Publications

a marmoset t lymphoma which functions as a human amplifier t cell.	a long-term in vitro grown t cell line derived from a cotton-topped marmoset (saguinus oedipus) infected with herpesvirus saimiri was fond to share surface antigens with human amplifier t cells and to augment the capacity of human b cells to secrete immunoglobulin. this is the first demonstration of t/b collaboration across such a large phylogenetic barrier and might have interesting implications for understanding the nature of molecular interactions mediating cell/cell cooperation.	1981	6801144
changes in immune response during responses in ms patients.	changes in clinical status and in two measures of immune function were followed for 21 months (median) in 106 multiple sclerosis (ms) patients and cohabitant controls. antibody titers to measles, cytomegalovirus, and herpesvirus 1 and 2, and leukocyte migration inhibition indexes (lmis) to measles and streptokinase/streptodornase (sksd) were measured at 3- to 6-month intervals and at time of exacerbation in the index case. there were 36 exacerbations in 25 patients. mean baseline antibody titers ...	1981	6452588
[malignant fibrous histiocytoma of the bone].	the data of clinico-morphological study of 10 observations of malignant fibrous histiocytoma of the bone indicating its distinct nosological importance are presented. clinico-morphological comparisons showed the typical variant of the morphological structure to have the worst prognosis. in two observations, the tumor was examined electron microscopically. all the 5 main types of cells were found: histiocyte- and fibroblast-like, giant multinuclear, xanthomatous, and poorly-differentiated mesench ...	1981	6274277
herpesvirus saimiri dna in tumor cells--deleted sequences and sequence rearrangements.	herpesvirus saimiri dna in continuous lymphoblastoid cell lines obtained from viral induced tumors in marmosets has been analyzed by gel electrophoresis of restricted dna. southern transfer to nitrocellulose filters, and hybridization to 32p-labeled viral dna or dna fragments. the viral dna fragments ecori-g, -h, -d, and -i, kpni-a, and bamhi-d and -e were not detected in southern transfers of dna from the nonproducing 1670 cell line. for each restriction endonuclease, a new fragment appeared, c ...	1981	6268839
infection of multiple t-cell subsets and changes in lymphocyte functions associated with herpesvirus saimiri infection of owl monkeys.	we examined the association of herpesvirus saimiri with lymphocyte subsets and the functional integrity and distribution of these populations in owl monkeys with chronic, disease-free infections, in uninoculated, control animals, and in one monkey with h. saimiri-induced lymphoma. the lymphocyte subpopulations examined included total t cells, t cells with receptors for the fc portion of immunoglobulin g (the tgamma cells), t cells lacking this receptor (the tgamma(-) population), and non-t cells ...	1981	6265360
herpesvirus (hv) infection in newborns.		1981	6264286
an interchangeable elisa for cytomegalovirus antigen and antibody.	an enzyme-linked immunosorbent assay (elisa) was developed to demonstrate cytomegalovirus (cmv) antibodies and antigen. a nuclear antigen from cmv-infected cells was used for detection of igg and igm antibodies. significant rises of cmv-igg and significant levels of cmv-igm were found in sera from patients with acute cmv only, and not with other herpesvirus infections. cmv antigens were demonstrated in cells and in culture medium by a sandwich or by an inhibition elisa technique. in the sandwich ...	1981	6262352
herpesvirus ateles and herpesvirus saimiri transform marmoset t cells into continuously proliferating cell lines that can mediate natural killer cell-like cytotoxicity.	herpesvirus ateles (hva) and herpesvirus saimiri (hvs) have the capacity to transform cotton-topped marmoset t lymphocytes into continuously proliferating cell lines that retain some functions associated with cell-mediated immunity. in the present paper, we demonstrate that hva/hvs-transformed t cell lines are cytotoxic in a short-term 51cr release assay and that this killing resembles killing by marmoset natural killer (nk) cells. the relationship between nk cells and hva/hvs-transformed killer ...	1981	6273869
incidence of rotavirus, adenovirus and herpesvirus infection in pigeons.		1981	6278694
caprine herpesvirus infection in switzerland: some aspects of its pathogenicity.		1981	6278798
spontaneous generalized herpesvirus hominis infection of a lowland gorilla (gorilla gorilla gorilla).	a 13-day-old lowland gorilla died from a generalized herpesvirus infection shortly after the onset of clinical signs. the pathologic-anatomical findings were compatible with those described for generalized herpes simplex infection in the human neonate. electron microscopic examination of lung tissue revealed the presence of herpesvirus which was identified with the fluorescent antibody technique as herpes simplex virus type i. tests with related sera of the herpes group (varicella, herpesvirus-b ...	1981	6279853
an outbreak of paresis in mares and geldings associated with equid herpesvirus 1.	an outbreak of paresis occurred on a small isolated stud farm in july 1980. of the 42 horses on the stud, infection was confined to a group of nine in-foal mares and their foals and eight other horses which were either housed together at night or grazed adjacent pastures. eight mares and two geldings developed ataxia or paresis and one mare died. equid herpesvirus 1 was isolated from 17 animals and serological studies confirmed that 24 of 26 animals sampled had experienced infection.	1981	6280366
neonatal infection with mouse thymic virus: spleen and lymph node necrosis.	mouse thymic virus (ta) is a naturally occurring herpesvirus of laboratory and wild mice, which produces massive thymic necrosis when inoculated into newborn mice. our histopathological study showed necrosis not only of the thymus but also of the spleen and lymph nodes which was noticeable by day 7 and complete by day 14. both spleen and lymph nodes regenerated to an almost normal histological pattern by day 70. the results show that ta infects multiple lymphoid tissues causing massive necrosis ...	1981	6275013
