Publications

oligomerization of hantavirus nucleocapsid protein: analysis of the n-terminal coiled-coil domain.	hantaviruses constitute a genus in the family bunyaviridae. they are enveloped negative-strand rna viruses with a tripartite genome encoding the nucleocapsid (n) protein, the two surface glycoproteins gn and gc, and an rna-dependent rna polymerase. the n protein is the most abundant component of the virion; it encapsidates genomic rna segments forming ribonucleoproteins and participates in genome transcription and replication as well as virus assembly. in the course of rna encapsidation, n prote ...	2006	16940519
diagnosis and treatment of new world hantavirus infections.	the purpose of this review is to summarize the current knowledge regarding the diagnosis and treatment of indigenous new world hantavirus infections.	2006	16940866
tropical infections in the icu.	certain arthropod-borne infections are common in tropical regions because of favorable climatic conditions. water-borne infections like leptospirosis are common due to contamination of water especially during the monsoon floods. infections like malaria, leptospirosis, dengue fever and typhus sometimes cause life threatening organ dysfunction and have several overlapping features. most patients present with classicial clinical syndromes: fever and thrombocytopenia are common in dengue, malaria an ...	2006	16944613
characterization of shock in a hamster model of hantavirus infection.	human hantavirus cardiopulmonary syndrome (hcps) due to andes, sin nombre and other hantaviruses is characterized by severe pulmonary capillary leak and cardiogenic shock. hamsters, the only animal manifesting hcps-like disease, were instrumented with radiotelemeters that enabled ambulatory intracarotid blood pressure recording within an animal biosafety level-4 facility. following infection with andes virus, blood pressure and heart rate decreased slowly in a biphasic manner during the first 7 ...	2006	16950493
genetic analysis of thailand hantavirus in bandicota indica trapped in thailand.	sixty one tissue samples from several rodent species trapped in five provinces of thailand were examined for the presence of hantaviral markers by enzyme-immunoassay and immunoblotting. four samples, all from the great bandicoot rat bandicota indica, were confirmed positive for the hantaviral n-antigen. two of them were trapped in nakhon pathom province, the other two in nakhon ratchasima province, approximately 250 km from the other trapping site. when analysed by rt-nested pcr, all four rodent ...	2006	16953877
nitric oxide and peroxynitrite have different antiviral effects against hantavirus replication and free mature virions.	reactive nitrogen intermediates (rni), like nitric oxide (no) and peroxynitrite, have antiviral effects against certain viruses. hantaviruses, like other members of the bunyaviridae family, have previously not been shown to be sensitive to rni. in this study, we compared the effects of no and peroxynitrite on hantavirus replication and free mature virions in vitro, and of inducible nitric oxide synthase (inos) in hantavirus-infected suckling mice. the no-generating compound s-nitroso-n-acetylpen ...	2006	16955520
incubation period of hantavirus cardiopulmonary syndrome.	the potential incubation period from exposure to onset of symptoms was 7-39 days (median 18 days) in 20 patients with a defined period of exposure to andes virus in a high-risk area. this period was 14-32 days (median 18 days) in 11 patients with exposure for <48 hours.	2006	16965713
hantavirus n protein exhibits genus-specific recognition of the viral rna panhandle.	a key genomic characteristic that helps define hantavirus as a genus of the family bunyaviridae is the presence of distinctive terminal complementary nucleotides that promote the folding of the viral genomic segments into "panhandle" hairpin structures. the hantavirus nucleocapsid protein (n protein), which is encoded by the smallest of the three negative-sense genomic rna segments, undergoes in vivo and in vitro trimerization. trimeric hantavirus n protein specifically recognizes the panhandle ...	2006	16971445
regions of importance for interaction of puumala virus nucleocapsid subunits.	puumala virus (puuv) is a hantavirus that causes a mild form of hemorrhagic fever with renal syndrome in northern and central europe, and in large parts of russia. the nucleocapsid (n) protein encoded by hantaviruses plays an important role in the life-cycle of these viruses, and one important function for the n-protein is to oligomerize, surround and protect the viral rnas. we have identified amino- and carboxy-terminal regions involved in puuv n-n interactions, which comprise amino acids 100-1 ...	2006	16972031
characterization of a new puumala virus genotype associated with hemorrhagic fever with renal syndrome.	hantavirus serotype puumala (puuv) is the etiologic agent of nephropathia epidemica (ne), a mild variant of hemorrhagic fever with renal syndrome (hfrs) in europe, with lethality rates up to 1% among human patients. the serotype puuv is composed of numerous geographically restricted strains forming a great number of phylogeographic lineages, sublineages, variants, and clusters. we describe a new, geographically and phylogenetically well-defined pathogenic puuv sublineage in southwest germany (st ...	2006	16972039
new world hantavirus in humans, french guiana.		2006	16972355
the pathogenic ny-1 hantavirus g1 cytoplasmic tail inhibits rig-i- and tbk-1-directed interferon responses.	hantaviruses cause two diseases with prominent vascular permeability defects, hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. all hantaviruses infect human endothelial cells, although it is unclear what differentiates pathogenic from nonpathogenic hantaviruses. we observed dramatic differences in interferon-specific transcriptional responses between pathogenic and nonpathogenic hantaviruses at 1 day postinfection, suggesting that hantavirus pathogenesis may in part be de ...	2006	16973572
[the new-world hantaviruses. ecology and epidemiology of an emerging virus in latin america].	the hantaviruses are a group of emerging rodent-borne pathogens (family bunyaviridae; genus hantavirus) that are etiologic agents for hemorrhagic fever with renal syndrome (hfrs) in europe and asia and hantavirus cardiopulmonary syndrome (hcps) in the americas. hfrs is associated with rodents of the family muridae, subfamilies murinae and arvicolinae; hps is associated with rodents of the subfamily sigmodontinae. since the identification of hcps in usa in 1993, a large number of cases of hps and ...	2006	16977974
