Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| mycobacterium bovis infections in wild ferrets. | 1993 | 16031717 | |
| retinal projections to the pretectum, accessory optic system and superior colliculus in pigmented and albino ferrets. | retinal projections to the pretectal nuclei, accessory optic system and superior colliculus in pigmented and albino ferrets were studied using anterograde tracing techniques. both nissl- and myelin-stained material was used to identify the pretectal nuclei, nuclei of the accessory optic system and the layers of the superior colliculus. following monocular injection of either horseradish peroxidase or rhodamine-b-isothiocyanate, four pretectal nuclei, including the nucleus of the optic tract, pos ... | 1993 | 8261124 |
| anti-emetic profile of a non-peptide neurokinin nk1 receptor antagonist, cp-99,994, in ferrets. | in the ferret, 5-ht3 receptor antagonists are effective in controlling emesis produced by cytotoxic agents or radiation. to investigate the possibility that substance p has a role, as well as 5-ht, in the emetic reflex pathway, we have examined the anti-emetic effects of a nk1 receptor antagonist (racemic cp-99,994) in the ferret. racemic cp-99,994 was effective against a range of emetogens, comprising cytotoxic drugs, radiation, morphine, ipecacuanha and copper sulphate. | 1993 | 7506663 |
| a method for determining the activity state of hair follicles. | a histological method is described for determining the proportion of growing hair follicles in skin samples. a variation of the sacpic staining method, modified for bulk processing, produces high contrast staining of the principal tissue types present in skin. in particular, the inner root sheath is accentuated, facilitating detection of active follicles. skin preparations from a range of species are used to illustrate structural characteristics of follicles viewed in cross section at various st ... | 1993 | 7507355 |
| the role of endocardial endothelium in the modulation of myocardial contraction in the isolated whole heart. | selective damage to the endocardial endothelium in isolated papillary muscle preparations has been shown to produce an abbreviation of contraction, changes which were also observed following an increase in myocardial cyclic gmp in those preparations. in the present study we have investigated the effects of removing the endocardium and increasing myocardial cyclic gmp on contractile parameters in isolated langendorff perfused ferret hearts, where the myocardial mass underlying the endocardium is ... | 1993 | 7507721 |
| photostimulation using caged glutamate reveals functional circuitry in living brain slices. | an approach for high-spatial-resolution mapping of functional circuitry in living mammalian brain slices has been developed. the locations of neurons making functional synaptic connections to a single neuron are revealed by photostimulation of highly restricted areas of the slice (50-100 microns in diameter) while maintaining a whole-cell recording of the neuron of interest. photostimulation is achieved by bathing brain slices in a molecularly caged form of the neurotransmitter glutamate [l-glut ... | 1993 | 7689225 |
| trigeminal nuclear complex of the ferret: anatomical and immunohistochemical studies. | in order to establish the ferret as an animal model for studies of trigeminal pain, we describe the cytoarchitecture and neurochemistry of the trigeminal nuclear complex in the ferret and compare them to those of the cat and rat. the complex was divided as previously described, but the ferret differed in the extent of the nuclear boundaries. the neuroanatomical istribution of substance p-, calcitonin gene-related peptide-, galanin-, enkephalin-, serotonin-, somatostatin-, neuropeptide y-, and ne ... | 1993 | 7681453 |
| the central localization of the vagus nerve in the ferret (mustela putorius furo) and the mink (mustela vison). | the location of vagal preganglionic neurones (vpn) has been determined in nine ferrets (mustela putorius furo) and seven mink (m. vison) using neuronal tract-tracing techniques employing horseradish peroxidase (hrp) and wheat-germ agglutinin conjugated hrp (wga-hrp) mixtures injected into the nodose ganglion of the vagus nerve. labelled vpn were located ipsilaterally in the dorsal motor nucleus of the vagus (dmnx), nucleus ambiguus (na), and reticular formation (rf) of the medulla oblongata. in ... | 1993 | 7686926 |
| ferret tracheal mucus rheology, clearability and volume following administration of substance p or methacholine. | we studied the effects of substances p administration on tracheal mucus viscoelasticity, water content, clearability and secretion rate. six neutered adult male ferrets (weight 1.1-1.5 kg) were studied, on four occasions each. they were anaesthetized with ketamine and xylazine, and intubated shallowly. control mucus (pre- and post-ringer instillation) was compared with the mucus obtained following instillation of 200 microliters of 10(-6), 10(-5), and 10(-4) m substance p (sp), and 10(-4) m meth ... | 1993 | 7678818 |
| tachykinin control of ferret airways: mucus secretion, bronchoconstriction and receptor mapping. | the effects of synthetic tachykinin receptor agonists on mucus secretion by ferret trachea was determined in vitro in ussing chambers using 35so4 as a mucus marker and the synthetic peptides [sar9,met(o2)11]substance p (sarsp), [beta ala8]neurokinin a-(4-10) and [mephe7] neurokinin b which are selective for nk1, nk2 and nk3 tachykinin-receptors respectively. the bronchomotor effects of the same agonists were also studied in vitro and tachykinin receptors were localized by autoradiographic mappin ... | 1993 | 7681552 |
| cervical chordoma in two ferrets (mustela putorius furo). | 1993 | 7682370 | |
| aleutian disease in domestic ferrets: diagnostic findings and survey results. | aleutian disease was diagnosed as the cause of posterior ataxia and paresis in domestic ferrets. six serologically positive animals (four clinically affected and two unaffected) were investigated in detail and seven other clinically affected ferrets were also identified. the diagnostic findings included hypergammaglobulinaemia, histological lesions in the central nervous system and parvovirus-like particles in mesenteric lymph nodes. a wider serological survey of 446 animals owned by members of ... | 1993 | 7685128 |
| comparison of muscle cell fiber types and oxidative capacity in gracilis, rectus femoris, and triceps brachii muscles in the ferret (mustela putorius furo) and the domestic dog (canis familiaris). | muscle cell fiber types in gracilis, rectus femoris, and long head of triceps brachii muscles of ferrets and dogs were identified on serial sections stained for myosin atpase after preincubation at ph values of 9.8, 4.6, and 4.3 and for nadh-tetrazolium reductase (nadh-tr) activity. although fiber types i and ii were identified, the atpase stain did not demonstrate classic type iia/iib fiber differences in either species. however, two type ii fiber subtypes could be distinguished in the ferret b ... | 1993 | 7691036 |
| regional content of enteric substance p and vasoactive intestinal peptide during intestinal inflammation in the parasitized ferret. | our aim was to characterize and quantitate changes in two key neuropeptides, substance p (sp) and vasoactive intestinal peptide (vip), that are involved in governing neurally-mediated gastrointestinal (gi) reflex activity during enteric inflammation in the ferret. neuropeptide content was determined by radioimmunoassay of extracts of jejunal, ileal and colonic muscularis externa from uninfected ferrets and ferrets infected with enteric stages of the parasitic nematode, trichinella spiralis. incr ... | 1993 | 7692345 |
