Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| genetics of natural resistance to herpes simplex virus type 1 latent infection of the peripheral nervous system in mice. | the genetics of natural resistance to the development of latent infection in the trigeminal ganglia of mice inoculated in the lip with herpes simplex virus type 1 (hsv-1) was examined. based on coefficients of a logistic regression relating latency to strain and hsv-1 concentration, inbred strains of mice formed a continuum of resistance ranging from most resistant (c57bl/6j) to most susceptible (pl/j). when these results were analysed along with latency data derived from studies employing a non ... | 1986 | 3007658 |
| characterization of a herpes simplex virus type 2-specified glycoprotein with affinity for n-acetylgalactosamine-specific lectins and its identification as g92k or gg. | extracts from herpes simplex virus type 2 (hsv-2)-infected cells were subjected to affinity chromatography with gel-bound helix pomatia lectin (hpa). only one hsv-2-specified glycoprotein was isolated by this procedure and the glycoprotein had an apparent molecular weight of 130 000 (130k). the hpa-binding glycoprotein was genetically mapped, using hsv-1 x hsv-2 intertypic recombinants into the short component of the hsv-2 genome. the mapping position, electrophoretic mobility and the antigenic ... | 1986 | 3007660 |
| novel herpes simplex virus type 1 glycoproteins identified by antiserum against a synthetic oligopeptide from the predicted product of gene us4. | gene us4 of herpes simplex virus type 1 (hsv-1) has been predicted, from dna sequence analysis, to encode a protein of molecular weight 25237 and its properties suggest it to be a membrane-associated protein. we have investigated this protein by raising antiserum to a synthetic oligopeptide corresponding to a stretch of amino acids from an internal hydrophilic region of the predicted sequence. this antiserum immunoprecipitates three glycoprotein species of apparent mol. wt. 37 000, 48 000 and 56 ... | 1986 | 3007661 |
| evidence that the 'active centre' of the herpes simplex virus thymidine kinase involves an interaction between three distinct regions of the polypeptide. | the nucleotide sequence of the coding region of the thymidine kinase gene from each of three mutant strains of herpes simplex virus type 1 and from the parental strain, sc16, has been determined. the mutants were known to express thymidine kinase enzymes with distinct substrate binding properties. consideration of the lesions in the genes responsible for these altered biochemical properties. consideration of the lesions in the genes responsible for these altered biochemical properties has led us ... | 1986 | 3007662 |
| synthesis, structure, and antitumor and antiviral activities of a series of 5-halouridine cyclic 3',5'-monophosphates. | a series of potential prodrug 5-halouridine 3',5'-cyclic monophosphates (5-x-cumps, x = f, cl, br, i, 1-4) has been prepared and tested for antitumor activity against murine leukemia l1210/0 and human lymphoblast raji/0 cells and their deoxythymidine kinase deficient (tk-) counterparts, as well as for antiviral activity in primary rabbit kidney cells infected with herpes simplex virus type 1 or 2, vaccinia virus, or vesicular stomatitis virus. the 5-halopyrimidine bases, nucleosides (5-x-u), and ... | 1986 | 3007759 |
| genetic determinants of growth phase-dependent and adenovirus 5-responsive expression of the chinese hamster thymidine kinase gene are contained within thymidine kinase mrna sequences. | we have constructed a chimeric thymidine kinase (tk) minigene, phe delta 6ha, which combines the complete coding and 3' noncoding regions of a chinese hamster tk cdna with the promoter region and 5' untranslated region of the tk gene of herpes simplex virus type 1. we have transformed rat 4 cells to tk+ with this gene and analyzed the pattern of tk gene expression in these transformants under various conditions of in vitro cell culture. we find that tk gene expression in these tk+ transformants ... | 1986 | 3023925 |
| the control of herpes simplex virus type-1 late gene transcription: a 'tata-box'/cap site region is sufficient for fully efficient regulated activity. | the transcriptional programme of herpes simplex virus type 1 (hsv-1) is organised into three principle phases; immediate-early (ie), early (e) and late. the appearance of ie gene products provides the switch for e transcription. abundant expression of late genes requires viral dna replication. there is some overlap between e and late genes according to their degree of dependence on dna replication. the pattern of expression of gene us11 is regulated with 'true-late' kinetics (johnson et al., 198 ... | 1986 | 3024102 |
| the dna replication origins of herpes simplex virus type 1 strain angelotti. | the nucleotide sequences of the origins of dna replication (ori) of the s- and l-component (oris, oril) of the herpes simplex virus type 1 (hsv-1) standard genome were determined from hsv-1 strain angelotti (ang). in contrast to other hsv-1 strains, the ang oris sequence revealed an insertion of an ta-dinucleotide in an otherwise very conserved but imperfect palindromic sequence of 47 bp. the oril sequence of the standard ang genome was found to be identical to that of an ang class ii defective ... | 1986 | 3024114 |
| a method for identifying the viral genes required for herpesvirus dna replication. | several laboratories have shown that transfected plasmid dnas containing either of the two known origins of herpes simplex virus (hsv) dna replication, oris or oril, are replicated in hsv-1-infected cells or in cells cotransfected with virion dna. i have found that hsv-1 (kos) dna digested to completion with the restriction enzyme xba i is as efficient as intact viral dna in supporting the in vivo replication of cotransfected plasmids containing oris. on the basis of this result, several of the ... | 1986 | 3024166 |
| viral antibodies in serum and cryoprecipitate of patients with essential mixed and secondary cryoglobulinemia. preliminary results. | antibodies to measles virus, cmv, hsv1, hsv2 and ebv have been tested in 9 patients with essential mixed cryoglobulinemia (emc) and 24 patients with cryoglobulinemia associated with lymphoproliferative, autoimmune or hepatic diseases (secondary cryoglobulinemia, sc). the assays were performed in serum, cryoprecipitate and supernatant by using immunoenzymatic methods. the highest prevalence was observed in the supernatant of both emc and sc patients. antibodies to measles virus show a serum/cryop ... | 1986 | 3024297 |
| a prospective study of unexplained nausea and vomiting after marrow transplantation. | we prospectively studied patients with enigmatic nausea and vomiting after allogeneic marrow transplantation to define the causes of this syndrome. fifty consecutive episodes of persistent vomiting were investigated using physical examination and laboratory tests, endoscopic biopsies and brushings, and clinical follow-up for four weeks. potential causes of vomiting were identified in 39 of the 50 cases (78%). fifteen cases had gastrointestinal infections (mainly herpesviruses), 13 had unsuspecte ... | 1986 | 3024371 |
