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mycothiol protects corynebacterium glutamicum against acid stress via maintaining intracellular ph homeostasis, scavenging ros, and s-mycothiolating mete.mycothiol (msh) plays a major role in protecting cells against oxidative stress and detoxification from a broad range of exogenous toxic agents. in the present study, we reveal that intracellular msh contributes significantly to the adaptation to acidic conditions in the model organism corynebacterium glutamicum. we present evidence that msh confers c. glutamicum with the ability to adapt to acidic conditions by maintaining phi homeostasis, scavenging reactive oxygen species (ros), and protectin ...201627250661
structural characterization of heme environmental mutants of cghmut that shuttles heme molecules to heme transporters.corynebacteria contain a heme uptake system encoded in hmutuv genes, in which hmut protein acts as a heme binding protein to transport heme to the cognate transporter hmuuv. the crystal structure of hmut from corynebacterium glutamicum (cghmut) reveals that heme is accommodated in the central cleft with his141 and tyr240 as the axial ligands and that tyr240 forms a hydrogen bond with arg242. in this work, the crystal structures of h141a, y240a, and r242a mutants were determined to understand the ...201627240352
cathodes enhance corynebacterium glutamicum growth with nitrate and promote acetate and formate production.the industrially important corynebacterium glutamicum can only incompletely reduce nitrate into nitrite which then accumulates and inhibits growth. herein we report that cathodes can resolve this problem and enhance glucose fermentation and growth by promoting nitrite reduction. cell growth was inhibited at relatively high potentials but was significant when potentials were more reductive (-1.20v with anthraquinone-2-sulfonate as redox mediator or -1.25v vs. ag/agcl). under these conditions, glu ...201627235972
overexpression of ppc and lysc to improve the production of 4-hydroxyisoleucine and its precursor l-isoleucine in recombinant corynebacterium glutamicum ssp. lactofermentum.4-hydroxyisoleucine (4-hil) exhibits unique insulinotropic and insulin-sensitizing activities and is an attractive candidate for the treatment of type ii and type i diabetes. in our previous study, l-isoleucine dioxygenase gene (ido) was cloned and overexpressed in an l-isoleucine-producing strain, corynebacterium glutamicum ssp. lactofermentum sn01, and 4-hil was produced from the endogenous l-isoleucine (ile). in this study, ppc and lysc were co-expressed with ido to increase the supply of ile ...201627178798
effect of corynebacterium glutamicum on livestock material burial treatment.in recent years, foot-and-mouth disease has occurred in all parts of the world. the animals with the disease are buried in the ground; therefore, their concentration could affect ground or groundwater. moreover, the complete degradation of carcasses is not a certainty, and their disposal is important to prevent humans, livestock, and the environment from being affected with the disease. the treatment of corynebacterium glutamicum is a feasible method to reduce the risk of carcass decomposition a ...201627160580
recent advances in amino acid production by microbial cells.amino acids have been utilized for the production of foods, animal feeds and pharmaceuticals. after the discovery of the glutamic acid-producing bacterium corynebacterium glutamicum by japanese researchers, the production of amino acids, which are primary metabolites, has been achieved using various microbial cells as hosts. recently, metabolic engineering studies on the rational design of amino acid-producing microbial cells have been successfully conducted. moreover, the technology of systems ...201627151315
transcriptional regulation of the β-type carbonic anhydrase gene bca by rama in corynebacterium glutamicum.carbonic anhydrase catalyzes the reversible hydration of carbon dioxide to bicarbonate and maintains the balance of co2/hco3- in the intracellular environment, specifically for carboxylation/decarboxylation reactions. in corynebacterium glutamicum, two putative genes, namely the bca (cg2954) and gca (cg0155) genes, coding for β-type and γ-type carbonic anhydrase, respectively, have been identified. we here analyze the transcriptional organization of these genes. the transcriptional start site (t ...201627119954
the pupylation machinery is involved in iron homeostasis by targeting the iron storage protein ferritin.the balance of sufficient iron supply and avoidance of iron toxicity by iron homeostasis is a prerequisite for cellular metabolism and growth. here we provide evidence that, in actinobacteria, pupylation plays a crucial role in this process. pupylation is a posttranslational modification in which the prokaryotic ubiquitin-like protein pup is covalently attached to a lysine residue in target proteins, thus resembling ubiquitination in eukaryotes. pupylated proteins are recognized and unfolded by ...201627078093
enhanced succinic acid production in corynebacterium glutamicum with increasing the available nadh supply and glucose consumption rate by decreasing h(+)-atpase activity.to enhance succinic acid production in corynebacterium glutamicum by increasing the supply of nadh and the rate of glucose consumption by decreasing h(+)-atpase activity.201627053082
mycothiol peroxidase mpx protects corynebacterium glutamicum against acid stress by scavenging ros.to investigate mycothiol peroxidase (mpx) of corynebacterium glutamicum that is a novel cysgpx family peroxidase using both the mycoredoxin and thioredoxin reducing systems as proton donors for peroxide detoxification and may be involved in the relief of acid stress.201627053080
characterization of aspartate kinase and homoserine dehydrogenase from corynebacterium glutamicum iwj001 and systematic investigation of l-isoleucine biosynthesis.previously we have characterized a threonine dehydratase mutant td(f383v) (encoded by ilva1) and an acetohydroxy acid synthase mutant ahas(p176s, d426e, l575w) (encoded by ilvbn1) in corynebacterium glutamicum iwj001, one of the best l-isoleucine producing strains. here, we further characterized an aspartate kinase mutant ak(a279t) (encoded by lysc1) and a homoserine dehydrogenase mutant hd(g378s) (encoded by hom1) in iwj001, and analyzed the consequences of all these mutant enzymes on amino aci ...201627033538
enhancing pentose phosphate pathway in corynebacterium glutamicum to improve l-isoleucine production.three genes, gnd, pgl, and fbp, relevant to the pentose phosphate pathway (ppp) were overexpressed in corynebacterium glutamicum iwj001, leading to increase of l-isoleucine production. the transcriptional levels of gnd, pgl, and fbp significantly increased in iwj001/pdxw-8-gnd-fbp-pgl. compared with the control strain iwj001/pdxw-8, intracellular nadph/nadp(+) ratios in iwj001/pdxw-8-gnd and iwj001/pdxw-8-gnd-fbp cells grown for 36 h increased threefold and fourfold, respectively, indicating tha ...201627010514
spie interacts with corynebacterium glutamicum whce and is involved in heat and oxidative stress responses.the gene whce in corynebacterium glutamicum positively responds to oxidative and heat stress. to search for proteins that interact with whce, we employed a two-hybrid system with whce as the bait. sequencing analysis of the isolated clones revealed peptide sequences, one of which showed high sequence identity to a hydrophobe/amphiphile efflux-1 family transporter encoded by ncgl1497. the interaction of the ncgl1497-encoded protein with whce in vivo was verified using reporter gene expression by ...201626996627
