Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| identification of a wzy polymerase required for group iv capsular polysaccharide and lipopolysaccharide biosynthesis in vibrio vulnificus. | the estuarine bacterium vibrio vulnificus is a human and animal pathogen. the expression of capsular polysaccharide (cps) is essential for virulence. we used a new mini-tn10 delivery vector, pnktxi-scei, to generate a mutant library and identify genes essential for cps biosynthesis. twenty-one acapsular mutants were isolated, and the disrupted gene in one mutant, coding for a polysaccharide polymerase (wzy), is described here. a weca gene initiating glycosyltransferase was among the genes identi ... | 2007 | 17923517 |
| expression and immunogenicity analysis of two iron-regulated outer membrane proteins of vibrio parahaemolyticus. | genes of two iron-regulated outer membrane proteins of vibrio parahaemolyticus zj2003, a pathogenic strain isolated from large yellow croaker (pseudosciaena crocea), psua and pvua, were cloned and expressed as n-terminal his(6)-tagged proteins in escherichia coli bl(21)(de(3)). the recombinant fusion proteins were purified with nickel chelate affinity chromatography. to analyze the immunogenicity of the proteins, groups of large yellow croaker were immunized with the purified recombinant psua, p ... | 2007 | 17928925 |
| role of iron in human serum resistance of the clinical and environmental vibrio vulnificus genotypes. | we recently reported a simple pcr procedure that targets a sequence variation of the virulence-correlated gene locus vcg. it was found that 90% of all clinical isolates possessed the vcgc sequence variant, while 93% of all environmental isolates possessed the vcge sequence variant. here we report that the clinical genotype of vibrio vulnificus is significantly better able to survive in human serum than is the environmental genotype. the presence of a siderophore-encoding gene, viub, influenced s ... | 2007 | 17933938 |
| survival case of vibrio vulnificus infection with multiple endocrine neoplasia type i. | a 35-year-old female with malabsorption syndrome who underwent a pancreatoduodenectomy for multiple endocrine adenomatosis 13 years prior was admitted to our hospital with diarrhea, general fatigue, high fever, and eruption in the lower legs. the patient had consumed raw shrimp a few days before onset and presented systemic inflammatory response syndrome at the time of hospitalization. vibrio vulnificus was isolated from a blood culture performed before admission to the intensive care unit. we e ... | 2007 | 17974078 |
| phospholipase a as a potent virulence factor of vibrio vulnificus. | vibrio vulnificus infection has attracted special interest because of its high mortality rate. however, the identification of its major pathogenic determinant still remains obscure. in this study, a cytolysin-negative mutant strain of v. vulnificus cvd707 was used to determine the role of phospholipase a (pla) in the pathogenesis of this bacterial infection. the mutant strain caused the lysis of erythrocytes in vitro and elevated plasma hemoglobin during the infection in mice. both the hemolytic ... | 2007 | 17982702 |
| vibrio infections in louisiana: twenty-five years of surveillance 1980-2005. | a total of 1,007 vibrio infections were reported to the infectious disease epidemiology department at the louisiana office of public heath, between 1980 and 2005. the most common were vibrio vulnificus (257 infections), vibrio parahemolyticus (249 infections), and vibrio cholerae non o1 (200 cases). other species were much less common. vibrio vulnificus infections, which are associated with consumption of raw seafood (particularly oysters) or contact with sea water, and severe immuno-suppression ... | 2007 | 17987958 |
| identification of vibrio isolates by a multiplex pcr assay and rpob sequence determination. | vibrio, a diverse genus of aquatic bacteria, currently includes 72 species, 12 of which occur in human clinical samples. of these 12, three species--vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus-account for the majority of vibrio infections in humans. rapid and accurate identification of vibrio species has been problematic because phenotypic characteristics are variable within species and biochemical identification requires 2 or more days to complete. to facilitate the identifi ... | 2007 | 17093013 |
| vibrio extracellular protease with prothrombin activation and fibrinolytic activities. | a 36 kda extracellular metalloprotease (designated to as vep-mo6) was purified and characterized from vibrio vulnificus sp. strain mo6 24/0. vep-mo6 cleaved azocasein and a few other proteins such as prothrombin, plasminogen, fibrinogen and factor xa, which are associated with the blood coagulation pathway. the enzyme activity of vep-mo6 was inhibited by edta, which was reversed by the addition of excess divalent cations. vep-mo6 showed little or no activity toward various chromogenic substrates ... | 2007 | 17143560 |
| ferrophilic characteristics of vibrio vulnificus and potential usefulness of iron chelation therapy. | we determined the ferrophilic characteristics of vibrio vulnificus to evaluate the potential usefulness of iron chelation therapy for the prevention of v. vulnificus infection. readily available non-transferrin-bound iron (ntbi) is required for the initiation of v. vulnificus growth under in vitro iron-limited conditions and human ex vivo conditions. ntbi aided efficient transferrin-bound iron (tbi) use by v. vulnificus, and the vulnibactin-mediated iron-uptake system was expressed after bacteri ... | 2007 | 17152012 |
| rapid detection of vibrio species using liquid microsphere arrays and real-time pcr targeting the ftsz locus. | the development of rapid and sensitive molecular techniques for the detection of vibrio species would be useful for the surveillance of sporadic infections and management of major outbreaks. comparative sequence analysis of the ftsz gene in the predominant vibrio species that cause human disease revealed distinct alleles for each examined species, including vibrio cholerae, vibrio parahaemolyticus and vibrio vulnificus. light upon extension (lux) real-time pcr assays were developed to target the ... | 2007 | 17172518 |
