Publications

[epidemiological studies on protozoan infection in gyeong-gi do and jeonra bug do]	the present study was designed to elucidate the recent prevalence of the intestinal protozoa and to demonstrate the relationship on prevalence of the surveyed population with their academic career and living conditions. during the period from may to july 1981, total 2,735 fecal specimens were collected from the inhabitants of 11 areas in gyeong-gi do (province) near by seoul city, and 6 areas in jeonra bug do, southwestern part of korea. the samples were fixed with saf solution and were examined ...	1984	12891041
cellulose acetate precipitation test in sero-diagnosis of amoebiasis.	cellulose acetate precipitation (cap) test for the detection of antibody against entamoeba histolytica using the axenic antigen, was performed on the 127 serum samples obtained from patients with amoebic liver abscess (14), amoebic hepatitis (21), amoebic dysentry (11), amoebic colitis (31), other parasitic infestations (25) and normal individuals (25). the percent positivity was 100, 95.23, 90.9, 67.74 and 16 and 12 respectively whereas the corresponding figures for the indirect immunoflouresce ...	1984	12055791
[electron-microscopic studies on fine structure and enzyme activity in the axenic and conventional strains of entamoeba histolytica]	the metabolism of entamoeba histolytica would be affected by various environmental factors, and alteration of the environment was known to affect the fine structure of e. histolytica. the present study was designed electronmicroscopically to investigate the ultrastructure and enzyme activities in the axenic and conventional strains of e. histolytica. the trophozoites of axenically cultivated hk-9 strain and conventional ys-27 and ys-49 strains of e. histolytica were collected and fixed with 4 pe ...	1985	12888671
[medical problems in refugees from sri lanka (tamil)].	recently many refugees from sri lanka have arrived in europe. the purpose of the present investigation was to analyze the subjective complaints and diagnoses in these refugees. one hundred refugees (97 males, 3 females, age 19 to 42 years) were investigated. the most common reasons for consulting a general internist were cough (23%), general pain in soft tissue and joints (21%), disorders of the gastrointestinal tract (19%) and ear or throat complaints (15%). in 43% of the patients no diagnosis ...	1985	3969544
[digestive manifestations of the acquired immunodeficiency syndrome (aids): study in 26 patients].	we studied the gastrointestinal manifestations in 26 cases of aids. the patients belonged to two different epidemiological groups: the first group included thirteen french homosexual men, the second group included 6 haitians, 6 africans and a pakistanian, none of them admit homosexual activity. the clinical manifestations were: chronic watery diarrhea in 17 cases, bloody diarrhea in 2 cases; loss of weight in the 26 cases; dysphagia in five cases; jaundice in one patient (due to kaposi sarcoma o ...	1985	3996815
cell-mediated immunity in hepatic amoebiasis.	cell-mediated immunity (cmi) was measured by blastic transformation of peripheral blood lymphocytes in 26 patients with amoebic liver abscess (ala) and matched control subjects with no demonstrable clinical amoebiasis. during active disease, the mean mitogenic response, measured by the stimulation index of the patients' lymphocytes to entamoeba histolytica antigen, was increased (mean +/- sd: 25.98 +/- 46.62 compared with 11.27 +/- 21.39), whereas that to phytohaemagglutinin (pha) was reduced (5 ...	1985	4002286
health status of teenage school boys in eastern sudan.	in july, 1981 a health survey of teenage boys was conducted in a medical clinic in the eastern sudan, run by the lalmba association, a non-profit aid organization based in the us. the study population was a group of 231 students ranging in age from 13-18, some of whom board at the school. data consisted of a general health questionnaire, height and weight measurements, and a thorough physical examination including stool sample. average height and weight were both less than western teenagers, ...	1985	4006817
mitogen-like monoclonal anti-actin antibodies.	monoclonal antibodies (igm kappa) have been produced to actin isolated electrophoretically from l cell extracts. these monoclonal anti-actin antibodies bind to intact l cells and modulate dna synthesis and cell proliferation, much like affinity-purified polyclonal rabbit antibody to the same mr 42,000 actin. in addition, monoclonal antibodies specific for actin from entamoeba histolytica also bound to and modulated the growth of l cells. a monoclonal antibody directed against a neuroblastoma sur ...	1985	4008931
histopathology of hepatic amoebiasis in guinea-pigs infected through intracaecal and intramesenteric routes.	the comparative histopathological details of amoebic liver abscess were studied following intracaecal and intramesenteric inoculation of entamoeba histolytica into guinea-pigs. the histological changes of amoebic liver abscess formed via the former route were similar to those seen in man. these consisted of a central necrotic area with few lymphomononuclear cells and e. histolytica trophozoites at the periphery of the lesion. the amoebic liver abscess formed via the intramesenteric route, howeve ...	1985	4035733
a mouse model for entamoeba histolytica infection.	2 strains of entamoeba histolytica, saw 760 and saw 408 (non-invasive zymodeme ix and invasive zymodeme ii respectively, sargeaunt & williams 1979) were administered intra-intestinally to 15 inbred and 3 outbred strains of mouse. all were known to be free of the mouse amoeba e. muris. during the investigation, all animals were maintained in plastic film isolator units, under gnotobiotic conditions. both zymodemes were found to be capable of infecting all 18 strains of mice for varying periods of ...	1985	4068657