detoxification of acidic biorefinery waste liquor for production of high value amino acid.the current study evaluates the detoxification of acid pretreatment liquor (apl) using adsorbent (ads 400 & ads 800) or ion-exchange (a-27mp & a-72mp) resins and its potential for amino acid production. the apl is generated as a by-product from the pretreatment of lignocellulosic biomass and is rich monomeric sugars as well as sugar degradation products (fermentation inhibitors) such as furfural and hydroxymethyl furfural (hmf). of the four resins compared, ads 800 removed approximately 85% and ...201626996259
pyruvate kinase deletion as an effective phenotype to enhance lysine production in corynebacterium glutamicum atcc13032: redirecting the carbon flow to a precursor metabolite.various attempts have been made to enhance lysine production in corynebacterium glutamicum. pyruvate kinase (pyk) defect is one of the strategies used to enhance the supply of oxaloacetic acid (oaa), a precursor metabolite for lysine biosynthesis. however, inconsistent effects of this mutation have been reported: positive effects of pyk defect in mutants having phosphoenolpyruvate carboxylase (pepc) desensitized to feedback inhibition by aspartic acid, while negative effects in simple pyk gene ( ...201626983943
efficient production of α-ketoglutarate in the gdh deleted corynebacterium glutamicum by novel double-phase ph and biotin control strategy.production of l-glutamate using a biotin-deficient strain of corynebacterium glutamicum has a long history. the process is achieved by controlling biotin at suboptimal dose in the initial fermentation medium, meanwhile feeding nh4oh to adjust ph so that α-ketoglutarate (α-kg) can be converted to l-glutamate. in this study, we deleted glutamate dehydrogenase (gdh1 and gdh2) of c. glutamicum gkg-047, an l-glutamate overproducing strain, to produce α-kg that is the direct precursor of l-glutamate. ...201626946492
enhancement of 1,5-diaminopentane production in a recombinant strain of corynebacterium glutamicum by tween 40 addition. 201626923131
mutagenesis for improvement of activity and thermostability of amylomaltase from corynebacterium glutamicum.this work aims to improve thermostability of amylomaltase from a mesophilic corynebacterium glutamicum (cgam) by random and site-directed mutagenesis. from error prone pcr, a mutated cgam with higher thermostability at 50 °c compared to the wild-type was selected and sequenced. the result showed that the mutant contains a single mutation of a406v. site-directed mutagenesis was then performed to construct a406v and a406l. both mutated cgams showed higher intermolecular transglucosylation activity ...201626875536
optimization of large-ring cyclodextrin production from starch by amylomaltase from corynebacterium glutamicum and effect of organic solvent on product size.to increase yield of starch conversion to large-ring cyclodextrins (lr-cds) by amylomaltase from corynebacterium glutamicum (cgam).201626849173
the actinobacterium corynebacterium glutamicum, an industrial workhorse.starting as a glutamate producer, corynebacterium glutamicum has played a variety of roles in the industrial production of amino acids, one of the most important areas of white biotechnology. from shortly after its genome information became available, c. glutamicum has been applied in various production processes for value-added chemicals, fuels, and polymers, as a key organism in industrial biotechnology alongside the surprising progress in systems biology and metabolic engineering. in addition ...201626838341
corynebacterium glutamicum metabolic engineering with crispr interference (crispri).corynebacterium glutamicum is an important organism for the industrial production of amino acids. metabolic pathways in this organism are usually engineered by conventional methods such as homologous recombination, which depends on rare double-crossover events. to facilitate the mapping of gene expression levels to metabolic outputs, we applied crispr interference (crispri) technology using deactivated cas9 (dcas9) to repress genes in c. glutamicum. we then determined the effects of target repre ...201626829286
characterization of a unique pathway for 4-cresol catabolism initiated by phosphorylation in corynebacterium glutamicum.4-cresol is not only a significant synthetic intermediate for production of many aromatic chemicals, but also a priority environmental pollutant because of its toxicity to higher organisms. in our previous studies, a gene cluster implicated to be involved in 4-cresol catabolism, crecdefghir, was identified in corynebacterium glutamicum and partially characterized in vivo. in this work, we report on the discovery of a novel 4-cresol biodegradation pathway that employs phosphorylated intermediates ...201626817843
modular optimization of a hemicellulose-utilizing pathway in corynebacterium glutamicum for consolidated bioprocessing of hemicellulosic biomass.hemicellulose, which is the second most abundant polysaccharide in nature after cellulose, has the potential to become a major feedstock for microbial fermentation to produce various biofuels and chemicals. to utilize hemicellulose economically, it is necessary to develop a consolidated bioprocess (cbp), in which all processes from biomass degradation to the production of target products occur in a single bioreactor. here, we report a modularly engineered corynebacterium glutamicum strain suitab ...201626808593
adaptive evolution and metabolic engineering of a cellobiose- and xylose- negative corynebacterium glutamicum that co-utilizes cellobiose and xylose.an efficient microbial cell factory requires a microorganism that can utilize a broad range of substrates to economically produce value-added chemicals and fuels. the industrially important bacterium corynebacterium glutamicum has been studied to broaden substrate utilizations for lignocellulose-derived sugars. however, c. glutamicum atcc 13032 is incapable of pts-dependent utilization of cellobiose because it has missing genes annotated to β-glucosidases (bg) and cellobiose-specific pts permeas ...201626801253
the extracytoplasmic function σ factor σ(c) regulates expression of a branched quinol oxidation pathway in corynebacterium glutamicum.bacteria modify their expression of different terminal oxidases in response to oxygen availability. corynebacterium glutamicum, a facultative anaerobic bacterium of the phylum actinobacteria, possesses aa3 -type cytochrome c oxidase and cytochrome bd-type quinol oxidase, the latter of which is induced by oxygen limitation. we report that an extracytoplasmic function σ factor, σ(c) , is responsible for the regulation of this process. chromatin immunoprecipitation with microarray analysis detected ...201626789738
complete genome sequence of corynebacterium glutamicum cp, a chinese l-leucine producing strain.here, we report the complete genome sequence of corynebacterium glutamicum cp, an industrial l-leucine producing strain in china. the whole genome consists of a circular chromosome and a plasmid. the comparative genomics analysis shows that there are many mutations in the key enzyme coding genes relevant to l-leucine biosynthesis compared to c. glutamicum atcc 13032.201626784991