| the nucleotide sequence of the gb glycoprotein gene of hsv-2 and comparison with the corresponding gene of hsv-1. | the nucleotide sequence of the gb glycoprotein gene of hsv-2 has been determined and compared with the homologous gene of hsv-1. the two genes are specified by the same total number of codons (904); eight additional codons of the hsv-1 gene are found within the signal sequence, and eight additional codons of the hsv-2 gene are found at three different sites in the gene. the signal cleavage, membrane-spanning, and eight potential n-linked oligosaccharide sites, as well as 5'- and 3'-regulatory si ... | 1986 | 3024391 |
| false positive indirect fluorescent antibody (ifa) tests for antibody to herpes simplex virus 1. comparison of four commercially available methods. | four commercially available herpes simplex virus 1 (hsv-1) indirect fluorescent antibody (ifa) kits were compared with the use of sera selected because they were negative for hsv antibody by complement fixation (cf less than 1:8) and by elisa (less than 1:100). however, 14 of 24 (58.3%) of these hsv-1 antibody-negative sera were positive at greater than or equal to 1:10 with the use of the hsv-1 ifa kit from electronucleonics, 15 of 24 (62.5%) were positive with the clinical sciences hsv-1 ifa k ... | 1986 | 3024480 |
| comparative methods for detection of thymidine kinase-deficient herpes simplex virus type 1 strains. | four methods for analyzing viral susceptibility to antiviral substances were compared. in two methods viral products were measured: late viral proteins were measured by an enzyme-linked immunosorbent assay and viral dna was measured by dna hybridization. infectious virus was quantified in the other two assays as the number of plaques and the yield of virus. the enzyme-linked immunosorbent assay procedure in our hands detected the smallest amounts (lowest proportions) of thymidine kinase-deficien ... | 1986 | 3024561 |
| mode of action, toxicity, pharmacokinetics, and efficacy of some new antiherpesvirus guanosine analogs related to buciclovir. | 9-[4-hydroxy-3-(hydroxymethyl)butyl]guanine (3hm-hbg), (rs)-9-[4-hydroxy-2-(hydroxymethyl)butyl]guanine ([+/-]2hm-hbg), and cis-9-(4-hydroxy-2-butenyl)guanine (2en-hbg), new acyclic guanosine analogs structurally related to buciclovir (bcv [(r)-9-(3,4-dihydroxybutyl)guanine]), were evaluated in parallel with buciclovir as anti-herpes simplex virus (hsv) agents. in cell cultures, replication of different strains of hsv type 1 (hsv-1) and hsv-2 was inhibited at nontoxic drug concentrations. the co ... | 1986 | 3024562 |
| [molecular genetics of the herpes simplex viruses: physical and functional mapping of a new syncytial locus in the region coding thymidine kinase and glycoprotein h]. | 1986 | 3024680 | |
| [molecular genetics of herpes simplex viruses: mapping of the mutation conferring resistance to benzhydrazone and of three syncytial loci simultaneously present in a mutant of the herpes simplex virus type 1]. | 1986 | 3024681 | |
| anti-herpes virus combinations in relation to drug resistance. | two broad categories of combinations have been subject to experimental investigation against hsv-1 and hsv-2; those consisting of an active antiviral drug and an inhibitor of its catabolism and those which contain two active antiviral agents. the latter seek to exploit biochemical mechanisms likely to lead to a synergistic or complementary interaction. examples of both types of combination for the suppression of resistance development and for the treatment of infection caused by resistant strain ... | 1986 | 3025155 |
| the antiviral activity against herpes simplex virus of the triterpenoid compounds carbenoxolone sodium and cicloxolone sodium. | dose-response experiments show that the presence of 300 microm cicloxolone sodium (ccx) or 500 microm carbenoxolone sodium (cbx) during the hsv replication cycle reduced the infectious virus yield by 10,000- to 100,000-fold: ccx is the more potent anti-herpes agent. hsv-2 replication was consistently more severely restricted by either drug than was that of hsv-1. the ed50 values obtained for either drug against hsv-1 or hsv-2 correlate well with data from dose-response curves. ccx, and to a less ... | 1986 | 3025156 |
| immune response to herpes simplex virus in patients with recurrent herpes labialis. ii. relationship between interferon production and cytotoxic responses. | the relationship between the development of cytotoxic cellular immune response to herpes simplex virus type i (hsv-1)-infected autologous cells and the production of interferon (ifn) was studied using in vitro secondary sensitization of peripheral blood leukocytes in subjects with recurrent herpes labialis (rhl) and in normal controls without any history of recurrent herpes labialis. there was a significant discordance between optimal hsv-1 antigen dose required for induction of peak cytotoxic r ... | 1986 | 3018660 |
| association of the herpes simplex virus regulatory protein icp4 with specific nucleotide sequences in dna. | we report that the herpes simplex virus (hsv) transcription regulatory protein designated icp4 is a component of a stable complex between protein and specific nucleotide sequences in double-stranded dna formed by addition of exogenous dna to either a crude extract obtained from hsv-1 infected cells or a partially purified preparation of native icp4. dna sites which are bound directly or indirectly to icp4 have been designated icp4/protein binding sites. three independent icp4/protein binding sit ... | 1986 | 3018669 |
| a dna binding protein specific for an origin of replication of herpes simplex virus type 1. | we have identified a protein that binds specifically to an origin of replication (oris) of the herpes simplex virus type 1 genome. the oris binding protein, detectable only in nuclear extracts of infected cells, shows the same time course of appearance as the herpesvirus-induced dna polymerase and the dna binding protein icp8. the partially purified oris binding protein generates a dnase i "footprint" that spans 18- of the 90-base-pair minimal oris sequence. the oris binding protein may, therefo ... | 1986 | 3018724 |
| antiherpetic effects of a human alpha interferon analog, ifn-alpha con1, in hamsters. | the efficacy of a novel consensus form of human alpha interferon designated ifn-alpha con1 was evaluated against herpesvirus infections in vitro and in vivo. at comparable antiviral concentrations, natural lymphoblastoid ifn, ifn-alpha con1, the molecular subtype ifn-alpha, and the hybrid ifn-alpha ad(bgl) obtained by recombinant dna methods conferred similar protection against herpes simplex virus type 1 and type 2 (hsv-2) infections of human cells in vitro. whereas 7 x 10(5) u of ifn-alpha ad( ... | 1986 | 3019238 |
| herpes simplex virus type 1 persistence and latency in cultured rabbit corneal epithelial cells, keratocytes, and endothelial cells. | cell cultures of rabbit corneal epithelium, keratocytes, and endothelium were used to determine the lytic cycle of herpes simplex virus type 1. viral growth was fastest in epithelial cells. a novel hsv-1 in-vitro latency system was established in the three distinct cell types. cell cultures were inoculated at low multiplicities of infection with hsv-1. temperature manipulation alone was used to induce and reactivate latent hsv-1 infections. the presence of cellular stress proteins was demonstrat ... | 1986 | 3019382 |
| five-year survival of patients with oral cancer and its association with antibody to herpes simplex virus. | levels of antibody to herpes simplex virus type 1 (hsv-1) were measured in 70 patients with untreated squamous cell carcinoma of the mouth. after treatment the actuarial survival was determined at quarterly intervals for 5 years and was found to be associated with the pretreatment level of antibody to the virus. patients with levels of igm antibody to hsv-1 which were above the median level had a 5-year survival of only 56% whereas those with levels below the median had a higher survival of 72%. ... | 1986 | 3019504 |
