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modelling lactation curve for milk fat to protein ratio in iranian buffaloes (bubalus bubalis) using non-linear mixed models.the aim of this study was to compare seven non-linear mathematical models (brody, wood, dhanoa, sikka, nelder, rook and dijkstra) to examine their efficiency in describing the lactation curves for milk fat to protein ratio (fpr) in iranian buffaloes. data were 43 818 test-day records for fpr from the first three lactations of iranian buffaloes which were collected on 523 dairy herds in the period from 1996 to 2012 by the animal breeding center of iran. each model was fitted to monthly fpr record ...201627600968
separation and quantification of milk casein from different buffalo breeds.understanding the milk protein expression profile in different buffalo breeds plays an important role in improving hybrid selection and determining the effects on milk protein synthesis. the aim of this research is to compare the differences in milk protein content, composition and distribution between river buffalo and their crossbreeds for hybrid screening. four groups of milk samples that included nili-ravi (n), murrah (m), a nili-ravi-murrah crossbreed (m-n), and a crossbreed of river buffal ...201627600966
relationship between the ovarian follicular response at the start of an ovsynch-tai program and pregnancy outcome in the mediterranean river buffalo.the aim of this study was to evaluate the relationship between the ovarian follicular response at the start of the ovsynch-fixed-time artificial insemination (tai) program and pregnancy outcome in the mediterranean river buffalo. the study involved 116 pluriparous buffaloes at 118 ± 65 days in milk maintained under commercial conditions on a buffalo dairy enterprise in southern italy. synchronization of the stage of the estrous cycle in the ovsynch-tai program involves an injection of gnrh on da ...201627577986
cryoprotection synergism between glycerol and dimethyl sulfoxide improves the mitochondrial transmembrane potential, plasmalemma, acrosomal and dna integrities, and in vivo fertility of water buffalo (bubalus bubalis) spermatozoa.the objective of the study was to devise a cryoprotection synergism between glycerol and dimethyl sulfoxide (dmso) for water buffalo spermatozoa. additionally, the effect of best evolved concentrations of glycerol and dmso in extender was assessed on in vivo fertility of buffalo spermatozoa. ejaculates (n = 30) were equally distributed into five aliquots; first aliquot was diluted at 37 °c in extender having 7 % glycerol (control); second aliquot was diluted at 37 °c as well as at 4 °c in extend ...201627761771
effect of growth differentiation factor-9 (gdf-9) on the progression of buffalo follicles in vitrified-warmed ovarian tissues.to improve the reproductive performance of water buffalo to level can satisfy our needs, the mechanisms controlling ovarian follicular growth and development should be thoroughly investigated. therefore, in this study, the expressions of growth differentiation factor-9 (gdf-9) in buffalo ovaries were examined by immunohistochemistry, and the effects of gdf-9 treatment on follicle progression were investigated using a buffalo ovary organ culture system. frozen-thawed buffalo ovarian follicles wit ...201627554536
effect of melatonin on maturation capacity and fertilization of nili-ravi buffalo (bubalus bubalis) oocytes.this study evaluated the effect of melatonin supplementation of in vitro maturation media on in vitro maturation (ivm) and in vitro fertilization (ivf) rate of buffalo oocytes. cumulus oocytes complexes (cocs) were aspirated from follicles of 2-8 mm diameter. in experiment i, cocs were matured in ivm medium supplemented with 0 (control), 250, 500, and 1000 μm melatonin for 22-24 hours in co2 incubator at 38.5°c with 5% co2 and at 95% relative humidity. the maturation rate did not differ in media ...201627540514
differences in developmental competence and gene expression profiles between buffalo (bubalus bubalis) preimplantation embryos cultured in three different embryo culture media.the objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. after ivm/ivf presumptive zygotes were cultured in one of three systems: undefined tcm-199, mcr2aa medium supplemented with 10 % fbs and defined pva-myo-inositol-phosphate-egf medium. no (p > 0.05) differences at 2-cell, 4-cell and 8-cell to 16- cell stages were observed among the three cultured medi ...201627481470
genetic association between snps in the dgat1 gene and milk production traits in murrah buffaloes.this study identified polymorphisms in the dgat1 gene in murrah buffaloes and investigated the associations to milk production and quality traits (milk, fat and protein yields and percentages, somatic cell count). genomic dna was extracted from hair follicles collected from the tail of 196 females. three snps were identified in dgat1 gene by sequencing. statistical analyses were performed to verify the linkage and the association between polymorphisms and traits. the estimated value of r (2) bet ...201627469895
genetic diversity analysis of buffalo fatty acid synthase (fasn) gene and its differential expression among bovines.fatty acid synthase (fasn) gene seems to be structurally and functionally different in bovines in view of their distinctive fatty acid synthesis process. structural variation and differential expression of fasn gene is reported in buffalo (bubalus bubalis), a bovine species close to cattle, in this study. amino acid sequence and phylogenetic analysis of functionally important thioesterase (te) domain of fasn revealed its conserved nature across mammals. amino acid residues at te domain, responsi ...201626376068
magnetic resonance imaging and computer tomography of brain lesions in water buffaloes and cattle stunned with handguns or captive bolts.owing to the demand for genuine mozzarella, some 330 water buffaloes are being slaughtered every year in switzerland albeit a stunning procedure meeting animal welfare and occupational safety requirements remains to be established. to provide a basis for improvements, we sized anatomical specifics in water buffaloes and cattle and we assessed brain lesions after stunning with captive bolts or handguns by diagnostic imaging. in water buffaloes and cattle, the median distance from the frontal skin ...201626610289
loss of heat shock protein 70 from apical region of buffalo (bubalus bubalis) sperm head after freezing and thawing.the post-thaw fertility of frozen-thawed mammalian spermatozoa is substantially low as compared with that of fresh sperm. furthermore, the post-thaw fertility of the cryopreserved buffalo sperm has been reported to be poor as compared with that of cattle sperm. recently, heat shock protein 70 (hsp70) has been found to play a critical role in mammalian fertilization and early embryonic development in boar and cattle. however, the presence of such fertility-related hsp70 in buffalo sperm and its s ...201626607876
profile of muscle tissue gene expression specific to water buffalo: comparison with domestic cattle by genome array.in contrast with the past, the water buffalo is now not only a draft animal, but also an important food source of milk and meat. it is increasingly apparent that the water buffalo have huge potential for meat production, but its breeding needs to be investigated. regarding the molecular mechanisms involved in the meat quality difference between the buffalo (bubalus bulabis) and yellow cattle (bos taurus), 12 chemical-physical characteristics related to the meat quality of longissimus thoracis mu ...201626598327