development of a potential stationary-phase specific gene expression system by engineering of sigb-dependent cg3141 promoter in corynebacterium glutamicum.corynebacterium glutamicum is a non-pathogenic, non-sporulating gram-positive soil bacterium that has been used for the industrial production of various proteins and chemicals. to achieve enhanced and economical production of target molecules, the development of strong auto-inducible promoters is desired, which can be activated without expensive inducers and has significant advantages for industrial-scale use. here, we developed a stationary-phase gene expression system by engineering a sigma fa ...201626782746
metabolic engineering of corynebacterium glutamicum atcc13032 to produce s-adenosyl-l-methionine.as an important biological methyl group donor, s-adenosyl-l-methionine is used as nutritional supplement or drug for various diseases, but bacterial strains that can efficiently produce s-adenosyl-l-methionine are not available. in this study, corynebacterium glutamicum strain hw104 which can accumulate s-adenosyl-l-methionine was constructed from c. glutamicum atcc13032 by deleting four genes thrb, metb, mcbr and ncgl2640, and six genes metk, vgb, lysc(m), hom(m), metx and mety were overexpress ...201626777246
structure of amtr, the global nitrogen regulator of corynebacterium glutamicum, in free and dna-bound forms.corynebacterium glutamicum is a bacterium used for industrial amino acid production, and understanding its metabolic pathway regulation is of high biotechnological interest. here, we report crystal structures of amtr, the global nitrogen regulator of c. glutamicum, in apo (2.25-å and 2.65-å resolution) and dna-bound (3-å resolution) forms. these structures reveal an all-α homodimeric tetr family regulator composed of a helix-turn-helix-hosting n-terminal dna-binding domain and a c-terminal dimer ...201626744254
pathway construction in corynebacterium glutamicum and strain engineering to produce rare sugars from glycerol.rare sugars are valuable natural products widely used in pharmaceutical and food industries. in this study, we expected to synthesize rare ketoses from abundant glycerol using dihydroxyacetone phosphate (dhap)-dependent aldolases. first, a new glycerol assimilation pathway was constructed to synthesize dhap. the enzymes which convert glycerol to 3-hydroxypropionaldehyde and l-glyceraldehyde were selected, and their corresponding aldehyde synthesis pathways were constructed in vivo. four aldol pa ...201627998065
metabolic engineering of cyanobacteria for the photosynthetic production of succinate.succinate is an important commodity chemical currently used in the food, pharmaceutical, and polymer industries. it can also be chemically converted into other major industrial chemicals such as 1,4-butanediol, butadiene, and tetrahydrofuran. here we metabolically engineered a model cyanobacterium synechococcus elongatus pcc 7942 to photosynthetically produce succinate. we expressed the genes encoding for α-ketoglutarate decarboxylase and succinate semialdehyde dehydrogenase in s. elongatus pcc ...201627989804
transcriptome and multivariable data analysis of corynebacterium glutamicum under different dissolved oxygen conditions in bioreactors.dissolved oxygen (do) is an important factor in the fermentation process of corynebacterium glutamicum, which is a widely used aerobic microbe in bio-industry. herein, we described rna-seq for c. glutamicum under different do levels (50%, 30% and 0%) in 5 l bioreactors. multivariate data analysis (mvda) models were used to analyze the rna-seq and metabolism data to investigate the global effect of do on the transcriptional distinction of the substance and energy metabolism of c. glutamicum. the ...201627907077
a novel pyruvate kinase and its application in lactic acid production under oxygen deprivation in corynebacterium glutamicum.pyruvate kinase (pyk) catalyzes the generation of pyruvate and atp in glycolysis and functions as a key switch in the regulation of carbon flux distribution. both the substrates and products of pyk are involved in the tricarboxylic acid cycle, anaplerosis and energy anabolism, which places pyk at a primary metabolic intersection. pyks are highly conserved in most bacteria and lower eukaryotes. corynebacterium glutamicum is an industrial workhorse for the production of various amino acids and org ...201627852252
corynebacterium glutamicum for sustainable bioproduction: from metabolic physiology to systems metabolic engineering.since its discovery 60 years ago, corynebacterium glutamicum has evolved into a workhorse for industrial biotechnology. traditionally well known for its remarkable capacity to produce amino acids, this gram-positive soil bacterium, has become a flexible, efficient production platform for various bulk and fine chemicals, materials, and biofuels. the central turnstile of all these achievements is our excellent understanding of its metabolism and physiology. this knowledge base, together with innov ...201627872959
metabolic engineering of corynebacterium glutamicum for shikimate overproduction by growth-arrested cell reaction.corynebacterium glutamicum with the ability to simultaneously utilize glucose/pentose mixed sugars was metabolically engineered to overproduce shikimate, a valuable hydroaromatic compound used as a starting material for the synthesis of the anti-influenza drug oseltamivir. to achieve this, the shikimate kinase and other potential metabolic activities for the consumption of shikimate and its precursor dehydroshikimate were inactivated. carbon flux toward shikimate synthesis was enhanced by overex ...201627553883
construction of a corynebacterium glutamicum platform strain for the production of stilbenes and (2s)-flavanones.corynebacterium glutamicum is an important organism in industrial biotechnology for the microbial production of bulk chemicals, in particular amino acids. however, until now activity of a complex catabolic network for the degradation of aromatic compounds averted application of c. glutamicum as production host for aromatic compounds of pharmaceutical or biotechnological interest. in the course of the construction of a suitable c. glutamicum platform strain for plant polyphenol production, four g ...201627288926
global transcriptomic analysis of the response of corynebacterium glutamicum to vanillin.lignocellulosic biomass is an abundant and renewable resource for biofuels and bio-based chemicals. vanillin is one of the major phenolic inhibitors in biomass production using lignocellulose. to assess the response of corynebacterium glutamicum to vanillin stress, we performed a global transcriptional response analysis. the transcriptional data showed that the vanillin stress not only affected the genes involved in degradation of vanillin, but also differentially regulated several genes related ...201627760214
glutamine metabolism of corynebacterium glutamicum: role of the glutaminase glsk.corynebacterium glutamicum is able to metabolize different nitrogen and carbon sources. in standard minimal media, ammonium and urea typically serve as nitrogen source and glucose or sucrose as carbon and energy source; however, amino acids might also play a role as nitrogen, carbon and energy source. in this study, the function of the putative glutaminase glsk was investigated. a glsk deletion strain showed impaired growth with l-glutamine as carbon and energy source, while growth was improved ...201627754855