| colocalization of vasoactive intestinal peptide and nitric oxide synthase in neurons of the ferret trachea. | neurally-mediated relaxation of smooth muscle in human, guinea-pig, cat, and pig airways is largely attributed to a nonadrenergic, noncholinergic mechanism. while the specific transmitter(s) of this relaxant system have not been conclusively identified, vasoactive intestinal peptide and nitric oxide have emerged as likely mediators in airway smooth muscle. both vasoactive intestinal peptide and nitric oxide relax guinea-pig, pig and human smooth muscle. vasoactive intestinal peptide is present i ... | 1993 | 7688104 |
| characterization of tachykinin receptors in ferret trachea by peptide agonists and nonpeptide antagonists. | the tachykinin receptors mediating mucus secretion and smooth muscle contraction were studied in the ferret trachea in vitro. substance p (sp) and the selective agonist for nk1 receptor ([sar9,met(o2)11]sp), but not selective agonists for nk2 ([ala5,beta-ala8]neurokinin a-(4-10)) and nk3 ([mephe7]neurokinin b) receptors, induced secretion of macromolecules in a concentration-dependent fashion. the nonpeptide nk1 receptor antagonist, cp-96,345, but not the nonpeptide nk2 receptor antagonist, sr-4 ... | 1993 | 7690189 |
| neuromodulation of calcium current by extracellular atp in isolated ventricular myocytes. | the effects of extracellular atp on the l-tpe ca2+ current (ica), action potential, and resting and intracellular ca2+ levels were examined in enzymatically isolated myocytes from the right ventricles of ferrets. extracellular atp decreased the peak amplitude of ica in a time- and concentration-dependent manner. the concentration-response relationship for atp inhibition of ica was well described by a conventional michaelis-menten relationship with a half maximal inhibitory concentration of 1 mic ... | 1993 | 7910429 |
| cortical specification: microcircuits, perceptual identity, and an overall perspective. | the microcircuitry within a cortical area and its perceptual identity are both specified relatively late in development. we have asked whether and how the pattern of input activity during development influences these features of cortex. routing visual projections to the auditory pathway in ferrets leads to visual activation of the developing auditory cortex, causing auditory cortex to receive patterns of input activity very different from normal. visual inputs respecify, in instructive fashion, ... | 1993 | 8087534 |
| tension and intracellular calcium transients of activated ferret ventricular muscle in response to step length changes. | to elucidate the effects of mechanical constraints on the (ca2+) affinity of cardiac troponin c, we studied the relationships among the myoplasmic ca2+ concentration ([ca2+]i), tension and length in steadily activated intact cardiac muscle. the ca2+ sensitive photoprotein, aequorin, was micro-injected into cells of ferret right ventricular papillary muscles to monitor the [ca2+]i. the muscle was then steadily activated with ouabain (10(-4) m)(ouabain contracture) or high frequency stimuli in the ... | 1993 | 8109375 |
| the role of na(+)-ca2+ exchange in paired pulse potentiation of ferret ventricular muscle. | 1. stimulation of cardiac muscle with pairs of stimuli ('paired pulse stimulation') results in a large inotropic effect and experiments have been carried out on ferret ventricular muscle to investigate the underlying mechanism. 2. aequorin was used to measure sarcoplasmic ca2+ in papillary muscles. during paired pulse stimulation the first aequorin light transient (i.e. ca2+ transient) and contraction of the pair increased in amplitude, whereas the second aequorin light transient and contraction ... | 1993 | 8145152 |
| regulation of intracellular calcium in cardiac muscle. | we have investigated the regulation of intracellular free calcium in heart muscle using the free acid form of the ca2+ indicator fura-2 iontophoretically microinjected into rat cardiac trabeculae or ferret papillary muscles. this method shows great promise in elucidating a number of crucial questions in cardiac excitation-contraction coupling. | 1993 | 8184768 |
| immunolocalization of scrapie amyloid in non-congophilic, non-birefringent deposits in golden syrian hamsters with experimental transmissible mink encephalopathy. | transmissible mink encephalopathy (tme), a naturally occurring subacute spongiform encephalopathy in commercially ranch-reared mink (mustela vision), is characterized neuropathologically by spongiform changes in the neuropil, intracytoplasmic neuronal vacuolation and astrocytic hypertrophy and hyperplasia. amyloid deposits have not been observed in brain tissue sections from animals with natural and experimental tme using conventional histochemical stains such as congo red. to determine if amylo ... | 1993 | 8103204 |
| the effects of orally administered y-25130, a selective serotonin3-receptor antagonist, on chemotherapeutic agent-induced emesis. | the antiemetic effects of orally administered y-25130, a potent and selective 5-ht3-receptor antagonist, were compared with those of ondansetron, granisetron, metoclopramide and domperidone. y-25130 (0.1-1.0 mg/kg) dose-dependently prolonged the latency to the first vomiting and decreased the number of vomitings induced by cisplatin in dogs. the antiemetic effect of y-25130 against cisplatin-induced vomiting was more potent than that of metoclopramide and ondansetron, but it showed little differ ... | 1993 | 8107329 |
| subepidermal vesiculobullous filarial dermatitis in free-ranging american badgers (taxidea taxus). | skin and superficial lymph nodes from the 65 juvenile (< 1 year old) and adult free-ranging american badgers (taxidea taxus) of both sexes that were killed from late july to late october 1991 as part of the recovery program for the endangered black-footed ferret (mustela nigripes) were examined for evidence of filaria taxideae. fifty-one badgers (51/64, 80%) were infected. both adult badgers (30/32, 94%) and juvenile badgers (21/32, 67%) were infected by adult filarial worms, which occurred most ... | 1993 | 8212456 |
| efficacy of equine influenza vaccines for protection against a/equine/jilin/89 (h3n8)--a new equine influenza virus. | a new h3n8 equine influenza virus [a/equine/jilin/1/89 (eq/jilin)] appeared in northeastern china in 1989 and caused high mortality in horses; the available evidence indicates that it has not yet spread outside this region of the world. serological analysis with postinfection ferret sera in haemagglutination inhibition (hi) tests confirmed that eq/jilin is antigenically distinct from h3n8 equine influenza viruses isolated between 1963 and 1991 and also showed that a current equine influenza viru ... | 1993 | 8212831 |
| the immunotargeting approach to adjuvant-independent immunization with influenza haemagglutinin. | by conjugating the bromelain fragment of the influenza virus haemagglutinin (bha) to a mouse monoclonal antibody specific for a species-crossreactive, framework determinant on class ii major histocompatibility complex molecules, the adjuvant-independent immunotargeting approach could be compared with more conventional immunizations involving freund's complete adjuvant (fca) and alum, in different species. both rabbits and ferrets made significant virus-blocking igg responses to the immunotargete ... | 1993 | 8212832 |
