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application of granular activated carbon/mnfe₂o₄ composite immobilized on c. glutamicum mtcc 2745 to remove as(iii) and as(v): kinetic, mechanistic and thermodynamic studies.the main objective of the present study was to investigate the efficiency of corynebacterium glutamicum mtcc 2745 immobilized on granular activated carbon/mnfe2o4 (gac/mnfe2o4) composite to treat high concentration of arsenic bearing wastewater. non-linear regression analysis was done for determining the best-fit kinetic model on the basis of three correlation coefficients and three error functions and also for predicting the parameters involved in kinetic models. the results showed that fractal ...201626322840
the pupylation machinery is involved in iron homeostasis by targeting the iron storage protein ferritin.the balance of sufficient iron supply and avoidance of iron toxicity by iron homeostasis is a prerequisite for cellular metabolism and growth. here we provide evidence that, in actinobacteria, pupylation plays a crucial role in this process. pupylation is a posttranslational modification in which the prokaryotic ubiquitin-like protein pup is covalently attached to a lysine residue in target proteins, thus resembling ubiquitination in eukaryotes. pupylated proteins are recognized and unfolded by ...201627078093
enhanced succinic acid production in corynebacterium glutamicum with increasing the available nadh supply and glucose consumption rate by decreasing h(+)-atpase activity.to enhance succinic acid production in corynebacterium glutamicum by increasing the supply of nadh and the rate of glucose consumption by decreasing h(+)-atpase activity.201627053082
corynebacterium glutamicum for sustainable bioproduction: from metabolic physiology to systems metabolic engineering.since its discovery 60 years ago, corynebacterium glutamicum has evolved into a workhorse for industrial biotechnology. traditionally well known for its remarkable capacity to produce amino acids, this gram-positive soil bacterium, has become a flexible, efficient production platform for various bulk and fine chemicals, materials, and biofuels. the central turnstile of all these achievements is our excellent understanding of its metabolism and physiology. this knowledge base, together with innov ...201627872959
application of crispri in corynebacterium glutamicum for shikimic acid production.to construct, test and exploit the crispri system for enhancement of shikimic acid production with corynebacterium glutamicum.201627623797
construction of genetic parts from the corynebacterium glutamicum genome with high expression activities.to construct effective genetic expression parts controlling transcription and translation initiation for synthetic biology and heterologous expression in corynebacterium glutamicum.201627580890
global transcriptomic analysis of the response of corynebacterium glutamicum to vanillin.lignocellulosic biomass is an abundant and renewable resource for biofuels and bio-based chemicals. vanillin is one of the major phenolic inhibitors in biomass production using lignocellulose. to assess the response of corynebacterium glutamicum to vanillin stress, we performed a global transcriptional response analysis. the transcriptional data showed that the vanillin stress not only affected the genes involved in degradation of vanillin, but also differentially regulated several genes related ...201627760214
glutamine metabolism of corynebacterium glutamicum: role of the glutaminase glsk.corynebacterium glutamicum is able to metabolize different nitrogen and carbon sources. in standard minimal media, ammonium and urea typically serve as nitrogen source and glucose or sucrose as carbon and energy source; however, amino acids might also play a role as nitrogen, carbon and energy source. in this study, the function of the putative glutaminase glsk was investigated. a glsk deletion strain showed impaired growth with l-glutamine as carbon and energy source, while growth was improved ...201627754855
light-controlled cell factories: employing photocaged isopropyl-β-d-thiogalactopyranoside for light-mediated optimization of lac promoter-based gene expression and (+)-valencene biosynthesis in corynebacterium glutamicum.precise control of microbial gene expression resulting in a defined, fast, and homogeneous response is of utmost importance for synthetic bio(techno)logical applications. however, even broadly applied biotechnological workhorses, such as corynebacterium glutamicum, for which induction of recombinant gene expression commonly relies on the addition of appropriate inducer molecules, perform moderately in this respect. light offers an alternative to accurately control gene expression, as it allows f ...201627520809
roles of n287 in catalysis and product formation of amylomaltase from corynebacterium glutamicum.amylomaltase catalyzes intermolecular and intramolecular transglucosylation reactions to form linear and cyclic oligosaccharides, respectively. the aim of this work is to investigate the structure-function relationship of amylomaltase from a mesophilic corynebacterium glutamicum (cgam). site-directed mutagenesis was performed to substitute tyr for asn287 (n287y) to determine its role in controlling amylomaltase activity and product formation. expression of the wild-type (wt) and n287y was achiev ...201627507216
overexpression of mycothiol disulfide reductase enhances corynebacterium glutamicum robustness by modulating cellular redox homeostasis and antioxidant proteins under oxidative stress.mycothiol (msh) is the dominant low-molecular-weight thiol (lmwt) unique to high-(g+c)-content gram-positive actinobacteria, such as corynebacterium glutamicum, and is oxidised into its disulfide form mycothiol disulfide (mssm) under oxidative conditions. mycothiol disulfide reductase (mtr), an nadph-dependent enzyme, reduces mssm to msh, thus maintaining intracellular redox homeostasis. in this study, a recombinant plasmid was constructed to overexpress mtr in c. glutamicum using the expression ...201627383057
metabolic engineering of corynebacterium glutamicum for methionine production by removing feedback inhibition and increasing nadph level.relieving the feedback inhibition of key enzymes in a metabolic pathway is frequently the first step of producer-strain construction by genetic engineering. however, the strict feedback regulation exercised by microorganisms in methionine biosynthesis often makes it difficult to produce methionine at a high level. in this study, corynebacterium glutamicum atcc 13032 was metabolically engineered for methionine production. first, the metd gene encoding the methionine uptake system was deleted to a ...201627255137
a method for simultaneous gene overexpression and inactivation in the corynebacterium glutamicum genome.the gene integration method is an important tool to stably express desirable genes in bacteria. to avoid heavy workload and cost, we constructed a rapid and efficient method for genome modification. this method depended on a mobilizable plasmid, which contains a p tac promoter, an introduced multiple cloning site (imcs), and rrnbt1t2 terminator. briefly, the mobilizable plasmid pk18-mbpmt with the p tac-imcs-rrnbt1t2 cartridge derived from pk18mobsacb was prepared to directly integrate hetero-/h ...201627377799
