Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| genetic and antigenic characterization of bovine viral diarrhoea virus type 2 isolated from cattle in india. | previous studies have shown that bovine viral diarrhoea virus type 1 (bvdv-1) subtype b is predominantly circulating in indian cattle. during testing for exotic pestiviruses between 2007 and 2010, bvdv-2 was identified by real time rt-pcr in two of 1446 cattle blood samples originating from thirteen states of india. the genetic analysis of the isolated virus in 5' utr, n(pro), entire structural genes (c, e(rns), e1 and e2), nonstructural genes ns2-3 besides 3' utr demonstrated that the nucleotid ... | 2010 | 21112633 |
| swine adipose stromal cells loaded with recombinant bovine herpesvirus 4 virions expressing a foreign antigen induce potent humoral immune responses in pigs. | increasingly effective vaccination strategies are needed to counteract the high incidence of contagious diseases associated with intensive swine breeding. recombinant viral vaccines are a promising new avenue in this direction. key features of viral vectors suitable for immunoprophylaxis are safety, ease of manipulation and the ability to replicate in a variety of hosts. most of the above requirements are met by bovine herpesvirus 4 (bohv-4), a non-pathogenic dsdna virus capable of infecting a b ... | 2010 | 21115049 |
| clinical bovine viral diarrhoea virus infection in jordan. | a 1-year-old holstein friesian heifer was presented for anorexia and acute diarrhoea. the heifer was born and raised at the farm. bovine viral diarrhoea virus (bvdv) infection was diagnosed using clinical signs and rt-pcr. clinical bvdv infection has never been reported in jordan. | 2010 | 21117287 |
| seroprevalence of infectious bovine rhinotracheitis and bovine viral diarrhea virus type 1 and type 2 in non-vaccinated cattle herds in the pacific region of central costa rica. | the objectives of this cross-sectional study were to estimate the seroprevalence of infectious bovine rhinotracheitis (ibr, bhv-1) and bovine viral diarrhea virus (bvdv) in a population of non-vaccinated, double purpose, dairy and beef herds in the pacific region of central costa rica. blood samples were collected from a total of 496 animals from 35 herds. sera were tested for antibodies against bhv-1(ibr) and bvdv types 1 and 2 using serum neutralization test. the average number of animals test ... | 2010 | 21128116 |
| [new guidelines for investigating and evaluating the virucidal activity of disinfectants]. | the diversity of viruses that cause human infectious diseases and problems in culturing some of them, such as hepatitis a, b, and c viruses and hiv, necessitate a search for test viruses that are closely resistant to disinfectants. the early methodical documents and the new one "guidelines for investigating and evaluating the virucidal activity of disinfectants" (my 3.5.2431-08) include poliovirus (vaccine strain) as one of the test viruses. adenovirus and thermoresistant parvovirus are addition ... | 2010 | 21344697 |
| characterization and purification of recombinant bovine viral diarrhea virus particles with epitope-tagged envelope proteins. | bovine viral diarrhea virus (bvdv) belongs to the genus pestivirus within the family flaviviridae. the lipid membrane of the virions is supposed to contain the three glycosylated envelope proteins e(rns), e1 and e2, but detailed studies of virus assembly are complicated because no efficient purification method for pestiviruses has been described so far. in this study, we generated infectious bvdv with n-terminally flag-tagged e(rns) or e2 proteins, respectively. the expression of the epitope-tag ... | 2011 | 21346033 |
| isolation and identification of a bovine viral diarrhea virus from sika deer in china. | bovine viral diarrhea virus (bvdv) infections continue to cause significantly losses in the deer population. better isolation and identification of bvdv from sika deer may contribute significantly to the development of prophylactic therapeutic, and diagnostic reagents as well as help in prevention and control of bvdv. however, isolation and identification of bvdv from sika deer is seldom reported in literature. in this study, we collected some samples according to clinical sign of bvdv to isolat ... | 2011 | 21352530 |
| fetal protection in heifers vaccinated with a modified-live virus vaccine containing bovine viral diarrhea virus subtypes 1a and 2a and exposed during gestation to cattle persistently infected with bovine viral diarrhea virus subtype 1b. | to determine efficacy of a modified-live virus (mlv) vaccine containing bovine viral diarrhea virus (bvdv) 1a and 2a against fetal infection in heifers exposed to cattle persistently infected (pi) with bvdv subtype 1 b. | 2011 | 21355740 |
| comparison of viral replication and ifn response in alpaca and bovine cells following bovine viral diarrhea virus infection. | alpacas develop diminished disease following bovine viral diarrhea virus (bvdv) infection compared to cattle. we hypothesized that alpaca and bovine cells have differential permissiveness and responses to bvdv infection. to characterize alpaca testicular (at) and bovine turbinate (bt) cells bvdv infection permissiveness, viral replication and interferon (ifn) synthesis was evaluated. bvdv replicated 3-4 logs lower in at cells with diminished antigen deposition compared to bt cells. bvdv infectio ... | 2011 | 21356540 |
| rna recombination in pestiviruses: cellular rna sequences in viral genomes highlight the role of host factors for viral persistence and lethal disease. | persistence of the positive strand rna virus bovine viral diarrhea virus (bvdv) in its host may last for years. however, it frequently ends in lethal disease triggered by emerging virus mutants created by rna recombination. those mutant genomes often encompass cellular mrna fragments. persistence of bvdv depends on a mechanism limiting viral rna replication efficiency. this restriction is based on the dependency of a viral protease on a cellular cofactor available only in limiting amounts. virus ... | 2011 | 21358277 |
| bovine viral diarrhea (bvd) eradication in switzerland--experiences of the first two years. | a national eradication programme was designed with the aim of achieving total freedom from bovine viral diarrhea virus (bvdv) infection in the swiss cattle population. the eradication programme consisted of testing every swiss bovine for antigen, culling virus-positive animals and applying movement restrictions. starting in 2008, the campaign achieved the goal of reducing the proportion of newborn calves that were virus-positive from 1.8% to under 0.2% within two years (situation in september 20 ... | 2011 | 21371766 |
| change in predominance of bovine viral diarrhea virus subgenotypes among samples submitted to a diagnostic laboratory over a 20-year time span. | although the causative agent of bovine viral diarrhea was initially categorized as 1 species, phylogenetic analysis revealed that these viruses belong to 2 different species, bovine viral diarrhea virus 1 (bvdv-1) and bvdv-2, with 2-11 subgenotypes within each species. distribution of species and subgenotypes has been shown to vary with geographic region. whether distribution shifts over time is not known. surveys conducted between 1994 and 2008 reported 3 subgenotypes circulating among cattle i ... | 2011 | 21398436 |
| a simulation model to quantify the value of implementing whole-herd bovine viral diarrhea virus testing strategies in beef cow-calf herds. | although numerous diagnostic tests are available to identify cattle persistently infected (pi) with bovine viral diarrhea virus (bvdv) in cow-calf herds, data are sparse when evaluating the economic viability of individual tests or diagnostic strategies. multiple factors influence bvdv testing in determining if testing should be performed and which strategy to use. a stochastic model was constructed to estimate the value of implementing various whole-herd bvdv cow-calf testing protocols. three c ... | 2011 | 21398437 |
