Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| genital herpes: natural history and treatment of acute episodes. | genital herpes is a common, distressing infection whose incidence has been underestimated. recent serological surveys, employing type-specific antibody assays, show a rising prevalence of previous herpes simplex virus (hsv) type 2 infections in post-adolescent populations in developed countries; many of these infections have been asymptomatic. in some geographical locations, hsv-1 infections are a common cause of first episodes. they may occur in stable, monogamous relationships and are less lik ... | 1993 | 8245890 |
| herpes simplex virus infections during the decade since the licensure of acyclovir. | the development and use of acyclovir accelerated in the area of the natural history, epidemiology, and biology of herpes simplex viruses. the development of serologic assays that differentiate hsv-1 from hsv-2 demonstrated the worldwide distribution of genital herpes, the role genital ulcers play in facilitating hiv transmission, and the high frequency in which pregnant women acquire hsv infection. the high reactivation rate of hsv-2 infections, the often silent nature of genital herpes, and its ... | 1993 | 8245896 |
| isosteres of the dna polymerase inhibitor aphidicolin as potential antiviral agents against human herpes viruses. | a variety of isosteres of the dna polymerase inhibitor aphidicolin were synthesized as potential antiherpes agents. modeling studies indicated that the bicyclooctane c, d rings of aphidicolin could be replaced by an aromatic moiety while maintaining the spatial arrangement of the hydroxyl group equivalent to the essential c18 hydroxyl group of aphidicolin. of the racemic isosteres synthesized only 13, the compound with the greatest structural similarity to aphidicolin, showed any significant ant ... | 1993 | 8246219 |
| purification and biochemical characterization of the protein kinase encoded by the us3 gene of herpes simplex virus type 2. | post-ribosomal cytoplasmic fractions from vero cells mock-infected or infected with wild-type herpes simplex virus type 2 (hsv-2) or a us3 gene-disrupted mutant of hsv-2 were fractionated with deae-cellulose chromatography, and the peak fraction of the protein kinase which was detectable only in the extract of wild-type virus-infected cells was subjected to successive chromatography. the enzyme was purified more than 1000-fold from the post-ribosomal supernatant, and the final preparation contai ... | 1993 | 8249291 |
| synergistic anti-hsv effect of tumor necrosis factor alpha and interferon gamma in human corneal fibroblasts is associated with interferon beta induction. | hsv-1 (17) replicated to high titer in human corneal fibroblasts (> 10(8) pfu/10(5) cells) following infection at one pfu per 100 cells. pretreatment of the cells for 24 h with 50 u/ml recombinant human tumor necrosis factor alpha (tnf-alpha) or 5 iu/ml of human interferon gamma (ifn-gamma) resulted in only modest reduction (2- to 19-fold) in virus yield. however, when the two cytokines were combined the antiviral effect was dramatically increased. there was > 1000-fold reduction in virus titer ... | 1993 | 8250541 |
| [three cases of herpes simplex virus type 2 myelitis--detection of hsv2 dna in cerebrospinal fluid]. | polymerase chain reaction (pcr) technique has been successfully used to detect herpes simplex virus (hsv) from patients with hsv encephalitis. by pcr assay capable of differentiating hsv1 and 2, we detected hsv 2 dna in cerebrospinal fluid (csf) from patients with hsv myelitis and discussed the clinical findings. three cases of hsv myelitis (a 49-year-old female, two 38- and 44-year-old males) were studied. all cases were characterized by transverse myelopathy of the thoracic cord, and two patie ... | 1993 | 8252822 |
| [herpetic vestibular neuronitis: a hypothesis]. | abundant experimental research has shown the potentially damaging effects that the herpes simplex virus (hsv) may have on peripheral or central nervous pathways. fifty subjects (ages 20-45) with labial herpes virus (hsv-1) and 15 (ages 25-35) with genital herpes virus (hsv-2) were studied through recording spontaneous and optokinetic nystagmus (okn) and using the eye-tracking-test (ett) by means of electronystagmography (eng). recording was carried out during the first two days after vesicular e ... | 1993 | 8256608 |
| the pathogenicity of a us3 protein kinase-deficient mutant of herpes simplex virus type 2 in mice. | we have investigated the pathogenicity of a us3 protein kinase-deficient mutant (l1 br1) of herpes simplex virus type 2 (hsv-2) for 4-week-old icr mice to define the role of the viral protein kinase in virus-host interaction. when mice were intraperitoneally infected with 10(5)pfu of l1 br1, the virus disappeared from the peritoneal cavity by 2 days postinfection and failed to induce any significant histopathological changes in the liver and spleen although viral antigens were occasionally detec ... | 1993 | 8257288 |
| new approaches to the diagnosis of oral soft-tissue disease of viral origin. | molecular biology is changing the face of diagnostic medicine, and infectious diseases of the oral soft tissues are among the targets of these advances in biotechnology. as an illustration of these concepts, a pcr-based detection and typing system for human papillomaviruses (hpvs) will be discussed. a single "consensus" set of oligomeric nucleotide primers can be used to amplify a 571- to 594-base-pair region of the e1 open reading frame of hpv 6, 11, 16, and 18. these hpv types are commonly ass ... | 1993 | 8260010 |
| identification of cytomegalovirus in vitreous using the polymerase chain reaction. | a presumptive clinical diagnosis of cytomegalovirus (cmv) retinitis was made in two patients presenting with atypical ophthalmologic findings. the diagnosis was confirmed by finding specific cmv dna sequences in sampled vitreous fluid using a polymerase chain reaction (pcr) based assay. neither of these patients yielded cmv on culture or pcr of peripheral blood leucocytes, nor was cmv present in tears or urine of either patient. serum antibody titres were stable and did not help in establishing ... | 1993 | 8260155 |
| diagnosis of genital herpes by polymerase chain reaction amplification. | a new polymerase chain reaction (pcr) method employing type-specific primers and probes was applied to 114 clinical specimens obtained from 58 female patients with genital lesions or who had a history of genital herpes. ten and 15 specimens, respectively, were positive for herpes simplex virus (hsv)-1 and hsv-2 by cell culture. all of 10 culture-confirmed hsv-1 cases and 11 of 15 (73%) culture-confirmed hsv-2 cases were identified by pcr. although there were several cases with discrepancy betwee ... | 1993 | 8133806 |
| [investigation on the incidence of genital herpes in different professional groups in qingdao]. | genital herpes is one of 8 legally reportable sexually transmitted diseases (std) in china. using a hsv antigen elisa kit we detected and typed hsv antigen in 1,148 clinical specimens collected from the genital organs (penis, cervix, vagina and vulva) of 446 men and 702 women in qindao and divided into 11 different professional and 2 special groups (patients with cervical cancer and pregnant women). the highest positive rate of hsv antigen was found among long-distance transport drivers (48.0%). ... | 1993 | 8137406 |