molecular epidemiology of pathogenic leptospira spp. among large ruminants in the philippines.the extent of leptospira infection in large ruminants resulting to economic problems in livestock industry in a leptospirosis-endemic country like the philippines has not been extensively explored. therefore, we determined the prevalence and carrier status of leptospirosis in large ruminants using molecular techniques and assessed the risk factors of acquiring leptospirosis in these animals. water buffalo and cattle urine samples (n=831) collected from 21 farms during 2013-2015 were subjected to ...201627452879
a novel selection signature in stearoyl-coenzyme a desaturase (scd) gene for enhanced milk fat content in bubalus bubalis.modern molecular interventions are dynamic gears for breeding animals with superior genetic make-up. these scientific efforts lead us toward sustainable dairy herds with improved milk production in terms of yield and quality. many of candidate genes have been dissected at molecular level, and suitable genetic markers have been identified in cattle, but this work has not been validated in buffaloes so far. stearoyl-coenzyme a desaturase (scd) has been a potential candidate gene for fat content of ...201627307277
simultaneously tracing the geographical origin and presence of bovine milk in italian water buffalo mozzarella cheese using maldi-tof data of casein signature peptides.water buffalo (wb) casein (cn) and curd samples from indigenous italian and international breeds were examined with the objective of identifying signature peptides that could function as an indicator to determine the origin of their milk products. cn in complex mixtures were digested with trypsin, and peptide fragments were subsequently identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tof ms). the unique presence of a β-cn a variant and an interna ...201627299776
assessment of foot health and animal welfare: clinical findings in 229 dairy mediterranean buffaloes (bubalus bubalis) affected by foot disorders.lameness represents the third most important health-related cause of economic loss in the dairy industry after fertility and mastitis. although, dairy mediterranean buffaloes (mb) and dairy cows share similar breeding systems predisposing to similar herd problems, published studies exploring its relevance and role in these ruminants are still rare and incomplete. the aims of this study were to describe the clinical findings of foot disorders (fds) in dairy mb and their influence on animal welfar ...201627297174
sister chromatid exchange test in river buffalo lymphocytes treated in vitro with furocoumarin extracts.furocoumarin extracts from psoralea morisiana, the endemic sardinian legume species, were tested for their mutagenic potential on river buffalo blood cells. the results obtained performing the sister chromatid exchange (sce) test in blood cultures of five river buffalo calves (exposure to furocoumarins for 72h) and five cows (exposure to furocoumarins for 3h, in the absence and presence of s9 metabolic activator) are reported. significant differences in mean values of sces were observed in cells ...201627180332
effect of mastitis on luteal function and pregnancy rates in buffaloes.the aim of this study was to investigate the effects of mastitis on cl development and function and pregnancy rate in buffaloes. sixty-six buffaloes (bubalus bubalus) reared in a commercial farm at el-beheira governorate, north of egypt were used in this study. according to the visual observation of milk, physical examination of the udder and actual somatic cell count in milk, buffalo cows were divided into three groups: without mastitis (w), n = 23; subclinical mastitis (sc), n = 18; and clinic ...201627177967
high genetic diversity and distribution of bubu-dqa alleles in swamp buffaloes (bubalus bubalis carabanesis): identification of new bubu-dqa loci and haplotypes.in this study, genetic diversity analysis of mhc class ii-dqa locus helped in identification of 25 new bubu-dqa nucleotide sequences in swamp buffaloes (bubalus bubalis carabanesis, bubu). phylogenetic analysis revealed the distribution of the buffalo dqa sequences in two major clusters of dqa1 and dqa2 genes, sharing common lineages with corresponding cattle alleles, possibly due to trans-species evolution. however, a highly divergent sequence, bubu-dqa*2501, homologous to cattle (bola) dqa3 al ...201627177904
polymorphism in the a2m gene associated with high-quality milk in murrah buffaloes (bubalus bubalis).the study of genes associated with host defense mechanisms, such as the a2m gene, plays a critical role in preventing diseases that reduce milk yield and its constituents. the aim of this study was to identify polymorphisms in the a2m gene in murrah buffaloes (bubalus bubalis), and investigate their associations with milk yield, fat and protein production, fat and protein percentages, and somatic cell count. hair follicle samples of 136 animals were collected for dna extraction, and polymorphism ...201627173184
expression of putative stem cell marker, hepatocyte nuclear factor 4 alpha, in mammary gland of water buffalo.buffaloes account for more than 56% of total milk production in india. cyclic remodeling of mammary glands of human, mice, cow, sheep, and goat is determined by mammary stem cells. it is logical to assume that buffalo mammary gland will have mammary stem/progenitor cells. thus far, no report exists on identification of buffalo mammary stem cells. hepatocyte nuclear factor 4 alpha (hnf4a) is a candidate marker for hepatic progenitor cells and has recently been suggested as a marker of bovine mamm ...201627092988
how livestock and flooding mediate the ecological integrity of working forests in amazon river floodplains.the contribution of working forests to tropical conservation and development depends upon the maintenance of ecological integrity under ongoing land use. assessment of ecological integrity requires an understanding of the structure, composition, and function and major drivers that govern their variability. working forests in tropical river floodplains provide many goods and services, yet the data on the ecological processes that sustain these services is scant. in flooded forests of riverside am ...201627039519
isolation and identification of prepubertal buffalo (bubalus bubalis) spermatogonial stem cells.isolation and culture of spermatogonial stem cells (sscs) are attractive for production of genetic modified offspring. in the present study, buffalo spermatogonial stem-like cells were isolated, cultured and expression pattern of different germ cell marker genes were determined. to recover spermatogonia, testes from age 3 to 7 months of buffalo were decapsulated, and seminiferous tubules were enzymatically dissociated. two types of cells, immature sertoli cell and type a spermatogonia were obser ...201626954139
sequence analysis of the s1pr1 gene in river buffalo.recent developments in methodologies for genomic analyses have enabled a significant advance in understanding of the river buffalo genome. the s1pr1 gene has been mapped to buffalo chromosome 6 and bovine chromosome 3; this gene is of interest as it is a candidate for marbling in meat, an important economic trait. here, we performed next generation sequencing in a buffalo bac dna clone and obtained a 54.5-kb sequence encompassing the entire buffalo s1pr1 gene as well as the 27 kb upstream region ...201626909928
investigation into omocysteine, vitamin e and malondialdehyde as indicators of successful artificial insemination in synchronized buffalo cows (bubalus bubalis).the aim of this study was to describe modifications in plasma homocysteine (hcy), vitamin e (vite) and malondialdehyde (mda) concentrations in the first 56 days after artificial insemination (ai) in buffalo. thirty-five buffalo cows were divided, ex post, into three groups on the basis of pregnancy diagnosis: pregnant, not pregnant, with embryonic mortality. pregnancy was diagnosed by ultrasonography and plasma concentrations of pregnancy-associated glycoproteins (pags). our results showed that, ...201626850546