biotypes analysis of corynebacterium glutamicum growing in dicarboxylic acids demonstrates the existence of industrially-relevant intra-species variations.production enhancement of industrial microbial products or strains has been traditionally tackled by mutagenesis with chemical methods, irradiation or genetic manipulation. however, the final yield increase must go hand in hand with the resistance increasing against the usual inherent toxicity of the final products. few studies have been carried out on resistance improvement and even fewer on the initial selection of naturally-generated biotypes, which could decrease the artificial mutagenesis. ...201627371347
crystal structure of amylomaltase from corynebacterium glutamicum.amylomaltase is an essential enzyme in maltose utilization and maltodextrin metabolism, and it has been industrially used for the production of cyclodextrin and modification of starch. we determined the crystal structure of amylomaltase from corynebacterium glutamicum (cgam) at a resolution of 1.7 å. although cgam forms a dimer without nacl, it exists as a monomer in physiological concentration of nacl. cgam is composed of n- and c-terminal domains, which can be further divided into two and four ...201627366969
the small 6c rna of corynebacterium glutamicum is involved in the sos response.the 6c rna family is a class of small rnas highly conserved in actinobacteria, including the genera mycobacterium, streptomyces and corynebacterium whose physiological function has not yet been elucidated. we found that strong transcription of the cgb_03605 gene, which encodes 6c rna in c. glutamicum, was driven by the siga- and sigb-dependent promoter pcgb_03605. 6c rna was detected at high level during exponential growth phase (180 to 240 molcules per cell) which even increased at the entry of ...201627362471
a novel acee mutation leading to a better growth profile and a higher l-serine production in a high-yield l-serine-producing corynebacterium glutamicum strain.a comparative genomic analysis was performed to study the genetic variations between the l-serine-producing strain corynebacterium glutamicum syps-062 and the mutant strain syps-062-33a, which was derived from syps-062 by random mutagenesis with enhanced l-serine production. some variant genes between the two strains were reversely mutated or deleted in the genome of syps-062-33a to verify the influences of the gene mutations introduced by random mutagenesis. it was found that a his-594 → tyr mu ...201627344574
systems pathway engineering of corynebacterium crenatum for improved l-arginine production.l-arginine is an important amino acid in food and pharmaceutical industries. until now, the main production method of l-arginine in china is the highly polluting keratin acid hydrolysis. the industrial level l-arginine production by microbial fermentation has become an important task. in previous work, we obtained a new l-arginine producing corynebacterium crenatum (subspecies of corynebacterium glutamicum) through screening and mutation breeding. in this work, we performed systems pathway engin ...201627338253
transcriptional regulation of the β-type carbonic anhydrase gene bca by rama in corynebacterium glutamicum.carbonic anhydrase catalyzes the reversible hydration of carbon dioxide to bicarbonate and maintains the balance of co2/hco3- in the intracellular environment, specifically for carboxylation/decarboxylation reactions. in corynebacterium glutamicum, two putative genes, namely the bca (cg2954) and gca (cg0155) genes, coding for β-type and γ-type carbonic anhydrase, respectively, have been identified. we here analyze the transcriptional organization of these genes. the transcriptional start site (t ...201627119954
formation of xylitol and xylitol-5-phosphate and its impact on growth of d-xylose-utilizing corynebacterium glutamicum strains.wild-type corynebacterium glutamicum has no endogenous metabolic activity for utilizing the lignocellulosic pentose d-xylose for cell growth. therefore, two different engineering approaches have been pursued resulting in platform strains harbouring a functional version of either the isomerase (iso) or the weimberg (wmb) pathway for d-xylose assimilation. in a previous study we found for c. glutamicum wmb by-product formation of xylitol during growth on d-xylose and speculated that the observed l ...201627297548
corynebacterium glutamicum mtcc 2745 immobilized on granular activated carbon/mnfe2o4 composite: a novel biosorbent for removal of as(iii) and as(v) ions.the optimization of biosorption/bioaccumulation process of both as(iii) and as(v) has been investigated by using the biosorbent; biofilm of corynebacterium glutamicum mtcc 2745 supported on granular activated carbon/mnfe2o4 composite (mgac). the presence of functional groups on the cell wall surface of the biomass that may interact with the metal ions was proved by ft-ir. to determine the most appropriate correlation for the equilibrium curves employing the procedure of the non-linear regression ...201627289352
production of 2-methyl-1-butanol and 3-methyl-1-butanol in engineered corynebacterium glutamicum.the pentanol isomers 2-methyl-1-butanol and 3-methyl-1-butanol represent commercially interesting alcohols due to their potential application as biofuels. for a sustainable microbial production of these compounds, corynebacterium glutamicum was engineered for producing 2-methyl-1-butanol and 3-methyl-1-butanol via the ehrlich pathway from 2-keto-3-methylvalerate and 2-ketoisocaproate, respectively. in addition to an already available 2-ketoisocaproate producer, a 2-keto-3-methylvalerate accumula ...201627746323
transcriptome and multivariable data analysis of corynebacterium glutamicum under different dissolved oxygen conditions in bioreactors.dissolved oxygen (do) is an important factor in the fermentation process of corynebacterium glutamicum, which is a widely used aerobic microbe in bio-industry. herein, we described rna-seq for c. glutamicum under different do levels (50%, 30% and 0%) in 5 l bioreactors. multivariate data analysis (mvda) models were used to analyze the rna-seq and metabolism data to investigate the global effect of do on the transcriptional distinction of the substance and energy metabolism of c. glutamicum. the ...201627907077
a toolbox of genetically encoded fret-based biosensors for rapid l-lysine analysis.background: the fast development of microbial production strains for basic and fine chemicals is increasingly carried out in small scale cultivation systems to allow for higher throughput. such parallelized systems create a need for new rapid online detection systems to quantify the respective target compound. in this regard, biosensors, especially genetically encoded förster resonance energy transfer (fret)-based biosensors, offer tremendous opportunities. as a proof-of-concept, we have created ...201627690044
transcriptomic analysis for elucidating the physiological effects of 5-aminolevulinic acid accumulation on corynebacterium glutamicum.5-aminolevulinic acid (ala), the committed intermediate of the heme biosynthetic pathway, attracts close attention among researchers because of its potential applications to cancer treatment and agriculture. overexpression of heterologous hema and heml, which encode glutamyl-trna reductase and glutamate-1-semialdehyde aminotransferase, respectively, in corynebacterium glutamicum produces ala, although whether ala accumulation causes unintended effects on the host is unknown. here we used an inte ...201627664748