| [molecular epidemiologic investigation on hsv infection among married women at childbearing age]. | by pcr and elisa, an investigation on hsv infection of cervix was carried out among 120 married women. the results indicated that the total infection rate was 23.3% (28/120). in hsv infection of cervix, hsv-2 constituted 89.3%. the positive rate of hsv in the cervicitis group and normal control group was 28.9% (26/90) and 6.7% (2/30), respectively, showing a marked difference: the positive rate in the group from 21 to 30 years old was 16.7% (7/43), from 31 to 40 was 34.6% (9/26); the positive ra ... | 1993 | 8143322 |
| activity of the keggin polyoxotungstate pm-19 against herpes simplex virus type 2 infection in immunosuppressed mice: role of peritoneal macrophage activation. | the in vivo antiviral activity of the keggin polyoxotungstate pm-19 [k7(pti2w10o40).6h2o] against herpes simplex virus type 2 (hsv-2) was investigated in mice immunosuppressed by cyclophosphamide (cy). when pm-19 was administered intraperitoneally to immunosuppressed mice for 3 days (once daily) starting at the time of infection, it prevented death due to hsv-2 encephalitis in a dose-dependent manner (10-25 mg/kg). the in vivo anti-hsv-2 activity of pm-19 was superior to that of acyclovir. intra ... | 1993 | 8263499 |
| [detection of herpes simplex virus 1 and 2 (hsv-1 and hsv-2) igg and igm by elisa in cord blood and sera of mothers with pregnancy complications]. | in this study, hsv-1 igg, igm and hsv-2 igg, igm antibodies by elisa in the sera of the mothers who had different kinds of obstetrical problems like abortus, stillbirth, prematurity, postmaturity, intrauterine development retardation and in the newborns' cord sera who had congenital anomalies like anencephaly, cataract and dolichocephaly were investigated. in these mothers hsv-1 igg positivity ratio was 71/73 (97.3%). there was no significant difference in the age group distribution of hsv-1 igg ... | 1993 | 8264443 |
| acyclovir diphosphate dimyristoylglycerol: a phospholipid prodrug with activity against acyclovir-resistant herpes simplex virus. | infection with herpes simplex viruses (hsvs) resistant to treatment with acyclovir (9-[(2-hydroxyethoxy)-methyl]guanine, zovirax) is a growing clinical problem in patients with aids and other immunosuppressed states. most virus isolates resistant to acyclovir are deficient or defective in virally coded thymidine kinase (tk), which converts acyclovir to acyclovir monophosphate in virus-infected cells. to restore acyclovir efficacy, we synthesized acyclovir diphosphate dimyristoylglycerol, an anal ... | 1993 | 8265634 |
| echovirus type 19 and herpes simplex type 2 infection in human placenta tissue explants. | 1. light and electron microscopy have been used to characterize echovirus 19 and herpes simplex type 2 infection of human placenta tissue in vitro. immunofluorescence, autoradiography and virus adsorption were used to determine virus replication in this system. 2. placental tissue was permissive to echovirus 19. trophoblast cells were lysed with liberation of mature virions. however, during the 48-h period of observation, few cells were damaged and the trophoblastic structure was maintained. 3. ... | 1993 | 8268819 |
| production of herpes simplex virus fluorescein labelled typing reagents. | 29 herpes simplex virus monoclonal antibodies (mabs) were produced and shown by indirect immunofluorescence assay (iifa) to be hsv type specific or hsv cross reactive. two hsv-1 and two hsv-2 specific mabs were selected as hsv typing reagents on the basis of their specificity and the fluorescence pattern produced. when directly conjugated to fluorescein both reagents detected and correctly identified hsv isolates previously typed by restriction endonuclease analysis (rea). no cross reactions wer ... | 1993 | 8270652 |
| herpes simplex virus type 2 infection in pregnancy: asymptomatic viral excretion at delivery and seroepidemiologic survey of two socioeconomically distinct populations in são paulo, brazil. | the objective of the present study was to estimate the prevalence of herpes simplex virus type 2 (hsv 2) antibodies in child bearing women of 2 brazilian populations with different socioeconomic status and to determine the risk of neonatal hsv exposure by means of maternal cultures at the onset of labor. the study was conducted at 2 hospitals: a, serving very low income patients and b, serving middle socioeconomic class. 173 participants from group a and 127 from b answered a questionnaire which ... | 1993 | 8278760 |
| quantitative analysis of herpes simplex virus dna in cerebrospinal fluid of children with herpes simplex encephalitis. | herpes simplex virus (hsv) dna in the cerebrospinal fluid (csf) of children with herpes simplex encephalitis (hse) was quantified and typed using the polymerase chain reaction (pcr) assay. during the acute phase, hsv-dna was detected in the csf of 13 patients with hse, including 5 neonates. a restriction profile of the pcr products cleaved with the restriction enzymes xhoi and bglii showed that 2 neonatal samples were hsv-2, and the remainder were hsv-1. the amount of hsv-dna in the initial csf ... | 1993 | 8283180 |
| herpes simplex virus type 2 dna in cerebrospinal fluid of a woman with recurrent meningitis. | 1993 | 8286655 | |
| latent infection with the ms strain of herpes simplex virus type 2 in the mouse following intracerebral inoculation. | intracerebral inoculation of the ms strain of herpes simplex virus type 2 (hsv-2) into mice causes an acute encephalitis associated with multifocal demyelination and necrotizing retinitis. we have studied the distribution of latent virus in mice that had recovered from the acute encephalitis. four weeks or longer after inoculation, hsv-2 could be recovered from the trigeminal ganglia of all mice examined by co-culture of explants in roller tubes. the virus could not be recovered from explants of ... | 1993 | 8289071 |
| [acyclovir-resistant herpes simplex viruses in hiv-infected patients]. | 1993 | 8290931 | |
| use of isotopically chiral [4'-13c]penciclovir and 13c nmr to determine the specificity and absolute configuration of penciclovir phosphate esters formed in hsv-1 and hsv-2 infected cells and by hsv-1-encoded thymidine kinase. | penciclovir is a potent antiherpesvirus agent which is highly selective due to its phosphorylation only in virus infected cells. phosphorylation of one of the hydroxymethyl groups of penciclovir (pcv) creates a chiral centre leading to the possible formation of (r)- and (s)-enantiomers. the absolute configuration and stereospecificity of the pcv-phosphates produced in cells infected with herpes simplex viruses types 1 and 2 (hsv-1 and hsv-2), as well as by hsv-1-encoded thymidine kinase, were de ... | 1993 | 8305285 |