rapid detection of water buffalo ricotta adulteration or contamination by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.the improvement and development of novel technologies and analytical tools are an important first line of defence for both detecting and deterring food frauds. in order to protect the authenticity of traditional foods and safeguard geographical indications and traditional specialities at european level, protected designation of origin (pdo) legislation has been introduced as a guarantee of quality (council regulation ecc 2081/92). dairy products, especially those certified as pdo products, are a ...201626777680
208 production of transgenic cloned buffalo (bubalus bubalis) embryos containing human insulin gene through hand-guided cloning.diabetes is a growing disease worldwide and has emerged as a major healthcare problem in india. insulin is an essential medicine for the treatment of diabetes. large dairy animals, such as buffaloes and cows, may be used as bioreactors for cost-effective production of human insulin. the present study was aimed to produce transgenic buffalo embryos containing the human insulin gene through hand-guided cloning for production of transgenic animals. buffalo female fetal fibroblast cells at passage n ...201628279005
164 carnitine improves post-thawing sperm motility by increasing adenosine triphosphate content in buffalo (bubalus bubalis).semen cryopreservation plays a critical role for a wide application of both ai and in vitro embryo production in buffalo. in this species, spermatozoa are more susceptible to hazards during freezing and thawing than cattle spermatozoa, thus resulting in lower fertilizing potential (andrabi et al. 2008 anim. reprod. sci. 104, 427-433). carnitine is a quaternary ammonium compound with antioxidant capacities, able to reduce the availability of lipids for peroxidation by transporting fatty acids int ...201628278961
151 effect of ascorbic acid on oxidative stress and its thermoprotectant role on in vitro embryonic development of buffalo (bubalus bubalis) embryos.the most important factors that lead to stress in farm animals are oxidative and thermal stress, leading to reduced reproductive efficiency. oxidative stress leads to an increase in proportion of reactive oxygen species, whereas heat stress affects the physiology of animals, which lowers the conception rates of dairy cattle. in vitro culture systems have been enhanced by manipulating media with various supplements such as vitamins, growth factors, and antioxidants that have overcome these proble ...201628278948
33 buffalo (bubalus bubalis) embryos produced by hand-made cloning and in vitro fertilization differ in their global transcriptome profile.although the blastocyst rate obtained with nuclear transferred (nt) embryos is higher than that obtained following in vitro fertilization (ivf) in buffalo, the live birth rate of nt embryos is <2% across different farm animal species compared with a birth rate >40% obtained with ivf embryos. this is believed to be due primarily to incomplete or incorrect nuclear reprogramming of the donor somatic cell by the oocyte, which results in aberrant embryonic gene expression. we compared the global tran ...201628278830
32 next-generation sequencing discloses differences in microrna expression profiles of buffalo (bubalus bubalis) embryos produced by hand-made cloning and in vitro fertilization.mammalian embryo development is a complex process with a series of critical events taking place at every stage of development. it is an established fact that the birth rate of animals produced by nuclear transfer (nt) is far less (<2%) than that of ivf embryos (40%) after successful embryo transfers in different farm animal species. micro(mi)rnas are small non-coding rnas of 17 to 25 nucleotides that alter the function of their target genes by either degrading them or inhibiting their expression ...201628278829
strong and stable geographic differentiation of swamp buffalo maternal and paternal lineages indicates domestication in the china/indochina border region.the swamp type of the asian water buffalo is assumed to have been domesticated by about 4000 years bp, following the introduction of rice cultivation. previous localizations of the domestication site were based on mitochondrial dna (mtdna) variation within china, accounting only for the maternal lineage. we carried out a comprehensive sampling of china, taiwan, vietnam, laos, thailand, nepal and bangladesh and sequenced the mtdna cytochrome b gene and control region and the y-chromosomal zfy, sr ...201626677084
mass spectrometry detection of fraudulent use of cow whey in water buffalo, sheep, or goat italian ricotta cheese.ricotta cheese is a typical italian product, made with whey from various species, including cow, buffalo, sheep, and goat. ricotta cheese nominally manufactured from the last three species may be fraudulently produced using the comparatively cheaper cow whey. exposing such food frauds requires a reliable analytical method. despite the extensive similarities shared by whey proteins of the four species, a mass spectrometry-based analytical method was developed that exploits three species-specific ...201626675863
molecular characterization of buffalo haptoglobin: sequence based structural comparison indicates convergent evolution between ruminants and human.haptoglobin (hp) protein has high affinity for hemoglobin (hb) binding during intravascular hemolysis and scavenges the hemoglobin induced free radicals. earlier reports indicate about uniqueness of hp molecule in human and cattle, but in other animals, it is not much studied. in this paper, we characterized buffalo hp molecule and determined its molecular structure, evolutionary importance, and tissue expression. comparative analysis and predicted domain structure indicated that the buffalo hp ...201626646629
molecular identification and seasonal infections of species of fasciola in ruminants from two provinces in china.we determined the prevalence and seasonality of infections by fasciola of goats and bovine species (cattle and water buffalo) in hubei and anhui provinces of china. faecal samples were collected at 2- to 3-month intervals from 200 goats in hubei province and from 152 bovine species in anhui province. all faecal samples were examined for the presence of parasites. we determined the nucleotide sequences of the first and second internal transcribed spacers (its-1 and its-2) of the nuclear ribosomal ...201626123576
the resurrection of a species: sarcocystis bovifelis heydorn et al., 1975 is distinct from the current sarcocystis hirsuta in cattle and morphologically indistinguishable from sarcocystis sinensis in water buffaloes.in the mid-1970s, it was established through transmission experiments and ultrastructural studies of sarcocysts by transmission electron microscopy (tem) that cattle was the intermediate host of three sarcocystis spp. using dogs, cats and humans, respectively, as definitive hosts. the cat-transmitted species with microscopic sarcocysts was initially named sarcocystis bovifelis, but it was soon renamed sarcocystis hirsuta, since it was considered to be identical with a previously named species. i ...201626462803
prevalence and first molecular identification of sarcocystis species in cattle and water buffaloes in india.the importance of sarcocystis hominis in causing zoonotic infections is well known. recently, s. hominis like cysts have been reported from water buffalo in china. previous studies indicate prevalence of sarcocystis species in bovine populations in india but molecular evidence is required for proper species differentiation. we examined two hundred and ninety six cardiac tissue samples of indian water buffaloes and cattle from northern and western parts of the country. tissues were examined for s ...201627447215
