Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| use of an oligonucleotide probe to detect vibrio parahaemolyticus in artificially contaminated oysters. | a 26-mer oligonucleotide specific to vibrio parahaemolyticus was synthesized from a 1,275-bp thermostable direct hemolysin (tdh) gene. this oligonucleotide probe specifically reacted with dna from 89 of 95 v. parahaemolyticus isolates but not with dna from other vibrios or other enteric and nonenteric organisms (n = 48). the probe hybridized with southern blots of 0.5-kb hindiii-restricted chromosomal dna fragments from all but five v. parahaemolyticus test isolates. the probe could be used to d ... | 1992 | 1444377 |
| [the comparative characteristics of vibrio cholerae hemolysins]. | biochemical and biological properties of in vivo and in vitro hemolysins of cholera germs isolated by different authors as well as hemolysins of vibrio parahaemolyticus and escherichia coli have been comparatively characterized. according to the above data hemolysin of cholera germ el tor is a thermolabile protein with molecular mass of 60-80 thou.; it is cytotoxic, enteropathogenic and lyses all the species of erythrocytes. hemolysins of v. eltor and germs are immunologically related. hemolysin ... | 1992 | 1453988 |
| identification of amino acid changes affecting yeast uroporphyrinogen decarboxylase activity by sequence analysis of hem12 mutant alleles. | the molecular basis of the uroporphyrinogen decarboxylase defect in eleven yeast 'uroporphyric' mutants was investigated. uroporphyrinogen decarboxylase, an enzyme of the haem-biosynthetic pathway, catalyses the decarboxylation of uroporphyrinogen to coproporphyrinogen and is encoded by the hem12 gene in the yeast saccharomyces cerevisiae. the mutations were identified by sequencing the mutant hem12 alleles amplified in vitro from genomic dna extracted from the mutant strains. four mutations lea ... | 1992 | 1471989 |
| transforming growth factor beta and basic fibroblast growth factor synergistically promote early bovine embryo development during the fourth cell cycle. | developmentally competent bovine blastocysts were produced by adding transforming growth factor beta (tgf beta) and basic fibroblast growth factor (bfgf) to serum-free cultures of in vitro produced, 2-cell bovine embryos. the effects of tgf beta were evaluated because this growth factor signals synthesis and secretion of the extracellular matrix component fibronectin and its receptor. previous investigations have demonstrated that fibronectin promotes early bovine embryo development in vitro. th ... | 1992 | 1472373 |
| purification and characterization of kanagawa haemolysin from vibrio parahaemolyticus. | the haemolysin of a kanagawa-phenomenon-positive vibrio parahaemolyticus strain was purified to apparent homogeneity by acid precipitation, deae-trisacryl, hydroxyapatite and fplc (mono-q) columns: 1.4 micrograms of protein gave a single band on conventional sds-page with silver staining. the haemolysin was not inactivated by heating for 10 min at 100 degrees c. it was a monomeric protein with a molecular weight estimated to be 29 kda by page under denaturing and non-denaturing conditions. the h ... | 1992 | 1475518 |
| the thermostable direct hemolysin of vibrio parahaemolyticus is a pore-forming toxin. | the hemolytic mechanism of thermostable direct hemolysin (tdh), a possible virulence factor of vibrio parahaemolyticus, was studied. we demonstrated that tdh acts as a "pore-forming toxin" in temperature-dependent and -independent steps. the first temperature-dependent step requires only about 1-2 min incubation at 37 degrees c and makes a "pore" with a functional diameter of approximately 2 nm. the pore size was deduced from the molecular diameter of the colloidal inhibitory polysaccharides. th ... | 1992 | 1477791 |
| ultrastructural alterations of brain cortex in rat following intraperitoneal administration of mercuric chloride. | intraperitoneal administration of a single dose (6 mg/kg body weight) of mercuric chloride (hgcl2) results in ultrastructural changes in brain cortex of the rats. 18 hours after administration of hgcl2 the accumulation of dense deposits of mercury in nerve and glial cell cytoplasm was observed. the quantity of microglia in neuropil was also increased in this experimental group. we postulate that these cerebral macrophages can play an important role in the process of intoxication, too. 5 days aft ... | 1992 | 1479187 |
| detection of the thermostable direct hemolysin gene (tdh) and the thermostable direct hemolysin-related hemolysin gene (trh) of vibrio parahaemolyticus by polymerase chain reaction. | polymerase chain reaction (pcr) protocols were established for specific detection of the tdh and trh genes, the virulence marker genes of vibrio parahaemolyticus encoding two related hemolysins. the tdh and trh genes are known to have sequence divergence of up to 3.3% and 16%, respectively. attempts were made to find suitable primer pairs and annealing temperatures to detect each gene without fail. dnas extracted from 36 representative strains of v. parahaemolyticus were used in the initial scre ... | 1992 | 1480187 |
| non-isotopic microtitre plate-based assay for detecting products of polymerase chain reaction amplification: application to detection of the tdh gene of vibrio parahaemolyticus. | a non-isotopic microtitre plate-based assay method was devised for detection of products of the polymerase chain reaction. this assay involves affinity immobilization of the biotinylated amplification products in microtitre plate wells and their fluorescence detection by their hybridization with an oligonucleotide probe linked to alkaline phosphatase. an advantage of this procedure is that the immobilization and hybridization are carried out simultaneously in the wells, thus shortening the assay ... | 1992 | 1480188 |
| [the phages of halophilic vibrios and their use]. | the range of the lytic activity of 46 phages of parahemolytic vibrios isolated from lysogenic strains, sea water samples, crabs and mussels has been studied. the phages are represented by virions belonging to morphological groups ii, iv, v according to the phage classification currently used in russia and to different serological groups. no relationship between the sensitivity of vibrio strains to the phages under study and the specificity of serotypes o and k has been established. the preparati ... | 1992 | 1481605 |
| professional doc (pdoc): a software environment for authoring and consulting of electronic documentation. interactive diagnosis and therapy: a medical application. | professional doc (pdoc) is a software environment for electronic documentation authoring and consulting based on the hypermedia technology. it allows to manage different kinds of information (pictures, sounds, texts, graphics, video) organized according to hyperstructures. the user-friendliness and effectiveness of its man-machine interface, and its availability on the most common hardware platforms makes it a suitable product for a very broad category of users. interactive diagnosis and therapy ... | 1992 | 1483002 |
