead exposure on thyroid function we conducted a cross-sectional study of 151 male lead smelter workers.	1998	9826077
the bacteriophage t4 dna packaging apparatus targets the unexpanded prohead.	during the morphogenesis of the bacteriophage t4 capsid, a conformational change of the major head shell protein, gene product (gp) 23, causes a 50% increase in capsid volume. this expansion is required to accept the full length chromosome and, therefore, must precede the completion of packaging. the expanded shell is thinner and more stable than its precursor, and can bind accessory proteins which further stabilize it. in phages lambda, t3, t7 and p22, expansion occurs during dna packaging. how ...	1998	9826505
capsid expansion follows the initiation of dna packaging in bacteriophage t4.	most bacteriophages undergo a dramatic expansion of their capsids during morphogenesis. in phages lambda, t3, t7 and p22, it has been shown that expansion occurs during the packaging of dna into the capsid. the terminase-dna complex docks with the portal vertex of an unexpanded prohead and begins packaging. after some of the dna has entered, the major head protein undergoes a conformational change that increases both the volume and stability of the capsid. in phage t4, the link between packaging ...	1998	9826506
tsh and thyroid hormones induce the release of secretory granules in the harderian gland of hypophysectomized frogs, (rana esculenta): morphological observations.	the secretory activity of the harderian gland (hg) in the frog rana esculenta is influenced by pituitary and thyroid hormones. administration of thyroid stimulating hormone (tsh), t3 and t4 to hypophysectomized male and female frogs, affects the release of secretory granules in the lumina of glandular acini. in particular, treatment with t3 radically modified morphological parameters (height and area of acinar lumina of the glandular cells); t4 or tsh had less effect. administration of thiouraci ...	1998	9827054
sulfation of thyroid hormones by liver of rainbow trout, oncorhynchus mykiss.	we studied hepatic sulfation of thyroid hormones (th) in rainbow trout, oncorhynchus mykiss. sulfation of thyroxine (t4) and 3,5,3'-triiodothyronine (t3) was detected in the cytosolic (63-67%), microsomal (12-16%), nuclear (12-14%) and mitochondrial/lysosomal (7-8%) fractions. using 3'-phosphoadenosine 5'-phosphosulfate (paps) as a sulfate donor, sulfation of t4 and t3 by the cytosolic fraction depended on protein concentration and time. the ph profiles for t4- and t3-sulfation were broad and ov ...	1998	9827058
[non-thyroid indications of treatment with thyroid hormones].	introduction: the use of thyroid hormone is currently strictly limited to thyroid diseases. several recent papers have examined the effects of thyroid hormone in non-thyroidal diseases. these studies examined either the use of the pharmacological properties of thyroid hormone or the effect of the correction of the decrease in triiodothyronine (t3) associated with non-thyroid illnesses. current knowledge and key points: intravenous administration of t3 improves the cardiac index during ongoing ca ...	1998	9827444
inhibitory action of oral thyrotropin-releasing hormone on the glucoregulatory response of the oral glucose tolerance test.	thyrotropin-releasing hormone (trh) has been found in the gastrointestinal tract, where it mainly exerts an inhibitory action. we used oral trh, a stable and powerful formulation, to explore the glucoregulatory response of oral glucose tolerance test (ogtt) on obese patients with impaired glucose tolerance (igt). seven obese patients with igt and eight controls were investigated. three tests were performed on three separate days. on day 1, an oral trh test: a 40 mg trh tablet, was given. blood s ...	1998	9827661
the thyroid axis and depression.	hypothyroidism may give rise to frank depression that usually responds to thyroxine therapy. depressed subjects with subclinical hypothyroidism and/or autoimmune thyroiditis should probably also be treated similarly. most patients with depression, although generally viewed as chemically euthyroid, have alterations in their thyroid function including slight elevation of the serum thyroxine (t4), blunted thyrotropin (tsh) response to thyrotropin-releasing hormone (trh) stimulation, and loss of the ...	1998	9827665
novel hepatoselective insulin analogues: studies with covalently linked thyroxyl-insulin complexes.	to explore the possibility that insulin analogues designed to have restricted access to peripheral tissues may display relative hepatoselectivity in vivo, nalphabeta1-thyroxyl-insulin (b1-t4-ins) and nalphabeta1-thyroxyl-aminohexanoyl insulin (b1-t4-aha-ins) were synthesized. these insulin analogues bind thyroid hormone binding proteins to form high molecular weight complexes. effects of intravenous infusions of b1-t4-ins; b1-t4-aha-ins; combined thyroxine binding globulin (tbg) and b1-t4-ins an ...	1998	9827847
