Publications

[development and use of a method of determination of specific immunoglobulin g based on immunoenzyme analysis in the diagnosis of pulmonary aspergillosis].	enzyme-linked immunoassay (elisa) was employed for determination of specific immunoglobulin g to aspergillus fumigatus (a.f.). culture filtrate of a.f. was used as antigen. optimal conditions of elisa were found. serum of 138 asthmatic patients with total ige level more than 500 pru/ml were analysed for determining the limits of igg to a.f. concentrations.	1992	1421313
efficacy of fluconazole in the treatment of systemic fungal infections.	the efficacy of fluconazole in the treatment of systemic fungal infections was evaluated in an open non-comparative trial. a total of 48 patients with proven or suspected fungal infections were enrolled in 40 of whom efficacy was evaluable. candida albicans accounted for 90% of the infections. candida parapsilosis, candida glabrata, histoplasma capsulatum and aspergillus fumigatus caused the infection in one case each. fluconazole was administered at a dosage of 200-400 mg daily for a mean durat ...	1992	1425709
liposomal hamycin in the control of experimental aspergillosis in mice: relative toxicity, therapeutic efficacy and tissue distribution of free and liposomal hamycin.	therapeutic efficacy of liposomal hamycin has been evaluated in an animal model system for aspergillosis in balb/c mice. hamycin was intercalated into soya phosphatidyl choline (spc), spc: choline (1:1, vol./vol.) and dmpc liposomes. a single dose of either 0.1 mg/kg, 0.25 mg/kg or 0.5 mg/kg of liposomal hamycin and 0.1 mg/kg of free hamycin was injected (i.v.) into animals infected with aspergillus fumigatus. an increase in the survival rate of animals along with decrease in fungal count in var ...	1992	1427960
butyrolactols a and b, new antifungal antibiotics. taxonomy, isolation, physico-chemical properties, structure and biological activity.	new antifungal antibiotics, butyrolactols a and b, have been isolated from the culture broth of streptomyces rochei s785-16. they are novel type of molecules containing a common 2,3-dihydroxybutyrolactone nucleus substituted with a different long hydroxyalkyl side chain. butyrolactol a showed good antifungal activity against aspergillus fumigatus and trichophyton mentagrophytes, and moderately inhibited the growth of yeasts.	1992	1429230
in vitro and in vivo antifungal activities of bms-181184.		1992	1429237
lysine biosynthesis in selected pathogenic fungi: characterization of lysine auxotrophs and the cloned lys1 gene of candida albicans.	the alpha-aminoadipate pathway for the biosynthesis of lysine is present only in fungi and euglena. until now, this unique metabolic pathway has never been investigated in the opportunistic fungal pathogens candida albicans, cryptococcus neoformans, and aspergillus fumigatus. five of the eight enzymes (homocitrate synthase, homoisocitrate dehydrogenase, alpha-aminoadipate reductase, saccharopine reductase, and saccharopine dehydrogenase) of the alpha-aminoadipate pathway and glucose-6-phosphate ...	1992	1429460
selective expression of a major allergen and cytotoxin, asp f i, in aspergillus fumigatus. implications for the immunopathogenesis of aspergillus-related diseases.	asp f i is a major 18-kda aspergillus fumigatus allergen and a member of the mitogillin family of cytotoxins. the nucleotide sequence of the asp f i gene was determined by sequencing polymerase chain reaction products amplified from a. fumigatus spore dna. the entire 678-bp dna includes an 81-bp leader sequence, preceding the n-terminal alanine codon, a 52-bp intron, and a 444-bp open reading frame, encoding a 149-amino acid protein (m(r) 16,899), which is 99% homologous to mitogillin from asper ...	1992	1431110
factors associated with invasive lung aspergillosis and the significance of positive aspergillus culture after liver transplantation.	from january 1981 to december 1990, 2180 patients underwent orthotopic liver transplantation at the university of pittsburgh. thirty-two patients (1.5%) were identified with invasive aspergillosis (29 lung, 2 intraabdominal, 1 meningitis). of 29 patients with invasive lung disease, only 23 (79%) had positive culture (aspergillus fumigatus, 20; aspergillus flavus, 3). forty-eight variables were analyzed and compared in 23 patients with invasive disease with positive cultures and 9 patients with c ...	1992	1431256
allergic aspergillus sinusitis with proptosis.	allergic aspergillus sinusitis has recently been increasingly recognized. five cases are discussed. all presented with proptosis, signs and symptoms of allergic rhinitis and radiological evidence of expansile masses with calcification and bony erosions involving multiple sinuses. greenish cheesy material was seen at surgery. histologically the lesions contained eosinophils, charcot-lyden crystals and fungal septate hyphae. aspergillus fumigatus was grown from all cases. surgical removal with dra ...	1992	1431517
cerebral infarction due to aspergillus arteritis following glioma surgery.	cerebral infarction due to fungal arteritis is an uncommon complication of neurosurgical operations and adjuvant immunosuppressive therapy, including long-term steroids. if unrecognized, the neurological deterioration which ensues may be mistreated by increasing the dose of steroids. a case of a 38-year-old caucasian male who had no obvious immune deficiency or fungal infection prior to a craniotomy for cerebral tumour is described in whom perioperative aspergillus infection resulted in cerebral ...	1992	1449673
lack of vessel wall elastolysis in human invasive pulmonary aspergillosis.	in experimental studies, the apparent ability of aspergillus fumigatus isolates to produce elastase in agar plates correlates with their ability to cause invasive pulmonary aspergillosis in mice pretreated with cortisone. thus, elastase production may govern the pathogenicity of particular isolates. if this is so, then disruption of the elastic layers within blood vessel walls in invasive aspergillosis would be expected. to test this hypothesis, tissue blocks were prepared from nine patients wit ...	1992	1452348
[chronic eosinophilic pulmonitis with eosinophilic pleurisy. a report on 2 clinical cases seen by the authors].	we discuss the cases of two patients affected with chronic eosinophilic pneumonia (cep) pleurisy and eosinophilia in pleural effusion, not previously mentioned in the literature, to point out their peculiarity, to consider differential diagnosis and the effect of steroid therapy. both patients, a 57-year-old man and a 55-year-old woman, were atopic: they had been suffering from allergic rhinitis and asthma for several years when they suffered sudden onset of cough, dyspnea and thoracic pain. thi ...	1992	1457257