| plasticity in the gastric inhibitory innervation after immunization against vip and vagotomy in the ferret. | changes in gastric corpus tone have been characterized during two procedures that compromise the major inhibitory innervation of the stomach: immunoneutralization of endogenous vasoactive intestinal polypeptide (vip) and chronic vagotomy. after both procedures, there was a small but significant increase in intracorpus pressure generated during ramp increases in volume compared with sham immunized controls but not when the procedures were combined in vagotomized immunized animals. adaptation in t ... | 1993 | 8214064 |
| benzocaine-induced methemoglobinemia attributed to topical application of the anesthetic in several laboratory animal species. | in a screening study, a common benzocaine-containing anesthetic was topically applied to the following species: dogs (n = 11), domestic shorthair cats (n = 38), long-evans rats (n = 22), sprague-dawley rats (n = 11), ferrets (n = 6), rhesus monkeys (n = 10), cynomolgus monkeys (n = 10), owl monkeys (n = 10), new zealand white rabbits (n = 18), miniature pigs (n = 9), icr mice (n = 4), c3h mice (n = 4), and c57bl/10snj mice (n = 24). all animals, except mice and rats, received a 2-second spray to ... | 1993 | 8214904 |
| bilateral control of the orbicularis oculi muscle by one cerebellar hemisphere in the ferret. | reports that lesions of the anterior interpositus nucleus in the cerebellum or of the cerebellar cortex abolish classically conditioned eyeblink responses were originally taken to indicate that the cerebellum is the locus of learning. this interpretation has recently been questioned by reports that conditioned responses may recover after ipsilateral cerebellar lesions. it cannot be excluded, however, that the recovered responses were produced by the intact contralateral hemisphere. in order to d ... | 1993 | 8219039 |
| interleukin-1 beta modulates myocardial contraction via dexamethasone sensitive production of nitric oxide. | nitric oxide released by a calcium dependent constitutive no synthase in endocardial endothelial cells exerts characteristic effects on myocardial contraction. interleukin 1 beta (il-1) induces the expression of a different calcium independent no synthase in several tissues. activity of the latter enzyme has recently been identified in cardiac myocytes but its functional effects are unknown. the aim of this study was to investigate the effects of il-1 on contraction of isolated ferret papillary ... | 1993 | 8221802 |
| effects of cyclopiazonic acid on ca(2+)-activated tension production in skinned skeletal muscle fibres of the ferret. | the effect of cyclopiazonic acid, an inhibitor of sarcoplasmic reticulum ca(2+)-atpase, was investigated on force generation by the contractile apparatus in triton-skinned fibres from extensor digitorum longus and soleus. concentrations of cyclopiazonic acid lower than 10 microm were without effect on ca(2+)-activated tension in both types of muscles. in contrast, in soleus, cyclopiazonic acid (20, 50, 100 microm) was found to shift reversibly the relation-tension pca (-log[ca2+]) towards lower ... | 1993 | 8223923 |
| histopathologic features and post-surgical sequelae of 57 cutaneous neoplasms in ferrets (mustela putorius furo l.). | the study of the signalment, histomorphologic features, and post-surgical clinical progress of 57 cutaneous neoplasms in 55 domestic ferrets (mustela putorius furo l.) was based on diagnostic pathologic accessions (1987-1992) from 142 ferrets. mean age of the group was 4.3 years; 31/54 (57%) were female and 23/54 (43%) were male. thirty-three (58%) of the cutaneous neoplasms were basal cell tumors. the mean age of ferrets with basal cell tumor was 5.2 years, and 23/33 (70%) were female. histolog ... | 1993 | 8116142 |
| the tachykinin nk1 receptor antagonist cp-99,994 attenuates cisplatin induced emesis in the ferret. | the tachykinin nk1 receptor antagonist cp-99,994 ((+)-(2s,3s)-3-(2-methoxybenzylamino)-2-phenylpiperidine) (0.3-3 mg/kg i.v.), but not its inactive enantiomer cp-100,263, attenuated the retching and vomiting induced by cisplatin (10 mg/kg i.v.) in the ferret. cp-99,994, 3 mg/kg i.v., prevented vomiting in all ferrets tested. since substance p is the preferred ligand at the nk1 receptor subtype these data support a role for the release of this peptide during the emetic response induced by cytotox ... | 1993 | 8119305 |
| modulation of basal l-type ca2+ current by adenosine in ferret isolated right ventricular myocytes. | 1. the whole-cell configuration of the gigaohm seal voltage clamp and an internal perfusion technique were used to study the effects of adenosine on the basal l-type ca2+ current (ica) in enzymatically isolated right ventricular myocytes of ferrets. basal l-type ica was isolated by using a na(+)- and k(+)-free saline (replacement by n-methyl-d-glucamine+, cs+ and tea+, respectively). all experiments were conducted at room temperature (22-24 degrees c). 2. basal ica was markedly reduced during ex ... | 1993 | 8120807 |
| modulation of l-type ca2+ current by extracellular atp in ferret isolated right ventricular myocytes. | 1. the effects of extracellular adenosine triphosphate (atp) on the basal l-type ca2+ current (ica) were investigated in ferret isolated right ventricular myocytes using the gigaohm seal voltage clamp in the whole-cell and cell-attached configurations. 2. micromolar levels of extracellular atp reversibly inhibited ica in a concentration-dependent manner, without any significant changes in the voltage dependence of either the peak ica i-v relationship or steady-state activation curve. 3. in contr ... | 1993 | 8120808 |
| negative lusitropy and abnormal calcium handling in hypoxic cardiac myocytes exposed to the calcium-sensitizer emd 53998. | positive inotropic agents that increase the sensitivity of myofilaments to calcium have recently been described (kitada et al., 1987; cottney et al., 1990; ferroni et al., 1991; lee and allen, 1991; beier et al., 1992). these drugs appear to augment contractility independently of camp or calcium, and thus may have fewer of the adverse side effects seen with other currently available agents (katz, 1986; packer 1989). the clinical utility of "calcium-sensitizers" has been questioned on the theoret ... | 1993 | 8230239 |
| enteric coccidiosis in a ferret. | 1993 | 8231097 | |
| host-specific hemagglutination of influenza a (h1n1) virus. | h1n1 strains of influenza a virus isolated during the influenza season of 1991-92 were divided into two groups according to the property of host-specific hemagglutination. group 1 viruses agglutinated human and chicken red blood cells. group 2 viruses agglutinated human but not chicken red blood cells. the viruses of both groups, however, showed the same antigenic structure determined with ferret antisera. the virus clones which were plaque-purified twice from a group 2 virus retained the charac ... | 1993 | 8246828 |
| tgf-alpha protein and receptor localization in laryngotracheal tissue. | both normal cell turnover and healing of laryngeal and tracheal injuries involve cell migration and mitosis. the proteins that regulate normal cell turnover and wound healing in the larynx and trachea have not been established. it is possible that peptide growth factors, such as transforming growth factor-alpha (tgf alpha) acting through its receptor (egf/tgf alpha-r), participate in the regulation of these processes. to investigate this hypothesis, we analyzed laryngotracheal cells for tgf alph ... | 1993 | 8247573 |