| flow cytometric indirect immunofluorescence assay with high sensitivity and specificity for the detection of antibodies to hsv-1 and hsv-2. | cells infected with hsv-1 or hsv-2 develop viral antigens which can be detected by immunofluorescence. we developed a flow cytometric indirect immunofluorescence assay to detect and quantitate antibodies to hsv-1 and hsv-2 in human sera. results obtained by flow cytometry for detecting antibodies against hsv-1, when compared with results obtained by elisa, showed an index of overall agreement of 100%. the correlation between the antibody titers obtained with each method was found to be highly si ... | 1993 | 8307141 |
| detection of antibodies to herpes simplex virus type 2 with a mammalian cell line expressing glycoprotein gg-2. | the gene (us4) coding for herpes simplex virus type 2 (hsv-2) glycoprotein g (gg-2) was cloned and constitutively expressed in chinese hamster ovary (cho) cells. the expression vector containing the dihydrofolate reductase (dhfr) gene, and the hsv-2 us4 gene under the control of the simian virus 40 early promoter (sv40 ep), was transfected into dhfr-deficient cho cells. the transfected cells were selected and amplified using methotrexate (mtx). to demonstrate that the gg-2 produced in these tran ... | 1993 | 15566716 |
| rapid immunoassay for the detection of genital herpes infection. | we evaluated a new affinity membrane strip test for the diagnosis of herpetic genital infections. test strip results, which are available by immunoassay in 30 min without the need for special equipment, were compared with the results of viral culture. | 1994 | 18475363 |
| cytomegalovirus/herpesvirus and carotid atherosclerosis: the aric study. | ubiquitous viruses such as members of the human herpes virus group, particularly cytomegalovirus (cmv), have been proposed to be clinically important agents in the initiation and progression of atherosclerosis. antibodies to cmv and herpes simplex virus type 1 (hsv1) and type 2 (hsv2) were determined in 340 matched case-control pairs from the atherosclerosis risk in communities (aric) study. cases were defined by b-mode ultrasonography as persons with thickened carotid artery walls consistent wi ... | 1994 | 8308517 |
| sexually transmitted agents and cervical neoplasia in colombia and spain. | case-control studies of cervical intra-epithelial neoplasia grade iii (cin iii) and of invasive cervical cancer were carried out in spain and colombia to assess the relationship between cervical cancer and 6 common sexually transmitted agents (stas). the cin-iii studies included 525 cases and 512 controls matched for age and for the place of recruitment; the invasive-cancer studies included 373 histologically confirmed cases of squamous-cell carcinoma and 387 age-stratified controls selected ran ... | 1994 | 8314322 |
| s-glucosylated hydantoins as new antiviral agents. | s-glycosylation took place on reaction of 5-alkylidene- and 5-arylidene-3-aryl-2-thiohydantoins with glycosyl halides under alkaline conditions. bisglucosylation also took place when n-3 unsubstituted hydantoins were reacted. the bisglucosylated hydantoins produced n-3 glucosylated hydantoins on treatment with ammonia in methanol. in antiviral studies the most active compounds against both hsv-1 and hsv-2 were 5-(2-thienylmethylene)-3-phenyl-2-(2,3,4,6- tetra-o-acetyl-beta-d-glucopyranosyl)-2-th ... | 1994 | 8289203 |
| herpes simplex type 2 in a patient with mollaret's meningitis: demonstration by polymerase chain reaction. | we describe a patient with recurrent self-limited aseptic meningitis meeting the criteria for mollaret's syndrome, in whom extensive serological investigation and cerebrospinal fluid analysis failed to reveal a specific cause. using polymerase chain reaction techniques to amplify herpes simplex viral dna, we were able to identify the presence of herpes simplex virus type 2 in the cerebrospinal fluid. the duration of clinical illness may have been shortened by treatment with intravenous acyclovir ... | 1994 | 8285581 |
| foscarnet-resistant herpes simplex virus infection in patients with aids. | six human immunodeficiency virus-infected patients had clinical lesions of herpes simplex virus (hsv) type 2 that showed in vitro resistance to foscarnet. in each patient, lesions were unresponsive to foscarnet therapy or developed during daily suppressive foscarnet. five patients had a history of intermittent or chronic foscarnet use for the management of acyclovir-resistant hsv infection, and 1 was receiving daily foscarnet for suppression of cytomegalovirus retinitis. seven of 10 foscarnet-re ... | 1994 | 8277181 |
| recombinant human fab to glycoprotein d neutralizes infectivity and prevents cell-to-cell transmission of herpes simplex viruses 1 and 2 in vitro. | herpes simplex viruses 1 and 2 (hsv-1 and -2) are associated with a number of conditions of varying severity, which are only partially responsive to current therapies. human antibodies to the viruses offer a potential alternative. we describe here the generation of panels of human monoclonal fab fragments to hsv-1 and -2 by panning a phage display combinatorial antibody library against whole lysates from the two viruses. each lysate selected a largely distinct set of fabs, although all of the fa ... | 1994 | 8278393 |
| tests for detecting herpes simplex virus and varicella-zoster virus infections. | several laboratory diagnostic methods are available for the diagnosis, differentiation, and subtyping of hsv and vzv infections. in the office or at the bedside of a hospitalized patient, a positive tzanck smear preparation is an inexpensive, rapid, and morphologic technique for confirming a suspected diagnosis of a herpesvirus infection. an expedient, slightly more expensive, reliable technique for establishing a hsv infection, yet not able to differentiate the subtype of that infection, is a r ... | 1994 | 8143385 |
| a population-based seroepidemiological study of cervical cancer. | the epidemiology of cervical cancer indicates the presence of a sexually transmitted risk factor, attributable at least in part to infection with human papillomavirus (hpv) type 16 or 18. we performed a seroepidemiological study of hpv and cervical cancer in the counties of västerbotten and norrbotten in northern sweden, a low-risk area for cervical cancer. sera from 94 cases of incident cervical cancer were matched against 188 age- and sex-matched controls derived from a population-based blood ... | 1994 | 8261434 |
| herpes simplex virus latency in tissue culture cells. | we have developed tissue culture systems which mimic certain aspects of herpes simplex virus (hsv) latency, in order to facilitate molecular studies of the phenomenon. the initial approach utilised incubation at 42 degrees c to suppress virus replication and establish stable latency after infection of human fibroblasts at low multiplicity with hsv-2. latent virus was reactivated efficiently by superinfection of cultures with hsv-1 or human cytomegalovirus. improvement of the system was achieved ... | 1994 | 8542396 |