[56-year-old patient with recurrent lung infiltration and "lung tumor"].		1992	1461222
immunopathology of sids.	immunopathological studies of sids share the problems of all necropsy based studies of this syndrome: the extent of autolytic changes in the material under study; and the lack of appropriate controls. despite these problems, several studies have been performed on serum, bronchoalveolar lavage, and pulmonary tissue. many of these studies have been inspired by the modified anaphylaxis hypothesis, based on the experiments of coombs and coworkers. lightly anaesthetised guinea-pigs, which had been se ...	1992	1474158
allergic bronchopulmonary mycosis complicating cystic fibrosis.	allergic bronchopulmonary aspergillosis (abpa) is a hypersensitivity lung disease caused by bronchial colonization with aspergillus fumigatus that affects approximately 10% of patients with cystic fibrosis (cf). the diagnosis in cf patients is difficult because the cardinal symptoms of abpa occur frequently in cf, ie, pulmonary infiltrates and wheezing, as well as the frequent colonization with a fumigatus that leads to humoral reactivity. if left untreated, abpa leads to bronchiectasis and pulm ...	1992	1475542
surgical wound infection by aspergillus fumigatus in liver transplant recipients.	cutaneous aspergillosis is generally associated with immunosuppression, burns, and major trauma. most cases are acquired by direct inoculation, although cutaneous involvement does occasionally occur with disseminated disease. surgical wound infections caused by aspergillus species are very unusual and to our knowledge have not been described in the setting of organ transplantation. we describe two liver transplant recipients who developed wound aspergillosis during a nosocomial outbreak of asper ...	1992	1478051
rhinocerebral aspergillosis.	aspergillosis is increasingly being recognised as a common fungal infection of the paranasal sinuses. although the disease is almost endemic in neighbouring sudan, there are few reported cases from the kingdom of saudi arabia. we report four cases of sinus aspergillosis with involvement of the skull bases and/or intracranial spread; a condition we have termed rhinocerebral aspergillosis. invasive aspergillosis in our subgroup of patients occurs in otherwise healthy patients with normal immune st ...	1992	1479276
saperconazole therapy in a rabbit model of invasive aspergillosis.	the efficacy of orally and intravenously administered saperconazole against aspergillus fumigatus was assessed in an immunosuppressed temporarily leukopenic rabbit model of invasive aspergillosis and compared with that of amphotericin b. oral saperconazole at dosages of 5, 10, and 15 mg/kg of body weight per day improved survival compared with that of controls. in addition, saperconazole at 10 and 15 mg/kg/day reduced the tissue burden and reduced levels of circulating antigen, which correlated ...	1992	1482137
increased prevalence of igg-induced sensitization and hypersensitivity pneumonitis (humidifier lung) in nonsmokers exposed to aerosols of a contaminated air conditioner.	specific igg antibodies against antigens of a contaminated air conditioner were estimated in serum of 134 workers of a printing company. altogether 64% of the workers investigated revealed significantly elevated levels (> 3 u/ml) of igg antibodies specific to these antigens as compared to a nonexposed control group. the occurrence of igg antibodies for microbial extracts were 25% for fusarium, 23% for penicillium notatum, 13% for alternaria tenuis, 12% for aureobasidium pullulans, 9% for sphaero ...	1992	1485005
[surgical treatment of bilateral pulmonary aspergillosis].		1992	1488446
invasive pulmonary aspergillosis with pneumopericardium and pneumothorax.	a case of an invasive pulmonary aspergillosis with pneumopericardium and pneumothorax in an immunocompromised child of 5 years is reported. he was treated with thoracic drainage, amphotericin b and itraconazole. the course was satisfactory.	1992	1491941
allergic bronchopulmonary aspergillosis: a review.		1992	1494165
aspergillus endophthalmitis: a clinical and experimental study.		1992	1494826
an aspergillus fumigatus alkaline protease mutant constructed by gene disruption is deficient in extracellular elastase activity.	invasive pulmonary aspergillosis, usually caused by aspergillus fumigatus, is a life-threatening condition of immunosuppressed patients. we have created a mutant strain of this fungus that lacks an extracellular alkaline protease (afalp). this was accomplished by transformation of a. fumigatus with a plasmid containing a selectable marker for hygromycin b resistance, and a 504 bp segment of the afalp gene, obtained by polymerase-chain-reaction-based amplification of a. fumigatus genomic dna. app ...	1992	1495393
sphingofungins a, b, c, and d; a new family of antifungal agents. i. fermentation, isolation, and biological activity.	in screening for antifungal inhibitors from fungi, four related antifungal agents have been isolated from the cultivation of aspergillus fumigatus atcc 20857. these agents were initially produced by the microorganism growing on a solid millet-based medium. a liquid medium containing both glucose and glycerol has also been developed in which these antibiotics are produced in two phases. these novel compounds, sphingofungins a, b, c, and d, show a limited spectrum of antifungal activity but were e ...	1992	1500351
application of ubiquinone systems and electrophoretic comparison of enzymes to identification of clinical isolates of aspergillus fumigatus and several other species of aspergillus.	the ubiquinone systems and electrophoretic comparison of enzymes were used to determine the relatedness among 64 isolates of seven aspergillus spp. these were 31 clinical and 3 nonclinical isolates of aspergillus fumigatus fres., 2 isolates of a. nidulellus samson & w. gams, 8 isolates of a. terreus thom, 4 isolates of a. flavus link, 1 isolate of a. oryzae (ahlburg) cohn, 14 isolates of a. niger van tieghem, and 1 isolate of a. japonicus saito. the enzymes glucose 6-phosphate dehydrogenase, lac ...	1992	1500506