analysis of the genome of equine herpesvirus type 1: arrangement of cleavage sites for restriction endonucleases ecori, bglii and bamhi.	the genome of an australian isolate of equine herpesvirus type 1 (equine abortion virus) has been analysed using the restriction endonucleases ecori, bglii and bamhi, and a physical map constructed. terminal fragments were identified by exonuclease treatments, and linkage of fragments was deduced by a combination of single- and double-digest experiments and cross-blot hybridizations. the genome has a mol. wt. of 100 x 10(6) and is comprised of a short unique region bounded by repetitive sequence ...	1981	6275028
proteins specified by bovine herpesvirus 1 (infectious bovine rhinotracheitis virus).	an electrophoretic analysis of radioactively labeled, purified, "empty" and dna-containing infectious bovine rhinotracheitis virions revealed the presence of 25 to 33 structural (virion) polypeptides. a total of 11 of these polypeptides could be labeled with [3h]glucosamine and were identified as glycoproteins. in addition to the 25 structural polypeptides, infectious bovine rhinotracheitis virus infected cells also contained at least 15 nonstructural (nonvirion) polypeptides that were not prese ...	1981	6275094
fatal herpesvirus tamarinus infection in cotton-topped marmosets (saguinus oedipus).		1981	6275150
[herpesvirus infections in puppies: virological data and histopathological findings].		1981	6275500
rapid detection of viruses in biopsy or autopsy specimens by the pseudoreplica method of electron microscopy.	a rapid pseudoreplica method of electron microscopy (em) was used to detect viruses in brain tissues obtained from 57 patients suspected of having herpes simplex encephalitis (hse). herpesvirus particles were demonstrated in 18 of the 20 (90%) specimens from which herpes simplex virus was isolated in tissue culture. the procedure takes less than 2 hours, as compared to the time (greater than or equal to 2 days) usually required for virus isolation or standard thin-section electron microscopy. al ...	1981	6275724
viral antibody studies of laboratory dogs with diarrheal disease.	viral antibody studies were done on laboratory dogs in an epizootic of gastrointestinal disease. increased hemagglutination-inhibition antibody titers to a parvovirus (pv) antigenically related to feline panleukopenia virus were found in convalescent serum specimens of 78% (20/26) of the affected dogs and in 83% (5/6) of apparently healthy dogs. with one exception, all dogs tested had significant levels of hemagglutination-inhibition antibody to this pv. similar increased antibody titers were fo ...	1981	6275746
further studies of naturally occurring latent bovine herpesvirus infection.	attempts were made to enhance the isolation rate of latent bovine herpesviruses from trigeminal ganglia by using a fibroblastic fetal bovine kidney cell line, in addition to madin-darby bovine kidney cells, and by superinfecting with a temperature-sensitive helper strain of bovine herpesvirus-1 (bhv-1). four isolates of latent bhv-1 were obtained from 44 pairs of trigeminal ganglia--thus reflecting no increase in isolation rate over that previously observed. two isolates of a latent bovine herpe ...	1981	6275750
studies on benzhydrazone, a specific inhibitor of herpesvirus glycoprotein synthesis. size distribution of glycopeptides and endo-beta-n-acetylglucosaminidase-h treatment.	benzhydrazone is a bis-amidinohydrazone derivative which specifically hinders the formation of herpes simplex virus (hsv) glycoproteins. in this study we present some structural features of the oligosaccharide chains of herpesvirus glycoproteins synthesized in cells incubated with the inhibitor. gel filtration analysis of glycopeptides, obtained through exhaustive pronase-digestion of infected cells after a long or a short labeling with 14c-glucosamine, showed that benzhydrazone reduced the appe ...	1981	6275822
postexposure immunoprophylaxis against b virus (herpesvirus simiae) infection.	local infiltration of antiserum into sites inoculated with b virus protected rabbits from an otherwise fatal encephalomyelitis. treatment was effective when delayed for six hours but not after 24 hours. homologous rabbit antisera were more effective than heterologous monkey antisera, and protection was unrelated to neutralisation titres. protection apparently depended not on neutralisation of inoculated virus but on destruction of infected cells before they produced progeny virus. normal human i ...	1981	6275934
the site of integration of the herpes simplex virus type 1 thymidine kinase gene in human cells transformed by an hsv-1 dna fragment.	to analyze the site of integration of the herpes simplex virus type i (hsv-i) thymidine kinase (tk) gene in biochemically transformed human cells, tk-hela-(bu25) cells were transformed to the tk+ phenotype by a cloned, 2 kbp pvull fragment of hsv-i dna. the transformed cells [hela(bu25)/tf pago pp3] were fused with mouse lm(tk-) cells, and human-mouse somatic cell hybrid clones (lh pp3 clones 1, 2, 3, 5 and 6) were isolated in hatg-ouabain selective medium. the hela(bu25)/tf pago pp3 cells and t ...	1981	6277799
dna-polymerase induced by herpesvirus papio (hvp) in cells of lymphoblastoid cultures derived from lymphomatous baboons. report v.	a new dna-polymerase was found in the cells of suspension lymphoblastoid cultures which produce lymphotropic baboon herpesvirus (hvp). this enzyme was isolated in a partially purified form. some of its properties vary from those of other cellular dna-polymerases. hvp-induced dna-polymerase has a molecule weight of 160,000 and sedimentation coefficient of about 8 s. the enzyme is resistant to high salt concentration and n-ethylmaleimide, but it is very sensitive to phosphonoacetate. it effectivel ...	1981	6276214