| suspension culture of hela cells in protein-free medium: sensitivity to human pathogenic viruses. | human adherent hela-pf cells grown for 5 years in a protein-free 1:1 nutrient mixture of dulbecco's modified mem and ham's f12 (dmem-f12) were established in suspension culture. the cells grew in protein-free dmem-f12 (using magnetically stirred flasks) as a monodisperse suspension with a population doubling time of 28 h. the cells were infected with poliovirus types 2 and 3, herpes simplex virus types 1 and 2 (hsv-1 and hsv-2), respiratory syncytial virus (rsv), echovirus 6 and adenoviruses 3 a ... | 1994 | 8586536 |
| congenital infections caused by varicella zoster virus and herpes simplex virus. | congenital infections are caused by both varicella-zoster virus (vzv) and herpes simplex virus type 2 (hsv-2). the vzv fetopathy and the hsv fetopathy exhibit several similarities. both affect the skin, eyes and brain, often with devastating consequences. congenital vzv infection also may damage portions of the cervical or lumbosacral plexi, an insult that leads to denervation and maldevelopment of an extremity. as is evident from the pathology, most of the fetal sequelae caused by intrauterine ... | 1994 | 9422218 |
| hla class i molecules are not transported to the cell surface in cells infected with herpes simplex virus types 1 and 2. | to assess the effect of herpes simplex virus (hsv) on assembly and transport of class i mhc molecules, we compared class i mhc immunoprecipitated from metabolically labeled infected and uninfected human dermal fibroblasts. the immunoprecipitates were analyzed by isoelectric focusing, allowing identification of individual class i alleles and assessment of transport through the golgi apparatus by the sialation of carbohydrate residues. in cells infected with wild-type hsv, class i synthesis was re ... | 1994 | 8144880 |
| a mouse model for studies of mucosal immunity to vaginal infection by herpes simplex virus type 2. | the role of mucosal immunity in defense of the female genital tract against pathogens such as herpes simplex virus-2 (hsv-2) is poorly understood. here we explored the use of a new mouse model to determine whether local immune events in the vagina of immune animals may protect them against genital herpes. | 1994 | 8145530 |
| [ribonucleoside and 2'-deoxyribonucleoside 5'-phosphonates: synthesis and antiviral activity]. | groups of 5'-phosphonates of natural 2'-deoxynucleosides and ribonucleosides were synthesized by condensation of 3'-acetylated 2'-deoxynucleosides or 2',3'-substituted (2',3'-o-isopropylidene, 2',3'-o-methoxymethylene, or 2',3'-o-ethoxymethylene) ribonucleosides. as condensing agents, either n,n'-dicyclohexylcarbodiimide or 2,4,6-triisopropylbenzenesulphonyl chloride were used. nucleoside 5'-ethoxycarbonyl-phosphonates were converted into corresponding nucleoside 5'-aminocarbonylphosphonates by ... | 1994 | 8145753 |
| herpes simplex virus infections of women and their offspring: implications for a developed society. | herpes simplex virus infections of humans have been known since ancient times. contemporary society has witnessed a series of devastating manifestations of herpes simplex virus infections--namely, genital herpes simplex virus infection and neonatal herpes simplex virus infection. with the evolution of society, particularly advances in birth control and increasing promiscuity, the seroprevalence of herpes simplex virus type 2 infections has increased worldwide, however, more so in developed socie ... | 1994 | 8146137 |
| efficacy of a-73209, a potent orally active agent against vzv and hsv infections. | a-73209 is a novel oxetanocin derivative with potent in vitro and in vivo activity against vzv, hsv-1, and hsv-2. a-73209 was two logs more potent than acyclovir against five thymidine kinase positive (tk+) strains of vzv in vitro (mean ec50 0.01 vs. 1.22 micrograms/ml). the activity of a-73209 was one log more potent than acyclovir against tk+ hsv-1 strains in vitro (ec50 = 0.03 vs. 0.32 micrograms/ml). a-73209 yielded a mean ec50 of 2.2 micrograms/ml compared to a mean ec50 of 0.37 micrograms/ ... | 1994 | 8147583 |
| photodynamic inactivation of herpes viruses with phthalocyanine derivatives. | the antiviral photosensitization capacity of 11 different phthalocyanine (pc) derivatives was examined using herpes simplex virus-1, herpes simplex virus-2 and varicella zoster virus in the search for the most potent sensitizers for viral decontamination of blood. the kinetics of viral photoinactivation were resolved during the stages of viral adsorption and penetration into the host cells. the capacity of pc in the photodynamic inactivation of viruses was compared with that of merocyanine 540 ( ... | 1994 | 8151454 |
| identification of a dimerization domain in the c-terminal segment of the ie110 transactivator protein from herpes simplex virus. | the 775-amino-acid ie110 (or icp0) phosphoprotein of herpes simplex virus (hsv) functions as an accessory transcription factor during the lytic cycle and plays a critical role in reactivation from latent infection. by immunofluorescence analysis, ie110 localizes in a novel pattern consisting of several dozen spherical punctate granules in the nuclei of dna-transfected cells. we constructed a hybrid version of ie110 that contained an epitope-tagged domain from the n terminus of the hsv ie175 prot ... | 1994 | 8151788 |
| sexually transmitted diseases and other risk factors for cervical dysplasia among southwestern hispanic and non-hispanic white women. | to assess risk factors for high-grade cervical dysplasia among southwestern hispanic and non-hispanic white women. | 1994 | 8151876 |
| [herpesvirus pneumonia]. | 1994 | 8151919 | |
| passive immunization of the vagina protects mice against vaginal transmission of genital herpes infections. | vaginal application of human herpes simplex virus (hsv) antiserum, complement-inactivated antiserum, or igg purified from antiserum protected mice (p < .001, p < .001, and p < .01, respectively) from visible signs of genital hsv-2 infection after subsequent vaginal inoculation with hsv-2 (10 id50). vaginal application of an anti-hsv-2 monoclonal antibody (mab iii-174) also protected mice against infection. this mab, a neutralizing mouse igg2a against glycoprotein d, prevented infection as determ ... | 1994 | 8158042 |
| 8-hydroxymanzamine a, a beta-carboline alkaloid from a sponge, pachypellina sp. | the previously described manzamine a [1] and the new 8-hydroxymanzamine a [2] were isolated from an undescribed sponge, pachypellina sp. both compounds exhibit moderate antitumor and anti-hsv-ii activity. | 1994 | 8158160 |
| vaccinating guinea pigs with recombinant glycoprotein d of herpes simplex virus in an efficacious adjuvant formulation elicits protection against vaginal infection. | guinea pigs were immunized with glycoprotein gd-2t in saf-m or saline, then challenged with herpes simplex virus, type 2 (hsv-2). animals given gd-2t in saf-m had higher anti-gd-2t antibodies, fewer and less severe vaginal lesions, and decreased ganglionic latency compared to animals given gd-2t in saline. leucocytes from animals vaccinated with gd-2t in saf-m had greater proliferative responses to gd-2t in vitro than cells from control animals. mhc ii-restricted, gd-2t-specific cytotoxic t cell ... | 1994 | 8165851 |