[entamoeba histolytica as the cause of urinary tract infection after cystectomy and internal urinary diversion with an ileocecal bladder].		1985	4082329
intestinal parasites in kampuchea, takeo province.	fresh stool samples obtained from 1407 adult patients who sought treatment in takeo province hospital and 332 takeo preschool and school-age children from 3 to 18 years of age were examined for the presence of intestinal parasites using the technique of native preparation and the flotation method of faust with subsequent staining with lugol solution to demonstrate cysts. in hospital patients, a total of 13 protozoan and 9 helminth species were diagnosed. the prevalence of entamoeba histolytica ( ...	1985	4086816
experimental amoebiasis in the guinea-pig: sequential histological abnormalities following intracaecal injection of entamoeba histolytica.	the histological abnormalities of amoebic colitis have been well described but their evolution over a period of time has not been clearly examined. to study this, three- to four-week-old guinea-pigs were inoculated intracaecally with 80,000 to 100,000 entamoeba histolytica trophozoites, left untreated and killed at 3, 5, 8, 11, 14 and 21 days, when the caecum was removed and the tissue sectioned and stained with h & e and pas. although there was considerable variation in the type of histological ...	1985	4095744
[gastrointestinal manifestations of aids].	we studied 26 cases of digestive manifestation in aids. the 26 patients were divided into two different epidemiological groups: 13 homosexual men, constituted the first group; no homosexual patient was in the second group which included 6 haitians, 6 africans and a pakistanian. the clinical manifestation were: a watery chronical diarrhea in 17 cases a bloody diarrhea in 2 cases; a loss of weight in the 26 cases; a dysphagia in five cases; a jaundice in one patient (due to kaposi sarcoma of the a ...	1985	4096504
comparison between the efficacy of a single dose of secnidazole with a 5-day course of tetracycline and clioquinol in the treatment of acute intestinal amoebiasis.	a study was carried out in 80 patients with acute intestinal amoebiasis to compare the efficacy of treatment with a single oral dose of 2 g secnidazole and a 5-day course of 750 mg tetracycline plus 1 g clioquinol per day. patients were allocated at random into one or other treatment group and returned for clinical assessment and microscopic examination of stools for the presence of the trophozoite or haematophagous form of entamoeba histolytica on days 1 to 7, 14, 21 and 28. the results showed ...	1985	2932742
chronic diarrhoea in nigerian children.	to study the etiology of chronic childhood diarrhea among nigerian children, 142 patients, aged 6 months to 5 years, with diarrhea for at least 1 month, were evaluated; the study took place during january-december 1983 at the ahmadu bello university teaching hospital, zaria, northern nigeria. enteropathogenic agents were identified in stools of 90 (63%) patients. giardia lamblia and entamoeba histolytica were most commonly detected, representing 41% and 23%, respectively, of all parasitic path ...	1985	3833911
medical problems of the homosexual adolescent.	physicians treating adolescents should take a complete sexual history, including sexual orientation and practices, to determine whether their patients are homosexually active. lesbians are at very low risk for sexually transmitted diseases, but they do have other health concerns. four general groups of conditions may be encountered in homosexually active men: classical sexually transmitted diseases (gonorrhea, infections with chlamydia trachomatis, syphilis, herpes simplex infections, genital wa ...	1985	3839219
squamous cell carcinoma of the uterine cervix subsequent to amebiasis.	we studied the development of a well-differentiated squamous cell carcinoma of the uterine cervix five years after cervical infection with entamoeba histolytica by documenting the findings of specimens obtained by repeated biopsies in a 69-year-old woman. to our knowledge, this is the first report of a malignant neoplasm of the uterine cervix subsequent to amebiasis. amebiasis superimposed on malignant neoplasms of the female genital tract has been reported before. we raise the question of a pos ...	1985	3840988
utility of culture technique for both faeces and biopsy material for the diagnosis of entamoeba histolytica.		1985	3848408
amoebiasis and its control.	on a global scale, 480 million people were recently estimated to be infected with entamoeba histolytica and some 48 million people suffer from invasive amoebiasis. the latter, in the intestinal and extra-intestinal forms, accounts for 40 000 to 110 000 deaths annually. in view of this considerable mortality and morbidity, more research is urgently needed so that more effective control strategies may be introduced.this update article reviews the magnitude of the problem, clinical and laboratory d ...	1985	3876168
lymphocyte subpopulations transformation studies in an experimental model of intestinal and hepatic amoebiasis.	the lymphocyte subpopulations in peripheral blood and blast transformation were studied on days 0, 3, 7, 11, 15, 20 and 35 after infection in progesterone-treated guinea-pigs which had been experimentally infected with entamoeba histolytica by intracaecal inoculation. in animals with intestinal infection, lowered t cell numbers and blast transformation were seen during the acute phase with a subsequent tendency to recover. in guinea-pigs with hepatic amoebiasis, both these parameters were signif ...	1985	3879396
