Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| isolation and identification of ropy bacteria in raw milk. | approximately 4.2% of 4,000 maryland-virginia raw milk tanker samples developed ropiness when incubated at 10 degrees c. of the 56 bacterial isolates 30 were identified by species. klebsiella oxytoca and pseudomonas aeruginosa were isolated most frequently. other ropy isolates were identified as pseudomonas spp., chromobacterium, flavobacterium multivorum, presumptive yersinia pestis, enterobacter agglomerans, klebsiella pneumoniae, and pasteurella-actinobacter spp. six of the klebsiella oxytoca ... | 1983 | 6685140 |
| use of uv-irradiated bacteriophage t6 to kill extracellular bacteria in tissue culture infectivity assays. | we have utilized 'lysis from without' mediated by uv-inactivated bacteriophage t6 to eliminate extracellular bacteria in experiments measuring the internalization, intracellular survival and replication of yersinia pestis within mouse peritoneal macrophages and of shigella flexneri within a human intestinal epithelial cell line. the technique we describe has the following characteristics: (a) bacterial killing is complete within 15 min at 37 degrees c, with a greater than 10(3)-fold reduction in ... | 1983 | 6338113 |
| ecology of plague in africa: response of indigenous wild rodents to experimental plague infection. | the mastomys natalensis species complex, subdivided into genetically distinct species having diploid chromosome numbers 2n = 32 and 2n = 36, is a reservoir for several zoonoses including lassa fever and plague. this report describes a study to determine whether these sibling species and three other rodent species have different potential as reservoirs for plague. it was found that m. natalensis (2n = 32) was significantly more resistant to experimental plague infection (50% survived inoculation ... | 1983 | 6345015 |
| [analysis of the discrete structure of antibodies by affinity and antigen-binding capacity]. | immune sera are a mixture of different groups of antibodies belonging to the same class of immunoglobulins, but differing in their affinity. the graphic analysis and numerical methods permit the determination of the size and affinity of each group of antibodies. differences in affinity are manifested as differences in antigen-binding capacity. the antigen-binding capacity index, calculated as the product of the association constant multiplied by the concentration of the active centers of antibod ... | 1983 | 6362294 |
| [epizootic process of natural focus infections from the viewpoint of systems theory]. | 1983 | 6362295 | |
| [quantitative characteristics of the survival phenomenon in experimental plague]. | the method for the quantitative evaluation of the effect of the survival phenomenon is proposed, which makes it possible to carry out the rapid single-stage determination of the immunogenicity of pasteurella pestis avirulent strains. the low specificity of the survival phenomenon in plague has been shown. the accumulation of immunogenic avirulent cells in the population of virulent p. pestis has been found to decrease their pathogenic action to a greater extent than the accumulation of nonimmuno ... | 1983 | 6362297 |
| [attempt at detecting the systems of host specificity in the bacterial causative agents of plague]. | an attempt at revealing the system of host specificity in y. pestis by the methods of transformation and conjugation has been made. no endonuclease restriction has been detected. | 1983 | 6362298 |
| [molecular organization and functions of the protein toxins of the causative agents of cholera and plague]. | 1983 | 6362301 | |
| left ventricular stiffness. | left ventricular stiffness is defined by the relationship between pressure and volume and can be altered by a variety of disorders. in disorders with high vlved, such as mitral regurgitation, protection of pulmonary capillaries from high pressure is provided by a pv shift. in contrast, increased stiffness is produced by ventricular hypertrophy or ischemic heart disease, which puts the patient at increased risk for pulmonary capillary hypertension. changing the ventricular pv relationship by ther ... | 1983 | 348043 |
| the role of the association in education for the profession. | 1983 | 264831 | |
| photoinduced crosslinking of membrane proteins by fluorescein isothiocyanate. | 1983 | 217376 | |
| evidence for the extinction of plague in hawaii. | plague (infection by yersinia pestis) was present in hawaii for the period 1899-1957 and caused at least 370 fatalities. the first infections came from immigrant commensal rats, probably rattus rattus and rattus norvegicus, on ships from the orient. both species were already established in hawaii and became the widespread local carriers of plague, supplemented by rattus exulans which had colonized the islands in ancient polynesian times. the flea xenopsylla vexabilis arrived with r. exulans, and ... | 1984 | 6364800 |
| in vivo comparison of avirulent vwa- and pgm- or pstr phenotypes of yersiniae. | the abilities of yersinia pestis to undergo restriction in ca2+-deficient medium with concomitant production of v and w antigens (vwa+) and to absorb exogenous pigments (pgm+) are established virulence factors. mutation of y. pestis to pgm- is known to promote resistance to pesticin (pstr) and reduced lethality by peripheral routes of injection. vwa+ pgm- isolates of y. pestis were shown in this study to retain virulence in mice when injected intravenously. although pgm- in appearance, wild-type ... | 1984 | 6365786 |
| growth in mouse peritoneal macrophages of yersinia pestis lacking established virulence determinants. | cultured mouse resident peritoneal macrophages were challenged with strains of yersinia pestis differing only with respect to the absence of one or more of the virulence determinants established for the species. guanine-auxotrophic (pur-) yersiniae were unable to survive within the macrophages; exogenous hypoxanthine and guanosine permitted intracellular growth. this finding supports the idea that pur- yersiniae are avirulent due to inability to obtain sufficient free purines in host tissues for ... | 1984 | 6469351 |