participation of complement in the phagocytosis of the conidia of aspergillus fumigatus by human polymorphonuclear cells.	nonspecific immunity plays a major role in the clearance of the opportunistic fungal pathogen, aspergillus fumigatus. however, the mode of recognition of aspergillus conidia by the different elements of an intact phagocyte system remains poorly understood. this study concentrated on the initial interaction between infective conidia and human polymorphonuclear cells (pmnl) using a double-label immunofluorescent method. at 37 degrees c, association was rapid. although ingestion rates were slower t ...	1992	1500740
disseminated fungal disease resistant to fluconazole treatment in a child with leukemia.	during a chemotherapy induced leukopenic period fluconazole (3 mg/kg/day i.v.) was administered as empiric antifungal treatment in a 5-year-old girl with leukemia and a presumed catheter infection due to staphylococcus epidermidis. despite intensive treatment with antibiotics and fluconazole the patient died. in one blood culture candida krusei was isolated post mortem, and at autopsy aspergillus fumigatus was found in multiple organs. both fungi showed high mic values to fluconazole. we feel th ...	1992	1509246
[percutaneous treatment of a mycotic renal abscess, caused by aspergillus fumigatus, in an aids patient].	presentation of one case of renal mycosis by aspergillus as a primary complication in one patient diagnosed with acquired immunodeficiency syndrome. the importance of conservative treatment at all costs through percutaneous drainage is underlined, specially if we considered the short life expectancy of patients who have developed aids.	1992	1509920
effects of amino-terminal extensions and specific mutations on the activity of restrictocin.	the cytotoxic activities of restrictocin with aminoterminal extensions and specific mutations were investigated using in vivo and in vitro systems. genes were constructed from the cdna clone of restrictocin which encode: the native form of restrictocin (including the leader sequence); met-prorestrictocin, in which a codon for methionine was placed before a putative pro region; met-mature restrictocin, with a methionine codon prior to the mature form of restrictocin; and three mutated forms of me ...	1992	1512222
regulation of restrictocin production in aspergillus restrictus.	the production of restrictocin (a cytotoxin that specifically cleaves ribosomal rna) by cultures of aspergillus restrictus grown in liquid medium was investigated. the function of restrictocin, the method of its accumulation and the mode of resistance to restrictocin in a. restrictus are unknown. previous studies have indicated that restrictocin accumulates in the medium with culture age. these observations have been extended in this study by cloning the cdna of the res gene and using this cdna ...	1992	1512574
production and localization of restrictocin in aspergillus restrictus.	the production and secretion of restrictocin (a cytotoxin that cleaves ribosomal rna) by cultures of the fungus aspergillus restrictus was investigated. previous studies have indicated that restrictocin production in liquid culture coincides with the appearance of differentiated cell structures. a study of the correlation between the appearance of differentiated structures and restrictocin production was conducted with a. restrictus grown on agar medium. restrictocin was found to be associated w ...	1992	1512575
treatment of cerebral aspergillosis after liver transplantation.	we report the treatment of cerebral aspergillosis with amphotericin b, flucytosine, surgery, and liposomal amphotericin b (l-amb) after a liver transplant. the patient died 2 months after cessation of antifungal therapy, as a consequence of multiple-system organ failure. the only relevant postmortem finding in the brain was a small, encapsulated abscess containing hyphae. this case indicates that l-amb is an effective alternative drug for cerebral aspergillosis.	1992	1513474
ct findings in paranasal aspergillosis.	computed tomographic (ct) scan of eight cases of paranasal sinus aspergillosis were reviewed. different ct patterns were observed such as areas of high densities, linear interlacing network of high density, radiolucent thin rim periphery to the masses, calcification, expansion of involved sinuses and bone erosion. two cases of invasive type showed extension beyond the sinuses into the orbit and intracranially simulating a malignant tumour. the findings are similar to those described by previous ...	1992	1516341
primary cutaneous aspergillosis near central venous catheters in patients with the acquired immunodeficiency syndrome.	aspergillosis in patients with the acquired immunodeficiency syndrome is unusual, and the clinicopathologic features of primary cutaneous aspergillosis in this setting are undefined. our findings show that the manifestations can differ from those of primary cutaneous aspergillosis in other immunocompromised patients.	1992	1519937
fungal diseases of the nasal cavity of the dog and cat.	fungal infections of the nasal cavity are a common cause of nasal disease in the dog and cat. aspergillus fumigatus most commonly affects the dog; cryptococcus neoformans is the most common fungus isolated from the cat. rhinosporidium infection causes obstructive nasal polyps in the dog but has not been reported in the cat. several other miscellaneous fungi, including exophiala, alternaria, trichosporon, blastomyces, and histoplasma, and the alga prototheca occasionally cause nasal disease in do ...	1992	1523784
immunodiffusion test for diagnosing basidiobolomycosis.	an immunodiffusion test was developed for the diagnosis of basidiobolomycosis. when culture filtrate antigen (cfa) from basidiobolus ranarum was reacted against two human patient and two rabbit antisera, 2 precipitin bands, inner (n) and outer (y), were revealed for both patient and rabbit antisera. a line of identity was also observed between precipitin bands obtained with patient and rabbit sera. when cfa from b. ranarum (b cfa) was reacted against rabbit sera which contained antibody to conid ...	1992	1528227
aspergillosis in camels affected with a specific respiratory and enteric syndrome.	during the summer of 1990 deaths, occurred in racing camels (camelus dromedarius) associated with a specific disease syndrome. clinical signs included pyrexia, coughing, lachrymation, oedema of the throat and submandibular region and enlargement of submandibular lymph nodes. in terminal cases nervous signs were present and sometimes there was bloody diarrhoea and vomiting. of 480 camels at least 70 animals were affected with the disease and about 40 died. morbidity and mortality was greater in c ...	1992	1530552