lethal recognition between entamoeba histolytica and the host tissues.	lethal recognition between entamoeba histolytica and the tissues and defence systems of the host results in a continuous interplay that determines the development of pathological lesions: (i) we have identified several of the steps and mediators utilized by the trophozoites to destroy host cells by contact-mediated cytolysis; (ii) we have established that the alternative complement system represents the main defence available to the host against the invading parasite. the amoebae recognize targe ...	1985	2420045
entamoeba histolytica trophozoites in the lumen and mucus blanket of rat colons studied in vivo.	trophozoites of entamoeba histolytica hm-1 were cultivated axenically in tyi-s medium. the amoebae were then transferred into this medium lacking serum (tyi) and inoculated into in vivo colon loops of adult sprague-dawley rats. the trophozoites were rapidly absorbed by the mucus, and few were found free in the luminal fluid by 1 h. by 4 h, the amoebae began to reappear in the lumen, aggregated in sloughed mucus blanket fragments. the colon was examined histologically and by scanning electron mic ...	1985	2856915
seroepidemiological study of antibody responses to the zymodemes of entamoeba histolytica.	in 303 apparently healthy subjects and 54 patients with confirmed invasive amoebiasis amoebae were isolated where possible, and entamoeba histolytica was classified into its various zymodems. 20% of the healthy subjects were antibody-positive; 17 healthy subjects proved to be symptomless carriers of pathogenic zymodemes and, like patients with invasive disease, all these were seropositive. furthermore, of subjects infected with pathogenic zymodemes 94-100% were strongly seropositive, compared wi ...	1985	2857995
frequency distribution of entamoeba histolytica zymodemes in a rural south african population.	to determine the prevalence of entamoeba histolytica and its zymodeme distribution pattern, isoenzyme electrophoresis was performed on isolates of the parasite from 1381 individuals of the population of a semirural endemic area south of durban. about 10% of the population was found to harbour e histolytica. the zymodeme distribution was similar to that described in other surveys but two new zymodemes were observed, a non-pathogenic zymodeme xix and a pathogenic zymodeme xx. non-pathogenic zymode ...	1985	2857996
is that amoeba harmful or not?		1985	2858001
[tropical mimics; study of the feces is a must].		1985	2858059
isolation, purification, and partial characterization of an enterotoxin from extracts of entamoeba histolytica trophozoites.	soluble cell-free extracts of pathogenic entamoeba histolytica, as well as serum-free minimal media in which trophozoites are incubated, contain substances that cause the rapid rounding up and detachment of tissue-cultured monolayers of mammalian cells (cytopathic activity) and induce fluid secretion in ligated intestinal loops of indomethacin-pretreated rats (enterotoxic activity). a semiquantitative assay for the determination of the cytopathic activity based on the rate of detachment of tissu ...	1985	2858451
entamoeba histolytica: early progressive pathology in the cecum of the gerbil (meriones unguiculatus).	the early formation of amebic cecal lesions in the gerbil (meriones unguiculatus) was studied sequentially from 1 to 10 days post-inoculation. the earliest response of the cecal mucosa towards trophozoites of entamoeba histolytica was local release of interglandular and goblet cell mucin. trophozoites adhered to the surface epithelium or were trapped in the luminal mucus in the absence of epithelial cell damage. focal mucosal erosion occurred later, and was preceded by crypt hyperplasia, edema a ...	1985	2858986
n-acetyl-d-galactosamine-inhibitable adherence lectin of entamoeba histolytica. i. partial purification and relation to amoebic virulence in vitro.	adherence of axenic entamoeba histolytica, strain hm1-imss, to chinese hamster ovary (cho) cells is mediated by an amoebic adhesin that is inhibited by n-acetyl-d-galactosamine (galnac). the in vitro virulence for cho cells and human neutrophils (pmns) of four strains of axenic amoebae was strain hm1-imss greater than h303-nih = 200-nih greater than nonvirulent laredo (p less than .001). the hm1 strain had the greatest sensitivity to galnac-mediated inhibition of adherence (p less than .001). ga ...	1985	2859338
n-acetyl-d-galactosamine-inhibitable adherence lectin of entamoeba histolytica. ii. mitogenic activity for human lymphocytes.	a soluble protein preparation from virulent axenic amoebae (strain hm1-imss) has been demonstrated to be mitogenic for normal human lymphocytes; mitogenesis was optimal at 100 micrograms/ml when incubation was maintained for five to seven days with monocytes (p less than .001). after gel filtration chromatography mitogenic activity of fractions was associated with n-acetyl-d-galactosamine-inhibitable lectin activity (agglutination of chinese hamster ovary cells; p less than .001). lymphocyte pro ...	1985	2859339
entamoeba histolytica and e. invadens: sulfhydryl-dependent proteolytic activity.	sodium dodecyl sulfate (sds) and 2-mercaptoethanol (2-me) activated proteolytic enzymes present in extracts of entamoeba histolytica and e. invadens; sds (0.5%) and 2-me (1.4 and 715 mm) doubled the enzymatic activity when assayed on a stained insoluble substrate. urea (4 m) did not reduce this activity, suggesting that amebic proteases are stable in the above denaturant conditions. specific reagents for sulfhydryl (-sh) groups completely inhibited proteolytic activity regardless of ph. inhibiti ...	1985	2859367