| yersinia pestis grows within phagolysosomes in mouse peritoneal macrophages. | transmission electron microscopy was used to determine the intracellular localization of yersinia pestis growing within cultured resident peritoneal macrophages of mice. monolayers fixed immediately, or as long as 4.5 h, after infection contained yersiniae closely surrounded by a membrane. the vesicles containing the yersiniae were determined to be phagolysosomes by labeling secondary lysosomes of macrophages with electron-dense particles of thorium dioxide. the presence of the label within vesi ... | 1984 | 6469352 |
| effect of chlorination on antibiotic resistance profiles of sewage-related bacteria. | a total of 1,900 lactose-fermenting bacteria were isolated from raw sewage influent and chlorinated sewage effluent from a sewage treatment plant, as well as from chlorinated and neutralized dilute sewage, before and after a 24-h regrowth period in the laboratory. of these isolates, 84% were resistant to one or more antibiotics. chlorination of influent resulted in an increase in the proportion of bacteria resistant to ampicillin and cephalothin, the increase being most marked after regrowth occ ... | 1984 | 6476832 |
| [method of disinfecting plague-infected fleas in preparation for electron microscopic study]. | during fixation of fleas infected with plague agent (with preliminary cut heads, limbs and posterior part of the abdomen) in 2.5% glutaric dialdehyde or 2% osmium tetroxide their disinfection was obtained in two days. after double fixation with 2-hour exposure in each of these fixators disinfection of the material was acheived only after additional 2-day maintenance in 70% ethyl alcohol. fleas, which had been placed into fixators or in 70% ethyl alcohol after double fixation without preliminary ... | 1984 | 6483471 |
| role of the virulence-associated plasmids of yersinia enterocolitica on its immunogenicity against y. pestis. | forty-two strains selected from various serovars of yersinia enterocolitica were studied for their ability to induce cross-protection against y. pestis. only primo-infected mice (i.v.) with inocula prepared from strains of y. enterocolitica belonging to serovars 0:3, 0:9 and 0:5, 27 harbouring a 47-mdal virulence-associated plasmid were resistant to y. pestis. the spontaneously cured (plasmid-less) derivatives of these strains had lost the capacity to induce this cross-immunity. | 1984 | 6532281 |
| a comparison of serological techniques for plague surveillance. | sera collected from 691 dogs and 231 rodents in south africa were tested in parallel by the passive haemagglutination (pha) and enzyme-linked immunosorbent assay (elisa) for antibodies to the fraction 1 antigen of the plague bacillus (yersinia pseudotuberculosis subsp. pestis). there was a high degree of correlation between the tests, both in over-all numbers positive and in serum titres. 42 dog sera were positive by pha and 41 by elisa, 40 sera being positive by both tests. however, using elisa ... | 1984 | 6533848 |
| cross reactions in pha test for plague antibodies. | 1984 | 6535790 | |
| human bubonic plague transmitted by a domestic cat scratch. | bubonic plague was transmitted to a 10-year-old girl in oregon by a scratch wound inflicted by a domestic cat. the cat probably was infected by contact with infected wild rodents or their fleas. yersinia pestis was identified in diamanus montanus fleas collected from an abandoned burrow near the patient's home. domestic cats may infect humans with y pestis by inoculation from a scratch. | 1984 | 6694293 |
| primary plague pneumonia contracted from a domestic cat at south lake tahoe, calif. | primary plague pneumonia occurred in a 47-year-old south lake tahoe woman shortly after face-to-face exposure to her plague pneumonia-infected cat. both died. field investigation revealed a recent plague epizootic in squirrels and chipmunks around the patient's home. control measures included active surveillance and chemoprophylaxis of 197 contacts to the victim, a community alert on methods of self- and pet protection, and application of insecticide to reduce rodent flea populations. no seconda ... | 1984 | 6694294 |
| plague and the peripheral smear. | 1984 | 6694298 | |
| [results of experiments simulating the preservation of the causative agent of plague under natural conditions]. | 1984 | 6727783 | |
| [effectiveness of revaccinating hamadryas baboons with niss live dried plague vaccine and fraction i of the plague microbe]. | the effectiveness of some vaccine preparations for the revaccination of hamadryas baboons after their primary immunization with live plague vaccine " niis " administered in the form of aerosol was studied. the study was carried out under the conditions of the aerosol challenge of the animals with y. pestis. the subcutaneous injection of plague vaccine " niis " was found to have advantages over its aerosol administration. revaccination with y. pestis fraction i, absorbed, was found to be 8 times ... | 1984 | 6741359 |
| potential for rat plague from nonencapsulated variants of the plague bacillus (yersinia pestis). | potentials for oral and flea-borne transmission of nonencapsulated y. pestis were demonstrated when 45% of rats that consumed infected meat died of plague and 22% of the rats that died of plague had bacteremia. | 1984 | 6745406 |
| genetic analysis of the low calcium response in yersinia pestis mu d1(ap lac) insertion mutants. | yersinia pestis strain kim requires plasmid pcd1 for expression of the low calcium response, plague virulence antigen v, and virulence. we constructed mu d1(ap lac) insertion mutants of this plasmid which were unable to express the low calcium response. the insertions mapped to a 17-kilobase region of the plasmid. by determining the orientation of the insertions and examining beta-galactosidase production from the mu d1 lac genes, we determined that this region contains three units of transcript ... | 1984 | 6094509 |
