Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| ebv-specific humoral antibodies in nasopharyngeal carcinoma patients in cuba. | sera of 24 patients with nasopharyngeal carcinoma (npc) and of 60 healthy donors were studied for presence of igg and iga antibodies against different antigens induced by epstein--barr virus (ebv). the results obtained demonstrate that npc in cuba, as in other countries, is accompanied by elevated antibody titers against ebv-specific igg antibodies, whose levels strongly differ from those of healthy persons. patients with npc in cuba had higher titers of antibody against vca of ebv in comparison ... | 1979 | 224330 |
| differential effect of phosphonoacetic acid on early antigen synthesis in two epstein-barr virus producer cell lines. | 1979 | 217163 | |
| prolonged survival in chronic lymphocytic leukemia: a case report. | a patient with long-standing chronic lymphocytic leukemia with both humoral and cellular immunodeficiency had lymph node receptor evidence of a b lymphocyte disorder. he was also found to secrete the epstein--barr virus and, late in his illness, developed a markedly positive antinuclear antibody. interrelationship of these findings may be important in the ultimate determination of the etiology and functional mechanisms in lymphocyte malignancies. | 1979 | 220516 |
| induction of the epstein-barr virus (ebv) cycle in latently infected cells by n-butyrate. | 1979 | 220786 | |
| a possible function of epstein-barr virus-determined nuclear antigen (ebna): stimulation of chromatin template activity in vitro. | 1979 | 220793 | |
| [antiviral activity of interferon and its inducers in human lymphoblastoid and somatic cells]. | the antiviral effect of interferon inductors, such as poly-i--poly-c, phage f2 rna replicative form and low molecular inductor gsn and their influence on cellular dna synthesis were studied in the cultures of lymphoblastoid (inplanting lines raji namalva) and somatic human cells. the semliki forest virus used as the test organism multiplicated well in cells raji accumulating up to 9 lg bou/ml. the two-strand rna was less active in the lymphoid cells than in the somatic ones. gsn was 10 times mor ... | 1979 | 220908 |
| retrovirus-like particles in ebv-negative burkitt's lymphoma cell line but not in ebv-dna-positive lines from patients with ataxia telangiectasia and down's syndrome. | retrovirus-like particles can be recovered by arginine deprivation from the bjab-1 epstein-barr virus (ebv) negative cell line derived from an african patient with typical burkitt's lymphoma. these particles resemble retroviruses in their morphology and in their physicochemical properties. particles with a similar morphology were obtained from derivative cell lines established by infecting bjab-1 cells with ebv. on the other hand, retrovirus-like particles could not be induced in ebv-dna-positiv ... | 1979 | 220999 |
| chromosome aberrations in b lymphocytes of atomic bomb survivors. | although chromosome aberrations in t lymphocytes and bone marrow cells have been reported in atomic bomb survivors, the presence of chromosome abnormalities has not been demonstrated in b lymphocytes because of the technical difficulties involved in b-lymphocyte separation. a method for detecting chromosome aberrations in b lymphocytes was established by "stimulation" of b lymphocytes with epstein-barr virus (ebv) instead of "separation" of b lymphocytes by rosette formation. the ebv-stimulated ... | 1979 | 221056 |
| in vitro growth of cutaneous t-cell lymphomas. | it is relatively easy to propagate eb virus-transformed normal and malignant b lymphocytes in vitro. normal t lymphocytes divide for short periods of time after exposure to many mitogens. exposure of normal t lymphocytes to lymphocyte-conditioned medium (lcm) permits them to divide for longer periods of time, but permanent cell lines cannot be established. intial attempts to grow malignant t cells from patients with cutaneous t-cell lymphomas (ctcl) in unsupplemented growth medium resulted in th ... | 1979 | 221120 |
| kawasaki-like disease in a young adult. association with primary epstein-barr virus infection. | 1979 | 214590 | |
| thoughts on kawasaki disease etiology. | 1979 | 214591 | |
| viral exposure and abnormal liver function in haemophilia. | several studies have recently documented the presence of persistently abnormal liver function tests in asymptomatic haemophiliacs. while the aetiology is unknown it is possible that repeated exposure to agents transmitted in blood products may be important. this study has attempted to determine the prevalence of viral exposure and its relationship to liver function in this multitransfused group of individuals. the prevalence of viral antibodies with the exception of antibody to hepatitis b surfa ... | 1979 | 221550 |
| polyclonal immunoglobulin secretion by human b lymphocytes exposed to epstein-barr virus in vitro. | 1979 | 221572 | |
| lymphocyte activation by the tumor-promoting agent 12-o-tetradecanoylphorbol-13-acetate (tpa). | tpa, a highly active tumor-promoting agent, is an effective mitogen for primate peripheral blood lymphocytes. optimal stimulation of human lymphocytes was obtained 4 days after the addition of tpa at a concentration of 7.5 ng/ml. lymphocyte fractionation experiments demonstrated that both t and b cells incorporated 3h-thymidine significantly in response to tpa. lymphocyte blastogenesis was not due to the reactivation of latent herpesviruses by the tumor promoter, since similar responses to tpa w ... | 1979 | 221578 |