production of 2-methyl-1-butanol and 3-methyl-1-butanol in engineered corynebacterium glutamicum.the pentanol isomers 2-methyl-1-butanol and 3-methyl-1-butanol represent commercially interesting alcohols due to their potential application as biofuels. for a sustainable microbial production of these compounds, corynebacterium glutamicum was engineered for producing 2-methyl-1-butanol and 3-methyl-1-butanol via the ehrlich pathway from 2-keto-3-methylvalerate and 2-ketoisocaproate, respectively. in addition to an already available 2-ketoisocaproate producer, a 2-keto-3-methylvalerate accumula ...201627746323
increased glucose utilization and cell growth of corynebacterium glutamicum by modifying the glucose-specific phosphotransferase system (pts(glc)) genes.the phosphoenolpyruvate:glucose phosphotransferase system (pts(glc)) is the major pathway of glucose uptake in corynebacterium glutamicum. this study investigated glucose consumption rate, cell growth, and metabolite changes resulting from modification of pts(glc). the classical l-lysine producer c. glutamicum xq-8 exhibited low glucose consumption, cell growth, and l-lysine production rates, whereas these parameters were significantly increased during cultivating on glucose plus maltose, throug ...201627718589
ciprofloxacin triggered glutamate production by corynebacterium glutamicum.corynebacterium glutamicum is a well-studied bacterium which naturally overproduces glutamate when induced by an elicitor. glutamate production is accompanied by decreased 2-oxoglutatate dehydrogenase activity. elicitors of glutamate production by c. glutamicum analyzed to molecular detail target the cell envelope.201627717325
attenuating l-lysine production by deletion of ddh and lyse and their effect on l-threonine and l-isoleucine production in corynebacterium glutamicum.the fermentative production of l-threonine and l-isoleucine with corynebacterium glutamicum is usually accompanied by the by-production of l-lysine, which shares partial biosynthesis pathway with l-threonine and l-isoleucine. since the direct precursor for l-lysine synthesis, diaminopimelate, is a component of peptidoglycan and thus essential for cell wall synthesis, reducing l-lysine by-production could be troublesome. here, a basal strain with eliminated l-lysine production was constructed fro ...201627702487
a toolbox of genetically encoded fret-based biosensors for rapid l-lysine analysis.background: the fast development of microbial production strains for basic and fine chemicals is increasingly carried out in small scale cultivation systems to allow for higher throughput. such parallelized systems create a need for new rapid online detection systems to quantify the respective target compound. in this regard, biosensors, especially genetically encoded förster resonance energy transfer (fret)-based biosensors, offer tremendous opportunities. as a proof-of-concept, we have created ...201627690044
transcriptomic analysis for elucidating the physiological effects of 5-aminolevulinic acid accumulation on corynebacterium glutamicum.5-aminolevulinic acid (ala), the committed intermediate of the heme biosynthetic pathway, attracts close attention among researchers because of its potential applications to cancer treatment and agriculture. overexpression of heterologous hema and heml, which encode glutamyl-trna reductase and glutamate-1-semialdehyde aminotransferase, respectively, in corynebacterium glutamicum produces ala, although whether ala accumulation causes unintended effects on the host is unknown. here we used an inte ...201627664748
elucidation of the regulatory role of the fructose operon reveals a novel target for enhancing the nadph supply in corynebacterium glutamicum.the performance of corynebacterium glutamicum cell factories producing compounds which rely heavily on nadph has been reported to depend on the sugar being metabolized. while some aspects of this phenomenon have been elucidated, there are still many unresolved questions as to how sugar metabolism is linked to redox and to the general metabolism. we here provide new insights into the regulation of the metabolism of this important platform organism by systematically characterizing mutants carrying ...201627553884
light-controlled cell factories: employing photocaged isopropyl-β-d-thiogalactopyranoside for light-mediated optimization of lac promoter-based gene expression and (+)-valencene biosynthesis in corynebacterium glutamicum.precise control of microbial gene expression resulting in a defined, fast, and homogeneous response is of utmost importance for synthetic bio(techno)logical applications. however, even broadly applied biotechnological workhorses, such as corynebacterium glutamicum, for which induction of recombinant gene expression commonly relies on the addition of appropriate inducer molecules, perform moderately in this respect. light offers an alternative to accurately control gene expression, as it allows f ...201627520809
roles of n287 in catalysis and product formation of amylomaltase from corynebacterium glutamicum.amylomaltase catalyzes intermolecular and intramolecular transglucosylation reactions to form linear and cyclic oligosaccharides, respectively. the aim of this work is to investigate the structure-function relationship of amylomaltase from a mesophilic corynebacterium glutamicum (cgam). site-directed mutagenesis was performed to substitute tyr for asn287 (n287y) to determine its role in controlling amylomaltase activity and product formation. expression of the wild-type (wt) and n287y was achiev ...201627507216
silencing of cryptic prophages in corynebacterium glutamicum.dna of viral origin represents a ubiquitous element of bacterial genomes. its integration into host regulatory circuits is a pivotal driver of microbial evolution but requires the stringent regulation of phage gene activity. in this study, we describe the nucleoid-associated protein cgps, which represents an essential protein functioning as a xenogeneic silencer in the gram-positive corynebacterium glutamicum cgps is encoded by the cryptic prophage cgp3 of the c. glutamicum strain atcc 13032 and ...201627492287
improvement of the intracellular environment for enhancing l-arginine production of corynebacterium glutamicum by inactivation of h2o2-forming flavin reductases and optimization of atp supply.l-arginine, a semi essential amino acid, is an important amino acid in food flavoring and pharmaceutical industries. its production by microbial fermentation is gaining more and more attention. in previous work, we obtained a new l-arginine producing corynebacterium crenatum (subspecies of corynebacterium glutamicum) through mutation breeding. in this work, we enhanced l-arginine production through improvement of the intracellular environment. first, two nad(p)h-dependent h2o2-forming flavin red ...201627474351
the obligate respiratory supercomplex from actinobacteria.actinobacteria are closely linked to human life as industrial producers of bioactive molecules and as human pathogens. respiratory cytochrome bcc complex and cytochrome aa3 oxidase are key components of their aerobic energy metabolism. they form a supercomplex in the actinobacterial species corynebacterium glutamicum. with comprehensive bioinformatics and phylogenetic analysis we show that genes for cyt bcc-aa3 supercomplex are characteristic for actinobacteria (actinobacteria and acidimicrobiia ...201627472998
erratum to: the actinobacterium corynebacterium glutamicum, an industrial workhorse.this erratum is being published to correct the 3rd author's name of above manuscript by lee et al. that was published in journal of microbiology and biotechnology (2016, 26: 807-822). the 3rd author name(eungsoo kim) should appear as 'eung-soo kim'.201627452344