fatal herpesvirus infection in patas monkeys and a black and white colobus monkey.	fatal herpesvirus infections were diagnosed in 3 patas monkeys and 1 black and white colobus monkey over a 4-week period. herpesvirus was isolated from 1 patas monkey and from the black and white colobus monkey. both isolates had growth characteristics similar to herpesvirus hominis and herpesvirus simiae. the isolate from the colobus monkey antigenically appeared to be h simiae or h simiae-like, whereas the isolate from the patas monkey could not be conclusively identified with the antisera use ...	1981	6276349
[preliminary seroepizootiological studies of the distribution of caprine herpesvirus infections in switzerland].		1981	6278582
[characterization of bovine herpesvirus type i puente alto chile 1977 strain].		1981	6285438
difference between influences of homologous and heterologous maternal antibodies on response to serotype-2 and serotype-3 marek's disease vaccines.	marek's disease (md) vaccines representing serotypes 2 (sb-1 strain of md virus) and 3 (fc-126 strain of turkey herpesvirus) were administered to 1-day-old md-susceptible chicks that either were free of antibodies or carried maternally derived antibodies against sb-1, fc-126, or a serotype-1 md virus, cu-2 strain. homologous antibodies delayed the development of vaccine virus viremias and inhibited vaccinal immunity, as judged by protection against challenge with the virulent jm-10 strain of md ...	1981	6268052
herpesvirus hominus (herpes simplex virus).		1981	6268353
pathogenesis of pigeon herpesvirus infection.		1981	6274925
herpesvirus as a cause of fatal disease in australian wallabies.		1981	6274926
canine viruses and multiple sclerosis.	serum and cerebrospinal fluid from multiple sclerosis (ms) patients and control subjects were tested and compared for presence and titer of neutralizing antibody against the most common canine viruses. canine viruses included canine distemper virus (cdv), canine adenovirus 1 (cav-1), canine parainfluenza virus (cpiv), canine herpesvirus (chv), canine coronavirus (ccv), and canine parvovirus (cpv). neutralizing titers against measles virus (mv) and human adenovirus 8 (ha8) were also tested. signi ...	1981	6267515
serological responses to mycoplasma synoviae in chickens infected with virulent or avirulent strains of marek's disease virus.	the pathogenicity and immunosuppressive capability of five strains of marek's disease herpesvirus (mdhv) was examined. three of the strains (ala, miss, ncs) were isolated from flocks experiencing vaccine breaks while the other two strains (ga, hn) represented prototype virulent and avirulent viruses. pathogenicity of mdhv strains was evaluated on their ability to cause mortality, their ability to cause gross or microscopic lesions, and the median latent period to death (mlpd) for each strain. an ...	1981	6267580
cloning of the marmoset herpesvirus thymidine kinase gene and analyses of the boundaries of the coding region.		1981	6267782
inhibitory effects of foscarnet on herpesvirus multiplication in cell culture.	the effect of phosphonoformate (inn; foscarnet sodium) on herpes simplex virus type 1 replication in cell-culture has been studied. at 55 microm, foscarnet reduced the yield by 50 per cent which correlated well with a 50 per cent reduction of plaque formation at 60 microm. foscarnet had to be added before 8 hours post infection to inhibit virus production. the inhibition of herpesvirus plaque formation by the presence of foscarnet for 24 hours was not reversed by the removal of the drug. the inh ...	1981	6268019
the detection and behaviour of the herpesvirus of malignant catarrhal fever in bovine lymphocytes.		1981	6268024
herpesvirus-induced antigens in squamous-cell carcinoma in situ of the vulva.	antigens induced by herpes simplex virus type 2 (hsv2) were found to be associated with squamous-cell carcinoma in situ of the vulva in nine of 10 patients. the hsv2-induced antigens are dna-binding proteins that are normally present in the nuclei of infected cells, but in the cells of the carcinomas in situ they were found in the cytoplasm. whole-virion structural antigens were not present, although there was serologic evidence of previous hsv2 infection in patients tested for the presence of a ...	1981	6265780
expression of herpesvirus-induced antigens in human cervical cancer.		1981	6265797
on the mechanism of selective inhibition of herpesvirus replication by (e)-5-(2-bromovinyl)-2'-deoxyuridine.	bromovinyldeoxyuridine (bvdurd) is a potent antiherpesvirus compound with low cytotoxicity. to gain an insight into its selectivity and mechanism of inhibition, we chemically synthesized the 5'-triphosphate of bvdurd, bvdutp, and tested its effect on the activities of dna polymerases [dna nucleotidyltransferase (dna directed), ec 2.7.7.7] of two herpesviruses--i.e., herpes simplex virus type 1 (hsv-1) and epstein-barr virus (ebv)--as well as cellular dna polymerases alpha, beta, and gamma. the e ...	1981	6265902
differentiation of sub-types of equine herpesvirus i by restriction endonuclease analysis.		1981	6266385
mortalities associated with herpesvirus infection in captive macropods.	fatal cases of herpesvirus infection in a quokka (setonix brachyurus), eight grey dorcopsis wallabies (dorcopsis muelleri luctuosa) in two separate outbreaks, as well as presumptive fatal herpesvirus infection in a western grey kangaroo (macropus fuliginosus) are described. all were captive animals and deaths were preceded by a period of stress. lesions occurred most often in alimentary tract epithelium, respiratory tract, skin, genital epithelium, conjunctivae, liver and adrenal cortex. herpesv ...	1981	6264169