| the effects of mitogens on the expression of epstein-barr virus antigens in human lymphoid cell lines. | treatment of human lymphoblastoid cells with either phytohemagglutinin (pha), concanavalin a, staphylococcus protein a, or polyinosinic acid-polycytidylic acid, in combination with 5-iodo-2' deoxyuridine (iudr) markedly increased the expression of epstein-barr virus (ebv) early antigen (ea) relative to iudr alone. such treatment did not, however, modify the production of virus capsid antigen in any of the lymphoid cell lines tested. the effect of pha on ea induction in raji cells was not accompa ... | 1979 | 221589 |
| cross-neutralization of infectious mononucleosis and burkitt lymphoma strains of epstein-barr virus with hyperimmune rabbit antisera. | 1979 | 221591 | |
| incidence of heterophil antibody responses in children with infectious mononucleosis. | 1979 | 221629 | |
| the epstein-barr virus and eb virus infections in childhood. | 1979 | 221638 | |
| [establishment of leukaemia cell lines and their significance for clinical and experimental oncology (author's transl)]. | permanent in vitro growing leukemic cell lines have been established from all types of immunologically classified childhood leukemias. essential characteristics of primary blasts and cultured cells are identical. in contrast to lymphoblastoid, non-leukemic cell lines, the epstein-barr-virus specific nuclear angiten (ebna) is not detected. up to now 8 non-b-non-t cell lines (6 of them were derived from children with acute lymphoblastic leukemia, 2 from patients with chronic myeloid leukemia), 8 t ... | 1979 | 221724 |
| tumour promoter tpa enhances transformation of human leukocytes by epstein-barr virus. | 1979 | 221841 | |
| practical approach to diagnosis of infectious mononucleosis. | 1979 | 221896 | |
| detection of a nuclear antigen in herpesvirus ateles-carrying marmoset lines by the acid-fixed nuclear binding technique. | in vitro binding of a herpesvirus ateles (hva)-associated soluble antigen to amphibian erythrocyte nuclei was demonstrated by the acid-fixed nuclear binding technique in combination with anticomplement immunofluorescence. incubation of concentrated salt-extracted soluble antigens derived from hva-carrying marmoset lines with methanol/acetic acid-fixed erythrocytes of frogs and salamanders resulted in a brilliant nuclear fluorescence after exposure to a live virus-boostered, anti-hvs-positive squ ... | 1979 | 221913 |
| lymphoma development in mice and humans: diversity of initiation is followed by convergent cytogenetic evolution. | human b cell lymphoma and murine t cell leukemia can be initiated by several agents. the present paper formulates some thoughts on the role of cytogenetic changes in the subsequent neoplastic process. initiation creates long-lived preneoplastic cells. in some respects, they are comparable to in vitro-transformed ("immortalized") cell lines that maintain a diploid karyotype and are not tumorigenic in vivo. the development of a tumorigenic ("autonomous") clone is dependent on additional changes at ... | 1979 | 221925 |
| the epstein-barr virus. | 1979 | 221971 | |
| alterations in cell characteristics in relation to malignant transformation. | 1979 | 222016 | |
| persistence, reactivation, and cell transformation by human herpeviruses: herpes simplex 1, 2 (hsv-1, hsv-2), cytomegalovirus (cmv), varicella-zoster (vzv), epstein-barr virus (ebv). | 1979 | 222414 | |
| the ebv-carrying, beta2m/hla deficient burkitt lymphoma line daudi is sensitive to ebv-specific killer t-cells of mononucleosis patients. | the beta2-microglobulin/hla deficient burkitt lymphoma line daudi was tested for sensitivity to ebv-specific cytotoxicity mediated by natural killer (nk)-depleted t-cells from acute mononucleosis patients. while the daudi line was not as sensitive as the reference ebv-genome-positive target line, it was clearly sensitive in the majority of cases. this would speak against a major role of syngeneic restriction in this system. | 1979 | 222436 |
| epstein-barr virus rna in burkitt tumor tissue. | 1979 | 222455 | |
| induction of plaque-forming cells in human blood lymphocytes cultured in the presence of antigen and epstein-barr virus: a study with normal donors and infectious mononucleosis patients. | normal human peripheral blood lymphocytes, stimulated in vitro with srbc in the presence of epstein-barr virus (ebv), gave rise to plaque-forming cells (pfc) specific for the antigen. pfc levels were very low before day 4 and increased thereafter, reaching a maximum around day 8. however, the kinetics of the response varied considerably from donor to donor and from experiment to experiment. in some instances a second peak of pfc was obtained beyond day 10. large differences in the magnitude of t ... | 1979 | 222519 |
| development of antibodies reactive in antibody-dependent cellular cytotoxicity in infectious mononucleosis. | serial sera from patients with infectious mononucleosis were examined for the emergence of antibodies reactive in antibody-dependent cellular cytotoxicity tests, using epstein-barr virus-superinfected raji cells as targets. for this specific purpose, the antibody-dependent cellular cytotoxicity test proved to be of limited sensitivity because only relatively high serum dilutions can be tested dependably, due to prozone effects at low serum concentrations, and because antibody-dependent cellular ... | 1979 | 222678 |
| persistent infection of a human lymphoblastoid cell line with equine herpesvirus 1. | infection of a human lymphoblastoid cell line (jijoye line derived from a burkitt lymphoma which contains epstein-barr virus) with equine herpesvirus 1, maintained and observed for 53 days, was characterized by the continuous production of infectious extracellular and intracellular virus. maximum virus production correlated with active cell multiplication. less than 15% of the cells possessed viral capsid antigen at any one time. five percent of the cells in the jijoye line possess epstein-barr ... | 1979 | 222688 |