precipitins and specific igg antibody to aspergillus fumigatus in a chest unit population.	the enzyme linked immunosorbent assay (elisa) for detecting igg antibodies to aspergillus fumigatus is more sensitive than the measurement of aspergillus precipitins. the relation of the results from both techniques to the clinical pattern of disease in a large unselected group of patients from a large referral centre is unknown.	1992	1539145
bowel infarction as the initial manifestation of disseminated aspergillosis.	disseminated aspergillosis in the immunocompromised patient most commonly presents with clinically apparent pulmonary involvement and roentgenographic infiltrates. we report a patient with acute myelogenous leukemia who developed bowel infarction due to gastrointestinal invasion of aspergillus fumigatus as the initial manifestation of widespread fungal disease.	1992	1541172
purification and properties of the elastase from aspergillus fumigatus.	elastase, a potential virulence factor from the opportunistic pathogen aspergillus fumigatus, was purified 220-fold from culture broth by fast-performance liquid chromatography employing anion exchange (q sepharose fast flow), cation exchange (s sepharose fast flow), and gel filtration (superose 12). purified to near homogeneity, the elastase had an apparent molecular mass of 32 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (silver stain) but a mass of about 19.1 kda as determ ...	1992	1541545
inhibition of aspergillus fumigatus elastase with monoclonal antibodies produced by using denatured elastase as an immunogen.	in preparing monoclonal antibodies to the elastase from aspergillus fumigatus, we found that the enzyme was weakly immunogenic in balb/c mice. antiserum titers were only 1:1,000 to 1:5,000, and hybridomas secreted nonspecific immunoglobulin m (igm). denaturing the elastase in 0.5% sodium dodecyl sulfate at 80 degrees c for 10 min prior to injection increased titers of antiserum against the nondenatured (native) enzyme 10-fold. of eight hybridomas selected following immunization with the denature ...	1992	1541546
[a case report of pulmonary infiltration with eosinophilia syndrome induced by candida albicans].	a sixty six-year-old female who had been treated for bronchial asthma for about 25 years was admitted to the hospital with complaints of episodes of dyspnea, eosinophilia and infiltrative shadows in the chest x-ray film. an infiltrative shadow appeared to move from the left to the right lung field and finally formed a shadow of atelectasis in the middle field of the right lung. a sputum culture showed only candida albicans. allergic and immunologic examination revealed high ige serum levels with ...	1992	1554325
pathologic and immunocytochemical studies of morbillivirus infection in striped dolphins (stenella coeruleoalba).	hundreds of striped dolphins (stenella coeruleoalba) died along the spanish mediterranean coast during the second half of 1990. we necropsied 58 dolphins. partial collapse of the lungs with patchy atelectasis, subcutaneous edema, icterus, and stomatitis were the most prominent gross morphologic changes. histologically, a bronchiolo-interstitial pneumonia was the most frequent lesion (72% of the animals). it was characterized by hyperplasia of alveolar epithelial type ii cells and formation of mu ...	1992	1557861
renal aspergilloma: an unusual cause of infection in a patient with the acquired immunodeficiency syndrome.	the case of a 36-year-old man with the acquired immunodeficiency syndrome (aids) and a renal aspergilloma is reported. aspergillus infections are uncommon in patients with aids. isolated renal aspergillomas have rarely been reported in the non-aids population (14 cases) and have never been reported in a patient with aids. the patient we describe was clinically symptomatic and initially treated medically, but he did not respond to intravenous amphotericin and oral itraconazole. he eventually requ ...	1992	1558091
comparative efficacy of contact lens disinfection solutions.	using the d value method of analysis, we evaluated the relative antimicrobial effectiveness of eight contact lens disinfection solutions against a standard population of challenge organisms as defined in the microbiological guidelines proposed by the food and drug administration (fda). six bacteria (escherichia coli, staphylococcus aureus, s. epidermidis, streptococcus pneumoniae, pseudomonas aeruginosa, and serratia marcescens) and two fungi (aspergillus fumigatus, candida albicans) were invest ...	1992	1559286
[fungal myocarditis in acquired immunodeficiency syndrome].	the authors report 8 cases of fungal myocarditis discovered at autopsy of a series of 118 patients with aids. the cardiac disease was symptomatic and responsible for death in 2 cases. antemortem diagnosis of fungal infection was made on examination of bronchioloalveolar lavage, the cerebrospinal fluid or gastrointestinal biopsy. the demonstration of intramyocardial pathogens was postmortem in all cases. the organism was candida albicans in 3 cases, cryptococcus neoformans in 3 cases, and aspergi ...	1992	1562224
specific binding of human fibrinogen fragment d to aspergillus fumigatus conidia.	the interaction of purified human fibrinogen with aspergillus fumigatus conidia was investigated by immunofluorescence and electron microscopy and binding assays with radiolabeled proteins. we described the localization of the binding sites on the a. fumigatus conidia and on the fibrinogen molecule and determined the binding characteristics. immunofluorescence revealed that the fixation of purified fibrinogen was selectively associated with conidia and suggested a role for the d domains of the f ...	1992	1563760
interactions between conidia of aspergillus fumigatus and human complement component c3.	activation and deposition of c3 on aspergillus fumigatus conidia have been previously demonstrated. this study investigated in further detail the interactions between complement component c3 and the conidia of a. fumigatus. immunoblotting and 125i-c3 binding studies showed that c3 deposition was rapid (less than 15 min) and parallel to the formation of ic3b. immunoblotting experiments identified a 54- to 58-kda conidial protein which binds human complement component c3 and/or a c3 fragment(s). 1 ...	1992	1563783