elucidation of the regulatory role of the fructose operon reveals a novel target for enhancing the nadph supply in corynebacterium glutamicum.the performance of corynebacterium glutamicum cell factories producing compounds which rely heavily on nadph has been reported to depend on the sugar being metabolized. while some aspects of this phenomenon have been elucidated, there are still many unresolved questions as to how sugar metabolism is linked to redox and to the general metabolism. we here provide new insights into the regulation of the metabolism of this important platform organism by systematically characterizing mutants carrying ...201627553884
transcription of sialic acid catabolism genes in corynebacterium glutamicum is subject to catabolite repression and control by the transcriptional repressor nanr.corynebacterium glutamicum metabolizes sialic acid (neu5ac) to fructose-6-phosphate (fructose-6p) via the consecutive activity of the sialic acid importer siaefgi, n-acetylneuraminic acid lyase (nana), n-acetylmannosamine kinase (nank), n-acetylmannosamine-6p epimerase (nane), n-acetylglucosamine-6p deacetylase (naga), and glucosamine-6p deaminase (nagb). within the cluster of the three operons nagab, nanake, and siaefgi for neu5ac utilization a fourth operon is present, which comprises cg2936, ...201627274030
use of in vitro transcription system for analysis of corynebacterium glutamicum promoters recognized by two sigma factors.promoter activities in corynebacterium glutamicum strains with deletions of genes encoding sigma factors of rna polymerase suggested that transcription from some promoters is controlled by two sigma factors. to prove that different sigma factors are involved in the recognition of selected corynebacterium glutamicum promoters, in vitro transcription system was applied. it was found that a typical housekeeping promoter pper interacts with the alternative sigma factor σ(b) in addition to the primar ...201627270733
silencing of cryptic prophages in corynebacterium glutamicum.dna of viral origin represents a ubiquitous element of bacterial genomes. its integration into host regulatory circuits is a pivotal driver of microbial evolution but requires the stringent regulation of phage gene activity. in this study, we describe the nucleoid-associated protein cgps, which represents an essential protein functioning as a xenogeneic silencer in the gram-positive corynebacterium glutamicum cgps is encoded by the cryptic prophage cgp3 of the c. glutamicum strain atcc 13032 and ...201627492287
improvement of the intracellular environment for enhancing l-arginine production of corynebacterium glutamicum by inactivation of h2o2-forming flavin reductases and optimization of atp supply.l-arginine, a semi essential amino acid, is an important amino acid in food flavoring and pharmaceutical industries. its production by microbial fermentation is gaining more and more attention. in previous work, we obtained a new l-arginine producing corynebacterium crenatum (subspecies of corynebacterium glutamicum) through mutation breeding. in this work, we enhanced l-arginine production through improvement of the intracellular environment. first, two nad(p)h-dependent h2o2-forming flavin red ...201627474351
the obligate respiratory supercomplex from actinobacteria.actinobacteria are closely linked to human life as industrial producers of bioactive molecules and as human pathogens. respiratory cytochrome bcc complex and cytochrome aa3 oxidase are key components of their aerobic energy metabolism. they form a supercomplex in the actinobacterial species corynebacterium glutamicum. with comprehensive bioinformatics and phylogenetic analysis we show that genes for cyt bcc-aa3 supercomplex are characteristic for actinobacteria (actinobacteria and acidimicrobiia ...201627472998
erratum to: the actinobacterium corynebacterium glutamicum, an industrial workhorse.this erratum is being published to correct the 3rd author's name of above manuscript by lee et al. that was published in journal of microbiology and biotechnology (2016, 26: 807-822). the 3rd author name(eungsoo kim) should appear as 'eung-soo kim'.201627452344
mycothiol protects corynebacterium glutamicum against acid stress via maintaining intracellular ph homeostasis, scavenging ros, and s-mycothiolating mete.mycothiol (msh) plays a major role in protecting cells against oxidative stress and detoxification from a broad range of exogenous toxic agents. in the present study, we reveal that intracellular msh contributes significantly to the adaptation to acidic conditions in the model organism corynebacterium glutamicum. we present evidence that msh confers c. glutamicum with the ability to adapt to acidic conditions by maintaining phi homeostasis, scavenging reactive oxygen species (ros), and protectin ...201627250661
structural characterization of heme environmental mutants of cghmut that shuttles heme molecules to heme transporters.corynebacteria contain a heme uptake system encoded in hmutuv genes, in which hmut protein acts as a heme binding protein to transport heme to the cognate transporter hmuuv. the crystal structure of hmut from corynebacterium glutamicum (cghmut) reveals that heme is accommodated in the central cleft with his141 and tyr240 as the axial ligands and that tyr240 forms a hydrogen bond with arg242. in this work, the crystal structures of h141a, y240a, and r242a mutants were determined to understand the ...201627240352
cathodes enhance corynebacterium glutamicum growth with nitrate and promote acetate and formate production.the industrially important corynebacterium glutamicum can only incompletely reduce nitrate into nitrite which then accumulates and inhibits growth. herein we report that cathodes can resolve this problem and enhance glucose fermentation and growth by promoting nitrite reduction. cell growth was inhibited at relatively high potentials but was significant when potentials were more reductive (-1.20v with anthraquinone-2-sulfonate as redox mediator or -1.25v vs. ag/agcl). under these conditions, glu ...201627235972
metabolic engineering of corynebacterium glutamicum atcc13032 to produce s-adenosyl-l-methionine.as an important biological methyl group donor, s-adenosyl-l-methionine is used as nutritional supplement or drug for various diseases, but bacterial strains that can efficiently produce s-adenosyl-l-methionine are not available. in this study, corynebacterium glutamicum strain hw104 which can accumulate s-adenosyl-l-methionine was constructed from c. glutamicum atcc13032 by deleting four genes thrb, metb, mcbr and ncgl2640, and six genes metk, vgb, lysc(m), hom(m), metx and mety were overexpress ...201626777246
structure of amtr, the global nitrogen regulator of corynebacterium glutamicum, in free and dna-bound forms.corynebacterium glutamicum is a bacterium used for industrial amino acid production, and understanding its metabolic pathway regulation is of high biotechnological interest. here, we report crystal structures of amtr, the global nitrogen regulator of c. glutamicum, in apo (2.25-å and 2.65-å resolution) and dna-bound (3-å resolution) forms. these structures reveal an all-α homodimeric tetr family regulator composed of a helix-turn-helix-hosting n-terminal dna-binding domain and a c-terminal dimer ...201626744254
a novel pyruvate kinase and its application in lactic acid production under oxygen deprivation in corynebacterium glutamicum.pyruvate kinase (pyk) catalyzes the generation of pyruvate and atp in glycolysis and functions as a key switch in the regulation of carbon flux distribution. both the substrates and products of pyk are involved in the tricarboxylic acid cycle, anaplerosis and energy anabolism, which places pyk at a primary metabolic intersection. pyks are highly conserved in most bacteria and lower eukaryotes. corynebacterium glutamicum is an industrial workhorse for the production of various amino acids and org ...201627852252