| disposition of antigen-presenting liposomes in vivo: effect on presentation of herpes simplex virus antigen rgd. | antigen-presenting liposomes (apls) containing a lipophilic derivative of muramyl tripeptide (mtp-pe) have previously been shown to enhance the immunotherapeutic effects mediated by hsv recombinant protein gd (rgd) after hsv type 2 infection is established. in this study, both the in vivo disposition of rgd and the immunological activity of in vivo-delivered rgd were determined. following intravenous administration, most of the liposome-encapsulated rgd accumulated rapidly, mainly in the spleen, ... | 1994 | 8165856 |
| animal models of viral sexually transmitted diseases. | 1994 | 8166948 | |
| quantity of latency-associated transcript produced by herpes simplex virus is not predictive of the frequency of experimental recurrent genital herpes. | the role of the latency-associated transcript (lat) in control of recurrent herpes simplex virus type 2 (hsv-2) infection was investigated by examining whether lat concentration in vitro during productive infection or in ganglia during latency correlated with frequency of recurrent genital herpes. clinical hsv-2 isolates from frequent or infrequent recurrent genital disease produced comparable amounts of glycoprotein d and infected cell polypeptide 0 rna, but the isolate from frequent disease pr ... | 1994 | 8169396 |
| neonatal herpes simplex virus infection: pathogenesis and treatment in the guinea pig. | intranasal inoculation of newborn guinea pigs with herpes simplex virus (hsv) resulted in local skin-eye-mouth (sem), central nervous system (cns), and disseminated infections with high but not universal mortality. cutaneous hsv inoculation produced self-limited sem infection without evidence of cns or disseminated disease. hsv infection of the upper respiratory tract of the newborn guinea pig resulted in early spread to the cns and frequent viral dissemination. the outcome of infection was favo ... | 1994 | 8169425 |
| direct recovery of herpes simplex virus (hsv)-specific t lymphocyte clones from recurrent genital hsv-2 lesions. | t lymphocytes were recovered from recurrent vesicular and ulcerative herpes simplex virus type 2 (hsv-2) lesions from 4 patients and cloned without exogenous secondary antigenic stimulation. resultant cultures were screened for antigen-specific proliferative and cytotoxic responses. of the 39 hsv-specific clones recovered, all were cd3-positive; 38 were cd4-positive and 1 was cd8-positive. of the t cell clones recovered directly from lesions, 6%-10% had hsv-specific proliferative responses, in c ... | 1994 | 8169426 |
| prevalence and epidemiologic correlates of human t cell lymphotropic virus infection among intravenous drug users. | intravenous drug users (ivdus) in seattle (n = 213) were studied to identify the prevalence and predominant types of and risk factors for human t cell lymphotropic virus (htlv) infection. detailed questionnaires, serologic screening, and polymerase chain reaction analysis (for a subset) were used. evidence of htlv infection was found in 16.5%, of which 89% were htlv-ii. htlv infection was significantly associated with nonwhite race, older age, more years of intravenous drug use, prior use of her ... | 1994 | 8169427 |
| [biological properties of hemagglutinin of herpes simplex virus type 1]. | herpes simplex virus type 1 (hsv-1) has been recently known to agglutinate mouse erythrocytes. we performed the hemagglutination (ha) test under various conditions to investigate the nature of hemagglutinin of hsv-1. ha of mouse erythrocytes was observed with glycoprotein c (gc)-positive hsv-1 strains but not with gc-negative hsv-1 mutants and hsv-2 strains. a recombinant hsv-1 constructed by transfecting a gc-negative strain with a gc-1 gene regained full ha activity. the erythrocytes from seve ... | 1994 | 8175105 |
| in situ polymerase chain reaction: localization of hsv-2 dna sequences in infections of the nervous system. | to detect and localize a herpes simplex virus type 2 (hsv-2) thymidine kinase gene sequence in paraffin sections of brains and trigeminal ganglia of infected mice, an in situ polymerase chain reaction (ispcr) protocol was developed. using a single pair of primers, a 110 base pair dna target sequence, and incorporation of a digoxigenin-labelled nucleotide during amplification, this procedure permitted rapid, specific, reproducible detection of infected cells. during acute brain infection, cells l ... | 1994 | 8175948 |
| the transmembrane domain of the large subunit of hsv-2 ribonucleotide reductase (icp10) is required for protein kinase activity and transformation-related signaling pathways that result in ras activation. | the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10) is a chimera consisting of a ser/thr protein kinase (pk) with features of a transmembrane (tm) helical segment localized at the amino terminus, and the rr1 domain localized at the carboxy terminus. to elucidate the role of the tm segment in icp10-mediated transformation we established cell lines that constitutively express icp10 (jhla1) or its tm deleted mutant p139tm (jhl15). icp10 was associated with purified jhl ... | 1994 | 8178446 |
| the concept of immune-based therapies in chronic viral infections. | treatment of recurrent herpes simplex virus (hsv) infection with a vaccine was attempted as early as 1938. the concept of immune therapy is based on the premise that vaccine administration may augment host immunity, resulting in better control of the infection. the guinea pig model is useful in the study of recurrent hsv infection for several reasons. this animal model mimics human infection, with self-limited primary vulvovaginitis developing after inoculation with hsv. despite a full range of ... | 1994 | 8182508 |
| a rapid simple in situ hybridization method for herpes simplex virus employing a synthetic biotin-labeled oligonucleotide probe: a comparison with immunohistochemical methods for hsv detection. | we examined 28 paraffin-embedded tissue specimens with histologic evidence of herpes virus infection by in situ hybridization (ish) utilizing manual capillary action technology (microprobe staining system) and a 21 base synthetic multibiotinylated oligonucleotide probe from the hsv glycoprotein c region. the results were compared to a rapid simple immunohistochemical (ihc) protocol for detection of hsv proteins. hsv was detected by ish and ihc in all but one specimen which was shown to be positi ... | 1994 | 8189321 |
| cloning and expression of an equine herpesvirus 1 origin-binding protein. | equine herpesvirus 1 (ehv-1) is an important pathogen of horses and is closely related to several important human pathogens, herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) and varicella-zoster virus. the ehv-1 genome contains open reading frames similar in sequence to the hsv-1 replication genes. pcr was used to clone ehv-1 gene 53, which is similar in sequence to the hsv-1 ul9 gene. the gene 53 product has regions of striking similarity to the hsv-1 ul9 and vzv gene 51 products. in vitro ... | 1994 | 8189505 |