aspergillus antigen-induced eosinophil differentiation in a murine model.	eosinophilia is a prominent feature of the cellular response in allergic and parasitic diseases. allergic bronchopulmonary aspergillosis due to colonization of the lungs of some asthmatics with aspergillus fumigatus is characterized by high levels of serum immunoglobulin e and peripheral blood (pb) and lung eosinophilia. this study investigates the role of eosinophils in the pathogenesis of allergic bronchopulmonary aspergillosis by using a mouse model. balb/c mice were immunized intranasally an ...	1992	1563787
aspergillus fumigatus, a rare cause of fatal coronary artery occlusion.	endocarditis by aspergillus species in patients without prior cardiovascular surgery is extremely rare and difficult to diagnose. we report and discuss a 69-year-old patient with hairy cell leukemia who developed severe bilateral pneumonia and metastatic subcutaneous nodules from which a. fumigatus was cultured. he died after 18 days of treatment with an adequate dose (0.7 mg/kg/day) of amphotericin b intravenously. fungal endocarditis and a myocardial infarction due to a septic thrombotic occlu ...	1992	1563813
syngeneic marrow transplantation in multiple myeloma.	two cases of iga multiple myeloma treated with syngeneic bone marrow transplant and a non-total body irradiation (tbi) conditioning regimen are reported. both patients presented with stage iii disease which responded to standard chemotherapeutic management. the myelo-ablative program for transplant consisted of busulfan and cyclophosphamide (bu/cy). evaluation at 40 days post-transplant revealed no evidence of residual myeloma in either patient. one patient died of disseminated aspergillus fumig ...	1992	1564944
fatal esophageal aspergilloma in a leukemic adolescent.		1992	1565546
aortic bypass graft infection due to aspergillus: report of a case and review.	aspergillus is a rare cause of aortic graft infection. a recent case is reported and a review of seven other cases described in the literature since 1966 is presented. infections of both thoracic and abdominal aortic grafts have been reported. infection occurred from 5 weeks to 3 years after surgery. underlying immunosuppressive disorders were not present. the most common presenting symptoms were back pain, fever, and embolic phenomena. pseudoaneurysm of the vascular prosthesis with contiguous v ...	1992	1576288
lanomycin and glucolanomycin, antifungal agents produced by pycnidiophora dispersa. i. discovery, isolation and biological activity.	the antifungal agents lanomycin and glucolanomycin were isolated from pycnidiophora dispersa. the compounds were active against species of candida and dermatophytes but were inactive against aspergillus fumigatus and gram-positive and gram-negative bacteria. the compounds inhibited the cytochrome p-450 enzyme lanosterol 14 alpha-demethylase, and are believed, therefore, to have a mode of action similar to the azole and bis-triazole class of antifungal agents.	1992	1577658
an immortalized cell line expresses properties of activated microglial cells.	murine cultured microglial cells were immortalized after infection with a v-raf/v-myc recombinant retrovirus. this immortalized cell line (bv-2) shares properties with body macrophages with respect to the antigen profile, their phagocytic capacity and antimicrobial activity. bv-2 cells are not constitutively able to kill tumor cells in vitro, but acquire antitumor activity following an increase in [ca++]i. bv-2 cells, like microglial cells, are however, distinct from peripheral macrophages by th ...	1992	1578513
invasive aspergillus infections in patients with a malignancy: description of an outbreak and overview of the literature.	improved results can be obtained by increasing the intensity of chemotherapy in patients with cancer. prolonged granulocytopenia due to intensive chemotherapy schedules, however, is a frequent cause of infectious complications. fungal infections, especially aspergillus infections, are being recognized with increasing frequency. we describe an outbreak of invasive aspergillus infections in six cancer patients treated with chemotherapy and we give an overview of the diagnostic and therapeutic dile ...	1992	1579187
concanavalin a-nonbinding aspergillus fumigatus antigen: a major immunogen in allergic bronchopulmonary aspergillosis.	humoral immune responses in allergic bronchopulmonary aspergillosis (abpa) have been well studied. however, reports of cell-mediated immune responses in abpa are conflicting and not well documented, perhaps because well-characterized antigens are not available. in the present study, we assessed the role of peripheral blood mononuclear cells (pbmcs) from patients with abpa in their ability to respond to both crude and semipurified aspergillus fumigatus antigens by in vitro proliferation and immun ...	1992	1583388
mental status changes in an immunocompromised patient.		1992	1587021
liposomal amphotericin-b (ambisome) for treatment of disseminated fungal infections in two infants of very low birth weight.		1992	1594386
aspergillus epidural abscess and cord compression in a patient with aspergilloma and empyema. survival and response to high dose systemic amphotericin therapy.	a 57-yr-old man with a chronic lung cavity presumed to be related to ankylosing spondylitis and/or old cavitary tuberculosis presented with hemoptysis and rapidly developed lower extremity paresis and hypoesthesia. on chest radiograph he had a left upper lobe lesion suggestive of aspergilloma combined with a large left empyema with bronchopleural fistula. serologic analysis demonstrated precipitins and very high titer igg antibodies to aspergillus fumigatus antigens. decompressive laminectomy fr ...	1992	1596022
class specific antibodies in serodiagnosis of farmer's lung.	the aim of the present study was to determine which microbes and which immunoglobulin (ig) classes should be included in tests to discriminate between patients with farmer's lung and reference persons. the sera of a group of farmer's lung patients and their spouses were measured for igg, iga, igm, and ige antibodies against a panel of farmer's lung microbes. the concentrations of igg, iga, and ige antibodies were higher in patients compared with their spouses. the patients were generally positiv ...	1992	1599871
nucleotide sequence of a genomic and a cdna clone encoding an extracellular alkaline protease of aspergillus fumigatus.	an aspergillus fumigatus extracellular alkaline protease (alp) which is an enzyme of the subtilisin family is a potential virulent factor of the fungus. the gene encoding alp was isolated from a genomic library made from dna of an a. fumigatus isolate. the nucleotide sequence of this gene was compared to that of a cdna encoding a. oryzae alp and to that of a cdna from a. fumigatus encoding the mature alp protein. mature a. fumigatus alp contains 282 amino acids and is encoded by three exons. the ...	1992	1601287