pathway construction in corynebacterium glutamicum and strain engineering to produce rare sugars from glycerol.rare sugars are valuable natural products widely used in pharmaceutical and food industries. in this study, we expected to synthesize rare ketoses from abundant glycerol using dihydroxyacetone phosphate (dhap)-dependent aldolases. first, a new glycerol assimilation pathway was constructed to synthesize dhap. the enzymes which convert glycerol to 3-hydroxypropionaldehyde and l-glyceraldehyde were selected, and their corresponding aldehyde synthesis pathways were constructed in vivo. four aldol pa ...201627998065
metabolic engineering of cyanobacteria for the photosynthetic production of succinate.succinate is an important commodity chemical currently used in the food, pharmaceutical, and polymer industries. it can also be chemically converted into other major industrial chemicals such as 1,4-butanediol, butadiene, and tetrahydrofuran. here we metabolically engineered a model cyanobacterium synechococcus elongatus pcc 7942 to photosynthetically produce succinate. we expressed the genes encoding for α-ketoglutarate decarboxylase and succinate semialdehyde dehydrogenase in s. elongatus pcc ...201627989804
metabolic engineering of corynebacterium glutamicum for shikimate overproduction by growth-arrested cell reaction.corynebacterium glutamicum with the ability to simultaneously utilize glucose/pentose mixed sugars was metabolically engineered to overproduce shikimate, a valuable hydroaromatic compound used as a starting material for the synthesis of the anti-influenza drug oseltamivir. to achieve this, the shikimate kinase and other potential metabolic activities for the consumption of shikimate and its precursor dehydroshikimate were inactivated. carbon flux toward shikimate synthesis was enhanced by overex ...201627553883
construction of a corynebacterium glutamicum platform strain for the production of stilbenes and (2s)-flavanones.corynebacterium glutamicum is an important organism in industrial biotechnology for the microbial production of bulk chemicals, in particular amino acids. however, until now activity of a complex catabolic network for the degradation of aromatic compounds averted application of c. glutamicum as production host for aromatic compounds of pharmaceutical or biotechnological interest. in the course of the construction of a suitable c. glutamicum platform strain for plant polyphenol production, four g ...201627288926
involvement of the osrr gene in the hydrogen peroxide-mediated stress response of corynebacterium glutamicum.a transcriptional profile of the h2o2-adapted corynebacterium glutamicum ha strain reveals a list of upregulated regulatory genes. among them, we selected orf ncgl2298, designated osrr and analyzed its role in h2o2 adaptation. the osrr-deleted (δosrr) mutant had defective growth in minimal medium, which was even more pronounced in an osrr deletion mutant of an ha strain. the δosrr strain displayed increased sensitivity to h2o2. in addition to h2o2 sensitivity, the δosrr strain was found to be te ...201626433092
mutagenesis for improvement of activity and thermostability of amylomaltase from corynebacterium glutamicum.this work aims to improve thermostability of amylomaltase from a mesophilic corynebacterium glutamicum (cgam) by random and site-directed mutagenesis. from error prone pcr, a mutated cgam with higher thermostability at 50 °c compared to the wild-type was selected and sequenced. the result showed that the mutant contains a single mutation of a406v. site-directed mutagenesis was then performed to construct a406v and a406l. both mutated cgams showed higher intermolecular transglucosylation activity ...201626875536
optimization of large-ring cyclodextrin production from starch by amylomaltase from corynebacterium glutamicum and effect of organic solvent on product size.to increase yield of starch conversion to large-ring cyclodextrins (lr-cds) by amylomaltase from corynebacterium glutamicum (cgam).201626849173
the actinobacterium corynebacterium glutamicum, an industrial workhorse.starting as a glutamate producer, corynebacterium glutamicum has played a variety of roles in the industrial production of amino acids, one of the most important areas of white biotechnology. from shortly after its genome information became available, c. glutamicum has been applied in various production processes for value-added chemicals, fuels, and polymers, as a key organism in industrial biotechnology alongside the surprising progress in systems biology and metabolic engineering. in addition ...201626838341
corynebacterium glutamicum metabolic engineering with crispr interference (crispri).corynebacterium glutamicum is an important organism for the industrial production of amino acids. metabolic pathways in this organism are usually engineered by conventional methods such as homologous recombination, which depends on rare double-crossover events. to facilitate the mapping of gene expression levels to metabolic outputs, we applied crispr interference (crispri) technology using deactivated cas9 (dcas9) to repress genes in c. glutamicum. we then determined the effects of target repre ...201626829286
characterization of a unique pathway for 4-cresol catabolism initiated by phosphorylation in corynebacterium glutamicum.4-cresol is not only a significant synthetic intermediate for production of many aromatic chemicals, but also a priority environmental pollutant because of its toxicity to higher organisms. in our previous studies, a gene cluster implicated to be involved in 4-cresol catabolism, crecdefghir, was identified in corynebacterium glutamicum and partially characterized in vivo. in this work, we report on the discovery of a novel 4-cresol biodegradation pathway that employs phosphorylated intermediates ...201626817843
modular optimization of a hemicellulose-utilizing pathway in corynebacterium glutamicum for consolidated bioprocessing of hemicellulosic biomass.hemicellulose, which is the second most abundant polysaccharide in nature after cellulose, has the potential to become a major feedstock for microbial fermentation to produce various biofuels and chemicals. to utilize hemicellulose economically, it is necessary to develop a consolidated bioprocess (cbp), in which all processes from biomass degradation to the production of target products occur in a single bioreactor. here, we report a modularly engineered corynebacterium glutamicum strain suitab ...201626808593
adaptive evolution and metabolic engineering of a cellobiose- and xylose- negative corynebacterium glutamicum that co-utilizes cellobiose and xylose.an efficient microbial cell factory requires a microorganism that can utilize a broad range of substrates to economically produce value-added chemicals and fuels. the industrially important bacterium corynebacterium glutamicum has been studied to broaden substrate utilizations for lignocellulose-derived sugars. however, c. glutamicum atcc 13032 is incapable of pts-dependent utilization of cellobiose because it has missing genes annotated to β-glucosidases (bg) and cellobiose-specific pts permeas ...201626801253