sarcocystis rommeli, n. sp. (apicomplexa: sarcocystidae) from cattle (bos taurus) and its differentiation from sarcocystis hominis.cattle (bos taurus) are intermediate hosts for three named species of sarcocystis, s. cruzi, s. hirsuta, and s. hominis. recently, a fourth species was identified and named s. sinensis. however, s. sinensis originally named a species of sarcocystis in water buffalo (bubalus bubalis) in china. based on unverifiable evidence, it was suggested that the same parasite infects cattle. in addition, s. sinensis was recently declared as nomen nudum because its naming violated the rules of international c ...201626111603
changes in the physico-chemical and microbial quality during the production of pastırma cured with different levels of sodium nitrite.pastırma, a dry-cured meat product, is produced from the whole muscle and/or muscles obtained from certain parts of beef and water buffalo carcasses. the purpose of this study was to determine the effects of different levels of sodium nitrite on changes in the physicochemical and microbial quality during the production of pastırma. the changes in residual nitrite, salt, ph, moisture, thiobarbutiric acid reactive substances (tbars), colour (l*, a*, b*), total aerobic mesophilic bacteria (tamb), l ...201627857537
metabolomics of the antipyretic effects of bubali cornu (water buffalo horn) in rats.bubali cornu (water buffalo horn, wbh) has been used for thousands of years in traditional chinese medicine (tcm) as an effective treatment for heat. in the present study, we have carried out a metabolomics profiling study on plasma and urine samples to explore potential biomarkers and determine how wbh exerts its antipyretic effects in yeast-induced pyrexia at a metabolomic level. ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (uplc- ...201627384078
complete genome sequence of a lytic siphoviridae bacteriophage infecting several serovars of salmonella enterica.the bacteriophage 100268_sal2 was isolated from water buffalo feces in southern italy, exhibiting lytic activity against several subspecies of salmonella enterica this bacteriophage belongs to the siphoviridae family and has a 125,114-bp double-stranded dna (ds-dna) genome containing 188 coding sequences (cdss).201627688334
complete genome sequences of three siphoviridae bacteriophages infecting salmonella enterica serovar enteritidis.three bacteriophages, 118970_sal1, 118970_sal2, and 64795_sal3, were isolated from water buffalo feces in southern italy, exhibiting lytic activity against salmonella enterica serovar enteritidis. these bacteriophages belong to the siphoviridae family and have a 60,113-bp, 123,930-bp, and 48,094-bp double-stranded dna (dsdna) genome containing 72, 173, and 80 coding sequences (cdss), respectively.201627856572
molecular differentiation of sarcocystis buffalonis and sarcocystis levinei in water buffaloes (bubalus bubalis) from sarcocystis hirsuta and sarcocystis cruzi in cattle (bos taurus).the purpose of the present study was to obtain sarcocysts of sarcocystis buffalonis and sarcocystis levinei from water buffaloes and characterize the isolates by molecular methods in order to determine whether the two species were genetically different from sarcocystis hirsuta and sarcocystis cruzi, respectively, from cattle, which had been characterized before. about 35 macroscopically visible (3-4 × 1-2 mm) and 20 barely visible (1-3 × 0.2 mm) sarcocysts were excised from the esophagus of 18 n ...201626979729
molecular characterisation of sarcocystis bovifelis, sarcocystis bovini n. sp., sarcocystis hirsuta and sarcocystis cruzi from cattle (bos taurus) and sarcocystis sinensis from water buffaloes (bubalus bubalis).about 200 individual sarcocysts were excised from 12 samples of cattle beef from five countries (argentina, brazil, germany, new zealand, uruguay) and tentatively identified to species or cyst type on the basis of their size and shape and cyst wall morphology. genomic dna was extracted from 147 of these sarcocysts and used initially for pcr amplification and sequencing of the partial mitochondrial cytochrome c oxidase subunit i gene (cox1) in order to identify the sarcocysts to species and/or se ...201626677095
combined il-12 plasmid and recombinant sjgst enhance the protective and anti-pathology effect of sjgst dna vaccine against schistosoma japonicum.schistosomiasis is listed as one of most important tropical diseases and more than 200 million people are estimated to be infected. development of a vaccine is thought to be the most effective way to control this disease. recombinant 26-kda glutathione s-transferase (rsjgst) has previously been reported to achieve a worm reduction rate of 42-44%. to improve the efficiency of the vaccine against schistosoma japonicum, we immunized mice with a combination of pcdna vector-encoded 26-kda sjgst (pcdn ...201626891172
domestic cats (felis catus) are definitive hosts for sarcocystis sinensis from water buffaloes (bubalus bubalis).the definitive hosts of sarcocystis sinensis in water buffaloes have hitherto been unknown, but the close similarity of this species to the cat-transmitted sarcocystis bovifelis in cattle suggested they were felids. in a previous study, two domestic cats were fed macroscopic sarcocysts of sarcocystis fusiformis contained within or dissected from the esophageal muscles of water buffaloes, while no microscopic sarcocysts of s. sinensis were noticed. both cats started shedding small numbers of spor ...201627075117
trypanosoma vivax in water buffalo of the venezuelan llanos: an unusual outbreak of wasting disease in an endemic area of typically asymptomatic infections.trypanosoma vivax has been associated with asymptomatic infections in african and south american buffalo. in this study, t. vivax was analyzed in water buffalo (bubalus bubalis) from venezuela in a molecular survey involving 293 blood samples collected from 2006 to 2015 across the llanos region. results demonstrated constant infections (average 23%) during the years analyzed. in general, animals were healthy carriers of t. vivax with low levels of parasitemia and were diagnosed exclusively by tv ...201627884441
short communication: role of streptococcus pluranimalium in mediterranean buffaloes (bubalus bubalis) with different udder health statuses.the aims of the current study were to describe presence and clinical role over time of streptococcus pluranimalium isolated in milk samples of mediterranean buffalo (mb). two hundred composite milk samples originating from 40 primiparous mb were collected at 10, 30, 60, 90, and 150d in milk (dim) and from 20 pluriparous mb at 77 to 120 dim. milk samples were used for analysis of somatic cell counts, bacteriological cultures, and identification (matrix-assisted laser desorption/ionization time-of ...201626805969
no change in mrna expression of immune-related genes in peripheral blood mononuclear cells challenged with theileria annulata in murrah buffalo (bubalus bubalis).water buffaloes (bubalus bubalis) act as carrier to theileria annulata and show less clinical sign of tropical theileriosis as compared to indigenous and exotic cattle. differential expression of immune-related genes such as major histocompatibility complex, class ii, dq alpha 1 (mhc-dqα), signal-regulatory protein alpha (sirpa), prion protein (prnp), toll-like receptor 10 (tlr10), c-musculoaponeurotic fibrosarcoma oncogene homolog (cmaf) and v-maf avian musculoaponeurotic fibrosarcoma oncogene ...201626997138
fatal theileria orientalis n2 genotype infection among asian water buffaloes (bubalus bubalis) in a commercial dairy farm in kerala, india.fifteen dairy buffaloes of a farm in the state of kerala, india developed fatal oriental theileriosis within 2 months of their procurement. typical piroplasms of theileria orientalis were observed in the erythrocytes of all affected animals by giemsa-leishman staining of blood smears. case fatality rate was 87·5% (seven out of eight) in the clinically progressed cases. therapeutic management with anti-theilerial drugs buparvaquone and oxytetracycline led to recovery of seven other animals in les ...201626522773