| [the suicide phenomenon and fermentative metabolic activity in strains of the aeromonas group isolated from feces]. | we study the "suicide" phenomena as well as metabolic pathways of mixed acids (methyl red test, mr) and butylene glycol (voges-proskauer, vp), in 107 strains belonging to mesophilic aeromonas group, isolated from stools. the strains have been identified as a. hydrophila, 28 cases (26.1%), a. sobria 26 cases (24.3%) and a. caviae 53 cases (49.6%). all a. caviae strains behave homogeneously as f+, rm+ and vp-, independently of temperature (30 or 37 degrees c). a. hydrophila strains only showed the ... | 1992 | 1489794 |
| susceptibility of the brine shrimp artemia and its pathogen vibrio parahaemolyticus to chlorine dioxide in contaminated sea-water. | adults and nauplii of the brine shrimp, artemia, together with vibrio parahaemolyticus, were placed in sewage-contaminated sea-water which had been treated with chlorine dioxide (hallox e-100tm) to test its potential as a disinfectant for salt water aquaculture. the nauplii were very susceptible to low concentrations of chlorine dioxide (47 micrograms/l cl-), but the adults were slightly more resistant. sterile sea-water treated with lower concentrations of chlorine dioxide (less than 47 microgr ... | 1992 | 1490907 |
| enterotoxigenicity of vibrio parahaemolyticus with and without genes encoding thermostable direct hemolysin. | vibrio parahaemolyticus produces a thermostable direct hemolysin (tdh) that has been implicated in the pathogenesis of diarrheal disease caused by this organism. however, previous studies attempting to demonstrate the contribution of the hemolysin to virulence have been inconclusive. we investigated this putative virulence factor by using an isogenic tdh-negative (tdh-) strain constructed by specifically inactivating the two copies of the tdh gene encoding tdh. the enterotoxigenicities of the pa ... | 1992 | 1500161 |
| behavioral analysis of vibrio parahaemolyticus variants in high- and low-viscosity microenvironments by use of digital image processing. | digital image analysis and light microscopy were used to study and quantify the growth and behavior of two variants and selected flagellar mutants of vibrio parahaemolyticus in glass flow cells under high- and low-viscosity conditions. the observations showed a series of surface-associated behaviors, including attachment, microcolony formation, migration, chemotactic movements, and aggregation, indicating a substantial degree of adaptive flexibility and multicellular behavior during growth of v. ... | 1992 | 1512206 |
| sequence variation in the thermostable direct hemolysin-related hemolysin (trh) gene of vibrio parahaemolyticus. | our previous molecular epidemiologic study with gene probes (h. shirai, h. ito, t. hirayama, y. nakamoto, n. nakabayashi, k. kumagai, y. takeda, and m. nishibuchi, infect. immun. 58:3568-3573, 1990) demonstrated that the gene (trh) encoding a thermostable direct hemolysin-related hemolysin was strongly associated with clinical strains of vibrio parahaemolyticus. strain-to-strain variation in the intensities of the hybridization signals observed in the above study also suggested that the trh gene ... | 1992 | 1514791 |
| development of the immunomagnetic enrichment method selective for vibrio parahaemolyticus serotype k and its application to food poisoning study. | a method using immunomagnetic separation was developed to isolate the specified k serotype of vibrio parahaemolyticus from a mixture of a large number of bacteria with other k serotypes. this method was applied to food poisoning studies and could recover the v. parahaemolyticus serotype found in the patient from the incriminated foods. | 1992 | 1514817 |
| [sensitivity of vibrio and aeromonas to antibiotics]. | the antibiotic sensitivity of 696 cultures belonging to the family vibrionaceae (v. cholerae o1, v. cholerae non-o1, v. albensis, v. parahaemolyticus, v. alginolyticus and aeromonas spp.) was studied and general regularities of the antibiotic sensitivity were shown: a high sensitivity to broad-spectrum antibiotics (tetracycline and chloramphenicol) and a low sensitivity to ++beta lactams (carbenicillin and ampicillin). the comparative examinations revealed similarity in the antibioticograms of v ... | 1992 | 1514858 |
| evidence for the existence of a restriction-modification system common to several species of the family vibrionaceae. | a broad-host-range vibriophage kvp40 originally isolated on vibrio parahaemolyticus 1010 was restricted and modified by strains of at least five vibrio and one photobacterium species. 1010 was a non-restricting host. an anti-restriction mutant kvp40 aar1 was isolated after propagating the phage on a restricting host, v. anguillarum vib36. kvp40 aar1 grown on either 1010 or vib36, as well as the parental phage grown on vib36, showed much higher efficiencies of plating on all the restricting hosts ... | 1992 | 1521769 |
| effects of iron limitation on production of a siderophore, outer membrane proteins, and hemolysin and on hydrophobicity, cell adherence, and lethality for mice of vibrio parahaemolyticus. | vibrio parahaemolyticus is one of the most important enteropathogens in taiwan, japan, and other coastal regions. the pathogenesis of v. parahaemolyticus disease is not clearly understood. the expression of some factors by v. parahaemolyticus in iron-rich and iron-limited media was analyzed. in the clinical hemolytic strains, the production of a siderophore, two outer membrane proteins (77 and 80 kda), and thermostable direct hemolysin was significantly enhanced in iron-limited culture, and hemo ... | 1992 | 1535342 |
| risk factors for suicide attempts in a human immunodeficiency virus screening program. | 1992 | 1539099 | |
| cloning and expression of a cytosolic megakaryocyte protein-tyrosine-phosphatase with sequence homology to retinaldehyde-binding protein and yeast sec14p. | protein tyrosine phosphorylation is important in the regulation of cell growth, the cell cycle, and malignant transformation. we have cloned a cdna that encodes a cytosolic protein-tyrosine-phosphatase (ptpase), meg2, from meg-01 cell and human umbilical vein endothelial cell cdna libraries. the 4-kilobase cdna sequence of ptpase meg2 corresponds in length to the mrna transcript detected by northern blotting. the predicted open reading frame encodes a 68-kda protein composed of 593 amino acids a ... | 1992 | 1557404 |
| detection and quantitative assessment of a vibrio cholerae o1 species in several foods by a novel enzyme immunoassay. | a selected antibody enzyme immunoassay (saeia) for the general detection of vibrio cholerae o1 species has been developed using the immunological reagents of a rabbit antiserum specific for v. cholerae o1 classical inaba 569b and immobilized cell fragments of v. cholerae o1 el tor 85p6, and beta-d-galactosidase-labeled goat anti-rabbit immunoglobulin g as tracer. the saeia was specific for v. cholerae o1 species and showed low cross-reaction values to other microorganism species tested including ... | 1992 | 1584068 |
| a broad-host-range vibriophage, kvp40, isolated from sea water. | a broad-host-range vibriophage, kvp40, was isolated from sea water by using vibrio parahaemolyticus 1010 (eb101) as the indicator host. the host range of kvp40 extended over at least 8 vibrio and 1 photobacterium species. kvp40 was a large tailed phage containing double-stranded dna and belonged to ackermann's morphotype a2. kvp40 dna was cleaved by 11 different type ii restriction endonucleases including ecori and hindiii, but not by 17 other enzymes including bamhi, kpni and sali. | 1992 | 1584076 |
