Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [preliminary observation on the effect of thymic peptide in the experimental infection of mice with mycobacterium leprae]. | 1985 | 2939979 | |
| [detection of antibodies in blood from the ear lobes of leprosy patients by enzyme-linked immunosorbent assay--a preliminary report]. | 1985 | 2939997 | |
| cellular immunity in lepromatous and tuberculoid leprosy. | the depression of cellular immunity in lepromatous patients is not understood. while the blood monocytes of leprosy patients appear to be activated normally by lymphokines, t cell proliferation and production of lymphokines in response to mycobacterium leprae are impaired in lepromatous patients. attempts to restore responsiveness in cells from these patients have been unsuccessful in our hands. the addition of exogenous il-2 to leukocyte cultures does not appear to restore responsiveness to m. ... | 1985 | 2935494 |
| interleukin-2-induced t-cell response to m. leprae in lepromatous leprosy: reversion of a suppressor mechanism or expansion of a small m. leprae-reactive t-cell pool? | 1985 | 2935587 | |
| the influence of immunosuppression and immunodeficiency on infections with leprosy and tuberculosis. | 1985 | 2987376 | |
| hydrogen peroxide and superoxide production by peripheral blood monocytes in leprosy. | susceptibility to infection with mycobacterium leprae, the causative organism of leprosy, is the result of a defect in cell-mediated immunity (cmi). the co-operation of macrophages and t lymphocytes is known to be essential for competent cmi response. in this study we have examined peripheral blood monocytes from a range of leprosy patients in an attempt to identify a possible defect in macrophage function. the ability of these cells to produce hydrogen peroxide and superoxide, two bactericidal ... | 1985 | 2988833 |
| human monoclonal antibodies against mycobacterium leprae. | human hybridomas were constructed which produce antibodies against three different extracts of mycobacterium leprae. a thioguanine-resistant (thgr), ouabain-resistant (ouar), human lymphoblastoid cell line, kr-4, was hybridized with epstein-barr virus-transformed cell lines from lepromatous leprosy patients with fusion frequencies of greater than 10(-5). non-epstein-barr virus-transformed donor cells fused at much lower rates (less than 2 x 10(-7]. hybrids were selected in medium containing hypo ... | 1985 | 2989177 |
| defective cell-mediated immunity in leprosy: failure of t cells from lepromatous leprosy patients to respond to mycobacterium leprae is associated with defective expression of interleukin 2 receptors and is not reconstituted by interleukin 2. | patients with lepromatous leprosy (ll) but not borderline tuberculoid leprosy (bt) have defective cell-mediated immune responses to mycobacterium leprae, despite normal responses to other stimuli, as judged by in vivo skin testing and in vitro lymphocyte transformation. to investigate the basis of the immune defect in ll patients, we studied the ability of patient mononuclear leukocytes to produce interleukin 1 (il 1) and interleukin 2 (il 2) upon stimulation with m. leprae, and determined the a ... | 1985 | 2989366 |
| activation of human monocytes in leprosy. | in leprosy, the common etiologic agent is the same mycobacterium leprae, but the clinical manifestations are various, including the tuberculoid and lepromatous types. in tuberculoid type leprosy, macrophages in the granuloma differentiate into epithelioid cells; in the lepromatous type, in contrast, they differentiate into lepra cells containing multiple m. leprae. thus host factors, which regulate macrophage activities, determine the type of leprosy. to understand such regulation of macrophage ... | 1985 | 2989662 |
| evidence for the activity of rifampin on the neuropathy of foot pad-inoculated mice with mycobacterium leprae. | 1985 | 2995517 | |
| epstein-barr virus-transformed b cell lines present m. leprae antigens to t cells. | we have investigated whether or not epstein-barr virus-transformed lymphoblastoid b cell lines (ebv-blcl) are able to present mycobacterium leprae (m. leprae) to antigen-reactive t cell lines and clones. such ebv-blcl would provide us with a homogeneous and unlimited source of antigen-presenting cells. antigen-triggered proliferation of t cells has been studied with co-cultures either with autologous or allogeneic irradiated ebv-blcl. our results show that ebv-blcl are able to present m. leprae ... | 1985 | 3001928 |
| genes for the protein antigens of the tuberculosis and leprosy bacilli. | the lambda gt 11 expression vector permitted us to survey protein antigens of mycobacterium leprae and mycobacterium tuberculosis expressed in escherichia coli. using monoclonal antibodies, recombinant clones were detected producing three major antigens of m. tuberculosis and five major protein antigens of m. leprae. these recombinant antigens produced in e. coli should prove useful for diagnosis, epidemiology and possibly the development of recombinant mycobacterial vaccines. | 1985 | 3006821 |