tick-borne encephalitis virus in clethrionomys glareolus in the czech republic.	a total of 474 specimens from 157 rodents caught at the military training area of boletice in the south of the czech republic from may to november 1999 were screened for tbev by nested pcr. tbev-specific rna was amplified from lung, kidney, and spleen derived from one clethrionomys glareolus in the first rt-pcr round. sequence analysis revealed a 100% identity to the tbev strain neudoerfl. tbev presence in the sample was confirmed by mouse brain passage of backup samples and cell culture. the re ...	2006	17187572
developing global climate anomalies suggest potential disease risks for 2006-2007.	el niño/southern oscillation (enso) related climate anomalies have been shown to have an impact on infectious disease outbreaks. the climate prediction center of the national oceanic and atmospheric administration (noaa/cpc) has recently issued an unscheduled el niño advisory, indicating that warmer than normal sea surface temperatures across the equatorial eastern pacific may have pronounced impacts on global tropical precipitation patterns extending into the northern hemisphere particularly ov ...	2006	17194307
implication of phylogenetic systematics of rodent-borne hantaviruses allows understanding of their distribution.	hantaviruses' distribution is reassessed after performing a cladistic analysis on 93 strains isolated from rodents, and one used as outgroup: thottapalayam isolated from a shrew. while most hantaviruses found in wild animals were collected in northern asia, europe, north america, and south america, only thottapalayam and thailand were found in south and southeastern asia. thottapalayam is highly divergent from the other known hantaviruses and may represent the emerging tip of a different lineage ...	2006	17135493
[emergence of new pneumonia: besides severe acute respiratory syndrome].	important epidemiological modifications have been registered in respiratory infections, both in immunocompetent and immunocompromised hosts. pathogens with modified antibiotic susceptibility patterns have emerged, which display an increased antibiotic resistance, such as s. pneumoniae, s. aureus, h. influenzae. this trait has a strong impact on the therapeutic choices, particularly when an empiric antibiotic treatment is selected. the prevalence of bacterial species showing non-susceptibility to ...	2006	17146421
[isolation and characterization of hantavirus carried by rodents in huludao, liaoning province].	to investigate the hantavirus infection and their genotype in rodents in huludao.	2006	17152513
[study on the coinfection of hantavirus and orientia tsutsugamushi in tissue cell culture].	to investigate the possibility of hantavirus (hv) and orientia tsutsugamushi (ot) coinfection in their hosts.	2006	17152514
phylogenetic and geographical relationships of hantavirus strains in eastern and western paraguay.	recently, we reported the discovery of several potential rodent reservoirs of hantaviruses in western (holochilus chacarius) and eastern paraguay (akodon montensis, oligoryzomys chacoensis, and o. nigripes). comparisons of the hantavirus s- and m-segments amplified from these four rodents revealed significant differences from each another and from other south american hantaviruses. the alp strain from the semiarid chaco ecoregion clustered with leguna negra and rio mamore (ln/rm), whereas the bm ...	2006	17172380
use of igg avidity to indirectly monitor epizootic transmission of sin nombre virus in deer mice (peromyscus maniculatus).	an igg avidity assay was developed to differentiate deer mice that had recently acquired sin nombre virus (snv) from those that were infected in the distant past. using this procedure, low avidity antibodies were predominantly detected in experimentally infected deer mice (89.5%) within the first 30 days post-inoculation. the assay was then applied to sera from naturally infected deer mice collected during a field investigation associated with a cluster of hantavirus pulmonary syndrome cases. a ...	2006	17172381
[hantavirus in zulia state?].	during the last years, there has been a worldwide rise in the number of infectious diseases caused by newly discovered agents, in some cases and in others, by variants known as emergent or reemergent pathogens. the analysis of the nucleotide sequences of four rna-positive hantaviruses (s. altoni) suggests that the caño delgadito virus is widely distributed in the venezuelan plains. a similar distribution has been found for some isolates and strains of a novel hantavirus (proposed name "mamporal" ...	2006	17176900
evaluation of promed-mail as an electronic early warning system for emerging animal diseases: 1996 to 2004.	to identify emerging animal and zoonotic diseases and associated geographic distribution, disease agents, animal hosts, and seasonality of reporting in the program for monitoring emerging diseases (promed)-mail electronic early warning system.	2006	17014355
maternal antibodies postpone hantavirus infection and enhance individual breeding success.	the transfer of maternal antibodies from mother to progeny is a well-known phenomenon in avian and mammalian species. optimally, they protect the newborn against the pathogens in the environment. the effect of maternal antibodies on microparasite transmission dynamics may have important consequences for both the fitness of the host and the epizootic processes of the pathogens. however, there is a scarcity of studies examining these effects in free-living wild species. we studied the influence of ...	2006	17015326
development of novel immunoglobulin g (igg), iga, and igm enzyme immunoassays based on recombinant puumala and dobrava hantavirus nucleocapsid proteins.	human infections with asian and european hantaviruses can result in hemorrhagic fever with renal syndromes of differing severities characterized by renal dysfunction and sometimes by pulmonary symptoms. for the serological detection of human infections by hantaviruses relevant for europe, we developed monoclonal antibody capture immunoglobulin g (igg) and iga enzyme-linked immunosorbent assays (elisas) based on yeast-expressed nucleocapsid proteins of puumala and dobrava hantaviruses. moreover, ...	2006	17021245