| structural basis for sequence-dependent dna cleavage by nonspecific endonucleases. | nonspecific endonucleases hydrolyze dna without sequence specificity but with sequence preference, however the structural basis for cleavage preference remains elusive. we show here that the nonspecific endonuclease cole7 cleaves dna with a preference for making nicks after (at 3'o-side) thymine bases but the periplasmic nuclease vvn cleaves dna more evenly with little sequence preference. the crystal structure of the 'preferred complex' of the nuclease domain of cole7 bound to an 18 bp dna with ... | 2007 | 17175542 |
| osborn wave in hypothermia from vibrio vunificus sepsis unrelated to exposure. | a 58 year old man with history of cirrhosis presented with vibrio vunificus sepsis. the patient developed multiorgan failure despite appropriate antibiotic therapy and fluid resuscitation. the patient developed moderate hypothermia. electrocardiography showed osborn wave. osborn wave is commonly seen in accidental hypothermia. although sepsis is known to cause hypothermia, osborn wave in sepsis is not widely appreciated. | 2007 | 17046085 |
| rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time pcr. | buoyant density gradient centrifugation has been used to separate bacteria from complex food matrices, as well as to remove compounds that inhibit rapid detection methods, such as pcr, and to prevent false-positive results due to dna originating from dead cells. applying a principle of buoyant density gradient centrifugation, we developed a method for rapid separation and concentration following filtration and low- and high-speed centrifugation, as well as flotation and sedimentation buoyant den ... | 2007 | 17056684 |
| serological and molecular characteristics of vibrio vulnificus biotype 3: evidence for high clonality. | vibrio vulnificus biotype 3 has been implicated as the causative pathogen of an ongoing disease outbreak that erupted in israel in 1996. recent work based on multi-locus sequence typing (mlst) showed that v. vulnificus biotype 3 is genetically homogeneous. the aim of this study was to investigate the existence of subpopulations within this homogeneous biotype by characterizing the surface antigens and analysing the sequence diversity of selected outer-membrane protein (omp)-encoding genes. rabbi ... | 2007 | 17322205 |
| shewanella infection in decompensated liver disease: a septic case. | shewanella species are an unusual cause of disease in humans. however, reports of shewanella infections have been increasing, and hepatobiliary disease has been proposed as a predisposing factor following a critical course. we report the first japanese septic case of decompensated liver disease in which this bacterium acted as a definite pathogen. a 67-year-old japanese man with primary sclerosing cholangitis was admitted to our hospital complaining of fever, general fatigue, pain, and a rash on ... | 2007 | 17322999 |
| occurrence of vibrio spp. in blue mussels (mytilus edulis) from the german wadden sea. | the present study aimed at determining the contamination of blue mussels grown in germany with relevant bacterial organisms. seasonal variations were examined. for that purpose, mussel samples were taken regularly between june 2004 and may 2005 in seven shellfish-growing areas of the german wadden sea. a total of 90 samples were analysed. the analysis included the compulsory microbiological parameters escherichia coli and salmonella spp., as well as the determination of the contamination with vi ... | 2007 | 17331605 |
| necrotizing soft-tissue infections and sepsis caused by vibrio vulnificus compared with those caused by aeromonas species. | vibrio and aeromonas species, which can cause necrotizing fasciitis and primary septicemia, are members of the vibrionaceae family and thrive in aquatic environments. because the clinical symptoms and signs of necrotizing fasciitis and sepsis caused by these two bacteria are similar, the purposes of this study were to describe the clinical characteristics of vibrio vulnificus and aeromonas infections, to analyze the risk factors for death, and to compare the effects of surgical treatment on the ... | 2007 | 17332113 |
| refined medium for direct isolation of vibrio vulnificus from oyster tissue and seawater. | we have developed a new medium for the direct isolation of vibrio vulnificus from water and oyster samples. the medium was shown in laboratory and field studies to be highly selective without providing preferential isolation of either v. vulnificus genotype. | 2007 | 17337558 |
| [studies on vibrio vulnificus infection: molecular epidemiology of environment-derived strains and clinical isolates]. | to clarify the route and source of vibrio vulnificus infection, we conducted molecular epidemiological investigation by dna analysis of 355 environmental isolates (seawater-derived strain: 86, sea mud-derived strain:36, and oyster-derived strain: 233) and 65 human clinical isolates, for a total of 420 isolates, using pulse field gel electrophoresis (pfge), with the following results. 1. when dna was cleaved with 2 enzymes, not i and sfi i, and subjected to pfge, not i dna interpretation was 76.9 ... | 2007 | 17338316 |
| comment on "vibrio vulnificus and v. parahaemolyticus necrotising fasciitis in fishermen visiting an estuarine tropical northern australia location". | 2007 | 17350104 | |
| chromosomal toxin-antitoxin loci can diminish large-scale genome reductions in the absence of selection. | superintegrons (sis) are chromosomal genetic elements containing assemblies of genes, each flanked by a recombination sequence (attc site) targeted by the integron integrase. sis may contain hundreds of attc sites and intrinsic instability is anticipated; yet sis are remarkably stable. this implies that either selective pressure maintains the genes or mechanisms exist which favour their persistence in the absence of selection. toxin/antitoxin (ta) systems encode a stable toxin and a specific, un ... | 2007 | 17367382 |
| the pyrh gene of vibrio vulnificus is an essential in vivo survival factor. | we have suggested an important role of the pyrh gene during the infectious process of vibrio vulnificus. previously, we have identified 12 genes expressed preferentially during human infections by using in vivo-induced antigen technology. among the in vivo-expressed genes, pyrh encodes ump kinase catalyzing ump phosphorylation. introduction of a deletion mutation to the pyrh gene was lethal to v. vulnificus, and an insertional mutant showed a high frequency of curing. we constructed a site-direc ... | 2007 | 17371864 |