| three clusters of bovine kobuvirus isolated in korea, 2008-2010. | fecal samples (n = 107) were collected from cattle with ascertained or suspected diarrheal disease on korean farms during 2008-2010. of these, 37 samples tested positive for bovine kobuvirus. the 37 positive samples came from 32 cattle that exhibited diarrhea and five cattle that were non-diarrhetic. the majority of the virus-positive feces samples were from calves under 1 month of age (n = 25). nine of the 37 cattle infected with bovine kobuvirus were confirmed to have a co-infection with other ... | 2011 | 21399921 |
| inhibition of bovine viral diarrhea virus rna synthesis by thiosemicarbazone derived from 5,6-dimethoxy-1-indanone. | in the present work, we described the activity of the thiosemicarbazone derived from 5,6-dimethoxy-1-indanone (tsc), which we previously characterized as a new compound that inhibits bovine viral diarrhea virus (bvdv) infection. we showed that tsc acts at a point of time that coincides with the onset of viral rna synthesis and that it inhibits the activity of bvdv replication complexes (rcs). moreover, we have selected five bvdv mutants that turned out to be highly resistant to tsc but still sus ... | 2011 | 21430053 |
| experimental infection of colostrum-deprived calves with bovine viral diarrhea virus type 1a isolated from free-ranging white-tailed deer (odocoileus virginianus). | the objective of this study was to experimentally infect calves with bovine viral diarrhea virus (bvdv) isolated from free-ranging white-tailed deer. twelve colostrum-deprived male holstein calves were used. eight were inoculated intranasally with a bvdv type 1a isolated from free-ranging white-tailed deer, and the other four were inoculated with the cell culture medium only and served as a control group. whole blood, saliva, and nasal and rectal secretions were collected on days 0, 3, 7, 10, 14 ... | 2011 | 21461198 |
| a serological investigation of pestiviruses in sheep in eastern border of turkey. | all pestiviruses are important veterinary pathogens causing economic losses in cattle, sheep, and pigs. in this study, blood samples randomly collected from 465 sheep were analysed for the presence of antibodies to pestiviruses (bovine viral diarrhea virus, border disease virus) using an enzyme-linked immunosorbent assay in the province of van and their towns. the seroprevalance were estimated as 75.9% and 60.0-82.5% in the sampled animals and sampled towns, respectively. the results revealed th ... | 2011 | 21461731 |
| genetic change in the open reading frame of bovine viral diarrhea virus is introduced more rapidly during the establishment of a single persistent infection than from multiple acute infections. | bovine viral diarrhea viruses (bvdv) are ubiquitous viral pathogens of cattle with a high degree of sequence diversity amongst strains circulating in livestock herds. the driving force behind change in sequence is not well established but the inaccurate replication of the genomic rna by a viral rna polymerase without proof-reading capabilities as well as immune pressure on immunodominant proteins are thought to play major roles. additionally, it is not clear when the majority of changes are intr ... | 2011 | 21470568 |
| hepatic immune response in calves during acute subclinical infection with bovine viral diarrhoea virus type 1. | eight colostrum-deprived calves aged 8-12weeks were inoculated intranasally with a non-cytopathic strain of bovine viral diarrhoea virus (bvdv) genotype-1 and the effects on the hepatic immune response were studied. two calves were sacrificed at each of 3, 6, 9 and 14days post-inoculation (dpi) and two uninoculated animals were used as negative controls. bvdv was detected in hepatic macrophages and monocytes from 3 to 14dpi and in küpffer cells (kcs) from 6 to 14dpi. increases in the numbers of ... | 2011 | 21470882 |
| antibody responses against non-structural protein 3 of bovine viral diarrhoea virus in milk and serum samples from animals immunised with an inactivated vaccine. | antibodies against non-structural protein 3 (ns3, p80) of bovine viral diarrhoea virus (bvdv) were determined in milk from cows vaccinated with an inactivated bvdv vaccine and compared to serum antibody levels. animals in one herd were vaccinated with an inactivated bvdv vaccine according to the standard protocol and animals from a second herd with an intensive schedule. serum and milk samples were tested for bvdv ns3 antibodies using five commercial elisas. with a few exceptions, vaccination ac ... | 2011 | 21482158 |
| long-term clinicopathological characteristics of alpacas naturally infected with bovine viral diarrhea virus type ib. | background: substantial bovine viral diarrhea virus (bvdv)-related production losses in north american alpaca herds have been associated with bvdv type ib infection. objectives: to classify and differentiate the long-term clinicopathological characteristics of bvdv type ib infection of alpaca crias, after natural virus exposure. we hypothesized that persistently infected (pi) alpacas specifically demonstrate growth retardation, clinicopathological evidence of opportunistic infections, and early ... | 2011 | 21488962 |
| efficacy of the international embryo transfer society (iets) washing procedure for rendering oocytes matured in vitro free of bovine viral diarrhea virus (bvdv). | to ensure the freedom of embryos from pathogenic agents prior to embryo transfer (et), a specific sanitary washing procedure has been recommended by the international embryo transfer society (iets). in the present study, the efficacy of removing the bovine viral diarrhea virus (bvdv) from cumulus-free matured oocytes at the stage of extruded first polar body (n = 240) was evaluated, using the iets-recommended 10 sequential wash procedure, after exposure in vitro to bvdv type 2 (strain pa-131, 1 ... | 2011 | 21497387 |
| species characterization in the genus pestivirus according to palindromic nucleotide substitutions in the 5'-untranslated region. | the palindromic nucleotide substitutions (pns) at the three variable loci (v1, v2 and v3) in the 5'-untranslated region (utr) of the pestivirus genome have been considered for taxonomical segregation of the species, through the evaluation of 534 strains. on the basis of qualitative and quantitative secondary structure characteristics, species have been identified within the genus, determining genetic distances between species isolates, clarifying borderline and multirelated sequences, and charac ... | 2011 | 21514324 |
| development of a triplex taqman real-time rt-pcr assay for differential detection of wild-type and hclv vaccine strains of classical swine fever virus and bovine viral diarrhea virus 1. | in this study, genomic sequences of pestiviruses available in genbank were aligned to design three primer pairs and taqman probes: two targeting the ns5a region of the viral genome of classical swine fever virus (csfv) for the differentiation of wild-type csfv and hog cholera lapinized vaccine (hclv) vaccine, and one targeting the 5'-untranslated region of bovine viral diarrhea virus 1 (bvdv-1). with these primers and probes, a triplex taqman real-time rt-pcr assay was developed for differentiat ... | 2011 | 21536312 |