| monoclonal antibodies against mycobacterial antigens. | the world health organization (who) scientific working groups on the immunology of leprosy (immlep) and tuberculosis (immtub) recently organized two international workshops designed to characterize the specificity and reaction patterns of approximately 55 murine monoclonal antibodies (mabs) which had been raised against mycobacterium leprae or mycobacterium tuberculosis and submitted to the immlep and immtub monoclonal antibody banks. | 1985 | 25291216 |
| adenylate kinase activity in mycobacterium leprae. | adenylate kinase (atp:amp phosphotransferase, ec 2.7.4.3) was detected in partially purified preparations of cell-free extracts of mycobacterium leprae. the apparent km values of m. leprae adenylate kinase for adp and mg2+ were 1 x 10(-4) m, respectively. the enzyme was heat-labile: loss of activity by 80% at 45 degrees c and over 90% at 60 degrees c occurred within 5 min. m. leprae adenylate kinase was distinct from armadillo adenylate kinase in respect of affinity for substrate and heat-sensit ... | 1986 | 3011966 |
| construction of genomic libraries of mycobacterial origin: identification of recombinants encoding mycobacterial-specific proteins. | a complete genomic library from mycobacterium vaccae (2785 recombinants) and a partial genomic library of m. leprae and bcg (300 and 1750 clones, respectively) were constructed in the plasmid pbr322. bam hi was selected as the restriction endonuclease for obtaining dna cleavage products. evidence was obtained for limited expression of the cloned mycobacterial dna inserts in escherichia coli. a recombinant has been identified which codes for antigen immunoreactive with rabbit anti-m. leprae antib ... | 1986 | 3018107 |
| cloning and expression of mycobacterium bovis bcg dna in "streptomyces lividans". | the ability of "streptomyces lividans" to use the expression signals of genes from mycobacterium bovis bcg was tested in vivo by using gene fusions. random dna fragments from m. bovis bcg were inserted into promoter-probe plasmids in escherichia coli and in "s. lividans." comparison with promoter activity detected with random dna fragments from the respective hosts suggested that "s. lividans" efficiently utilizes a high proportion of mycobacterial promoters, whereas a smaller fraction are expre ... | 1986 | 3020007 |
| t90/44 (9.3 antigen). a cell surface molecule with a function in human t cell activation. | t90/44 is a cell surface antigen which is present on human t cells of the helper and cytotoxic subsets and which binds the 9.3 monoclonal antibody (9.3 mab). it is expressed in the form of 90-kda disulfide-bonded dimers of a 44-kda polypeptide and of free 44-kda subunits. the function of t90/44 was investigated in a series of t cell function assays. 9.3 mab was found to inhibit the activation of class ii-restricted cloned t helper cells derived from leprosy patients and reactive with m. leprae a ... | 1986 | 3021470 |
| separation of mycobacterium leprae from contamination with armadillo-liver-derived "pigment" particles. | mycobacterium leprae isolated from armadillo liver by the widely used immlep protocol is sometimes contaminated with a particulate "pigment." this paper describes a simple, efficient, and rapid method for purifying large quantities of contaminated bacteria, which may readily be used as an additional step added at the end of the protocol when necessary. the process involves a discontinuous percoll gradient and generates an essentially pure fraction containing greater than 90% of the original bact ... | 1986 | 3029249 |
| mycobacterium leprae fails to stimulate phagocytic cell superoxide anion generation. | mycobacterium leprae is an intracellular pathogen that is ingested by and proliferates within cells of the monocyte/macrophage series. mechanisms by which intracellular pathogens resist destruction may involve failure to elicit a phagocyte "respiratory burst" or resistance to toxic oxygen derivatives and lysosomal enzymes. we have studied the ability of m. leprae and mycobacterium bovis bcg to stimulate the generation of superoxide anion (o2-) in vitro by human blood neutrophils and monocytes an ... | 1986 | 3002986 |
| in vivo repackaging of recombinant cosmid molecules for analyses of salmonella typhimurium, streptococcus mutans, and mycobacterial genomic libraries. | strains of escherichia coli k-12 were constructed that permitted the amplification of in vitro-packaged recombinant cosmid-transducing particles by in vivo repackaging of recombinant cosmid molecules. thermal induction of these thermoinducible, excision-defective lysogens containing recombinant cosmid molecules yielded high titers of packaged recombinant cosmids and low levels of pfu. these strains were used to amplify packaged recombinant cosmid libraries of mycobacterium leprae, mycobacterium ... | 1986 | 2937735 |
| suppressor t lymphocytes from lepromatous leprosy skin lesions. | the immune response in leprosy forms a spectrum with lepromatous leprosy patients exhibiting specific unresponsiveness to antigens of mycobacterium leprae. this unresponsiveness is thought to be related to the prevalence of t8-positive lymphocyte in these lepromatous lesions. to analyze the immunoregulatory function of these t8 cells, we developed simple procedures to extract lymphocytes from skin biopsy specimens of patients with leprosy. these lymphocytes were sorted for t8 and t4 positive cel ... | 1986 | 2944966 |