| the highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes. | chromosome in situ suppression hybridization has been carried out on human metaphase chromosomes to localize the g+c-richest human dna fraction (which only represents 3.5% of the genome), as isolated by preparative equilibrium centrifugation in cs2so4/3,6-bis(acetatomercurimethyl)-1,4-dioxane density gradient. this fraction essentially corresponds to isochore family h3. the rationale for carrying out this experiment is that this isochore family has, by far, the highest gene concentration, the hi ... | 1992 | 1594593 |
| haemagglutinating and buccal epithelial cell adherence activities of vibrio parahaemolyticus: correlation with virulence. | clinical and environmental isolates of vibrio parahaemolyticus were examined for their ability to agglutinate human and rabbit erythrocyte and to adhere to human buccal epithelial (hbe) cells in the presence or absence of mannose. all strains produced cell-associated haemagglutinins (hgs) after 3 h at 37 degrees c. mannose-sensitive haemagglutination (msha) appeared to be a significant marker for differentiating between clinical and environmental isolates; 75% of clinical and 11% of environmenta ... | 1992 | 1597877 |
| comparison of vibrio parahaemolyticus hemolysin (vp-trh) produced by environmental and clinical isolates. | tdh-related hemolysin (vp-trh) produced by kanagawa-phenomenon-negative (kp-) vibrio parahaemolyticus has been demonstrated to be a possible virulence determinant. though almost half of kp- isolates examined from diarrhoeal patients produced vp-trh, few reports mentioned the ability of environmental isolates to produce vp-trh. considering the route of infection with v. parahaemolyticus, this toxin must be produced by the organisms in the sea or in sea food. to confirm that vp-trh produced by v. ... | 1992 | 1601286 |
| chemotactic activity of hemocytes derived from two marine neritid gastropod molluscs, nerita albicilla and heminerita japonica, to vibrio parahaemolyticus and escherichia coli strains. | hemocytes of two marine neritid gastropods, nerita albicilla and heminerita japonica, were attracted chemotactically to live vibrio parahaemolyticus and escherichia coli strains. chemotactic attraction of n. albicilla hemocytes was enhanced in the presence of n. albicilla plasma, while that of h. japonica hemocytes was not enhanced in the presence of h. japonica plasma. chemotactic activity of the hemocytes seems to participate in the rapid elimination of v. parahaemolyticus from these gastropod ... | 1992 | 1606253 |
| examination by site-directed mutagenesis of the amino acid residues of the thermostable direct hemolysin of vibrio parahaemolyticus required for its hemolytic activity. | the amino acid sequences of eight variants of thermostable direct hemolysin (tdh) and a tdh-related hemolysin, deduced from nucleotide sequences, were compared. fourteen amino acid residues, mainly within conserved regions, were chosen to prepare mutant tdhs with one or two amino-acid replacements by site-directed mutagenesis. of the 25 mutant tdhs prepared, those with replacements of trp65 (trp65-his65, trp65-tyr65, trp65-leu65) or leu66 (leu66-ser66) showed very significant reduction (more tha ... | 1992 | 1630298 |
| low ph deforms the influenza virus envelope. | influenza virus membrane fusion is induced by low ph, which triggers an irreversible conformational change in the viral haemagglutinin (ha). the result of this change is the extrusion of the ha fusion peptide, after which it may act in the fusion of virus and endosomal membranes. here we describe electron microscopic observations on low ph-treated virus after negative staining or cryo-electron microscopy of virus in the frozen hydrated state. the results indicate a destabilization of the virus m ... | 1992 | 1634881 |
| [hairy cell leukemia successfully treated with 2'-deoxycoformycin following refractoriness to splenectomy and alpha-interferon therapy]. | a 34-year-old man was admitted in may, 1989 because of severe left upper abdominal pain, which was caused by advanced splenomegaly. on initial examinations, peripheral blood showed leukocytosis (13,400/ml) including 60% hairy cell which also infiltrated in bone marrow (64%). the patient was diagnosed as having hairy cell leukemia (japanese type) of b-cell lineage. splenectomy was performed as an initial treatment. the effect of splenectomy was only palliative and transient leukocytosis progresse ... | 1992 | 1635172 |
| total hepatic mesh wrap for hemostasis. | to improve hemostasis and avoid the complications of perihepatic packing, a technique of hepatic tamponade using total mesh wrap is reported. the method is geometrically, technically and mechanically feasible and appears to be effective in controlling severe parenchymatous bleeding. | 1992 | 1636144 |
| enzyme-labeled oligonucleotide probes for detection of the genes for thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) of vibrio parahaemolyticus. | alkaline phosphatase conjugated oligonucleotide probes were developed to detect the genes (tdh and trh) coding for the thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) of vibrio parahaemolyticus. using dot blot hybridization, probes were tested with 94 clinical isolates of v. parahaemolyticus. results agreed well with those obtained using radio-labeled recombinant dna probes for the genes tdh and trh. specificity and sensitivity of enzyme tdh probes for detection of the trh ge ... | 1992 | 1643584 |
| isolation and characterization of new restriction endonucleases from vibrio parahaemolyticus: vpak32i enzyme with the class-iis heptanucleotide specificity, gctcttcn1/n4. | six restriction endonucleases (enases), classified into four different specificities, were found in a screen among 68 reference strains of vibrio parahaemolyticus of human origin. five of these enases are isoschizomers of well-known enases, while the remaining one, designated vpak32i, is a novel and highly efficient class-iis enase with the hepatanucleotide recognition site, 5'-gctcttc(1/4)-3'. | 1992 | 1644299 |
| [experimental study of hyperostosis induced by hypervitaminosis a]. | radiological and histological studies were made on retinoid-induced hyperostosis in rats. vitamin a (va) was administered intraperitoneally in rats for 6 months. hyperostosis was observed in 94 percent of rats administered va and in 38 percent of the control. chondrocytes and vascular proliferation were observed in the attachment of the tendons and in the anterior corner of the vertebral body after 3 months. hyperostosis was observed as osteophytes in the attachment of ligaments or tendons and a ... | 1992 | 1280297 |
| isolated severe thrombocytopenia and bleeding caused by piperacillin. | bleeding and severe thrombocytopenia developed in a 71-year-old man who had been receiving piperacillin 5 g intravenously every 8 h for 9 days for the treatment of pseudomonas aeruginosa septicemia. after piperacillin was discontinued, the platelet counts became normal. rechallenge was made with 2 g of piperacillin intravenously resulting in thrombocytopenia within 6 h of piperacillin administration. the platelet count normalized after 3 days when no further piperacillin was given. | 1992 | 1287819 |