| long-term c-cell-mediated immunity to epstein-barr virus in man. ii. components necessary for regression in virus-infected leukocyte cultures. | regression of eb-virus-induced transformation occurs exclusively in cultures of leukocytes from seropositive donors. studies have shown that the strength of regression could be assayed in terms of the proportion of t cells which must be added to the autologous eb virus-infected t-cell-depleted population in order to establish regression in the culture. the in vitro regression phenomenon was strongly t-cell-dependent but did not require the presence of either monocytes or, as a potential antigeni ... | 1979 | 222690 |
| long-term t-cell-mediated immunity to epstein-barr virus in man. iii. activation of cytotoxic t cells in virus-infected leukocyte cultures. | experiments have been conducted to determine the role played by immune t cells in the regression of eb-virus-induced transformation which is exclusively seen in leukocyte cultures from sero-positive donors. kinetic studies suggest that, in virus-infected cultures from such donors, a population of t cells proliferates within the first 2 weeks apparently in response to the appearance of virus-infected b cells. this proliferation continues to some extent during the period of regression. nonspecific ... | 1979 | 222691 |
| [herpesviruses in man]. | the importance and the extent of the inter-actions between herpes viruses and man is beginning to be understood. as more is learned about the natural histories of these viruses, it is becoming apparent that they have in common: horizontal spread during childhood, often with subclinical infection. latency in the host for many years, probably for life. potential for reactivation at a later time. host immune response maintained for life. potential for oncogeneiss. a more thorough knowledge of the b ... | 1979 | 222711 |
| pleocytosis and immunoglobulin changes in cerebrospinal fluid and herpesvirus serology in patients with guillain-barré syndrome. | in a follow-up study of 24 patients with guillain-barré syndrome, 55% developed a cerebrospinal fluid (csf) mononuclear pleocytosis, which persisted for 4 months or more. raised index values of csf-immunoglobulin g (igg), csf-iga, and csf-igm, indicating synthesis of the immunoglobulin in question in the central nervous system, were found in 63, 35, and 25%, respectively. agarose gel eletrophoresis revealed oligoclonal immunoglobulin in csf in 21% and in both csf and serum in another 21% of the ... | 1979 | 222797 |
| simultaneous radioimmunoassay for specific antibodies to members of the human herpesvirus group. | a method of described for the simultaneous radioimmunoassay (ria) for antibody to members of the human herpesvirus group. the ria is compared with some of the conventional serologic techniques used to quantitate antibody to these viruses (epstein-barr virus, cytomegalovirus, herpesvirus type 1 and varicella-zoster virus). color-coded beads, each coated with the antigens of a different herpesvirus, were similtaneously placed in a well which contained a human serum to be assayed for antibody to ea ... | 1979 | 222846 |
| [eb virus and lymphocytes]. | 1979 | 222920 | |
| theoretical mechanisms for synthesis of carcinogen-induced embryonic proteins: iv. the viruses. | speculations are developed for a mechanism by which oncogenic viruses can induce alterations in cells allowing them to express embryonic genes. it is suggested that if viral deoxyribonucleic acid, directly or via ribonucleic acid directed deoxyribonucleic acid polymerase activity becomes inserted at particular euchromatin - heterochromatin junctions of quasidifferentiated stem-like cells, then deheterochromatization may result, causing in turn derepression of genes for acidic protein phosphokina ... | 1979 | 223021 |
| [the role of herpes and papilloma viruses in human tumors]. | 1979 | 223047 | |
| mitogenic stimulation of leukemia cells by epstein-barr virus. | 1979 | 223056 | |
| long regions of single-stranded dna in human cells. | 1979 | 223063 | |
| infectious mononucleosis. | 1979 | 223111 | |
| sites of sequence variability in epstein-barr virus dna from different sources. | the intracellular epstein-barr virus (ebv) dna present in virus-transformed cells was partly purified from 23 cell lines or biopsies of burkitt lymphoma, nasopharyngeal carcinoma, infectious mononucleosis, or healthy carrier origin. such dna was cleaved in fragments (a-k) of molecular weights between 1 x 10(6) and 30 x 10(6) with restriction enzyme ecori, and these fragments were analyzed by standard methods involving agarose gel electrophoresis, transfer to nitrocellulose filters, and hybridiza ... | 1979 | 223159 |
| a live human b-cell activator operating in isolation of other cellular influences. | using a reversed haemolytic plaque assay, we demonstrate that live but not inactivated epstein-barr (eb) virus can induce human blood lymphocytes to polyclonal ig (igm and igg) secretion in vitro. unlike pokeweed mitogen this b-cell-activating effect takes place in relative absence of t cells. eb virus thus promises to be a tool for study of b cell function isolated from other cellular influences, although its mechanism of activation appears to be different from that of previously known b cell a ... | 1979 | 223232 |
| recovery of epstein-barr virus from nonproducer neonatal human lymphoid cell transformants. | 1979 | 223286 | |