| [comparison of specific recognition sites of adenine and cytosine dna-methylase of yersinia pestis ev 76 c dam and dcm by escherichia coli methylases]. | using enzymatic modelling of in vitro methylation of chromosome dnas from yersinia pestis ev 76, e. coli 834 and e. coli c600 rii by dna methylases of eco rii and eco dam as well as of dna hydrolysis of plasmid pbr 322 from the cells of y. pestis ev 76, e. coli c600 and e. coli 834 by restrictases of eco rii and cfu i, it was found that cytosine dna methylase from plague bacteria does not correspond to the type of rii methylases of e. coli. adenyl dna methylase is related to e. coli methylases t ... | 1984 | 6097301 |
| [a possible way of realizing a signal of increased temperature for plague microbe cultivation]. | 1984 | 6101075 | |
| [detection of a specific plague microbe antigen (fraction i) by the method of radioimmunological analysis]. | 1984 | 6200692 | |
| characterization of common virulence plasmids in yersinia species and their role in the expression of outer membrane proteins. | the virulence plasmids pyv019, pyv8081, and pib1 from yersinia pestis, yersinia enterocolitica, and yersinia pseudotuberculosis, respectively, were characterized by restriction endonuclease analysis. the three plasmids exhibited a region of common dna previously shown to encode determinants which confer ca2+ dependence. the plasmids from y. pestis and y. pseudotuberculosis were similar throughout their genomes. in contrast, a region of the plasmid from y. enterocolitica which contained an origin ... | 1984 | 6317562 |
| [choice of the optimal technic for immunofluorescence staining using microfluorometry]. | 1984 | 6210412 | |
| [isolation, purification and physicochemical properties of pesticin i from cells of yersinia pestis strain ev]. | pesticin i has been isolated and purified from y. pestis strain ev. the homogeneous preparation of pesticin has been shown to be monomer protein with a molecular weight of 65000 daltons, having three immunologically identical alpha-, beta- and gamma-forms with different isoelectric points. the amino acid composition of pesticin i is presented. rabbit anti-serum to the beta-form of the preparation of pesticin has been obtained. | 1984 | 6431718 |
| roles of v antigen in promoting virulence and immunity in yersiniae. | it is established that yersiniae harboring an approximately 45-megadalton vwa-plasmid can produce v and w antigens (vwa+), and that sera containing anti-v provides passive protection to mice against yersinia pestis. this observation was extended by the use of monospecific anti-v prepared by injecting rabbits with partially purified v, absorption of antisera with a vwa- extract, and then separation of gamma-globulin by traditional processes of fractionation or by affinity chromatography. these pr ... | 1984 | 6381597 |
| [use of monoclonal antibodies in immunoenzyme analysis for demonstrating antigenic monovalency]. | to prove the monovalence of the antigen a method has been developed consisting of elisa with the use of monoclonal antibodies in combination with the antibody neutralization test. yersinia pestis capsular antigen was disintegrated by heating at 100 degrees c for a short time and subsequently passed through a column packed with sephadex g-50. the portions of the eluate, showing high activity in the antibody neutralization test and low activity in elisa (the double antibody sandwich scheme), conta ... | 1984 | 6395594 |
| a simple method of eminoplasty for correction of recurrent dislocation of the mandible. | a simple surgical method of eminoplasty has been described that is rapid and has few complications. its value in the treatment of recurrent dislocation of the mandible is now well established. whether the procedure works by providing greater freedom of motion or by limitation is a moot point as the operation is highly successful. | 1984 | 264267 |
| leukocyte function after aortic valve replacement. | leukocyte function was studied in patients with prosthetic heart valves by oxygen consumption measurements during phagocytosis of polystyrene latex particles. the consumption reflects the phagocytotic capacity of the cells. in 38 patients with starr-edwards aortic ball valves the mean oxygen consumption was 3.95 nanoatoms per minute per 10(6) leukocytes, as compared to 4.15 in 50 healthy subjects, the difference not being statistically significant. the number of leukocytes per ml. of blood and t ... | 1984 | 263395 |
| functional differentiation of cell types of cortical collecting duct. | interference-contrast and fluorescent microscopy were used to differentiate between the two cell types--principal cells (pc) and intercalated cells (ic)--of the isolated perfused cortical collecting duct of the rabbit. using hoffman modulation contrast optics, two types of cell outlines could be identified: "hexagonal" and "circular" profiles. to characterize the cell types further, the binding of fluorescein-labeled peanut lectin, which has been shown to be specific for the luminal cell membran ... | 1985 | 2579583 |
| all hepatocytes are involved in the expression of the albumin gene in the normal adult rat: a demonstration by in situ hybridization and immunoperoxidase techniques. | immunoperoxidase techniques have yielded conflicting results concerning the percentage of hepatocytes engaged in albumin production in normal adult rats. in addition, the question of whether functional differences in the synthesis of plasma proteins exist within the hepatic lobule remains to be determined. to clarify these questions, we have searched for gene albumin transcripts by in situ hybridization, and for the corresponding protein by immunoperoxidase, on adjacent liver sections. we observ ... | 1985 | 2579741 |
| ion selectivity of gram-negative bacterial porins. | twelve different porins from the gram-negative bacteria escherichia coli, salmonella typhimurium, pseudomonas aeruginosa, and yersinia pestis were reconstituted into lipid bilayer membranes. most of the porins, except outer membrane protein p, formed large, water-filled, ion-permeable channels with a single-channel conductance between 1.5 and 6 ns in 1 m kcl. the ions used for probing the pore structure had the same relative mobilities while moving through the porin pore as they did while moving ... | 1985 | 2580824 |