| specific transfer factor protects mice against lethal challenge with herpes simplex virus. | bovine transfer factor (tfd) specific to herpes simplex virus (hsv)1 or to hsv2 was prepared by immunizing calves with the corresponding virus. the tfd preparations were then injected into swiss mice in an attempt to protect them against a subsequent lethal challenge with hsv1 or hsv2 virus. it was thus shown that injection of anti-hsv tfd protects the mice against the corresponding hsv virus, whereas the injection of a nonspecific tfd (anti-cmv) fails to protect against a challenge with hsv1. f ... | 1986 | 3019568 |
| identification of an epstein-barr virus-coded thymidine kinase. | we have demonstrated the presence of an epstein-barr virus (ebv)-coded thymidine kinase (tk) by producing biochemically transformed, tk-positive mammalian cell lines using either microinjection of whole ebv virions or calcium phosphate-mediated transfection of the sali-b restriction endonuclease fragment of ebv dna. analysis of these cell lines showed that: (i) ebv dna was present in the cell lines, (ii) sequences from the sali-b restriction endonuclease fragment of ebv were expressed, (iii) a t ... | 1986 | 3019675 |
| hsv-1 recovery from ocular tissues after viral inoculation into the superior cervical ganglion. | new zealand albino rabbits were inoculated in the right superior cervical ganglion with 25 microliter of herpes simplex virus type 1 (hsv-1) (mckrae strain; 10(3) or 10(5) pfu/ml). positive tear film swabs were detected at least once in 28/32 (88%) of ipsilateral eyes and 6/32 (19%) of contralateral eyes beginning on postinoculation (pi) day 2-6. the average hsv-1 titer in the tear film was 4.0 x 10(3) pfu in ipsilateral eyes and 2.7 x 10(3) pfu in contralateral eyes, determined from eye washes ... | 1986 | 3019918 |
| a rapid and automated colorimetric assay for evaluating the sensitivity of herpes simplex strains to antiviral drugs. | a rapid and sensitive colorimetric assay has been developed to evaluate the sensitivity of herpes simplex viruses (hsv) to antiviral agents. the chessboard titration of viruses and antiviral drugs and the automatic reading and analysis of the data allows objective and accurate results to be rapidly obtained. virus sensitivity was expressed as an ed50 value which was the concentration of drug (micrograms/ml) reducing viral cpe by 50%. the ed50 values of antiviral drugs [acetylguanosine (acv), ido ... | 1986 | 3020058 |
| intranuclear localization of herpes simplex virus immediate-early and delayed-early proteins: evidence that icp 4 is associated with progeny virus dna. | the localization of icp 4, icp 8, dna polymerase and alkaline exonuclease within herpes simplex virus type 1 (hsv)-infected cells has been examined by immunofluorescence using specific antibodies to these proteins. cells were simultaneously counterstained with the dna-binding fluorochrome 4,6-diamidino-2-phenylindole (dapi) to reveal the intranuclear distribution of dna. these studies showed that in the absence of virus dna replication icp 4, icp 8 and dna polymerase were diffusely distributed t ... | 1986 | 3020158 |
| chromosome damage induced by herpes simplex virus type 1 in early infection. | the chromosome damage induced by herpes simplex virus type 1 (hsv-1) in vitro was examined up to 6 h after infection using ht-1080 cells. initial damage occurring within 3 h was specific, involving uncoiling of chromosome 1q12-21 and to a lesser extent the pericentric regions of chromosomes 9, 16 and satellited chromosomes. for the initial unwinding, synthesis of the immediate early class of hsv proteins needed to occur as was demonstrated using hsv-1 temperature-sensitive mutants tsk and tsb7 a ... | 1986 | 3020163 |
| dna sequence of the major capsid protein gene of herpes simplex virus type 1. | the dna sequence of the region of the herpes simplex virus type 1 genome encoding the major capsid protein was determined. the predicted protein contains 1374 amino acid residues and has a molecular weight of 149,075. comparisons of the amino acid sequence of this protein with those predicted from the published dna sequences of two other herpesviruses, varicella-zoster virus and epstein-barr virus, resulted in the identification of the major capsid protein gene in each genome. | 1986 | 3020164 |
| potential treatment of herpes simplex virus encephalitis by brain-specific delivery of trifluorothymidine using a dihydropyridine in equilibrium pyridinium salt type redox delivery system. | a newly described, drug-carrier delivery system in which a lipophilic derivative is enzymatically converted to a hydrophilic compound was used to treat experimental herpes simplex virus (hsv) encephalitis. because trifluorothymidine (tft) does not cross the blood brain barrier, the lipophilic dihydropyridine derivative 3'-(n-methyl-1, 4-dihydronicotinoyl)-5-'pivaloyltrifluorothymidine (dhtft) was synthesized and characterized by hplc. after intravenous administration of 20 mg/kg of dhtft to rats ... | 1986 | 3020165 |
| natural killer cell activity and head and neck cancer: a clinical assessment. | in 127 patients with squamous cell carcinoma of the upper aerodigestive system, an assessment of natural killer (nk) cell function was performed. the mean lytic unit (lu) value of this cancer population was noted to be less than the mean value of 67 age-matched controls assessed concurrently. the major determinant of cytolytic function was related to the growth pattern of the tumor. increased nk cell function was observed in patients with lesions that were more locally or regionally aggressive, ... | 1986 | 3020297 |
| localization and comparative nucleotide sequence analysis of the transforming domain in herpes simplex virus dna containing repetitive genetic elements. | the 7.5-kilobase bamhi e fragment (bamhi-e) of herpes simplex virus type 2 (hsv-2) dna (map position 0.533-0.583) encodes the 144-kda subunit of ribonucleotide reductase and induces the neoplastic transformation of immortalized cell lines. to define the minimal transforming region of bamhi-e, a series of subclones were constructed that spanned the entire fragment. these subclones were assayed for focus formation in rat-2 cells. removal of the promoter region from the viral 144-kda-protein gene l ... | 1986 | 3020562 |
| lack of evidence for intertypic recombinants in the pathogenesis of recurrent genital infections with herpes simplex virus type 1. | clinical observations indicate that herpes simplex virus type 1 (hsv-1) is significantly less likely than herpes simplex virus type 2 (hsv-2) to establish latency in (or reactivate from) sacral ganglionic tissue. in an effort to identify viral functions associated with latency, we analyzed hsv-1 isolates from three patients with established recurrent genital herpes and sought evidence of dna sequences and proteins similar to those found in hsv-2. by restriction endonuclease cleavage patterns and ... | 1986 | 3020720 |
| comparative studies on experimental latent herpesvirus infections. | two different models of virus persistence in laboratory animals, that of herpes simplex virus (hsv-1) and that of murine herpesvirus (mhv), are described. while hsv-1 is harboured in a nonproductive form in the regional sensory ganglion of rabbits and mice, the persistence of mhv in mouse lungs and spleen is of a productive (dynamic) type. since cultivation of kidney and trigeminal ganglion fragments enhanced the mhv recovery rate, one may assume that mhv persisted in a static form too, at least ... | 1986 | 3020779 |