| protection from persistent infection with a bovine viral diarrhea virus (bvdv) type 1b strain by a modified-live vaccine containing bvdv types 1a and 2, infectious bovine rhinotracheitis virus, parainfluenza 3 virus and bovine respiratory syncytial virus. | recent studies showed that bvdv-1b subgenotype is dominant in north and south american field bvdv isolates. however, nearly all commercially available bvdv-1 vaccines contain bvdv-1a strains. in order to study the efficacy of bvdv-1a vaccine against bvdv-1b infection, this study was designed to evaluate a modified-live vaccine (mlv) containing bvdv-1a and bvdv-2 strains for its efficacy in prevention of persistent infection of fetuses against bvdv-1b strain, when the heifers were vaccinated prio ... | 2011 | 21596076 |
| investigation of a dual fetal infection model with bovine viral diarrhoea viruses (bvdv)-1 and bvdv-2. | two studies were performed in pregnant heifers to determine whether inoculation with two bovine viral diarrhoea viruses (bvdv), one bvdv-1 and one bvdv-2, inoculated separately into either nostril, results in fetal infection with both viruses. dual transplacental infection of the fetus with bvdv-1 and bvdv-2 was observed in one case, but not consistently. | 2011 | 21597952 |
| characterization of apoptosis pathways (intrinsic and extrinsic) in lymphoid tissues of calves inoculated with non-cytopathic bovine viral diarrhoea virus genotype-1. | previous studies have shown that activation of effector caspase-3 is associated with the apoptosis of lymphocytes occurring during infection with bovine viral diarrhoea virus (bvdv); however, the regulation of the apoptosis pathways that induce cell death via activation of effector caspase-3 has not yet been clarified. the aim of this study was to examine immunohistochemically the expression of cleaved caspase (ccasp)-8 (initiator caspase of the extrinsic pathway), ccasp9 (initiator caspase of t ... | 2011 | 21612789 |
| acridine derivatives as anti-bvdv agents: a preliminary study. | twenty-six 9-aminoacridine derivatives were evaluated in cell-based assays for cytotoxicity and antiviral activity against a panel of 10 rna and dna viruses. while seven compounds (9, 10, 14, 19, 21, 22, 24) did not affect any virus and two (6, 11) were moderately active against cvb-5 or reo-1, 17 compounds exhibited a marked specific activity against bvdv, prototype of pestiviruses which are responsible for severe diseases of livestock. most anti-bvdv agents showed ec(50) values in the range 0. ... | 2011 | 21619897 |
| single dilution avidity-blocking elisa as an alternative to the bovine viral diarrhea virus neutralization test. | this study describes the development and validation of a blocking elisa that measures avidity of bvdv-specific immunoglobulins (igs) as an alternative to the classic virus neutralization test. the assay comprises a recombinant soluble e2 glycoprotein as target antigen, a neutralizing serum as detector antibody and a washing-step with a chaotropic agent to determine bvdv-specific igs avidity. avidity-blocking elisa was validated with 100 negative and 87 positive bvdv-neutralization serum samples ... | 2011 | 21621555 |
| kinetics of uv(254) inactivation of selected viral pathogens in a static system. | aims: the objective of this study was to estimate uv(254) inactivation constants for four viral pathogens: influenza virus type a, porcine respiratory and reproductive syndrome virus (prrsv), bovine viral diarrhoea virus (bvdv) and reovirus. methods and results: viruses in culture medium were exposed to one of nine doses of uv(254) and then titrated for infectious virus. analysis showed that viral inactivation by uv(254) was more accurately described by a two-stage inactivation model vs a stan ... | 2011 | 21624016 |
| the effects of exposure of susceptible alpacas to alpacas persistently infected with bovine viral diarrhea virus. | reports of bovine viral diarrhea virus (bvdv) infections in alpacas have been increasing in recent years but much is still unknown about the mechanisms of disease in this species. this report characterizes the transmission of bvdv from persistently infected (pi) alpacas to bvdv naïve alpacas, documents shedding patterns, and characterizes the disease effects in both pi and transiently infected alpacas. two pi alpacas shed bvdv type 1b virus in most body fluids, and commonly available diagnostic ... | 2011 | 21629418 |
| isolation and genetic analysis of bovine viral diarrhea virus from infected cattle in indiana. | species and biotype distribution was determined in 44 bovine viral diarrhea virus- (bvdv-) positive samples submitted to the animal disease diagnostic laboratory (addl) in indiana during 2006-2008. bvdv rna was detected in the 5'-untranslated region and n(pro) region using reverse transcriptase pcr followed by sequencing analysis of the pcr product. additionally, cases were classified into one of six categories according to history and/or lesions: acute symptomatic, hemorrhagic, respiratory dist ... | 2011 | 21647344 |
| seroepidemiological study of bovine respiratory viruses (brsv, bohv-1, pi-3v, bvdv, and bav-3) in dairy cattle in central region of iran (esfahan province). | respiratory diseases in calves are responsible for major economic losses in both beef and dairy production. several viruses, such as bovine respiratory syncytial virus (brsv), bovine herpes virus-1 (bohv-1), bovine parainfluenza virus-3 (bpi-3v), bovine viral diarrhea virus (bvdv), and bovine adenoviruses (bav), are detected in most clinical cases with respiratory signs. the aim of this study is to define seroprevalences of five major viral causes of bovine respiratory infections in cattle in ce ... | 2011 | 21667075 |
| inactivation of enveloped and non-enveloped viruses on seeded human tissues by gamma irradiation. | human tissue allografts are widely used in a variety of clinical applications with over 1.5 million implants annually in the us alone. since the 1990s, most clinically available allografts have been disinfected to minimize risk of disease transmission. additional safety assurance can be provided by terminal sterilization using low dose gamma irradiation. the impact of such irradiation processing at low temperatures on viruses was the subject of this study. in particular, both human tendon and co ... | 2011 | 21809182 |
| development of one-step sybr green real-time rt-pcr for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional rt-pcr. | abstract: background: bovine viral diarrhea virus (bvdv) is a worldwide pathogen in cattle and acts as a surrogate model for hepatitis c virus (hcv). one-step real-time fluorogenic quantitative reverse transcription polymerase chain reaction (rt-pcr) assay based on sybr green i dye has not been established for bvdv detection. this study aims to develop a quantitative one-step rt-pcr assay to detect bvdv type-1 in cell culture. results: one-step quantitative sybr green i rt-pcr was developed by a ... | 2011 | 21798067 |
| endotoxin-free purification for the isolation of bovine viral diarrhoea virus e2 protein from insoluble inclusion body aggregates. | abstract: background: protein expression in escherichia coli may result in the recombinant protein being expressed as insoluble inclusion bodies. in addition, proteins purified from e. coli contain endotoxins which need to be removed for in vivo applications. the structural protein, e2, from bovine viral diarrhoea virus (bvdv) is a major immunogenic determinant, and is an ideal candidate as a subunit vaccine. the e2 protein contains 17 cysteine residues creating difficulties in e. coli expressio ... | 2011 | 21787435 |
| epidemiological survey of border disease virus among sheep from northern districts of japan. | the first epidemiological survey of border disease virus (bdv) was undertaken in small ruminants in japan. ovine sera, collected from the northern prefectures of hokkaido, aomori and iwate, were examined for the presence of antibodies against bdv using the neutralization peroxidase-linked antibody test. twenty-nine (17.6%) of one hundred and sixty-five samples were seropositive for bdv. results were specific, excluding cross-reactions with bovine viral diarrhea virus (bvdv). only one sample (0.6 ... | 2011 | 21778667 |