| [basic somatic antigen or antigen obtained from yersinia pestis by the boivin-mesrobeanu method]. | 1985 | 2581401 | |
| expression of an hla-bw6-related specificity by the hla-cw3 molecule. | radioimmunoassay of hla-transformed mouse l cells expressing a3, a24, b7, or cw3 hla class i molecules with a set of monomorphic monoclonal antibodies distinguishes between a3-a24 and b7-cw3 patterns of reactivity. analyses with bw6-specific monoclonal antibodies and a human alloantiserum demonstrate the expression by the hla-cw3 molecules of a bw6 public specificity related to but not identical with that expressed by the hla-b7 molecules. exon-shuffling experiments and inhibition studies of mon ... | 1985 | 2581891 |
| an evaluation of the combined use of nuclear magnetic resonance and distance geometry for the determination of protein conformations in solution. | an evaluation of the potential of nuclear magnetic resonance (n.m.r.) as a means of determining polypeptide conformation in solution is performed with the aid of a new distance geometry program which is capable of computing complete spatial structures for small proteins from n.m.r. data. ten sets of geometric constraints which simulate the results available from n.m.r. experiments of varying precision and completeness were extracted from the crystal structure of the basic pancreatic trypsin inhi ... | 1985 | 2582141 |
| [use of hemin as a stimulator of the growth of the plague microbe]. | 1985 | 2582200 | |
| a projection from the deep layers of the entorhinal area to the hippocampal formation in the rat brain. | the methods of retrograde fluorescent tracing and anterograde transport of the lectin phaseolus vulgaris leucoagglutinin (pha-l) were used to demonstrate the existence of projections from layers iv and vi of the entorhinal area to the hippocampal formation in the rat brain. these two layers of the medial and lateral entorhinal area innervate the molecular layer of ammon's horn and the area dentata. in the area dentata the projection from layer iv follows that of the perforant path, while that fr ... | 1985 | 2409482 |
| a study on the pharmacokinetics in mouse of adenine-9-beta-d-arabinofuranoside 5-monophosphate conjugated with lactosaminated albumin. | in plasma of mice injected with adenine-9-beta-d-arabinofuranoside monophosphate (ara-amp) coupled to human lactosaminated serum albumin (l-hsa) some of the ara-amp molecules are enzymatically released, whereas others remain linked to l-hsa. evidence has been obtained that ara-amp is not deaminated when it is conjugated to l-hsa, in contrast to the free drug which is rapidly metabolized to its hypoxanthine derivative. | 1985 | 2412878 |
| monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species. | the highly reproducible histochemical localization of glial fibrillary acidic protein (gfap)+ qualifies it as an important marker of astrocytes in both research and clinical applications. the primary objective of this study was to produce monoclonal antibodies having the advantage of invariant specificity, affinity, and titer to gfap-specific epitopes of wide species distribution. we report here the characterization of four monoclonal antibodies that recognize the same or spatially close epitope ... | 1985 | 2413405 |
| [vital staining of the conjunctiva for prevention of epithelial implantation cysts]. | selective vital staining with 1% toluidine blue makes short-term intraoperative differentiation of the conjunctiva from tenon's capsule possible. it is used with advantage in operations where the anatomical situation is unclear to avoid displacement of conjunctival epithelium into deeper tissues and subsequent growth of cysts. examples of its application are reoperations with excessive scars after squint or detachment surgery, wound dehiscence after enucleations, lacerated wounds after accidents ... | 1985 | 2415747 |
| [requirements and standardization of the conditions for carrying out the microfluorometric test]. | 1985 | 2415765 | |
| prostanoids and adrenaline release: a study of [3h]adrenaline efflux from the rabbit isolated, perfused, adrenal gland. | [3h]adrenaline was incorporated in an isolated perfused preparation of the rabbit adrenal gland and the effects of indomethacin, pge2 and pgi2 on its release were investigated. efflux of [3h]adrenaline was elicited by electrical stimulation of the splanchnic nerve (60 s at 5 hz). indomethacin (3 and 30 microm) had no effect on stimulation-induced efflux. pge2 (30, 90 and 300 nm) reduced the efflux; with 90 nm pge2 the inhibition amounted to approximately 30%. pgi2, in concentrations from 90 to 6 ... | 1985 | 2858566 |
| prolactin and its involvement in fluid regulation in the bovine. | plasma prolactin (prl) concentrations, osmolality, water consumption, feed intake, urine excretion, and fecal water output were determined in twelve steers of 3 breeds exposed to 5 feed and water regimes. breed differences were found in water intake and plasma prl concentrations when feed and water were ad lib., however, during any of the other 4 treatments, responses were similar between breeds. during dehydration and feed restriction, water intake, urine, fecal water, and plasma prolactin decr ... | 1985 | 2859961 |
| a role for amniotic epithelium in control of human parturition. | a peroxidase anti-peroxidase staining technique was used to determine the presence of pregnancy-associated prostaglandin synthetase inhibitor (papsi) in human amniotic epithelium from 20 pregnant women at 36-40 weeks' gestation. papsi was present in amniotic epithelial cells in 12 of 15 women before the onset of labour. no papsi was found in amniotic epithelium from 5 women in clinically established labour. these findings indicate that the amnion is a source of this endogenous prostaglandin synt ... | 1985 | 2860289 |
| lav/htlv-iii antibodies in connective tissue diseases. | 1985 | 2863569 | |
| combination of mefloquine with sulfadoxine-pyrimethamine compared with two sulfadoxine-pyrimethamine combinations in malaria chemoprophylaxis. | 1985 | 2863679 | |