| a simple repetitive sequence common to herpes simplex virus type 1 and human ribosomal dnas. | the simple sequence ggc was tandemly repeated in herpes simplex virus type 1 dna and human 28s rdna and its mature 28 s rrna transcript. the sequence homology was responsible for the observed hybridization between the two dnas under high stringency blot hybridization conditions. | 1986 | 3020785 |
| characterization and immunogenicity of hsv-1 antigens obtained following zwitterionic detergent treatment. | a preparation was obtained from herpesvirus hominis type 1 (hsv-1) infected cells using the zwitterionic detergent, empigen bb. the preparation was partially purified by ultracentrifugation over a cushion of 20% sucrose. serological characterization by elisa and immuno double diffusion, using both polyclonal and monoclonal antibodies shows this preparation to contain hsv glycoproteins, including gc, gd and ge. immunization of balb/c mice elicited serum antibody responses against both hsv-1 and h ... | 1986 | 3020821 |
| passage of herpes simplex virus type 1 on chick embryo fibroblasts confers virulence for chick embryos. | the pathogenesis of chorioallantoic membrane (cam) infection with herpes simplex virus 1 and 2 (hsv-1 and hsv-2) as well as chick embryo fibroblast (cef) passaged hsv-1 was studied. it was found that hsv-2 is at least a million-fold more virulent than hsv-1 as measured by pfu/ld50 ratios for the embryo. serial passage of hsv-1 in vitro on cef cells selected for a virus (cefp10) which, unlike its parental hsv-1 strain, is able to kill the embryo. the restriction endonuclease maps of cefp10 and it ... | 1986 | 3020830 |
| in vitro activity of ketoconazole against herpes simplex virus. | the effects of ketoconazole alone and in combination with acyclovir and adenine arabinoside upon the replication of herpes simplex virus types 1 and 2 (hsv-1 and -2) were investigated by using a yield reduction assay. ketoconazole demonstrated antiviral activity against hsv-1 and -2 and synergistic antiviral activity when it was combined with acyclovir. combinations of ketoconazole with adenine arabinoside resulted in either interference or indifference. the effects of ketoconazole upon the prot ... | 1986 | 3021048 |
| establishment of mutant murine mammary carcinoma fm3a cell strains transformed with the herpes simplex virus type 2 thymidine kinase gene. | to establish cell systems appropriate for investigating the mode of action of antiherpetic nucleoside analogues, mutant cell strains were constructed from murine mammary carcinoma fm3a cells, which were deficient in tk, but were transformed with a recombinant plasmid dna containing the hsv-2 tk gene. the transformed cells incorporated the viral dna, expressed viral tk activity and showed unusually high sensitivity to the cytostatic action of the antiherpetic nucleoside analogues acv and ivdu, bo ... | 1986 | 3021345 |
| elisa for detection of igg and igm antibodies to hsv-1 and hsv-2 in human sera. | a rapid, enzyme-linked immunoassay (elisa) was applied to identify and measure specific igg and igm antibodies to herpes simplex viruses types 1 and 2 (hsv-1 and hsv-2). detergent solubilized infected cells and mock-infected cells were used as antigens in the assay. identification of type-specific antibodies was achieved by a competition assay in which clinical sera mixed with hsv-1 or hsv-2 antigens were assayed for reactivity to identical antigens coating wells of polystyrene microtiter plates ... | 1986 | 3021796 |
| a novel glycoprotein for detection of herpes simplex virus type 1-specific antibodies. | a novel herpes simplex virus type 1 (hsv-1)-specific glycoprotein reactive with monoclonal antibody h1379 was purified by affinity chromatography. this glycoprotein, provisionally designated as gg-1, forms two sets of bands with molecular weights of 40-44,000 and 60-88,000. when used in an immunodot enzymatic assay, gg-1 reacted strongly with rabbit antisera to hsv-1, but not with sera hyperimmune to hsv-2. specificity of the assay was further established by the lack of reactivity of convalescen ... | 1986 | 3021801 |
| elisa for detection of group-common and virus-specific antibodies in human and simian sera induced by herpes simplex and related simian viruses. | a rapid (3.5 h) enzyme-linked immunosorbent assay (elisa) was developed for the detection of serum antibodies to herpes simplex virus type 1 (hsv-1) and to two antigenically related monkey viruses, simian agent 8 (sa8) and herpesvirus simiae (b virus). crude preparations of detergent solubilized infected cells and similarly treated control mock-infected cells served as antigens for coating wells in microplates. biotinylated protein a and avidin-conjugated alkaline phosphatase were used to detect ... | 1986 | 3021805 |
| rapid detection of cytomegalovirus in clinical specimens by using biotinylated dna probes and analysis of cross-reactivity with herpes simplex virus. | a method for rapid identification of human cytomegalovirus (hcmv) was developed with biotinylated dna probes. bamhi restriction fragments from hcmv strain ad169 were selected and tested for their ability to detect virus in patient urine samples. all probes detected 30 pg of hcmv ad169 dna. the bamhi b fragment detected 15 of 29 cell-culture-positive samples (sensitivity, 52%). there were four samples which were probe positive and cell culture negative (specificity, 87%). the d and h fragments us ... | 1986 | 3021812 |
| ultraviolet-irradiated urocanic acid suppresses delayed-type hypersensitivity to herpes simplex virus in mice. | ultraviolet radiation is known to induce a transient defect in epidermal antigen presentation which leads to the generation of antigen-specific suppression of the delayed-type hypersensitivity (dth) response. the putative receptor in skin for the primary event in uv-suppression is urocanic acid (uca) which may then interact locally, or systemically, with antigen presenting cells or initiate a cascade of events resulting in suppression. we present the first direct evidence that uca, when irradiat ... | 1986 | 3021864 |
| binding and internalization of herpes simplex virus-antibody complexes by polymorphonuclear leukocytes. | we studied the interactions between rabbit polymorphonuclear leukocytes (pmn) and the re strain of herpes simplex virus type 1 (hsv-1) to determine better the role of inflammatory cells in herpetic stromal keratitis. pmn were found to be nonpermissive for hsv replication and were unable to bind virus in the absence of antibody. however, pmn did bind and internalize hsv-antibody complexes in vitro as was demonstrated visually by electron microscopic studies and quantitatively by measurement of ac ... | 1986 | 3021898 |
| anti-herpes simplex type 1 activity in igg subclasses produced systemically and intrathecally in patients with herpes encephalitis. | the role of the humoral immune response in herpes simplex encephalitis (hse) is largely unknown. the finding that herpes simplex virus type 1 (hsv 1) induced igg fc receptor binds to all igg subclasses except igg 3 prompted an investigation of anti-hsv activity in igg subclasses from serum and cerebrospinal fluid (csf) in ten patients with proven or highly probable hse by means of a monoclonal antibody igg subclass-specific solid-phase radioimmunoassay (spria). in contrast to serum, csf containe ... | 1986 | 3021917 |