| virus distribution and role of thymic macrophages during experimental infection with noncytopathogenic bovine viral diarrhea virus type 1. | thymic depletion, presence of viral antigen, and changes in distribution and cytokine production of thymic macrophages were investigated in calves experimentally infected with a noncytopathogenic bovine viral diarrhea virus type (bvdv) 1 strain. ten clinically healthy colostrum-deprived calves were used. eight calves were inoculated with the virus and two were used as uninfected controls. calves were sedated and euthanized in batches between 3 and 14 days postinoculation. at necropsy, thymus sam ... | 2011 | 21768605 |
| seroepidemiology of bovine viral diarrhoea virus (bvdv) in the adamawa region of cameroon and use of the spot test to identify herds with pi calves. | bovine viral diarrhoea, caused by the bovine viral diarrhoea virus (bvdv) in the pestivirus genus of the flaviviridae, is one of the most important diseases of cattle world wide causing poor reproductive performance in adult cattle and mucosal disease in calves. in addition it causes immunosuppression and increased susceptibility to other infections, the impact of which is uncertain, particularly in sub-saharan africa where animals are exposed to a much wider range and higher intensity of infect ... | 2011 | 21754993 |
| comparative structural studies of bovine viral diarrhea virus ires rna. | the internal ribosomal entry site (ires) rna of bovine viral diarrhea virus (bvdv) has been implicated in virus propagation. to gain insight into the structure and potential function of the bvdv ires rna, we collected and aligned 663 of its sequences. compensatory watson-crick and wobble g-àu pairs were investigated to establish phylogenetically supported secondary structures for each of the bvdv ires rna sequences. the extensively folded bvdv ires rnas were composed of helices 2, 3 and 4. helix ... | 2011 | 21683744 |
| prevalence of respiratory pathogens in diseased, non-vaccinated, routinely medicated veal calves. | the prevalence of respiratory pathogens in diseased veal calves was determined in 24 respiratory disease outbreaks in 15 herds in belgium. bacteria were cultured from nasopharyngeal swabs and seroconversion against viruses and mycoplasma bovis was determined on paired sera. at the individual calf level, mycoplasma species, mannheimia haemolytica and pasteurella multocida, were isolated from 70.5 per cent, 21.5 per cent and 26.0 per cent of swabs, respectively. at the herd level, the presence of ... | 2011 | 21831999 |
| seroprevalence and risk factors associated with bovine viral diarrhea virus (bvdv) infection in non-vaccinated dairy and dual purpose cattle herds in ecuador. | a cross-sectional study was carried out to determine the seroprevalence and risk factors associated with bovine viral diarrhea virus (bvdv) infection in non-vaccinated dairy and dual-purpose cattle herds from ecuador. a total of 2,367 serum samples from 346 herds were collected from june 2008 through february 2009. a questionnaire, which included variables related to cattle, health, management measures, and the environment, was filled out in each herd. a commercial indirect enzyme-linked immunos ... | 2011 | 21822791 |
| evaluation of the effects of long-term storage of bovine ear notch samples on the ability of 2 diagnostic assays to identify calves persistently infected with bovine viral diarrhoea virus. | research aimed at optimising diagnostic laboratory procedures is central to the development of effective bovine viral diarrhoea virus (bvdv) control programmes. bvdv is a single-stranded rna virus that crosses the placenta to infect foetuses, resulting in reproductive losses due to foetal death or persistently infected calves that die early in life. persistently infected animals are widely accepted to be the primary reservoir of bvdv and the largest source of infection. this poses important chal ... | 2011 | 21826833 |
| fetal protection against bovine viral diarrhoea type 1 virus infection after one administration of a live-attenuated vaccine. | a modified-live vaccine has been shown previously to prevent fetal infection with bovine viral diarrhoea virus (bvdv)-2 and, to some extent bvdv-1, when used in association with an inactivated vaccine in a two-step vaccination protocol. in this challenge study, the modified-live vaccine used alone was able to protect 13 heifers between 49 and 96days of gestation at challenge from leucopenia and virus replication and, for a 4-month period, to prevent fetal infection. the efficacy of the bvdv-1f 2 ... | 2011 | 21689959 |
| serological evidence for bvdv-1 infection in goats in poland - short communication. | a serological survey was conducted in 2007 in the breeding goat population in poland to gain insights into the epidemiology of pestivirus infection. all breeding herds were included in the study and representative serum samples were taken in each herd to evaluate herd-level seroprevalence at 10% expected individual-level prevalence and 95% level of confidence. altogether 1060 serum samples from 49 herds were tested with blocking elisa and then the positive and inconclusive results were confirmed ... | 2011 | 21727071 |
| molecular detection of pestiviruses in aborted foetuses from provinces in northern turkey. | all pestiviruses are important veterinary pathogens causing economic losses in cattle, sheep and pigs. besides the important economical losses, pestiviruses may compromise the normal immune response to other pathogens and increase the severity of other infections in sheep. in this study, aborted foetuses (cattle and sheep) in either coastal or inland black sea region of turkey were surveyed for the presence of rna from pestiviruses (bovine viral diarrhoea virus (bvdv), border disease virus (bdv) ... | 2011 | 21842445 |
| epizootiologic investigations of selected abortive agents in free-ranging alpine ibex (capra ibex ibex) in switzerland. | in the early 2000s, several colonies of alpine ibex (capra ibex ibex) in switzerland ceased growing or began to decrease. reproductive problems due to infections with abortive agents might have negatively affected recruitment. we assessed the presence of selected agents of abortion in alpine ibex by serologic, molecular, and culture techniques and evaluated whether infection with these agents might have affected population densities. blood and fecal samples were collected from 651 ibex in 14 col ... | 2011 | 21719818 |
| in vitro permissivity of bovine peripheral blood mononuclear cells to bovine viral diarrhoea virus is dependent on the animal specific immune status. | the in vitro permissivity to infection with homologous and heterologous bovine viral diarrhoea virus (bvdv) strains of bovine peripheral blood mononuclear cells (pbmcs) from eight na+»ve and eight bvdv-1b immune animals was studied. four reference strains (bvdv-1a nadl, bvdv-1b ny-1, bvdv-2 125 and bvdv-2 890) were selected, based on genotype, prevalence and biotype. virus neutralizing antibody titres were determined at bleeding and the viral loads were measured in pbmcs by end point titration i ... | 2011 | 21689960 |
| establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species. | a sheep kidney-derived cell line, flk-n3, was successfully established after serial (>100) passages. persistent infection of this cell line with viruses and mycoplasma was not detected. the cells grew well and showed susceptibility to a wide variety of viruses derived from ovine, bovine, and porcine species, including orf virus, maedi visna virus, bovine herpesvirus 1, bovine parainfluenza virus 3, bovine viral diarrhea viruses 1 and 2, bovine coronavirus, bovine respiratory syncytial virus, bov ... | 2011 | 21695582 |