monitoring metal ion flux in reactions of metallothionein and drug-modified metallothionein by electrospray mass spectrometry.	the capabilities of electrospray ionization mass spectrometry are demonstrated for monitoring the flux of metal ions out of and into the metalloprotein rabbit liver metallothionein and, in one example, chlorambucil-alkylated metallothionein. metal ion transfers may be followed as the reactions proceed in situ to provide kinetic information. more uniquely to this technique, metal ion stoichiometries may be determined for reaction intermediates and products. partners used in these studies include ...	1998	9828006
postnatal exposure to chlorinated dioxins and related chemicals on thyroid hormone status in japanese breast-fed infants.	effects of postnatal exposure to polychlorinated dibenzo-p-dioxins (pcdds), polychlorinated dibenzofurans (pcdfs) and coplanar polychlorinated biphenyls (co-pcbs) on thyroid hormone status were studied in the peripheral blood of 36 breast-fed japanese infants. estimated total intakes of these chemicals in toxic equivalent quantity (teq) converted into 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-tcdd) from the breast milk significantly and negatively correlated with the levels of triiodothyronin ...	1998	9828307
change in serum thyroid-stimulating hormone concentration in response to administration of thyrotropin-releasing hormone to healthy dogs, hypothyroid dogs, and euthyroid dogs with concurrent disease.	to determine whether measuring change in serum thyroid-stimulating hormone (tsh) concentration in response to thyrotropin-releasing hormone (trh) administration can be used as a test of thyroid function in dogs suspected of having hypothyroidism.	1998	9828939
clinical and molecular characterization of a brazilian patient with pit-1 deficiency.	we studied a 14 year-old girl with extreme short stature (-9.5 sds), normal psychomotor development and signs of progressive hypothyroidism. basal igf-i and t4 were low. serum gh was low after insulin-induced hypoglycemia and gh-releasing hormone administration. both tsh and prolactin were low and did not rise after trh administration. gonadotropins were normal and cortisol levels were elevated. in contrast, dhea-s levels were low and she did not develop pubic hair until 26 years of age, compati ...	1998	9829213
prognostic implications of pulmonary satellite nodules: are the 1997 staging revisions appropriate?	in the 1992 ajcc and 1993 uicc staging systems, primary lobe satellite nodules increased the t designation of the primary by one level and ipsilateral non-primary lobe satellite nodules raised the t designation to t4. the recent 1997 uicc and ajcc staging revisions assign a t4 (iiib) designation to satellite nodules in a primary lobe, and a m1 (iv) designation to satellites in ipsilateral non-primary lobes. there is abundant evidence showing that satellite nodules are negative prognostic factors ...	1998	9829541
[phase ii study on preoperative radio-chemo-thermotherapy in locally advanced rectal carcinoma].	recent studies show that preoperative radio-chemotherapy can increase resectability and local control of locally advanced rectal carcinomas. additional regional hyperthermia might increase remission rates and tumor response. we therefore tested regional hyperthermia together with radio-chemotherapy in a phase-ii study on locally advanced rectal carcinomas.	1998	9830436
[treatment of basedow-graves' hyperthyroidism: retrospective analysis after 30 years].	it is still debated which is the best treatment for basedow-graves' hyperthyroidism (bgh). we reviewed 195 patients treated and followed-up during the past 30 years: 88 treated with propylthiouracil (ptu), 70 with 131i and 37 thyroidectomized.	1998	9830747
studies of the hormonal regulation of type 2 5'-iodothyronine deiodinase messenger ribonucleic acid in pituitary tumor cells using semiquantitative reverse transcription-polymerase chain reaction.	we developed a sensitive competitive rt-pcr technique for quantitating the ratio of d2 to cyclophilin messenger rna (mrna) and used this to study type 2 deiodinase (d2) mrna regulation. hyperthyroidism in rats causes a 2- to 3-fold reduction in anterior pituitary and medial basal hypothalamus (mbh). thyroid hormone (t3) withdrawal increased the d2/cyclophilin ratio 2- to 3-fold over 48 h in both gc and gh4c1 cells. t3 additional reduced d2 gene transcription by 50% over 2 h and about 30% over th ...	1998	9832426
surgery for thoracic esophageal carcinoma with clinically positive cervical nodes.	we attempted to determine whether and under what circumstances surgical intervention should be recommended for patients with clinically positive cervical nodes for metastasis from thoracic esophageal carcinoma by pretreatment examination.	1998	9832686
cell proliferation and apoptosis of thyroid follicular cells are involved in the involution of experimental non-tumoral hyperplastic goiter.	to assess the involvement of cellular inhibition and the appearance of apoptosis in regression of the hyperplastic thyroid gland towards normality, an experimental design was used to elicit non-toxic goiter by inducing hyperplastic goiter in rats by treatment with methimazole. we performed a morphological and pcna immunocytochemical study together with in situ end labelling with bromodeoxyuridine in thyroid glands of rats receiving methimazole in their drinking water over 21 days after which the ...	1998	9833684