structural bases of the cytolytic mechanisms of entamoeba histolytica.	the cellular bases of the powerful cytolytic activity of the human protozoan parasite entamoeba histolytica were explored by studying the effect of the virulent strain hm1:imss on epithelial monolayers of mdck cells using a combination of time-lapse microcinematography and transmission and scanning electron microscopy. early alterations of the epithelial cell membranes were detected by measuring changes in the transepithelial electrical resistance of mdck monolayers mounted in ussing chambers. t ...	1985	2859368
amoebic isoenzymes.		1985	2859504
urinary tract infection with entamoeba histolytica after ileocaecal bladder replacement.		1985	2859507
isolation and characterization of entamoeba histolytica mutants resistant to emetine.	isolation of emetine-resistant mutants of entamoeba histolytica is described. spontaneous rate of mutation, obtained from a luria-delbrück fluctuation test was 2.5 x 10(-7). the mutagenesis frequency obtained from the number of colonies grown in the presence of emetine, divided by the number of viable trophozoites inoculated in semisolid agar was less than or equal to 10(-7) and it was increased from 10 to 100 fold with ethyl methanesulfonate. two types of clones were isolated: one was cross-res ...	1985	2859522
tolerance of axenically cultured entamoeba histolytica and giardia lamblia to a variety of antimicrobial agents.	twelve antimicrobial agents were tested for use in axenic cultures of entamoeba histolytica and giardia lamblia. ten did not inhibit parasite growth at concentrations of at least two-fold above the effective antibacterial or antifungal levels. we have found the combination of piperacillin (500 micrograms/ml) plus amikacin (125 micrograms/ml) to be useful in axenization of a new strain of e. histolytica, eradication of multiply drug-resistant contaminants in cultures and prevention of bacterial g ...	1985	2859675
zymodemes expressing possible genetic exchange in entamoeba histolytica.	entamoeba histolytica was first seen by lösch (1875), and subsequently described by schaudinn (1903). since that time this organism has only been known to divide by binary fision. observations at molecular level now reveal possible genetic exchange in this species of amoeba.	1985	2859677
effects of cytochalasin b on na+ content and cell volume of entamoeba histolytica.	cells of entamoeba histolytica accumulated k+ and extruded na+ compared to the concentrations of those ions present in the growth medium. pinocytic activity, measured by the uptake of horseradish peroxidase of 125i-polyvinylpyrrolidone, was high (up to 0.3 ml/ml cells per h). upon addition of cytochalasin b, at a concentration (20 microm) that completely blocked pinocytosis, cells lost up to 40% of their na+ content within 90 min; k+ content was not affected or increased slightly compared to con ...	1985	2859891
entamoeba histolytica: purification of cathepsin b.	a cytotoxic cysteine proteinase with a molecular weight of 16,000 was isolated from axenically grown trophozoites of entamoeba histolytica. the enzyme was purified from frozen-thawed strain hm-1 by ion-exchange chromatography on deae-cellulose, organomercurial agarose affinity chromatography, and size-exclusion chromatography. the purified enzyme had proteinase activity that could be demonstrated on azocasein (ph 5), hemoglobin (ph 5), or carbobenzoxy-l-arginyl--l-arginyl-7-amino-4-trifluorometh ...	1985	2860002
vaccination of rabbits against entamoeba histolytica with aqueous suspensions of trehalose-dimycolate as the adjuvant.	rabbits were immunized with soluble entamoeba histolytica antigen with an aqueous suspension of trehalose-6,6'-dimycolate used as the adjuvant. induction of protective immunity in the immunized animals was demonstrated by enhanced humoral and cell-mediated immune responses and 100% survival after challenge. administration of soluble antigen only failed to induce a similar degree of protective immunity. trehalose-6,6'-dimycolate alone produced only a slight increase in nonspecific resistance.	1985	2860068
complement activation by antigenic fractions of entamoeba histolytica.	complement (c) activation induced by entamoeba histolytica in normal non-immune human serum was studied by testing in vitro the ability of different fractions of the trophozoites to cause c3 breakdown. whole trophozoites were found to activate both alternative and classical c pathways. the antibody-independent classical pathway (mgegta inhibitable) c activating capacity was found to greatly increase after disruption of the cell membrane by sonication. subsequent analysis after differential centr ...	1985	2860628
[invasive amebiasis (i): etiology, pathogenesis, epidemiology, and transmission. intestinal amebiasis and its complications].		1985	2860702
[invasive amebiasis (ii): extra-intestinal forms and complications. parasitological and serological diagnosis. treatment].		1985	2860704
[use of modified asami medium as a monoxenic culture medium for entamoeba histolytica].	this is a report about the monoxenic cultivation of entamoeba histolytica in asami culture medium. the culture amebae serve as material for antigen preparation, for intrahepatic infections in golden hamsters and also for microscopical observation, s. figs. 2 and 3.	1985	2860761