| [adhesion pili in yersinia pestis]. | y. pestis cells cultivated at 37 degrees c are capable of agglutinating red blood cells of some animals, which is due to the appearance of pili. the adhesion pili consist of protein subunits with a molecular weight of the order of 12000 daltons, their isoionic point being at ph 4.7. the reaction of hemagglutination was inhibited by the mixture of ganglyosides, while the preliminary treatment of red blood cells with neuraminidases increased its effectiveness. the pili are supposed to take part in ... | 1985 | 2863905 |
| neurohumoral vasoconstrictor systems in heart failure. | by means of specific inhibitors of the renin-angiotensin system (captopril) and of the sympathetic nervous activity (prazosin) in dogs with congestive heart failure, and by using a specific antagonist of the pressor activity of arginine vasopressin in rats with heart failure, we studied the influence of these pressor hormone systems on peripheral vascular resistance and cardiac function. all three humoral vasoconstrictor systems were stimulated in heart failure. the experiments in dogs showed th ... | 1985 | 2864248 |
| characterization of depolarization induced by palytoxin and grayanotoxin-i in isolated cardiac tissues from dogs and guinea pigs. | the mode of depolarizing action of palytoxin (ptx) was compared with that of grayanotoxin-i (gtx-i) to examine the site of action of ptx in cardiac tissues isolated from dogs and guinea pigs: ptx at above 1 x 10(-10) mol/l depolarized the membrane of canine purkinje fibres, canine and guinea-pig ventricular muscles regardless of stimulation. the ptx-induced depolarization was resistant to 1 x 10(-5) mol/l tetrodotoxin (ttx) but was attenuated by low na medium. gtx-i at 1 x 10(-5) mol/l depolariz ... | 1985 | 2864640 |
| mechanism of tachycardia caused by intracarotid pge2 in conscious ewes. | conscious adult ewes prepared with nonocclusive indwelling vascular catheters were used to determine the mechanism by which heart rate increases during central administration of prostaglandin e2 (pge2). heart rate increased 14 bpm during steady-state intracarotid infusion of pge2, 10 ng/kg/min (p less than 0.05). intravenous atropine methyl bromide, 1 mg/kg, increased heart rate 26 bpm (p less than 0.05) 5 min after injection. heart rate remained elevated 30 min after injection. the heart rate r ... | 1985 | 2864696 |
| angiogenin. | 1985 | 2866393 | |
| trials with portable screen rooms modified for use as animal-baited net traps for mosquito collection. | trials in larimer county, colorado during july and august 1984, with recreational screen rooms modified as large animal-baited mosquito traps are described. the two units tested are free-standing, portable and require no external support. in all-night trials, 462.5 mosquitoes/trap night were captured with horse bait compared with 367/trap night with cdc light traps. in 2-hour evening comparisons, mosquitoes collected per trapping period totalled 416 for horse-bait traps, 132 for light traps, and ... | 1985 | 2906667 |
| [comparisons of vasoconstrictions induced by alpha agonists either in the luminal perfusate or in the adventitial superfusate]. | 1985 | 2869558 | |
| [emergency cardiac pacing via a coronary vessel during percutaneous coronary angioplasty]. | percutaneous coronary angioplasty (pca) is sometimes complicated by bradyarrhythmias necessitating emergency temporary cardiac pacing. this is usually performed by the classical transvenous endocardial approach. this experimental study investigated the possibility of using the metallic guide wire used during pca as a monopolar electrode. systematic electrical stimulation at different levels of the coronary arteries in 6 anaesthetised pigs showed threshold levels in the distal segments of 3 to 15 ... | 1985 | 2937382 |
| thyroxine induced transformation in sarcoplasmic reticulum of rabbit soleus and psoas muscles. | the properties of the sarcoplasmic reticulum membranes isolated from slow-twitch type i soleus and fast-twitch type ii psoas muscles of control and thyroxine treated rabbits were comparatively studied. membrane yield, maximal calcium storing capacity, atp-supported calcium uptake, calcium-dependent atpase activity and calcium-dependent phosphoprotein formation were found to be 3-10 fold higher in psoas than in soleus preparations. membrane yield, calcium-dependent atpase activity, atp-supported ... | 1985 | 2934902 |
| [influence of pyronaridine phosphate on three-generation reproduction in rats]. | 1985 | 2934948 | |
| studies on the atp-sensitivity of yeast phosphofructokinase by means of affinity partitioning using polymer bound cibacron blue f3g-a. | yeast phosphofructokinase was partitioned in an aqueous two-phase system composed of polyethylene glycol and dextran in which a small amount of the first polymer was replaced by cibacron blue f3g-a substituted polyethylene glycol. it was found that the partition coefficient, k, of the enzyme determined immediately after adding the enzyme to the system and within a series of time intervals (min) shifts from a lower to a higher value. this effect was amplified when the enzyme was preincubated with ... | 1985 | 2935143 |
| [interaction of yersinia pestis bacteria with bacteriophage mu]. | yersinia pestis cells are shown to be sensitive to bacteriophage mu cts62 infection. lysis of bacteria has been shown to be more efficient on solid nutrient medium than in lb broth. 10(-5) pfu per ml is the maximal concentration of bacteriophage particles yielded from the broth cultures of bacteria. moi 0.1 has been used to obtain such yields of bacteriophage. lysogenization of yersinia pestis cells has not been achieved when the standard methods of bacteriophage infection have been used. it was ... | 1985 | 2948125 |