| neuron-specific restriction of a herpes simplex virus recombinant maps to the ul5 gene. | we have previously shown that, when compared with either parent, a herpes simplex virus type 1/herpes simplex virus type 2 intertypic recombinant (r13-1) is attenuated by 10,000-fold with respect to neurovirulence in mice. despite this, after intracranial inoculation, r13-1 replicated to titers of 10(5) pfu per brain. we present evidence that the restriction is specific for replication in neurons and have taken a three-step approach in determining the basis of the attenuation by (i) characterizi ... | 1994 | 8189514 |
| mannan-binding protein and bovine conglutinin mediate enhancement of herpes simplex virus type 2 infection in mice. | a broad range of plant lectins have recently been shown to inhibit the infectivity of herpes simplex virus type 1 (hsv-1) in vitro. we decided to investigate the role of mammalian lectins in infection with herpes simplex virus. two lectins, conglutinin and mannan-binding protein (also called mannose-binding protein, mbp), belonging to the collectin family of lectins, were examined. four week-old balb/c mice were injected subcutaneously with 100 micrograms bovine conglutinin or 50 micrograms huma ... | 1994 | 8191219 |
| a randomized, double-blind, comparative trial comparing high- and standard-dose oral acyclovir for first-episode genital herpes infections. | orally administered acyclovir ameliorates the clinical course and decreases the duration of viral shedding in patients with first-episode genital herpes infections. we investigated in a randomized, double-blind, comparative trial whether a higher (4 g) than standard (1 g) daily dose of oral acyclovir results in greater clinical benefit and influences the time to first recurrence. a total of 139 patients with first-episode genital herpes were randomized to receive orally 4 or 1 g of acyclovir dai ... | 1994 | 8192438 |
| autopsy findings in two cases of neonatal herpes simplex virus infection: detection of virus by immunohistochemistry, in situ hybridization and the polymerase chain reaction. | we describe the pathological findings in two fatal cases of neonatal infection with herpes simplex virus. one had an encephalitis caused by herpes simplex virus type 2 (hsv-2); the other had a disseminated infection with herpes simplex virus type 1 (hsv-1). confirmation of the diagnosis was obtained by use of the polymerase chain reaction to amplify viral dna from paraffin sections of autopsy tissues. by using primers which amplify fragments of the hsv-1 thymidine kinase gene and hsv-2 glycoprot ... | 1994 | 8200626 |
| beta-carbolines from the blue-green alga dichothrix baueriana. | three new chlorine-containing beta-carbolines, bauerines a-c (1-3), have been isolted from the terrestrial blue-green alga dichothrix baueriana go-25-2, and identifying mass and nmr spectral analysis. the alkaloids show activity against herpes simplex virus type 2. | 1994 | 8201316 |
| acyclovir-resistant neonatal herpes simplex virus infection of the larynx. | a 10-day-old infant with stridor was found to have herpes simplex virus type 2 infection of the larynx. the infant's poor clinical response to both acyclovir and foscarnet prompted extensive clinical and virologic evaluations, which revealed acyclovir-resistant herpes simplex virus. | 1994 | 8201488 |
| herpes simplex virus type 2 infection of heterosexual men attending a sexual health centre. | to identify risk factors, particularly circumcision status, associated with serological evidence of herpes simplex virus type 2 (hsv-2) infection of heterosexual men. | 1994 | 8202004 |
| human t cell lymphotropic virus type i infection among female sex workers in peru. | four hundred female sex workers attending a sexually transmitted disease clinic in lima, peru, were interviewed for demographic information and medical, contraceptive, and sexual practice histories. cervical cultures were done for neisseria gonorrhoeae and chlamydia trachomatis, and serum was tested for antibodies to human immunodeficiency virus, human t cell lymphotropic virus type i (htlv-i), treponema pallidum, c. trachomatis, herpes simplex virus type 2 (hsv-2), and haemophilus ducreyi. the ... | 1994 | 8133088 |
| immunotherapy of acute and recurrent herpes simplex virus type 2 infection with an adjuvant-free form of recombinant glycoprotein d-interleukin-2 fusion protein. | previous studies demonstrated that the adjuvant-free form of a fusion protein consisting of a truncated herpes simplex virus type 1 (hsv-1) glycoprotein d and human interleukin-2 (tgd-il-2) elicited superior protective immunity in mice. in this study, the immunotherapeutic efficacy of tgd-il-2 against vaginal hsv-2 infection was investigated using a guinea pig model. footpad injections of tgd-il-2 (12.5 micrograms/dose) after the onset of primary lesions strongly suppressed recurrence in the chr ... | 1994 | 8133093 |
| a rapid screen test for in vitro susceptibility of clinical herpes simplex virus isolates. | a rapid screening test for antiviral susceptibility to acyclovir and foscarnet was evaluated in 320 herpes simplex virus (hsv) isolates from 197 patients. of the isolates tested (16% hsv-1, 84% hsv-2), 60% were resistant in vitro to acyclovir and 5% were resistant to foscarnet using the plaque reduction assay. the rapid screening test, requiring only 3 days to yield a preliminary susceptibility result, was highly associated with the results of the plaque reduction assay for acyclovir and foscarn ... | 1994 | 8133105 |
| hsv-2 dna persistence in astrocytes of the trigeminal root entry zone: double labeling by in situ pcr and immunohistochemistry. | a previous study using an in situ polymerase chain reaction (pcr) amplification method showed persistent herpes simplex virus type 2 (hsv-2) dna sequences in brains of experimentally infected mice, particularly in cells of the pons near the trigeminal root entry zone. the present study was undertaken to identify the cns cell type(s) that persistently harbor hsv dna and to define the associated pathology. tissue sections including the trigeminal root were immunoreacted to detect cellular antigens ... | 1994 | 8120536 |
| encapsidation defectiveness of herpes simplex virus type 2 during replication at acid ph condition. | the maximal yield of herpes simplex virus type 2 (hsv-2) grown at ph 6.5 decreased 10(2)-10(3) fold compared to that recovered at ph 7.5. electron microscopic observation of the infected cells maintained at these 2 ph conditions indicated that approximately equal amounts of immature virions were synthesized 6 hours after infection. however, at 18 hours post infection the majority of viruses present in the nucleus of infected cells maintained at ph 6.5 were empty or partially cored capsids with s ... | 1994 | 7872993 |
| herpes simplex virus type 2 induced retinal necrosis in balb/c mice. | we injected herpes simplex virus type 2 of ms- or g-strain into the anterior chamber of balb/c mice. in the contralateral eye inflammatory cell infiltration began in the ciliary body; focal retinitis, detected by day 8, led to total destruction of the retina by day 10. contralateral disease was observed in 75% of mice inoculated with 8 x 10(3) pfu herpes simplex virus type 2, but in only 20% of mice receiving 80 pfu herpes simplex virus type 2. still this low concentration, however, produced a s ... | 1994 | 8079627 |