cytologic demonstration of entamoeba histolytica using immunoperoxidase techniques. report of two cases.	an indirect immunoperoxidase technique to detect entamoeba histolytica in cell samples from patients suspected to have amebiasis is described. using a rat antiserum specific for e. histolytica, the organism was clearly identified both in smears and in cell blocks. this immunoperoxidase technique seems to offer great possibilities for a specific, accurate and rapid identification of amebic infestation in diagnostic cytology.	1985	2860765
use of a monoclonal antibody in an enzyme immunoassay for the detection of entamoeba histolytica in fecal specimens.	an indirect enzyme-linked immunosorbent assay (elisa) was developed for the detection of entamoeba histolytica in human feces, using both a monoclonal antibody and rabbit antisera. it detected from less than 1 to 57 trophozoites of 6 e. histolytica strains. stool specimens were positive by elisa in 18 of 22 (82%) patients with e. histolytica and in 3 of 186 (2%) of patients without demonstrable e. histolytica in their stools. the latter included one from a child living near an asymptomatic cyst ...	1985	2860814
secretory hormones of entamoeba histolytica.	watery diarrhoea as distinct from dysentery is occasionally seen in intestinal amoebiasis, suggesting a component of intestinal secretion. to study the pathogenesis of this watery diarrhoea, we evaluated the effect of lysates of entamoeba histolytica on active intestinal electrolyte transport using rabbit ileum and rat colon studied by the ussing chamber-voltage clamp technique. amoebic lysates added to the ileal and colonic mucosal surfaces did not alter electrolyte transport; in contrast, addi ...	1985	2861068
human amoebiasis: the interaction of lymphocyte surface-bound immune complexes and pmns impairs t cell proliferative responses to e. histolytica mitogen.	in some of the sera from patients with amoebiasis circulating immune complexes are present which are thought to interact with lymphoid cells, enabling them to elicit a burst of oxygen consumption in pmns. the intensity of chemiluminescence is related to the presence of c3+ and fc igg+ cells in the lymphoid cell suspensions employed. the generation and release of highly reactive oxygen derivatives from pmns impair t lymphocyte proliferative responses to the e. histolytica mitogen. the authors sug ...	1985	2861159
enzyme linked immunosorbent assay for serodiagnosis of amoebiasis in children.		1985	2861160
chemotaxis by entamoeba histolytica.	a micropore membrane procedure to assay taxis by entamoeba histolytica is described and the results of studies of responses to a variety of soluble substances, bacteria, an rat colon washings using this procedure are reported. trophozoites migrated in blind well chambers through 8-micron pore size polycarbonate membranes but not nitrocellulose membranes up to 12 micron pore size. amoebae were attracted toward fresh axenic culture medium (tyi-s), an enzymatic hydrolysate of casein (trypticase), a ...	1985	2861283
incorporation and toxicity of 32p-orthophosphate and occurrence of polyphosphate in entamoeba trophozoites.	we explored the requirements of inorganic phosphate (pi), the incorporation of 32p-orthophosphate (32pi), and the occurrence of inorganic polyphosphate (polyp) in axenic entamoeba cultures. maximal population densities and growth rates of entamoeba histolytica trophozoites were attained in complete tp-s-1 medium. as 32pi concentration was increased in the medium, its own incorporation and the culture growth rate were progressively inhibited, especially in pi-deficient medium. polyp grains were f ...	1985	2861284
amebic colitis. preventing morbidity and mortality from fulminant disease.	uncomplicated amebic colitis is readily treated and has a mortality rate of less than 0.5%. complications necessitating surgical intervention develop in only 6% to 11% of patients with symptomatic disease. however, the mortality rate in these patients ranges from 40% to 100% and stems in part from delays in diagnosis and treatment. patients with known amebic colitis who show signs of systemic toxicity or of localized or generalized peritonitis are at high risk for complications; surgical consult ...	1985	2861601
infectious diarrhea. pathogenesis and risk factors.	our understanding of the pathogenesis of infectious, especially bacterial, diarrhea has increased dramatically. new etiologic agents, mechanisms, and diseases have become known. for example, escherichia coli serogroup o157 is now known to cause acute hemorrhagic colitis. also, e. coli serogroups that produce shiga toxin are recognized as etiologic agents in the hemolytic-uremic syndrome. the production of bacterial diarrhea has two major facets, bacterial-mucosal interaction and the induction of ...	1985	2861742
[anti-amebic effect of polyenic antibiotics].	all-union research technological institute of antibiotics and medical enzymes, leningrad. institute of epidemiology, virology and medical parasitology, ministry of health of the armenian ssr. the effect of polyenic antibiotics made in the ussr on development of e. histolytica and e. moshkovski was studied. the following antibiotics were used: levorin and its derivatives, mycoheptin, amphotericin b, amphoglucamine and nystatin. the antibiotics were compared with emetine and metronidazole. some dr ...	1985	2861787
interactions between trophozoites of entamoeba histolytica and cells of the immune system.	the interaction between entamoeba histolytica trophozoites and cells of the immune system was studied in vitro. both immune as well as non-immune mononuclear cells (mnc) to some extent were capable of exerting a cytotoxic effect on e. histolytica trophozoites. incorporation of immune serum directed against the trophozoites in the reaction mixture resulted in a marked increase in the killing capacity of mnc. highest killing was observed (96.7 +/- 1.04%) with mnc obtained from animals immunized wi ...	1985	2861805