mild course of puumala nephropathy in children in an area with sporadic occurrence hantavirus infection.	the first three children with puumala virus nephropathy diagnosis in the czech republic are reported on. a boy and two girls were admitted with symptoms of interstitial nephritis. the medical history in all children revealed flu-like symptoms. all patients were mildly pyrexial and had elevated erythrocytes sedimentation rate, c-reactive protein and low hemoglobin levels. serum creatinine levels were elevated and proteinuria exceeded 700 mg/l in all children. tubular proteinuria, glycosuria, high ...	2006	17024393
dynamics of puumala virus infection in bank voles in ardennes department (france).	the hantaviruses (genus hantavirus, family bunyaviridae) include human pathogens and occur worldwide. in western and central europe, the predominant serotype is puumala (puu) virus, which causes epidemic nephropathy. voles are considered to be the main reservoir and the vector of puu virus. a total of 719 rodents (mainly clethrionomys glareolus, apodemus sp.) trapped by capture-mark-recapture (cmr) in four sites in ardennes department (france) between april 2004 and october 2005 were tested for ...	2006	17027178
sin nombre viral rna load in patients with hantavirus cardiopulmonary syndrome.	to address the role that viral load plays in pathogenesis in patients with hantavirus cardiopulmonary syndrome (hcps), we quantified sin nombre virus s segment viral rna in plasma samples from 27 acutely ill patients. for 6 patients, we examined viral load in matched plasma, urine, and/or tracheal aspirate throughout the time when the patients were in intensive care. peak titers in plasma reached 1.8 x 106 copies/ml; none of the patients had viral rna in urine. titers in tracheal aspirates did n ...	2006	17054070
update on emerging infections from the centers for disease control and prevention. hantavirus pulmonary syndrome--five states, 2006.		2006	17061318
[epizootology of hemorrhagic fever with renal syndrome in the central chernozem region].	a total of 5149 small mammals belonging to 16 species were collected from the lipetsk, voronezh, and belgorod regions (40 administrative districts) in 2003-2004 and examined by elisa and ifa to detect hantavirus antigen and antibodies in the lung tissues. hantavirus circulation was revealed in 13 species, the highest hantavirus activity being detected in field (apodemus agrarius) and small wood (a. (s) uralensis) mice (dobrava-belgrad virus), bank (clethrionomis glareolus) (puumala virus) and co ...	2006	17087062
serological survey for viral pathogens in turkish rodents.	wild rodents (n = 330) were trapped around the villages of altindere and coşandere (maçka, trabzon province), ayder, ortan, and yolkiyi (camlihemşin, rize province), and bozdag (odemiş, izmir province) in northeastern and western turkey during april 2004. samples were tested for arenavirus, hantavirus, and cowpox virus (family poxviridae, genus orthopoxvirus, cpxv) antibodies by using immunofluorescence assays (ifas). antibodies against arenaviruses were found in eight of 330 (2.4%) rodents. are ...	2006	17092901
emergency medicine and the public's health: emerging infectious diseases.	in recent years, multiple global forces have contributed to the emergence and widespread distribution of previously unknown disease entities. this article discusses ebola virus, west nile virus, and hantavirus as representative emerging infectious diseases. smallpox is discussed along with concerns about the safety of the smallpox vaccine, given the uncertain risk of bioterrorism and smallpox exposure. ed physicians must become familiar with the presentation, management, and public health impact ...	2006	16982350
tumor necrosis factor-alpha genetic predisposing factors can influence clinical severity in nephropathia epidemica.	severe human infection with hantavirus is characterized by high fever, cold chills, thrombocytopenia, arterial hypotension, acute renal failure, and/or adult respiratory distress syndrome (ards)-like pulmonary involvement, but the clinical course varies greatly between individuals. we investigated whether genetically determined differences in tumor necrosis factor (tnf)-alpha production can influence the severity of hantavirus disease. we studied a tnf-alpha single-nucleotide promoter polymorphi ...	2006	16987073
geographical distribution of hantaviruses in thailand and potential human health significance of thailand virus.	phylogenetic investigations, sequence comparisons, and antigenic cross-reactivity studies confirmed the classification of thailand virus (thaiv) as a distinct hantavirus species. the examination of sera from 402 rodents trapped in 19 provinces of thailand revealed that five greater bandicoot rats (bandicota indica) and one lesser bandicoot rat (b. savilei) from four provinces were focus reduction neutralization test (frnt) antibody-positive for thaiv. one of 260 patients from surin province in t ...	2006	17124002
catacamas virus, a hantaviral species naturally associated with oryzomys couesi (coues' oryzomys) in honduras.	the primary objective of this study was to extend our knowledge of the geographical distribution, genetic diversity, and natural host associations of the hantaviruses indigenous to north america. antibody to a hantavirus was found in 5 (20.8%) of 24 coues' oryzomys (oryzomys couesi) and none of 41 other rodents captured near the town of catacamas in eastern honduras, and a hantavirus was isolated from one of the antibody-positive coues' oryzomys. analyses of nucleotide and amino acid sequence da ...	2006	17124003
[surveillance on natural infection of rodents with hantavirus in shenzhen city and identification of a hantavirus strain sz2083].	for clarifying the situation of the natural infection of rodents having hemorrhagic fever with renal syndrome (hfrs) virus and to type hantavirus (hv) using molecular technique in shenzhen city in 2005, and offering guidance for prevention and control of hfrs.	2006	17402203
deer mouse movements in peridomestic and sylvan settings in relation to sin nombre virus antibody prevalence.	prevalence of antibody to sin nombre virus (snv) has been found to be nearly twice as high in deer mice (peromyscus maniculatus) in peridomestic settings as in sylvan settings in two studies in montana and one in new mexico. we investigated whether this difference may be related to a difference in deer mouse movements in the two settings. we used radiotelemetry to determine home range size and length of movement for 22 sylvan (1991-1992) and 40 peridomestic deer mice (1995-1999). we also determi ...	2006	17255448