| biological response of recombinant interleukin-7 on herpes simplex virus infection in guinea-pigs. | we investigated the biological activity of human recombinant interleukin-7 (rhil-7) on guinea-pig cells in vitro and in vivo. rhil-7 can stimulate plastic non-adherent cells of guinea-pigs to proliferate in vitro at a degree comparable to that of human cells. administration of rhil-7 to guinea-pigs significantly increased their white blood cell concentration, specifically the lymphocyte population. the effect of rhil-7 on circulating blood cells was dose dependent, in that 14 doses of twice dail ... | 1994 | 8085384 |
| herpes simplex virus and oral mucositis in children with cancer. | the relationship between herpes simplex virus (hsv) and oral mucositis was investigated in children undergoing antineoplastic chemotherapy. hsv culture was performed in 20 children with stomatitis developing after antineoplastic chemotherapy. viral isolates were typed and susceptibility to acyclovir was investigated. the virus was isolated from oral lesions in 10 of 20 children with severe oral mucositis. viral reactivation was the most likely explanation in most cases, since hsv was isolated in ... | 1994 | 8087447 |
| sexually transmitted diseases in hiv-infected persons. | it appears incontestable that there is a link between genital ulcer disease and hiv infection. on the one hand the natural history and response to therapy of syphilis, hsv-2, and chancroid are all modified by the immunosuppressive effects of hiv infection. on the other hand, hiv transmission is probably facilitated by the disruption of the normal epithelial barriers of the genital organs caused by these ulcerative infections. information is somewhat less convincing that a similar association exi ... | 1994 | 8089470 |
| [the production of recombinant polypeptides from the herpes simplex viruses types 1 and 2]. | the genes ie175, ie63, ie68, ie12, ul29 and ul19 of herpes simplex virus types 1 and 2 were cloned. fragments of these genes were cloned into open reading frame expression vector pex1-3. recombinant proteins containing amino acid sequences of icp4, icp27, icp22, icp47, major dna-binding protein and major capsid protein were expressed in e. coli cells by fusion with cro-beta-galactosidase proteins. | 1994 | 8091748 |
| chemiluminescent and colorimetric detection of a fluorescein-labelled probe and a digoxigenin-labelled probe after a single hybridization step. | the objective of the simple and fast method we describe is the simultaneous hybridization of two non-radioactive probes and their detection from the same blot, using two different systems. these two probes are synthesized by pcr: one is labelled with fluorescein and the other with digoxigenin. the former is detected by chemiluminescence and the latter by colorimetry. we applied this rapid and simple method to the specific detection of multiplex polymerase chain reaction products. we used the hum ... | 1994 | 7877636 |
| placebo-controlled trial of vaccination with recombinant glycoprotein d of herpes simplex virus type 2 for immunotherapy of genital herpes. | immunotherapy of chronic viral diseases with vaccines is an important but unproven concept. we investigated the effect of a vaccine containing recombinant glycoprotein d (gd2) of herpes simplex virus type 2 (hsv-2) on the frequency of symptomatic outbreaks in patients with genital herpes. 98 patients with documented genital herpes who reported 4-14 recurrences per year were enrolled in a double-blind, placebo-controlled trial. subjects received injections of either 100 micrograms gd2 in alum or ... | 1994 | 7911177 |
| purification of the integral membrane glycoproteins d of herpes simplex virus types 1 and 2, produced in the recombinant baculovirus expression system, by ion-exchange high-performance liquid chromatography. | selective elution of sendai virus integral membrane proteins by ion-exchange high-performance liquid chromatography (hpiec) using different detergent concentrations was reported before [s. welling-wester, m. freijlbrief, d.g.a.m. koedijk, m.a. braaksma, b.r.k. douma and g.w. welling, j. chromatogr., 646 (1993) 37]. in the present study this novel approach was applied to the purification of the integral membrane glycoprotein d of herpes simplex virus type 1 and 2. the glycoproteins d of types 1 ( ... | 1994 | 7921180 |
| [epidemiological study on risk factors of cervical cancer]. | the relationship between cervical cancer and risk factors including virus infection of cervix was studied with methods of molecular and serological epidemiology. dna extracted from cervical biopsies taken from 57 cases and 51 controls were tested for hpv6/11, hpv16/18 by dot-blot hybridization and for hsv-2 by pcr. sera were detected for hcmv-igm by elisa. the results and risk factors were analyzed by logistic regression model. the multiple logistic regression model results showed that hpv16/18 ... | 1994 | 7923330 |
| [chronic fatigue and herpes virus antibodies]. | 1994 | 7924923 | |
| activity of two synthetic amphiphilic peptides and magainin-2 against herpes simplex virus types 1 and 2. | the in vitro antiviral activity of two amphiphilic synthetic peptides, modelin-1 (mod-1) and modelin-5 (mod-5), and of the natural antibacterial peptide magainin-2 (mag-2) against herpes simplex viruses type 1 (hsv-1) and 2 (hsv-2) were evaluated. the peptides were incubated with the virus, i.e. direct inactivation, and their effects examined by means of plaque reduction assay and/or reduction in virus yield. only mod-1 displayed a strong antiviral effect against hsv-1 and hsv-2, with 50% effect ... | 1994 | 7928088 |
| a thymidine kinase deficient hsv-2 strain causes acute keratitis and establishes trigeminal ganglionic latency, but poorly reactivates in vivo. | the incidence of herpetic keratitis following intranasal or direct ocular infection with thymidine kinase-negative (tk-) strains of herpes simplex virus (hsv)-2 has not been well studied, and the role of the tk gene in the establishment of latency and virus reactivation is controversial. to determine whether a tk- strain of hsv-2 could establish trigeminal ganglionic latency and be reactivated in vivo to produce recurrent keratitis or nervous system infection, an animal model of acute and recurr ... | 1994 | 7931192 |
| herpes simplex virus-induced expression of 70 kda heat shock protein (hsp70) requires early protein synthesis but not viral dna replication. | the major 70 kda heat shock protein (hsp70), which is scarcely expressed in unstressed rodent cells, was apparently induced by infection with herpes simplex virus (hsv). infection with hsv types 1 and 2 elevated hsp70 mrna levels within 4 hr post-infection. hsp70 synthesis and accumulation increased in hsv-infected cells. irradiation of hsv with uv-light abolished the ability to induce hsp70 mrna. inhibitors of viral dna synthesis did not affect the induction of hsp70 in infected cells. protein ... | 1994 | 7935054 |