| the establishment of the suspension epstein-barr virus producing cell lines from patients with tumoral diseases. | isolation of the epstein-barr virus (ebv) in suspension lymphoblastoid cell lines from human patients with tumor diseases, mainly malignant lymphoma, has been described. it has been shown that the ebv was isolated from human patients with myeloid type of leukemia in 75% of cases. a similar virus was also isolated from patients with hodgkin's disease and leukemoid reaction of the myeloid type for lung cancer. morphological, cytochemical, immunological, and cytogenetic characteristics of the cell ... | 1979 | 223867 |
| expression of differentiated functions in hybrid cells. | 1979 | 223931 | |
| [studies on epstein barr virus (ebv)-related antigens: biochemical characterization and purification of ebv-determined nuclear antigen (ebna) (author's transl)]. | 1979 | 223952 | |
| triplex gene dosage effect of tpi and g3pd in a human lymphoblastoid cell line with partial trisomy 12p13 and 18p. | the ebv-induced lymphoblastoid line established from a patient carrying a duplication of the distal part of chromosome 12 short arm (12p13) retained the original partial trisomy and displayed the same triplex gene dosage effect for tpi and g3pd as found in the patient's rbc and wbc. | 1979 | 223966 |
| simultaneous presence of ebna-positive and colony-forming cells in peripheral blood of patients with infectious mononucleosis. | ebna-positive lymphoblast cells were detected in 0.1 to 0.9% of the t-cell-depleted lymphocytes obtained from peripheral blood samples of five patients with infectious mononucleosis (im). the same blood specimens from four of the five patients contained cells that formed ebna-positive colonies in soft agar containing ebv antibodies. the ratio of the colony formers to ebna-positive cells was higher in blood samples taken early in the disease than in those obtained in later stages of the disease. ... | 1979 | 223990 |
| relationship between the epstein-barr virus genome and nasopharyngeal carcinoma in caucasian patients. | in order to explore whether undifferentiated nasopharyngeal carcinoma (npc) shows a regular association with epstein-barr virus (ebv), regardless of the geographical and ethnic origin of the patient, a correlated histopathological and nucleic acid hybridization study was performed on biopsies from caucasian patients with nasopharyngeal carcinomas and from various controls. among 12 undifferentiated npcs, 11 were positive for ebv-dna, with multiple copies of the viral genome per cell. serological ... | 1979 | 223991 |
| light and electron microscopic investigations of nasopharyngeal carcinomas with regard to the viral etiology of these tumors. | five carcinomata of the nasopharynx (four lymphoepithelial carcinomata of the regaud type and one squamous cell carcinoma) were examined light and electron microscopically. in addition to the familiar histological and cytological features of these tumors, and because of an increased antibody titer against epstein-barr virus in all five patients, all those cytoplasmic and nuclear inclusions were examined which could be interpreted as indicative of a virus contact. the following structures were fo ... | 1979 | 224065 |
| [c3 receptor expression in lymphocytes]. | 1979 | 224224 | |
| burkitt's lymphoma in iraq. clinical and pathological study of forty-seven patients. | the clinical and pathological features of 47 patients with burkitt's lymphoma seen at the university of baghdad teaching hospital over the years 1969-1977 are described. there were 30 males and 17 females with a mean age of 7.5 years. all patients presented with advanced disease; 44 were in stage iii and 3 in stage iv, (1 with central nervous system involvement; 1 with bone-marrow involvement; 1 presented as acute leukaemia). the abdomen, with or without other sites, was involved in 42 patients. ... | 1979 | 215559 |
| 14q+ marker chromosome in an ebv-genome-negative lymph node without signs of malignancy in a patient with ebv-genome-positive nasopharyngeal carcinoma. | in a patient with an ebv-genome-positive nasopharyngeal carcinoma, an ebv-genome-negative inguinal lymph node without histological evidence of malignant lymphoma or metastatic carcinoma growth was found to contain a 14q+ marker chromosome, identified as an 8;14 translocation, in all cells analyzed. this observation indicates that chromosome aberrations may precede histological signs of malignancy. the possible implication of this finding in relation to the postulated role of the 14q+ marker and ... | 1979 | 215561 |
| correlated expression of a b-lymphocyte-specific glycoprotein (gp27/35) and the ebv receptor/c3 receptor complex in sublines from the same burkitt lymphoma. | a series of virus-producer and non-producer sublines, derived from the burkitt lymphoma line jijoye and its p3hr-1 clone, were previously found to differ in the expression of ebv receptors and, in parallel, c3 receptors. the differences could be related to an "internal selection" caused by the cytopathic p3hr-1 virus variant, favouring the growth of receptor-negative cells. we have now analyzed the same lines for the expression of a b-lymphocyte-specific glycoprotein (gp27/35). a close parallell ... | 1979 | 215562 |
| cell-density-dependence for growth in agarose of two human lymphoma lines and its decrease after epstein-barr virus conversion. | we have compared the growth in agarose medium of two ebv-negative burkitt lymphoma lines, bjab and ramos, and that of their ebv-converted derivatives. optimal conditions for growth in agarose medium are described. the original ebv-negative lines can grow in agarose to a high efficiency, provided a critical level of cell concentration is attained. below this level, there is no growth at all. ebv-converted sublines form colonies at a much lower cell concentration, and their maximal plating efficie ... | 1979 | 215564 |