| cosmid cloning of rickettsia prowazekii antigens in escherichia coli k-12. | rickettsia prowazekii dna was partially digested with sau3a or hindiii, ligated with the cosmid vector phc79, packaged in vitro, and transduced into escherichia coli hb101. cosmid cloning of sau3a-digested rickettsial dna yielded 1,288 ampicillin-resistant colonies; 798 cosmid clones resulted with hindiii-digested rickettsial dna. chimeric cosmid dna was extracted from the latter gene bank, digested to completion with hindiii, and compared by agarose gel electrophoresis with a hindiii digest of ... | 1985 | 2981194 |
| corticotropin-releasing factor desensitization of adrenocorticotropic hormone release is augmented by arginine vasopressin. | the desensitization of corticotropin-releasing factor (crf)-stimulated acth release from and intracellular cyclic amp accumulation in anterior pituitary cells was investigated in primary cultures of rat pituitary cells and in a mouse tumor cell line (att 20/d16-16). crf potently stimulated acth secretion and cyclic amp accumulation in both preparations. when primary cultures were preincubated with 100 nm crf, 50% desensitization of acth release occurred within 4 hr while similar desensitization ... | 1985 | 2981299 |
| viremia is present in incubation period in nonimmunocompromised children with varicella. | 1985 | 2981309 | |
| uridine 5'-diphosphate glucose analogues. inhibitors of protein glycosylation that show antiviral activity. | a series of analogues of uridine 5'-diphosphate glucose and uridine 5'-diphosphate glucosamine have been synthesized by reaction of 2,3,4,6-tetra-o-benzyl-, 2,3,4,6-tetra-o-benzoyl-, 2,3,4,6-tetra-o-acetyl-, and 2,3,4,6-tetra-o-palmitoyl-alpha-d-glucopyranose and 2-acetamido-3,4,6-tri-o-acetyl-2-deoxy-alpha-d-glucopyranose with chlorosulfonyl isocyanate and 2',3'-o-isopropylideneuridine. isopropylidene and acetyl groups of the resulting 5'-o-[[[[(alpha-d-glucopyranosyl)oxy]carbonyl]amino]sulfony ... | 1985 | 2981323 |
| middle tumor antigen of polyomavirus transformation-defective mutant ng59 is associated with pp60c-src. | we have found that lysis of mouse embryo cells infected with the polyomavirus host range transformation-defective (hr-t) mutant ng59 under gentle conditions that avoid ionic detergents results in detectable ng59-encoded middle tumor antigen (mtag) associated with pp60c-src. this mtag-pp60c-src complex could be immunoprecipitated from ng59-infected cell lysates by either sera from animals bearing polyomavirus-induced tumors or by monoclonal antibodies directed against mtag. immune complex kinase ... | 1985 | 2981329 |
| effect of acute acrylonitrile exposure on metrazol induced seizures in the rat. | the effects of acute exposure to acrylonitrile (acn), 10, 20, or 40 mg/kg by gavage, on the ability of metrazol (mtz) to induce seizures was studied in adult, male sprague-dawley rats. the frequency of seizure occurrence and the frequency of a lethal seizure was greater when the high acn dosage was given in combination with metrazol. this dosage of acn was not lethal when given alone. examination of brain tissue in these animals revealed no difference in cyanide levels when mtz was combined with ... | 1985 | 2986062 |
| effect of acute acrylonitrile exposure on metrazol induced seizures in the rat. | the effects of acute exposure to acrylonitrile (acn), 10, 20, or 40 mg/kg by gavage, on the ability of metrazol (mtz) to induce seizures was studied in adult, male sprague-dawley rats. the frequency of seizure occurrence and the frequency of a lethal seizure was greater when the high acn dosage was given in combination with metrazol. this dosage of acn was not lethal when given alone. examination of brain tissue in these animals revealed no difference in cyanide levels when mtz was combined with ... | 1985 | 2986062 |
| respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mrna, identification of cleavage activation site and amino acid sequence of n-terminus of f1 subunit. | the amino acid sequence of respiratory syncytial virus fusion protein (fo) was deduced from the sequence of a partial cdna clone of mrna and from the 5' mrna sequence obtained by primer extension and dideoxysequencing. the encoded protein of 574 amino acids is extremely hydrophobic and has a molecular weight of 63371 daltons. the site of proteolytic cleavage within this protein was accurately mapped by determining a partial amino acid sequence of the n-terminus of the larger subunit (f1) purifie ... | 1985 | 2987829 |
| electron microscopic assessment of cervical punch biopsies in women followed-up for human papillomavirus (hpv) lesions. | cervical punch biopsies from 102 women prospectively followed-up for hpv (human papillomavirus) lesions (flat, inverted and papillomatous condylomas) were assessed on transmission electron microscopy (tem) for the presence of hpv particles. the virus discovery rate was correlated with the other characteristics of significance to the clinical behaviour (natural history) of these lesions, i.e. the colposcopic pattern, expression of papillomavirus genus specific structural antigens (demonstrated by ... | 1985 | 2988479 |
| pulsed electromagnetic fields promote collagen production in bone marrow fibroblasts via athermal mechanisms. | primary and passaged cultures of fibroblasts (rbmfs) raised from the bone marrow stroma of young rabbits were treated with pulsed electromagnetic fields (pemfs) from the start of each culture until 1 week after they became confluent. the pemf treatment had no effect on cell proliferation, estimated by phase contrast microscopy, by 3h-thymidine incorporation into dna, or by total dna assay. collagen production, estimated by conversion of 3h-proline to 3h-hydroxyproline in nondialyzable material w ... | 1985 | 2988718 |
| specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells. | the hemopoietic growth factor granulocyte-macrophage colony-stimulating factor, gm-csf, specifically controls the production of granulocytes and macrophages. this report describes the binding of biologically-active 125i-labeled murine gm-csf to a range of hemopoietic cells. specific binding was restricted to murine cells and neither rat nor human bone marrow cells appeared to have surface receptors for 125i-labeled gm-csf. 125i-labeled gm-csf only appeared to bind specifically to cells in the my ... | 1985 | 2990915 |