a murine model of allergic bronchopulmonary aspergillosis with elevated eosinophils and ige.	a model of allergic bronchopulmonary aspergillosis was developed by exposing balb/c mice to aspergillus fumigatus (af) ag. animals immunized intranasally (i.n.) with soluble af ag produced low levels of serum ige compared to animals given alum precipitated af ag i.p. the latter treatment also produced higher levels of serum igg1 and af-specific igg1 than soluble af given i.p. or i.n.. blood and lung eosinophilia was detected in mice repeatedly exposed to af by i.n. but not in the groups injected ...	1992	1602128
effect of methotrexate on the germination and growth of aspergillus fumigatus and aspergillus flavus strains.	methotrexate at concentrations of greater than or equal to 10(-5) m caused a significant reduction in the rate of germ tube formation of spores of aspergillus fumigatus, but not of aspergillus flavus, after 7 h incubation. at 10(-3) m the drug caused a significant reduction in germ tube elongation of a. fumigatus and a. flavus strains during the first 7 h of germination. after 18 h incubation, drug concentrations of greater than or equal to 4 x 10(-4) m produced greater than 80% reduction in gro ...	1992	1607326
aspergillus fumigatus: identification of 16, 18, and 45 kd antigens recognized by human igg and ige antibodies and murine monoclonal antibodies.	the immunochemical properties of antigens produced by aspergillus fumigatus were investigated with biochemical purification techniques in conjunction with the production of murine monoclonal antibodies (mabs) and binding studies with human igg and ige antibodies. a. fumigatus antigens were partially purified by gel filtration and hydrophobic interaction chromatography on phenyl-sepharose. two fractions that eluted with either 2 mol/l or 0.15 mol/l of nacl demonstrated strong binding to human igg ...	1992	1607551
evaluation of clinico-immunological parameters in pediatric abpa patients.	allergic bronchopulmonary aspergillosis patients (ten in the age group 5-13) were studied with respect to clinical and immunodiagnostic tests. all the patients showed wheal and flare reaction with a. fumigatus antigens. clinical features included bilateral infiltration and hilar lymphadenopathy. eosinophilic count in these patients was observed to be in the range of 300-2500/mm3. sera of six patients indicated precipitin reaction with a. fumigatus antigens. an elevated total serum ige was notice ...	1992	1612654
fungi associated with bovine abortion in the northern plains states (usa).	mycotic infection was diagnosed in 6.8% of 6,858 cases of bovine abortion and stillbirth examined during a 9-year period. aspergilli were associated with approximately 5% of all abortion cases and 71% of 446 cases that were cultured for fungi and diagnosed as mycotic abortion. aspergillus fumigatus was the most frequent isolate (62%), followed by a. terreus (6.7%), emericella (aspergillus) nidulans (3.0%), a. flavus (2.9%), and e. rugulosus (less than 1.0%). zygomycetes (absidia, mortierella, rh ...	1992	1616983
cloning and expression of recombinant aspergillus fumigatus allergen i/a (rasp f i/a) with ige binding and type i skin test activity.	aspergillus fumigatus secretes an 18-kda nonglycosylated ige-binding protein. this protein was previously shown to be a ribotoxin, like alpha-sarcin and mitogillin. a 686-bp long a. fumigatus cdna encoding an 18-kda ribotoxin was cloned and expressed in escherichia coli as a fusion protein with six adjacent histidines (rasp f i/a). rasp f i/a was purified to homogeneity by ni(2+)-chelate affinity chromatography and refolded. the recombinant protein was enzymatically active resulting in the cleav ...	1992	1624793
nonresolving pneumonia in steroid-treated patients with obstructive lung disease.	to review autopsy-proven cases of opportunistic pneumonia and determine how many of these patients had received corticosteroid therapy for obstructive lung disease in order to define whether this therapy was the major risk factor predisposing to infection.	1992	1626569
monoclonal antibodies bind identically to both spores and hyphae of aspergillus fumigatus.	immunoelectron microscopy (iem) was used to determine the binding of six monoclonal antibodies (moabs) produced against aspergillus fumigatus antigens present on or within the conidia and hyphae of the fungus. antigen-antibody complexes were demonstrated in em using labelled colloidal gold particles (15 nm). three out of 6 moabs (c9, f12 and h10) reacted only with the cytoplasmic components of a. fumigatus while the remaining three (b12, f6g5 and d6e6) showed reactivity to both cytoplasm and cel ...	1992	1628253
neurosurgical infections in the compromised host.	granulocytopenia, cellular and humoral mediated immune dysfunction are predisposing factors to the development of cns infections in immunosuppressed patients. brain abscess formation in the compromised host is most commonly caused by enteric bacilli, the bacterium n. asteroides, the fungus aspergillus fumigatus, or the parasite t. gondii. mucor and the jc virus cause invasive encephalitis in this patient population. ct and mr imaging have greatly aided in the diagnosis of intracranial lesions be ...	1992	1633469
successful use of liposome encapsulated amphotericin to treat invasive aspergillosis following failure of conventional amphotericin.	we describe a case of proven invasive pulmonary aspergillosis in a neutropenic patient in whom disease progression occurred during treatment with conventional amphotericin b despite neutrophil recovery. treatment with liposomal encapsulated amphotericin b resulted in clinical and radiological improvement and the clearance of aspergillus spores from the sputum.	1992	1633682
aspergillus fumigatus fungaemia and myocarditis in a patient with acquired immunodeficiency syndrome.	necrotizing myocarditis due to aspergillus fumigatus was a contributory cause of death in a patient with acquired immunodeficiency syndrome and non-hodgkin lymphoblastic malignant lymphoma of the burkitt type. a transient remission of the lymphoma had been obtained by cytostatic treatment. a. fumigatus was isolated from blood two weeks before death, but myocarditis was not diagnosed until autopsy.	1992	1642847