| binding of complement component c3b to glycoprotein gc of herpes simplex virus type 1: mapping of gc-binding sites and demonstration of conserved c3b binding in low-passage clinical isolates. | the sites on glycoprotein gc of herpes simplex virus type 1 (hsv-1) which bind complement component c3b were evaluated by using anti-gc monoclonal antibodies and mutants which have alterations at defined regions of the glycoprotein. monoclonal antibodies were incubated with hsv-1-infected cells in a competitive assay to block c3b binding. each of 12 different monoclonals, which recognize the four major antigenic sites of gc, completely inhibited c3b binding. with this approach, no one antigenic ... | 1986 | 3021981 |
| the promoter for the late gene encoding vp5 of herpes simplex virus type 1 is recognized by cell extracts derived from uninfected cells. | the ability of whole-cell extracts from uninfected hela cells to recognize the promoter for the herpes simplex virus type 1 late gene encoding the major capsid protein vp5 was investigated by using both in vitro transcriptional and s1 nuclease protection analysis. this gene promoter was recognized by the cell extracts and produced abundant amounts of transcript in the absence of any other virus-encoded factors. this transcript was shown to arise, in vitro, from specific initiation at or very nea ... | 1986 | 3021995 |
| construction and properties of a cell line constitutively expressing the herpes simplex virus glycoprotein b dependent on functional alpha 4 protein synthesis. | we report the construction of a cell line constitutively expressing the glycoprotein b (gb) of herpes simplex virus (hsv) 1. the cell line was constructed in two steps. in the first, a baby hamster kidney cell line was transfected with the dna of a plasmid containing the neomycin phosphotransferase gene that confers resistance to the antibiotic g418 and the gene specifying a temperature-sensitive (ts-) alpha 4 protein of hsv-1, the major viral regulatory protein. a clonal cell line, alpha 4/c113 ... | 1986 | 3022001 |
| construction and properties of a viable herpes simplex virus 1 recombinant lacking coding sequences of the alpha 47 gene. | the five alpha genes, alpha 0, alpha 4, alpha 22, alpha 27, and alpha 47, are the first set of herpes simplex virus 1 genes to be transcribed and expressed in productively infected cells. we report here the construction of a viral recombinant from which all of the coding sequences of the alpha 47 gene were deleted. in addition to the alpha 47 protein, infected cell lysates did not contain detectable amounts of two polypeptide bands with apparent molecular weights of 18,000 and 21,000 which could ... | 1986 | 3022015 |
| [comparison of complement fixation and tzanck smear tests for the diagnosis of herpes labialis]. | in this study, the serum antibody titer of herpes simplex virus type 1 (hsv-1) in 82 patients with herpes labialis and 100 controls was determined using the complement fixation test. in own experience, the tzanck smear test (reported to be an aid in the diagnosis of hsv infection) was of no value. in comparison to patients in an early stage of infection, convalescent patients had an antibody titer that was 4 or more times higher (1/64-1/256). fifty-eight percent of the control group was negative ... | 1986 | 3022117 |
| nucleotide sequence of the dna polymerase gene of herpes simplex virus type 1 strain angelotti. | 1986 | 3022246 | |
| two avirulent herpes simplex viruses generate lethal recombinants in vivo. | while it is widely appreciated that infection with a virulent virus can produce disease in an animal, the ability of a mixture of avirulent viruses to produce disease by means of complementation or recombination in vivo has not been established. in this study, two weakly neuroinvasive herpes simplex virus type 1 (hsv-1) strains were simultaneously inoculated onto the footpads of mice. many (62%) of the animals that received a 1:1 mixture of the viruses died, whereas the animals that received a s ... | 1986 | 3022376 |
| [herpes simplex virus 1 and herpes labialis]. | 1986 | 3022404 | |
| sequences homologous to two separate transforming regions of herpes simplex virus dna are linked in two human genital tumors. | ten human genital invasive squamous cell carcinomas and five human premalignant tissues were analyzed for the presence of selected sets of herpes simplex virus 2 (hsv-2) dna sequences. two vulvar tumors and one vulvar dysplastic tissue were found to contain dna sequences homologous to the bglii o fragment (coordinates 0.38-0.42) and the bglii n fragment (coordinates 0.58-0.63) of hsv-2 dna. these two fragments overlap the subsets of hsv-1 and hsv-2 dna sequences (respectively) shown previously t ... | 1986 | 3022470 |
| thymidine kinase (tk) activity in herpes simplex virus type 1 recombinants that carry insertions affecting regulation of the tk gene. | determinations of the possible importance of herpes simplex virus type 1 (hsv) thymidine kinase (tk) expression in the pathogenesis of viral latency depend in part on the use of defined mutants. in a recent study by a. e. sears, b. meignier, and b. roizman (j. virol. 55, 410-416 (1985], in which they utilized genetically engineered viral recombinants considered to be tk-, the role of hsv tk expression in latency was reported to be minimal. to further investigate this conclusion we intensively st ... | 1986 | 3022474 |
| a herpes simplex virus mutant is temperature sensitive for reactivation from the latent state: evidence for selective restriction in neuronal cells. | when the replicative defect in the hsv-1 temperature sensitive mutant tsi was repaired, the agent derived (ri-1) was found to possess an additional temperature sensitive lesion limiting its reactivation from the latent state. thus, when spinal ganglia from latently infected mice were scored for reactivation by cocultivating them with indicator cells in vitro, significantly more were found to be positive at 31 degrees than at 38.5 degrees. to assess a possible relationship between reactivation an ... | 1986 | 3022476 |
| efficacy of 2'-nor-cyclicgmp in treatment of experimental herpes virus infections. | 9-[(2-hydroxy-1,3,2-dioxaphosphorinan-5-yl)oxymethyl]guanine p-oxide (2'-nor-cgmp), the cyclic phosphate of 2'-nor-deoxyguanosine (2'-ndg) was synthesized by phosphorylation of 2'-ndg and evaluated for antiherpetic activity in cell cultures and in animal protection studies. 2'-nor-cgmp was effective in cell culture against both thymidine kinase deficient and wild-type herpes simplex virus type 1 strains and also against herpes simplex virus type 2. the anti-herpes activity of 2'-nor-cgmp against ... | 1986 | 3022643 |
| characterization of a herpes simplex virus regulatory protein: aggregation and phosphorylation of a temperature-sensitive variant of icp 4. | the viral polypeptide icp 4 (or vmw 175) is synthesized during the immediate early phase of infection by herpes simplex virus (hsv) and is required during the viral reproductive cycle for efficient transcription of delayed early viral genes. replication of mutant strains of hsv-1 such as tslb 2 that encode a temperature-sensitive variant of icp 4 does not proceed beyond the immediate early phase in cells that are infected and maintained at the nonpermissive temperature (npt). under these conditi ... | 1986 | 3022682 |