reconstruction and analysis of a genome-scale metabolic network of corynebacterium glutamicum s9114.corynebacterium glutamicum s9114 is commonly used for industrial glutamate production. therefore, a comprehensive understanding of the physiological and metabolic characteristics of c. glutamicum is important for developing its potential for industrial production. a genome-scale metabolic model, ijm658, was reconstructed based on genome annotation and literature mining. the model consists of 658 genes, 984 metabolites and 1065 reactions. the model quantitatively predicted c. glutamicum growth on ...201626392034
cutting the gordian knot: identifiability of anaplerotic reactions in corynebacterium glutamicum by means of (13) c-metabolic flux analysis.corynebacterium glutamicum is the major workhorse for the microbial production of several amino and organic acids. as long as these derive from tricarboxylic acid cycle intermediates, the activity of anaplerotic reactions is pivotal for a high biosynthetic yield. to determine single anaplerotic activities (13) c-metabolic flux analysis ((13) c-mfa) has been extensively used for c. glutamicum, however with different network topologies, inconsistent or poorly determined anaplerotic reaction rates. ...201626375179
overexpression of ppc and lysc to improve the production of 4-hydroxyisoleucine and its precursor l-isoleucine in recombinant corynebacterium glutamicum ssp. lactofermentum.4-hydroxyisoleucine (4-hil) exhibits unique insulinotropic and insulin-sensitizing activities and is an attractive candidate for the treatment of type ii and type i diabetes. in our previous study, l-isoleucine dioxygenase gene (ido) was cloned and overexpressed in an l-isoleucine-producing strain, corynebacterium glutamicum ssp. lactofermentum sn01, and 4-hil was produced from the endogenous l-isoleucine (ile). in this study, ppc and lysc were co-expressed with ido to increase the supply of ile ...201627178798
effect of corynebacterium glutamicum on livestock material burial treatment.in recent years, foot-and-mouth disease has occurred in all parts of the world. the animals with the disease are buried in the ground; therefore, their concentration could affect ground or groundwater. moreover, the complete degradation of carcasses is not a certainty, and their disposal is important to prevent humans, livestock, and the environment from being affected with the disease. the treatment of corynebacterium glutamicum is a feasible method to reduce the risk of carcass decomposition a ...201627160580
recent advances in amino acid production by microbial cells.amino acids have been utilized for the production of foods, animal feeds and pharmaceuticals. after the discovery of the glutamic acid-producing bacterium corynebacterium glutamicum by japanese researchers, the production of amino acids, which are primary metabolites, has been achieved using various microbial cells as hosts. recently, metabolic engineering studies on the rational design of amino acid-producing microbial cells have been successfully conducted. moreover, the technology of systems ...201627151315
increased glucose utilization and cell growth of corynebacterium glutamicum by modifying the glucose-specific phosphotransferase system (pts(glc)) genes.the phosphoenolpyruvate:glucose phosphotransferase system (pts(glc)) is the major pathway of glucose uptake in corynebacterium glutamicum. this study investigated glucose consumption rate, cell growth, and metabolite changes resulting from modification of pts(glc). the classical l-lysine producer c. glutamicum xq-8 exhibited low glucose consumption, cell growth, and l-lysine production rates, whereas these parameters were significantly increased during cultivating on glucose plus maltose, throug ...201627718589
ciprofloxacin triggered glutamate production by corynebacterium glutamicum.corynebacterium glutamicum is a well-studied bacterium which naturally overproduces glutamate when induced by an elicitor. glutamate production is accompanied by decreased 2-oxoglutatate dehydrogenase activity. elicitors of glutamate production by c. glutamicum analyzed to molecular detail target the cell envelope.201627717325
identification of the phd gene cluster responsible for phenylpropanoid utilization in corynebacterium glutamicum.phenylpropanoids as abundant, lignin-derived compounds represent sustainable feedstocks for biotechnological production processes. we found that the biotechnologically important soil bacterium corynebacterium glutamicum is able to grow on phenylpropanoids such as p-coumaric acid, ferulic acid, caffeic acid, and 3-(4-hydroxyphenyl)propionic acid as sole carbon and energy sources. global gene expression analyses identified a gene cluster (cg0340-cg0341 and cg0344-cg0347), which showed increased tr ...201626610800
metabolic engineering of corynebacterium glutamicum for the de novo production of ethylene glycol from glucose.development of sustainable biological process for the production of bulk chemicals from renewable feedstock is an important goal of white biotechnology. ethylene glycol (eg) is a large-volume commodity chemical with an annual production of over 20 million tons, and it is currently produced exclusively by petrochemical route. herein, we report a novel biosynthetic route to produce eg from glucose by the extension of serine synthesis pathway of corynebacterium glutamicum. the eg synthesis is achie ...201626556130
attenuating l-lysine production by deletion of ddh and lyse and their effect on l-threonine and l-isoleucine production in corynebacterium glutamicum.the fermentative production of l-threonine and l-isoleucine with corynebacterium glutamicum is usually accompanied by the by-production of l-lysine, which shares partial biosynthesis pathway with l-threonine and l-isoleucine. since the direct precursor for l-lysine synthesis, diaminopimelate, is a component of peptidoglycan and thus essential for cell wall synthesis, reducing l-lysine by-production could be troublesome. here, a basal strain with eliminated l-lysine production was constructed fro ...201627702487
complete genome sequence of corynebacterium glutamicum cp, a chinese l-leucine producing strain.here, we report the complete genome sequence of corynebacterium glutamicum cp, an industrial l-leucine producing strain in china. the whole genome consists of a circular chromosome and a plasmid. the comparative genomics analysis shows that there are many mutations in the key enzyme coding genes relevant to l-leucine biosynthesis compared to c. glutamicum atcc 13032.201626784991
development of a potential stationary-phase specific gene expression system by engineering of sigb-dependent cg3141 promoter in corynebacterium glutamicum.corynebacterium glutamicum is a non-pathogenic, non-sporulating gram-positive soil bacterium that has been used for the industrial production of various proteins and chemicals. to achieve enhanced and economical production of target molecules, the development of strong auto-inducible promoters is desired, which can be activated without expensive inducers and has significant advantages for industrial-scale use. here, we developed a stationary-phase gene expression system by engineering a sigma fa ...201626782746
rnase iii mediated cleavage of the coding region of mraz mrna is required for efficient cell division in corynebacterium glutamicum.the corynebacterium glutamicum r cgr_1959 gene encodes an endoribonuclease of the rnase iii family. deletion mutant of cgr_1959 (δrnc mutant) showed an elongated cell shape, and presence of several lines on the cell surface, indicating a required of rnase iii for maintaining normal cell morphology in c. glutamicum. the level of mraz mrna was increased, whereas cgr_1596 mrna encoding a putative cell wall hydrolase and ftsex mrna were decreased in the δrnc mutant. the half-life of mraz mrna was si ...201626713407