| [intercellular relations in the immunology of leprosy]. | 1986 | 2951961 | |
| a follow up of t-cell subsets and of anti-m. leprae antibody titer as measured by the fla-abs test in melanesian leprosy patients under polychemotherapy. | melanesian leprosy patients from new caledonia were studied for the following parameters during the course of polychemotherapy: peripheral blood t-cell subsets, as identified in an immunofluorescence assay with monoclonal antibodies okt3 ("pan-t"), okt4 ("helper/inducer"), and okt8 ("cytotoxic-suppressor"), and anti-mycobacterium leprae antibodies in the serum, as measured by the fluorescent leprosy antibody absorption test. a group of melanesian healthy subjects with no known exposure to m. lep ... | 1986 | 2940306 |
| genetically restricted suppressor t-cell clones derived from lepromatous leprosy lesions. | leprosy is a spectral disease in which immune responses to mycobacterium leprae correlate with the clinical, bacteriological and histopathological manifestations of disease, so study of its pathology provides insights into immunoregulatory mechanisms in man. at the tuberculoid pole, patients have few lesions in the skin which contain rare organisms and are able to mount strong cell-mediated immune responses to m. leprae antigens. in contrast, at the lepromatous pole, patients have disseminated s ... | 1986 | 2942780 |
| [preliminary study on the serological activity of phenolic glycolipid and its application to leprosy]. | 1986 | 2943444 | |
| use of synthetic glycoconjugates containing the mycobacterium leprae specific and immunodominant epitope of phenolic glycolipid i in the serology of leprosy. | the high specificity of phenolic glycolipid i (pg-i) in the identifying individuals with leprosy appears to be attributable to the species-specific trisaccharide region of the molecule. synthetic glycoconjugates were produced by coupling the corresponding terminal mono- or disaccharide to bovine serum albumin by reductive amination. conjugates which contained only the terminal sugar maintained in its pyranose form, the terminal disaccharide with only the terminal sugar in its pyranose form and t ... | 1986 | 2431812 |
| [morphologic structure of mycobacterium leprae]. | 1986 | 2445724 | |
| serological tests for leprosy. | 1986 | 2869264 | |
| expression of mycobacterium leprae genes from a streptococcus mutans promoter in escherichia coli k-12. | genomic libraries of mycobacterium leprae dna partially digested with pst i were constructed in the expression vector pya626, which contains the promoter region from the streptococcus mutans gene encoding aspartate beta-semialdehyde dehydrogenase, which is very efficiently expressed in escherichia coli. we have detected several clones that complement a mutation in the citrate synthase gene of e. coli. southern blot analysis demonstrated that the complementing dna was m. leprae dna. sodium dodecy ... | 1986 | 2869492 |
| biochemical studies on mycobacterium leprae. | very little information is available on the basic biology of mycobacterium leprae. it is not known why the organism fails to grow in bacteriological media or in cell cultures and why it has an unusual predilection for certain tissues in the human host where cells derived from the neural crest occur (e.g. skin, peripheral nerves, adrenal medulla). biochemical studies have revealed that m. leprae contains an unusual form of the enzyme diphenoloxidase which has not been detected in other mycobacter ... | 1986 | 2874208 |
| hansen's disease (leprosy). diagnosis by aspiration biopsy of lymph nodes. | a 61-year-old male native of mexico presented with generalized enlargement of lymph nodes. fine needle aspiration (fna) biopsy established lepromatous leprosy as the cause of the lymphadenopathy. the cytologic findings included abundant, frequently multinucleated histiocytes (globus cells), the cytoplasm of which showed multiple vacuoles; cytoplasmic membrane-bound vacuoles were seen free in the background. the vacuoles contained large numbers of acid-fast bacilli. globus cells, while characteri ... | 1986 | 2421510 |
| m. leprae and ppd-triggered t cell lines in tuberculoid and lepromatous leprosy. | proliferative responses of peripheral blood mononuclear cells (pbmc) to mycobacterium leprae and bacillus calmette guerin-derived purified protein derivative (ppd) were studied in the presence or absence of interleukin 2 (il 2) in high m. leprae responders (tuberculoid leprosy patients and healthy subjects) and low m. leprae responders (lepromatous leprosy patients). high responders in most cases developed a strong proliferative response to both antigens in the absence of il 2. additional il 2 a ... | 1986 | 2422278 |
| investigation of antigen cross-reactivity of mycobacterium leprae-reactive murine t-cell lines and clones. | inguinal lymph node lymphocytes from balb/c mice immunized intradermally with 10(8) 60co-irradiated mycobacterium leprae were cloned by limiting dilution culture. in general, cloned t-cell lines exhibited helper type activity producing interleukin-2, macrophage activation factor and gamma-interferon and lines were further characterized in terms of their cross-reactivities with other species of mycobacteria. m. leprae clones derived after a period of in vitro restimulation were found to cross-rea ... | 1986 | 2423443 |