| adrenergic receptors: possible mechanism of inverse regulation of alpha- and beta-receptors. | many physiologic and pathologic conditions, including bronchial asthma, are associated with inverse changes in alpha- and beta-receptor-mediated responses in various tissues. the direction of the change elicited by a given stimulus is tissue specific, as exemplified by the actions of thyroid hormones: in the rat heart, hypothyroidism reduces beta- and increases alpha-receptor responses, whereas in the rat liver it has the opposite effects. a similar increase in beta- and decrease in alpha-recept ... | 1985 | 2991359 |
| the ultrastructural basis for synaptic transmission between primary muscle afferents and neurons in clarke's column of the cat. | the synaptic connection between primary muscle afferents and dorsal spinocerebellar tract (dsct) neurons has been studied in an attempt to reveal some of the mechanisms underlying excitatory transmission in the mammalian central nervous system. previous electrophysiological experiments have shown that the excitatory postsynaptic potentials (epsps) evoked dsct neurons by impulses in a single muscle afferent fluctuate in amplitude. these fluctuations occur between discrete amplitudes which are sep ... | 1985 | 2991483 |
| the ultrastructural basis for synaptic transmission between primary muscle afferents and neurons in clarke's column of the cat. | the synaptic connection between primary muscle afferents and dorsal spinocerebellar tract (dsct) neurons has been studied in an attempt to reveal some of the mechanisms underlying excitatory transmission in the mammalian central nervous system. previous electrophysiological experiments have shown that the excitatory postsynaptic potentials (epsps) evoked dsct neurons by impulses in a single muscle afferent fluctuate in amplitude. these fluctuations occur between discrete amplitudes which are sep ... | 1985 | 2991483 |
| diverse involvements of ni protein in superoxide anion production in polymorphonuclear leukocytes depending on the type of membrane stimulants. | effects of islet-activating protein (iap) were examined to assess the involvement of the guanine nucleotide-binding regulatory protein responsible for inhibition of adenylate cyclase system (ni protein) in the superoxide anion (o-2) production in polymorphonuclear leukocytes (pmnl) stimulated with various agents. n-formyl-methionyl-leucyl-phenylalanine (fmlp)-stimulated o-2 production was inhibited by the pretreatment with iap. o-2 production induced by each of phorbol myristate acetate, concana ... | 1985 | 2992509 |
| rates of cholesterol synthesis and low-density lipoprotein uptake in the adrenal glands of the rat, hamster and rabbit in vivo. | the absolute rate of cholesterol acquisition from de novo synthesis and from receptor-dependent and receptor-independent low-density lipoprotein (ldl) uptake was determined in the adrenal glands of the rat, hamster and rabbit under in vivo conditions. the rate of incorporation of [3h]water into cholesterol in the adrenal gland was much higher in the hamster (1727 nmol/h per g) and rabbit (853 nmol/h per g) than in the rat (71 nmol/h per g). assuming that 23 atoms of 3h are incorporated into the ... | 1985 | 2992599 |
| delay in platelet recovery after bone marrow transplantation: impact of cytomegalovirus infection. | the effect of cytomegalovirus (cmv) infection on hematopoietic recovery after marrow-ablative chemoradiotherapy followed by autologous bone marrow transplantation (bmt) was studied in patients with non-hodgkin's lymphoma of high-grade malignancy and in patients with acute leukemia. the recovery of platelets after autologous bmt occurred significantly quicker in cmv-negative patients than in cmv-positive patients (platelets greater than 50,000 per cubic millimeter after 21 1/2 v 40 days, respecti ... | 1985 | 2994781 |
| results of investigations of the cell surface membranes composition of cells transformed by td-mutant of rsv. | 1985 | 2995158 | |
| insertion of dna sequences into the human chromosomal beta-globin locus by homologous recombination. | a 'rescuable' plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human beta-globin locus. the planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed. | 1985 | 2995814 |
| isolation and characterization of the human pulmonary surfactant apoprotein gene. | pulmonary surfactant is a phospholipid-protein complex which serves to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. inadequate levels of surfactant at birth, a frequent situation in premature infants, results in respiratory failure. in all species examined, surfactant is composed primarily of dipalmitoylphosphatidylcholine and two major protein species of relative molecular mass (mr) 32,000 (32k) and 10k (refs ... | 1985 | 2995821 |
| calcitonin receptors in the rat mesencephalon mediate its analgesic actions: autoradiographic and behavioral analyses. | autoradiographic analyses of salmon calcitonin (sct) binding in the rat mesencephalon revealed an exceptionally high concentration of receptors in the ventral and ventrolateral segments of the periaqueductal gray matter (pag) extending along the entire rostral-caudal axis. relatively heavy labeling was also seen along a band extending ventrolaterally through the mesencephalic reticular formation. other receptor-rich areas include the nucleus linearis, pars compacta and lateralis of the substanti ... | 1985 | 2996694 |
| mutations affecting gyrase in haemophilus influenzae. | mutants separately resistant to novobiocin, coumermycin, nalidixic acid, and oxolinic acid contained gyrase activity as measured in vitro that was resistant to the antibiotics, indicating that the mutations represented structural alterations of the enzyme. one novr mutant contained an altered b subunit of the enzyme, as judged by the ability of a plasmid, pnov1, containing the mutation to complement a temperature-sensitive gyrase b mutation in escherichia coli and to cause novobiocin resistance ... | 1985 | 2997115 |