molecular cloning, polymorphism, and functional activity of the bovine and water buffalo mx2 gene promoter region.bovine mx2 gene sequences were already reported, but further information about the gene properties is not yet available. the objective of the current study was to elucidate the structural properties of the bovine mx2 gene mainly the promoter region and its possible functional role. if available, such information would help in assessing the functional properties of the gene, which was reported to confer antiviral action against recombinant vsv.201628066698
exploration of luteinizing hormone in murrah buffalo (bubalus bubalis) urine: extended surge window opens door for estrus prediction.estrus detection in buffaloes has been a major concern for decades, and lack of reliable methods affects their effective reproductive management. luteinizing hormone (lh) detection in urine is in practice for several mammals for timed insemination, whereas very few reports are available on buffalo urinary lh. the focus of this study is to detect the presence of lh in buffalo urine, quantitate variation in urinary lh during different estrous cycle phases and examine the duration of mid-cycle lh w ...201628011259
protein modeling and molecular dynamics simulation of cloned regucalcin (rgn) gene from bubalus bubalis.regucalcin (rgn), a calcium regulating protein having anti prolific, anti-apoptotic functions, plays important part in the biosynthesis of ascorbic acid. it is a highly conserved protein that has been reported from many tissue types of various vertebrate species. employing its effect of regulating enzyme activities through reaction with sulfhydryl group (-sh) and calcium, structural level study is believed to offer a better understanding of its binding properties along with information exploited ...201628000561
prevalence of antibodies against bubaline herpesvirus (buhv-1) among mediterranean water buffalo (bubalus bubalis) with implications in buffalo trade.both bovine herpesvirus (bohv-1) and bubaline herpesvirus (buhv-1) have been reported to cross the species barrier. antibody seroconversion in glycoprotein e (ge) blocking elisa during buhv-1 infection has been documented. recent diagnostic efforts have focused on the development and application of discriminatory tests to distinguish between infections with bohv-1 and buhv-1.201627334411
antibodies to bovine viral diarrhoea virus (bvdv) in water buffalo (bubalus bubalis) and cattle from the northern territory of australia.farmed and feral water buffaloes (bubalus bubalis) populations often coexist with cattle in the northern territory of australia, but their level of exposure to bovine viral diarrhoea virus (bvdv) is unknown.201627785794
replacement of serum with ocular fluid for cryopreservation of immature testes.cryopreservation of immature testis is a feasible approach for germplasm preservation of male animals. combinations of dimethyl sulfoxide (dmso) and foetal bovine serum (fbs) are used for testis cryopreservation. however, an alternative to fbs is needed, because fbs is expensive. buffalo ocular fluid (buof), a slaughter house by-product, could be an economical option. the objective of the present study was to assess whether buof can replace fbs for cryopreservation of immature mouse (mus musculu ...201627693391
clinical, laboratory and ultrasonographic findings in egyptian buffalo (bubalus bubalis) with caecal and colonic dilatation.the present study was conducted to describe the clinical, laboratory and ultrasonographic findings of caecal and colonic dilatation in egyptian buffalo (bubalus bubalis). a total number of forty buffaloes were included in the study and divided into two groups: control group (n = 20) and diseased group (n = 20). diseased buffalo were admitted to the veterinary teaching hospital at assiut university-egypt. each of the diseased animals was subjected to clinical, rectal, laboratory and ultrasonograp ...201630255032
role of linker histone h1c during the reprogramming of chinese swamp buffalo (bubalus bubalis) embryos produced by somatic cell nuclear transfer.during reprogramming, there is exchange of histone h1c and the oocyte-specific linker histone, and h1c may play a critically important role in the reprogramming process of somatic cell nuclear transfer (scnt). the aim of the present study was to investigate the role of the h1c gene in scnt reprogramming in chinese swamp buffalo (bubalus bubalis) using rna interference (rnai). chinese swamp buffalo h1c gene sequences were obtained and h1c-rnai vectors were designed, synthesised and then transfect ...201625145348
buffalo (bubalus bubalis) scnt embryos produced from somatic cells isolated from frozen-thawed semen: effect of trichostatin a on the in vitro and in vivo developmental potential, quality and epigenetic status.this study examined the effects of trichostatin a (tsa) treatment of reconstructed buffalo embryos, produced by hand-made cloning using somatic cells isolated from over a decade old frozen-thawed semen, on their in vitro and in vivo developmental competence, quality and epigenetic status. following treatment of reconstructed embryos with tsa (0, 50 or 75 nm) for 10 h prior to culture, the cleavage (100.0 ± 0, 94.5 ± 2.3 and 96.1 ± 1.2%, respectively) and blastocyst rate (50.6 ± 2.3, 48.4 ± 2.7 a ...201626503476
optixcell improves the postthaw quality and fertility of buffalo bull sperm.the present study was conducted to compare the liposome-containing, animal protein-free, commercially available optixcell extender with the tris-citric-egg yolk extender for postthaw quality and fertility of buffalo semen. semen was collected from five adult nili-ravi buffalo (bubalus bubalis) bulls of similar age group with an artificial vagina (at 42 °c) for 3 weeks (replicates). semen ejaculates from each buffalo bull were divided into two aliquots and diluted (at 37 °c having 50 × 10(6) sper ...201626522702
l-cysteine improves antioxidant enzyme activity, post-thaw quality and fertility of nili-ravi buffalo (bubalus bubalis) bull spermatozoa.the effects of l-cysteine in extender on antioxidant enzymes profile during cryopreservation, post-thaw quality parameters and in vivo fertility of nili-ravi buffalo bull spermatozoa were studied. semen samples from 4 buffalo bulls were diluted in tris-citric acid-based extender having different concentrations of l-cysteine (0.0, 0.5, 1.0, 2.0 and 3.0 mm) and frozen in 0.5-ml french straws. the antioxidative enzymes [catalase, super oxide dismutase and total glutathione (peroxidase and reductase ...201626762772
first report of cryptosporidium species in farmed and wild buffalo from the northern territory, australia.a molecular epidemiological survey of cryptosporidium from water buffalo (bubalus bubalis) in the northern territory in australia was conducted. fecal samples were collected from adult farmed (n = 50) and wild buffalo (n = 50) and screened using an 18s quantitative pcr (qpcr). positives were typed by sequence analysis of 18s nested pcr products. the qpcr prevalence of cryptosporidium species in farmed and wild buffalo was 30 and 12 %, respectively. sequence analysis identified two species: c. pa ...201626758449
209 production of buffalo (bubalus bubalis) embryos containing human lysozyme gene.lysozyme is a ubiquitous enzyme found in all major taxa of living organisms with a diverse role in human and animal health. it plays an important role as a nonspecific immune factor and anti-inflammatory factor and is a part of the innate immune system. research on the expression of recombinant human lysozyme is, thus, potentially valuable to the dairy industry. therefore, the present study was carried out to observe the developmental competence and quality of cloned embryos containing the human ...201628279006