| [staining of mycobacterium leprae: comparison between the lapeyssonnie and causse technic on the one hand and thelep's technic on the other in the evaluation of the bacteriological index]. | in the present review, two staining techniques have been compared to evaluate bacteriological index: the lapeyssonnie and causse's technique, used in french speaking africa, and the one recommended by the o.m.s. scientific panel on chemotherapy of leprosy, thelep. it appears that the former facilitates the finding of a greater number of bacilli. more efficient, it is also easier and faster than the latter one and, consequently, more adapted to logistic constraints attached to the fight against e ... | 1986 | 2425213 |
| cloned suppressor t cells from a lepromatous leprosy patient suppress mycobacterium leprae reactive helper t cells. | leprosy is a chronic infectious disease caused by mycobacterium leprae. a characteristic feature of the disease is its remarkable spectrum of clinical symptoms correlating with the cellular immune responsiveness of the patient. at one pole of this spectrum are tuberculoid patients displaying both acquired cell-mediated immunity and delayed type hypersensitivity against the bacillus. at the other pole are lepromatous patients which show a specific t-cell unresponsiveness against m. leprae. in bet ... | 1986 | 2426597 |
| specific antigen and antibody to mycobacterium leprae in the cryoprecipitate of a patient with lucio phenomenon. | using a sensitive and specific enzyme-linked immunosorbent assay (elisa) assay we showed that the cryoglobulins of a patient with lucio phenomenon contain phenolic glycolipid i antigen and a specific antibody. | 1986 | 2429360 |
| in situ and in vitro characterization of the cellular immune response in erythema nodosum leprosum. | we sought to evaluate cell-mediated immune responses in erythema nodosum leprosum (enl), a reactional state occurring in lepromatous leprosy. skin biopsies from patients with leprosy were studied with monoclonal antibodies against t lymphocyte antigenic determinants, interleukin 2 (il 2), and il 2 receptors (tac) by using immunoperoxidase staining of frozen sections. peripheral blood lymphocytes from 18 enl patients were tested in vitro for lepromin-induced suppression of con a stimulation. seri ... | 1986 | 2416837 |
| a rapid silver staining method for identification of mycobacterium leprae in histologic sections. | a few methods have been reported for the purpose of staining mycobacterium leprae in paraffin sections, including fite oil fuchsin method, auramine-rhodamine method, and blanco-fite silver method. among these staining techniques, fite oil fuchsin method and auramine-rhodamine method are popular. however, the blanco-fite silver method takes approximately 20 days. therefore, we developed a new procedure for rapid identification of m. leprae in paraffin sections using another silver solution and fo ... | 1986 | 2435021 |
| efficient mapping of protein antigenic determinants. | a recombinant dna expression strategy has been used to deduce the amino acid sequences of six different antigenic determinants in a single protein of mycobacterium leprae, the etiologic agent of leprosy. the gene encoding the m. leprae 65-kda antigen was sequenced and a lambda gt11 gene sublibrary was constructed with fragments of the gene. recombinant dna clones producing specific antigenic determinants were isolated by screening with monoclonal antibodies, and the sequences of their insert dna ... | 1986 | 2428046 |
| [anti-m. leprae antibodies and the serology of leprosy]. | 1986 | 2436432 | |
| solid phase peptide synthesis of epitopes that react with monoclonal antibodies to the 65,000 dalton protein of mycobacterium leprae. | 1986 | 2437421 | |
| characterization of mycobacterial species specificity of 14 separate epitopes which reacted with monoclonal antibodies to the 65,000 molecular weight protein molecule of mycobacterium leprae. | 1986 | 2437422 | |
| separate antigenic determinants on cell wall associated carbohydrate antigens of mycobacterium leprae defined with monoclonal antibodies. | monoclonal antibodies (mabs) raised against mycobacterium leprae sonicate defined two different determinants on related, cell-wall-associated, carbohydrate antigens common to m. leprae, m. bovis (bcg), and m. tuberculosis. antigen inhibition elisa and antigen capture assays demonstrated that the two antigens were present in a cell-wall fraction, m. leprae resonicate. there was species variation in the distribution of the antigens; the 4.5-6 kd antigen was more abundant in m. tuberculosis and m. ... | 1986 | 2434585 |
| staining of mycobacterium leprae in epoxy resin sections. | 1986 | 2419364 | |
| molecular localization and polymorphism of hla class ii restriction determinants defined by mycobacterium leprae-reactive helper t cell clones from leprosy patients. | mhc class ii molecules carry the restriction determinants (rds) for antigen presentation to antigen-specific th lymphocytes. this restriction of t cell activation endows those molecules with a key role in the induction and regulation of antigen-specific immune responses. moreover, class ii molecules are the products of class ii immune response (ir) genes. the polymorphism of these ir genes leads to genetically controlled differences in immuneresponsiveness between different individuals. an impor ... | 1986 | 2431092 |