overexpression of mycothiol disulfide reductase enhances corynebacterium glutamicum robustness by modulating cellular redox homeostasis and antioxidant proteins under oxidative stress.mycothiol (msh) is the dominant low-molecular-weight thiol (lmwt) unique to high-(g+c)-content gram-positive actinobacteria, such as corynebacterium glutamicum, and is oxidised into its disulfide form mycothiol disulfide (mssm) under oxidative conditions. mycothiol disulfide reductase (mtr), an nadph-dependent enzyme, reduces mssm to msh, thus maintaining intracellular redox homeostasis. in this study, a recombinant plasmid was constructed to overexpress mtr in c. glutamicum using the expression ...201627383057
a method for simultaneous gene overexpression and inactivation in the corynebacterium glutamicum genome.the gene integration method is an important tool to stably express desirable genes in bacteria. to avoid heavy workload and cost, we constructed a rapid and efficient method for genome modification. this method depended on a mobilizable plasmid, which contains a p tac promoter, an introduced multiple cloning site (imcs), and rrnbt1t2 terminator. briefly, the mobilizable plasmid pk18-mbpmt with the p tac-imcs-rrnbt1t2 cartridge derived from pk18mobsacb was prepared to directly integrate hetero-/h ...201627377799
biotypes analysis of corynebacterium glutamicum growing in dicarboxylic acids demonstrates the existence of industrially-relevant intra-species variations.production enhancement of industrial microbial products or strains has been traditionally tackled by mutagenesis with chemical methods, irradiation or genetic manipulation. however, the final yield increase must go hand in hand with the resistance increasing against the usual inherent toxicity of the final products. few studies have been carried out on resistance improvement and even fewer on the initial selection of naturally-generated biotypes, which could decrease the artificial mutagenesis. ...201627371347
crystal structure of amylomaltase from corynebacterium glutamicum.amylomaltase is an essential enzyme in maltose utilization and maltodextrin metabolism, and it has been industrially used for the production of cyclodextrin and modification of starch. we determined the crystal structure of amylomaltase from corynebacterium glutamicum (cgam) at a resolution of 1.7 å. although cgam forms a dimer without nacl, it exists as a monomer in physiological concentration of nacl. cgam is composed of n- and c-terminal domains, which can be further divided into two and four ...201627366969
the small 6c rna of corynebacterium glutamicum is involved in the sos response.the 6c rna family is a class of small rnas highly conserved in actinobacteria, including the genera mycobacterium, streptomyces and corynebacterium whose physiological function has not yet been elucidated. we found that strong transcription of the cgb_03605 gene, which encodes 6c rna in c. glutamicum, was driven by the siga- and sigb-dependent promoter pcgb_03605. 6c rna was detected at high level during exponential growth phase (180 to 240 molcules per cell) which even increased at the entry of ...201627362471
a novel acee mutation leading to a better growth profile and a higher l-serine production in a high-yield l-serine-producing corynebacterium glutamicum strain.a comparative genomic analysis was performed to study the genetic variations between the l-serine-producing strain corynebacterium glutamicum syps-062 and the mutant strain syps-062-33a, which was derived from syps-062 by random mutagenesis with enhanced l-serine production. some variant genes between the two strains were reversely mutated or deleted in the genome of syps-062-33a to verify the influences of the gene mutations introduced by random mutagenesis. it was found that a his-594 → tyr mu ...201627344574
systems pathway engineering of corynebacterium crenatum for improved l-arginine production.l-arginine is an important amino acid in food and pharmaceutical industries. until now, the main production method of l-arginine in china is the highly polluting keratin acid hydrolysis. the industrial level l-arginine production by microbial fermentation has become an important task. in previous work, we obtained a new l-arginine producing corynebacterium crenatum (subspecies of corynebacterium glutamicum) through screening and mutation breeding. in this work, we performed systems pathway engin ...201627338253
3-methyl-1-butanol biosynthesis in an engineered corynebacterium glutamicum.biofuel offers a promising solution to the adverse environmental problems and depletion in reserves of fossil fuels. higher alcohols including 3-methyl-1-butanol were paid much more attention as fuel substitute in recent years, due to its similar properties to gasoline. in the present work, 3-methyl-1-butanol production in engineered corynebacterium glutamicum was studied. α-ketoisovalerate decarboxylase gene (kivd) from lactococcus lactis combined with alcohol dehydrogenase gene (adh2, adha, an ...201626961908
structural insight into dihydrodipicolinate reductase from corybebacterium glutamicum for lysine biosynthesis.dihydrodipicolinate reductase is an enzyme that converts dihydrodipicolinate to tetrahydrodipicolinate using an nad(p)h cofactor in l-lysine biosynthesis. to increase the understanding of the molecular mechanisms of lysine biosynthesis, we determined the crystal structure of dihydrodipicolinate reductase from corynebacterium glutamicum (cgdapb). cgdapb functions as a tetramer, and each protomer is composed of two domains, an nterminal domain and a c-terminal domain. the n-terminal domain mainly ...201626502738
corrigendum: cell division in corynebacterineae.[this corrects the article on p. 132 in vol. 5, pmid: 24782835.].201627990143
structure-function profile of mmpl3, the essential mycolic acid transporter from mycobacterium tuberculosis.the mmpl family of proteins translocates complex (glyco)lipids and siderophores across the cell envelope of mycobacteria and closely related corynebacteriaceae and plays important roles in the biogenesis of the outer membrane of these organisms. despite their significance in the physiology and virulence of mycobacterium tuberculosis, and from the perspective of developing novel antituberculosis agents, little is known about their structure and mechanism of translocation. in this study, the essen ...201627737557
assembling of the mycobacterium tuberculosis cell wall core.the unique cell wall of mycobacteria is essential to their viability and the target of many clinically used anti-tuberculosis drugs and inhibitors under development. despite intensive efforts to identify the ligase(s) responsible for the covalent attachment of the two major heteropolysaccharides of the mycobacterial cell wall, arabinogalactan (ag) and peptidoglycan (pg), the enzyme or enzymes responsible have remained elusive. we here report on the identification of the two enzymes of mycobacter ...201627417139
structure and function of amtr in mycobacterium smegmatis: implications for post-transcriptional regulation of urea metabolism through a small antisense rna.soil-dwelling bacteria of the phylum actinomycetes generally harbor either glnr or amtr as a global regulator of nitrogen metabolism. mycobacterium smegmatis harbors both of these canonical regulators; glnr regulates the expression of key genes involved in nitrogen metabolism, while the function and signal transduction pathway of amtr in m. smegmatis remains largely unknown. here, we report the structure and function of the m. smegmatis amtr and describe the role of amtr in the regulation of nit ...201627640309