198 successful transplantation of buffalo (bubalus bubalis) germ cells to homologous recipients.transplantation of isolated germ cells from a fertile donor male into the seminiferous tubules of infertile recipients can result in donor germ cells-derived sperm production. this technique has the potential to be used as an alternative strategy for producing transgenic livestock with higher efficiency and less time and capital requirement than the current methods. the objective of the present study was to investigate whether the homologous transplantation of isolated buffalo germ cells could r ...201628278995
121 three-dimensional assessment of early corpus luteum vascularity in buffalo (bubalus bubalis).the aim of the study was to develop an objective method to assess the vascular flow to the early corpus luteum (cl) in buffaloes using colour doppler ultrasound data. our hypothesis was that 3-dimensional (3d) volumetric analysis of vascularity would demonstrate lower variability between animals compared with conventional 2-dimensional (2d) analysis of single images. wave emergence and ovulation was synchronized in buffalo (n=16) using prostaglandin-gnrh based protocols. colour doppler ultrasono ...201628278918
the effect of a high-roughage diet on the metabolism of aromatic compounds by rumen microbes: a metagenomic study using mehsani buffalo (bubalus bubalis).in developing countries, livestock are often fed a high-lignin, low-nutrient diet that is rich in aromatic compounds. it is therefore important to understand the structure of the microbial community responsible for the metabolism of these substances. a metagenomic analysis was therefore carried out to assess the microbial communities associated with the liquid and solid fractions of rumen biomaterial from domestic mehsani buffalo (bubalus bubalis) fed with varying proportions of roughage. the ex ...201626711277
amnion epithelial cells of buffalo (bubalus bubalis) term placenta expressed embryonic stem cells markers and differentiated into cells of neurogenic lineage in vitro.aim of the present study was the isolation, culture, and characterization of amniotic membrane-derived epithelial cells (ae) from term placenta collected postpartum in buffalo. we found that cultured cells were of polygonal in shape, resistance to trypsin digestion and expressed cytokeratin-18 indicating that they were of epithelial origin. these cells have negative expression of mesenchymal stem cell markers (cd29, cd44, and cd105) and positive for pluripotency marker (oct4) genes indicated tha ...201626670951
differential developmental competence and gene expression patterns in buffalo (bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells.the developmental ability and gene expression pattern at 8- to 16-cell and blastocyst stages of buffalo (bubalus bubalis) nuclear transfer (nt) embryos from fetal fibroblasts (ffs), amnion mesenchymal stem cells (amscs) and in vitro fertilized (ivf) embryos were compared in the present studies. the in vitro expanded buffalo ffs showed a typical "s" shape growth curve with a doubling time of 41.4 h and stained positive for vimentin. the in vitro cultured undifferentiated amscs showed a doubling t ...201626660476
trehalose improves semen antioxidant enzymes activity, post-thaw quality, and fertility in nili ravi buffaloes (bubalus bubalis).our objectives were to study the effect of trehalose in extender on (1) antioxidant enzymes profile during cryopreservation (after dilution, before freezing, and after thawing), (2) in vitro quality (after thawing), and (3) in vivo fertility of nili ravi buffalo (bubalus bubalis) bull spermatozoa. semen samples (n = 20) from four buffalo bulls were diluted in tris-citric acid-based extender having different concentrations of trehalose (0.0, 15, 30, 45, and 60 mm) and frozen in french straws. at ...201626653954
dispositions of enrofloxacin and its major metabolite ciprofloxacin in thai swamp buffaloes.given the limited information available in this species, the aim of this study was to investigate the pharmacokinetic characteristics of enrofloxacin (er) and its major metabolite ciprofloxacin (cp) in buffaloes, bubalus bubalis. er was administered intravenously (i.v.) or subcutaneously (s.c.) to buffaloes at doses of 5.0 and 7.5 mg/kg bw, and plasma, urine and fecal samples were collected until 48 hr post-administration. the concentrations of er and cp in the plasma, urine and feces were analy ...201626596287
supplementation of slow-release melatonin improves recovery of ovarian cyclicity and conception in summer anoestrous buffaloes (bubalus bubalis).the role of melatonin as a protective neurohormone against restoring cyclicity in summer anoestrous animals in photoperiod species has gained wider acceptance. this study was designed to uncover the evidence the slow-release melatonin (mlt) has on initiation of ovarian cyclicity and conception rate (cr) in summer anoestrous buffaloes. thus, buffaloes diagnosed as summer anoestrous (absence of overt signs of oestrus, concurrent rectal examination and radioimmunoassay for serum progesterone at 10 ...201626566713
comparison of non-linear models to describe the lactation curves for milk yield and composition in buffaloes (bubalus bubalis).in order to describe the lactation curves of milk yield (my) and composition in buffaloes, seven non-linear mathematical equations (wood, dhanoa, sikka, nelder, brody, dijkstra and rook) were used. data were 116,117 test-day records for my, fat (fp) and protein (pp) percentages of milk from the first three lactations of buffaloes which were collected from 893 herds in the period from 1992 to 2012 by the animal breeding center of iran. each model was fitted to monthly production records of dairy ...201626354679
a comparative study on efficiency of adult fibroblasts and amniotic fluid-derived stem cells as donor cells for production of hand-made cloned buffalo (bubalus bubalis) embryos.the efficiency of two cell types, namely adult fibroblasts, and amniotic fluid stem (afs) cells as nuclear donor cells for somatic cell nuclear transfer by hand-made cloning in buffalo (bubalus bubalis) was compared. the in vitro expanded buffalo adult fibroblast cells showed a typical "s" shape growth curve with a doubling time of 40.8 h and stained positive for vimentin. the in vitro cultured undifferentiated afs cells showed a doubling time of 33.2 h and stained positive for alkaline phosphat ...201625501536
treatment of buffalo (bubalus bubalis) donor cells with trichostatin a and 5-aza-2'-deoxycytidine alters their growth characteristics, gene expression and epigenetic status and improves the in vitro developmental competence, quality and epigenetic status of cloned embryos.we examined the effects of treating buffalo skin fibroblast donor cells with trichostatin a (tsa), a histone deacetylase (hdac) inhibitor, and 5-aza-2'-deoxycytidine (5azadc), a dna methyltransferase (dnmt) inhibitor, on the cells and embryos produced by hand-made cloning. treatment of donor cells with tsa or 5azadc resulted in altered expression levels of the hdac1, dnmt1, dnmt3a, p53, caspase3 and caspase9 genes and global levels of acetylation of lysine at position 9 or 14 in histone 3 (h3k9/ ...201625409339
assessment of sperm damages during different stages of cryopreservation in water buffalo by fluorescent probes.the present study was designed to investigate the sperm damages occurring in acrosome, plasma membrane, mitochondrial activity, and dna of fresh, equilibrated and frozen-thawed buffalo semen by fluorescent probes. the stability of sperm acrosome and plasma membrane stability, mitochondrial activity and dna status were assessed by fluorescein conjugated lectin pisum sativum agglutinin, annexin-v/propidium iodide, jc-1 and tunel assay, respectively, under the fluorescent microscope. the damages pe ...201625373338