| skin langerhans cells play an essential role in the defense against hsv-1 infection. | the role of the langerhans cells (lc) during hsv-1 infection in murine skin was examined. lc function as very potent antigen-presenting cells and represent the most peripheral immune cellular elements in the body. the footpad route was used to study the response of lc to infection with a pathogenic and non-pathogenic strain of hsv-1. following infection with a pathogenic hsv-1 strain, there was an increase in the number of lc in the footpad skin. depletion of lc from the skin by treatment with 1 ... | 1986 | 3022684 |
| neurobiology of human herpesvirus infections. | critical to the adaptation of herpes simplex virus type 1 (hsv-1) to its human host is a complex interaction with the peripheral nervous system. the "life cycle" of the virus depends not only on axoplasmic transport which carries virus to and from the infected ganglia, but also on variable expression of the virus within ganglionic neurons. during latency, limited viral gene expression allows the virus to persist undetected by immune defenses, while intermittent reactivation, perhaps triggered by ... | 1986 | 3022987 |
| ultrastructure of antibody dependent cellular cytotoxicity in hsv 1 infected and uninfected chang liver cells. | the binding and ultrastructural features of antibody dependent cellular cytotoxicity (adcc) mediated by human peripheral blood lymphocytes were studied in herpes simplex virus type i (hsv-1) infected chang liver (cl) cells plus human anti-hsv-1 serum, and in uninfected cl cells plus guinea pig anti-cl antiserum. non-cytolytic controls included target cells treated with normal serum in place of sensitized targets and heat shocked lymphocytes instead of normal lymphocytes. by transmission electron ... | 1986 | 3022993 |
| type specificity of complement-fixing antibody against herpes simplex virus type 2 ag-4 early antigen in patients with asymptomatic infection. | we evaluated the type specificity of complement-fixing (cf) antibody against the ag-4 early antigen of herpes simplex virus (hsv) type 2 (hsv-2) by comparing a commercial ag-4 cf kit (simplex-2; gene link australia, inc., princeton, n.j.) with quantal microneutralization (mn) and absorption-western blotting in testing sera from patients with and without a history of genital herpes. sera characterized as hsv type 1 (hsv-1) or hsv-2 positive or negative by mn were selected and tested by cf, and th ... | 1986 | 3023439 |
| selection and characterization of an interferon-responsive clonal cell line of hela cells. | hela cells generally do not respond well to interferon (ifn). we have used is-1, an ifn-sensitive mutant of mengovirus, to select a clone of ifn-responsive hela cells (f-h12). at moderate levels of human alpha/beta ifn, is-1 yields were fivefold lower in these cells than in similarly protected control cells. in contrast, wild-type mengovirus, vesicular stomatitis virus and a wild-type and thymidine kinase-negative strains of herpes simplex virus type 1 grew equally well in both cell lines. by a ... | 1986 | 3023528 |
| characterization of the ie110 gene of herpes simplex virus type 1. | we have determined the dna sequence of the herpes simplex virus type 1 (hsv-1) gene encoding the immediate early protein ie110, which is involved in transcriptional activation of later virus genes. the locations of the 5' and 3' termini of ie110 mrna together with the positions of two introns, were identified. examination of the dna sequence suggested that translation starts at the first atg after the 5' terminus of the mrna, and that both introns occur in protein-coding sequence. the predicted ... | 1986 | 3023529 |
| restoration of wild-type pathogenicity to an attenuated dna polymerase mutant of herpes simplex virus type 1. | the drug-resistant variant, rsc-26, which was derived from the herpes simplex virus type 1 wild-type strain sc16, expresses an altered dna polymerase and has reduced pathogenicity in animal models. to determine whether the attenuation in pathogenicity was due solely to mutation in the polymerase gene, a fragment of the wild-type gene was cloned, transferred into the genome of rsc-26 and recombinants were isolated. three recombinants examined had similar properties to wild-type virus with respect ... | 1986 | 3023535 |
| the products of herpes simplex virus type 1 (hsv-1) immediate early genes 1, 2 and 3 can activate hsv-1 gene expression in trans. | expression of the early and late genes of herpes simplex virus type 1 (hsv-1) during infection of tissue culture cells requires the prior expression of the immediate early (ie) genes. the requirement for the product of ie gene 3, vmw175, for the activation of early promoters has been revealed by studies with temperature-sensitive virus mutants. recent experiments using transfection assays have shown that both vmw175 and the product of ie gene 1, vmw110, are involved in the transactivation of a v ... | 1986 | 3023536 |
| direct inactivation of viruses by human granulocyte defensins. | human neutrophils contain a family of microbicidal peptides known as defensins. one of these defensins, human neutrophil peptide (hnp)-1, was purified, and its ability to directly inactivate several viruses was extensively tested. herpes simplex virus (hsv) types 1 and 2, cytomegalovirus, vesicular stomatitis virus, and influenza virus a/wsn were inactivated by incubation with hnp-1. two nonenveloped viruses, echovirus type 11 and reovirus type 3, were resistant to inactivation. purified homolog ... | 1986 | 3023659 |
| transcription initiation sites and nucleotide sequence of a herpes simplex virus 1 gene conserved in the epstein-barr virus genome and reported to affect the transport of viral glycoproteins. | earlier reports have localized mutations which affect the processing and transport of herpes simplex virus 1 glycoproteins to a region located between the genes specifying glycoprotein b and the major viral dna-binding protein (beta 8). the nucleotide sequence of this region contains a single long open reading frame encoding a 780-amino-acid protein with a predicted molecular weight of 83,845. to confirm the existence of this protein, rabbit polyclonal antibody was made against a synthetic pepti ... | 1986 | 3023664 |
| high voltage electron microscopy of whole cells infected with herpes simplex virus type 1. | 1986 | 3027223 | |
| effect of antiviral agents on replication of herpes simplex virus type 1 in brain cultures. | an in vitro tissue culture system consisting of reaggregated embryonic brain cells was used to evaluate the inhibition of herpes simplex type 1 (hsv-1) by several antiviral compounds. the efficacy of acyclovir, vidarabine, bromovinyldeoxyuridine, and 9-(1,3-dihydroxy-2-propoxymethyl) guanine in hsv-1-infected vero cell monolayer cultures was compared with that seen with brain cell aggregates. at a mean 50% inhibitory dose with vero cells, acyclovir showed a 99% reduction of virus titer in brain ... | 1986 | 3028250 |
| suppression of matrix protein synthesis by herpes simplex virus type 1 in human endothelial cells. | the synthesis of matrix proteins was investigated in cultures of human umbilical vein endothelial cells (ec) infected with herpes simplex virus type 1 (hsv-1). ec cultures were either mock-infected or infected for 1 hour at a multiplicity of infection (moi) of 5 or 20 infectious particles per cell. synthesis was followed by determining [14c]-proline or [35s]-methionine incorporation into non-dialyzable proteins. using sds-page we observed that synthesis of fibronectin (fn), type iv procollagen a ... | 1986 | 3028708 |