improved method of producing amoebic liver abscesses in hamsters for screening of systemically active amoebicides.	an improved method of producing hepatic amoebiasis in hamsters has been developed. a serially liver-passaged strain of entamoeba histolytica from experimentally-infected hamsters was used for infection. small pieces from the infected liver were examined for the presence of amoebae and contained approximately 20,000 active trophozoites per piece. such liver pieces, when used as an inoculum, produced infection in all of the animals. the method has been successfully employed for screening of known ...	1985	2861806
[frequency of enteropathogenic micro-organisms isolated in children with and without acute diarrhea].		1985	2861829
entamoeba histolytica: impedance measurements and cytotoxicity in the presence of bepridil, verapamil, and cytochalasin d.	entamoeba histolytica, and invasive enteric protozoa, kills mammalian target cells by sequential adherence and cytolytic events. using platinum plate electrodes with an alternating current source placed in a wheatstone bridge circuit, the impedance (resistance to ion flow) of a cell suspension of axenic amebae (strain hm1-imss) was measured. the impedance of the amebic cell suspension, expressed as resistivity (in ohm-cm), was significantly greater than the test solution and increased with decre ...	1985	2862058
differential lectin agglutinability of virulent and avirulent entamoeba histolytica strains.		1985	2862104
electrophoretic isoenzymes of isolates of entamoeba histolytica before & after revival of their virulence.		1985	2862106
rapid polymerization of entamoeba histolytica actin induced by interaction with target cells.	within 5 s of challenge of entamoeba histolytica trophozoites with red blood cells (rbc), attachment and deformation of target cells occurred at multiple sites on the amoeba surface. many trophozoite-target interfaces were outlined with a ring of polymerized amoeba actin, revealed by rhodamine-phalloidin staining of glutaraldehyde-fixed and triton-x 100-extracted cells. the beginnings of phagocytic pseudopods rimmed many targets. the phagocytic membrane and underlying actin network grew uniforml ...	1985	2862217
attempts to infect germfree nu/nu mice with entamoeba histolytica.		1985	2862642
a seroepidemiological survey of amoebiasis in lucknow.		1985	2862724
intestinal invasive amebiasis: an experimental model in rodents using axenic or monoxenic strains of entamoeba histolytica.	a new experimental model for the production of early stages of invasive intestinal amebiasis in hamsters or guinea pigs with axenic or monoxenic cultures of entamoeba histolytica of the hm1:imss strain is reported. the model is called the washed-closed cecal loop, because it involves the washing out of the cecum contents of conventionally raised animals and the formation of a closed loop, into which amebas are inoculated. colonization occurred in all inoculated animals. after 48 hr, macroscopic ...	1985	2862801
[etiology of acute infantile gastroenteritis in gabon].	rotaviruses are the main etiology of acute diarrhoeas in gabonese children (11 to 30% according to age). salmonellae (11.4%), shigellae (7.1%) and e. histolytica (7.1%), isolated or associated with enterobacteria, e. coli (3%), giardia and strongyloides stercoralis (1.4%), yersinia enterocolitica (1%) and balantidium coli (0.5%) were also found, without cholera.	1985	2863005
[endocytosis of liposomes by entamoeba histolytica and transport of encapsulated molecules toward the cytoplasm].	we have examined the interaction of a highly phagocytosing cell: entamoeba histolytica with liposomes of different lipid compositions, and followed, by a semi-quantitative method, the intracellular fate of the entrapped molecules. liposomes containing a small molecule, 6-carboxyfluorescein, are first phagocytosed. then the encapsulated compound migrates from the vacuoles to the cytoplasm. liposomes containing macromolecular substances, such as fluorescent albumin or ferritin, are also phagocytos ...	1985	2863146
in vitro and in vivo interaction between trophozoites of entamoeba histolytica and gerbil lymphoid cells.	the in vitro and in vivo antiamoebic cytotoxic effects of peritoneal exudate cells and mesenteric lymph node lymphocytes of gerbils with cecal amoebiasis or those immunized with amoebic extract were investigated. a differential effect of the lymphoid cells against trophozoites of nonpathogenic and pathogenic strains of entamoeba histolytica was observed. nonpathogenic amoebae were more susceptible to killing by lymphoid cells than pathogenic amoebae in vitro and in vivo in infected or immunized ...	1985	2863217
interaction of human leukocytes and entamoeba histolytica. killing of virulent amebae by the activated macrophage.	capable effector mechanisms in the human immune response against the cytolytic, protozoan parasite entamoeba histolytica have not been described. to identify a competent human effector cell, we studied the in vitro interactions of normal human polymorphonuclear neutrophils, peripheral blood mononuclear cells (pbmc), monocytes (mc), and mc-derived macrophages with virulent axenic amebae (strain hmi-imss). amebae killed neutrophils, pbmc, mc, and mc-derived macrophages (p less than 0.001), without ...	1985	2863284