determination of naturally occurring estrogenic hormones in cow's and river buffalo's meat by hplc-fld method.this study was performed to measure and compare the levels of steroid hormones [estrone (e1), 17β-estradiol (e2), and estriol (e3)] and their conjugated metabolites in cow's and river buffalo's meat in two distinct follicular and luteal phases. moreover, the possible effect of a heating process on steroid hormone concentration was also investigated. the collected meat (biceps femoris muscle) samples were subjected to liquid extraction, enzymatical deconjugation, and c18 solid-phase extraction. e ...201628911549
seroepidemiology of infection withneospora caninum, leptospira, and bovine herpesvirus type 1 in water buffaloes(bubalus bubalis)in veracruz, mexico.we aimed to determine the seroprevalence of infection withneospora caninum, leptospira, and bovine herpesvirus type 1 and risk factors associated with these infections in water buffaloes in veracruz state, mexico. through a cross-sectional study, 144 water buffaloes(bubalus bubalis)raised in 5 ranches of veracruz were examined for anti-n.caninumand anti-bovine herpesvirus type 1 antibodies by enzyme immunoassays, andanti-leptospira interrogansantibodies by microscopic agglutination test. of the ...201729403656
posttranslational modifications in spermatozoa and effects on male fertility and sperm viability.spermatogenesis is a complex and highly regulated process. the ability of spermatozoa to perform its function depends on multiple physiological and genetic factors that are not fully understood. notably, due to lack of transcriptional and translational activity in spermatozoa, posttranslational modifications (ptms) play key roles in determining their viability. ptms not only confer structural changes in the proteome of the spermatozoa cells, but also increase the diversity of the proteome and in ...201728481731
effect of sexual excitation on testosterone and nitric oxide levels of water buffalo bulls (bubalus bubalis) with different categories of sexual behavior and their correlation with each other.we studied the effect of sexual excitation on serum testosterone and nitric oxide (no) levels in water buffalo bulls with different categories of sexual behavior and their correlation with each other. buffalo bulls were classified according to their sexual behavior (including reaction time, sexual aggressiveness and mating ability): acceptable (good to excellent) (n=5), fair (n=5), and unacceptable (poor) (n=5) sexual behavior. blood samples were collected from all animals immediately before and ...201728477950
ovulatory and fertility response using modified heatsynch and ovsynch protocols in the anovular murrah buffalo (bubalus bubalis).we studied the effect of modified heatsynch and ovsynch protocols on the ovulatory response (or), estrus induction rate (eir) and conception rate (cr) in the anovular postpartum murrah buffalo (n = 35). in the modified heatsynch protocol (group i; n = 12), buffaloes were given two gnrh at 2 h interval on treatment day 0, pgf (pgf2α) on day 7 and estradiol (e2) 1 mg on day 8. two ftai were done at 20 h intervals after e2 administration. in the modified ovsynch protocol (group ii; n = 15), gnrh wa ...201728460685
plasma proteome of buffaloes.the proteomic approach has aroused the interest of veterinary medicine researchers, especially regarding the production of biopharmaceuticals and diagnosis of diseases in farm animals. water buffaloes have gained prominence in the world economy due to the quality of their milk, meat, and leather, in addition to being an important donor of blood components. this work aimed to identify and characterize the proteins present in the blood plasma of murrah buffaloes (bubalus bubalis) through 2d electr ...201728452126
mastitis outcomes on pre-ovulatory follicle diameter, estradiol concentrations, subsequent luteal profiles and conception rate in buffaloes.the objectives of this study was to investigate the outcome of mastitis, in its clinical or subclinical forms, on the mean diameter of pre-ovulatory follicle (pof), plasma estradiol concentration on the day of estrus, subsequent luteal profile and subsequent conception rate in buffaloes. sixty dairy buffalo (bubalus bubalus) conducted in this study were divided into three groups {healthy (h), n=20; subclinical mastitis (scm), n=18; and clinical mastitis (cm), n=22}. ultrasonography of ovaries re ...201728442176
buffalo leukemia inhibitory factor induces differentiation and dome-like secondary structures in cos-1 cells.this study aimed to understand the molecular characteristics of buffalo leukemia inhibitory factor (bulif) and the generation of a stably transfected cos-1_bulif cell line for its functional characterization. cumulus cells, isolated from oocytes, were separated, and total cdna was prepared. the bulif gene was ligated into the cloning vector pjet1.2/blunt and expression vector pacgfp-n1 which was transfected into cos-1 cells and confirmed by qrt-pcr and western blot. bulif was immunoprecipitated ...201728441662
characterization of leukocyte subsets in buffalo (bubalus bubalis) with cross-reactive monoclonal antibodies specific for bovine mhc class i and class ii molecules and leukocyte differentiation molecules.although buffaloes (bubalus bubalis) are a major component of the livestock industry worldwide, limited progress has been made in the study of the mechanisms regulating the immune response to pathogens and parasites affecting their health and productivity. this has been, in part, attributable to the limited availability of reagents to study immune responses in buffalo. as reported here, a set of cross-reactive monoclonal antibodies (mabs), developed against bovine, ovine and caprine leukocyte di ...201728433527
intermolecular interactions between dmα and dmβ proteins in bula-dm complex of water buffalo bubalus bubalis.the major histocompatibility complex (mhc) with its three classes represents a cluster of tightly linked genes with defined immunological and non-immunological functions. the dm, a mhc class ii molecule is formed by the non-covalent association of dmα and dmβ chains. it binds with the processed peptide antigens and presents them to t lymphocytes, thereby triggering the immune responses. startlingly, the expression pattern and structural organization of dmα and dmβ proteins in buffalo remains und ...201728419545
retinoic acid induces differentiation of buffalo (bubalus bubalis) embryonic stem cells into germ cells.development of precise and reproducible culture system for in vitro differentiation of embryonic stem (es) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by limited availability of precursor primordial germ cells (pgcs), but in finally treating the catastrophic infertility. taking into account the significant role of retinoic acid (ra) during in vivo gametogenesis, we designed the present study to invest ...201728736154
genomic differentiation between swamp and river buffalo using a cattle high-density single nucleotide polymorphisms panel.buffalo (bubalus bubalis) is an important livestock species in many tropical and subtropical regions. in recent decades, the interest in buffalo's milk have expanded and intensive buffalo farms start to emerge. however, breeding programs and population genetics information for this species is scarce or inexistent. the present study aims to test the suitability of the commercial high-density single nucleotide polymorphisms (snp) genotyping panel, the illumina bovinehd beadchip, to estimate popula ...201728735584