changes in sin nombre virus antibody prevalence in deer mice across seasons: the interaction between habitat, sex, and infection in deer mice.	we examined the impact of season and habitat on sin nombre virus (snv) seroprevalence in deer mice (peromyscus maniculatus) in utah's great basin desert from may 2002 through summer 2003. low mouse captures in 2002 limited analysis for that year. in two seasons during 2003, mouse density and sagebrush cover were positively linked (spring: r = 0.8, p = 0.01; summer: r = 0.8, p = 0.04). in the spring, seroprevalence was negatively correlated with density (r = -0.9, p< 0.01); male and female antibo ...	2006	17255449
[progress on the study of biological characteristic of hantavirus].		2006	17299972
[haemorrhagic fever with renal syndrome (hfrs)--hantavirus infection disease appearing in poland].	authors take up the problem of occurrence of the haemorrhagic fever with renal syndrome in poland and present the measures undertaken by chief sanitary inspectorate, which brought to detection of the first, serologically confirmed case of hfrs in may 2005. they strongly emphasize the existence of the disease on polish territory despite the poor epidemiological recognition, an possibility of its testing.	2006	17345831
[a case of haemorrhagic fever with renal syndrome (hfrs)].	in our article we describe clinical and epidemiological analysis of the first case of haemorrhagic fever with renal syndrome (hfrs)--with serological confirmation--in poland.	2006	17345843
wildlife, environment and (re)-emerging zoonoses, with special reference to sylvatic tick-borne zoonoses in north-western italy.	over the last century, changes in land-use, modification of agriculture-livestock production systems, disruption of wildlife habitats, increase of human activities, higher frequency of international and intercontinental travels, wider circulation of animals and animal products have contributed to alter the distribution, presence and density of hosts and vectors. as a result, the number of emerging and reemerging diseases, including zoonoses, have greatly increased. some infectious pathogens, ori ...	2006	17361062
social status does not predict responses to seoul virus infection or reproductive success among male norway rats.	trade-offs exist among life history strategies that are used to increase survival and reproduction; such that, males that engage in more competitive behaviors may be more susceptible to infection. hantaviruses are transmitted horizontally between rodents through the passage of virus in saliva during wounding and male rodents are more likely to be infected with hantaviruses than females. to determine whether a trade-off exists between dominance and susceptibility to seoul virus infection, male lo ...	2006	16040226
neutralizing antibodies in survivors of sin nombre and andes hantavirus infection.	we evaluated titers of homotypic and heterotypic neutralizing antibodies (nabs) to andes and sin nombre hantaviruses in plasma samples from 20 patients from chile and the united states. all but 1 patient had high titers of nab. none of the plasma samples showed high titers against the heterologous virus.	2006	16494739
a novel method for cloning of non-cytolytic viruses.	hantaviruses are rodent-borne pathogens with a segmented single-stranded rna genome of negative polarity. spontaneous occurrence of variants with genetic heterogeneity have been observed both in vivo and in vitro. the objective of this study was to establish a method for the cloning of genetically homogenous hantaviruses which can be used for subsequent functional studies. infected veroe6 cells were incubated with an agarose/medium overlay to prevent uncontrolled distribution of de novo synthesi ...	2006	16504312
microbiological contamination of laboratory mice and rats in korea from 1999 to 2003.	to survey the microbiological contamination of laboratory mice and rats in korea during a 5-year period, we monitored animals housed in mouse and rat facilities with either barrier or conventional systems. at barrier and conventional mouse facilities, the most important pathogen identified was mouse hepatitis virus (mhv), while mycoplasma pulmonis was the most important pathogen at conventional rat facilities. interestingly, hantavirus was recovered from both barrier and conventional mouse facil ...	2006	16508207
production of hantavirus puumala nucleocapsid protein in saccharomyces cerevisiae for vaccine and diagnostics.	the production of hantavirus puumala nucleocapsid (n) protein for potential applications as a vaccine and for diagnostic purposes was investigated with saccharomyces cerevisiae as a recombinant host. the n protein gene and the hexahistidine tagged n (h-n) protein gene were expressed intracellular from a 2-microm plasmid vectors under the control of a fused galactose inducible gal10-pyk promoter. for monitoring the recombinant gene expression, a h-n and a gfp fusion protein was used. different cu ...	2006	16513199
dobrava, but not saaremaa, hantavirus is lethal and induces nitric oxide production in suckling mice.	hantaviruses are the causative agents of hfrs and hcps (hemorrhagic fever with renal syndrome and hantavirus cardiopulmonary syndrome), two severe, and often fatal human diseases. mortality from hfrs varies between hantaviruses; hantaan and dobrava show the highest, seoul intermediate, and puumala low mortality. saaremaa, genetically closely related to dobrava, is also known to induce hfrs, with low or no mortality. in this study, mice were inoculated with dobrava and saaremaa viruses to test fo ...	2006	16513381
generation of monoclonal antibodies of desired specificity using chimeric polyomavirus-derived virus-like particles.	foreign protein sequences presented on hamster polyomavirus (hapyv) major capsid protein vp1-derived virus-like particles (vlps) have been demonstrated to be highly immunogenic. the current study was aimed to evaluate vp1-derived chimeric vlps as tools for hybridoma technology to generate monoclonal antibodies (mabs) of desired specificity. chimeric vlps containing inserts of different size and origin were used as immunogens. chimeric vlps carrying a 9 amino acid (aa)-long cytotoxic t-cell epito ...	2006	16516908