| biochemical identification of primate lymphoid cell-surface glycoproteins. | we have employed the galactose oxidase-tritiated sodium borohydride labelling method to examine the surface glycoproteins of cotton-topped marmoset and other primate cell lines either established from tumors or transformed in vitro by different lymphotropic herpesviruses. the labelled surface glycoproteins were separated on acrylamide gels in the presence of sodium dodecyl sulfate (sds) and analyzed by fluorography. our results indicate that (1) lymphocytes of the same class from different prima ... | 1979 | 215565 |
| susceptibility of lymphoblastoid cells to infection with human cytomegalovirus. | human lymphoblastoid cells of b- and t-cell origin were examined for their in vitro susceptibility to infection with human cytomegalovirus (cmv). results of infectious-center assays, at virus to cell ratios of 10, indicated that in each of the lymphoblastoid cell lines tested less than 1% of the cells produced infectious virus. under these conditions, cmv specific antigens were undetectable. infection of lymphoblastoid cells with cmv resulted in atypical virus growth curves similar to those obta ... | 1979 | 217829 |
| [clinical significance of sensitive and specific variations of the paul-bunnell reaction]. | 1979 | 218156 | |
| tumor initiators and promoters in the induction of epstein-barr virus. | the effect of various tumor initiators and promoters on induction of persisting epstein-barr virus (ebv) in different lines of lymphoblastoid cells was analyzed. neither five polycyclic aromatic hydrocarbons, amongst them potent tumor initiators (e.g., 7,12-dimethylbenz[a]anthracene), nor the potent (ultimate) liver carcinogen n-acetoxy-n-2-acetylamino-fluorene induced ebv. a series of compounds, representing three classes of tumor-promoting diterpene esters (e.g., 12-o-tetradecanoylphorbol-13-a ... | 1979 | 218219 |
| cytomegalovirus granulomatous hepatitis. | three patients with granulomatous hepatitis due to cytomegalovirus are described. they are compared to the three previously described patients with this disease, and their clinical and serologic characteristics are discussed. similarities and differences between infectious mononucleosis (epstein-barr virus) and cytomegalovirus infections are adduced. that cytomegalovirus may be a cause of granulomatous hepatitis in the adult is stressed. | 1979 | 218449 |
| simultaneous infection with multiple herpesviruses. | 1979 | 218450 | |
| effects of nucleoside analogs on epstein-barr virus-induced transformation of human umbilical cord leukocytes and epstein-barr virus expression in transformed cells. | two methods of assay, measuring (i) stimulation of host cell dna synthesis by [(3)h]thymidine incorporation and (ii) morphological transformation in microtiter plates, were employed to determine what effect treatment during infection with adenine arabinoside or 5-iodo-5'-amino-2',5'-dideoxyuridine has on epstein-barr virus (ebv) (b95-8)-induced transformation of human umbilical cord leukocytes. it was found that adenine arabinoside inhibited ebv-induced transformation in a dose-dependent manner ... | 1979 | 218497 |
| hla-d locus typing in ankylosing spondylitis and reiter's syndrome. | hla-d typing of 44 patients with ankylosing spondylitis (as) and 31 patients with reiter's syndrome (rs) did not show increased frequency of any particular dw allele in either population of patients as compared to controls. such studies also allowed each patient's general response to be compared with other general responses within each experiment. contrary to reports of diminished lymphocyte responses in as patients, hyperresponsiveness in both as and rs patients was found. | 1979 | 218595 |
| request for specimens from organ transplant and immunodeficiency patients who developed lymphomas. | 1979 | 218723 | |
| analysis of the transformation of human lymphocytes by epstein-barr virus. i. sequential occurrence from the virus-determined nuclear antigen synthesis, to blastogenesis, to dna synthesis. | a b-cell population of human cord blood lymphocytes was exposed to the b95-8 strain of epstein-barr virus (ebv), and simultaneous observations of immunofluorescence, cellular morphology and autoradiography were carried out in each individual cell. it was evident that ebv-determined nuclear antigen (ebna) synthesis occurred prior to blastogenic response and dna synthesis. ebna-positive cells could be observed as early as 12 h after infection and reached a maximum of 17% at 24 h, followed by a pla ... | 1979 | 218892 |
| replication of herpes simplex virus in human b lymphocytes stimulated by epstein-barr virus. | human b lymphocytes prepared from adult blood or cord blood by the use of neuraminidase-treated sheep red blood cells which did not respond mitogenically to phytohemagglutinin or pokeweed mitogen, responded well to the b95-8 strain of epstein-barr virus, but not to the p3hr-1 strain, with increased incorporation of tritiated thymidine. replication of herpes simplex virus could be demonstrated in cultures of b lymphocytes preincubated with the b95-8 strain. | 1979 | 218893 |