| thermal factors influencing detection of vibrio vulnificus using real-time pcr. | five thermal factors, including initial denaturation temperature, cycling denaturation temperature, annealing temperature, extension temperature and the temperature at which the intensity of the fluorescent signal is read, were evaluated for their effects on the detection of vibrio vulnificus via real-time pcr. fluorescent signal detection after extension was set between the tm value of the primer-dimers (79 degrees c) and that of the pcr target amplicons (84 degrees c). this effectively elimina ... | 2007 | 17383036 |
| growth response of vibrio cholerae and other vibrio spp. to cyanobacterial dissolved organic matter and temperature in brackish water. | environmental control of growth and persistence of vibrios in aquatic environments is poorly understood even though members of the genus vibrio are globally important pathogens. to study how algal-derived organic matter and temperature influenced the abundance of different vibrio spp., baltic sea microcosms inoculated with vibrio cholerae, vibrio vulnificus, vibrio parahaemolyticus, vibrio alginolyticus and native bacterioplankton, were exposed to different temperatures (12-25 degrees c) and ame ... | 2007 | 17386033 |
| surface properties of vibrio vulnificus. | vibrio vulnificus adheres to a diverse range of surfaces, ranging from the chitinous exoskeleton of mollusks to human tissue. to determine whether environmental and human clinical isolates exhibit different adhesion traits, we studied the ability of 10 environmental isolates and 10 clinical isolates to adhere to human epithelial cells and hydrocarbons with log p values ranging from 3.1 to 8.2. | 2007 | 17397482 |
| recovery and detection of vibrio vulnificus during cold storage. | different cultural techniques and molecular methods for the detection of vibrio vulnificus during cold storage in a model broth system were compared. two strains of v. vulnificus were grown to stationary phase and inoculated (10(6) cfu/ml) into tryptic soy broth with 2% sodium chloride (tsbn2) or artificial seawater (asw), both pre-chilled to 5 degrees c. these were stored for 10 days, with sub-sampling conducted at time 0 and every 2 days thereafter. each subsample was plated, by both pour and ... | 2007 | 17418319 |
| the hlyu protein is a positive regulator of rtxa1, a gene responsible for cytotoxicity and virulence in the human pathogen vibrio vulnificus. | vibrio vulnificus is an opportunistic human pathogen that preferentially infects compromised iron-overloaded patients, causing a fatal primary septicemia with very rapid progress, resulting in a high mortality rate. in this study we determined that the hlyu protein, a virulence factor in v. vulnificus cmcp6, up-regulates the expression of vv20479, a homologue of the vibrio cholerae rtx (repeats in toxin) toxin gene that we named rtxa1. this gene is part of an operon together with two other open ... | 2007 | 17438022 |
| ligand-induced folding of the adenosine deaminase a-riboswitch and implications on riboswitch translational control. | by using a structure-based fluorescence spectroscopic approach, we have examined the folding of an adenine-responsive riboswitch that regulates translation initiation. we observed adaptive recognition of the ligand for the aptamer domain of adenosine deaminase (add) mrna from vibrio vulnificus, and revealed pronounced conformational changes even in the preorganized loop-loop region that is distant from the binding site. importantly, the full-length riboswitch domain, which has a potential transl ... | 2007 | 17440909 |
| synergistic antimicrobial effect of cefotaxime and minocycline on proinflammatory cytokine levels in a murine model of vibrio vulnificus infection. | vibrio vulnificus causes primary bacteremia and necrotizing wound infection, leading to high morbidity and mortality in humans. this study aimed to evaluate the antimicrobial effect of cefotaxime and minocycline on proinflammatory cytokine levels in a murine model of v. vulnificus infection. | 2007 | 17446960 |
| production of vibrio vulnificus metalloprotease vvpe begins during the early growth phase: usefulness of gelatin-zymography. | recent studies have demonstrated that expression of the vvpe gene begins during the early growth phase albeit at low levels. however, we found that the traditional protease assay method that is used to measure caseinolytic activity in culture supernatants is not suitable for the measurement of extracellular vvpe that is produced at low levels during the early growth phase. by using gelatin-zymography in place of the protease assay, we could specifically detect only vvpe of several proteases prod ... | 2007 | 17467832 |
| a short-chain dehydrogenase/reductase from vibrio vulnificus with both blue fluorescence and oxidoreductase activity. | a dual function blue fluorescent protein from vibrio vulnificus is also an nadph-dependent oxidoreductase, rendering it a useful tool for biophysical studies. | 2007 | 17476406 |
| identification and characterization of the vibrio vulnificus rtxa essential for cytotoxicity in vitro and virulence in mice. | a mutant exhibiting decreased cytotoxic activity toward int-407 intestinal epithelial cells and carrying a mutation in the rtx gene cluster that consists of rtxca and rtxbde operons was screened from a library of v. vulnificus mutants. the functions of the rtxa gene, assessed by constructing an isogenic mutant and evaluating its phenotypic changes, demonstrated that rtxa is essential for the virulence of v. vulnificus in mice as well as in tissue cultures. | 2007 | 17483800 |
| identification of quorum sensing-related regulons in vibrio vulnificus by two-dimensional gel electrophoresis and differentially displayed reverse transcriptase pcr. | vibrio vulnificus is thought to employ a quorum-sensing system to control the expression of a global gene. in this study, proteomes and transcriptomes of a lacz null mutant, vvsr delta z, and a luxs-smcr double mutant, vvsr delta zsr, were compared with the parent strain, vvar, by means of two-dimensional gel electrophoresis (2d-page) and differentially displayed reverse transcriptase pcr (ddrt-pcr). 2d-page analysis showed that 36 protein spots were differentially expressed, 14 of which have be ... | 2007 | 17506728 |