corticosteroids combined with continuous veno-venous hemodiafiltration for treatment of hantavirus pulmonary syndrome caused by puumala virus infection.	reported here are two cases of hantavirus pulmonary syndrome caused by puumala virus infection, which rapidly resolved after initiation of corticosteroid treatment combined with continuous veno-venous hemodiafiltration. these cases emphasize the role of the inflammatory response in the pathogenesis of hantavirus pulmonary syndrome.	2006	16550348
is there an association of pneumocystis infection with the presence of arena-, hanta-, and poxvirus antibodies in wild mice and shrews in finland?	as part of studies on the nature of the endemic virus infections in natural rodent hosts, the possible association of cyst forms of pneumocystis spp. with the presence of hanta-, cowpox-, and arenavirus antibodies in wild mice (apodemus flavicollis, n=105; apodemus agrarius, n=63; micromys minutus, n=50) and the common shrew (sorex araneus, n=101) was studied in south-central finland. one hantavirus (saaremaa virus, saav) seropositive a. agrarius, and 2 cowpoxvirus (cpxv) seropositive s. araneus ...	2006	16556344
which hantaviruses in india ?		2006	16567875
maternally derived recombinant human anti-hantavirus monoclonal antibodies are transferred to mouse offspring during lactation and neutralize virus in vitro.	transgenic mice expressing a recombinant human monoclonal antibody (rhmab) against hantavirus were generated. these mice could be used as models to explore the possibilities of producing rhmabs for therapeutic purposes. the highest concentration of the rhmab in the milk of the transgenic females was 6.6 mg/ml. the rhmab was also detected in the sera of pups fed by the transgenic females. both the rhmabs in the milk of transgenic mice and those in the sera of suckling pups were found to be active ...	2006	16571835
saaremaa hantavirus should not be confused with its dangerous relative, dobrava virus.		2006	16597913
biological control agents elevate hantavirus by subsidizing deer mouse populations.	biological control of exotic invasive plants using exotic insects is practiced under the assumption that biological control agents are safe if they do not directly attack non-target species. we tested this assumption by evaluating the potential for two host-specific biological control agents (urophora spp.), widely established in north america for spotted knapweed (centaurea maculosa) control, to indirectly elevate sin nombre hantavirus by providing food subsidies to populations of deer mice (pe ...	2006	16623730
ten-year prognosis of puumala hantavirus-induced acute interstitial nephritis.	nephropathia epidemica (ne) is a hemorrhagic fever with renal syndrome caused by puumala hantavirus. its long-term prognosis is considered favorable. there are, however, some reports about subsequent hypertension, glomerular hyperfiltration, and proteinuria after previous hantavirus infection. therefore, we studied 36 patients 5 and 10 years after acute ne, with 29 seronegative controls. office blood pressure, ambulatory 24-h blood pressure (abp), glomerular filtration rate (gfr), and proteinuri ...	2006	16641933
a friday afternoon case of apparent anti-glomerular basement nephritis.		2006	16644771
a new puumala hantavirus subtype in rodents associated with an outbreak of nephropathia epidemica in south-east germany in 2004.	a micro-epidemic of hantavirus infections occurred in lower bavaria, south-east germany, starting in april 2004. while only three cases were registered from 2001 to 2003, a dramatically increased number of clinically apparent human hantavirus infections (n=38) was observed in 2004, plus seven additional cases by june 2005. to determine the reservoir responsible for the infections, a total of 43 rodents were trapped in lower bavaria. serological and genetic investigations revealed that puumala vi ...	2006	16650330
[identification of infectious agents in cases suspicious of hantavirus infection but with negative serology].	the diagnosis of acute respiratory illness caused by hantavirus is based in the rapid and progressive clinical course, epidemiological background and the serological confirmation of the virus. when the presence of the virus is not confirmed a differential diagnosis must be made with other infections. between 1999 and 2001, the chilean public health institute received 1063 blood samples from patients with a suspicious clinical picture, to study the presence of andes strain of hantavirus. in 134 o ...	2006	16676106
the effect of biodiversity on the hantavirus epizootic.	we analyze a mathematical model of the epizootic of hantavirus in mice populations, including the effect of species that compete with the host. we show that the existence of the second species has an important consequence for the prevalence of the infectious agent in the host. when the two mice species survive in the ecosystem, the competitive pressure of the second species may lead to reduction or complete elimination of the prevalence of infection. the transition between the disappearance of t ...	2006	16676531
[viral hemorrhagic fevers in brazil].	to call attention to viral hemorrhagic fevers, diseases that are mostly underdivulged and, probably, underreported, we present here case reports of the 4 diseases of this kind that occur in brazil: yellow fever, dengue haemorrhagic fever/dengue shock syndrome, arenavirus haemorrhagic fever and hantavirus cardiopulmonary syndrome. relevant clinical, epidemiological and laboratorial diagnostic aspects of these viral haemorrhagic fevers are also shown here. these diseases have a high case fatality ...	2006	16699651
hantavirus in african wood mouse, guinea.	hantaviruses are rodent-borne, emerging viruses that cause life-threatening human diseases in eurasia and the americas. we detected hantavirus genome sequences in an african wood mouse (hylomyscus simus) captured in sangassou, guinea. sequence and phylogenetic analyses of the genetic material demonstrate a novel hantavirus species, which we propose to name "sangassou virus."	2006	16704849
hantaviruses in serbia and montenegro.	hantaviruses are endemic in the balkan peninsula. an outbreak of hemorrhagic fever with renal syndrome occurred in 2002 in serbia and montenegro. the epidemiologic characteristics and genetic relatedness of dobrava/belgrade virus strains responsible for most cases are described.	2006	16707066