| sensitive solid-phase radioimmunoassay for detection of human immunoglobulin g antibodies to varicella-zoster virus. | a sensitive solid-phase radioimmunoassay for detection of antibodies to varicella-zoster virus (vzv) is described. the antigen consisted of a sonically disrupted extract of vzv-infected human embryo cells. 125i-labeled rabbit anti-human immunoglobulin g (igg) specific for the fc portion of human igg was used to detect human igg bound to viral antigen. with this technique, 193 human sera were evaluated for their igg antibody titer against zvz. subjects included 62 healthy adults, 33 young childre ... | 1979 | 219015 |
| characterization of an epstein-barr virus-induced dna polymerase. | the addition of iododeoxyuridine to p3hr-i cell cultures led to a large increase in both epstein-barr virus (ebv)-induced dna polymerase activity and early antigen-positive cells. this ebv-induced dna polymerase was separated from the cellular alpha- and beta-polymerases by sequential column chromatography on sepharose 6b, deae-cellulose, and phosphocellulose, resulting in partial purification of about 320-fold. the partially purified-ebv dna polymerase could be distinguished from the cellular d ... | 1979 | 219209 |
| epstein-barr virus-specific rna. iii. mapping of dna encoding viral rna in restringent infection. | namalwa and raji cells, originally obtained from a burkitt tumor biopsy, grow as continuous cell lines in vitro and contain the epstein-barr virus (ebv)-related nuclear antigen ebna (b. m. reedman and g. klein, int. j. cancer 11:499-520, 1973) and rna homologous to at least 17 and 30% of the ebv genome, respectively (s. d. hayward and e. kieff, j. virol. 18:518-525, 1976; t. orellana and e. kieff, j. virol. 22:321-330, 1977). the polyribosomal and polyadenylated [poly(a)+] rna fractions of namal ... | 1979 | 219221 |
| nucleosomal structure of epstein-barr virus dna in transformed cell lines. | micrococcal nuclease digestion was used to analyze epstein-barr virus (ebv) dna structure in nuclei of transformed cells. digests of virus-producing (p3hr-1), non-virus-producing (raji), and superinfected rajii cell nuclei were fractionated by electrophoresis on agarose gels, transferred to nitrocellulose, and hybridized to 32p-labeled ebv dna. the viral dna of raji nuclei produced a series of bands on electrophoresis whose lengths were integral multiples of a unit size, which was the same as th ... | 1979 | 219253 |
| size of the intracellular circular epstein-barr virus dna molecules in infectious mononucleosis-derived human lymphoid cell lines. | the size of non-integrated circular epstein-barr virus (ebv) dna molecules isolated from seven different human lymphoblastoid cell lines of infectious mononucleosis origin has been determined by sedimentation analysis and by direct contour length measurements on electron micrographs. six lines had intracellular circular ebv genomes of the same size as linear virion dna molecules. the seventh line, established with the b95-8 strain of ebv, was the only one found to have circular ebv dna molecules ... | 1979 | 219261 |
| retinoic acid inhibition of epstein-barr virus induction. | 1979 | 219367 | |
| [blood transfusion and viral diseases. recent acquisitions concerning viral hepatitis viruses, cytomegaloviruses and epstein-barr virus]. | in recent years, an increasingly clear picture has been formed of the virus-induced syndromes that may follow a blood transfusion or the use of blood derivatives. up to about 10 years ago, post-infusion infection was predominantly due to serum hepatitis. blumberg's discovery of hbsag (formerly known as australia antigen) has made it possible to check and prevent viral hepatitis, type b, and to recognise such distinct forms as the mononucleosis-like syndrome caused by cytomegalic virus, infectiou ... | 1979 | 219399 |
| epstein-barr virus antibody responses and clinical illness in renal transplant recipients. | among 88 renal transplant recipients evaluated for a change in epstein-barr virus (ebv) antibody status in the period after transplant, 22 showed a 4-fold rise and eight showed an 8-fold or greater rise in ebv antibody. among the patients with an 8-fold or greater ebv antibody rise, the occurrence of fever was frequent, one patient developed a lymphoproliferative reaction, and one died with a malignant ebv infection. patients without pretransplant antibody showed a longer mean time to antibody r ... | 1979 | 219556 |
| viral serology (hepatitis b virus, cytomegalovirus, epstein-barr virus) and abnormal liver function tests in transfused patients with hereditary hemorrhagic diseases. | 1979 | 219564 | |
| establishment of a malignant, epstein-barr-virus (ebv)-negative cell-line from the pleura effusion of a patient with hodgkin's disease. | 1979 | 216443 | |
| detection of antibody to a new antigen induced by epstein-barr virus in rheumatoid arthritis. | a new antigen which is different from epstein-barr virion antigen was detected in nc-37 cells infected with epstein-barr virus (ebv). a significant elevation of the titer of antibody to this new antigen was observed in the sera of the patients with rheumatoid arthritis (ra), but not in the sera of controls. from the standpoint of the etiologic role of ebv in ra, it is interesting that the antibody to the new antigen is not detected in the healthy persons in contrast to other viral antibodies. th ... | 1979 | 216133 |
| infectious mononucleosis and epstein-barr virus. 1. epidemiology, pathogenesis, immune response. | the evidence linking epstein-barr virus causally with infectious mononucleosis is compelling. the disease occurs only in persons who lack antibody to this virus, and serologic findings during the acute illness provide almost certain evidence for its etiologic association. additional evidence is derived from transformation studies using lymphocytes from persons infected with epstein-barr virus. epidemiologic studies have related the need for close contact with salivary secretions to the low commu ... | 1979 | 215976 |