structural basis for cytokinin production by log from corynebacterium glutamicum."lonely guy" (log) has been identified as a cytokinin-producing enzyme in plants and plant-interacting fungi. the gene product of cg2612 from the soil-dwelling bacterium corynebacterium glutamicum was annotated as an ldc. however, the facts that c. glutamicum lacks an ldc and cg2612 has high amino acid similarity with log proteins suggest that cg2612 is possibly an log protein. to investigate the function of cg2612, we determined its crystal structure at a resolution of 2.3 å. cg2612 functions a ...201627507425
production of orotic acid by a klura3δ mutant of kluyveromyces lactis.we demonstrated that a klura3δ, mutant of the yeast kluyveromyces lactis is able to produce and secrete into the growth medium considerable amounts of orotic acid. using yeast extract-peptone-glucose (ypd) based media we optimized production conditions in flask and bioreactor cultures. with cells grown in ypd 5% glucose medium, the best production in flask was obtained with a 1:12.5 ratio for flask: culture volume, 180 rpm, 28°c and 200 mm mops for ph stabilization at neutral values (initial cul ...201626707627
engineering of corynebacterium glutamicum to utilize methyl acetate, a potential feedstock derived by carbonylation of methanol with co.the possibilities to utilize one-carbon substrates (c1) like co, methane and methanol have been explored as a cheap alternative feedstock in the biotechnology. for the first time, methyl acetate (meoac), which can be formed from carbonylation of methanol with co, was demonstrated to be an alternative carbon source for the cell growth of corynebacterium glutamicum as a model microbial cell factory. to do so, a carboxyl esterase activity was necessary to hydrolyze meoac to methanol and acetate. al ...201626970052
co-expression of endoglucanase and β-glucosidase in corynebacterium glutamicum dm1729 towards direct lysine fermentation from cellulose.the aim of the present study is the development of a consolidated bioprocess for the production of lysine with recombinant corynebacterium glutamicum dm1729 strains expressing endoglucanase and β-glucosidase genes. here, the endoglucanase genes from xanthomonas campestris xcc3521 and xcc2387 and betaglucosidase gene from saccharophagus degradans sde1394 were cloned in c. glutamicum dm1729 and expressed either extracellularly or on cell surface. the highest β-glucosidase activity of 9±0.5u/od600 ...201627020126
production of para-aminobenzoate by genetically engineered corynebacterium glutamicum and non-biological formation of an n-glucosyl byproduct.para-aminobenzoate (paba), a valuable chemical raw material, can be synthesized by most microorganisms. this aromatic compound is currently manufactured from petroleum-derived materials by chemical synthesis. to produce paba from renewable resources, its production by fermentation was investigated. the evaluation of the sensitivity to paba toxicity revealed that corynebacterium glutamicum had better tolerance to paba than several other microorganisms. to produce paba from glucose, genetically en ...201627471069
corynebacterium glutamicum possesses β-n-acetylglucosaminidase.in gram-positive corynebacterium glutamicum and other members of the suborder corynebacterianeae, which includes mycobacteria, cell elongation and peptidoglycan biosynthesis is mainly due to polar growth. c. glutamicum lacks an uptake system for the peptidoglycan constituent n-acetylglucosamine (glcnac), but is able to catabolize glcnac-6-phosphate. due to its importance in white biotechnology and in order to ensure more sustainable processes based on non-food renewables and to reduce feedstock ...201627492186
l-lysine production independent of the oxidative pentose phosphate pathway by corynebacterium glutamicum with the streptococcus mutans gapn gene.we have recently developed a corynebacterium glutamicum strain that generates nadph via the glycolytic pathway by replacing endogenous nad-dependent glyceraldehyde 3-phosphate dehydrogenase (gapa) with a nonphosphorylating nadp-dependent glyceraldehyde 3-phosphate dehydrogenase (gapn) from streptococcus mutans. strain re2, a suppressor mutant spontaneously isolated for its improved growth on glucose from the engineered strain, was proven to be a high-potential host for l-lysine production (taken ...201627044449
breeding l-arginine-producing strains by a novel mutagenesis method: atmospheric and room temperature plasma (artp).a plasma jet, driven by an active helium atom supplied with an atmospheric and room temperature plasma (artp) biological breeding system, was used as a novel method to breed l-arginine high-yielding strains. a mutant with resistance to l-homoarginine and 8-azaguaine, arg 3-15 (l-ha(r), 8-ag(r), l-his(-)), was screened after several rounds of screening. the l-arginine production of these mutants was more than that of the original strain, increased by 43.79% for arg 3-15. moreover, n-acetyl-l-glut ...201626460578
development of a new platform for secretory production of recombinant proteins in corynebacterium glutamicum.corynebacterium glutamicum, which has been for long an industrial producer of various l-amino acids, nucleic acids, and vitamins, is now also regarded as a potential host for the secretory production of recombinant proteins. to harness its potential as an industrial platform for recombinant protein production, the development of an efficient secretion system is necessary. particularly, regarding protein production in large-scale bioreactors, it would be appropriate to develop a secretory express ...201626134574
corynebacterium crudilactis sp. nov., isolated from raw cow's milk.a gram-stain-positive, rod-shaped bacterium (strain jz16t) was isolated from raw cow's milk from the bulk tank of a dairy farm in germany. the 16s rrna gene sequence of the isolate showed a similarity of 98.3 % to the nearest related type strain corynebacterium glutamicum atcc 13032t, a similarity of 97.6 % to corynebacterium deserti gimn1.010t and a similarity of 97.4 % to corynebacterium callunae dsm 20147t. determination of chemotaxonomic characteristics revealed oleic acid (18 : 1 cis 9) as ...201627666312
application of granular activated carbon/mnfe₂o₄ composite immobilized on c. glutamicum mtcc 2745 to remove as(iii) and as(v): kinetic, mechanistic and thermodynamic studies.the main objective of the present study was to investigate the efficiency of corynebacterium glutamicum mtcc 2745 immobilized on granular activated carbon/mnfe2o4 (gac/mnfe2o4) composite to treat high concentration of arsenic bearing wastewater. non-linear regression analysis was done for determining the best-fit kinetic model on the basis of three correlation coefficients and three error functions and also for predicting the parameters involved in kinetic models. the results showed that fractal ...201626322840
reconstruction and analysis of a genome-scale metabolic network of corynebacterium glutamicum s9114.corynebacterium glutamicum s9114 is commonly used for industrial glutamate production. therefore, a comprehensive understanding of the physiological and metabolic characteristics of c. glutamicum is important for developing its potential for industrial production. a genome-scale metabolic model, ijm658, was reconstructed based on genome annotation and literature mining. the model consists of 658 genes, 984 metabolites and 1065 reactions. the model quantitatively predicted c. glutamicum growth on ...201626392034