phospholipase a enzymes of entamoeba histolytica: description and subcellular localization.	cytolysis of target cells by entamoeba histolytica is a rapid, contact-dependent event that appears to involve calcium and amebic phospholipase a enzymes. there are two phospholipase a enzymes of e. histolytica: one calcium independent and optimally active at an acid ph, and a second calcium dependent and most active at an alkaline ph. the amebic calcium-dependent enzyme was inhibited by pharmacological antagonists that have been shown to reduce cytolysis of target cells by intact amebae: phosph ...	1985	2863316
effect of antagonists of calcium and phospholipase a on the cytopathogenicity of entamoeba histolytica.	the in vitro mechanisms by which entamoeba histolytica trophozoites lyse target chinese hamster ovary (cho) cells were examined. calcium chelators ethylenediaminetetraacetate and ethyleneglycol bis (beta-aminoethyl ether)-n,n'-tetraacetate (10 mm) inhibited amebic cytolysis of target cho cells (p less than .01). a putative antagonist of intracellular calcium flux, 8-(n,n-diethylamino)octyl-3,4,5-trimethoxybenzoate (tmb-8; greater than or equal to 250 microm), inhibited amebic adherence and cytol ...	1985	2863317
[native entamoeba histolytica infections].		1985	2863438
effect of lanosterol on the susceptibility of mastomys natalensis to entamoeba histolytica infection.		1985	2863883
entamoeba histolytica: electrophoretic isoenzyme patterns of strains and their virulence in the cecum of gerbils (meriones unguiculatus).	six strains of entamoeba histolytica isolated from humans were characterized by their electrophoretic isoenzyme patterns and by virulence in the cecum of mongolian gerbils (meriones unguiculatus). amebae from symptomatic cases of amebiasis were found to belong to zymodeme group ii, whereas strains isolated from asymptomatic infections belonged to zymodeme groups i or v. strains recently isolated from symptomatic cases of amebiasis were highly invasive in the cecum of gerbils. the most prominent ...	1985	2863988
[immunologic characterization of a monoclonal antibody specific for e. histolytica].		1985	2864744
detection of entamoeba histolytica in the pus of amoebic liver abscess by immunofluorescent technique.		1985	2865204
activation of the alternative and classical complement pathways by entamoeba histolytica.	entamoeba histolytica hm1 supported the activation of human alternative and classical complement pathways in the absence of ameba-reactive antibodies. nonimmune serum depleted of c1q and factor d (nhs s c1q + d) and reconstituted with c1q was able to specifically deposit c3b onto trophozoites and produce lysis. this activity was not modified by the absorption of serum on e. histolytica. serum depleted of factor b allowed c3b binding to amebae. serum devoid of c4 effected only small amounts of c3 ...	1985	2865212
a dot enzyme-linked immunosorbent assay for detection of antibodies against entamoeba histolytica.	a visual micro-dot enzyme-linked immunosorbent assay (elisa) based on detection of antibodies against soluble antigens of axenically grown cultures of entamoeba histolytica is described. the antigen was spotted on a nitrocellulose sheet, the unsaturated sites blocked with bovine serum albumin (bsa) and incubated with 3-fold dilutions of patient sera followed by incubation with protein a conjugated to peroxidase. enzymic activity was evidenced using the substrate 4-chloro-1-naphthol. a positive r ...	1985	2865313
entamoeba histolytica: lymphoreticular changes in gerbils (meriones unguiculatus) with experimentally induced cecal amebiasis.	antibody responses and changes in the lymphoreticular tissues of gerbils with experimental cecal amebiasis were studied from 5 to 60 days pi. changes in the cecum consisted of lymphoid follicle hyperplasia and depletion of lymphocytes, followed by follicle atrophy and histiocytosis. mesenteric lymphadenopathy, and histologic alterations in the lymph nodes paralleled the progressive development of amebic cecal lesions. early in the infection (5 to 10 days pi) mesenteric lymph nodes showed cortica ...	1985	2865345
purine salvage in entamoeba histolytica.	pulse-labeling of the nucleotide pool in entamoeba histolytica with radioactive precursors, and subsequent high performance liquid chromatographic (hplc) analysis of the radiolabeled nucleotides, indicate that e. histolytica is incapable of de novo synthesis of purine nucleotides. hypoxanthine, inosine and xanthine could not be converted to nucleotides in e. histolytica, which suggests the absence of interconversion between adenine nucleotides and guanine nucleotides through formation of imp. ad ...	1985	2865346
experimental infection of rhesus monkeys with entamoeba histolytica mimics human infection.	experimental infection of entamoeba histolytica was successfully established in rhesus monkeys (macaca mulatta). parasites of proven pathogenicity maintained through liver passage in hamsters were used in this study. laparotomized monkeys were inoculated intracecally/intrahepatically with trophozoites, or by oral administration of cysts (2000 cysts/ml). the cysts were isolated from an infected human stool sample. formation of typical amebic lesions in the inoculated tissues along with alteration ...	1985	2865401
[entamoeba histolytica and other intestinal parasites in an institution for the mentally retarded].		1985	2865836
entamoeba histolytica, antigens and amoebiasis.		1985	2866068
inhibition of human monocyte locomotion by products of axenically grown e. histolytica.	the supernatant fluid of axenically grown e. histolytica inhibits chemotaxis, chemokinesis and random mobility of human mononuclear phagocytes (mp) as measured in boyden chambers. human polymorphonuclear phagocytes (pmn) locomotion is apparently unaffected. the factor was found in comparable amounts in the supernatant fluid of axenic cultures of four e. histolytica strains that differed in their human pathogenicity and virulence, as well as in two entamoebas non-pathogenic for man. this dialysab ...	1985	2866481