truncated recombinant dobrava hantavirus nucleocapsid proteins induce strong, long-lasting immune responses in mice.	we describe the cloning and expression of dobrava hantavirus (dobv) nucleocapsid proteins and a truncated form consisting of the first 118 n-terminal amino acids, and the capacity of these e. coli icone 200-expressed recombinant proteins (rnp) to induce a protective immune response against dobv in mice. as an alternative carrier protein, the outer membrane protein a derived from klebsiella pneumoniae (rp40) has been coupled to different rnp constructs. all recombinant proteins were found to be h ...	2006	16714853
characterization of truncated hantavirus nucleocapsid proteins and their application for serotyping.	hemorrhagic fever with renal syndrome (hfrs) is a fulminant infectious disease characterized by fever, hemorrhage, renal impairment, and thrombocytopenia. hantaviruses associated with this belong to different serotypes: hantaan (htn), seoul (seo), dobrava/belgrade (dob), and puumala (puu). the first two, htn and seo, are endemic in china. to investigate the epidemiology of hfrs and virus transmission in china, we constructed prokaryotic plasmids encoding truncated recombinant htn and seo nucleoc ...	2006	16721853
crimean-congo haemorrhagic fever presenting as thrombotic microangiopathy and acute renal failure.		2006	16735392
[investigation on hantaviruses infection in rodents from free markets in beijing areas].	in order to find out the factors related to hemorrhagic fever with renal syndrome (hfrs) infection, and to evaluate the probability of ecdemic hantaviruses (hv) infection in rodents in beijing areas.	2006	16749997
a pseudotype vesicular stomatitis virus containing hantaan virus envelope glycoproteins g1 and g2 as an alternative to hantavirus vaccine in mice.	we examined whether a vesicular stomatitis virus (vsv) pseudotype bearing the hantavirus envelope glycoproteins (gps) g1 and g2 (vsvdeltaghtn) could be used as a safe and effective alternative to native hantavirus. mice were immunized with purified particles of vsvdeltaghtn. after the second immunization, all mice produced anti-gp antibody as detected in elisa and a neutralization test. after the third immunization, the mice were challenged with hantaan virus. neither anti-np antibody producti ...	2006	16426712
serological assays for the detection of human andes hantavirus infections based on its yeast-expressed nucleocapsid protein.	the objective of the study was to develop and evaluate igm and igg elisas and an igg western blot test for the serological detection of human infections with andes virus (andv), the major cause of hantavirus cardiopulmonary syndrome (hcps) in south america.	2006	16428893
targeting vascular injury using hantavirus-pseudotyped lentiviral vectors.	restenosis is a pathological condition involving intimal hyperplasia and negative arterial remodeling. gene therapy vectors have shown modest therapeutic effects, but the level of infectivity has been relatively poor. in the present study we have designed a modified lentiviral vector (lv) pseudotyped with a strain of hantavirus (htnv) to improve the transduction efficiency into vascular smooth muscle and endothelial cells in vitro and in vivo. in vivo studies using adult new zealand white rabbit ...	2006	16431160
bidirectional virus secretion and nonciliated cell tropism following andes virus infection of primary airway epithelial cell cultures.	hantavirus pulmonary syndrome (hps) is an acute disease resulting from infection with any one of a number of new world hantaviruses. hps has a mortality rate of 40% and, unlike many other severe respiratory diseases, often occurs in young, healthy adults. infection is usually initiated after inhalation of rodent excreta containing virus particles, but human-to-human transmission has been documented. postmortem tissue samples show high levels of viral antigen within the respiratory endothelium, b ...	2006	16414986
hantaan/andes virus dna vaccine elicits a broadly cross-reactive neutralizing antibody response in nonhuman primates.	at least four hantavirus species cause disease with prominent renal involvement-hemorrhagic fever with renal syndrome (hfrs); and several hantavirus strains cause disease with significant pulmonary involvement-hantavirus pulmonary syndrome (hps). the most prevalent and lethal hantaviruses associated with hfrs and hps are hantaan virus (htnv) and andes virus (andv), respectively. here, we constructed a dna vaccine plasmid (pwrg/ha-m) that contains both the htnv and andv m gene segments. rhesus ma ...	2006	16378630
hantavirus and arenavirus antibody prevalence in rodents and humans in trentino, northern italy.	the spatial and temporal distribution of hantavirus and arenavirus antibody-positive wild rodents in trentino, italy, was studied using immunofluorescence assays (ifa) in two long-term sites trapped in 2000-2003, and six other sites trapped in 2002. the overall hantavirus seroprevalence in the bank voles, clethrionomys glareolus (n=229) screened for puumala virus (puuv) antibodies was 0.4%, and that for apodemus flavicollis mice (n=1416) screened for dobrava virus (dobv) antibodies was 0.2%. ant ...	2006	16371172
soochong virus: an antigenically and genetically distinct hantavirus isolated from apodemus peninsulae in korea.	hantaan (htn) virus, the etiologic agent of clinically severe hemorrhagic fever with renal syndrome (hfrs), was first isolated in 1976 from lung tissue of a striped-field mouse (apodemus agrarius) captured in songnae-ri, gyeonggi province, republic of korea. found primarily in mountainous areas, the korean field mouse (a. peninsulae) is the second-most dominant field rodent species found throughout korea. a new hantavirus, designated soochong (soo), was isolated in vero e6 cells from four a. pen ...	2006	16372283
first evidence of fatal hantavirus nephropathy in india, mimicking leptospirosis.		2006	16361279
cellular entry of hantaan virus a9 strain: specific interactions with beta3 integrins and a novel 70kda protein.	cellular entry of pathogenic hantaviruses had been shown to be mediated by beta3 integrins. however, no direct evidence exists that hantavirus binds to beta3 integrins, and integrin beta3 subunit is not expressed on some cells permissive to hantavirus infection. in this report, utilizing beta3-integrin-transfected cho cells, we demonstrated that integrin beta3 subunit renders cho cells susceptible to chinese hantaan virus (htn) strain a9 (isolated in china), and the viral infection was correspon ...	2006	16310165