| infectious mononucleosis and epstein-barr virus. 2. clinical picture, diagnosis, management. | the diagnosis of infectious mononucleosis is confirmed by hematologic, biochemical, and serologic data. an absolute increase in peripheral mononuclear cells to at least 4,500/cu mm is an essential feature. the serologic hallmark of the disease is the presence of heterophile antibody. the age of the patient may affect the clinical presentation and laboratory findings. therapy is largely supportive, with steroids being used only in the presence of certain severe complications. | 1979 | 215977 |
| [a specific igm test for the diagnosis of epstein-barr virus infections (author's transl)]. | igm antibodies specific for epstein-barr virus (ebv) were measured in 302 patients with high igg antibody titers to study whether ebv was the cause of disease in children having one or more symptoms of classical infectious mononucleosis. igm antibodies specific for ebv were found in all patients with the defined clinical picture of infectious mononucleosis. in addition the majority of cases with clinical suspicion of the disease had also specific igm titers. besides infectious mononucleosis ebv ... | 1979 | 390371 |
| cultured "hairy cells" infected with epstein-barr virus: evidence for b-lymphocyte origin. | 1979 | 311181 | |
| sister chromatid exchanges and chromosomes in chronic myelogenous leukemia and cancer families. | spontaneous sister chromatid exchanges and banded karyotypes were studied in blood lymphocytes from 96 individuals: seven patients with chronic myelogenous leukemia, 15 normal controls, and five "cancer families" comprising 12 cancer patients, 40 tumor-free blood relatives and 22 spouses. the families had: malignant melanoma; epstein-barr virus-associated malignancies and a birth defect syndrome; non-hodgkin lymphoma and diverse carcinomas; hodgkin's lymphoma and adenocarcinomas; and acute myelo ... | 1979 | 282271 |
| cytogenetic study of a european burkitt's lymphoma cell line. | the chromosomes of an epstein-barr virus-negative european burkitt's lymphoma cell line were studied. all the cells carried the t(8;14) translocation. one clone had 51 chromosomes and was (+1,+7,+16,+15,+21), whereas another clone also had 51 chromosomes but was (+1q+,+7,+16,+15,+21). a third clone had 46 chromosomes (4q+/-). | 1979 | 286095 |
| acute non-a, non-b hepatitis. prolonged presence of the infectious agent in blood. | non-a, non-b hepatitis, previously transmitted to chimpanzees by inoculation of human serum, was serially transmitted through a second and third passage to additional chimpanzees using serum drawn during acute non-a, non-b hepatitis. sera obtained at weeks 4 and 5 after inoculation from two different chimpanzees, and from one chimpanzee at week 13 after inoculation, were shown to cause elevation of serum aminotransferase levels and abnormal liver biopsies in recipient chimpanzees, with no serolo ... | 1979 | 421994 |
| cell-mediated lymphocytotoxicity directed against hla-d gene products. | cytotoxic effector cells were generated in human mixed lymphocyte cultures (mlcs) between cell donors differing at the hla-d/dr region but not at hla-a,b(c). these cells killed epstein-barr virus-infected lymphoblasts (ebv blasts) from donors sharing the hla-d/dr determinants with the normal mlc-stimulating cells, but not phytohaemagglutinin (pha) lymphoblasts from the same donors. because ebv blasts are derived from b cells and express hla-d/dr antigens, the most likely interpretation of these ... | 1979 | 432943 |
| histological and electron microscopic studies of endemic ethmoidal carcinomas in cattle. | in five cases of endemic ethmoidal carcinoma in cattle from the dominican republic three tumor types could be classified: undifferentiated carcinoma (3), adenocarcinoma (1), and squamous cell carcinoma (1). electron microscopy showed that the tumor cells in undifferentiated carcinomas closely resembled the cells of the normal olfactory mucosa. this was especially true for the dark cells of bowman's gland. ultrastructurally, the lymphoid cells of the undifferentiated bovine carcinoma resembled th ... | 1979 | 442448 |
| non-a, non-b hepatitis among participants in a plasmapheresis stimulation program. | non-a, non-b hepatitis was transmitted to seven of nine participants in a red blood cell-stimulation program following transfusion of blood from one asymptomatic donor. five of the seven patients had clinical as well as biochemical evidence of infection, and three of these five were icteric. incubation periods for the clinical cases ranged from 28 to 50 days, and duration of illness was from two to eight weeks. none of the seven patients showed serologic evidence of acute infection or reinfectio ... | 1979 | 448190 |
| association of human chromosome 14 with a ts defect in g1 of chinese hamster k12 cells. | somatic cell hybrids between human lymphoblastoid cells (raji) and temperature-sensitive chinese hamster cells (k12) were selected from monolayer cultures in mem at 40 degrees c. a total of 21 hybrid clones were isolated and karyotyped. all clones contained a near complete set of chinese hamster chromosomes and 1 to 5 human chromosomes. human chromosome 14 present in the hybrid cells of all clones; and was the only human chromosome retained in 10 clones. the presence of human chromosome 14 in hy ... | 1979 | 455493 |