[culture methods in the diagnosis of intestinal parasitic protozoans in man, particularly entamoeba histolytica].		1985	2866849
multilayer-enzyme linked immunosorbent assay (ml-elisa) for detection of entamoeba histolytica trophozoite coproantigen.	multilayer elisa (ml-elisa) method for entamoeba histolytica trophozoite coproantigen was developed for diagnosis of amoebiasis and its usefulness vis-a-vis microscopic examination analysed for paired specimens from 87 patients from lucknow and hyderabad, india. all the stool specimens positive for trophozoites were also found to be positive in ml-elisa. out of a total 32 samples positive for cysts in microscopy 21 (65.62%) gave positive reactions for coproantigen. results on 26 samples drawn fr ...	1985	2867033
which amoebae are harmful?		1985	2867035
acanthamoeba, an opportunistic microorganism: a review.	granulomatous amebic encephalitis due to acanthamoeba spp. usually occurs in chronically ill and debilitated individuals. some of these patients may have received immunosuppressive therapy. another infection due to acanthamoeba spp. has been corneal ulcerations which usually occur after minimal trauma to the corneal epithelium (1). in contrast, primary amebic meningoencephalitis due to naegleria fowleri usually occurs in healthy, young individuals with a history of swimming in heated swimming po ...	1985	2867047
[short-term treatment of protozoonoses with tiberal].		1985	2867648
the biochemistry and functional morphology of the entamoeba.		1985	2409275
routine procedures for examination of stool and blood for parasites.	the diagnosis of a parasitic infection depends upon demonstration of the organism in stool, blood, or tissue specimens, and a variety of relatively simple procedures is available for this purpose. in this article, the author describes those procedures that are most frequently used on a routine basis.	1985	2410850
[translation of rna obtained from entamoeba histolytica and its characterization of a specific monoclonal antibody].		1985	2413554
histopathological diagnostics of liver amoebiasis in cambodia.	results of histopathological examination of a solitary chronical amoebic abscess in the liver of a patient from cambodia are described. the abscess was at a proliferously reparative phase and entamoeba histolytica was detected at the border between the necrosis and inner wall of abscess, as well as in the proper non-specific granulation tissue of the inner layers of abscess capsule. a differential diagnosis of the amoebic abscess from similar parasitary or pseudoparasitary liver lesions and diff ...	1985	2417921
antibody response of patients harbouring different zymodemes of entamoeba histolytica to homologous and heterologous antigens.	entamoeba histolytica isolates characterized into four zymodemes were used as antigens in the ifat; with 17 sera obtained from patients harbouring different amoebic isoenzyme groups. the method of cultivation used was found to influence the ease of reading the ifat. reactivity of serum samples, obtained from the selected patients (i.e. with varied forms of amoebiasis), against homologous and heterologous antigens did not reveal any antigenic differences among amoebic zymodemes. the present resul ...	1985	2418210
adherence of entamoeba histolytica trophozoites to rat and human colonic mucosa.	we studied the adherence of [3h]thymidine-labeled axenic entamoeba histolytica (strain hm1-imss) to in vitro preparations of rat and human colonic mucosa. studies were performed with fixed or unfixed rat colonic mucosa, unfixed rat mucosa exposed to trypsin, unfixed rat submucosa, and fixed human colonic mucosa. twenty percent of the amebae adhered to fixed rat colonic mucosa; adherence was specifically inhibited by n-acetyl-d-galactosamine (galnac), galactose, and asialofetuin. the adherence of ...	1985	2580787
subcellular fractionation of amoebapore and plasma membrane components of entamoeba histolytica using self-generating percoll gradients.	separation and initial characterization of subcellular organelles from entamoeba histolytica have been achieved by the use of self-generating percoll gradients. adequate resolution was obtained within one hour of amoeba homogenization. the ion-channel forming activity, amoebapore, was found associated with highly dense, small diameter particles, that were resolved from the numerous digestive vacuoles, and the plasma membrane. following iodination of intact trophozoites with lactoperoxidase, the ...	1985	2581133
fluorometric analysis of transferable membrane pores.	when pore-forming factors insert into the hyperpolarized membranes of lipid vesicles, ion gradients are rapidly equilibrated, effecting complete depolarization. this process can be conveniently followed with a potentiometric cyanine dye. the generality of the method is demonstrated by applications to three diverse materials. the well-studied gramicidin channel is used to demonstrate that the method is sensitive down to concentrations of 10(-12)m. an extract from the shark repellent skin secretio ...	1985	2581609
molecular mechanisms of cytotoxicity mediated by entamoeba histolytica: characterization of a pore-forming protein (pfp).		1985	2868017
detection of amoebic antigen by enzyme linked immuno-sorbent assay (elisa).		1985	2868123
growth studies on axenic entamoeba histolytica and naegleria fowleri.		1985	2868550