purification and characterization of a fibrinogenolytic serine proteinase from aspergillus fumigatus culture filtrate.	a fibrinogenolytic proteinase has been isolated from aspergillus fumigatus culture filtrate by ammonium sulfate precipitation followed by successive chromatographies on sephadex g-75 and immobilized phenylalanine. the purified proteinase exhibited a molecular weight of about 33 kda. when analysed by sds-polyacrylamide gels containing co-polymerized fibrinogen, the proteinase appeared as a broad band at the top of the gels, which could correspond to polymerization of the enzyme, as suggested by s ...	1992	1644203
activity of recombinant mitogillin and mitogillin immunoconjugates.	a synthetic gene for the aspergillus protein toxin mitogillin has been synthesized and expressed in escherichia coli. the recombinant mitogillin is a potent inhibitor of protein synthesis in vitro with an ic50 of 9.7 pm. immunoconjugates of recombinant mitogillin derivatized with s-acetylmercaptosuccinic anhydride and 5-methyl-2-iminothiolane modified h65 antibody kill t cell lines and peripheral blood mononuclear cells expressing the human cd5 surface antigen. native mitogillin contains 4 cyste ...	1992	1644844
[evaluation of an elisa for the detection of anti-aspergillus fumigatus antibodies].	an indirect microelisa system for the detection of anti-aspergillus fumigatus antibodies using human anti-igg conjugated to peroxidase was standardized. serum samples of two patients having an aspergilloma diagnosed by clinical, microbiological, and serological criteria were used as positive controls. in addition, 119 serum samples of blood donors and 216 serum samples of patients having chronic pneumopathies were also studied. the standardized system may be used as a diagnostic complement for i ...	1992	9768220
effect of administration of diethylcarbamazine on experimental bacterial and fungal infections in mice.	other researchers have found that diethylcarbamazine (dec) is effective treatment for filariasis despite a lack of demonstrated in vitro antifilarial activity. the results of our previous investigations using feline and murine leukemia virus models encouraged us to investigate the use of dec with other infections. in the current experiments, dec treatments was associated with (a) increased survival and decreased brain streptococcus pneumoniae levels following s. pneumoniae challenge in previousl ...	1992	18611510
effect of administration of diethylcarbamazine with and without fluconazole on experimental fungal infections in mice.	other researchers have found that diethylcarbamazine (dec) is effective treatment for filariasis despite a lack of demonstrated in vitro antifilarial activity. the results of our previous investigations using feline and murine leukemia virus models encouraged us to investigate the use of dec with other infections. in our current experiments, dec treatment was associated with lower brain fungal burden in fluconazole-treated mice following intravenous injection of aspergillus fumigatus or increasi ...	1993	18611537
production of xylanases byaspergillus fumigatus andaspergillus oryzae on xylan-based media.	aspergillus fumigatus anda. oryzae were cultivated in laboratory fermenters on media containing xylan as the main carbon source.a. fumigatus produced xylanase on unsubstituted, insoluble beech xylan but growth and enzyme production on soluble xylo-oligosaccharides from the steaming of hardwood were poor due to the presence of inhibitors. an essential prerequisite for good xylanase production bya. fumigatus was decrease in the ph of the cultivation below 3.0 at higher ph values, the production of ...	1993	24419846
longitudinal changes in skin-prick test reactivity.		1993	10779299
optimized preparation of aspergillus fumigatus extracts for allergy diagnosis.	extracts of aspergillus fumigatus are required for the measurement of specific antibodies that are important indices in the diagnosis of allergic bronchopulmonary aspergillosis (abpa). this study investigated the effect of different culture conditions on the production and release of antigenic and allergenic proteins of a. fumigatus. increasing the incubation temperature from 25 degrees c to 37 degrees c altered the production of proteins by the mycelium which resulted in the release of a greate ...	1993	10780890
metabolism of p-cresol by the fungus aspergillus fumigatus.	the fungus aspergillus fumigatus atcc 28282 was shown to grow on p-cresol as its sole source of carbon and energy. a pathway for metabolism of this compound was proposed. this has protocatechuate as the ring-fission substrate with cleavage and metabolism by an ortho-fission pathway. the protocatechuate was formed by two alternative routes, either by initial attack on the methyl group, which is oxidized to carboxyl, followed by ring-hydroxylation, or by ring-hydroxylation as the first step with s ...	1993	16348913
microbial transformations of steroids.		1993	8131902
diagnostic exercise: sudden death in colony-housed rufous hummingbirds (selasphorus rufus).		1993	8277734
[pulmonary mycetoma treatment in neutropenic patients].		1993	8281206
detection of galactomannan and the 18 kda antigen from aspergillus fumigatus in serum and urine from cattle with systemic aspergillosis.	serum and urine samples from cattle with experimental and spontaneous systemic mycotic infections were tested for the presence of galactomannan and the 18 kda antigen from aspergillus fumigatus by an inhibition elisa and immunoblotting, respectively. high levels of galactomannan (approximately 80 ng/ml.) were detected in serum from two of three calves experimentally infected with a. fumigatus. in two out of three cows with spontaneous acquired aspergillosis a similar amount of galactomannan was ...	1993	8284952
invasive aspergillosis of the temporal bone: an unusual manifestation of acquired immunodeficiency syndrome.		1993	8285317
efficacy of itraconazole solution in a rabbit model of invasive aspergillosis.	the efficacy of an itraconazole-cyclodextrin solution against aspergillus fumigatus was assessed in an immunosuppressed, temporarily leukopenic rabbit model of invasive aspergillosis and compared with that of amphotericin b. oral itraconazole solution at dosages of 20 and 40 mg/kg/day improved survival as compared with that of controls. itraconazole (40 mg/kg/day) not only improved survival and reduced antigen levels but also significantly eradicated a. fumigatus from tissues and was as effectiv ...	1993	8285610