influence of body condition score at calving on the metabolic status and production performance of murrah buffaloes (bubalus bubalis) during the transition period.the purpose of this study was to evaluate the influence of body condition score (bcs) at calving on the metabolic status of female murrah buffaloes in the transition period.201728728402
ultrastructure changes in buffalo (bubalus bubalis) oocytes before and after maturation in vitro with sericin.the aim of this research was to identify the changes in the cytoplasmic ultrastructure of immature and matured oocytes in buffalo (bubalus bubalis). oocytes were matured in vitro in tissue culture medium-199 with and without sericin, and then analyzed by light and transmission electron microscopy. the experiment result showed that the nuclear maturation rate of buffalo oocytes was significantly higher in the presence of sericin (80.6%) than without sericin (68.1%) (p < 0.05). the immature oocyte ...201728722264
expression and molecular cloning of interferon stimulated genes in buffalo (bubalus bubalis).buffalo, the most important livestock species in tropical india, remains to be a poor breeder mainly due to embryonic mortality (65%) occurring mostly between 16 and 18 days of pregnancy. early and accurate diagnosis of pregnancy can thus become a boon for successful herd management in buffalo. however, most of the currently available methods allow diagnosis only after 30 days post ai. interferon tau (ifnt), the first pregnancy recognition signal in ruminants is one such molecule, which stimulat ...201728708533
whole mitogenomes reveal the history of swamp buffalo: initially shaped by glacial periods and eventually modelled by domestication.the newly sequenced mitochondrial genomes of 107 asian swamp buffalo (bubalus bubalis carabensis) allowed the reconstruction of the matrilineal divergence since ~900 kya. phylogenetic trees and bayesian skyline plots suggest a role of the glacial periods in the demographic history of swamp buffalo. the ancestral swamp-buffalo mitogenome is dated ~232 ± 35 kya. two major macro-lineages diverged during the 2nd pleistocene glacial period (~200-130 kya), but most (~99%) of the current matrilines der ...201728680070
comparative efficacy of oestrus synchronization protocols in buffalo (bubalus bubalis).this study was conducted to test the efficacy of gonadotropic hormone (gnrh)-based synchronization protocols (ovsynch, heatsynch, and ovsynch plus) in buffaloes under field condition. based on anamnesis and transrectal palpation twice at 10-day interval and serum progesterone (p4) concentration, 150 anoestrous buffaloes and delayed pubertal heifers were selected to induce oestrus using gnrh-based protocols. these selected animals were randomly divided into three groups: group i: ovsynch (n = 50) ...201728669062
ovum pick-up interval in buffalo (bubalus bubalis) managed under wetland conditions in argentina: effect on follicular population, oocyte recovery, and in vitro embryo development.the excellent adaptation of water buffalo (bubalis bubalis) to swampy environments means that animals are frequently managed in areas with restricted access for reproductive procedures. the objective of this study was to evaluate the effect of the ovum pick-up (opu) interval on follicular population, oocyte recovery, oocyte quality and in vitro embryo production. twelve murrah buffaloes were subjected to two consecutive dominant follicle reductions, and randomly assigned to either 7-day (n=6) or ...201728662882
cryopreservation of nili-ravi buffalo (bubalus bubalis) semen in andromed® extender; in vitro and in vivo evaluation.the study was designed to evaluate andromed® for the freezability and fertility of nili-ravi buffalo semen. semen was collected from four adult nili-ravi buffalo (bubalus bubalis) bulls for 3 weeks (replicate). semen ejaculates from each buffalo bull were divided into three aliquots. one aliquot was used for evaluation of motility, plasma membrane integrity, livability, viability, dna integrity and normal apical ridge. remaining two aliquots were diluted (37°c; 50 × 106 spermatozoa/ml) in tris-c ...201728660630
expression, localization of sumo-1, and analyses of potential sumoylated proteins in bubalus bubalis spermatozoa.mature spermatozoa have highly condensed dna that is essentially silent both transcriptionally and translationally. therefore, post translational modifications are very important for regulating sperm motility, morphology, and for male fertility in general. protein sumoylation was recently demonstrated in human and rodent spermatozoa, with potential consequences for sperm motility and dna integrity. we examined the expression and localization of small ubiquitin-related modifier-1 (sumo-1) in the ...201728659810
pcr-sscp analysis of mdgi gene and its association with milk production traits in river buffalo (bubalus bubalis).in this study, we investigated the genetic variation within 3'utr of mammary-derived growth inhibitor (mdgi) gene of buffalo using pcr-sscp and sequencing; and also analyzed association of polymorphism with the milk production traits. the study revealed two conformational patterns, 'a' and 'b' among 234 mehsana buffaloes maintained with their records in the field and at farm. the frequency of sscp variant 'a' was found to be invariably high in the buffalo population under study. further, associa ...201728647599
prevalence and microscopic studies of sarcocystis infection in naturally infected water buffaloes (bubalus bubalis) of andhra pradesh.sarcocystis species are the obligate tissue protozoan parasites of livestock causing clinical and subclinical disease resulting in downgrading of the meat and their products thereby leading to economic losses. the present study reveals the prevalence and distribution of sarcocystosis in water buffaloes (bubalus bubalis) slaughtered at local abattoirs in a.p for a period of 1 year from june 2014 to may 2015. a total of 137 buffalo carcasses were screened grossly and microscopically organ wise viz ...201728615863
pregnancy-associated glycoproteins (pags) concentrations in water buffaloes (bubalus bubalis) during gestation and the postpartum period.for the first time in literature this study describes the pregnancy-associated glycoprotein (pag) profile of buffalo cows during gestation and the post-partum period using antiserum raised against pag-molecules purified from buffalo placenta (as#860). ninety-eight buffalo cows, belonging to a buffalo herd subjected to a synchronization and artificial insemination (ai) program, were enrolled in this study. blood samples were taken on days 0 (ai), 23, 25, 28, 30 and then biweekly until the end of ...201728583611
hematologic ris for healthy water buffaloes (bubalus bubalis) in southern mexico.baseline hematologic data are used by veterinarians and wildlife biologists to perform health assessments on target animal species. hematologic measurements are influenced by various factors including geography. baseline hematologic ris have been established for domestic water buffaloes (bubalus bubalis) from various countries in the eastern hemisphere, but these data are not readily available for the western hemisphere.201728582601
comprehensive characterization of bioactive peptides from buffalo (bubalus bubalis) colostrum and milk fat globule membrane proteins.milk fat is dispersed in milk as small spherical globules stabilized in the form of emulsion by its surrounding membrane, often referred to as fat globule membrane (fgm). buffalo, a major milking mammal of asia and second most milking mammal across the globe presents physicochemical features different from that of other ruminant species containing higher content of lipids and proteins. the present study describes characterization of fgm proteins isolated from both buffalo milk and colostrum. a d ...201728578070
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