| prevalence and specificity of the enhancing effect of three types of interleukin 2 on t cell responsiveness in 97 lepromatous leprosy patients of mixed ethnic origin. | peripheral blood mononuclear cells from 97 predominantly lepromatous leprosy patients and 11 control subjects were tested in a lymphoproliferative assay for response to mycobacterium leprae (whole and sonicated), and sonicated m. vaccae, m. tuberculosis, and m. scrofulaceum, in the presence and absence of three types of interleukin 2 (il-2) (crude, purified, and recombinant). il-2 enhanced the response to sonicated m. tuberculosis and m. leprae organisms more often in patients than in control su ... | 1986 | 2426020 |
| a modification of fite's stain for demonstration of m. leprae in tissue sections. | 1986 | 2427624 | |
| viability of mycobacterium leprae: a comparison of morphological index and fluorescent staining techniques in slit-skin smears and m. leprae suspensions. | in a comparison of the estimation of mycobacterium leprae viability by morphology and the fluorescent vital dyes fda/eb and r123/eb, the latter techniques were more satisfactory using suspensions and slit-skin smears of m. leprae bacilli. both fda/eb and r123/eb seem to more accurately reflect viability after freeze/thaw cycles and heating, and are able to detect lower percentages of viable bacilli. in addition, the fluorescent vital dye techniques are both simple and less open to subjective int ... | 1986 | 2427626 |
| a preliminary study on serological activity of a phenolic glycolipid from mycobacterium leprae in sera from patients with leprosy, tuberculosis and normal controls. | 1986 | 2427906 | |
| hla class-ii-restricted mycobacterium leprae-reactive t-cell clones from leprosy patients established with a minimal requirement for autologous mononuclear cells. | this report describes an effective method for the cloning of mycobacterium leprae-reactive t lymphocytes with epstein-barr-virus transformed autologous b cells as antigen-presenting cells. the two advantages of this method are that it drastically reduces the number of autologous peripheral blood mononuclear cells (less than 10(7) cells) needed to obtain and propagate these t-cell clones (tlc), and that it enables us to expand individual tlc to large numbers of cells (greater than 10(8)). thus th ... | 1986 | 2422737 |
| learning from leprosy: a perspective on immunology and the third world. | 1986 | 2423599 | |
| periodic acid-ethanol gelatin methenamine silver stain for demonstrating m. leprae and myelin in peripheral nerve fibers of leprosy patients. | we have introduced the periodic acid-ethanol gelatin methenamine silver stain for the simultaneous demonstration of mycobacterium leprae and myelin in the peripheral nerves of leprosy patients. | 1986 | 2423620 |
| mycobacterium leprae-specific protein antigens defined by cloned human helper t cells. | leprosy displays a remarkable spectrum of symptoms correlating with the t-cell-mediated immune reactivity of the host against the causative organism, mycobacterium leprae. at one pole of this spectrum are lepromatous leprosy patients showing a m. leprae-specific t-cell unresponsiveness; at the other are tuberculoid leprosy patients displaying both acquired immunity and delayed-type hypersensitivity against m. leprae which are thought to be conferred by helper t (th) cells. because well-defined m ... | 1986 | 2417123 |
| human t-cell clones with reactivity to mycobacterium leprae as tools for the characterization of potential vaccines against leprosy. | t-cell clones with the t4 phenotype were established from patients with tuberculoid leprosy. the antigen reactivity of these clones ranged from stringent specificity for mycobacterium leprae to broad cross-reactivity with other mycobacteria. killed m. leprae had a weak stimulatory capacity which could be enhanced by ultrasonication. among the three candidate antileprosy vaccines, m. leprae, m. bovis bcg, and the icrc (indian cancer research center) strain, the last was superior in stimulating cr ... | 1986 | 3081446 |
| characteristics of human t-cell clones from bcg and killed m. leprae vaccinated subjects and tuberculosis patients. recognition of recombinant mycobacterial antigens. | 1986 | 3106732 | |
| peptides as potential immunodiagnostic reagents to detect mycobacterial infections. | 1986 | 3106733 | |
| analysis of a leprosy-specific antibody epitope. | 1986 | 3106734 | |
| lepromin stimulates interleukin-2 production and interleukin-2 receptor expression in situ in lepromatous leprosy patients. | 1986 | 3106736 | |
| structure and antigenicity of the phosphorylated lipopolysaccharide antigens from the leprosy and tubercle bacilli. | a family of major arabinose- and mannose-containing phosphorylated lipopolysaccharides was isolated from mycobacterium leprae and mycobacterium tuberculosis. the only antigenic member of the family, lipoarabinomannan (lam)-b, was purified by anion exchange and gel filtration chromatography in detergent and recovered in large quantities (15 mg/g of bacteria). it yielded a broad diffuse band on polyacrylamide gel electrophoresis but appeared homogeneous by this criterion and gel filtration. beside ... | 1986 | 3091602 |