cutting the gordian knot: identifiability of anaplerotic reactions in corynebacterium glutamicum by means of (13) c-metabolic flux analysis.corynebacterium glutamicum is the major workhorse for the microbial production of several amino and organic acids. as long as these derive from tricarboxylic acid cycle intermediates, the activity of anaplerotic reactions is pivotal for a high biosynthetic yield. to determine single anaplerotic activities (13) c-metabolic flux analysis ((13) c-mfa) has been extensively used for c. glutamicum, however with different network topologies, inconsistent or poorly determined anaplerotic reaction rates. ...201626375179
involvement of the osrr gene in the hydrogen peroxide-mediated stress response of corynebacterium glutamicum.a transcriptional profile of the h2o2-adapted corynebacterium glutamicum ha strain reveals a list of upregulated regulatory genes. among them, we selected orf ncgl2298, designated osrr and analyzed its role in h2o2 adaptation. the osrr-deleted (δosrr) mutant had defective growth in minimal medium, which was even more pronounced in an osrr deletion mutant of an ha strain. the δosrr strain displayed increased sensitivity to h2o2. in addition to h2o2 sensitivity, the δosrr strain was found to be te ...201626433092
regulons of global transcription factors in corynebacterium glutamicum.corynebacterium glutamicum, a high gc content gram-positive soil bacterium in actinobacteria, has been used for the industrial production of amino acids and engineered to produce various compounds, including polymer building blocks and biofuels. since its genome sequence was first published, its versatile metabolic pathways and their genetic components and regulatory mechanisms have been extensively studied. previous studies on transcriptional factors, including two-component systems and σ facto ...201626496920
monomeric corynebacterium glutamicum n-acetyl glutamate kinase maintains sensitivity to l-arginine but has a lower intrinsic catalytic activity.n-acetyl glutamate kinase (nagk) is a key enzyme in the synthesis of l-arginine, and l-arginine-sensitive nagk typically has hexameric architecture. defining the relationship between this architecture and l-arginine inhibition can provide a foundation to identify the key amino acids involved in the allosteric regulation network of l-arginine. in the present study, the key amino acids in the n-terminal helix (n-helix) of corynebacterium glutamicum (cg) nagk required for hexamer formation were det ...201626512006
effects of phosphoenolpyruvate carboxylase desensitization on glutamic acid production in corynebacterium glutamicum atcc 13032.phosphoenolpyruvate carboxylase (pepc) in corynebacterium glutamicum atcc13032, a glutamic-acid producing actinobacterium, is subject to feedback inhibition by metabolic intermediates such as aspartic acid and 2-oxoglutaric acid, which implies the importance of pepc in replenishing oxaloacetic acid into the tca cycle. here, we investigated the effects of feedback-insensitive pepc on glutamic acid production. a single amino-acid substitution in pepc, d299n, was found to relieve the feedback contr ...201626168906
metabolic engineering of a laboratory-evolved thermobifida fusca muc strain for malic acid production on cellulose and minimal treated lignocellulosic biomass.malic acid is mainly used as an acidulant and taste enhancer in the beverage and food industry. previously, a mutant strain thermobifida fusca muc, obtained by adaptive evolution was found to accumulate malic acid on cellulose with low yield. in this study, the malic acid synthesis pathway in t. fusca muc was confirmed to be from phosphoenolpyruvate to oxaloacetate, followed by reduction of oxaloacetate to malate. to increase the yield of malic acid by the muc strain significantly, the carbon fl ...201726439318
rational design of substrate binding pockets in polyphosphate kinase for use in cost-effective atp-dependent cascade reactions.adenosine-5'-triphosphate (atp) is the energy equivalent of the living system. polyphosphate (polyp) is the ancient energy storage equivalent of organisms. polyphosphate kinases (ppks) catalyze the polyp formation or atp formation, to store energy or to regenerate atp, respectively. however, most ppks are active only in the presence of long polyps, which are more difficult and more expensive to generate than the short polyps. we investigated the ppk preference towards polyps by site-directed mut ...201728417169
crispr-cpf1 assisted genome editing of corynebacterium glutamicum.corynebacterium glutamicum is an important industrial metabolite producer that is difficult to genetically engineer. although the streptococcus pyogenes (sp) crispr-cas9 system has been adapted for genome editing of multiple bacteria, it cannot be introduced into c. glutamicum. here we report a francisella novicida (fn) crispr-cpf1-based genome-editing method for c. glutamicum. crispr-cpf1, combined with single-stranded dna (ssdna) recombineering, precisely introduces small changes into the bact ...201728469274
the antibacterial prodrug activator rv2466c is a mycothiol-dependent reductase in the oxidative stress response of mycobacterium tuberculosis.the mycobacterium tuberculosis rv2466c gene encodes an oxidoreductase enzyme annotated as dsba. it has a cpwc active-site motif embedded within its thioredoxin fold domain and mediates the activation of the prodrug tp053, a thienopyrimidine derivative that kills both replicating and nonreplicating bacilli. however, its mode of action and actual enzymatic function in m. tuberculosis have remained enigmatic. in this study, we report that rv2466c is essential for bacterial survival under h2o2 stres ...201728620052
production of 5-aminovaleric acid in recombinant corynebacterium glutamicum strains from a miscanthus hydrolysate solution prepared by a newly developed miscanthus hydrolysis process.this study examined nine expired industrial corynebacterium glutamicum strains with high lysine producing capability for enhanced production of 5-ava. c. glutamicum kctc 1857 exhibiting the highest lysine production was transformed with either original pseudomonas putida davba genes, encoding the 5-ava biosynthesis pathway, or c. glutamicum codon-optimized davba genes. c. glutamicum kctc 1857 expressing the original genes had superior cell viability and 5-ava production capability compared to th ...201728579174
bioprocess engineering to produce 9-(nonanoyloxy) nonanoic acid by a recombinant corynebacterium glutamicum-based biocatalyst.here, corynebacterium glutamicum atcc13032 expressing baeyer-villiger monooxygenase from pseudomonas putida kt2440 was designed to produce 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid. diverse parameters including cultivation and reaction temperatures, type of detergent, and ph were found to improve biotransformation efficiency. the optimal temperature of cultivation for the production of 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid using whole cells of recombinant c. glutami ...201728567672
bio-based production of dimethyl itaconate from rice wine waste-derived itaconic acid.dimethyl itaconate is an important raw material for copolymerization, but it is not synthesized from itaconic acid by organisms. moreover, corynebacterium glutamicum is used as an important industrial host for the production of organic acids, but it does not metabolize itaconic acid. therefore, the biosynthetic route towards dimethyl itaconate from itaconic acid is highly needed. in this study, we developed a biological procedure for dimethyl itaconate production from rice wine waste-derived ita ...201728846199