detection of antibodies to herpesvirus simiae and herpesvirus hominis in nonhuman primates.	sera from nonhuman primates, predominantly macaca species, were assayed by a serum neutralization test for antibodies to antigenically related herpesvirus simiae (b virus) and herpesvirus hominis type 1. the data indicate that there would have been approximately 50% error in the diagnosis of herpesvirus simiae infection if these sera had been tested only against herpesvirus hominis antigen. the role of active guinea pig complement in the serum neutralization test was also evaluated and found to ...	1981	6264222
risk of recurrence after first episodes of genital herpes. relation to hsv type and antibody response.	to define risk factors associated with recurrent genital herpes-simplex-virus infection caused by either type 1 or 2 herpesvirus (hsv-1 or hsv-2), we prospectively studied 137 patients with a first symptomatic episode of the disease and 87 with a recurrent episode. first episodes were divided into 78 primary infections (no antibodies to hsv in acute-phase serum) and 59 nonprimary infections (antibodies present). hsv-1 infections were less frequent and less likely to recur than hsv-2 infections. ...	1981	6264300
herpes simplex encephalitis. a modified indirect immunoperoxidase technique for rapid diagnosis in paraffin-embedded tissue.	a modification of a reliable and simple method of the indirect immunoperoxidase technique was used to identify herpesvirus antigen in paraffin-embedded tissue of patients with herpes simplex encephalitis (hse), as well as in rats and mice. so far we have studied five cases in humans and six in mice and rats. with preincubation, we increased antigenicity and accomplished high specificity. brown reaction product was found mainly at the periphery of the areas of necrotizing encephalitis. here it wa ...	1981	6264883
detailed characterization of the mrna mapping in the hindiii fragment k region of the herpes simplex virus type 1 genome.	we have isolated as recombinant dna clones, in the plasmid pbr322, regions of the herpesvirus type 1 genome spanning the region between 0.53 and 0.6 on the prototypical arrangement. this 11,000-base-pair region corresponds to 10% of the large unique region and encodes five major and several minor mrna species abundant at different times after infection, which range in length from 7 to 1 kilobase. in this report, we have used rna transfer blots and s1 nuclease digestion of hybrids between viral d ...	1981	6262521
herpesvirus-transformed cytotoxic t-cell lines.	investigations of cellular cytotoxicity of the immune system are hampered by the lack of continuously growing, transformed cell lines which express a cytotoxic potential. here we describe cytotoxic cell lines from the cotton-topped marmoset monkey, transformed by herpesvirus ateles (hva) or herpesvirus saimiri (hvs), which can kill certain target cells in a short-term in vitro test. hva/hvs-transformed cells have earlier been classified as belonging to the t-cell lineage in contrast to epstein-b ...	1981	6262648
serological relationship between abortifacient and respiratory strains of equine herpesvirus type 1 in new zealand.		1981	6262693
two small rnas encoded by epstein-barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus.	primate cells harboring the epstein-barr virus (ebv) genome synthesize large amounts of two small rnas:eber 1 and eber 2 (ebv-encoded rna). these rnas are approximately 180 nucleotides long, possess 5' pppa termini, and lack poly(a). they have different t1 and pancreatic rnase digestion fingerprints. they are not found in normal b lymphocytes, in transformed b lymphocytes that lack ebv dna, in t lymphocytes transformed by herpesvirus ateles, or in a variety of other nonlymphoid mammalian cells. ...	1981	6262773
nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.	we have determined the complete nucleotide sequence of the thymidine kinase (atp:thymidine 5' phosphotransferase, ec 2.7.1.21) gene of herpes simplex virus type 1 strain cl101 from a plasmid clone of viral dna derived by enquist et al. [enquist, l. w., vande woude, g. f., wagner, m., smiley, j. r. & summers, w. c. (1979) gene 7, 335-342]. a cdna copy of the 5' end of thymidine kinase mrna was also analyzed to locate the transcribed sequences. the transcribed portion of the gene is approximately ...	1981	6262799
experimental study of contagious paralysis of pigeons.	a study of the clinical and pathological changes in pigeons experimentally inoculated with a herpesvirus isolated recently from natural cases of a condition termed contagious paralysis was carried out. signs and pathological findings similar to those recorded in natural cases were produced after inoculation of the isolate via different routes. the incubation period differed according to the route of inoculation from 2 to 10 days and the course of the disease from 5 to 21 days. the mortality rang ...	1981	6262960
multifocal varicella-zoster virus leukoencephalitis temporally remote from herpes zoster.	two patients with cancer, one with hodgkin's disease and the other with a granulosa cell tumor of the ovary, developed a progressive, eventually fatal infection of the central nervous system exhibiting multifocal symptoms and signs. pathologically, gross abnormalities of the brain resembled those in progressive multifocal leukoencephalopathy (pml), with discrete and confluent plaque-like lesions concentrated in the white matter, particularly along the gray-white junction. microscopically, pathol ...	1981	6261672
enhancement of varicella-zoster virus replication in cultured human embryonic lung cells treated with the pesticide carbaryl.	in studies designed to determine the factors responsible for control of herpesvirus replicaton in an infected cell, we examined the interaction of varicella-zoster (vz) virus-infected human embryonic lung cells with the pesticide carbaryl. the replication of the cell-associated vz virus was enhanced 2- to 13-fold as compared to control cultures in sevin 4 oil-treated cultures and in cultures treated with the pesticide's active ingredient, carbaryl. the replication of vz virus in cultures teated ...	1981	6261690