| a vaccinia virus--herpes simplex virus (hsv) glycoprotein b1 recombinant or an hsv vaccine overcome the hsv type 2 induced humoral immunosuppression and protect against vaginal challenge in balb/c mice. | primary infections with herpes simplex virus type 2 (hsv-2) suppress the antibody response to secondary hsv-1 and -2 infections in the balb/c mouse. in contrast, a challenge by the i.p. route using a vaccinia virus-hsv-1 glycoprotein b (vv gb1) recombinant induces a significant enhancement of the antibody response. this booster reaction is also observed if a challenge with a formalin-inactivated hsv-1 vaccine is performed. although no or low humoral and vaginal antibodies are detectable after a ... | 1994 | 7935163 |
| herpes simplex myelitis as a cause of acute necrotizing myelitis syndrome. | we report a patient with herpes simplex virus (hsv) myelitis with a clinical history of rapidly progressive spinal cord dysfunction and extensive necrosis of the lumbosacral spinal cord at autopsy. the etiologic agent was hsv type 2. the close resemblance of the clinical features of this case to the syndrome of acute necrotizing myelitis emphasizes the need to recognize hsv myelitis as a cause of this syndrome, since it is diagnosable during life by culturing the csf and is treatable. | 1994 | 7936255 |
| demonstration of herpes simplex type 2 in the cerebrospinal fluid of two patients with recurrent lymphocytic meningitis. | to investigate the aetiology of recurrent lymphocytic meningitis in two patients with a past history of recurrent genital herpes simplex infection. | 1994 | 7936462 |
| transinhibition of herpes simplex virus replication by an inducible cell-resident gene encoding a dysfunctional vp19c capsid protein. | this study demonstrates that cells expressing a dysfunctional analog of a herpes simplex virus (hsv) capsid protein inhibits hsv replication. vero cell lines expressing hsv-1 capsid protein vp19c/beta-galactosidase fusion proteins were constructed and tested for their kinetics of expression, intracellular location, and ability to interfere with hsv replication. two chimeric genes were constructed for these studies. the larger chimeric gene encodes the amino terminal 327 amino acids (aa) of vp19c ... | 1994 | 7941701 |
| rapid diagnosis of cutaneous herpes simplex infections using specific monoclonal antibodies. | one hundred children with suspected herpes simplex virus (hsv) infection and 20 controls were studied to compare a rapid immunofluorescence (rif) test for detection and typing of hsv from smears of lesions with standard viral culture. the rif test was evaluated for ease of use and speed of diagnosis. rif and/or culture were positive in 64% of patients. all infections diagnosed by rif and culture were hsv type 1. in 92% of patients rif and culture results were in concordance. in 57 cases, rif and ... | 1994 | 7955468 |
| determinants of human papillomavirus types 16 and 18 infections in the lower female genital tract in an italian population. | the prevalences of human papillomavirus (hpv) types 16 and 18 infection were determined by a non isotopic molecular hybridization assay on cervical scrapes from 738 women affected by gynaecological lesions different from malignancies. the correlation with known epidemiological risk factors for cervical neoplasia, such as sexual habits, smoking and pill use, was investigated. the overall hpv prevalence rate was 29.8% (220/738). viral dna sequences were detected in 26.9% (122/452) of morphological ... | 1994 | 7957325 |
| standardisation of a microplate in situ elisa (mise-test) for the susceptibility testing of herpes simplex virus to acyclovir. | viral susceptibility testing has been traditionally performed by plaque reduction assay (pra) which is labour intensive, time consuming and requires subjective input by the reader. an in situ enzyme-linked immunosorbent assay (elisa) method has been developed with the potential to overcome many of the limitations of pra, and has been applied to a variety of viruses. previous reports of elisa susceptibility assays have shown little standardisation between these methods, or any significant analysi ... | 1994 | 7962265 |
| alteration of intracerebral cytokine production in mice infected with herpes simplex virus types 1 and 2. | previously we reported that a lethal strain of herpes simplex virus type 2 (hsv-2) infects the brain following ocular inoculation of mice. we now demonstrate that hsv-2 mediates an unusual intracellular sequestering of class ii major histocompatibility complex (mhc) antigens. with use of an rnase protection assay, we observed a selective inhibition of ifn-gamma and il-6 gene transcription in brains of mice infected with hsv-2. it is likely that the inhibition of cytokine gene expression was medi ... | 1994 | 7962482 |
| protective vaccination against primary and recurrent disease caused by herpes simplex virus (hsv) type 2 using a genetically disabled hsv-1. | the vaccine potential of a mutant herpes simplex virus (hsv) type 1, with a deletion in the glycoprotein h (gh) gene, was evaluated. the virus requires a gh-expressing cell line for multi-cycle growth but can complete a single cycle of infection in noncomplementing cells. such viruses, termed disc (disabled infectious single cycle) viruses, should be safe, yet still able to stimulate humoral and cell-mediated responses against a broad range of virus antigens in vaccinated hosts. prophylactic vac ... | 1994 | 7963701 |
| the influence of adjuvant on the therapeutic efficacy of a recombinant genital herpes vaccine. | the potential use of vaccines for treatment of chronic or persistent virus infections is an area of great interest and controversy. previous experiments have shown that the incidence and severity of spontaneous recurrent genital herpes in latently infected guinea pigs could be significantly reduced by vaccination with herpes simplex virus glycoprotein subunit vaccines. the current study shows the critical role of adjuvant in an effective formulation. immunization of previously infected guinea pi ... | 1994 | 7963702 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 45-1994. a 47-year-old man with inguinal lymphadenopathy and fever during preparation for a bone marrow transplant. | 1994 | 7969363 | |
| isolation of herpes simplex virus from sexually transmitted disease patients in ibadan, nigeria. | despite the public health importance of herpes simplex virus infection in nigeria, attempts have not been made to isolate and identify the virus. | 1994 | 7974067 |
| hsv-2 increases the mitochondrial aspartate amino transaminase characteristic of tumor cells. | a set of polypeptides has previously been described as being immunoprecipitated from tumor cell lysates by the sera of tumor-bearing animals (tbs) or by antisera raised to herpes simplex virus type 2 (hsv-2)-infected cells. these polypeptides were not immunoprecipitated from control cells. the principal polypeptides detected of mw 200, 90 (u90 and l90), 40, and 32 kda were increased by hsv-2 infection. this communication describes the purification of the 40-kda protein and its identification by ... | 1994 | 7975243 |