| [bacteriological study of traveller's diarrhoea. 4) isolation of enteropathogenic bacteria from patients with traveller's diarrhoea at osaka airport quarantine station during 1984-1991]. | during the last 8 years (1984 to 1991), 16,639,233 overseas travellers were quarantined at osaka airport quarantine station and 38,326 travellers reported that they were (or had been) suffering from diarrhoea. bacteriological examination of stools from 12,573 persons revealed the following results. 1) various enteropathogenic bacteria were isolated from 3,669 cases (29.2%) examined. the predominant species of bacteria isolated were as follows: salmonella, 1049 cases; plesiomonas shigelloides, 10 ... | 1992 | 1293222 |
| [the lysogeny of vibrio parahaemolyticus of serovar o4:k12]. | lysogeny has been first established in strains of parahemolytic vibrios of serovar o4:k12. moderate phages belonged to morphological group iv by home a. s. tikhonenko's classification and were presented by one serological type. no correlation has been revealed between sensitivity to moderate phages of parahemolytic vibrios and specificity of "o"- or "k"-serotypes. | 1992 | 1293486 |
| features of cholera and vibrio parahaemolyticus diarrhoea endemicity in calabar, nigeria. | the clinical and epidemiological features of acute vibrio diarrhoeal disease were studied in 881 patients seen at the university of calabar teaching hospital (ucth), calabar, nigeria, between january and december 1989. stools and rectal swabs of patients and randomly-selected control subjects were microscopically and culturally examined for the presence of enteric pathogens. households of vibrio diarrhoea cases and matched controls were visited for ecologic studies. of a total of 108 (12.3%) cul ... | 1992 | 1294392 |
| a survey of urease-positive vibrio parahaemolyticus strains isolated from traveller's diarrhea, sea water and imported frozen sea foods. | the frequency of urease-positive vibrio parahaemolyticus among isolates from patients, imported frozen sea foods and the environment (sea water) was studied. the highest isolation frequency of urease-positive v. parahaemolyticus was found in clinical isolates (11.2% out of 204 strains examined). urease-positive v. parahaemolyticus was found in 5.7% of 88 frozen sea food-isolates examined, but no strains isolated from sea water were urease-positive. the isolates were further examined for the prod ... | 1992 | 1294393 |
| purification and characterization of monomeric isocitrate dehydrogenase with nadp(+)-specificity from vibrio parahaemolyticus y-4. | nadp(+)-dependent isocitrate dehydrogenase [idh: ec 1.1.1.42] was purified to electrophoretic homogeneity from vibrio parahaemolyticus y-4, and shown to be a monomeric protein of molecular weight 80,000 with a pi of 5.0. the amino acid composition and partial sequence at the n-terminus resembled those reported for other bacterial monomeric idhs. immunotitration with antisera to the monomeric and dimeric enzymes (antisera to idh-ii and -i of vibrio abe-1) showed an immunochemical distinction betw ... | 1992 | 1295895 |
| effects of phosphate in the production of hemolysin in vibrio parahaemolyticus. | vibrio parahaemolyticus is known to produce several types of hemolysin, the most document is the tdh and trh. a new type of hemolysin whose expression is repressed by the presence of phosphate in the medium is reported. | 1992 | 1298639 |
| microbiological aspects and technological need: technological needs for nitrates and nitrites. | nitrate and nitrite are used in meat and fish curing, and in the manufacture of certain cheeses. nitrate itself has little antimicrobial effect and in most applications could be replaced by lower concentrations of nitrite. further, improved hygiene diminishes the need for nitrite. the antimicrobial activity and technological needs for nitrate and nitrite are reviewed. it is concluded that the technological needs for nitrite in meat products stored at < 10 degrees c could be met by added nitrite ... | 1992 | 1298641 |
| [vestibular evoked potentials. diagnostic trends]. | the contribution of short-latency evoked potentials to the exploration of auditory, visual and somatosensory neurosensory pathways is capital. the determination of short-latency vestibular evoked potentials has encountered many difficulties, mainly due to the necessity to find a specific, very short stimulation in order to select and synchronize the vestibular nerve fibers. recent studies in animals have demonstrated short-latency potentials evoked by angular accelerations, linear accelerations ... | 1992 | 1304102 |
| expression and release of neuroregulators during development: monoamines and neuropeptides. | the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ... | 1992 | 1306802 |
| brazilian urease-positive strains of vibrio parahaemolyticus carry genetic potential to produce the tdh-related hemolysin. | 1992 | 1308550 | |
| polar and lateral flagellar motors of marine vibrio are driven by different ion-motive forces. | various species of marine vibrio produce two distinct types of flagella, each adapted for a different type of motility. a single, sheathed polar flagellum is suited for swimming in liquid medium, and numerous unsheathed lateral flagella, which are produced only under viscous conditions, are suited for swarming over viscous surfaces. both types of flagella are driven by reversible motors embedded in the cytoplasmic membrane. here we report that the energy source for the polar flagellar motor of v ... | 1992 | 1309599 |
| kappa-opioid receptor stimulation abolishes mu- but not delta-mediated inhibitory control of spinal met-enkephalin release. | the possible opioid control through delta, mu and kappa receptors of the spinal release of met-enkephalin-like material (melm) was investigated in halothane-anaesthetized rats. the intrathecal perfusion of the delta agonist dtlet (10 microm) or the mu agonist dago (10 microm) resulted in a marked inhibition of melm release, which could be prevented by the selective antagonists naltrindole and naloxone, respectively. although the kappa agonist u 50488 h (10 microm) was inactive per se, it complet ... | 1992 | 1317026 |
| catabolite repression of the h(+)-translocating atpase in vibrio parahaemolyticus. | cells of vibrio parahaemolyticus grown in the presence of glucose showed reduced (by about 40%) oxidative phosphorylation. with this observation as a basis, we examined the effect of glucose on the level of h(+)-translocating atpase. the addition of glucose to the growth medium reduced the specific activity and the amount of the h(+)-translocating atpase in membrane vesicles of v. parahaemolyticus. these reductions were reversed by adding cyclic amp (camp) to the growth medium. we cloned some pa ... | 1992 | 1328162 |