| infectious mononucleosis in the elderly. | infectious mononucleosis (im) was diagnosed in four patients over the age of 50 years. their age, absence of splenomegaly, lack of significant lymphadenopathy in three and an atypical presentation in one all contributed to a delay in the diagnosis. in two patients, in whom complications occurred, the paul-bunnell test was repeatedly negative. confirmation of the diagnosis was made by the measurement of the epstein-barr virus igm using differential sucrose gradient centrifugation. because the pre ... | 1979 | 463681 |
| 2/8 translocation in a japanese burkitt's lymphoma. | a new translocation between chromosomes 2 and 8, t(2p-; 8q+), was found in fresh lymphoma cells from a japanese patient with epstein-barr virus-carrying burkitt's lymphoma, and in a lymphoma cell line derived from this patient. there was no 14q+ translocation, as has been previously described in african and north american burkitt's lymphomas. | 1979 | 467575 |
| remission of giant lymph node hyperplasia with anemia after radiotherapy. | we have described a 51-year-old patient with unresectable mesenteric giant lymph node hyperplasia of the plasma cell type, severe systemic manifestations, and profound anemia. supression of erythropoiesis may have been related to the presence of a circulating erythropoietic inhibitor produced by the lymphoid tumor. markedly elevated titers to epstein-barr virus capsid antigen suggest that this virus may be important in the etiology of the abnormal lymphoid proliferation. the marked clinical resp ... | 1979 | 476562 |
| cell culture studies in progeria. i. establishment and partial characterization of a lymphoblastoid cell line. | a lymphoblastoid cell line (eks-l44) with definitive hla specificities (hla-aw24, w30, b14, w35, cw4-, drw4, 5) was established from peripheral blood cells of a patient with progeria using epstein-barr virus. cytogenetic study indicated that eks-l44 was diploid after four months in vitro. the growth pattern of this cell line was not significantly different from a similarly established normal control nor from other lines initiated from patients with various disorders. | 1979 | 513854 |
| chromosome 14q+ in a japanese patient with burkitt's lymphoma. | cytogenetic studies were performed on a biopsy specimen of a jaw tumor and on a bone marrow aspirate from a japanese patient with epstein-barr virus-negative burkitt's lymphoma. a 14q + chromosome was found in cells from either source, although each contained a different clone. other karyotypic abnormalities present in common included 2dir dup (1q) (q21 leads to q32), 3q+, 6p--, +12, +mar. | 1980 | 6446841 |
| multiple hepatitis attacks in drug addicts. | radioimmunoassays for detection of hepatitis b surface antigen (hbsag), antibody to hbsag (anti-hbs), antibody to hepatitis b core antigen (anti-hbc), antibody to hepatitis a virus (anti-hav), and anti-hav of igm class were used to verify hepatitis a and hepatitis b infection in 33 drug addicts with multiple attacks of hepatitis. hepatitis a was confirmed serologically in 23 (32%) of 71 total hepatitis episodes, while hepatitis b was confirmed in 30 episodes (42%). the remaining 18 hepatitis epi ... | 1980 | 6766507 |
| interaction of herpesvirus ateles and hepesvirus saimiri with primate lymphocytes. i. selective adsorption of virus by lymphoid cells. | a semiquantitative infectious bioassay, described by sairenji and hinuma for the measurement of epstein-barr virus (ebv) receptors, was adapted to study the adsorption of the lymphotropic new world herpesvirus ateles (hva) to established lymphoid lines and lymphocytes of human versus marmoset origin. all marmoset t-cell lines [hva- or hvs (herpesvirus saimiri)-carrying] had a high adsorbing capacity. ebv-carrying marmoset or human b-cell lines, one ebv-negative human b-lymphoma line, and one hum ... | 1980 | 6766914 |
| etiological characterization of hepatitis b surface antigen-negative hepatitis among adult patients in athens, greece. | in a 4-month period, 216 cases of acute viral hepatitis were diagnosed in adults at the infectious diseases hospital, athens, greece. twenty-six percent of these were hepatitis b surface antigen negative. a full set of clinical specimens was obtained from 19 of these patients, who were studied in depth for the etiology of their hepatitis. a total of 7 of the 19 patients had serological evidence of hepatitis a virus infection, and 2 had evidence of recent hepatitis b virus infection. the remainin ... | 1980 | 6769949 |
| [viral hepatitis. occupational hazard for anaesthesists (author's transl)]. | hepatitis has been recognized as an occupational hazard of health professionals for many years. the recent findings concerning the structure of hepatitis a, b, non-a non-b, as well as refined testing procedures have increased the ability and thus the frequency of its diagnosis in occupationally exposed medical personnel. other viruses such as epstein barr virus (ebv) and cytomegalovirus (cmv) can also be involved. among the inferences to be drawn from recent research is that hepatitis b virus ha ... | 1980 | 6775557 |
| prevalence of type b and non-a, non-b hepatitis in hemophilia: relationship to chronic liver disease. | to assess the relationship of liver dysfunction and hepatitis markers in hemophilic patients treated with factor viii or ix concentrates, we studied 103 patients with hemophilia a and b for 6-36 mo. elevated serum alanine aminotransferase was noted in 79% of the patients, with 51% of the patients showing persistent elevation for longer than 6 mo. thirteen patients (12%) were hbsag-positive, with 8 patients showing persistence of hbsag and abnormal serum alanine aminotransferase for more than 6 m ... | 1980 | 6777232 |