| immunity to leprosy. iii. the in vitro induction of b lymphocyte proliferation by mycobacteria. | the development of murine proliferative response assays has been initiated to begin to evaluate t-lymphocyte responses to the antigens of mycobacterium leprae. in this study, m. leprae and 13 related strains of mycobacteria have been tested for stimulatory effects in proliferation assays using murine spleen, thymus or lymph node cultures. a number of mycobacteria were found to directly stimulate the proliferation of spleen and lymph node cells of all mouse strains tested including c3h/hej mice. ... | 1986 | 3086467 |
| lack of mycobacterium leprae-specific uptake in schwann cells. | among mycobacteria, mycobacterium leprae have a unique property to infect peripheral nerves, which is the cause of a variety of debilities seen in leprosy. the possibility of selective uptake of m. leprae by schwann cells was studied using a rat schwannoma cell line 33b and rat sciatic nerve-derived schwann cells. m. leprae were phagocytosed by 33b cells but so also were seven other mycobacteria ("mycobacterium w," bcg, m. tuberculosis h37rv, m. nonchromogenicum, m. vaccae, icrc bacillus, and m. ... | 1986 | 3086468 |
| concepts behind the development of multiple drug therapy regimens in leprosy. | 1986 | 3295437 | |
| ciprofloxacin (4-quinolone) and mycobacterium leprae. | 1986 | 2943954 | |
| biochemical studies on mycobacterium leprae. | very little information is available on the basic biology of mycobacterium leprae. it is not known why the organism fails to grow in bacteriological media or in cell cultures and why it has an unusual predilection for certain tissues in the human host where cells derived from the neural crest occur (e.g. skin, peripheral nerves adrenal medulla). biochemical studies have revealed that m. leprae contains an unusual form of the enzyme diphenoloxidase which has not been detected in other mycobacteri ... | 1986 | 3098865 |
| a simplified serological test for leprosy based on a 3,6-di-o-methylglucose-containing synthetic antigen. | the recent advent of synthetic antigens containing the mycobacterium leprae-specific epitope, 3,6-di-o-methyl-beta-d-glucopyranoside, has allowed the development of simple specific serological tests for leprosy. the incorporation of one such product, 8-carbonyloctyl o-[4-o-(3,6-di-o-methyl-beta-d-glucopyranosyl)-alpha-l- rhamnopyranoside]-bsa, into a simple "spot" test, diffusion-in-gel enzyme-linked immunosorbent assay (elisa), allowed an over 90% detection rate of untreated lepromatous leprosy ... | 1986 | 3080916 |
| local and systemic effects of intradermal recombinant interferon-gamma in patients with lepromatous leprosy. | evidence that interferon-gamma may be a physiologic macrophage-activating factor, and that macrophage activation may be defective in lepromatous leprosy, led us to test the effects of intradermal injection of low doses of recombinant interferon-gamma in six patients with this disease. interferon-gamma, 1 or 10 micrograms, was administered daily by jet gun for three days into a single cutaneous lesion. a biopsy specimen was taken from the injection site on the sixth study day and compared with sp ... | 1986 | 3086725 |
| detection of mycobacterial antigens in leprosy serum immune complex. | the antigens from immune complexes of sera from patients with mycobacterial diseases were released by sodium dodecyl sulfate. the antigenic activity of the released proteins was tested by agar gel diffusion and immunoelectrophoresis. this simple method provided direct evidence for the presence of mycobacterial antigens in the immune complexes of sera from patients with leprosy and tuberculosis. | 1986 | 3088033 |
| strategies in the development of new drugs and drug combinations against leprosy, demonstrated on the example of folate and gyrase inhibitors. | 1986 | 3033412 | |
| possible role of helper and cytolytic t cells in mycobacterial infections. | 1986 | 2952852 | |
| hla class ii restricted helper and suppressor clones reactive with mycobacterium leprae. | 1986 | 2952853 | |
| effect of recombinant interferon-gamma on hydrogen peroxide-releasing capacity of monocyte-derived macrophages from patients with lepromatous leprosy. | monocyte-derived macrophages from 14 patients with lepromatous leprosy respond to rifn-gamma with an enhanced secretion of h2o2 in a fashion similar to that of cells obtained from normal donors. the activation is not dependent on the cutaneous bacterial index, the length of treatment, or the stage and activity of the disease. h2o2 release can be triggered in these cells both by phorbol myristate acetate and by intact irradiated mycobacterium leprae. uptake of m. leprae by both normal donors' and ... | 1986 | 3088117 |