serological responses to the cell associated herpesvirus and the manx calicivirus of spf male cats with herpesvirus-induced urolithiasis.	the feline cell associated herpesvirus (cahv), but not the manx calicivirus, was previously reported to induce urolithiasis in specific pathogen free (spf) cats. serum neutralization (sn) antibody studies, reported here, revealed that the experimental spf cats did not have sn antibodies either against the cahv or the manx calicivirus in preinoculation serum samples. however, all cats inoculated with the cahv (either alone or in combination with the manx virus) developed sn antibodies against the ...	1981	6262014
complement requirement for virus neutralization by antibody and reduced serum complement levels associated with experimental equine herpesvirus 1 infection.	pony foals, negative for detectable serum-neutralizing antibody to equine herpesvirus 1 by the standard tube-culture virus neutralization test, were experimentally infected with equine herpesvirus 1. complement-requiring (cr) and non-complement-requiring (ncr) serum-neutralizing antibodies were evaluated in preinfection and postinfection sera by means of a complement-enhanced plaque reduction assay. low levels of cr antibodies were found in the preinfection sera of only group ii ponies. upon inf ...	1981	6260672
herpesvirus papio dna is similar in organization to epstein-barr virus dna.	ecori, hindii, sali, nd xbai restriction endonuclease maps of herpesvirus papio (hvpapio) dna were derived by determining the fragment sizes and the linkage relationships between fragments generated by the different enzymes. the data indicate that hvpapio dna has a single molecular arrangement which is similar to that of epstein-barr virus dna. the size of the dna was 110 x 10(6) to 114 x 10(6) daltons. restriction fragments from both ends varied in the number of repeats of a 4 x 10(5)-dalton se ...	1981	6261014
linkage map of the fragments of herpesvirus papio dna.	herpesvirus papio (hvp), an epstein-barr-like virus, causes lymphoblastoid disease in baboons. the physical map of hvp dna was constructed for the fragments produced by cleavage of hvp dna with restriction endonucleases ecori, hindiii, sali, and pvui, which produced 12, 12, 10, and 4 fragments, respectively. the total molecular size of hvp dna was calculated as close to 110 megadaltons. the following methods were used for construction of the map; (i) fragments near the ends of hvp dna were ident ...	1981	6261015
colinearity between the dnas of epstein-barr virus and herpesvirus papio.	epstein barr virus (ebv) and herpesvirus papio (hvpapio) dnas share a common format and 40% homology. labeled cloned fragments of ebv dna were hybridized to blots of xbai, ecori, hindiii, and sali fragments of hvpapio dna. ebv fragments which mapped from 2 x 10(6) to 54 x 10(6) and from 59 x 10(6) to 106 x 10(6) daltons hybridized to fragments at identical map positions in hvpapio dna. regions of nonhomology were demonstrated at 0 x 10(6) to 2 x 10(6), 54 x 10(6) to 59 10(6), and 106 x 10(6) to ...	1981	6261021
latency of herpesvirus of turkey and marek's disease virus genomes in a chicken t-lymphoblastoid cell line.	the properties of latent herpesvirus of turkey (hvt) and marek's disease virus (mdv) genomes have been studied in virus-non-producer mdcc-bo1(t) cells, a t-lymphoblastoid cell line derived from spleen cells of an hvt-vaccinated chicken. the numbers of the two virus genomes in bo1(t) cells remained stable at 1.6 to 1.8 hvt genome equivalents/cell and 3.4 to 3.8 mdv genome equivalents/cell throughout a number of passages and were not decreased by the presence of phosphonoacetic acid in the culture ...	1981	6268738
[experimentally induced herpesvirus sa 8-pneumonia (bronchopneumonia) in kenya baboons (papio cynocephalus) (author's transl)].	captive baboons of three age groups were experimentally infected with herpesvirus sa 8-strain 0430. intravenous inoculation of the virus induced minor, transient, interstitial pneumonia of a nonspecific type in newborn baboons. intratracheal inoculation, in contrast, invariably produced multifocal or diffuse necrotizing inclusion body bronchopneumonia within two days in newborn, two months and one year old baboons. differences in the outcome of the experimental intratracheal infections were noti ...	1981	6269276
efficacy of acyclovir against mouse cytomegalovirus in vivo.	acyclovir reduced mortality and organ virus titers in mice inoculated intraperitoneally with 10 50% lethal doses of mouse cytomegalovirus. this susceptibility to acyclovir of a herpesvirus which lacks thymidine kinase is surprising. alternative phosphorylating enzymes may account for this susceptibility.	1981	6269490
a study in calves of an immunologic relationship between herpes simplex virus and bovid herpesvirus 2.		1981	6269796
genetic resistance to herpesvirus-induced lymphoma at the level of the target cell determined by the thymic microenvironment.		1981	6270018
immunofluorescent staining of nuclear antigen in lymphoid cells transformed by herpesvirus papio (hvp).	an improved fixation method for antigen detection in lymphoblastoid cells is described. herpesvirus papio nuclear antigen (hupna) could be stained in several transformed lymphoid cell lines by anti-complement immunofluorescence (acif). antibody to hupna was detected in many human sera containing antibodies to epstein-barr virus capsid and nuclear antigen (ebna). rheumatoid arthritis sera showed a high incidence of both anti-ebna and anti-hupna antibodies.	1981	6270210
morphological components of herpesvirus. iii. localization of herpes simplex virus type 1 nucleocapsid polypeptides by immune electron microscopy.	herpes simplex virus type 1 (hsv-1) nucleocapsids were observed in the electron microscope after their reaction with igg's purified from the sera of rabbits immunized with the individual nucleocapsid polypeptides. the combining sites of nc1, the major capsid protein (mol. wt. 154k), were distributed over the entire capsid surface. this result provides further evidence that nc1 represents the major hexamer constituent. nc2 (mol. wt. 50k) was less widely distributed and appeared to be located at c ...	1981	6270247