| prevalence of htlv iii/lav and other viral antibodies in normal and at risk populations. | the prevalence of htlv iii/lav antibody has been studied in six groups of population: healthy subjects, a virological laboratory staff, prisoners, drug addicts, patients with lymphoadenopathy-associated syndrome (las) and patients with aids. prevalences and levels in the same groups of hbsag and cmv, hsv 1 and hsv 2 antibodies have been studied, too. as expected, the prevalence of htlv iii/lav antibody increases in the six groups with the increasing of the risk while the prevalences of the other ... | 1986 | 3030363 |
| immunological studies of uveitis. 1. immune complex containing herpes virus antigens in four patients with acute retinal necrosis syndrome. | four patients with acute retinal necrosis syndrome were examined. three of them showed elevated antibody titers to varicella-zoster virus during the convalescent stage, while one of them showed an elevated titer to herpes simplex virus type 1, especially in the intraocular fluid. also, these patients had an elevated circulating immune complex in the acute phase. western blotting demonstrated that the immune complexes contained varicella-zoster or herpes simplex type 1 antigen, corresponding to t ... | 1986 | 3035258 |
| herpes simplex type ii encephalitis in infancy presenting with focal encephalitis. | herpes virus was recovered from a throat swab, nasopharyngeal washings as well as from brain tissue from a six-month-old infant, who presented with fever, left focal seizures and an enhancing right frontal ct-scan lesion. cytopathic effect (cpe) as seen with genital herpetic infection was seen, suggesting hsv-2. immunofluorescent typing of the virus isolate confirmed hsv-2. early igm positivity preceding a cf and sn titer rise was observed. the patient received a course of ara-a and recovered wi ... | 1986 | 3027605 |
| oral cancer induced in hamsters with herpes simplex infection and simulated snuff dipping. | a number of epidemiologic studies indicate that snuff dipping is associated with an increased incidence of oral cancer in human beings. since inactivated herpes simplex virus (hsv) has been shown to induce malignant changes in vitro and in vivo and is partially inactivated by snuff water extract, we examined the histopathologic changes of hamster buccal pouches after exposure to repeated hsv inoculation combined with long-term simulated snuff dipping. one hundred twenty-five syrian hamsters were ... | 1986 | 3462612 |
| a new look at experimental herpes simplex eye disease: preliminary results of clinical disease in the nih mouse after zosteriform spread. | following the discovery by tullo et al that herpes simplex virus type 1 (hsv1) can reach the eye by zosteriform spread after inoculation into the cutaneous field of the trigeminal ganglion, this model has been adapted to the nih strain of inbred mouse. clinical disease in the nih mouse after snout inoculation includes skin disease, mydriasis, iritis and keratitis. | 1986 | 3466461 |
| viral alkaline nuclease in intranuclear dense bodies induced by herpes simplex infection. | the distribution of the herpes simplex virus alkaline nuclease (hsv dnase) in rabbit fibroblast cells infected with hsv type 1 or 2 (hsv-1 or hsv-2) has been examined with the aid of immunocytochemical techniques using gold particles as markers. hsv dnase was found to be accumulated within infected nuclei as early as 2.5 hr post-infection. labeled antibody to hsv dnase subsequently increased in all nuclei after 7 hr and 17 hr. at all times post-infection the virus induced nuclear dense bodies we ... | 1986 | 3032315 |
| herpes simplex virus gene products involved in the induction of chromosomal aberrations. | the effect of short-term herpes simplex virus type 1 (hsv-1) infection on chromosomes of human diploid fibroblasts was examined. in addition to chromosomal breaks, gaps and pulverization, three kinds of cytogenetic damage (double minutes, polyploidy and endoreduplication) not yet reported following productive infection with hsv or other animal viruses were frequently observed. consistent with previous studies suggesting that the expression of immediate-early and/or early viral gene products is r ... | 1986 | 3033932 |
| herpes simplex virus, cytomegalovirus and epstein-barr virus antibody titres in sera from schizophrenic patients. | serum antibody titres to herpes-simplex (hsv-1, 2), cytomegalovirus (cmv), and epstein-barr virus capsid antigen (ebv-vca) were determined in 38 unrelated chronic schizophrenic patients, 11 nuclear families with at least 2 schizophrenic members, and 2 control groups. the distributions of antibody titres to herpes simplex and cytomegalovirus were similar among all groups. patients had higher anti-ebv-vca titres than non-hospitalized controls; however, hospital staff members in contact with the pa ... | 1986 | 3029788 |
| efficacy of topical treatment for herpes simplex virus infections: predictions from an index of drug characteristics in vitro. | topical antiviral treatments for recurrent infection with herpes simplex virus in immunocompetent patients have been generally ineffective. we investigated whether in vitro drug measures could predict in vivo efficacy. twelve topical antiviral formulations were evaluated in vitro by measuring inhibition of viral plaque formation in cell culture (id50) and drug penetration through excised guinea pig skin. in vivo efficacy for each treatment was determined in an experimental cutaneous infection wi ... | 1986 | 3079810 |
| efficacy of glycoprotein inhibitors alone and in combination with trifluridine in the treatment of murine herpetic keratitis. | the present study examined the anti-herpetic effect of the glycoprotein inhibitors, hydroxynorvaline and 2-deoxyglucose, alone and in combination with trifluridine on murine ocular herpes. following ocular inoculation with a large dose of hsv-1 re strain (10(6) pfu), icr mice were treated during the acute infection with different therapeutic regimens, and their efficacy was evaluated by ocular virus titers, clinical grading of blepharo-conjunctivitis and histological evaluation of stromal kerati ... | 1986 | 3082600 |
| incidence of herpes genitalis in northern ireland in 1973-83 and herpes simplex types 1 and 2 isolated in 1982-4. | during 1973 - 83 there was a pronounced rise in the number of new cases of herpes genitalis in northern ireland. this study of 127 patients with herpes genitalis shows that 30% had herpes simplex virus type 1 (hsv 1) and 70% had herpes simplex virus type 2 (hsv 2) infections. of 41 sexual contacts who attended, only seven had herpes genitalis (caused in each case by hsv 2); another patient had a history of genital lesions, and one other of oral lesions, but hsv was not isolated from either patie ... | 1986 | 3949348 |
| ocular toxicity of intravitreal vidarabine solubilized in dimethyl sulfoxide. | intravitreal injections of the antiviral drug vidarabine in doses of 10, 50, 100, and 200 micrograms dissolved in 100% dimethyl sulfoxide (dmso) were administered to the eyes of 12 dutch and new zealand pigmented rabbits to determine ocular toxicity. the eyes were examined one week, one month, and two months after inoculation with slit-lamp biomicroscopy, indirect ophthalmoscopy, electroretinography, and histopathologic examination with light and electron microscopy. no permanent damage to ocula ... | 1986 | 3954645 |