in vitro and in vivo antifungal activities of d0870, a new triazole agent.	in vitro and in vivo antifungal activities of d0870 were evaluated in comparison with those of fluconazole. d0870, which is the r-enantiomer of ici195,739, was found to be the mycologically active enantiomer by comparing the activities of d0870 with those of m16355 (s-enantiomer of ici195,739). d0870 showed a broad spectrum of antifungal activity and mics and minimum antibiotic concentrations 4- to 2,000-fold lower in synthetic amino acid medium (fungal) agar than those of fluconazole for variou ...	1993	8285626
local and systemic antibody production in horses affected with chronic obstructive pulmonary disease.	an enzyme-linked immunosorbent assay (elisa) was used to quantify isotype-specific antibody to micropolyspora faeni and to aspergillus fumigatus in the sera and bronchoalveolar lavage fluid (balf) of normal horses, horses with chronic obstructive pulmonary disease (copd) and horses with other chronic respiratory diseases. elevated antibody levels were not detected in the sera of affected horses. however, both ige and iga antibody to both allergens was significantly elevated in balf in copd affec ...	1993	8291200
phosphotransferase system of streptococcus salivarius: characterization of the ptsh gene and its product.	the streptococcus salivarius ptsh gene encoding histidine-containing phosphocarrier protein (hpr) of the phosphotransferase system (pts) has been cloned, sequenced, and found to be part of a ptsh, ptsi operon. upstream from ptsh, putative -35 and -10 boxes and a shine-dalgarno sequence highly similar to the escherichia coli consensus regulatory elements were identified. a second promoter, located in the ptsh coding sequence was also observed and is sufficient for the expression of the s. salivar ...	1993	8294015
[semi-invasive pulmonary aspergillosis. apropos of a case].	a 43-year-old man was admitted to hospital for excavated pneumopathy of the upper part of the right lung, revealed by signs of respiratory infection evolving for a few weeks and involving a 10-kg weight loss. the radiological and clinical evolution and the demonstration of aspergillus fumigatus allowed establishing a diagnosis of semi-invasive aspergillosis. the treatment, associating amphotericin b and 5-fluorocytosine, replaced by itraconazole 15 days later, soon produced clinical healing and ...	1993	8296152
cell wall antigens in aspergillus fumigatus.	the aspergillus cell wall contains most of the antigens secreted by the fungus during its active in vitro or in vivo growth. these antigens, which bind to the ige and igg of allergic and aspergilloma patients or are secreted in the biological fluids of patients with invasive aspergillosis, are of primary importance in the diagnosis of aspergillosis. located at the interface between host and pathogen cells, the fungal cell wall plays a major role during fungal invasion. it contains several surfac ...	1993	8298277
comparative efficacy of amphotericin b, clotrimazole and itraconazole against aspergillus spp. an in vitro study.	the susceptibilities of two isolates of aspergillus flavus, one from a human case of recalcitrant mycotic keratitis, and an environmental isolate of a. fumigatus, to itraconazole, clotrimazole and amphotericin b were measured. observations of macroscopic growth and microscopic evaluations of conidia germination both indicated that the two isolates of a. flavus were markedly more resistant to amphotericin b than to itraconazole and clotrimazole. itraconazole was more effective than clotrimazole f ...	1993	8302362
[value of tc99m-nanocolloid spect in diagnosis and follow-up of necrotizing external otitis].	necrotizing external otitis (neo), or malignant external otitis, a severe penetrating infection of the ear canal and the temporal bone, which occurs almost exclusively in diabetic or immunodepressed elderly patients, is caused in most cases by pseudomonas aeruginosa while staphylococcus epidermidis and aspergillus fumigatus have been described as other possible agents. recently bone scintigraphy, carried out above all employing spect, has been considered an important tool in diagnosis and follow ...	1993	8209690
serum igg antibodies to mold spores in two norwegian sawmill populations: relationship to respiratory and other work-related symptoms.	wood trimmers and planing operators from two separate sawmill populations (n = 303 and 170) were studied by serology assessment and a self-administered questionnaire. igg antibodies to rhizopus microsporus ssp. rhizopodiformis, paecilomyces variotii, and aspergillus fumigatus were measured by elisa. the questionnaire included questions about general respiratory symptoms and symptoms after handling moldy timber. personal exposure of wood trimmers to mold spores and wood dust was measured in one p ...	1993	8213847
a proposed pathogenetic process in the formation of aspergillus mycotic aneurysm in the central nervous system.	by selectively infiltrating and destroying the internal elastica of a major cerebral artery, aspergillus fungus (af) induces disruption and incipient dilatation of the vascular wall with or without inflammation. this unique pathogenetic mechanism of forming "true" fungal mycotic aneurysms (fmas) was clearly demonstrated in a middle-aged adult male who died of a pontine stroke. the latter was secondary to thrombosis in the basilar artery of which the internal elastica was infiltrated and replaced ...	1993	8215210
intracranial aspergillus granuloma.	intracranial aspergillus granulomas over a 12-year period were studied retrospectively. three distinctive types of presentations were seen, namely nine patients had the rhinocerebral form with primary involvement of the air sinuses and secondary involvement of the skull base, cranial nerves and brain, three patients with the apparently purely intracranial form presented like any other space occupying lesion and one patient who had a 'stroke-like' presentation had a parasellar lesion. none of the ...	1993	8216908
heat resistance of fungi from soil.	the survival of fungi from soil samples has been investigated after exposure to temperatures of 60, 70, 80 and 90 degrees c in sabouraud agar. the least resistant fungi surviving 60 degrees c for 60 min were the aspergillus (a.) niger group, chaetomium spp, penicillium spp, and scytalidium lignicola. none of these survived 70 degrees c for 10 min. the next group surviving 70 degrees c for 60 min included the a. glaucus group, byssochlamys nivea, dichotomomyces cejpii, gelasinospora spp, rhizocto ...	1993	8217516