high-titer biosynthesis of hyaluronic acid by recombinant corynebacterium glutamicum.hyaluronic acid (ha) plays important roles in human tissue system, thus it is highly desirable for various applications, such as in medical, clinic and cosmetic fields. the wild microbial producer of ha, streptococcus, was restricted by its potential pathogens, hence different recombinant hosts are being explored. in this work, we engineered corynebacterium glutamicum, a gras (generally recognized as safe) organism free of exotoxins and endotoxins to produce ha with high titer and satisfied mw . ...201626709615
altered acetylation and succinylation profiles in corynebacterium glutamicum in response to conditions inducing glutamate overproduction.the bacterium corynebacterium glutamicum is utilized during industrial fermentation to produce amino acids such as l-glutamate. during l-glutamate fermentation, c. glutamicum changes the flux of central carbon metabolism to favor l-glutamate production, but the molecular mechanisms that explain these flux changes remain largely unknown. here, we found that the profiles of two major lysine acyl modifications were significantly altered upon glutamate overproduction in c. glutamicum; acetylation de ...201626663479
metabolic engineering corynebacterium glutamicum to produce triacylglycerols.in this study, we metabolically engineered corynebacterium glutamicum to produce triacylglycerols (tags) by completing and constraining a de novo tag biosynthesis pathway. first, the plasmid pz8_tag4 was constructed which allows the heterologous expression of four genes: three (atf1 and atf2, encoding the diacylglycerol acyltransferase; pgpb, encoding the phosphatidic acid phosphatase) to complete the tag biosynthesis pathway, and one gene (tada) for lipid body assembly. second, we applied four ...201626645801
mycothiol peroxidase mpx protects corynebacterium glutamicum against acid stress by scavenging ros.to investigate mycothiol peroxidase (mpx) of corynebacterium glutamicum that is a novel cysgpx family peroxidase using both the mycoredoxin and thioredoxin reducing systems as proton donors for peroxide detoxification and may be involved in the relief of acid stress.201627053080
characterization of aspartate kinase and homoserine dehydrogenase from corynebacterium glutamicum iwj001 and systematic investigation of l-isoleucine biosynthesis.previously we have characterized a threonine dehydratase mutant td(f383v) (encoded by ilva1) and an acetohydroxy acid synthase mutant ahas(p176s, d426e, l575w) (encoded by ilvbn1) in corynebacterium glutamicum iwj001, one of the best l-isoleucine producing strains. here, we further characterized an aspartate kinase mutant ak(a279t) (encoded by lysc1) and a homoserine dehydrogenase mutant hd(g378s) (encoded by hom1) in iwj001, and analyzed the consequences of all these mutant enzymes on amino aci ...201627033538
enhancing pentose phosphate pathway in corynebacterium glutamicum to improve l-isoleucine production.three genes, gnd, pgl, and fbp, relevant to the pentose phosphate pathway (ppp) were overexpressed in corynebacterium glutamicum iwj001, leading to increase of l-isoleucine production. the transcriptional levels of gnd, pgl, and fbp significantly increased in iwj001/pdxw-8-gnd-fbp-pgl. compared with the control strain iwj001/pdxw-8, intracellular nadph/nadp(+) ratios in iwj001/pdxw-8-gnd and iwj001/pdxw-8-gnd-fbp cells grown for 36 h increased threefold and fourfold, respectively, indicating tha ...201627010514
spie interacts with corynebacterium glutamicum whce and is involved in heat and oxidative stress responses.the gene whce in corynebacterium glutamicum positively responds to oxidative and heat stress. to search for proteins that interact with whce, we employed a two-hybrid system with whce as the bait. sequencing analysis of the isolated clones revealed peptide sequences, one of which showed high sequence identity to a hydrophobe/amphiphile efflux-1 family transporter encoded by ncgl1497. the interaction of the ncgl1497-encoded protein with whce in vivo was verified using reporter gene expression by ...201626996627
detoxification of acidic biorefinery waste liquor for production of high value amino acid.the current study evaluates the detoxification of acid pretreatment liquor (apl) using adsorbent (ads 400 & ads 800) or ion-exchange (a-27mp & a-72mp) resins and its potential for amino acid production. the apl is generated as a by-product from the pretreatment of lignocellulosic biomass and is rich monomeric sugars as well as sugar degradation products (fermentation inhibitors) such as furfural and hydroxymethyl furfural (hmf). of the four resins compared, ads 800 removed approximately 85% and ...201626996259
pyruvate kinase deletion as an effective phenotype to enhance lysine production in corynebacterium glutamicum atcc13032: redirecting the carbon flow to a precursor metabolite.various attempts have been made to enhance lysine production in corynebacterium glutamicum. pyruvate kinase (pyk) defect is one of the strategies used to enhance the supply of oxaloacetic acid (oaa), a precursor metabolite for lysine biosynthesis. however, inconsistent effects of this mutation have been reported: positive effects of pyk defect in mutants having phosphoenolpyruvate carboxylase (pepc) desensitized to feedback inhibition by aspartic acid, while negative effects in simple pyk gene ( ...201626983943
metabolic engineering of corynebacterium glutamicum for efficient production of 5-aminolevulinic acid.5-aminolevulinic acid (5-ala) has recently attracted attention for its potential applications in the fields of medicine and agriculture. in this study, corynebacterium glutamicum was firstly engineered for 5-ala production via the c4 pathway. hema encoding 5-aminolevulinic acid synthase from rhodobacter sphaeroides was codon optimized and expressed in c. glutamicum atcc13032, resulting in accumulation of 5-ala. deletion of all known genes responsible for the formation of acetate and lactate furt ...201626616115
efficient production of α-ketoglutarate in the gdh deleted corynebacterium glutamicum by novel double-phase ph and biotin control strategy.production of l-glutamate using a biotin-deficient strain of corynebacterium glutamicum has a long history. the process is achieved by controlling biotin at suboptimal dose in the initial fermentation medium, meanwhile feeding nh4oh to adjust ph so that α-ketoglutarate (α-kg) can be converted to l-glutamate. in this study, we deleted glutamate dehydrogenase (gdh1 and gdh2) of c. glutamicum gkg-047, an l-glutamate overproducing strain, to produce α-kg that is the direct precursor of l-glutamate. ...201626946492
enhancement of 1,5-diaminopentane production in a recombinant strain of corynebacterium glutamicum by tween 40 addition. 201626923131
the extracytoplasmic function σ factor σ(c) regulates expression of a branched quinol oxidation pathway in corynebacterium glutamicum.bacteria modify their expression of different terminal oxidases in response to oxygen availability. corynebacterium glutamicum, a facultative anaerobic bacterium of the phylum actinobacteria, possesses aa3 -type cytochrome c oxidase and cytochrome bd-type quinol oxidase, the latter of which is induced by oxygen limitation. we report that an extracytoplasmic function σ factor, σ(c) , is responsible for the regulation of this process. chromatin immunoprecipitation with microarray analysis detected ...201626789738