| oral bromovinyldeoxyuridine therapy for herpes simplex and varicella-zoster virus infections in severely immunosuppressed patients: a preliminary clinical trial. | twenty-five patients with haematological diseases were treated orally with the highly potent and selective anti-herpes agent, bromovinyldeoxyuridine (bvdu), in a dosage of 7.5 mg/kg/day (divided over three or four doses a day) for 5 days for an intercurrent mucocutaneous herpesvirus infection. of these 25 patients, 8 were severely granulocytopenic at the time of the viral infection, and 12 recently had undergone bone-marrow transplantation; 5 were under cytotoxic therapy for a lymphoproliferativ ... | 1986 | 3511181 |
| oral acyclovir (zovirax) in herpes simplex dendritic corneal ulceration. | sixty patients with simple dendritic corneal ulceration were randomly assigned to double blind treatment with either acyclovir tablets (400 mg) or acyclovir ophthalmic ointment administered five times daily. there was no significant difference in the proportions of patients healed in either treatment group (88.9% on oral acyclovir and 96.6% on acyclovir ointment). the median healing time was five days in both groups. no systemic or significant local side effects were noted in either treatment gr ... | 1986 | 3521717 |
| adenovirus replication as an in vitro probe for drug sensitivity in human tumors. | the feasibility of using adenovirus 5 as an in vitro probe for chemosensitivity in short-term cultures of human tumors was evaluated using human melanoma cell lines and primary cultures of melanoma biopsies. a convenient immunoperoxidase method was developed for quantitating viral replication 2 days after infection. two different approaches were explored: the host cell reactivation assay (hcr) using drug-treated virus; and the viral capacity assay using drug-treated cells. the hcr assay detected ... | 1986 | 3525182 |
| synthesis of fused glycoprotein d of herpes simplex virus type 1 but not type 2 inhibits escherichia coli hosts. | glycoprotein d from either herpes simplex virus type 1 (gd-1) or type 2 (gd-2) has been expressed in escherichia coli as a series of chimeric proteins. the expression vector used in this study, pjs413, was derived from pbr322 and contains several cloning sites between the lacz promoter-operator and the phage lambda cro gene. plasmids containing fusions between the cro gene, gd-related sequences and lacz was constructed and shown to direct the synthesis of 160-kda proteins. the accumulation of fu ... | 1986 | 3527874 |
| detection of viral antigens in cerebrospinal fluid of rabbits with experimental herpes simplex virus type 1 encephalitis. | we investigated several methods for the rapid diagnosis of herpes simplex virus induced encephalitis in a rabbit model. the corneas of twenty-two rabbits were infected with herpes simplex virus type 1 (hsv-1) and diagnosis of herpes encephalitis was made by virus isolation, immunofluorescent and peroxidase staining of brain biopsies, demonstration of anti-hsv igm in cerebrospinal fluid (csf) and by an indirect enzyme-linked immunosorbent assay (elisa), designed for detection of viral antigens. w ... | 1986 | 3530193 |
| high-performance liquid chromatographic analysis of (e)-5-(2-bromovinyl)-2'-deoxyuridine and its metabolites in serum, urine and herpes simplex virus type-1 infected cells. | 1986 | 3700568 | |
| response of dairy calves to vaccinia viruses that express foreign genes. | repeated intradermal inoculations of calves with wild-type vaccinia virus and recombinant vaccinia viruses expressing human hepatitis b virus surface antigen and herpes simplex virus, type 1, glycoprotein d produced characteristic pox lesions at each site of injection. in some instances, calves were inoculated as many as five times at intervals from 4 to 7 weeks. the lesions invariably were more severe after the second inoculation. subsequent inoculations produced a less severe area of redness, ... | 1986 | 3700615 |
| pharmacokinetics and metabolism of e-5-(2-[131i]iodovinyl)-2'-deoxyuridine in dogs. | e-5-(2-iodovinyl)-2'-deoxyuridine (ivdu) is a potent inhibitor of herpes simplex virus type 1 replication in vitro. the selective antiviral activity of ivdu is due to preferential phosphorylation by the herpes simplex virus type 1-encoded thymidine kinase. this selective sequesteration provided the rationale for the development of radioiodinated ivdu as a potential radiopharmaceutical compound for use in noninvasive diagnosis of herpes simplex virus encephalitis. we studied the pharmacokinetics ... | 1986 | 3717935 |
| [plasma exchange and acyclovir in behçet's disease]. | it has been postulated that behçet's disease may be a viral induced vasculitis. in the present study plasma exchanges were applied in an attempt to remove circulating immune complexes and restore cellular immunity. high doses of acyclovir were administered in association with plasma exchanges since it has been shown that herpes simplex virus type 1 might be involved in behçet's vasculitis. seven patients with severe ocular manifestations of behçet's disease were selected for the study. plasma ex ... | 1986 | 3722720 |
| herpes simplex encephalitis in infants. | two cases are reported of severe encephalitis in infants that were caused by a primary infection with herpes simplex virus type 1. both patients presented with focal seizures; the clinical diagnosis was confirmed by the rapid appearance of igm antibody to herpes simplex virus type 1 in cerebrospinal fluid and serum. in spite of the early use of acyclovir, which was administered by the intravenous route, the clinical response was poor and both infants were left with severe neurological impairment ... | 1986 | 3724604 |
| exposure to low-dose ultraviolet radiation suppresses delayed-type hypersensitivity to herpes simplex virus in mice. | ultraviolet b (uvb) radiation is reported to induce a defect in epidermal antigen presentation which leads to specific suppression of the delayed-type hypersensitivity (dth) response to trinitrochlorobenzene. we have used a similar system to examine the murine dth response to herpes simplex virus type 1 (hsv-1). mice irradiated with 96 mj/cm2 uvb on shaved dorsal skin 3 days before s.c. injection of live hsv-1 in the flank showed 54-92% suppressed dth responses to challenge with inactivated viru ... | 1986 | 3745940 |
| potential of bromovinyldeoxyuridine in anticancer chemotherapy. | bromovinyldeoxyuridine (bvdu) is a highly potent and selective antiherpetic agent which offers great potential for the treatment of severe herpes simplex virus type 1 (hsv-1) and varicella-zoster virus (vzv) infections in cancer patients. bvdu inhibits the replication of hsv-1 and vzv at a concentration as low as 1-10 ng/ml; and the proliferation of tumor cells transformed with the hsv-1 thymidine kinase gene is even inhibited by bvdu concentrations lower than 1 ng/ml. moreover, bvdu is inhibito ... | 1986 | 3752935 |
| ocular penetration and efficacy of chloroethyldeoxyuridine against herpetic keratouveitis. | topical administration of 0.1% or 1% chloroethyldeoxyuridine eyedrops caused a significant reduction in the severity of experimental herpes simplex iritis and stromal keratitis in rabbits. the healing effect caused by chloroethyldeoxyuridine was comparable to that obtained with 0.1% and 0.5% bromovinylde-oxyuridine eyedrops. the drug levels achieved in the anterior chamber fluid following topical application of 1% eyedrops of [2-14c]cedu, a radiolabelled analogue of chloroethyldeoxyuridine, were ... | 1986 | 3759363 |