| fimbriation, capsulation, and iron-scavenging systems of klebsiella strains associated with human urinary tract infection. | thirty-two strains of klebsiella pneumoniae and seven strains of klebsiella oxytoca isolated from urinary tract infections in elderly adults were analyzed for capsular antigens, iron-scavenging systems, and fimbriation. all strains were capsulated. twenty-seven different k antigens were identified among the strains, with no particular antigen dominating. all strains produced the iron-scavenging system enterochelin as analyzed by bioassay and dna hybridization. in contrast, the aerobactin iron-se ... | 1992 | 1347287 |
| [h2-receptor antagonist-refractory ulcer--its pathophysiology and role of proton-pump inhibitors]. | among h2-receptor antagonist (h2ra)-refractory ulcers, non-responders that did not heal after 5 months therapy had high intraluminal ph in the basal condition and high sensitivity to inhibition of acid secretion by h2ra but possessed gastric mucosa to generate less prostaglandins. combination therapy of pge1-analogue with h2ra healed these ulcers by 60%. proton-pump inhibitor (ppi) exerted a complete inhibition of acid secretion in these patients and the rate of healing was 88%. helicobacter pyl ... | 1992 | 1347325 |
| the role of pili in colonization of the rabbit intestine by vibrio parahaemolyticus na2. | vibrio parahaemolyticus na2 and its pili were studied in relation to intestinal colonization. the isolated pili were adhesive to the intestinal epithelium. the adhesion of the organisms was blocked by masking the epithelial receptor with the purified pili, or by treating the organisms with anti-pilus antibody (fab fraction). these results suggest that the pili of v. parahaemolyticus na2 play an important role in the adhesion of the organisms to the rabbit intestine. | 1992 | 1350048 |
| lomefloxacin versus trimethoprim/sulfamethoxazole in the treatment of adults with acute bacterial diarrhea. | this study compared the efficacy and safety of lomefloxacin with that of trimethoprim/sulfamethoxazole (tmp/smx) in the treatment of adult patients with acute diarrhea of bacterial etiology. two centers enrolled a total of 133 patients; 99 (74%) presented with severe symptoms. the patients were randomized to receive either lomefloxacin 400 mg once daily (n=68) or tmp/smx 160/800 mg twice daily (n=65) for five days. bacteriologic success was achieved in 89.5% of evaluation lomefloxacin-treated pa ... | 1992 | 18611521 |
| [hygienic and commercial quality of senegalese frozen shrimp]. | 100 samples of shrimps (frozen entire raw shrimps: fers and frozen peeled raw shrimps: fprs) collected in one urban factory, were studied to assess their hygienic and commercial quality. in addition to bacteriological analysis, the dosage of residual bisulphite content was carried out. it appeared that: level of aerobic plate was high for fers: 2.55.10(4)/g and weak for fprs: 2.45.10(5)/g; level of psychrotophic micro organisms was relatively high for fers: 1.07.10(4)/g and very weak for fprs: 0 ... | 1993 | 7882843 |
| lipopolysaccharide isolated from a new o-antigenic form (o13) of vibrio parahaemolyticus. | the chemical properties of a lipopolysaccharide (lps) isolated from a new o-antigenic form (o13) of vibrio parahaemolyticus were investigated. the lps contained glucose, galactose, l-glycero-d-manno-heptose and glucosamine. 2-keto-3-deoxy-octonate (kdo) was not detected in the lps by the periodate-thiobarbituric acid test (weissbach's reaction) under conventional hydrolysis conditions. instead, phosphorylated kdo (x1 and x2) was found in its strong-acid hydrolysate. this sugar composition was id ... | 1993 | 7684809 |
| a chemotaxonomic study of vibrio fluvialis based on the sugar composition of the polysaccharide portion of the lipopolysaccharides. | a chemotaxonomic study was carried out with a new serotyping scheme comprising 35 o-antigen groups of vibrio fluvialis on the basis of the sugar composition of the polysaccharide portion of their lipopolysaccharide (lps). a previously developed rapid method of preparing samples for compositional sugar analysis was employed. the 35 o-antigen groups were divided into 21 chemotypes. it is noted that a rarely occurring component sugar of gram-negative bacterial lps, d-glycero-d-manno-heptose, and tw ... | 1993 | 7684810 |
| [monitoring of halophilic vibrios in sea waters of the northern coasts of the province of rome]. | 1993 | 7524555 | |
| septicemia caused by vibrio parahemolyticus: a case report. | vibrio parahemolyticus is a halophilic marine vibrio commonly associated with outbreaks of acute gastroenteritis which also sometimes causes serious wound infection. it is an uncommon cause of septicemia. a few reports suggest that patients with chronic liver disease and leukemia are more susceptible. a case of liver cirrhosis with septicemia caused by this organism is discussed. the patient's condition rapidly deteriorated, and he died 12 hours after admission. | 1993 | 8299034 |
| inhibition of psychrotrophic organisms by propionicin plg-1, a bacteriocin produced by propionibacterium thoenii. | propionibacterium thoenii strain p127, which produces the bacteriocin propionicin plg-1, was grown in a skim milk medium and produced bacteriocin in that medium. no bacteriocin activity was detected in skim milk medium in which strain p127-1, a bacteriocin-negative variant of strain p127, had been grown. five psychrotrophic spoilage or pathogenic organisms (one strain each of listeria monocytogenes, pseudomonas fluorescens, vibrio parahaemolyticus, yersinia enterocolitica, and one strain of cory ... | 1993 | 8326023 |
| introduction of a series of alkyl thiomaltosides, useful new non-ionic detergents, to membrane biochemistry. | we synthesized a series of non-ionic detergents, alkyl thiomaltosides, and investigated their properties and usefulness. we solubilized membrane proteins of vibrio parahaemolyticus using the detergents. with octyl thiomaltoside, nonyl thiomaltoside, decyl thiomaltoside, or undecyl thiomaltoside, we observed satisfactory solubilization of the membrane proteins. alkyl thiomaltosides possessing longer alkyl chains showed better solubilization than ones possessing shorter chains. h(+)-translocating ... | 1993 | 8340350 |
| enterotoxigenic bacteria in the sudden infant death syndrome. | faecal samples from 123 infants who died with sudden infant death syndrome (sids) and from a comparative group of 52 age-matched babies were analysed for toxigenic bacteria and their toxins. serum samples from the sids infants were also analysed for these toxins. a significantly higher proportion of toxigenic bacteria and their toxins were found in faecal samples of sids babies than in samples from the comparative group. these toxins were also found in serum from the sids babies. clostridium per ... | 1993 | 8345506 |
| heat and acid sensitivity of motile aeromonas: a comparison with other food-poisoning bacteria. | the present study was undertaken to compare the heat and acid sensitivity of aeromonads with those of other food-poisoning bacteria. it became obvious that aeromonads were more sensitive to heat than escherichia coli o157:h7, staphylococcus aureus, and salmonella typhimurium. aeromonads were killed in peptone water within 2 min at 55 degrees c, while the other bacteria survived heating at 55 degrees c for more than 15 min. aeromonas cells were also less resistant to heat in hamburger steaks. the ... | 1993 | 8347426 |