| phenotypic and genotypic characterization of a new fish-virulent vibrio vulnificus serovar that lacks potential to infect humans. | vibrio vulnificus is a bacterial species that is virulent for humans and fish. human isolates are classified into biotypes 1 and 3 (bt1 and bt3) and fish isolates into biotype 2 (bt2). however, a few human infections caused by bt2 isolates have been reported worldwide (zoonosis). these bt2 human isolates belong to serovar e (sere), which is also present in diseased fish. the aim of the present work was to characterize a new bt2 serovar [serovar a (sera)], which emerged in the european fish-farmi ... | 2007 | 17526849 |
| [isolation and identification of species from the genera aeromonas, vibrio, and plesiomonas from extraintestinal samples in cuba]. | extraintestinal infections caused by the genera aeromonas, vibrio and plesiomonas have high morbidity and mortality rates in different areas of world. from january 2002 to december 2003, the national reference laboratory for acute diarrhoeal diseases of the pedro kourí tropical medicine institute received 95 gram-negative, facultative anaerobic, oxidase positive bacilli strains from different extraintestinal specimen (blood, ear exudates, infected wounds, conjunctive exudates, urine, and cathete ... | 2007 | 17554439 |
| role of type iv pilins in persistence of vibrio vulnificus in crassostrea virginica oysters. | vibrio vulnificus is part of the natural estuarine microflora and accumulates in shellfish through filter feeding. it is responsible for the majority of seafood-associated fatalities in the united states mainly through consumption of raw oysters. previously we have shown that a v. vulnificus mutant unable to express pild, the type iv prepilin peptidase, does not express pili on the surface of the bacterium and is defective in adherence to human epithelial cells (r. n. paranjpye, j. c. lara, j. c ... | 2007 | 17557854 |
| gene expression and localization of the epinecidin-1 antimicrobial peptide in the grouper (epinephelus coioides), and its role in protecting fish against pathogenic infection. | epinecidin-1 is an antimicrobial peptide and plays a vital role in protecting fish against pathogenic infection. as a mimic of a grouper epinecidin-1 peptide, it has tertiary structures that closely resemble those of pleurocidin found in the winter flounder (pleuronectes americanus). the tissue-specific, lipopolysaccharide (lps)-stimulation-specific, and poly(i):poly(c)-stimulation-specific expressions of the grouper (epinephelus coioides) epinecidin-1 antimicrobial peptide were determined using ... | 2007 | 17570764 |
| catecholamine-induced stimulation of growth in vibrio species. | to evaluate the effect of norepinephrine (ne) and related compounds on the growth of bacteria, we have examined the effect of the neuroendocrine hormone ne and related compounds on the growth of vibrio parahaemolyticus and other human-pathogenic vibrio species (vibrio cholerae, vibrio vulnificus, and vibrio mimicus). | 2007 | 17576228 |
| vibrio vulnificus damages macrophages during the early phase of infection. | vibrio vulnificus is an estuarine bacterium that can cause primary septicemia as well as serious wound infections. generally, clinical isolates have a high lethal effect compared with environmental isolates. however, little is known about the mechanisms by which v. vulnificus causes disease. in this study, we compared the pathogenicity of a clinical isolate, strain m2799, with that of an environmental isolate, strain jcm3731. the clinical isolate showed 100 times higher lethality in mice than th ... | 2007 | 17591793 |
| pathogenic bacteria associated with oysters (crassostrea brasiliana) and estuarine water along the south coast of brazil. | oysters and estuarine water samples were collected monthly, from june 1998 to march 1999, in the cananéia estuary, on the south coast of são paulo, brazil, and analyzed for bacterial hazards with and without depuration in filtered estuarine water. aeromonas spp., plesiomonas shigelloides, vibrio cholerae o1, vibrio parahaemolyticus, and vibrio vulnificus were counted in oyster samples using the most probable number (mpn) and their presence verified in the surrounding estuarine water samples. the ... | 2007 | 17613090 |
| rapid and specific identification of 5 human pathogenic vibrio species by multiplex polymerase chain reaction targeted to dnaj gene. | a multiplex polymerase chain reaction (pcr) method, specifically designed for application in routine diagnostic laboratories, was developed for identifying 5 human pathogen vibrio species: vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio mimicus, and vibrio alginolyticus. this assay directed toward the dnaj gene was tested on a total of 355 strains representing 13 vibrio species and 17 non-vibrio species. specific pcr fragments were produced in isolates belonging to the 5 targ ... | 2007 | 17614235 |
| emergence of a virulent clade of vibrio vulnificus and correlation with the presence of a 33-kilobase genomic island. | vibrio vulnificus is a ubiquitous inhabitant of the marine coastal environment, and an important pathogen of humans. we characterized a globally distributed sample of environmental isolates from a range of habitats and hosts and compared these with isolates recovered from cases of human infection. multilocus sequence typing data using six housekeeping genes divided 63 of the 67 isolates into the two main lineages previously noted for this species, and this division was also confirmed using the 1 ... | 2007 | 17616611 |
| inhibition of vibrio biofilm formation by a marine actinomycete strain a66. | china remains by far the largest aquaculture producer in the world. however, biofilms formed by pathogenic vibrio strains pose serious problems to marine aquaculture. to provide a strategy for biofilm prevention, control, and eradication, extracts from 88 marine actinomycetes were screened. thirty-five inhibited the biofilm formation of vibrio harveyi, vibrio vulnificus, and vibrio anguillarum at a concentration of 2.5% (v/v). thirty-three of the actinomycete extracts dispersed the mature biofil ... | 2007 | 17624525 |
| vibrio vulnificus infection in são paulo, brazil: case report and literature review. | non cholera vibrio may cause conjunctivitis, wound infection, gastroenteritis and serious sepsis. transmission to men is through contact with skin, mucosa or wounds exposed to marine water, and consumption of certain barely cooked or raw seafood, more frequently in the summer. this is one of the first cases of severe infection related to vibrio vulnificus described in brazil. the patient was an old man, who ingested seafood in guarujá, a seashore city near são paulo, 3 days before hospitalizatio ... | 2007 | 17625784 |