| mycobacterium leprae-specific lyt-2+ t lymphocytes with cytolytic activity. | mice were immunized intradermally with 10(7) irradiated mycobacterium leprae organisms, and draining lymph nodes were collected after 4 weeks. lymph node cells were restimulated in vitro with soluble m. leprae antigen and accessory cells. the resulting t-cell line was propagated in vitro in the presence of m. leprae antigen, accessory cells, and interleukin-2-containing supernatants from concanavalin a-stimulated rat spleen cells. long-term cultured t cells were thy-1+ l3t4- lyt-2+ as revealed b ... | 1986 | 3096892 |
| concepts behind the development of multiple drug therapy regimens in leprosy. | 1986 | 3301333 | |
| [testing of the footpad technic for the cultivation of mycobacterium leprae in different lines of mice populations in cuba]. | 1986 | 3299546 | |
| [genetic component of the immune response to mycobacterium leprae in healthy subjects]. | 1986 | 3306256 | |
| vaccination of borderline tuberculoid leprosy patients with bcg plus killed mycobacterium leprae. | 1986 | 3316142 | |
| the experimental inoculation with mycobacterium leprae in the congenitally asplenic mouse. | 1986 | 3316143 | |
| the nude mouse as an experimental lepromatous leprosy model (continued): the lepromatoid lesions in mystacial vibrissae located site of injection. | 1986 | 3316144 | |
| survey of primary dapsone-resistant leprosy. | 1986 | 3321049 | |
| an attempt to induce a histological enl symptom in m. leprae infected nude mice. | 1986 | 3323158 | |
| a cooperative taxonomic study of mycobacteria isolated from armadillos infected with mycobacterium leprae. | seventeen strains of mycobacteria, recovered from six armadillos experimentally infected with mycobacterium leprae, were examined in ten different laboratories. this collaborative study included use of conventional bacteriological tests, lipid analyses, determination of mycobactins and peptidoglycans, characterization by py-ms, and immunological, metabolic, pathological and dna studies. these armadillo-derived mycobacteria (adm) formed five homogeneous groups (numbered adm 1 to 5) on the basis o ... | 1986 | 3305778 |
| pyrolysis gas chromatography-mass spectrometry of mycobacterial mycolic acid methyl esters and its application to the identification of mycobacterium leprae. | pyrolysis gas chromatography-mass spectrometry of methyl mycolates from 32 species of mycobacteria, including mycobacterium leprae, was carried out. the mycobacteria could be classified into four groups in respect of the fatty acid ester patterns detected within the range c20 to c26. the applicability of this pyrolysis-gas chromatographic method for identifying m. leprae is discussed. | 1986 | 3309136 |
| immunology of leprosy: new findings, future perspectives. | 1986 | 3334152 | |
| a preliminary study on serological activity of phenolic glycolipid and its application in diagnosis of leprosy. | 1986 | 3422995 | |
| interleukin 1--a possible mediator of neural fibrosis in leprosy. | neural fibrosis in leprosy, the disease caused by the obligate intracellular bacterium, mycobacterium leprae, is closely related to the cell-mediated immune response to this microorganism. the fibrosis appears to be due to soluble mediators released by the immune effector cells. interleukin 1, a macrophage product that stimulates fibroblast migration, proliferation and synthetic activity, possesses the requisite properties to qualify for such a mediator. | 1986 | 3488493 |
| accessory cell function of cells isolated from mycobacterium leprae-induced granulomas. | the large cells from mycobacterium leprae-induced granulomas in guinea pig lymph nodes were separated by percoll discontinuous density gradient centrifugation and on a fluorescence-activated cell sorter (facs) using cross-reacting monoclonal antibody to human mhc class ii antigens. large percoll-separated cells (83% class ii antigen positive and 52% macrophage-specific antigen positive) and facs-separated cells are able to act as antigen-presenting cells for t-cell proliferation to ppd. in previ ... | 1986 | 3492279 |
| abilities of human oligodendroglial cells and mouse schwann cells to phagocytose mycobacterium leprae and other mycobacteria. | human oligodendroglial kg-1-c cells derived from human cerebral mixed glioma and mouse schwann cells derived from dorsal root ganglion were studied with respect to their abilities to phagocytose various mycobacteria, especially mycobacterium leprae, and other microorganisms. kg-1-c cells phagocytosed m. leprae at a markedly higher rate than balb/3t3, bhk 21, hela s3, mks-a tu-7, xc, tsv-5, n-18, and schwann cells but at a lower rate than peritoneal macrophages. schwann cells also exhibited subst ... | 1986 | 3510165 |
| mechanisms of phagocytosis of mycobacterium leprae and other mycobacteria by human oligodendroglial cells. | the mechanisms by which human oligodendroglial cells, kg-1-c cells, phagocytose mycobacteria, especially mycobacterium leprae, were studied. the ability of glial cells to phagocytose m. leprae was inhibited by azide, dinitrophenol (inhibitors of oxidative phosphorylation), and iodoacetamide but not fluoride (both are inhibitors of glycolysis). thus, the energy metabolism dependency is somewhat different from that of peritoneal macrophages and polymorphonuclear leukocytes, the phagocytic capaciti ... | 1986 | 3510167 |