| reactive arthritis induced by vibrio parahaemolyticus. | we describe a case of reactive arthritis after vibrio parahaemolyticus infection. v. parahaemolyticus is the principal bacteria involved in the causation of foodborne gastroenterocolitis outbreaks in japan. several bacteria have been recognized as being involved in the development of reactive arthritis and this is the first report of reactive arthritis induced by v. parahaemolyticus. | 1993 | 8350314 |
| identification of oligosaccharides consisting of d-glucuronic acid and l-glycero-d-manno- and d-glycero-d-manno-heptose isolated from vibrio parahaemolyticus o2 lipopolysaccharide. | 1993 | 8370031 | |
| [incidence of bacterial contamination in the ares-betanzos estuary (nw spain)]. | the presence of bacterial indicators of fecal pollution and v. parahaemolyticus in the estuary of ares-betanzos (ría de ares-betanzos, nw of spain) was investigated. resistance patterns of coliform bacteria to eight antibacterial agents were also determined. in general, high numbers of indicator bacteria were found; for instance, heterotrophic bacteria ranged between 1.82 x 10(2) to 1.9 x 10(4) cfu/ml and up to 4.6 x 10(3)/100 ml fecal coliforms in surface waters and 1.2 x 10(4)/100 ml fecal str ... | 1993 | 8397962 |
| cation flux studies of the lesion induced in human erythrocyte membranes by the thermostable direct hemolysin of vibrio parahaemolyticus. | vibrio parahaemolyticus, an important agent of seafood-borne gastroenteritis, expresses several putative virulence factors that could account for the disease symptoms of infected humans, namely, diarrhea, nausea, and abdominal cramps. the pathogenicity of v. parahaemolyticus correlates well with the kanagawa phenomenon (the hemolytic ability of strains grown on wagatsuma blood agar), implicating the thermostable direct hemolysin (tdh) as the predominant toxin responsible for pathogenicity. tdh-i ... | 1993 | 8406820 |
| treatment of acute bacterial diarrhea: a multicenter international trial comparing placebo with fleroxacin given as a single dose or once daily for 3 days. | this study was designed to test the efficacy of 400 mg fleroxacin given orally as a single dose or once daily for 3 days against acute bacterial diarrhea. a group of 508 adults with acute diarrhea were entered into a randomized, double-blind, placebo-controlled, multicenter trial. patients were examined and asked about numbers of liquid stools daily for 3 days and at 5 days after start of treatment. repeat stool samples were obtained for culture on days 3 and 5 after start of treatment. a total ... | 1993 | 8452179 |
| ciprofloxacin and loperamide in the treatment of bacillary dysentery. | to compare the safety and efficacy of loperamide plus ciprofloxacin with those of ciprofloxacin alone in the treatment of bacillary dysentery. | 1993 | 8452323 |
| identification of genes encoding components of the swarmer cell flagellar motor and propeller and a sigma factor controlling differentiation of vibrio parahaemolyticus. | vibrio parahaemolyticus possesses two distinct motility systems, the polar system used for swimming in liquid environments and the lateral system used for swarming over surfaces. growth on surfaces induces swarmer cell differentiation and expression of the lateral motility system. mutants, created by transposon mutagenesis of a clone expressing lateral flagellin and gene disruption in v. parahaemolyticus, were unable to swarm and failed to make lateral flagellin; therefore, unlike the case for t ... | 1993 | 8501040 |
| release of the outer membrane vesicles from vibrio cholerae and vibrio parahaemolyticus. | we found numerous small vesicles released from the cell by thin sectioning of the plate culture of vibrio cholerae and v. parahaemolyticus fixed with the freeze-substitution technique. from the broth media of exponentially growing bacteria we could collect the vesicles by the centrifugation but not enough without fixation. the vesicles are encompassed with a membrane structure similar to the outer membrane of these bacteria. the anti-o (inaba) serum reacted with the surface of the vesicles and t ... | 1993 | 8502178 |
| vibrio parahaemolyticus septicemia associated with water-skiing. | 1993 | 8507240 | |
| vibrio parahaemolyticus has a homolog of the vibrio cholerae toxrs operon that mediates environmentally induced regulation of the thermostable direct hemolysin gene. | in an effort to identify the regulatory gene controlling the expression of the tdh gene, encoding the thermostable direct hemolysin of vibrio parahaemolyticus, we examined total dna of aq3815 (a kanagawa phenomenon-positive strain) for sequences homologous to that of the toxr gene of vibrio cholerae. the extracted dna gave a weak hybridization signal under reduced-stringency conditions with a toxr-specific dna probe. cloning and sequence analysis of the probe-positive sequence revealed an operon ... | 1993 | 8509337 |
| luminol-dependent chemiluminescence of hemocytes derived from marine and estuarine molluscs. | hemocytes of two marine molluscs, nerita albicilla (gastropod) and mytilus edulis (bivalve), were stimulated in vitro with zymosan and live cells of vibrio parahaemolyticus and escherichia coli as determined by luminol-dependent chemiluminescence (cl). the cl response was enhanced in the presence of the respective molluscan plasma. hemocytes of an estuarine gastropod, clithon retropictus, showed low cl response to zymosan and v. parahaemolyticus, which was slightly enhanced in the presence of c. ... | 1993 | 8513011 |
| [incidence of vibrio parahaemolyticus in raw fish, oysters, and shrimp]. | the incidence of vibrio parahaemolyticus in fresh seafood sold in guadalajara, was studied by two procedures. these two procedures were compared to choose a reliable technique when outbreaks of v. parahaemolyticus illness occur. for one year, 57 samples of fresh oysters, fish and shrimp were analyzed for mesophilic aerobic bacteria (mab) content, v. parahaemolyticus and ph. total volatile nitrogen (tvn) was also determined in samples of fish and shrimp. mab were counted by the pour plate method, ... | 1993 | 8047729 |
| extraintestinal infections caused by vibrio parahaemolyticus and vibrio alginolyticus in a danish county, 1987-1992. | over a 6-year period we detected 30 clinical infections caused by halophilic vibrios in a restricted geographical area. vibrio parahaemolyticus infections were found in 13 patients (3 with wound infections, 10 with ear infections), and vibrio alginolyticus infections in 17 patients, all of whom had ear infections. from 1987 to 1990, infections caused by marine vibrios were found in at most 4 cases annually, in 1992 in 6 instances, whereas in a 5-month period in 1991 we experienced 15 cases of ex ... | 1993 | 8052813 |
| vibrios among patients of good socioeconomic conditions during the cholera epidemic in recife, brazil. | between march and july, 1992, we screened for vibrio all fecal samples submitted for bacteriologic diagnosis at a private clinical laboratory in recife. of 1435 cultures examined only 1 (0.07%) was positive for v. cholerae 01, biovar eltor, serovar inaba, but 17 (1.2%) yielded non-cholera vibrio (v. cholerae non-01; v. fluvialis; v. furnissii, v. parahaemolyticus and vibrio spp). thus, v. cholerae 01, differently of other enteropathogenic vibrios, spared individuals of good socioeconomic conditi ... | 1993 | 8115794 |