thymidine kinase not required for antiviral activity of acyclovir against mouse cytomegalovirus.	previous studies of herpesvirus infections have indicated that a virus-specified thymidine kinase is required for the initial phosphorylation of acyclovir [acycloguanosine or 9-(2-hydroxyethoxymethyl)guanine] in the formation of acycloguanosine triphosphate. the latter compound accumulates in infected cells and competitively inhibits the viral dna polymerase. we found that mouse cytomegalovirus, which does not express a thymidine kinase, was sensitive to the antiviral effects of acyclovir at a 5 ...	1981	6270357
herpes simplex virus types 1 and 2 completely help adenovirus-associated virus replication.	in addition to adenoviruses, which are capable of completely helping adenovirus-associated virus (aav) multiplication, only herpesviruses are known to provide any aav helper activity, but this activity has been thought to be partial (i.e., aav dna, rna, and protein syntheses are induced, but infectious particles are not assembled). in this study, however, we show that herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) are in fact complete aav helpers and that aav type 2 (aav2) infectivity yi ...	1981	6270377
regulation of herpesvirus macromolecular synthesis: temporal order of transcription of alpha genes is not dependent on the stringency of inhibition of protein synthesis.	operationally, alpha genes of herpes simplex virus 1 were defined on the basis of the observations that they are the earliest genes expressed in the infected cell and that the transcription, processing, accumulation of the mrna's in the infected cell cytoplasm can take place in the presence of inhibitors of protein synthesis, such as cycloheximide. in these studies, we translated in vitro the viral mrna's extracted from cells infected maintained in the presence of cycloheximide, emetine, or anis ...	1981	6270385
differentiation of respiratory and abortigenic isolates of equine herpesvirus 1 by restriction endonucleases.	viruses classified by immunologic criteria as equine herpesvirus 1 cause respiratory disease and abortion in horses. restriction endonuclease analyses of the dna's of viruses from animals with respiratory disease and from aborted fetuses show that the patterns for respiratory viruses, while similar to each other, are entirely different from the patterns for fetal viruses. it is therefore proposed that the dna restriction endonuclease patterns of fetal and respiratory viruses analyzed in this stu ...	1981	6270790
[serial infections with two herpesviruses after renal transplantation: virological and ultrastructural features (author's transl)].	a fatal case of active viral infection in a transplant patient is reported. on the second month post transplantation, cytomegalovirus (cmv) infection occurred. it was attested by clinical and radiological symptoms, transient increase of transaminases and seroconversion with cmv antigen. patient apparently recovered, but on the third month mucocutaneous herpesvirus hominis (hvh) infection started. it was rapidly complicated by hepatitis and encephalitis which are directly responsible for the fata ...	1981	6270806
structure of the genome of equine herpesvirus type 1.		1981	6270904
herpesvirus infection enhances cholesterol and cholesteryl ester accumulation in cultured arterial smooth muscle cells.	in our previous experiments, atherosclerosis similar to that in humans was reproducibly induced in both normocholesterolemic and hypercholesterolemic specific-pathogen-free (spf) chickens by infection with marek's disease herpesvirus (mdv). in contrast, uninfected chickens fed either relatively cholesterol-poor or cholesterol-supplemented diets did not develop this arterial disease. in experiments reported here, the hypothesis that infection of arterial smooth muscle cells (smcs) with mdv would ...	1981	6271018
selective inhibition of herpesvirus deoxyribonucleic acid synthesis by acycloguanosine, 2'-fluoro-5-iodo-aracytosine, and (e)-5-(2-bromovinyl)-2'-deoxyuridine.	the selectivity of inhibition of herpesvirus deoxyribonucleic acid synthesis by acycloguanosine, 2'-fluoro-5-iodo-aracytosine, and (e)-5-(2-bromovinyl)-2'-deoxyuridine was determined by isopycnic banding of (32)p-labeled deoxyribonucleic acid from herpesvirus-infected and uninfected cells.	1981	6271053
stabilization of isometric dna viruses against thermoinactivation by lowered ionic strength.	the thermostability of isometric dna viruses increases, if the ionic strength is diminished before heating. when unpurified virus material from cell cultures is heated under conditions (temperature, time) which lead to a reduction in infectivity by 3 to 4 log10, this loss in titer is 1 to 3 log10 less, if the ionic strength is decreased by diluting the material in dist. water (1:100) before heating. a dilution in eagle's mem (1:100) or a previous dialysis against water does not have the same eff ...	1981	6271099
herpesvirus tamarinus and its relation to herpes simplex virus.	we have characterized herpesvirus tamarinus (ht), a virus of new world primates related to herpes simplex virus. ht virion dna cross-hybridized appreciably with herpes simplex virus type 1 (hsv-1) dna. the ht-hsv-1 cross-hybridizing dna sequences were distributed over the length of the genome; some dna sequences, however, were apparently more homologous than others. ht virion dna was estimated from the sum of restriction endonuclease fragments to have a mol. wt. 98 x 10(6). only 1 molar fragment ...	1981	6271910
retinoid-induced growth inhibition of herpesvirus-transformed marmoset lymphoblastoid cell lines.	the in vitro proliferation of herpesvirus-transformed marmoset lymphoblastoid cell lines (lcl) was inhibited by retinoic acid and retinyl acetate. both herpesvirus-ateles-and herpesvirus-saimiri-transformed lcl with t-cell characteristics and epstein-barr-transformed lcl with b-cell markers were more sensitive to retinoic acid than to retinyl acetate. inhibition of lcl proliferation was dependent on retinoid concentration and became apparent only after 3-4 days of exposure. in vitro assays for m ...	1981	6272171