| development of orientation selectivity in ferret visual cortex and effects of deprivation. | the orientation selectivity of cells in ferret primary visual cortex was studied during normal development and in animals deprived of vision or of visual cortical activity. in normal animals from the age when visual responses were first recorded (postnatal day 23) through postnatal week 5, only about 25% of cells showed orientation-selective responses. by postnatal week 7, cortical responses had matured to an adult-like state, with approximately 75% of cells clearly selective for orientation. th ... | 1993 | 8254372 |
| developmental changes produced in the retinofugal pathways of rats and ferrets by early monocular enucleations: the effects of age and the differences between normal and albino animals. | early monocular enucleations were done in rats, either at embryonic day 16 (e16) or on the day of birth, and the surviving uncrossed pathway was studied either at birth for some of the animals enucleated prenatally, or in the adult for all of the other animals. the uncrossed pathways were studied by using hrp as a retrograde tracer. the neonatal enucleations showed the increase of the surviving uncrossed component previously documented by others. in contrast to this, a prenatal enucleation produ ... | 1993 | 8254374 |
| sex and regional differences in intracellular localization of estrogen receptor immunoreactivity in adult ferret forebrain. | estrogen receptors were visualized in adult ferret brains using the h222 estrogen receptor antibody and immunocytochemical techniques. h222 immunoreactive (h222ir) cell nuclei were present in many forebrain regions in gonadectomized ferrets of both sexes. in many instances, h222ir cells also had immunoreaction product in their processes. all cells with h222ir processes also contained h222ir nuclei. more h222ir processes were observed in females in the medial and lateral preoptic area/anterior hy ... | 1993 | 8255392 |
| antineoplastic activity and tolerability of a novel heterocyclic alkylphospholipid, d-20133. | octadecyl-[2-(n-methylpiperidinio)ethyl]-phosphate (ompep, d-20133), a heterocyclic analogue of hexadecylphosphocholine (mil), has been synthesized in an attempt to increase the therapeutic range of the parent compound. the antineoplastic activity of the novel alkylphospholipid was compared with that of mil in dimethylbenz(a)anthracene-induced mammary carcinoma of the rat. using tumors of different sizes and repeated daily doses as well as high single doses, we achieved marked remissions with ei ... | 1993 | 8258191 |
| immune responses and chronic lymphedema in experimental filariasis. | chronic lymphedema is a clinically important manifestation of lymphatic filariasis but the factors which govern the development and progression of lymphedema remain unclear. because immune responses are major determinants of disease expression in filariasis, we compared immune responses in ferrets reinfected with b. malayi in which disease expression varied from virtually no overt disease to severe chronic lymphedema of the infected limbs. no immune correlates specific for development of chronic ... | 1993 | 8258985 |
| functional consequences of neonatal unilateral cochlear removal. | the physiological consequences of unilateral cochlear removal in infancy were assessed by recording the responses of neurones in the ferret inferior colliculus and superior colliculus to acoustic stimulation of the intact ear. animals were lesioned between postnatal days p5 and p40 and survived for at least a year prior to recording. in the inferior colliculus ipsilateral to the intact ear, neurones had lower thresholds and wider dynamic ranges following earlier (p5) than following later (p40, a ... | 1993 | 8234738 |
| myocardial salvage efficacy of a thromboxane receptor antagonist, sq, 30,741, in relation to inhibition ex vivo of platelet function in the ferret. | the myocardial salvage efficacy of a thromboxane a2/prostaglandin endoperoxide (tp) receptor antagonist has not been previously determined in a ferret model of ischemia and reperfusion. assessments of the reproducibility of infarct size resulting from a 90 min period of occlusion followed by 5 hr of reperfusion of the left anterior descending coronary artery in saline-treated control ferrets revealed a consistent mean level of tissue damage representing 23.1 +/- 1.4% of the left ventricle. in su ... | 1993 | 8234831 |
| aleutian disease in the ferret. | 1993 | 8236711 | |
| development of the photoreceptor outer segment-like cilia of the csf-contacting pinealocytes of the ferret (putorius furo). | the postnatal development of the pineal organ of the ferret (putorius furo) was investigated electron-microscopically with special interest given to the cerebrospinal fluid (csf)-contacting pinealocytes and their large, vesiculated cilia. in the pineal of the newborn ferrets, there is a lumen--a pineal ventricle--which is a diverticle of the third ventricle of the diencephalon. the luminal surface of the pineal is bordered by ependymal cells and csf-contacting pinealocytes. a sensory, 9 x 2 + 0 ... | 1993 | 8129982 |
| mechanism of the direct, negative inotropic effect of etomidate in isolated ferret ventricular myocardium. | etomidate exerts a mild, positive inotropic effect in rat ventricular myocardium, yet has a negative inotropic effect in isolated rabbit ventricular myocardium. the aim of this study was to investigate the mechanisms of etomidate's inotropic effect and its underlying mechanism in isolated ferret ventricular myocardium (which shows similar physiologic characteristics as human ventricular myocardium) and in frog ventricular myocardium, in which ca++ ions for myofibrillar activation are derived alm ... | 1993 | 8267205 |
| pharmacological effects of the new gastroprokinetic agent mosapride citrate and its metabolites in experimental animals. | the pharmacological effects of the metabolites m1 and m2 of mosapride citrate ((+/-)-4-amino-5-chloro-2-ethoxy-n-[[4-(4-fluorobenzyl)-2-morpholi nyl] methyl]benzamide citrate, as-4370, cas 112885-42-4), a gastroprokinetic agent with serotonin 5-ht4 receptor agonist property, were compared with those of mosapride. in isolated guinea-pig ileum treated with phenoxybenzamine, the metabolites m1 and m2 enhanced electrically-evoked contractions with ec50 values of 1.2 x 10(-7) mol/l and 1.0 x 10(-6) m ... | 1993 | 8267674 |
| spike trains and signaling modes of neurons in the ferret lateral geniculate nucleus. | intracellular recordings were used to examine the action potential firing modes of cells in the ferret lateral geniculate nucleus (lgn) in vitro. we compared the effects of altered membrane voltage on patterns of action potential trains evoked by direct current injection and by retinal afferent (synaptic) stimulation. the results confirm that lgn cells in the ferret can fire action potentials in the "burst" and "tonic" modes that have been described previously for other species. at depolarized m ... | 1993 | 8270022 |
| relationship between intracellular ph and metabolite concentrations during metabolic inhibition in isolated ferret heart. | 1. intracellular ph (phi) and phosphorus metabolites were measured in isolated ferret hearts with 31p nuclear magnetic resonance (nmr). 2. the application of cyanide (to mimic hypoxia) produced a fall of the concentration of phosphocreatine ([pcr]) and a rise of those of inorganic phosphate ([pi]) and sugar phosphates. these were accompanied by an intracellular acidosis. 3. if glycolysis was partly inhibited by prior exposure to a glucose-free solution then the application of cyanide also produc ... | 1993 | 8145137 |