monomeric corynebacterium glutamicum n-acetyl glutamate kinase maintains sensitivity to l-arginine but has a lower intrinsic catalytic activity.n-acetyl glutamate kinase (nagk) is a key enzyme in the synthesis of l-arginine, and l-arginine-sensitive nagk typically has hexameric architecture. defining the relationship between this architecture and l-arginine inhibition can provide a foundation to identify the key amino acids involved in the allosteric regulation network of l-arginine. in the present study, the key amino acids in the n-terminal helix (n-helix) of corynebacterium glutamicum (cg) nagk required for hexamer formation were det ...201626512006
implication of ornithine acetyltransferase activity on l-ornithine production in corynebacterium glutamicum.l-ornithine is an intermediate of the l-arginine biosynthetic pathway in corynebacterium glutamicum. the effect of ornithine acetyltransferase (oatase; argj) on l-ornithine production was investigated, and c. glutamicum 1006 was engineered to overproduce l-ornithine as a major product by inactivating regulatory repressor argr gene and overexpressing argj gene. a genome sequence analysis indicated that the argf gene encoding ornithine carbamoyltransferase in c. glutamicum 1006 was mutated, result ...201625630515
effects of phosphoenolpyruvate carboxylase desensitization on glutamic acid production in corynebacterium glutamicum atcc 13032.phosphoenolpyruvate carboxylase (pepc) in corynebacterium glutamicum atcc13032, a glutamic-acid producing actinobacterium, is subject to feedback inhibition by metabolic intermediates such as aspartic acid and 2-oxoglutaric acid, which implies the importance of pepc in replenishing oxaloacetic acid into the tca cycle. here, we investigated the effects of feedback-insensitive pepc on glutamic acid production. a single amino-acid substitution in pepc, d299n, was found to relieve the feedback contr ...201626168906
corynebacterium glutamicum ggtb encodes a functional γ-glutamyl transpeptidase with γ-glutamyl dipeptide synthetic and hydrolytic activity.in this work the role of γ-glutamyl transpeptidase in the metabolism of γ-glutamyl dipeptides produced by corynebacterium glutamicum atcc 13032 was studied. the enzyme is encoded by the gene ggtb (cg1090) and synthesized as a 657 amino acids long preprotein. gamma-glutamyl transpeptidase activity was found to be associated with intact cells of c. glutamicum and was abolished upon deletion of ggtb. bioinformatic analysis indicated that the enzyme is a lipoprotein and is attached to the outer side ...201626528625
regulons of global transcription factors in corynebacterium glutamicum.corynebacterium glutamicum, a high gc content gram-positive soil bacterium in actinobacteria, has been used for the industrial production of amino acids and engineered to produce various compounds, including polymer building blocks and biofuels. since its genome sequence was first published, its versatile metabolic pathways and their genetic components and regulatory mechanisms have been extensively studied. previous studies on transcriptional factors, including two-component systems and σ facto ...201626496920
metabolic engineering of a laboratory-evolved thermobifida fusca muc strain for malic acid production on cellulose and minimal treated lignocellulosic biomass.malic acid is mainly used as an acidulant and taste enhancer in the beverage and food industry. previously, a mutant strain thermobifida fusca muc, obtained by adaptive evolution was found to accumulate malic acid on cellulose with low yield. in this study, the malic acid synthesis pathway in t. fusca muc was confirmed to be from phosphoenolpyruvate to oxaloacetate, followed by reduction of oxaloacetate to malate. to increase the yield of malic acid by the muc strain significantly, the carbon fl ...201726439318
boosting anaplerotic reactions by pyruvate kinase gene deletion and phosphoenolpyruvate carboxylase desensitization for glutamic acid and lysine production in corynebacterium glutamicum.in the 1980s, shiio and coworkers demonstrated using random mutagenesis that the following three phenotypes were effective for boosting lysine production by corynebacterium glutamicum: (1) low-activity-level citrate synthase (cs(l)), (2) phosphoenolpyruvate carboxylase (pepc) resistant to feedback inhibition by aspartic acid (pepc(r)), and (3) pyruvate kinase (pyk) deficiency. here, we reevaluated these phenotypes and their interrelationship in lysine production using recombinant dna techniques. ...201727872961
mutations in mure, the essential udp-n-acetylmuramoylalanyl-d-glutamate 2,6-diaminopimelate ligase of corynebacterium glutamicum: effect on l-lysine formation and analysis of systemic consequences.to explore systemic effects of mutations in the udp-n-acetylmuramoylalanyl-d-glutamate 2,6-diaminopimelate ligase (mure) of corynebacterium glutamicum, that leads to extracellular l-lysine accumulation by this bacterium.201727783176
a novel synthetic pathway enables microbial production of polyphenols independent from the endogenous aromatic amino acid metabolism.numerous plant polyphenols have potential applications as pharmaceuticals or nutraceuticals. stilbenes and flavonoids as most abundant polyphenols are synthesized from phenylpropanoids, which are exclusively derived from aromatic amino acids in nature. several microorganisms were engineered for the synthesis of biotechnologically interesting plant polyphenols; however, low activity of heterologous ammonia lyases, linking endogenous microbial aromatic amino acid biosynthesis to phenylpropanoid sy ...201727936616
glutamate fermentation-2: mechanism of l-glutamate overproduction in corynebacterium glutamicum.the nonpathogenic coryneform bacterium, corynebacterium glutamicum, was isolated as an l-glutamate-overproducing microorganism by japanese researchers and is currently utilized in various amino acid fermentation processes. l-glutamate production by c. glutamicum is induced by limitation of biotin and addition of fatty acid ester surfactants and β-lactam antibiotics. these treatments affect the cell surface structures of c. glutamicum. after the discovery of c. glutamicum, many researchers have i ...201727913829
discovery and history of amino acid fermentation.there has been a strong demand in japan and east asia for l-glutamic acid as a seasoning since monosodium glutamate was found to present umami taste in 1907. the discovery of glutamate fermentation by corynebacterium glutamicum in 1956 enabled abundant and low-cost production of the amino acid, creating a large market. the discovery also prompted researchers to develop fermentative production processes for other l-amino acids, such as lysine. currently, the amino acid fermentation industry is so ...201727909736
comparative analysis of the corynebacterium glutamicum transcriptome in response to changes in dissolved oxygen levels.the dissolved oxygen (do) level of a culture of corynebacterium glutamicum (c. glutamicum) in a bioreactor has a significant impact on the cellular redox potential and the distribution of energy and metabolites. in this study, to gain a deeper understanding of the effects of do on the metabolism of c. glutamicum, we sought to systematically explore the influence of different do concentrations on genetic regulation and metabolism through transcriptomic analysis. the results revealed that after 20 ...201727844170