activity of trifluorothymidine against cytomegalovirus.	trifluorothymidine (tft) was tested for antiviral activity against mouse cytomegalovirus (mcmv) and human cytomegalovirus (hcmv) in one-step replication assays. the tft concentration required to reduce virus yield by 50% (id50) was 0.22 microm for mcmv and 0.012 microm for hcmv. the antiviral activity of tft against mcmv was reversed by addition of equimolar thymidine, and no antiviral activity was demonstrable in a host cell line lacking thymidine kinase. thus, tft's anti-mcmv activity is depen ...	1981	6272627
replication of herpesvirus dna.		1981	6273071
herpesvirus antigens as markers for cervical cancer.		1981	6273263
"the herpesvirus hypothesis"--are koch's postulates satisfied?		1981	6273266
spontaneous and induced herpesvirus genome expression in marek's disease tumor cell lines.	we incubated 31 newly established marek's disease tumor cell lines at 41 degrees c for 48 h after subculturing and then examined them to determine the spontaneous rates of expression of viral internal antigen(s), viral membrane antigen(s), and virus isolation. all but two of the lines were isolated from tumors induced by clone-purified marek's disease virus strain jm-10, ga-5, rb-1b, and bc-1a in nine different genetic strains of chickens with defined histocompatibility antigens. the line-to-lin ...	1981	6273319
infection of the central nervous system of horses with equine herpesvirus serotype 1.	during the last 2 years different equine herpesviruses serotype 1 strains have been isolated from cases of paretic or paralytic disease among horses in the federal republic of germany. in this paper the available information is collated and briefly reviewed. a short description of the symptoms and the possible mechanism of the pathogenesis are given.	1981	6273556
cytomegalovirus infection of the prostate in the dasyurid marsupials, phascogale tapoatafa and antechinus stuartii.	infection by a herpesvirus producing cytomegalic disease in the prostate was demonstrated in the dasyurid marsupials, phascogale tapoatafa and antechinus stuartii. the prevalence of lesions among the latter was highest in mature animals during breeding, when the animals are known to be under stress, and in animals treated daily with high levels of exogenous corticosteroid. occasional cytomegaly was observed in the kidneys of some a. stuartii and may represent a site of latent infection. virus pa ...	1981	6273599
parma wallaby herpesvirus infection.	three parma wallabies (macropus parma) were inoculated with a herpesvirus recovered from a captive parma wallaby with fatal naturally-occurring disease. two intravenously inoculated animals died after 5 days and one animal infected via the conjunctiva and nasal mucosa was killed when moribund at 7 days. an additional two wallabies held in contact with the others became infected; they were killed at 11 days, when one was severely affected and one was mildly affected. all had small vesicles and ul ...	1981	6273601
herpesvirus-induced antigens in squamous cell carcinoma in situ of the vulva.		1981	6273722
[experiences with intravenously administered acyclovir in severe herpesvirus infections].		1981	6273756
molecular cloning of equine herpesvirus type 1 dna: analysis of standard and defective viral genomes and viral sequences in oncogenically transformed cells.	genomic dna sequences of equine herpesvirus type 1 (ehv-1) have been cloned as bamhi and ecori restriction fragments into the plasmid pbr322 and propagated in escherichia coli. with the exception of two ecori restriction fragments that reside in the s region of the viral genome, all of the cloned fragments demonstrated the same electrophoretic mobilities, restriction cleavage sites, and blot-hybridization patterns as did the parent fragments produced by bamhi or ecori digestion of virion dna. th ...	1981	6273883
episomal and nonepisomal herpesvirus dna in lymphoid tumor cell lines.	tumor cell lines derived from herpesvirus saimiri (h. saimiri)- and herpesvirus ateles (h. ateles)-induced lymphomas of new world primates and rabbits contain multiple copies of viral genomes. partial denaturation mapping and blot hybridizations of episomal dna from lymphoid tumor cell line no. 1670 showed that a 12.5md-fragment is missing which represents the ecori d- and h-fragments of virion l-dna. however, the missing piece can be demonstrated in total cellular dna by reassociation kinetics, ...	1981	6274746
effects of canavanine treatment on herpesvirus morphogenesis in cultured cells.	l-canavanine, a naturally occurring analog of arginine, effectively inhibited the morphogenesis of herpes simplex virus (hsv) and human cytomegalovirus (hcmv) when added at the time of infection, but allowed the expression of several cytopathic changes. exposure to canavanine at progressively later times ultimately led to qualitatively normal virion maturation. under no conditions were morphologically aberrant viral particles observed. however, hsv-infected cells treated at 3 h postinfection or ...	1981	6282774
antibody pattern to human cytomegalovirus in patients with adenocarcinoma of the colon.	the role of human cytomegalovirus (cmv) was examined according to serological patterns in 37 patients with adenocarcinoma of the colon (acc). the sera were examined for the presence of igg antibodies by the immunoperoxidase antibody to membrane antigens (ipama) method and by the complement-fixation (cf) test. antibody determinations were also performed by the ipama method for three other members of the herpesvirus group: epstein-barr virus (ebv), herpes simplex virus (hsv) and varicella-zoster ...	1981	6282775