| severe disease in neonatal calves with detection of cytopathic bvdv. | 2011 | 21709044 | |
| genetic and splice variations of bos taurus cd46 shift cell permissivity to bvdv, the bovine pestivirus. | the pestivirus bovine viral diarrhea virus (bvdv) is known to bind to the cd46 molecule, which subsequently promotes entry of the virus. mapping of the bvd-virion-binding site has shown that two peptides, 66eqiv69 and 82gqvlal87, located on antiparallel beta sheets in the most distal complement control protein module (ccp1), provide the attachment platform. in the present study, we reveal the existence of ten distinct allelic versions of the ccp1 module, varying significantly in frequency among ... | 2011 | 21680116 |
| Methods to select suitable fetal bovine serum for use in quality control assays for the detection of adventitious viruses from biological products. | Production of biological products, especially vaccines, usually requires materials derived from animals, and there are always risks that animal pathogens derived from these materials could contaminate the final products. Detection of adventitious agents is performed by quality control tests. In these biological assays, animal derived materials are also used and another problem arises, as fetal bovine serum (FBS) is used as an ingredient in tissue culture media. FBS contaminated with bovine viral ... | 2011 | 21719306 |
| Postweaning multisystemic wasting syndrome produced in gnotobiotic pigs following exposure to various amounts of porcine circovirus type 2a or type 2b. | In late 2005, a postweaning, high mortality syndrome spread rapidly through finishing barns in swine dense areas of the United States. Diagnostic investigations consistently detected porcine circovirus type 2 (PCV2) from diseased tissues. Subsequent genetic analysis revealed that the infectious agent was a PCV2 type termed "PCV2b". Prior to late 2004, only the PCV2a type, but not PCV2b, had been reported in North America. In this communication, we produce severe postweaning multisystemic wasting ... | 2011 | 21684696 |
| experimental infection of cattle, sheep and pigs with 'hobi'-like pestivirus. | to date, limited information is available on the ability of 'hobi'-like pestiviruses (putative bovine viral diarrhoea 3) to infect and cause disease in animal species traditionally affected by pestiviruses. in order to obtain new insights into host range and pathogenic potential of this atypical pestivirus, bvdv-seronegative calves (n=5), lambs (n=5) and piglets (n=5) were experimentally infected with the european 'hobi'-like strain italy-1/10-1, whereas two animals per species served as uninfec ... | 2011 | 21955447 |
| innocuousness and safety of classical swine fever marker vaccine candidate cp7_e2alf in non-target and target species. | chimeric pestivirus cp7_e2alf is a promising live marker vaccine candidate against classical swine fever. prior to a possible application in the field, several safety aspects have to be addressed. due to the fact that cp7_e2alf is based on a bovine viral diarrhea virus backbone, its behavior in ruminants is of particular interest. in the framework of this study, its innocuousness in non-target species was addressed by inoculation of calves, young goats, lambs, and rabbits. to this means, high ti ... | 2011 | 22064266 |
| yeast-surface expressed bvdv e2 protein induces a th1/th2 response in naïve t cells. | yeast species such as saccharomyces cerevisiae are known to be potent activators of the immune system. s. cerevisiae activates the innate immune system by engaging pattern recognition receptors such as toll like receptor 2 (tlr2) and dectin-1. in the current project, we express the immunogenic envelope protein e2 of bovine viral diarrhoea virus (bvdv) on the surface of the yeast s. cerevisiae. after successful expression, components of the innate and adaptive immune response induced by the recom ... | 2011 | 22067741 |
| Serological evaluation of relationship between viral pathogens (BHV-1, BVDV, BRSV, PI-3V, and Adeno3) and dairy calf pneumonia by indirect ELISA. | In this study, viral pathogens associated with nine outbreaks of naturally occurring dairy calf pneumonia in Mashhad area of Khorasan Razavi province from September 2008 to May 2009 were assessed. Five diseased calves from each farm were chosen for examination. Acute and convalescent serum samples were taken from calves with signs of respiratory disease. Sera were analyzed for antibodies to bovine viral diarrhea virus (BVDV), bovine herpesvirus type 1 (BHV-1), bovine respiratory syncytial virus ... | 2011 | 22198538 |
| genetic typing and epidemiologic observation of bovine viral diarrhea virus in western china. | whole blood samples (472) were collected during the period of 2006-2008 from 15 cattle farms in seven districts of the xinjiang uygur autonomous region, which is a major pastoral area and represents 1/6 of chinese territory. bovine viral diarrhea virus (bvdv) was detected by nested reverse transcription polymerase chain reaction assay (rt-npcr) and sequenced within the 5'-untranslated region of the genome. approximately 43% (202 samples) were bvdv-positive. of the positive samples, 24 were chose ... | 2011 | 21132458 |
| genetic and antigenic typing of border disease virus (bdv) isolates from italy reveals the existence of a novel bdv group. | border disease virus belongs to the pestivirus genus, within the family flaviviridae. genetic analysis of pestiviruses isolated from sheep in continental europe have led to the proposal that bdv isolates are segregated into at least seven clusters. in 2005 the molecular analysis of an italian caprine bdv strain provided evidence for the presence of an atypical pestivirus, which may represent the first member of a putative novel pestivirus sub-group. to further build on this study, ovine pestivir ... | 2011 | 20656426 |
| experimental infection of early pregnant cows with bovine viral diarrhea virus: transmission of virus to the reproductive tract and conceptus. | the objective of this study was to chronologically investigate the effect of bovine viral diarrhea virus (bvdv) infection on early pregnant cows before placenta formation. six cows were intravenously inoculated with either bvdv (treated, n=4) or growth medium (control, n=2) (day 0) at day 26 of pregnancy. two treated cows and one control cow were euthanized on day 3 post-infection and the remaining animals were euthanized on day 6. bvdv was isolated from maternal tissues such as lymphoid or repr ... | 2011 | 20553701 |
| antibody responses to inactivated vaccines and natural infection in cattle using bovine viral diarrhoea virus elisa kits: assessment of potential to differentiate infected and vaccinated animals. | bovine viral diarrhoea virus (bvdv) is one of the most common and economically important viral infections of cattle. as vaccination is common in most european countries, differentiation between infected and vaccinated animals is one of the key challenges facing bvdv eradication campaigns. this study was designed to compare the ability of commercial elisa kits to differentiate antibodies generated following vaccination with four different commercial inactivated bvdv vaccines from antibodies gener ... | 2011 | 20074985 |
| reactivity and prevalence of neutralizing antibodies against japanese strains of bovine viral diarrhea virus subgenotypes. | bovine viral diarrhea virus (bvdv) field isolates show genetic and antigenic diversity. at least 14 subgenotypes of bvdv-1 and 4 of bvdv-2 have been identified in artiodactyla worldwide. of these, 6 subgenotypes of bvdv-1 and 1 of bvdv-2 have been isolated in japan. previously, we reported that each subgenotype virus expresses different antigenic characteristics. here we investigated the reactivity of neutralizing antibodies against representative strains of japanese bvdv subgenotypes using sera ... | 2011 | 19931181 |