| human t-cell clones recognize a major m. leprae protein antigen expressed in e. coli. | leprosy is a chronic infectious disease caused by mycobacterium leprae. as with other intracellular parasites, protective immunity is dependent on t cells and cell-mediated immunity. in animal models, immunization with killed armadillo-derived m. leprae elicits strong t-cell responses, delayed-type hypersensitivity and protection against viable challenge. we have recently shown that killed m. leprae can induce delayed-type hypersensitivity in healthy human volunteers. identification of the m. le ... | 1986 | 3510397 |
| quantitation of the phenolic glycolipid of mycobacterium leprae and relevance to glycolipid antigenemia in leprosy. | chemical and immunologic procedures have been developed for quantitation, in the body fluids of patients with leprosy, of phenolic glycolipid i, the major specific antigen of the leprosy bacillus. serum samples were extracted with chcl3/ch3oh and fractionated on columns of silicic acid. thin-layer chromatography with a sensitivity of about 500 ng allowed detection of the glycolipid in untreated lepromatous and borderline patients, and high-pressure liquid chromatography gave a quantitation of 0. ... | 1986 | 3512732 |
| interleukin-1 released by blood-monocyte-derived macrophages from patients with leprosy. | in highly purified blood-monocyte-derived macrophages collected from patients with leprosy and from healthy individuals and cultured in vitro with mycobacterial antigens such as mycobacterium bovis bcg or mycobacterium leprae, we nonspecifically induced the synthesis of interleukin-1. normally, all supernatants from cultured macrophages of all subjects tested produced similar amounts of interleukin-1. however, only in patients with lepromatous leprosy, m. leprae, but not bcg, induced high-level ... | 1986 | 3514458 |
| the immunobiology of leprosy. | leprosy, a chronic infectious disease of man, is caused by the obligate intracellular bacterium m. leprae. infection with m. leprae affects the peripheral nerves and the dermis, causing an accumulation of macrophages and other immune cells at the infected sites. host resistance to the bacterium determines the extent of local inflammatory reactions and its resulting damage to the affected tissues. in lepromatous disease little if any cellular immunity develops. bacterial multiplication is uncontr ... | 1986 | 3516911 |
| the number of mycobacterium leprae in the pretreatment biopsy-specimen does not determine the rate of response of patients with lepromatous leprosy to chemotherapy with acedapsone. | 1986 | 3517535 | |
| secondary dapsone-resistant leprosy in brazil: a preliminary report. | 1986 | 3517536 | |
| m. leprae phagocytosis and its association with membrane changes in macrophages from leprosy patients. | abnormal phagocytosis of mycobacterium leprae by macrophages of lepromatous patients was demonstrated under various conditions. the largest proportion of macrophages with an excessive bacterial load belonged to the lepromatous group of patients. lepromatous macrophages treated with cytochalasin b, an inhibitor of phagocytosis, exhibited a significantly lower degree of ingestion of heat-killed organisms whereas uptake of 'viable' organisms was not affected to the same extent. regulation of phagoc ... | 1986 | 3517765 |
| chemical synthesis of disaccharides of the specific phenolic glycolipid antigens from mycobacterium leprae and of related sugars. | o-(3,6-di-o-methyl-beta-d-glucopyranosyl)-(1----4)-2,3,-di-o-methyl-l -rhamnopyranose, which is the nonreducing disaccharide of the haptenic trisaccharide of the mycobacterium leprae-specific, phenolic glycolipid i, o-(6-o-methyl-beta-d-glucopyranosyl)-(1----4)-2,3-di-o-methyl-l-rhamn opyranose, the nonreducing end of the specific, phenolic glycolipid iii, and the nonhaptenic o-beta-(d-glucopyranosyl)-(1----4)-2,3-di-o-methyl-l-rhamnopyranose++ +, were synthesized in relatively good yield from 3 ... | 1986 | 3518927 |
| preliminary study of a mycobacterium leprae bacterin vaccine in a human volunteer population in a nonendemic area. | seventeen volunteers who had never resided in areas with significant transmission of leprosy (hansen's disease) were inoculated intradermally with 1.5 x 10(8) killed, purified armadillo-derived mycobacterium leprae in a standardized preparation being tested for possible vaccine use. the peak of local skin responses, consisting of induration with or without ulceration similar to the post-lepromin mitsuda reaction, occurred between the 21st and 28th days after injection. the maximum mean induratio ... | 1986 | 3519481 |
| [drug resistance of mycobacterium leprae]. | 1986 | 3551466 | |
| [vaccination against leprosy]. | 1986 | 3551467 | |
| classification of nerves is modified by the delayed recognition of mycobacterium leprae. | biopsies of 42 concurrent nerve and skin lesions across the spectrum of leprosy were classified and compared histologically and bacteriologically. observations were made as follows: a) the bacterial load was higher in nerve than in skin lesions of the same histological classification, and it was higher in nerve than in concurrent skin lesions irrespective of classification, although not at the lepromatous pole. b) there was some discrepancy between the histological classification of nerve and sk ... | 1986 | 3546549 |