serologic and molecular comparisons of several equine herpesvirus type 1 strains.	the molecular and serologic relatedness of 2 recent respiratory tract isolates of equine herpesvirus type 1, designated t1 and t2, were compared with the army 183, kentucky-a hamster-adapted (kya-ha), and l-m cell-adapted (kya-lm) strains. electrophoresis in polyacrylamide gels revealed differences in virion structural proteins among 4 purified strains. seven envelope glycoproteins (molecular weight of 93,000, 65,000, 62,000, 60,000, 36,000, 20,000, and 18,000) corresponding to virion proteins 1 ...	1981	6280520
a simple in vitro differentiation between turkey herpesvirus and marek's disease virus.	the growth and plaque formation by turkey herpesvirus (hvt) amd marek's disease herpesvirus (mdhv) were examined in qt35 cells, a continuous fibroblast cell line derived from chemically induced tumors of japanese quail. hvt grew and formed plaques consistently in qt35 cells when inoculated with cell-culture-propagated virus or peripheral mononuclear leukocytes (pml) from chickens that had been inoculated with hvt. both oncogenic and nononcogenic strains of mdhv, however, failed to grow and induc ...	1981	6279072
natural killer cell activity in chickens exposed to marek's disease virus: inhibition of activity in susceptible chickens and enhancement of activity in resistant and vaccinated chickens.	chickens of 2 genetic lines (lines p and n) were inoculated with a pathogenic strain of marek's disease (md) virus (mdv) and chronologically examined for disease response and natural killer (nk) cell expression. the nk cell reactivity was assayed in an in vitro cytotoxicity assay in which effector cells from the spleen of test chickens were reacted with 51cr-labeled lscc-rp9 target cells. chickens of line p developed progressive debilitating disease and a high incidence of gross tumors and death ...	1981	6279075
infection of sheep and goats with bovid herpesvirus 2.	sheep and goats were shown to be susceptible to experimental infection with bovid herpesvirus 2 (bhv2), administered by either the intradermal or intravenous route. lesions developing in sheep following intradermal inoculation of virus were similar to those in cattle inoculated intradermally, whereas the lesions in goats resolved without ulceration or scabbing. disseminated circumscribed skin lesions developed in sheep and goats given bhv2 by the intravenous route. these lesions resolved in four ...	1981	6281868
collinear relationships of herpesvirus papio dna to epstein--barr virus dna.		1981	6259833
the relationship of herpesvirus to carcinoma of the prostate.	a laboratory based epidemiologic study was done to determine the possible relationship of herpesvirus type 2 to carcinoma of the prostate. a total of 305 patients (224 with benign prostatic hypertrophy and 81 with prostatic carcinoma) who underwent transurethral resection of the prostate was studied. viral cultures were obtained from urethral swabs and urine preoperatively. prostatic tissue removed at operation was grown as tissue explants, using a monolayer cell culture technique, and examined ...	1981	6259379
latent infections with marek's disease virus and turkey herpesvirus.	the number of plaque-forming units (pfu) from lymphocytes latently infected with oncogenic (ga-5) or nononcogenic (sb-1) marek's disease virus (mdv) was decreased after complement lysis with antisera against thymus-derived lymphocytes or bursa-derived lymphocytes; an additive effect with dual treatment was observed. complement lysis with antisera against marek's disease tumor-associated surface antigen (putative tumor antigen) had little effect, which suggests an absence of the antigen on latent ...	1981	6259403
tumorigenicity of herpesvirus-transformed cells correlates with production of plasminogen activator.	our studies first demonstrated that established hamster cell lines transformed in vitro by herpesviruses activate plasminogen more effectively than normal hamster fibroblasts. this ability is probably due to increased levels of the enzyme plasminogen activator (pa). in the studies described here, the 333-8-9 cell line, originally transformed by herpes simplex virus type 2 strain 333, was used to derive subclonal lines that maintained stable pa phenotypes over the course of long in vitro passage. ...	1981	6100964
viral-induced leukocyte interferon in vesicle fluid from lesions of recurrent herpes labialis.	antiviral activity characterized as human interferon (huifn) was demonstrated in vesicle fluid from lesions of recurrent herpes labialis in 18 of 19 otherwise healthy patients. high titers (geometric mean, 29,200 units) were present during the early course of lesion development. antiviral activity in vesicle fluid was neutralized by antibody to leukocyte (types i) huifn (huifn-alpha) in the eight patients in whom it was tested. these results indicate that huifn-alpha is present in the local lesi ...	1981	6165784
antibodies to herpesvirus hominis types 1 and 2 in malnourished nigerian children.	antibodies to herpesvirus hominis (hvh) types 1 and 2 were determined by a micro-neutralisation method in 37 children with kwashiorkor, 16 with marasmus, and in 64 well-nourished control children. all the children were aged between 1 and 4 years. the prevalence of antibodies was similar in the two sexes and at different ages. hvh-1 antibodies were present in 51% of children with kwashiorkor, in 44% with marasmus, and in 26% of well-nourished children, reflecting the very poor socioeconomic condi ...	1981	6258486
epstein-barr virus and herpesvirus saimiri: sensitivity to interferons and interferon inducers.	the in vitro sensitivity of oncogenic herpesviruses, epstein-barr virus (ebv), and herpesvirus saimiri (hvs) to human interferon produced by normal human leukocytes (le), lymphoblastoid cell lines (lyi), and diploid fibroblasts (fi) was studied. four virus strains were used: hvs s295c, the highly oncogenic hvs s396-o, the transforming b95-8 strain of ebv, and the nontransforming p3hr1 strain of ebv. all interferons were active when applied to the cells after absorption of hvs and p3hr1-ebv, alth ...	1981	6165852