| a serologic survey of viral infections in captive ungulates in turkish zoos. | zoos and zoologic gardens make optimal environments for interspecies transmission of viral infections. there are seven zoos and several small zoologic collections in turkey. this study aimed to determine the current status of viral infections in captive ungulates living in these environments. blood samples were taken from 163 captive animals from two zoos. there were 39 cameroon sheep (ovis ammon f aries), 11 barbary sheep (ammotragus lervia), 57 pygmy goats (capra hircus), 9 angora goats (capra ... | 2011 | 22946369 |
| bovine viral diarrhea virus strain- and cell type-specific inhibition of type i interferon pathways. | 2011 | 21270161 | |
| detection of colostrum-derived alloantibodies in calves with bovine neonatal pancytopenia. | bovine neonatal pancytopenia (bnp) is an emerging calf disease of unknown cause characterized by a pronounced susceptibility to bleeding as a result of a pancytopenia and bone marrow depletion. in this study we investigated whether this phenomenon is related to colostrum-derived alloantibodies directed against neonatal leukocytes. in a first experiment and using a flow cytometric approach sera from 6 bnp-dams (had given birth to bnp-calves; vaccinated against bovine viral diarrhea virus [bvdv]) ... | 2011 | 21272941 |
| development and evaluation of a luminex multiplex serology assay to detect antibodies to bovine herpes virus 1, parainfluenza 3 virus, bovine viral diarrhoea virus, and bovine respiratory syncytial virus, with comparison to existing elisa detection methods. | detection of circulating antibodies to bovine herpes virus 1 (bhv-1), parainfluenza 3 virus (pi3v), bovine viral diarrhoea virus (bvdv) and bovine respiratory syncytial virus (brsv) using elisa is widely used for veterinary diagnostics and surveillance. in this paper, the potential of a multiplex serology test based on luminex technology, where all antibodies are simultaneously detected in a single assay was investigated. the performance of "in-house" separate elisas which use relatively crude l ... | 2011 | 21277304 |
| trim56 is a virus- and interferon-inducible e3 ubiquitin ligase that restricts pestivirus infection. | the tripartite motif (trim) protein family comprises more than 60 members that have diverse functions in various biological processes. although a small number of trim proteins have been shown to regulate innate immunity, much remains to be learned about the functions of the majority of the trim proteins. here we identify trim56 as a cellular protein associated with the n-terminal protease (n(pro)) of bovine viral diarrhea virus (bvdv), a pestiviral interferon antagonist which degrades interferon ... | 2011 | 21289118 |
| [control of bovine viral diarrhea/mucosal disease in the district of kamenz on a voluntary basis--ways, successes, limitations]. | in 69 dairy and beef herds in the district of kamenz, saxony, with a total number of 21,783 and 89.6% of the district's cattle, a voluntary bvdv eradication protocol was implemented from 2000 to 2007. the aim was to achieve eradication as comprehensive as possible and to prepare the herds for the mandatory eradication program. essential preconditions for the accreditation of a herd as "free of bvd virus" were the antigen test of all cattle and their offspring for 12 months including completeness ... | 2011 | 21309165 |
| serological response of guinea pigs to oily and aqueous inactivated vaccines containing a brazilian isolate of the bovine viral diarrhea virus (bvdv). | bovine viral diarrhea virus (bvdv) is widespread in cattle in brazil and research shows its large antigenic variability. available vaccines are produced with virus strains isolated in other countries and may not be effective. in this study, inactivated vaccines containing the brazilian bvdv-ib ibsp11 isolate were developed and tested on 6 groups of 10 guinea pigs (cavia porcellus). animals in groups a and c received an aqueous vaccine (aluminum hydroxide); b and d groups received an oily vaccine ... | 2011 | 21316721 |
| combination of reverse transcription real-time polymerase chain reaction and antigen capture enzyme-linked immunosorbent assay for the detection of animals persistently infected with bovine viral diarrhea virus. | bovine viral diarrhea virus (bvdv) is an economically important pathogen of cattle. a successful control program requires early detection and removal of persistently infected (pi) animals. the objective of the current study was to develop, validate, and apply a cost-effective testing scheme for the detection of bvdv pi animals in exposed herds. pooled samples were screened by using a real-time reverse transcription polymerase chain reaction (real-time rt-pcr), and individual positives were ident ... | 2011 | 21217023 |
| comparison of detection of bovine virus diarrhea virus antigen in various types of tissue and fluid samples collected from persistently infected cattle. | bovine viral diarrhea viruses are economically important pathogens of cattle. most infections in susceptible animals are acquired from animals persistently infected with the virus. surveillance programs rely on skin biopsies of persistently infected (pi) cattle to detect the infection. the purpose of this study was to compare antigen capture enzyme-linked immunosorbent assay (ace) testing results using different types of samples from pi animals. the intent was to determine comparative detection ... | 2011 | 21217032 |
| effect of bovine viral diarrhoea virus biotypes on adherence of sperm to oocytes during in-vitro fertilization in cattle. | bovine viral diarrhoea virus (bvdv), a member of the pestivirus genus, is one of the most important pathogens of dairy cattle; it can cause several clinical syndromes, ranging from subclinical to severe disease. the objectives of the current studies were to assess the effects of two biotypes of bvdv on sperm attachment to the zona pellucida (zp) of oocytes and on fertilization rate in bovine in vitro fertilization (ivf). in two experiments, sperm at two concentrations (10⁵ and 10⁶/ml) and oocyte ... | 2011 | 21220161 |
| identification and characterization of the host protein dnajc14 as a broadly active flavivirus replication modulator. | viruses in the flavivirus genus of the flaviviridae family are arthropod-transmitted and contribute to staggering numbers of human infections and significant deaths annually across the globe. to identify cellular factors with antiviral activity against flaviviruses, we screened a cdna library using an iterative approach. we identified a mammalian hsp40 chaperone protein (dnajc14) that when overexpressed was able to mediate protection from yellow fever virus (yfv)-induced cell death. further stud ... | 2011 | 21249176 |
| survey on management practices related to the prevention and control of bovine viral diarrhea virus on dairy farms in indiana, united states. | the objective of this cross-sectional study was to describe the application of management practices known to be associated with the prevention of bovine viral diarrhea virus (bvdv) infection on indiana dairy farms and to determine the extent of bvdv vaccine use within indiana dairy herds. the population in this study was indiana dairy producers enrolled under the indiana premise id list by the indiana state board of animal health (n=1600). during the fall of 2008 a questionnaire was mailed to in ... | 2011 | 21257213 |