| phylogenetic diversity and the structural basis of substrate specificity in the beta/alpha-barrel fold basic amino acid decarboxylases. | the beta/alpha-barrel fold type basic amino acid decarboxylases include eukaryotic ornithine decarboxylases (odc) and bacterial and plant enzymes with activity on l-arginine and meso-diaminopimelate. these enzymes catalyze essential steps in polyamine and lysine biosynthesis. phylogenetic analysis suggests that diverse bacterial species also contain odc-like enzymes from this fold type. however, in comparison with the eukaryotic odcs, amino acid differences were identified in the sequence of the ... | 2007 | 17626020 |
| classification of response regulators based on their surface properties. | the two-component signal transduction system is a ubiquitous signaling module present in most prokaryotic and some eukaryotic systems. two conserved components, a histidine protein kinase (hpk) protein and a response regulator (rr) protein, function as a biological switch, sensing and responding to changes in the environment, thereby eliciting a specific response. extensive studies have classified the hpk and rr proteins using primary sequence characteristics, domain identity, domain organizatio ... | 2007 | 17628138 |
| vibrio vulnificus ilpa-induced cytokine production is mediated by toll-like receptor 2. | vibrio vulnificus is a pathogenic bacterium causing primary septicemia, which follows a classical septic shock pathway, including an overwhelming inflammatory cytokine response. in this study, we identified a putative lipoprotein of v. vulnificus, encoded by the ilpa gene, as one of the surface proteins that specifically reacted with the antibodies raised against outer membrane proteins of v. vulnificus. using a mutant v. vulnificus in which its ilpa gene was knocked out, we found that ilpa is i ... | 2007 | 17640874 |
| the n-terminal propeptide of vibrio vulnificus extracellular metalloprotease is both an inhibitor of and a substrate for the enzyme. | vibrio vulnificus, a marine bacterium capable of causing wound infection and septicemia, secretes a 45-kda metalloprotease (vep) with many biological activities. the precursor of vep consists of four regions: a signal peptide, an n-terminal propeptide (npp), a c-terminal propeptide, and the mature protease. two forms of vep-vep-45, which contains the mature protease plus the c-terminal propeptide, and vep-34, which contains only the mature protease-were expressed in escherichia coli and purified ... | 2007 | 17644589 |
| screening and evaluation of probiotics as a biocontrol agent against pathogenic vibrios in marine aquaculture. | the present work aims at finding potential probionts from marine sources as a biocontrol agent against pathogenic vibrio species in shrimp larval culture. | 2007 | 17651222 |
| vibrio vulnificus typing based on simple sequence repeats: insights into the biotype 3 group. | vibrio vulnificus is an opportunistic, highly invasive human pathogen with worldwide distribution. v. vulnificus strains are commonly divided into three biochemical groups (biotypes), most members of which are pathogenic. simple sequence repeats (ssr) provide a source of high-level genomic polymorphism used in bacterial typing. here, we describe the use of variations in mutable ssr loci for accurate and rapid genotyping of v. vulnificus. an in silico screen of the genomes of two v. vulnificus st ... | 2007 | 17652479 |
| [pathogenic vibrios in oysters (crassostrea rhizophorae) served at restaurants in rio de janeiro: a public health warning]. | forty oyster samples (crassostrea rhizophorae) served raw in 15 restaurants in the city of rio de janeiro were evaluated in order to investigate the presence of vibrio spp. the oyster samples were analyzed and subjected to enrichment in alkaline peptone water with the addition of 1 and 3% nacl and incubated at 37 degrees c for 24 hours. following this, the cultures were seeded onto thiosulfate citrate bile sucrose agar (tcbs) and the suspected colonies were subjected to biochemical characterizat ... | 2007 | 17653465 |
| necrotizing fasciitis caused by vibrio vulnificus: epidemiology, clinical findings, treatment and prevention. | necrotizing fasciitis is a soft-tissue infection with a high risk of fatality. infection with vibrio vulnificus can lead to development of necrotizing fasciitis and primary septicemia, and occurs mostly in immunocompromised host-associated diseases such as hepatic disease, diabetes mellitus, chronic renal insufficiency, and adrenal insufficiency. early recognition and treatment of the infection, which are unclear, are vital to patient welfare. we studied the disease epidemiology and reviewed the ... | 2007 | 17674061 |
| the evolution of genetic structure in the marine pathogen, vibrio vulnificus. | multi-locus sequence types (mlst) from a global collection of vibrio vulnificus isolates were analysed for the contribution of recombination to the evolution of two divergent clusters of strains and a human-pathogenic hybrid genotype, which caused a disease outbreak in israel. recombination contributes more substantially than mutation to generating strain diversity. for allelic diversity within loci, the ratio of recombination to mutation events is approximately 2:1. the role of recombination re ... | 2007 | 17716955 |
| comparison of direct genome restriction enzyme analysis and pulsed-field gel electrophoresis for typing of vibrio vulnificus and their correspondence with multilocus sequence typing data. | we compared the potential of direct genome restriction enzyme analysis (dgrea) and pulsed-field gel electrophoresis (pfge) for discriminating vibrio vulnificus isolates from clinical (23) and environmental (17) sources. the genotypes generated by both methodologies were compared to previous multilocus sequence typing (mlst) data. dgrea established clearer relationships among v. vulnificus strains and was more consistent with mlst than with pfge. dgrea is a very promising tool for epidemiological ... | 2007 | 17720834 |