| fluorocarbon simulation of myocardial ischaemia and reperfusion: studies of relationships between force and intracellular calcium. | the aim was to compare the effects of simulated ischaemia-reperfusion with those of hypoxia-reoxygenation in isolated muscle preparations from the ferret right ventricle. | 1993 | 8275536 |
| visual projections induced into the auditory pathway of ferrets: ii. corticocortical connections of primary auditory cortex. | although the development of corticocortical projections has been well studied, less is known about the role of sensory inputs in the specification of these connections. as part of an ongoing series of studies in our laboratory, we have examined the role of thalamic input modality in the development of corticocortical connections. these studies involve making unilateral lesions and inducing retinal inputs into the auditory thalamus (mgn) during early development in ferrets, thereby conferring vis ... | 1993 | 8277005 |
| light- and electron-microscopic immunocytochemistry of somatotropes in the anterior pituitary gland of european ferret, mustela putorius furo. | light-microscopic immunocytochemistry of ferret anterior pituitary revealed the localization of somatotropes in the pars distalis, but no immunoreactive cells were detected in the pars tuberalis. ultrastructural studies by superimposition immunocytochemistry and immuno-electron microscopy, elucidated the morphological heterogeneity of these somatotropic cells. they were classified into 2 subtypes on the basis of size of the secretory granules. type-i cells with small granules (mean diameter, 192 ... | 1993 | 8402825 |
| mnng-induced gastric carcinoma in ferrets infected with helicobacter mustelae. | n-methyl-n-nitro-n'-nitrosoguanidine (mnng) is a gastric carcinogen in several animal species and has been used in a number of systems to dissect the co-carcinogenic potential of various compounds in the induction of gastric adenocarcinoma. recent epidemiological evidence suggests that helicobacter pylori may play a role as a co-carcinogen in the etiology of this tumor in humans and we have been interested in developing an animal model to study this possibility. a related organism, h. mustelae, ... | 1993 | 8403224 |
| characterisation of the peptido-leukotriene receptor pl2 on the ferret spleen strip. | the peptido-leukotriene receptor(s) (pl) on the ferret isolated spleen strip have been characterised by functional studies using the naturally occurring leukotrienes (lts), a range of structurally distinct pl antagonists, and by ligand binding studies. ltb4 (0.01-10 microm) was inactive on ferret spleen whereas ltc4, ltd4 and lte4 produced concentration-related contractions with maximal responses, relative to noradrenaline, of 57% (ec50 0.28 microm), 60% (ec50 0.5 microm) and 7% respectively. th ... | 1993 | 8405079 |
| kinetic analysis of the l-type calcium current in enzymatically dissociated ferret ventricular myocytes. | the l-type calcium current (ica-l) was studied in single ferret ventricular myocytes using whole-cell recording with single patch pipettes. voltage-clamp experiments were performed at room temperature with internal and external na(+)- and k(+)-free tyrode solutions in order to isolate ica-l. for depolarizing steps eliciting small ica-l the decay of the current is best described by one exponential. for depolarizing steps eliciting large ica-l (i.e. between -10 and +30 mv), the decay of the curren ... | 1993 | 8405909 |
| genetic stability and diversity of pneumocystis carinii infecting rat colonies. | there is increasing molecular and antigenic evidence that pneumocystis carinii organisms isolated from humans, ferrets, and rats are different species. in contrast, little is known about the extent of genetic diversity among p. carinii strains found within a single mammalian species. in the present study, electrophoretic karyotypes were obtained from p. carinii prepared from 10 chronically immunosuppressed rat colonies to investigate diversity at the chromosomal level. most organism preparations ... | 1993 | 8406881 |
| the re-establishment of the representation of the dorso-ventral retinal axis in the chiasmatic region of the ferret. | this study has examined the representation of the dorso-ventral retinal axis in the optic nerve and tract of the ferret, as well as the associated fiber transformations which take place within the chiasmatic region. in one series of experiments, dorsal or ventral retinal lesions were made to induce fiber degeneration along the pathway, from which semi-thin sections were then stained for degenerating myelin. in a second series, implants of the carbocyanine dye, dii, were made into the caudo-media ... | 1993 | 8217945 |
| hyperadrenocorticism associated with adrenocortical tumor or nodular hyperplasia of the adrenal gland in ferrets: 50 cases (1987-1991). | adrenocortical adenoma, nodular hyperplasia, or carcinoma was diagnosed in 50 ferrets. thirty-five (70%) ferrets were female and 15 (30%) were male. the mean age at which clinical signs were first noticed was 3.4 years (range, 1 to 7 years). clinical signs included large vulva (n = 31; 89% of females), alopecia (n = 43; 86%), pruritus (n = 20; 40%), and increased consumption of water and increased urine output (n = 4; 8%). a mass was palpated at the cranial pole of the kidney during physical exa ... | 1993 | 8407489 |
| the effects of different antiemetic agents on morphine-induced emesis in ferrets. | there is interest in the development of antiemetics other than dopamine receptor antagonists for the treatment of postoperative nausea and vomiting. a ferret model of morphine-induced emesis may have wider application in evaluating newer agents than the apomorphine dog model. this study describes the conditions for morphine-induced emesis in ferrets and evaluates five antiemetics that are prototypical of three different mechanisms. the average numbers of vomiting and retching episodes induced by ... | 1993 | 8223924 |
| 2-(quinuclidin-3-yl)pyrido[4,3-b]indol-1-ones and isoquinolin-1-ones. potent conformationally restricted 5-ht3 receptor antagonists. | several series of n-(quinuclidin-3-yl)aryl and heteroaryl-fused pyridones were synthesized and evaluated for 5-ht3 receptor affinity. in the heteroaryl series, 2-(quinuclidin-3-yl)tetrahydropyrido-[4,3-b]indol-1-one (8a) and the 4,5-alkano-bridged analogues (14 and 15) displayed high 5-ht3 receptor affinity with pki values > 9. the (3s)-quinuclidinyl isomers had > 10 fold higher affinity than the (3r)-isomers. in a series of 2-quinuclidin-3-yl)isoquinolin-1-ones, derivatives substituted with sma ... | 1993 | 8410977 |
| resinferatoxin, an ultrapotent capsaicin analogue, has anti-emetic properties in the ferret. | resinferatoxin (100 micrograms/kg, s.c.), the ultrapotent analogue of capsaicin, when given acutely blocked radiation-(200 rads) and copper sulphate (40 mg% 30 ml, p.o.)-induced emesis in ferrets and substantially decreased loperamide (0.5 mg/kg, s.c.)-induced vomiting, without significantly affecting the von bezold-jarisch reflex or gag reflex. it also produced a decrease in core temperature as has been reported for capsaicin. the observation that resinferatoxin reduced or blocked emesis induce ... | 1993 | 8413843 |