| isolation and characterization of ca2+-blind mutants of yersinia pestis. | the plasmid pcd1 is required for expression of the low-calcium response (lcr), virulence, and production of v antigen in yersinia pestis kim. five independent mutants constitutive for the lcr at 37 degrees c (lcrc) were obtained through ethyl methanesulfonate mutagenesis followed by ampicillin enrichment. a sixth, spontaneous mutant was obtained directly through ampicillin enrichment. these mutants failed to grow at 37 degrees c regardless of calcium concentration and produced v antigen constitu ... | 1985 | 2997127 |
| rflp for the human apolipoprotein b gene: ii;ecori. | 1985 | 2997725 | |
| [effect of tunicamycin on human cytomegalovirus replication]. | 1985 | 2998078 | |
| [effect of levamisole on the course of chronic cytomegalovirus infection in women]. | the therapeutic effect of orally given levamisole in the treatment of women with chronic cytomegalovirus infection was studied. the patients were examined serologically, cytologically, virologically; the factors of cell-mediated immunity were also studied. oral therapy with levamisole resulted in a definite increase of the cell-mediated immunity with a trend for decrease in antibody titres and reduced virus excretion. evaluation of the clinical effect of levamisole therapy, however, revealed no ... | 1985 | 2998093 |
| evaluation of a photolabile derivative of 1,25-dihydroxyvitamin d3 as a photoaffinity probe for 1,25-dihydroxyvitamin-d3 receptor in chick intestinal cytosol. | we evaluated the viability of 1 alpha, 25-dihydroxyvitamin d3-3 beta-[n-(4-azido-2-nitrophenyl)glycinate] (1,25-(oh)2-d3-ang), an analog of 1 alpha, 25-dihydroxyvitamin d3 (1,25-(oh)2-d3) as a photoaffinity probe for 1,25-(oh)2-d3 receptor in chick intestinal cytosol. a competitive-binding assay revealed that chick intestinal cytosolic 1,25-(oh)2- d3 receptor bound to 1,25-(oh)2-d3-ang approximately 20-times less effectively than it did to 1,25-(oh)2-d3. irradiation of 1,25-(oh)2-d3- ang in the ... | 1985 | 2998355 |
| autoxidation reactions of hemoglobin a free from other red cell components: a minimal mechanism. | rates of autoxidation reactions are determined for normal human hemoglobin a preparations which are extensively purified to remove all other redox active red cell components. the effects of superoxide dismutase, catalase, and hydroxyl radical scavengers on the reaction provide evidence for superoxide formation as the rate determing step followed by fast reactions that involve peroxide and hydroxyl radical. these results support a minimum overall mechanism for heme iron(ii) oxidation and dioxygen ... | 1985 | 2998382 |
| nucleotide sequence of the central non-transcribed spacer region of physarum polycephalum rdna. | in physarum polycephalum the rrna genes are present on linear extrachromosomal molecules, each containing two transcription units arranged as a giant palindrome. in the center of the molecule, between the two transcription units, is the 23-kb central spacer, previously shown to contain the replication origins and several regions of direct and inverted repeats. segments of all the repeats in the spacer have been sequenced and their overall organization determined. the entire spacer consists of re ... | 1985 | 3005112 |
| [isolation of yersinia pestis plasmids with transposon markers]. | transposons tn1, tn7, tn9, tn10 have been inserted into each of three known plasmids in yersinia pestis and have been shown to mutagenize the different plasmid genes. the marked plasmids are shown to be transduced by bacteriophage p1 cml clr 100 ts in intrageneric crosses. the genes of 61-65 md plasmid were found to be impaired with high frequencies by tn9 and tn10 insertions blocking the synthesis of fraction i antigen. the genes are also impaired in course of transduction of transposon marked ... | 1985 | 3025677 |
| [mutagenic effect during transduction of (gm-km)r markers of the r323 plasmid in yersinia pestis]. | genes of resistance to some aminoglycoside antibiotics from plasmid r323 were transduced by bacteriophage p1 cml clr100 ts in yersinia pestis. the resistance markers were capable of insertion into the chromosome or plasmid in the recipient cells causing the mutagenic effect. the results obtained suggest the transposon nature of plasmid fragment coding for gentamicin-kanamycin resistance. | 1985 | 3025695 |
| pyrimidinones. 1. 2-amino-5-halo-6-aryl-4(3h)-pyrimidinones. interferon-inducing antiviral agents. | interferon induction and antiviral activity was discovered with 2-amino-5-bromo-6-phenyl-4(3h)-pyrimidinone. an analogue study incorporating a series of 2-amino-5-substituted-6-arylpyrimidinones revealed that the most potent interferon inducers were mono- and difluorophenyl analogues. these same analogues were also potent antiviral agents against semliki forest virus and herpes simplex type 1. in addition the monomethoxyphenyl analogues were potent antiviral agents but weak interferon inducers. ... | 1985 | 2999405 |
| collagen accumulation can continue with decreased prolyl hydroxylase activity in the liver. | a single dose of dimethylnitrosamine dose-relatedly increased total hepatic hydroxyproline content in rats 14 days after the dosing. in cases of 35 mg/kg of dimethylnitrosamine, it increased rapidly to 1.7 times the normal level within 14 days. this increase persisted thereafter until 84 days. hepatic collagen prolyl hydroxylase activity was 1.8 times the normal level by the fourth day after the dosing but normalized within 14 days. it decreased further to levels significantly lower than those i ... | 1985 | 2999546 |
| the effects of castration and/or methimazole feeding on the pituitary response to temperature extremes by cockerels. | the pituitary of goitrogen-treated white leghorn cockerels is smaller in size than control birds and the pituitaries of castrated cockerels is nearly twice the size of control birds. the pituitary cells generated by these treatments may not be functional thyrotrophs or gonadotrophs and may not be able to respond to their usual stimuli. low ambient temperature is a well-known stimulus to the thyroid gland acting through pituitary tsh. cyclic-amp-dependent protein kinase activity levels are used h ... | 1985 | 3000864 |