replication and immunoactivity of the recombinant peromyscus maniculatus cytomegalovirus expressing hantavirus g1 glycoprotein in vivo and in vitro.	previously, we have shown that cmv isolated from deer mouse could be used in vivo and in vitro to express sin nombre virus (snv) glycoprotein g1 in deer mice [rizvanov aa, van geelen ag, morzunov s, et al. generation of a recombinant cytomegalovirus for expression of a hantavirus glycoprotein. j.virol. 2003;77(22):12203-10]. in this report, we further characterize replication of wild-type (wt) and recombinant peromyscus cmv (pcmv) in vivo and in vitro using realtime pcr, and infectious center as ...	2006	16125285
a mathematical model for indirectly transmitted diseases.	we consider a mathematical model for the indirect transmission via a contaminated environment of a microparasite between two spatially distributed host populations having non-coincident spatial domains. the parasite is benign in a first population and lethal in the second one. global existence results are given for the resulting reaction-diffusion system coupled with an ordinary differential equation. then, invasion and persistence of the parasite are studied. a simplified model for the transmis ...	2007	16216284
predicting the emergence of human hantavirus disease using a combination of viral dynamics and rodent demographic patterns.	the paper proposes a model explaining the spatial variation in incidence of nephropathia epidemica in europe. we take into account the rodent dynamic features and the replicative dynamics of the virus in animals, high in the acute phase of newly infected animals and low in the subsequent chronic phase. the model revealed that only vole populations with multi-annual fluctuations allow for simultaneously high numbers of infected rodents and high proportions of those rodents in the acute excretion ...	2007	16753079
seaport as a source of hantavirus: a study on isolated isles.	since small mammals from seaports have relative higher seroprevalences of hantavirus, this seroepidemiologic study was conducted on the isles in kinmen and lienchiang counties along the coast of southern mainland china to determine whether seaport may play the role as a source of hantavirus. among six species of small mammals trapped in kinmen county, only male mus musculus (6.7%) and rattus losea (5.3%) were found to be positive. in lienchiang county, five species of small mammals were trapped ...	2007	17365077
clusters of hantavirus infection, southern argentina.	person-to-person transmission of a hantavirus was first confirmed during a 1996 outbreak of hantavirus pulmonary syndrome in southern argentina, where andes virus is endemic. to identify other episodes of secondary transmission, we reviewed reports of 51 cases of hantavirus infection from this region (november 1993-june 2005). nine clusters involving 20 cases (39.2%) were found. two patients, who had symptoms 3 weeks after they shared risks for rodent exposure, were considered a cluster. the oth ...	2007	17370522
isolation and characterization of hantavirus carried by apodemus peninsulae in jilin, china.	to provide a better understanding of hantavirus epidemiology in china, korean field mice (apodemus peninsulae) and striped field mice (apodemus agrarius) were captured in jilin province, china, where haemorrhagic fever with renal syndrome (hfrs) is endemic. hantavirus antigens were detected in eight of the 130 a. peninsulae individuals and in four of the 193 a. agrarius individuals by using an immunofluorescence assay. partial s and m segments were amplified from all of the antigen-positive samp ...	2007	17374775
hantavirus infection in brazil: development and evaluation of an enzyme immunoassay and immunoblotting based on n recombinant protein.	the symptoms of hantavirus pulmonary syndrome may resemble those of other febrile illnesses. the development of an accurate diagnostic test should therefore improve clinical prognosis and be useful in epidemiologic studies. we evaluated the use of a recombinant antigen (rndelta(85)) based on the s-segment sequences of a brazilian hantavirus for detecting immunoglobulin m (igm) and immunoglobulin g (igg) antibodies against hantavirus in an indirect enzyme immunoassay (eia). we assayed 613 serum s ...	2007	17383845
index of suspicion.		2007	17400825
brush mouse (peromyscus boylii) population dynamics and hantavirus infection during a warm, drought period in southern arizona.	we monitored limestone canyon hantavirus (lscv) antibody prevalence, host (brush mouse, peromyscus boylii) abundance, and environmental variables (temperature and rainfall) in brush mice captured on three trapping webs in southern arizona for 5 yr. although seasonal patterns were subtle, we observed large multiyear variation in population abundance and antibody prevalence. limestone canyon hantavirus infection in brush mouse populations varied over time with prevalence ranging from 0% to 33%. at ...	2007	17984263
cluster of cases of hantavirus pulmonary syndrome in alberta, canada.	in may 2005, a cluster of four hantavirus pulmonary syndrome (hps) cases was confirmed in alberta, canada. the cluster is unusual given that three cases were from a single family and involved a 7-year-old child. this is the first family cluster reported in canada and includes one of the youngest cases of hps reported in north america.	2007	17984353
case report of hantavirus pulmonary syndrome and review.	a previously healthy 29-year-old male presented to the emergency department with pleuritic chest pain, nonproductive cough, and dyspnea. he was treated empirically for community acquired pneumonia and discharged to home. two days later, the patient presented with respiratory distress along with neutrophilia, thrombocytopenia, elevated liver function tests, and hemoconcentration. radiographs of his chest showed bilateral lung infiltrates and pleural effusions. he was admitted to the hospital and ...	2007	17694939
ocular features of hantavirus infection.	hantavirus infections are an emerging infectious disease that is beginning to be recognized both worldwide and in india as a cause of hemorrhagic fever that may present as a pulmonary syndrome or as a renal syndrome. reports of ocular involvement are rare and include transient myopia, low intraocular pressure, conjunctival hemorrhages and changes of intraocular dimensions. eleven patients (10 males, one female, mean age 37.6 years) were admitted to the intensive care unit for pyrexia of unknown ...	2007	17699950