construction of recombinant pdu metabolosome shells for small molecule production in corynebacterium glutamicum.bacterial microcompartments have significant potential in the area of industrial biotechnology for the production of small molecules, especially involving metabolic pathways with toxic or volatile intermediates. corynebacterium glutamicum is an established industrial workhorse for the production of amino acids and has been investigated for the production of a variety of further value-added products. herein, we describe components for the establishment of bacterial microcompartments as production ...201728826205
escherichia coli yjjpb genes encode a succinate transporter important for succinate production.under anaerobic conditions, escherichia coli produces succinate from glucose via the reductive tricarboxylic acid cycle. to date, however, no genes encoding succinate exporters have been established in e. coli. therefore, we attempted to identify genes encoding succinate exporters by screening an e. coli mg1655 genome library. we identified the yjjpb genes as candidates encoding a succinate transporter, which enhanced succinate production in pantoea ananatis under aerobic conditions. a complemen ...201728673128
current status on metabolic engineering for the production of l-aspartate family amino acids and derivatives.the l-aspartate amino acids (afaas) are constituted of l-aspartate, l-lysine, l-methionine, l-threonine and l-isoleucine. except for l-aspartate, afaas are essential amino acids that cannot be synthesized by humans and most farm animals, and thus possess wide applications in food, animal feed, pharmaceutical and cosmetics industries. to date, a number of amino acids, including afaas have been industrially produced by microbial fermentation. however, the overall metabolic and regulatory mechanism ...201728579173
production of amino acids - genetic and metabolic engineering approaches.the biotechnological production of amino acids occurs at the million-ton scale and annually about 6milliontons of l-glutamate and l-lysine are produced by escherichia coli and corynebacterium glutamicum strains. l-glutamate and l-lysine production from starch hydrolysates and molasses is very efficient and access to alternative carbon sources and new products has been enabled by metabolic engineering. this review focusses on genetic and metabolic engineering of amino acid producing strains. in p ...201728552565
a new metabolic route for the fermentative production of 5-aminovalerate from glucose and alternative carbon sources.here, a new metabolic pathway for the production of 5-aminovalerate (5ava) from l-lysine via cadaverine as intermediate was established and this three-step-pathway comprises l-lysine decarboxylase (ldcc), putrescine transaminase (pata) and γ-aminobutyraldehyde dehydrogenase (patd). since corynebacterium glutamicum is used for industrial l-lysine production, the pathway was established in this bacterium. upon expression of ldcc, pata and patd from escherichia coli in c. glutamicum wild type, prod ...201728522202
comparative analysis of the expression level of recombinant ginsenoside-transforming β-glucosidase in gras hosts and mass production of the ginsenoside rh2-mix.the ginsenoside rh2, a pharmaceutically active component of ginseng, is known to have anticancer and antitumor effects. however, white ginseng and red ginseng have extremely low concentrations of rh2 or rh2-mix [20(s)-rh2, 20(r)-rh2, rk2, and rh3]. to enhance the production of food-grade ginsenoside rh2, an edible enzymatic bioconversion technique was developed adopting gras host strains. a β-glucosidase (bglpm), which has ginsenoside conversion ability, was expressed in three gras host strains ...201728423055
functional analysis of arabinofuranosidases and a xylanase of corynebacterium alkanolyticum for arabinoxylan utilization in corynebacterium glutamicum.xylooligosaccharides (xoss) and arabinoxylooligosaccharides (axoss) are major oligosaccharides derived from arabinoxylan. in our previous report, corynebacterium glutamicum was engineered to utilize xoss by introducing corynebacterium alkanolyticum xyloside transporter and β-xylosidase. however, this strain was unable to consume axoss due to the absence of α-l-arabinofuranosidase activity. in this study, to confer axos utilization ability on c. glutamicum, two putative arabinofuranosidase genes ...201728409383
mutagenesis and redox partners analysis of the p450 fatty acid decarboxylase oletje.the cytochrome p450 enzyme oletje from jeotgalicoccus sp. atcc 8456 is capable of converting free long-chain fatty acids into α-alkenes via one-step oxidative decarboxylation in presence of h2o2 as cofactor or using redox partner systems. this enzyme has attracted much attention due to its intriguing but unclear catalytic mechanism and potential application in biofuel production. here, we investigated the functionality of a select group of residues (arg245, cys365, his85, and ile170) in the acti ...201728276499
sequence-based identification of inositol monophosphatase-like histidinol-phosphate phosphatases (hisn) in corynebacterium glutamicum, actinobacteria, and beyond.the eighth step of l-histidine biosynthesis is carried out by an enzyme called histidinol-phosphate phosphatase (holpase). three unrelated holpase families are known so far. two of them are well studied: had-type holpases known from gammaproteobacteria like escherichia coli or salmonella enterica and php-type holpases known from yeast and firmicutes like bacillus subtilis. however, the third family of holpases, the inositol monophosphatase (impase)-like holpases, present in actinobacteria like c ...201728720084
a truncated sph12-38 with potent antimicrobial activity showing resistance against bacterial challenge in oryzias melastigma.antimicrobial peptides (amps) represent an efficient part of innate immunity and are found in a variety of life. among them histone 2a (h2a), as a promising class of amps, attracts great attention, but the in vivo mechanism of h2a derived amp is still less known. based on the acquisition of sphistin, a synthetic 38-amino acid h2a derived peptide from scylla paramamosain, as reported in our previous study, was truncated into three short fragments (sph12-38, sph20-38 and sph30-38) and further inve ...201728600196
1,5-diaminopentane production from xylooligosaccharides using metabolically engineered corynebacterium glutamicum displaying beta-xylosidase on the cell surface.xylooligosaccharide-assimilating corynebacterium glutamicum strains were constructed using metabolic engineering and cell surface display techniques. first, c. glutamicum was metabolically engineered to create lysine-producing strains. beta-xylosidase bsu17580 derived from bacillus subtilis was then expressed on the c. glutamicum cell surface using porh anchor protein, and enzymes involved in the xylose assimilation pathway were also expressed. metabolic engineering had no effect on the activity ...201728599919
molecular engineering of l-aspartate-α-decarboxylase for improved activity and catalytic stability.β-alanine is an important precursor for the production of food additives, pharmaceuticals, and nitrogen-containing chemicals. compared with the conventional chemical routes for β-alanine production, the biocatalytic routes using l-aspartate-α-decarboxylase (adc) are more attractive when energy and environment are concerned. however, adc's poorly understood properties and its inherent mechanism-based inactivation significantly limited the application of this enzyme. in this study, three genes enc ...201728589224
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