| Establishing a pilot bovine viral diarrhoea virus eradication scheme in Somerset. | Beginning in April 2006, 41 farms were recruited onto a pilot Bovine viral diarrhoea virus (BVDV) eradication programme across the south of England with the majority of study herds concentrated in Somerset. Each herd was assessed and where relevant cleared of persistently infected (PI) animals. Seven farms dropped out before whole herd screening could be performed. Of the remaining 34 farms, 20 (59 per cent) were classified as infected although two of these were initially misclassified as BVDV-f ... | 2011 | 22027186 |
| Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair. | The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three, 1~3 week old, non-bovine viral diarrhea virus (BVDV) vaccinated calves, found to be positive for BVDV by immunohistochemical staining, were selected and hairs were manually plucked from the ear. qRT-PCR was performed on samples consisting of more than 30 hairs (30~100) and whole blood. All 23 animals ... | 2011 | 21897105 |
| use of sentinel serology in a bovine viral diarrhea virus eradication program. | forty-seven cattle management groups from 36 herds in a regional bovine viral diarrhea virus (bvdv) eradication program were selected to evaluate serology as a tool to detect herd infection with bvdv. serum samples were obtained from 5 non-vaccinated sentinel calves ≥ 6 months old in each management group and virus neutralizing (vn) antibody titers against bvdv genotypes 1 and 2 were determined. a herd was considered positive if 2 or more sentinel calves had vn antibody titers ≥ 128 to either ge ... | 2011 | 21908280 |
| Complementation studies with the novel "Bungowannah" virus provide new insights in the compatibility of pestivirus proteins. | In recent years several atypical pestiviruses have been described. Bungowannah virus is the most divergent virus in this group. Therefore, heterologous complementation was used to clarify the phylogenetic relationship and to analyze the exchangeability of genome regions encoding structural proteins. Using a BVDV type 1 backbone, chimeric constructs with substituted envelope proteins E(rns), E1 and E2, were investigated. While all constructs replicated autonomously, infectious high titer chimeric ... | 2011 | 21849202 |
| Molecular Characteristics of Bovine Virus Diarrhoea Virus 1 Isolates from Turkey: Approaches for an Eradication Programme. | Forty pestivirus isolates sampled from cattle in Turkey between 2002 and 2007 were characterized according to 5' untranslated region (5'UTR) sequences and autoprotease (N(pro) ) gene sequences. The sampling of Bovine virus diarrhoea viruses (BVDVs) from 15 farms in five different regions indicated that BVDV 1-l (18/40, 45%) was the predominant genotype in Turkey; the samples also contained the genotypes 1-f (10/40, 25%), 1-b (7/40, 17.5%), 1-d (3/40, 7.5%), and 1-a (2/40, 5%), respectively. | 2011 | 22085446 |
| genetic variation of border disease virus species strains. | the 5´-untranslated region of pestivirus strains isolated from domestic and wild animals were analysed to determine their taxonomic status according to nucleotide changes in the secondary genomic structure using the palindromic nucleotide substitutions (pns) method. a total of 131 isolates out of 536 pestivirus strains evaluated, were clustered as border disease virus (bdv) species. the bdv strains were further divided into at least 8 genotypes or subspecies. thirty-two isolates from small rumin ... | 2011 | 22194225 |
| Development of a set of multiplex standard polymerase chain reaction assays for the identification of infectious agents from aborted bovine clinical samples. | The current study describes the development of a set of 5 multiplex polymerase chain reaction (mPCR) assays for the simultaneous detection of abortive infection agents in bovine fetal tissues, including Brucella spp., Leptospira spp., and Campylobacter fetus (mPCR1); Hammondia heydorni, Neospora caninum, and Toxoplasma gondii (mPCR2); Coxiella burnetii and Chlamydophila psittaci (mPCR3); Mycoplasma bovis, Mycoplasma bovigenitalium, and Ureaplasma diversum (mPCR4); and Bovine viral diarrhea virus ... | 2011 | 21908306 |
| implementation of immunohistochemistry on frozen ear notch tissue samples in diagnosis of bovine viral diarrhea virus in persistently infected cattle. | abstract: | 2011 | 22142412 |
| a nested pcr approach for unambiguous typing of pestiviruses infecting cattle. | an atypical pestivirus ('hobi'-like pestivirus, putative bovine viral diarrhoea 3, bvdv-3) was identified firstly in contaminated foetal calf serum batches and isolated subsequently from an outbreak of respiratory disease in a cattle herd in italy. the isolation of the novel pestivirus from animals affected clinically posed concerns about the validity of bvdv eradication programs, considering that 'hobi'-like pestivirus (bvdv-3) is undetected or mistyped by the molecular diagnostic tools current ... | 2011 | 22119481 |
| an alternative -1/+2 open reading frame exists within viral n(pro)(1-19) region of bovine viral diarrhea virus sd-1. | we previously reported the engineering of an n(pro)-disrupted bovine viral diarrhea virus (bvdv), bsd1-n(pro)/egfp2a (fan and bird, 2008a). here, we report that bsd1-n(pro)/egfp2a survives a single nucleotide missing in its c-terminal egfp region. by using our established reverse genetics system for bvdv, we confirm that the viral mutant is rescued through a -1/+2 orf initiated in the n(pro)(1-19)/egfp region of the mutant viral genome. we furthermore uncover that this event occurs in the n(pro) ... | 2011 | 22079882 |
| response of proinflammatory and anti-inflammatory cytokines in calves with subclinical bovine viral diarrhea challenged with bovine herpesvirus-1. | the aim of this work was to investigate the susceptibility of calves infected with bovine viral diarrhea virus (bvdv) against secondary infections. for this purpose, the profile of cytokines implicated in the immune response of calves experimentally infected with a non-cytopathic strain of bvdv type-1 and challenged with bovine herpesvirus 1.1 (bhv-1.1) was evaluated in comparison with healthy animals challenged only with bhv-1.1. the immune response was measured by serum concentrations of cytok ... | 2011 | 21856021 |
| prevalence study of bovine viral diarrhea virus by evaluation of antigen capture elisa and rt-pcr assay in bovine, ovine, caprine, buffalo and camel aborted fetuses in iran. | abstract: bovine viral diarrhea virus is a pestivirus in the family flaviviridae that cause abortions and stillbirths in livestock and its traditional diagnosis is based on cell culture and virus neutralization test. in this study, for more sensitive, specific detection and determined the prevalence of virus in aborted bovine, ovine, caprine, buffalo and camel fetuses the antigen capture elisa and rt-pcr were recommended. from the total of 2173 aborted fetuses, 347 (15.96%) and 402 (18.49%) were ... | 2011 | 22018096 |
| antiviral treatment of calves persistently infected with bovine viral diarrhoea virus. | background: animals persistently infected (pi) with bovine viral diarrhoea virus (bvdv) are a key source of viral propagation within and among herds. currently, no specific therapy exists to treat pi animals. the purpose of this research was to initiate evaluation of the pharmacokinetic and safety data of a novel antiviral agent in bvdv-free calves and to assess the antiviral efficacy of the same agent in pi calves. methods: one bvdv-free calf was treated with 2-(2-benzimidazolyl)-5-[4-(2-imidaz ... | 2011 | 22182713 |
| association of herd bhv-1 seroprevalence with respiratory disease in youngstock in estonian dairy cattle. | the associations between herd bovine herpesvirus 1 (bhv-1) seroprevalence, along with other infectious and farm management factors with bovine respiratory disease (brd) in dairy calves and heifers, were investigated. serum samples from 103 dairy cattle herds were analyzed for antibodies against bhv-1, bovine respiratory syncytial virus (brsv), bovine viral diarrhea virus (bvdv), and mycoplasma bovis (m. bovis). a questionnaire was used to record herd management practices. a high occurrence of re ... | 2011 | 22100246 |