| effects of oxygen tension and glucose concentration on ischemic injury in ventilated ferret lungs. | in the ventilated ischemic lung, oxygen tension will increase at a time when glucose depletion may impair antioxidant defenses, thereby predisposing the lung to injury mediated by oxygen radicals. in the unventilated ischemic lung, however, glucose depletion in the setting of low oxygen tension may decrease production of atp, leading to injury by a different mechanism. in this study, we evaluated the role of both oxygen tension and glucose concentration on ischemic injury in isolated ferret lung ... | 1993 | 8226535 |
| effects of 5-hydroxytryptamine on discharge of vagal mucosal afferent fibres from the upper gastrointestinal tract of the ferret. | this study was undertaken to determine the effects of 5-hydroxytryptamine (5-ht) on vagal mucosal afferent endings and how this may relate to their sensitivity to other stimuli. single afferent fibres with receptive fields in the mucosa of the upper gastrointestinal tract were recorded from the cervical vagus of urethane anaesthetized ferrets. the selection criteria included failure to respond to luminal distension (i.e., vagal tension receptors were excluded). all fibres tested responded to muc ... | 1993 | 8227963 |
| effects of 5-hydroxytryptamine (5-ht) on the discharge of vagal mechanoreceptors and motility in the upper gastrointestinal tract of the ferret. | this study was undertaken to investigate the sensitivity of vagal mechanoreceptor afferent fibres from the stomach and small intestine to 5-hydroxytryptamine (5-ht, 10 and 50 micrograms), and to relate this to motor activity. the discharge of single afferent fibres was recorded from the cervical vagus of urethane anaesthetized ferrets. these had receptive fields in the corpus (15 fibres), antrum (11 fibres), duodenum (3 fibres) or jejunum (1 fibre), and their discharge was excited by distension ... | 1993 | 8227964 |
| cell migration in cultured cerebral cortical slices. | in order to investigate the cellular mechanisms of migration and lamination in the mammalian cerebral cortex, we have cultured living slices of the developing telencephalon and tracked the migration of newly generated cortical neurons. slice cultures made from neonatal ferret cortex were maintained in roller tubes and survived well for several weeks in vitro. cells generated on postnatal day (p) 0 or 1 were labeled with the thymidine analog 5-bromo-2-deoxy-uridine (brdu), and their movements wer ... | 1993 | 8432395 |
| pronounced direct inhibitory action mediated by adenosine a1 receptor and pertussis toxin-sensitive g protein on the ferret ventricular contraction. | an adenosine a1 receptor agonist r-n6-phenylisopropyladenosine (r-pia) elicited a pronounced negative inotropic effect with the ec50 value of 0.69 mumol/l in the presence of a beta-adrenoceptor blocking agent bupranolol (0.3 mumol/l) in the isolated ferret papillary muscle. the negative inotropic effect of r-pia was not associated with changes in cyclic amp level. adenosine and other a1 receptor agonists also elicited a negative inotropic effect. dpcpx (1,3-dipropyl-8-cyclopentyl xanthine) antag ... | 1993 | 8232606 |
| relative roles of intracellular ca2+ and ph in shaping myocardial contractile response to acute respiratory alkalosis. | during acute respiratory alkalosis, myocardial contractility initially increases but then declines toward control levels. to elucidate the mechanism of this response, two parallel strategies were adopted: isovolumic left ventricular developed pressure (dp) and intracellular ph (phi) were measured in isolated ferret hearts using 31p-nuclear magnetic resonance spectroscopy, and isometric developed tension (dt) and intracellular ca2+ concentration ([ca2+]i) were measured in ferret papillary muscles ... | 1993 | 8238582 |
| eustachian tube function in the ferret. | the role of viral upper respiratory tract infections (uri) in the pathogenesis of otitis media (om) may be related to eustachian tube (et) dysfunction. preliminary experimental evidence suggests that the ferret may be an appropriate animal for modeling of pathophysiologic process related to uri's and et dysfunction. in an effort to determine the applicability of this animal model, normal et function was evaluated in 10 ferrets using the inflation-deflation and forced-response testing protocols. ... | 1993 | 8442426 |
| crohn's disease and anastomotic recurrence: microvascular ischaemia and anastomotic healing in an animal model. | microvascular injury and ischaemia may be factors in anastomotic recurrence after resection for crohn's disease. this hypothesis was explored in a ferret model of multifocal intestinal infarction. at laparotomy, isolated loops of small intestine were injected intraarterially with styrene microspheres (test loop) or saline (control). at a second laparotomy 72 h later, test and control loops were divided and an end-to-end anastomosis performed between test loops (n = 2), test and control loops (n ... | 1993 | 8443664 |
| transient period of correlated bursting activity during development of the mammalian retina. | the refinement of early connections in the visual pathway requires electrical activity in the retina before the onset of vision. using a multielectrode array, we have shown that the spontaneous activity of cells in the neonatal ferret retina is correlated by patterns of periodically generated traveling waves. here, we examine developmental changes in the characteristics of the waves and show that retinal ganglion cells participate in these patterns of activity, which are seen during the same per ... | 1993 | 8240814 |
| gastric bacteria other than helicobacter pylori. | since the culture of helicobacter pylori from the human stomach in 1983 there has been renewed interest in other bacteria that had been observed in animal stomachs as early as late in the nineteenth century. many of these bacteria have now been isolated and have been shown to belong to the same genus, helicobacter, which currently contains nine species. study of the gastric helicobacters is important because it provides relevant information about how bacteria can survive in the gastric environme ... | 1993 | 8449568 |
| alterations in intracellular calcium and tension of activated ferret papillary muscle in response to step length changes. | 1. to study the effects of mechanical constraints on the calcium (ca2+) affinity of cardiac troponin c, we analysed the tension and aequorin light (al, intracellular ca2+) transients in response to a step length change in aequorin-injected ferret right ventricular papillary muscles. the muscle preparations were continuously activated with ouabain (10(-4) m) (ouabain contracture) or with high frequency stimuli in the presence of ryanodine (5 microm) (tetanic contraction). 2. the tension transient ... | 1993 | 8246184 |
| pneumocystis carinii organisms obtained from rats, ferrets, and mice are antigenically different. | pneumocystis carinii infections were developed in animals immunosuppressed by dexamethasone treatment either from activation of latent infection (ferret) or by transtracheal inoculation with p. carinii-infected lung tissue from the homologous species (rat or mouse). convalescent-phase antisera were obtained by stopping dexamethasone treatment after 2 to 4 weeks and allowing animals 5 to 8 weeks for recovery. p. carinii harvests from infected lungs were purified by differential filtration, solubi ... | 1993 | 8454333 |