| identification of a new hemolysin from diarrheal isolate ssu of aeromonas hydrophila. | a clinical strain ssu of aeromonas hydrophila produces a potent cytotoxic enterotoxin (act) with cytotoxic, enterotoxic, and hemolytic activities. a new gene, which encoded a hemolysin of 439-amino acid residues with a molecular mass of 49 kda, was identified. this hemolysin (hlya) was detected based on the observation that the act gene minus mutant of a. hydrophila ssu still had residual hemolytic activity. the new hemolysin gene (hlya) was cloned, sequenced, and overexpressed in escherichia co ... | 2007 | 17725618 |
| procaspase-3 activation by a metalloprotease secreted from vibrio vulnificus. | vibrio vulnificus is a marine bacterium and a human pathogen capable of causing wound infection and septicemia. we previously showed that the metalloprotease vep secreted by v. vulnificus activates prothrombin in vitro. to further investigate the ability of vep to activate other zymogens, we used a mutant form of procaspase-3 which lacks the native cleavage sites as a zymogen. the mutant zymogen was activated by vep to yield a mature enzyme with a maximum increase in caspase-3 activity of approx ... | 2007 | 17786292 |
| antimicrobial susceptibilities of vibrio parahaemolyticus and vibrio vulnificus isolates from louisiana gulf and retail raw oysters. | the antimicrobial susceptibilities of 168 vibrio parahaemolyticus and 151 vibrio vulnificus isolates recovered from 82 louisiana gulf and retail oysters in 2005 and 2006 were determined. overall, the two vibrios remained susceptible to the majority of antimicrobials tested; reduced susceptibility was detected only in v. parahaemolyticus for ampicillin (81%; mic > or = 16 microg/ml). additionally, v. parahaemolyticus displayed significantly higher mics for cefotaxime, ciprofloxacin, and tetracycl ... | 2007 | 17827331 |
| phosphate and carbon source regulation of alkaline phosphatase and phospholipase in vibrio vulnificus. | in this study, the effects of phosphate concentration and carbon source on the patterns of alkaline phosphatase (apase) and phospholipase (plase) expression in vibrio vulnificus atcc 29307 were assessed under various conditions. the activities of these enzymes were repressed by excess phosphate (4 mm) in the culture medium, but this repression was reversed upon the onset of phosphate starvation in low phosphate defined medium (lpdm) containing 0.2 mm of phosphate at approximately the end of the ... | 2007 | 17846584 |
| vibrio vulnificus infection: diagnosis and treatment. | vibrio vulnificus infection is the leading cause of death related to seafood consumption in the united states. this virulent, gram-negative bacterium causes two distinct syndromes. the first is an overwhelming primary septicemia caused by consuming raw or undercooked seafood, particularly raw oysters. the second is a necrotizing wound infection acquired when an open wound is exposed to warm seawater with high concentrations of v. vulnificus. most patients, including those with primary infection, ... | 2007 | 17853628 |
| information from your family doctor. vibrio vulnificus infection: what you should know. | 2007 | 17853629 | |
| typing of vibrio vulnificus strains by variability in their 16s-23s rrna intergenic spacer regions. | amplification of the 16s-23s rdna spacer region (isr1) is a simple and rapid procedure for subtyping bacteria, especially those with several ribosomal operons including vibrio vulnificus. v. vulnificus contains nine ribosomal operons with four or five isr1 classes that differ in size and sequence. in the present study, 47 v. vulnificus strains of both shellfish and clinical origin were subtyped by their isr1 patterns using "universal" primers, which target conserved sequences located in the 16s ... | 2007 | 17883316 |
| a novel multiplex pcr for the identification of vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus. | to establish a simple multiplex polymerase chain reaction (pcr) that will identify vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus. | 2007 | 17897378 |
| vibrio vulnificus and v. parahaemolyticus necrotising fasciitis in fishermen visiting an estuarine tropical northern australian location. | three cases of severe necrotising fasciitis due to vibrio vulnificus (two cases) and vibrio parahaemolyticus (one case, fatal), have occurred in caucasian tourists while fishing at a remote tropical northern australian estuarine area. infections were acquired over a 4-year period during the tourist fishing season (april to july 2000-2003), when water temperatures range from 23 to 30 degrees c. they are notable for their geographical clustering in the remote western aspect of the gulf of carpenta ... | 2007 | 16890991 |
| climate change and the emergence of vibrio vulnificus disease in israel. | in 1996, a major unexplained outbreak of systemic vibrio vulnificus infection erupted among israeli fish market workers. the origins of this emergent infectious disease have not been fully understood. a possible link between climate change and disease emergence is being investigated. meteorological service data from 1981, the earliest detection and reporting of v. vulnificus for the time in israel, to 1998 for two stations located within the main inland fish farm industry were analyzed. the 1996 ... | 2007 | 16949069 |
| purification and characterization of a lectin from the banana shrimp fenneropenaeus merguiensis hemolymph. | a lectin from the hemolymph of the banana shrimp fenneropenaeus merguiensis was purified by affinity chromatography on a fetuin-agarose column following by gel filtration on a superose-12 column. the native molecular mass of purified f. merguiensis lectin (fml) determined by gel filtration was 316.2 kda and its carbohydrate content was estimated to be 4.4%. by sds-page analysis, purified fml consisted of 32.3 kda and 30.9 kda subunits. these data suggest that this lectin is an oligomer. two-dime ... | 2007 | 16934939 |
| isolation and characterization of a cold-induced nonculturable suppression mutant of vibrio vulnificus. | the viable but nonculturable (vbnc) suppression mutant formed platable cells at low temperature stress after inoculation in artificial seawater (asw). suppression subtractive hybridization was used to identify differentially expressed genes among cdnas of the vbnc suppression mutant and the wild-type vibrio vulnificus strain. glutathione s-transferase was identified as a responsive gene of the vbnc suppression mutant in our assay, and was highly expressed from the vbnc suppression mutant at low ... | 2007 | 16520028 |
| determination of the affinity of a steroid for its receptor is not sufficient to measure its intrinsic hormonal activity. | 2007 | 2219201 | |