| bovine viral diarrhea virus nonstructural protein 5a is phosphorylated by cdc2 cyclin-dependent kinase in vitro. | bovine viral diarrhea virus (bvdv) is an enveloped, positive-stranded rna virus that belongs to the genus pestivirus within the family flaviviridae. bvdv nonstructural protein 5a (ns5a) is a 56-58-kda phosphorylated zinc metalloprotein and an essential component of the bvdv replicase complex. an earlier report suggested that ns5a is phosphorylated by a serine/threonine protein kinase in bvdv-infected cells; however, the identity of the protein kinase remained unknown. to determine which protein ... | 2011 | 22167250 |
| spatial and temporal reconstruction of bovine viral diarrhea virus genotype 1 dispersion in italy. | bovine viral diarrhea virus (bvdv) is a widespread and economically important pathogen of cattle; genetic typing of bvdv isolates distinguished two species, namely bvdv-1 and bvdv-2. bvdv-1 is the most widespread worldwide and it includes at least 11 subtypes. with the aim of clarifying the routes of circulation of bvdv-1 subtypes in an endemic area and in order to investigate the relationships between the genetic diversity of bvdv and its geographic distribution, a phylogenetic analysis of 5' u ... | 2011 | 22210133 |
| Inactivation at various temperatures of bovine viral diarrhea virus in beef derived from persistently infected cattle. | Bovine viral diarrhea virus (BVDV) is a pestivirus that is enzootic in most cattle populations throughout the world. This virus is present throughout the body of persistently infected (PI) cattle. Previous research has not assessed the cooking temperature at which BVDV in meat from PI cattle can be inactivated. Therefore, muscle tissue from six PI cattle was harvested, refrigerated, frozen and heated to various internal temperatures. The concentration of virus present was determined by virus iso ... | 2011 | 21965453 |
| [bovine neonatal pancytopenia in german holstein calves]. | profiles of blood cell counts were evaluated for 15 calves from three different farms. these calves showed petechia in the mucous membranes and in the skin and prolonged secondary bleeding after puncture. the clinical course of the disease could be observed in eleven calves. with exception of one case, the blood cell counts indicated a severe anaemia, leukocytopenia and thrombocytopenia. out of these 15 calves, six calves survived and the other nine calves died or had to be euthanized due to the ... | 2011 | 21848041 |
| Validation of model virus removal and inactivation capacity of an erythropoietin purification process. | Human erythropoietin (hEpo) production requires mammalian cells able to make complex post-translational modifications to guaranty its biological activity. As mammalian cell can be reservoir of pathogenic viruses and several animal origin components are usually used in the cultivation of mammalian cells, hEpo contamination with viruses is something of great concern. As consequence, this study investigated the viral removal and inactivation capacity of a recombinant-hEpo (rec-hEpo) purification pr ... | 2011 | 21982851 |
| development of an indirect immunofluorescence assay for diagnosis of bovine viral diarrhoea virus on ear notch tissue samples in cattle infected persistently. | bovine viral diarrhoea virus (bvdv) causes a disease that has a wide range of clinical symptoms in domestic and wild ruminants. it is a major problem in cattle and causes significant economic losses in the cattle industry. the virus infects bovines of all ages and causes both immunosuppression and reproductive, respiratory and digestive disorders. cattle infected persistently, as a continuing source of the virus and the main factor in transmission of the disease between and among herds, are the ... | 2011 | 21875618 |
| epidemiology of pestivirus infection in wild ungulates of the french south alps. | inter-species transmission is often incriminated in the epidemiology of pestivirus diseases. the purpose of this study was to investigate the prevalence of pestivirus in some mountain wild ungulates and to determine their role in pestivirus transmission, as mountain pastures are a place where cohabitations between wild and domestic ungulates are particularly high. between 2003 and 2007, a longitudinal epidemiological study was carried out on hunted ungulates in the french hautes-alpes department ... | 2011 | 20709472 |
| Pestivirus and alphaherpesvirus infections in Swedish reindeer (Rangifer tarandus tarandus L.). | Herding semi-domesticated reindeer has economic and social value for Sami people in the northern territories of Fennoscandia. However, with the intensification of reindeer husbandry, interspecies transmission of pathogens between reindeer and domestic animals may become a problem, especially for countries such as Sweden, Norway, and Finland where pestivirus and alphaherpesvirus have been eradicated in domestic ruminants. This study, which included 1158 Swedish reindeer, showed relatively high pr ... | 2011 | 22078277 |
| effect of injectable trace minerals on the humoral immune response to multivalent vaccine administration in beef calves. | the objective of this experiment was to investigate the effects of injectable trace minerals on humoral responses of calves receiving a viral vaccination. beef steer calves (n = 99; average bw = 316 ± 4.2 kg), seronegative for bovine herpesvirus-1 (bhv-1) and bovine viral diarrhea virus, genotypes 1 and 2 (bvdv-1 and bvdv-2) were sourced from 2 locations. all calves, except 15 non-vaccinated (sentinel) calves, received a single dose of a multivalent modified live vaccine (titanium 5; agrilabs, s ... | 2011 | 22178855 |
| development, optimization, and validation of a classical swine fever virus real-time reverse transcription polymerase chain reaction assay. | classical swine fever (csf) is an economically devastating disease of pigs. instrumental to the control of csf is a well-characterized assay that can deliver a rapid, accurate diagnosis prior to the onset of clinical signs. a real-time fluorogenic-probe hydrolysis (taqman) reverse transcription polymerase chain reaction (rt-pcr) for csf was developed by the united states department of agriculture (usda) at the plum island animal disease center (csf piadc assay) and evaluated for analytical and d ... | 2011 | 21908362 |
| Small interfering RNAs targeting viral structural envelope protein genes and the 5?-UTR inhibit replication of bovine viral diarrhea virus in MDBK cells. | Bovine viral diarrhea viruses (BVDVs) are important pathogens of cattle that occur worldwide, and for which no antiviral therapy is available. In the present study, the inhibitory effect of small interfering (si) RNAs on bovine viral diarrhea virus 1 (BVDV-1) replication in cultured bovine cells was explored. Four synthetic siRNAs were designed to target structural envelope region genes (Erns, E1, and E2) and one cocktail of siRNA was generated to target the 5?-UTR of the BVDV-1 genome. The inhi ... | 2011 | 21978163 |
| vaccine-induced antibodies linked to bovine neonatal pancytopenia (bnp) recognize cattle major histocompatibility complex class i (mhc i). | abstract: a mysterious disease affecting calves, named bovine neonatal pancytopenia (bnp), emerged in 2007 in several european countries. epidemiological studies revealed a connection between bnp and vaccination with an inactivated vaccine against bovine virus diarrhea (bvd). alloantibodies reacting with blood leukocytes of calves were detected in serum and colostrum of dams, which have given birth to calves affected by bnp. to understand the linkage between vaccination and the development of al ... | 2011 | 21878124 |