| transient cortical pathways in the pyramidal tract of the neonatal ferret. | anterograde transport of wheat germ agglutinin conjugated to horseradish peroxidase (wga-hrp) was used to study transient axons from the visual cortex in the pyramidal tract. injections at birth restricted to the visual cortex labeled axons in the vicinity of the pontine nuclei. two to eight days after birth, axons from the occipital cortex were found posterior to the pontine nucleus, their caudalmost stable target. transient corticospinal axons from the presumptive primary visual cortex did not ... | 1993 | 8308167 |
| short time-scale lv systolic dynamics: pressure vs. volume clamps and effect of activation. | we recently proposed a new model-based approach to quantifying short time-scale left ventricular (lv) systolic dynamics. in this study we examine the hypothesis that the quantitation of lv dynamics using the proposed approach is independent of external mechanical perturbations and the level of activation. mechanical perturbation independence was assessed in seven isolated ferret hearts in which controlled changes in pressure (pressure clamp) or volume (volume clamp) were introduced at the time o ... | 1993 | 8456995 |
| role of ovarian steroids in development of uterine binding sites for prolactin in the ferret. | the objectives of this study were to investigate 1) the presence of specific prolactin (prl) binding sites in the ferret uterus, 2) the uterine location of 125i-labeled ovine prl (oprl) binding sites, 3) changes in uterine prl binding sites during pseudo-pregnancy, and 4) regulation of prl binding sites by ovarian steroids. binding was determined through use of 125i-oprl and 300-800 micrograms of protein from the 50,000 x g particulate fraction. optimal binding occurred within 6 h at 25 degrees ... | 1993 | 8318580 |
| intestinal perfusion of beta-carotene in the ferret raises retinoic acid level in portal blood. | to determine whether beta-carotene (beta-c) can serve as a source of intestinally-derived retinoic acid (ra), either 15,15'-[14c]beta-c or unlabeled beta-c was perfused through 30 cm jejunal segments of ferrets in vivo. portal vein blood was sampled periodically via an indwelling catheter. ra was identified in portal blood by comparing retention times in hplc, by uv absorption, and by derivatization (methylation) and subsequent gc-ms analysis. the ra concentration in the portal blood increased 3 ... | 1993 | 8466944 |
| retinoic acid regulates retinol metabolism via feedback inhibition of retinol oxidation and stimulation of retinol esterification in ferret liver. | when the plasma concentration of retinoic acid is increased, there is an accompanying reduction of circulating levels of retinol, suggesting that retinoic acid may have a regulatory effect on retinol metabolism in vivo. to determine which specific step(s) of retinol metabolism might be regulated by retinoic acid, retinol was incubated with ferret liver microsomes or cytosol with retinoic acid in vitro. incubating the microsomal fraction with retinoic acid resulted in a dose-dependent (up to 0.5 ... | 1993 | 8320566 |
| stimulation of gastrointestinal motility by cisplatin in the ferret: activation of an intrinsic cholinergic mechanism dissociated from emesis. | motility was recorded from the corpus, antrum and small intestine of the urethane anaesthetised ferret. the gastrointestinal effects of the highly emetic cytotoxic anticancer agent, cisplatin were investigated following intravenous administration (10 mg/kg i.v.). following injection, cisplatin induced a prompt onset (< 2 min) increase in motility (tone and contraction amplitude) in all regions with a duration of < 15 min. acute vagotomy did not abolish the effect but reduced the peak amplitude i ... | 1993 | 8321326 |
| in situ hybridization analysis of developmental stages of pneumocystis carinii that are transcriptionally active for a major surface glycoprotein gene. | an abundant glycoprotein on the surface of pneumocystis carinii, termed gpa or gp120, is thought to play a role in the interaction of this opportunistic pathogen with its host. using rna:rna hybridization techniques, the in situ expression of gpa mrna in developmental forms of the organism was investigated in a ferret model of p. carinii pneumonia. the results suggested that the relative abundance of gpa-specific mrna was variable in different developmental stages of ferret p. carinii. p. carini ... | 1993 | 8469111 |
| distribution of calcitonin gene-related peptide immunoreactive nerve fibers in the human submandibular gland. | the indirect immunofluorescence technique was used to study the distribution of calcitonin gene-related peptide (cgrp) in human submandibular gland. a relatively low number of thin varicose fibers with intense immunofluorescence for cgrp was seen in samples from seven glands. these cgrp-immunoreactive (cgrp-ir) nerve fibers were mainly seen around or in close contact with intra- and interlobular blood vessels. some cgrp-ir nerve fibers were also found in association with intra- and interlobular ... | 1993 | 8469411 |
| predicting prognosis of gastrointestinal smooth muscle tumors. role of clinical and histologic evaluation, flow cytometry, and image cytometry. | both flow cytometry (fcm) and morphometry have been proposed as techniques for predicting the prognosis of gastrointestinal (gi) smooth muscle tumors (smts). in particular, dna aneuploidy by fcm has been associated with high histologic grade and shortened survival, whereas the dna index determined by image cytometry has been proposed as a criterion for the diagnosis of malignancy. to further define the potential roles of these two techniques, we performed a variety of morphometric and fcm measur ... | 1993 | 8333557 |
| the regulation of intracellular mg2+ in guinea-pig heart, studied with mg(2+)-selective microelectrodes and fluorochromes. | because of the reported presence of a na(+)-mg2+ exchanger in guinea-pig but not in ferret myocardium, the mg2+ extrusion mechanism in guinea-pig myocardium has been reinvestigated using mg(2+)- and na(+)- selective microelectrodes and the fluorochromes mag-fura-2 and -5. the mean [mg2+]i measured with microelectrodes in trabeculae or papillary muscles was 0.72 mmol/l (n = 22, thirteen experiments; range 0.42-1.23 mmol/l). increasing [mg2+]o from 0.5 mmol/l to either 10.5 or 20 mmol/l caused sma ... | 1993 | 8471242 |
| spontaneous neoplasia in the ferret (mustela putorius furo). | thirteen spontaneous tumours in ferrets from two laboratory breeding colonies are described, including two types not previously reported in this species, namely, uterine teratoma and neurilemmoma. the literature on tumours in laboratory and domestic ferrets is comprehensively reviewed from the first reported case in 1950. only 20 cases were reported from 1950 to 1979, nearly all from laboratory or zoological collections. in the following 10 years more than 170 further cases were reported, about ... | 1993 | 8473565 |
| recurrent mycobacterium bovis infection following a ferret bite. | 1993 | 8473774 | |
| studies directed toward the design of orally active renin inhibitors. 2. development of the efficacious, bioavailable renin inhibitor (2s)-2-benzyl-3- [[(1-methylpiperazin-4-yl)sulfonyl]propionyl]-3-thiazol-4-yl-l-alanine amide of (2s,3r,4s)-2-amino-1-cyclohexyl-3,4-dihydroxy-6-methylheptane (a-72517). | employing a set of empirical guidelines for the design of well-absorbed renin inhibitors, we have followed two strategies to improve potency while maintaining bioavailability. one process involved incorporation of an extended n-terminal residue bearing a weakly basic substituent and is exemplified by compound 25. the other approach centered on the inclusion of an n-terminal sulfonamide and culminated in the discovery of inhibitor 32 (a-72517). both 25 and 32 showed excellent bioavailability in t ... | 1993 | 8474102 |