| determination of the affinity of a steroid for its receptor is not sufficient to measure its intrinsic hormonal activity. | 2007 | 2219201 | |
| [methods of determining clinically sufficient decurarization and its evaluation]. | mechanomyoscopy as an objective method for determining the degree of decurarisation is particularly useful in the case of uncooperative patients and those who are incapable of adequate neck flexion mostly for spinal column diseases. the authors use for that purpose a czechoslovak-made neuromuscular stimulator (lsn 110), with constant 50 hz frequency of stimulation, 0.3 ms impulse duration, max. 50 ma and, depending on skin impedance, 5-140 v. the stimulator switch is manually controlled. the abs ... | 2007 | 2168290 |
| zambia: focus on humanitarian care of aids patients. | 2007 | 2045228 | |
| lead use in california industry: its prevalence and health implications. | an estimated 230,000 workers were reported to work in lead-using activities as a result of a 1986 statistical survey of employers in california. however, 68% of these workers worked in activities of relatively low hazard. this included 114,000 workers engaged in electronic soldering. over 1800 workers were reported to work in battery manufacture, a relatively high-hazard activity, but with consistent monitoring and protection. in contrast, over 4300 workers were involved in radiator repair activ ... | 2007 | 1951050 |
| pain, prostaglandins, and food lipids. influences of dietary polyunsaturated fatty acids on eicosanoid synthesis and effects. | 2007 | 2870709 | |
| [atypical and combined forms of infectious-allergic polyneuropathies]. | 2007 | 1865642 | |
| [avoid errors. preparation of abutments for removable denture cast framework]. | 2007 | 2151674 | |
| lauryl sulphate haemoglobin: a non-hazardous substitute for hicn in haemoglobinometry. | the haemiglobincyanide (hicn) method for measuring haemoglobin is used extensively worldwide; its advantage is the ready availability of a stable and internationally accepted reference standard/calibrator. however, its use may create a problem, especially in automated analysers, as the waste disposal of large volumes of reagent containing cyanide may constitute a potential toxic hazard. as an alternative, conversion of haemoglobin to a sulphate derivative by nontoxic sodium lauryl sulphate has b ... | 2007 | 1794230 |
| inbreeding in saguenay-lac-st-jean (quebec, canada): a study of catholic church dispensations 1842-1971. | saguenay-lac-st-jean (slsj) is a rather geographically isolated region of quebec which shows a high occurrence of hereditary disorders. it has been suggested that high inbreeding might explain this situation. we studied the inbreeding in the slsj region by 10-year periods from 1842 till 1971 using the catholic church dispensations. the values of the mean inbreeding coefficient were found to be low during the whole period, reaching a peak of 22.94 x 10(-4) during the period 1902-1911. the values ... | 2007 | 1797631 |
| observation and theory in psychoanalysis: the self psychology of heinz kohut. | the development of self psychology by heinz kohut illustrates the influence of observational method on theory formation in psychoanalysis. in 1959 kohut began to emphasize empathy and introspection over the traditional combination of free association and evenly suspended attention. this became an important part of a process of revision in psychoanalytic theory which culminated in self psychology. this paper demonstrates how and why that particular change in observational method influenced that p ... | 2007 | 1924603 |
| hybridocytochemistry with non-radioactive probes as a tool for biomedical research and cytodiagnostics. | the advent of dna and rna labelling techniques which allow non-autoradiographic detection of specific nucleic acid sequences has opened new possibilities for rapid diagnosis at the nucleic acid level. there are already practical applications. however, in order to fully compete with radioactive procedures an increase in sensitivity is still desired. | 2007 | 2435580 |
| transient hypercalcemia following overdoses of soluble aspirin tablets. | 2007 | 3864434 | |
| transient hypercalcemia following overdoses of soluble aspirin tablets. | 2007 | 3864434 | |
| coronary reserve in patients with aortic valve disease before and after successful aortic valve replacement. | in patients with aortic valve disease and normal coronary angiograms coronary reserve was determined by the coronary sinus thermodilution technique. three groups of patients were studied: 37 preoperative patients; 18 different patients 12-52 months after aortic valve replacement and seven control subjects with no cardiac disease. coronary flow ratio (dipyridamole/rest) was diminished in preoperative compared with postoperative patients (1.66 +/- 0.44 vs 2.22 +/- 0.85; p less than 0.05) as well a ... | 2007 | 2044545 |
| illumination and errors in dispensing. | the relationship between the level of illumination and the prescription-dispensing error rate in a high-volume army outpatient pharmacy was investigated. the prescription error rate was determined by direct, undisguised observation and retrospective prescription review under three levels of illumination (45, 102, and 146 foot-candles) during 21 consecutive weekdays. illumination was controlled in the prescription-checking area of the pharmacy by using additional fluorescent lamps and filters. th ... | 2007 | 1781468 |
| [psychological approach in nursing. delusion associated with alcoholism]. | 2007 | 2632860 | |
| [early and long-term results of percutaneous transluminal coronary angioplasty in angina pectoris]. | 2007 | 2258666 | |
| [early and long-term results of percutaneous transluminal coronary angioplasty in angina pectoris]. | 2007 | 2258666 | |
| [growth and therapy in angle class ii/2--a longitudinal cephalometric study]. | the influence of both growth and functional therapy with the u-bow-activator type i on angle class ii subdivision 2 malocclusion is analysed in a cephalometric long term study. findings concerning skeletal problems: 1. therapeutically intended clockwise rotation of the mandible with vertically and sagittally controlled maxillary position, 2. achieving normal sagittal mandibular growth rates. dental problems are improved significantly. the results obtained by functional therapy and growth proved ... | 2007 | 2227736 |