| rapid and specific detection of the thermostable direct haemolysin gene in vibrio parahaemolyticus by the polymerase chain reaction. | synthetic oligonucleotide primers derived from a sequence of the thermostable direct haemolysin (tdh) gene were used in a polymerase chain reaction (pcr) amplification technique to detect this gene in strains of vibrio parahaemolyticus. a total of 36 tdh-producing, and 89 tdh-negative vibrio parahaemolyticus strains and 46 other vibrios and enteric pathogens were studied. in all, 36 strains of vibrio parahaemolyticus from which the tdh gene could be successfully amplified by pcr were found to be ... | 1993 | 8126441 |
| evaluation of virulence factors in environmental isolates of vibrio species. | strains of vibrio parahaemolyticus, vibrio fluvialis and vibrio mimicus isolated from seafood and seawater were examined for characteristics related to infectivity, such as enzymatic activity and animal assays. all strains hydrolysed dna, starch, gelatin and chitin. variable results were obtained with the haemolysin, chondroitin, collagen, elastin and lecithin tests. production of thermostable direct haemolysin by v. parahaemolyticus was detected in 7.1% strains derived from seafood and 2% from ... | 1993 | 8139466 |
| internalization of oligodeoxyribonucleotides by vibrio parahaemolyticus. | the bacterium vibrio parahaemolyticus was tested for its ability to internalize unmodified as well as modified dna oligomers without attempting to permeabilize the cells. these experiments were conducted to establish whether it may be feasible to employ antisense oligomers for control of gene expression in vibrio species without heat-shocking or electroporating the cells. the bacterium was found to bind radiolabeled synthetic oligodeoxyribonucleotides that were added to culture media. incorporat ... | 1993 | 8155978 |
| respiration-driven na+ pump of the marine vibrio is encoded by chromosomal dna. | a plasmid-cured strain of the marine vibrio alginolyticus 138-2 retains a respiration-driven na+ pump. examinations of several strains of v. alginolyticus and v. parahaemolyticus revealed that these murine vibrio always possessed the respiration-driven na+ pump irrespective of the presence or absence of plasmids. these results strongly suggested that the genes for the na+ pump were encoded by chromosomal dna. | 1993 | 8220320 |
| demonstration and characterization of simultaneous production of a thermostable direct hemolysin (tdh/i) and a tdh-related hemolysin (trhx) by a clinically isolated vibrio parahaemolyticus strain, th3766. | simultaneous production of a thermostable direct hemolysin (tdh)-like toxin (tdhx) and a tdh-related hemolysin (trh)-like toxin (trhx) by a clinical isolate (strain th3766) of kanagawa phenomenon-positive vibrio parahaemolyticus was demonstrated and characterized. the two hemolysins were differentially purified by column chromatography on hydroxyapatite and immunoaffinity columns. the molecular weight of the two hemolysins were estimated to be 23,000 by sodium dodecyl sulfate-polyacrylamide gel ... | 1994 | 8262624 |
| the origin of non-outbreak vibrio parahaemolyticus infections on guam. | a study of vibrio parahaemolyticus infected patients not associated with known outbreaks and controls matched for sex, ethnicity and age (+/- 5 years) was conducted on guam. cases were asked if they had eaten seafood within the 24-hour period preceding onset of illness and controls were asked if they had eaten seafood within the 24-hour period preceding their interview. cases were more likely than controls to have eaten seafood (or = 37.59, cl [8.30-220.24]). | 1994 | 8165739 |
| hemolymph lectins of the blue crab, callinectes sapidus, recognize selected serotypes of its pathogen vibrio parahaemolyticus. | 1994 | 8192339 | |
| seasonal variations in the occurrence of vibrio vulnificus along the dutch coast. | the seasonal variation in the occurrence of v. vulnificus in relation to water temperature and salinity was studied along the dutch coast. in two consecutive years v. vulnificus strains could be isolated in august when the water temperature was highest. the indole-positive strains isolated from north sea water samples were identical to most strains isolated from human disease and from the environment. however, strains isolated from four of five patients living in countries around the north sea w ... | 1994 | 8150002 |
| [a case of cardiogenic shock caused by vibrio parahaemolyticus]. | a case of a 53 year old healthy female complaining of diarrhea and abdominal pain after taking raw fish is presented. she immediately went into shock and unconsciousness. central venous pressure was 8 cmh2o and her ecg showed a first-degree av block and st-t changes in almost all leads. after mechanical ventilation and administration of dopamine, dobutamine, cefotiam, ciprofloxacin, she became alert and recovered from her critical condition. v. parahaemolyticus which produces thermostable direct ... | 1994 | 8138673 |
| isolation and sequence of an endochitinase-encoding gene from a cdna library of trichoderma harzianum. | there are no reports of gene sequences coding for extracellular chitinolytic enzymes from filamentous fungi, even though these enzymes are considered critical to the biological control of plant pathogenic fungi. the purpose of this paper was to report the isolation of a gene (then-42) encoding endochitinase (ech) from trichoderma harzianum strain p1, describe its sequence, and to determine whether it was related to genes coding for enzymes with similar functions from prokaryotic or other eukaryo ... | 1994 | 8125293 |
| effect of ionizing dose rate on the radioresistance of some food pathogenic bacteria. | food pathogenic bacteria including listeria monocytogenes (1a1 and atcc 19111), staphylococcus aureus (gd13 and atcc 13565), escherichia coli o157:h7 (atcc 35150), salmonella typhimurium, yersinia enterocolitica, vibrio parahaemolyticus, and campylobacter jejuni were exposed to various rates of ionizing radiation (0.78, 2.6, and 22 kgy/h) emitted by three different 60co irradiators. d10 values (d10 is the radiation dose required to eliminate 90% of a bacterial population (one logarithmic cycle r ... | 1994 | 8069779 |
| responses of diverse heterotrophic bacteria to elevated copper concentrations. | the influence of copper on the growth of escherichia coli, pseudomonas aeruginosa, bacillus cereus, bacillus subtilis, vibrio parahaemolyticus, vibrio alginolyticus (three strains), and an unidentified vibrio sp. was examined in batch cultures. the effects of copper at micromolar concentrations varied from undetectable to complete growth inhibition. each strain was able to recover from a growth lag observed after copper addition at a characteristic concentration. copper concentrations that allow ... | 1994 | 8069784 |
| urea hydrolysis can predict the potential pathogenicity of vibrio parahaemolyticus strains isolated in the pacific northwest. | the ability of some strains of vibrio parahaemolyticus to hydrolyze urea (uh+) can be used as a marker to predict which strains isolated from molluscan shellfish harvested in the pacific northwest are potentially pathogenic. the thermostable direct hemolysin-producing (tdh+) characteristic is a marker that is correlated with potential pathogenicity, and all of the tdh+ strains that we have isolated have been found to be uh+. most of the uh+ strains belong to somatic antigen groups o3, o4 and o5. ... | 1994 | 8085837 |