| the protective effect of 4-hydroxy-2-methyl-n-[2-(tetrazol-5-yl)- phenyl]-2h-1, 2-benzothiazine-3-carboxamide-1, 1-dioxide monosodium salt (hx-1920) on cisplatin-induced toxicity in rats. | the protective effect of 4-hydroxy-2-methyl-n-[2-(tetrazol-5-yl)-phenyl]-2h-1, 2-benzothiazine-3-carboxamide-1, 1-dioxide monosodium salt (hx-1920) on the nephrotoxicity of cisplatin was studied in rats. effects of hx-1920 on antitumor activity and emesis induced by cisplatin were also examined using mice and ferrets, respectively. all 10 rats injected with both hx-1920 and ld50 of cisplatin survived for 14 days. after 24 hr, co-administration of hx-1920 significantly increased the urinary excre ... | 1993 | 8474148 |
| cellular mechanism of the positive inotropic effect of hydralazine in mammalian myocardium. | 1. the purpose of this study was to elucidate the cellular mechanism of the positive inotropic effect of hydralazine, a vasodilator widely used for afterload reduction in patients with heart failure that has also been reported to have positive inotropic effects on the heart. after isolation, right ventricular papillary muscles from the ferret were maintained in bicarbonate-buffered salt solution (30 degrees c). a concentration-response relationship was obtained for hydralazine (10(-6) to 10(-3) ... | 1993 | 8358564 |
| organization of response areas in ferret primary auditory cortex. | 1. we studied the topographic organization of the response areas obtained from single- and multiunit recordings along the isofrequency planes of the primary auditory cortex in the barbiturate-anesthetized ferret. 2. using a two-tone stimulus, we determined the excitatory and inhibitory portions of the response areas and then parameterized them in terms of an asymmetry index. the index measures the balance of excitatory and inhibitory influences around the best frequency (bf). 3. the sensitivity ... | 1993 | 8459273 |
| synaptophysin immunohistochemistry reveals inside-out pattern of early synaptogenesis in ferret cerebral cortex. | synaptogenesis in the ferret cerebral cortex was examined from the day of birth to adulthood with an antibody against synaptophysin at the light and electron microscopic levels. due to the premature birth of ferrets, the generation of cells destined to the upper cortical layers and their subsequent migration to their final positions in the cortical plate are largely postnatal events. throughout the newborn ferret cerebral cortex, a high amount of synaptophysin immunoreactivity was present within ... | 1993 | 8468403 |
| neural changes in periapical lesions after systemic steroids in the ferret. | this study was intended to clarify the relationship between the neural changes which occur around the apex of the ferret canine after pulpectomy and the inflammatory process induced by the procedure. in 12 young adult ferrets, under general anesthesia, the pulps in the mandibular canine teeth were removed and replaced with gutta percha and grossman's sealer. six of the animals were treated with dexamethasone to reduce the inflammatory response. three months later, the animals, again under genera ... | 1993 | 8496482 |
| cardiac electrophysiologic and antiarrhythmic actions of 3,4-dihydro-1'-[2-(benzofurazan-5-yl) ethyl]-6-methanesulfonamidospiro [(2h)-1-benzopyran-2,4'-piperidin]-4-one hcl (l-691,121), a novel class iii agent. | the cardiac electrophysiologic and antiarrhythmic actions of 3,4-dihydro-1'-[2-(benzofurazan-5-yl)ethyl]-6-methyl-sulfonamid ospiro [(2h)-1-benzopyran-2,4'-piperidin]-4-one hcl (l-691,121), a novel spirobenzopyran piperidine class iii agent, were assessed in vitro and in vivo. in ferret isolated papillary muscles, l-691,121 significantly prolonged effective refractory period (ec25 = 13 nm) and elicited a modest positive inotropic effect. in guinea pig isolated ventricular myocytes, l-691,121 pro ... | 1993 | 8496818 |
| chiasmatic course of temporal retinal axons in the developing ferret. | recent studies on the distribution of optic axons in the mature visual pathways, as well as on the genesis of their ganglion cells of origin, suggest that the time of axonal arrival at the optic chiasm determines the side of the brain to which a temporal retinal axon will project. the present study has examined this issue directly in fetal ferrets, by determining the projection of the temporal retina at different developmental stages. fetuses of known gestational age were fixed with paraformalde ... | 1993 | 8468406 |
| helicobacter mustelae and helicobacter pylori bind to common lipid receptors in vitro. | helicobacter pylori is a recently recognized human pathogen causing chronic-active gastritis in association with duodenal ulcers and gastric cancer. helicobacter mustelae is a closely related bacterium with similar biochemical and morphologic characteristics. h. mustelae infection of antral and fundic mucosa in adult ferrets causes chronic gastritis. an essential virulence property of both helicobacter species is bacterial adhesion to mucosal surfaces. the aim of this study was to determine whet ... | 1993 | 8500901 |
| cloning and genetic characterization of the helicobacter pylori and helicobacter mustelae flab flagellin genes and construction of h. pylori flaa- and flab-negative mutants by electroporation-mediated allelic exchange. | helicobacter pylori is one of the most common human pathogens. it causes chronic gastritis and is involved in the pathogenesis of gastroduodenal ulcer disease and possibly gastric carcinoma. helicobacter mustelae is a bacterium closely related to h. pylori that causes gastritis and ulcer disease in ferrets and is therefore considered an important animal model of gastric helicobacter infections. motility, even in a viscous environment, is conferred to the bacteria by several sheathed flagella and ... | 1993 | 8501031 |
| sarcoplasmic reticulum calcium mobilization in right ventricular pressure-overload hypertrophy in the ferret: relationships to diastolic dysfunction and a negative treppe. | in a model of right-ventricular pressure-overload hypertrophy (poh) in the ferret, action potential duration (to 90% repolarization) was found to be significantly longer (228 +/- 11 vs 314 +/- 12 ms) with no change in amplitude (85 +/- 3 vs 85 +/- 2 mv) or resting membrane potential (-79 +/- 1.5 vs -79 +/- 1 mv) for control and poh, respectively. peak sarcoplasmic reticulum ca2+ release (expressed as the logarithm of the fractional luminescence, -4.2 +/- 0.1 vs -4.4 +/- 0.3) and resting calcium ... | 1993 | 8469611 |
| factors influencing measurement of protein reflection coefficient by filtered volume technique. | in isolated perfused organs, the protein reflection coefficient (sigma) can be estimated by comparing increases in hematocrit (hct) and protein concentration (cp) during transvascular fluid filtration. in this study, we developed an equation for sigma to examine the potential influences of perfusate leak, evaporation, and hemolysis-induced changes in red blood cell volume and perfusate water. we also performed experiments in isolated ferret lungs to quantitate the magnitude of these potential so ... | 1993 | 8482680 |