[genetic susceptibility to andes hantavirus: association between severity of disease and hla alíeles in chilean patients].	andes hantavirus (andv) infection in chile has a variable clinical expression, and infected individuals may present with different grades of disease severity. this study aimed to determine if clinical expression of andv infection in chilean patients is associated with the hla system. hla alíeles a, b, drb1 and dqb1, were studied in two groups of patients with confirmed andv infection: 41 patients with a mild disease course (without respiratory failure and cardiovascular shock) and 46 patients wi ...	2007	17989838
[hantavirus specific igg antibodies in population of zoologists and forest workers].	serological diagnostic tests were introduced for detection of immunoresponse against to etiological agents of current infectious diseases. interpretation criteria for those tests should eliminate unspecific reactions, cross-reactivity with related viruses as well as traces amounts of immunological response against to the past infections. in the case of past hantaviruses infections for obtaining an adequate seroepidemiological data revalidation of this tests are required. in present studies puuma ...	2007	18069384
[the cross and unspecific reactions in serological examination for antibodies against hantavirus puumala].	a serological survey of 78 zoologist capturing small wild rodents in their environment, 9 patients with suspected hantavirus infections and 21 patients with acute renal dysfunction for antibodies to hantaviruses was conducted in poland. survey was done by the indirect elisa with puumala and hantaan virus antigens. out of the 78 mammalogists 15 were seropositive for hantavirus puumala igg without history of clinical illness. analysis of relation between reactive zoologist's sera igg with antigens ...	2007	18069385
[occurrence of human hantavirus infections in poland].	a spread of hantavirus infections causing hemorrhagic fever with renal syndrome is one of the rising epidemiological problems in the world. there is evidence that this type of viruses exists in almost all european countries. it is known that in poland all the species of wild rodents hosting european hantaviruses are present. however, the data on hantavirus are very limited and information on incidence and prevalence of hantavirus infections among humans and animals in our country is lacking. up ...	2007	18069386
[study on the recombinant expression of hantaan virus protein n and the establishment and application of rnp-igm direct capture elisa].	to clone the gene encoding nucleocapsid protein (np) of hantavirus strain z10 (hv-z10), to construct its prokaryotic expression system as well as to establish a rnp-igm direct capture elisa based on hrp-labeled recombinant np (rnp), in order to detect serum samples of patients suffering from hemorrhagic fever with renal syndrome (hfrs) and to evaluate the effects of detection.	2007	18069562
can modeling of fine-scale spatial patterns of environmental markers of zoonotic infections enhance disease prevention and clinical outcomes?		2007	18165510
a spatial model of shared risk for plague and hantavirus pulmonary syndrome in the southwestern united states.	plague and hantavirus pulmonary syndrome (hps) are severe, often fatal diseases in humans that share a broad epidemiologic focus in the southwestern united states. prevention of these diseases relies heavily on education and reducing rodent abundance in peridomestic environments. resources for these activities are limited. therefore, identifying areas sharing elevated risk for these two relatively rare but severe diseases could be useful for targeting limited public health resources. using logis ...	2007	18165511
mode of infection of hokkaido virus (genus hantavirus) among grey red-backed voles, myodes rufocanus, in hokkaido, japan.	hokkaido virus (hokv) is a member of the genus hantavirus, in the family bunyaviridae. to investigate hokv infection in the host myodes rufocanus, the grey red-backed vole, 199 animals were captured at tobetsu (october 2004 and july 2005) and nakagawa (october 2004) in hokkaido, japan, for detection of antibody, antigen, and viral rna. in the surveys in tobetsu (2004) and nakagawa (2004), seropositive animals were detected at a frequency of 6.0% (5/84) and 10.4% (5/48), respectively. no seroposi ...	2007	18037785
[hantaviruses. infection numbers increase!].		2007	18062336
environmental conditions and puumala virus transmission in belgium.	non-vector-borne zoonoses such as puumala hantavirus (puuv) can be transmitted directly, by physical contact between infected and susceptible hosts, or indirectly, with the environment as an intermediate. the objective of this study is to better understand the causal link between environmental features and puuv prevalence in bank vole population in belgium, and hence with transmission risk to humans. our hypothesis was that environmental conditions controlling the direct and indirect transmissio ...	2007	18078526
[identification of a functional itam-like sequence within g1 cytoplasmic tail of hantaan virus].	the g1 cytoplasmic tail of hantaan virus (htnv) harbors a highly conserved region, which is homologous to immunoreceptor tyrosine-based activation motifs (itam) and is termed the itam-like sequence. to demonstrate the potential signal-transducing activity of g1 itam-like sequence resembling the canonical itam within immune and endothelial cells, a series of experiments were performed to define its interaction with cellular kinases. the synthesized g1 itam-like peptide was shown to coprecipitate ...	2007	18092677
thottapalayam virus, a prototype shrewborne hantavirus.	thottapalayam virus (tpmv) has been placed in the genus hantavirus of the family bunyaviridae by virtue of its morphologic features and overall genetic similarities to well-characterized rodentborne hantaviruses. this virus has been isolated from the asian house shrew (suncus murinus); however, whether tpmv is naturally harbored by an insectivore host or represents spillover from a rodent reservoir host is unknown. our analysis of published and unpublished data on the experimental host range, ge ...	2007	18214168