human electroencephalographic (eeg) response to olfactory stimulation: two experiments using the aroma of food.	the present studies sought to examine the effect of olfactory stimulation on human central nervous system activity. in the first experiment (n = 21), eeg response to the 'synthetic' odours of chocolate, spearmint, almond, strawberry, vegetable, garlic and onion, and cumin or no odour was recorded from 19 electrodes (f3, f4, f7, f8, fz, t3, t4, t5, t6, p3, p4, pz, o1, o2, c3, c4, cz) in all eeg frequencies (delta, theta, alpha, beta1 and beta2). exposure to the odour of chocolate was associated w ...	1998	9834885
[influence of neoadjuvant treatment on bladder infiltrating tumors treated with radical cystectomy].	retrospective study of 107 patients diagnosed with infiltrant tumour of the bladder in stage t3-t4 n0-n1, treated with radical surgery. eighty-four (84) received neoadjuvant therapy with radio- and/or chemotherapy. the neoadjuvant treatment was seen to provide significantly better survival, but the specific type of neoadjuvant treatment appears to have no influence. the multivariate study evidenced that the two most influential variables for survival are the complementary treatment and the clini ...	1998	9835083
nasopharyngeal carcinoma in childhood and adolescence.	this study reviews the authors' experience from 1979 through 1996 in the management and outcome of 56 patients with nasopharyngeal carcinoma who were under 20 years of age.	1998	9835902
apnea following spinal anaesthesia in two former pre-term infants.	to report the occurrence of apnea and bradycardia in two former pre-term infants who received spinal anaesthesia without inhalational or intravenous anaesthetic agents during inguinal herniorrhaphy.	1998	9836035
physiological regulation of hypothalamic trh transcription in vivo is t3 receptor isoform specific.	thyroid hormone (tri-iodo-thyronine, t3) exerts transcriptional effects on target genes in responsive cells. these effects are determined by dna/protein interactions governed by the type of t3 receptors (trs) in the cell. as trs show tissue and developmental variations, regulation is best addressed in an integrated in vivo model. we examined tr subtype effects on thyrotropin-releasing hormone (trh) transcription and on the pituitary/thyroid axis end point: thyroid hormone secretion. polyethyleni ...	1998	9837866
[analysis of 33 patients with nasopharyngeal carcinoma--surgical management for persistent tumor after radiation therapy].	we report thirty-three patients treated for nasopharyngeal carcinoma (npc) in our clinic from 1976 to 1997. they consisted of 24 males and 9 females, and their ages ranged from 17 to 76 years with an average of 56 years. fourteen patients initially presented with ear symptoms due to tubal insufficiency, 11 with cervical lymphadenopathy, 6 with nasal symptoms and 4 with cranial nerve paralysis. the mean interval from the onset of their complaints to the time of difinite diagnosis of npc was 5.8 m ...	1998	9838791
[clinical study of hypopharyngeal carcinoma].	a clinical study was performed of 83 patients with hypopharyngeal cancer treated in the sapporo medical university hospital from 1982 to 1995. five-year cumulative survival rate was 34.9% in the whole group and 37.2% in the radical surgical and/or radiation treatment group. in the radiation treatment group, the patients with t1, t2, n0 or n1 stage disease, especially those whose original disease responded almost completely to 40gy irradiation, showed high tumor control rates following full-dose ...	1998	9838796
alkaline heating of canola and rapeseed meals reduces toxicity for chicks.	a simple method for improving the nutritive quality of canola and high glucosinolate rapeseed meals for monogastric animals (chicks) was developed; the meals were mixed with nahco3 and nh4hco3, then heated in a conventional oven. chicks fed untreated canola or rapeseed meals gained less weight than those fed a soybean meal diet, whereas chicks fed the alkaline-heated meals had weight gains not significantly different than those fed the soybean diet. the antithyroid effect of the untreated rapese ...	1998	9839830
staging of oesophageal carcinoma with high resolution endoluminal ultrasonography.	oesophageal carcinoma is a disease with poor prognosis despite improved treatment and diagnostic methods. the most important prognostic features are the degree of wall invasion and the presence of lymph node metastasis. endoscopic ultrasonography (eus) in the region of 7.5-12 mhz has improved the accuracy of tumour staging. in the course of 1 year, 21 patients with oesophageal carcinoma were examined with 20 mhz high-resolution intraluminal ultrasonography (hres). in 11 (52%) patients, both ultr ...	1998	9840517