functional architecture and global properties of the corynebacterium glutamicum regulatory network: novel insights from a dataset with a high genomic coverage.corynebacterium glutamicum is a gram-positive, anaerobic, rod-shaped soil bacterium able to grow on a diversity of carbon sources like sugars and organic acids. it is a biotechnological relevant organism because of its highly efficient ability to biosynthesize amino acids, such as l-glutamic acid and l-lysine. here, we reconstructed the most complete c. glutamicum regulatory network to date and comprehensively analyzed its global organizational properties, systems-level features and functional a ...201727829123
improvement of succinate production by release of end-product inhibition in corynebacterium glutamicum.succinate is a renewable-based platform chemical that may be used to produce a wide range of chemicals including 1,4-butanediol, tetrahydrofurane, and γ-butyrolactone. however, industrial fermentation of organic acids is often subject to end-product inhibition, which significantly retards cell growth and limits metabolic activities and final productivity. in this study, we report the development of metabolically engineered corynebacterium glutamicum for high production of succinate by release of ...201728232033
click-chemistry approach to study mycoloylated proteins: evidence for porb and porc porins mycoloylation in corynebacterium glutamicum.protein mycoloylation is a recently identified, new form of protein acylation. this post-translational modification consists in the covalent attachment of mycolic acids residues to serine. mycolic acids are long chain, α-branched, β-hydroxylated fatty acids that are exclusively found in the cell envelope of corynebacteriales, a bacterial order that includes important genera such as mycobacterium, nocardia or corynebacterium. so far, only 3 mycoloylated proteins have been identified: pora, porh a ...201728199365
comparative analysis of corynebacterium glutamicum genomes: a new perspective for the industrial production of amino acids.corynebacterium glutamicum is a non-pathogenic bacterium widely used in industrial amino acid production and metabolic engineering research. although the genome sequences of some c. glutamicum strains are available, comprehensive comparative genome analyses of these species have not been done. six wild type c. glutamicum strains were sequenced using next-generation sequencing technology in our study. together with 20 previously reported strains, we present a comprehensive comparative analysis of ...201728198668
cofactor recycling for co-production of 1,3-propanediol and glutamate by metabolically engineered corynebacterium glutamicum.production of 1,3-propanediol (1,3-pdo) from glycerol is a promising route toward glycerol biorefinery. however, the yield of 1,3-pdo is limited due to the requirement of nadh regeneration via glycerol oxidation process, which generates large amounts of undesired byproducts. glutamate fermentation by corynebacterium glutamicum is an important oxidation process generating excess nadh. in this study, we proposed a novel strategy to couple the process of 1,3-pdo synthesis with glutamate production ...201728176878
structural basis for redox sensitivity in corynebacterium glutamicum diaminopimelate epimerase: an enzyme involved in l-lysine biosynthesis.diaminopimelate epimerase (dapf) is one of the crucial enzymes involved in l-lysine biosynthesis, where it converts l,l-diaminopimelate (l,l-dap) into d,l-dap. dapf is also considered as an attractive target for the development of antibacterial drugs. here, we report the crystal structure of dapf from corynebacterium glutamicum (cgdapf). structures of cgdapf obtained under both oxidized and reduced conditions reveal that the function of cgdapf is regulated by redox-switch modulation via reversib ...201728176858
fermentative production of l-pipecolic acid from glucose and alternative carbon sources.corynebacterium glutamicum is used for the million-ton scale production of amino acids and has recently been engineered for production of the cyclic non-proteinogenic amino acid l-pipecolic acid (l-pa). in this synthetic pathway l-lysine was converted to l-pa by oxidative deamination, dehydration and reduction by l-lysine 6-dehydrogenase (deaminating) from silicibacter pomeroyi and pyrroline 5-carboxylate reductase from c. glutamicum. however, production of l-pa occurred as by-product of l-lysin ...201728169491
l-lysine production by bacillus methanolicus: genome-based mutational analysis and l-lysine secretion engineering.bacillus methanolicus is a methylotrophic bacterium with an increasing interest in academic research and for biotechnological applications. this bacterium was previously applied for methanol-based production of l-glutamate, l-lysine and the five-carbon diamine cadaverine by wild type, classical mutant and recombinant strains. the genomes of two different l-lysine secreting b. methanolicus classical mutant strains, noa2#13a52-8a66 and m168-20, were sequenced. we focused on mutational mapping in g ...201728163092
modular pathway engineering of corynebacterium glutamicum to improve xylose utilization and succinate production.xylose-negative corynebacterium glutamicum has been engineered to utilize xylose as the sole carbon source via either the xylose isomerase (xi) pathway or the weimberg pathway. heterologous expression of xylose isomerase and overexpression of a gene encoding for xylulose kinase enabled efficient xylose utilization. in this study, we show that two functionally-redundant transcriptional regulators (gntr1 and gntr2) present on xylose repress the pentose phosphate pathway genes. for efficient xylose ...201728153765
comprehensive and accurate tracking of carbon origin of lc-tandem mass spectrometry collisional fragments for (13)c-mfa.in recent years the benefit of measuring positionally resolved (13)c-labeling enrichment from tandem mass spectrometry (ms/ms) collisional fragments for improved precision of (13)c-metabolic flux analysis ((13)c-mfa) has become evident. however, the usage of positional labeling information for (13)c-mfa faces two challenges: (1) the mass spectrometric acquisition of a large number of potentially interfering mass transitions may hamper accuracy and sensitivity. (2) the positional identity of carb ...201728116490
novel chromosome organization pattern in actinomycetales-overlapping replication cycles combined with diploidy.bacteria regulate chromosome replication and segregation tightly with cell division to ensure faithful segregation of dna to daughter generations. the underlying mechanisms have been addressed in several model species. it became apparent that bacteria have evolved quite different strategies to regulate dna segregation and chromosomal organization. we have investigated here how the actinobacterium corynebacterium glutamicum organizes chromosome segregation and dna replication. unexpectedly, we fo ...201728588128
metabolic design of corynebacterium glutamicum for production of l-cysteine with consideration of sulfur-supplemented animal feed.l-cysteine is a valuable sulfur-containing amino acid widely used as a nutrition supplement in industrial food production, agriculture, and animal feed. however, this amino acid is mostly produced by acid hydrolysis and extraction from human or animal hairs. in this study, we constructed recombinant corynebacterium glutamicum strains that overexpress combinatorial genes for l-cysteine production. the aims of this work were to investigate the effect of the combined overexpression of serine acetyl ...201728560868
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