| aspects of the haemolytic reaction induced by kanagawa haemolysin of vibrio parahaemolyticus. | vibrio parahaemolyticus, an important enteric pathogen, produces toxin (kanagawa haemolysin, kh), the presence of which correlates well with pathogenicity. kh induced lysis of human red blood cells (hrbc); the kinetics were strongly dependent on kh concentration (0-1 hu/ml) and rather independent of target cell concentration [0.5 < or = haematocrit (%) < or = 6] and the ratio kh:hrbc. the suggestion that kh-induced haemolysis is due to colloid osmosis is supported by results indicating: (1) osmo ... | 1994 | 7886698 |
| properties and sequence of the nhaa na+/h+ antiporter of vibrio parahaemolyticus. | a gene encoding an na+/h+ antiporter was cloned from chromosomal dna of the slightly halophilic marine bacterium vibrio parahaemolyticus. the host was an escherichia coli mutant that lacked both of the two major na+/h+ antiporters, nhaa and nhab. untransformed mutant cells were unable to grow in the presence of 0.6 m nacl or 0.1 m licl, but na+ and li+ were non-toxic to cells transformed with a plasmid carrying the antiporter gene. membrane vesicles prepared from the original e. coli mutant did ... | 1994 | 7896730 |
| correlation between cell-associated mannose-sensitive hemagglutination by vibrio parahaemolyticus and adherence to a human colonic cell line caco-2. | cell-associated hemagglutination (cha) activity with human erythrocytes was examined for 468 clinical and 71 environmental strains of vibrio parahaemolyticus. approximately 95% of the strains tested were cha positive irrespective of source or kanagawa phenomenon. 75% of clinical strains showed relatively strong mannose-sensitive hemagglutination (msha), whereas 88% of the environmental strains showed relatively weak mannose-resistant hemagglutination (mrha). adherence of v. parahaemolyticus to c ... | 1994 | 7914500 |
| vibrio cholerae iron transport systems: roles of heme and siderophore iron transport in virulence and identification of a gene associated with multiple iron transport systems. | vibrio cholerae iron transport mutants were tested for their ability to cause disease in an infant mouse model. the mice were challenged with either the wild-type strain, a vibriobactin synthesis mutant, a heme utilization mutant, or double mutants containing both the vibriobactin synthesis defect and the heme utilization defect. when mice were challenged with 10(7) bacteria, the ability of the double mutant to survive in the intestines was greatly reduced and that of the heme utilization mutant ... | 1994 | 7927795 |
| motx, the channel component of the sodium-type flagellar motor. | thrust for propulsion of flagellated bacteria is generated by rotation of a propeller, the flagellum. the power to drive the polar flagellar rotary motor of vibrio parahaemolyticus is derived from the transmembrane potential of sodium ions. force is generated by the motor on coupling of the movement of ions across the membrane to rotation of the flagellum. a gene, motx, encoding one component of the torque generator has been cloned and sequenced. the deduced protein sequence is 212 amino acids i ... | 1994 | 7928960 |
| regulation of iron on bacterial growth and production of thermostable direct hemolysin by vibrio parahaemolyticus in intraperitoneal infected mice. | pathogenesis of vibrio parahaemolyticus is not clearly understood. effects of iron on the bacterial proliferation and production of thermostable direct hemolysin (tdh) in intraperitoneal infected mice were studied. injection of bacterial culture in the presence of ferric ammonium citrate (100 micrograms/ml) significantly enhanced the lethality for mice, and simultaneously activated bacterial proliferation in vivo. the iron-limited cultures showed better proliferation than those iron-rich culture ... | 1994 | 7935061 |
| [extraintestinal infections caused by vibrio parahaemolyticus and vibrio alginolyticus at the county of funen 1987-1992]. | over a six-year period we detected 30 clinical infections caused by halophilic vibrios in a restricted geographical area. vibrio parahaemolyticus infections were found in 13 patients (three with wound infections, ten with ear infections), and vibrio alginolyticus infections in 17 patients, all of whom had ear infections. from 1987 to 1990, infections caused by marine vibrios were found in at most four cases annually, in 1992 in six instances, whereas over a five-month period in 1991 we experienc ... | 1994 | 7941065 |
| [the ecological and epidemiological aspects of the spread of vibrio parahaemolyticus in a freshwater region]. | for the first time vibrio parahaemolyticus have been isolated in this country in a freshwater region both from patients with the diarrheal syndrome and from environmental objects. in the region of the lower course of the volga morbidity in acute enteric infections (aei) caused by these vibrios may reach 9.1% of all aei cases in some years. the formation of an ecological niche suitable for the circulation of these bacteria has taken place in the volga delta. all these facts indicate that freshwat ... | 1994 | 7941868 |
| f0f1-atpase of vibrio parahaemolyticus: purification using new detergents and characterization. | previous attempts to isolate a stable f0f1-atpase complex (h(+)-translocating atpase) from vibrio parahaemolyticus have been unsuccessful. using new non-ionic detergents (alkyl thiomaltosides), a stable f0f1 complex with a high specific activity (15-25 units/mg protein) was purified and characterized. the purified f0f1-atpase consists of eight subunits (alpha, beta, gamma, delta, epsilon, a, b and c). the new detergents, in combination with sucrose (or glycerol), lipid, dithiothreitol and phenyl ... | 1994 | 7947906 |
| characterization of a glucose transport system in vibrio parahaemolyticus. | cells of a glucose-pts (phosphoenolpyruvate:carbohydrate phosphotransferase system)-negative mutant of vibrio parahaemolyticus transport d-glucose in the presence of na+. maximum stimulation of d-glucose transport was observed at 40 mm nacl, and na+ could be replaced partially with li+. addition of d-glucose to the cell suspension under anaerobic conditions elicited na+ uptake. thus, we conclude that glucose is transported by a na+/glucose symport mechanism. calculated vmax and km values for the ... | 1994 | 7961512 |
| structure and iron transport activity of vibrioferrin, a new siderophore of vibrio parahaemolyticus. | the structure of vibrioferrin, a siderophore from vibrio parahaemolyticus, was elucidated based on a combination of partial hydrolysis and spectroscopic techniques. hplc of purified vibrioferrin showed two peaks with an area ratio of approximately 2:1. however, upon reinjection of each of those isolated compounds, the original chromatographic pattern was obtained, indicating an equilibrium between two compounds in aqueous solution. consistent with this finding, most of the nmr signals of vibriof ... | 1994 | 7961600 |