| [growth and therapy in angle class ii/2--a longitudinal cephalometric study]. | the influence of both growth and functional therapy with the u-bow-activator type i on angle class ii subdivision 2 malocclusion is analysed in a cephalometric long term study. findings concerning skeletal problems: 1. therapeutically intended clockwise rotation of the mandible with vertically and sagittally controlled maxillary position, 2. achieving normal sagittal mandibular growth rates. dental problems are improved significantly. the results obtained by functional therapy and growth proved ... | 2007 | 2227736 |
| [the importance of the compensatory powers of the lungs in determining the indications for surgical treatment of hypoplasia of the lungs]. | the author studied the compensatory possibilities of pulmonary respiration, gas exchange and hemodynamics of pulmonary circulation to determine the indications for operative treatment of simple hypoplasia of the lungs. the work is based on the results of examination of 58 patients. forty-five patients underwent an operation. it was established that simple hypoplasia of the lungs is characterized by mild ventilation disorders and deep disorders of gas exchange leading to manifest and latent hypox ... | 2007 | 2223215 |
| [the importance of the compensatory powers of the lungs in determining the indications for surgical treatment of hypoplasia of the lungs]. | the author studied the compensatory possibilities of pulmonary respiration, gas exchange and hemodynamics of pulmonary circulation to determine the indications for operative treatment of simple hypoplasia of the lungs. the work is based on the results of examination of 58 patients. forty-five patients underwent an operation. it was established that simple hypoplasia of the lungs is characterized by mild ventilation disorders and deep disorders of gas exchange leading to manifest and latent hypox ... | 2007 | 2223215 |
| [lung cancer in women in moscow (epidemiology, etiology and surgical treatment)]. | in the studied period (1973-1987) the lung carcinoma morbidity rate among females of moscow, in general intensive indices (per 100,000 females), showed a tendency to reduce (18.4 in 1973 and 15.1 in 1987). a reverse tendency is mostly encountered in other countries. more than half of patients with lung carcinoma were over 70 years of age. the ratio of males with lung carcinoma to that of females grew from 3.0:1.0 in 1973-1977 to 3.6:1.0 in 1983-1987. in the period from 1979 to 1988, 380 females ... | 2007 | 2223213 |
| [lung cancer in women in moscow (epidemiology, etiology and surgical treatment)]. | in the studied period (1973-1987) the lung carcinoma morbidity rate among females of moscow, in general intensive indices (per 100,000 females), showed a tendency to reduce (18.4 in 1973 and 15.1 in 1987). a reverse tendency is mostly encountered in other countries. more than half of patients with lung carcinoma were over 70 years of age. the ratio of males with lung carcinoma to that of females grew from 3.0:1.0 in 1973-1977 to 3.6:1.0 in 1983-1987. in the period from 1979 to 1988, 380 females ... | 2007 | 2223213 |
| no time for an aids backlash. | 2007 | 2045284 | |
| ovarian cancer. | 2007 | 2042058 | |
| [long-term results of the treatment of ruptured intracranial aneurysms. study of a series of 328 patients hospitalized from 1972 to 1984]. | between 1972 to 1984, 328 patients were admitted in the department of neurosurgery, for the treatment of a ruptured intracranial aneurysm, and being clinically in grades i to iv. these patients were submitted to a deferred surgery protocol. 5.5% of the patients died before surgery, and 94.5% were actually operated on. the follow-up was at least over one year, and up to 14 years (mean 3 years and 8 months). the long term result was appreciated according to the physical status of the patients and ... | 2007 | 3374722 |
| effect of oil-contaminated sediment on the longhorn sculpin (myoxocephalus octodecemspinosus) following chronic exposure. | 2007 | 1932866 | |
| [transient polyuria in pregnancy in diabetes insipidus and gestational diabetes]. | two pregnant women developed overt polyuria (up to 11 l/day) and polydipsia during their second and third trimesters of pregnancy. in one patient hydronephrosis was present. both patients suffered from mild gestational diabetes mellitus. plasma sodium was 145 and 162 mmol/l. polyuria and urinary hypo-osmolality responded well to desmopressin acetate. after delivery, polyuria and polydipsia disappeared in one patient and significantly improved in the other. infusion of hypertonic saline one and t ... | 2007 | 1770904 |
| failure of oocyte retrieval during in vitro fertilization: a sporadic event rather than a syndrome. | failure to retrieve oocytes during in vitro fertilization (ivf) treatment was recently referred to as the "empty follicle syndrome." data on the possible repetitiveness of this during previous or subsequent ivf attempts is lacking, which limits the understanding of its significance to the involved patients. of 26 patients who had cycles during which no oocytes were retrieved, 10 were pregnant in the past and 20 had at least one other ivf cycle that yielded oocytes. there was no difference in the ... | 2007 | 1899395 |
| cholestatic jaundice associated with captopril therapy. | captopril has attained widespread use as an effective agent in the treatment of heart failure and hypertension. dermatological, renal and haematological toxicity associated with its use has been widely described and is usually well recognized. there have been comparatively few reports implicating it as causing hepatic drug reactions. most descriptions have emphasized strongly cholestatic features, although a mixed hepatocellular cholestatic picture and predominant hepatocellular reactions have b ... | 2007 | 1932674 |
| familial predisposition for herniation of a lumbar disc in patients who are less than twenty-one years old. | the parents of sixty-three patients who were less than twenty-one years old and who had operatively confirmed herniation of a lumbar disc were interviewed regarding a history of sever back pain, sciatica, and herniated disc, to determine whether aggregation of herniation of a lumbar disc occurs in families of patients in this young age-group. the parents of sixty-three additional patients who had a non-spinal orthopaedic diagnosis (control group) were matched for age and sex with the study group ... | 2007 | 1824705 |
| fluorosis, osteopetrosis, and ectopic calcification. | 2007 | 2223455 |