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a craniometric study of the black and white colobus illiger 1811 (primates: ceropithecoidea).this study examines the craniometry of black and white colobus monkeys using 1072 specimens representing all the recognized subspecies (after rahm, '70) of the genus. seventy-six measurements were taken on each individual, and examined using canonical variates analysis and clustering by ward's error sum method. the assumptions of the analytical techniques are shown to be met, and the results demonstrated to be stable. examination of the populations for statistical difference and taxonomic distin ...1979114059
lipid a from endotoxin: antigenic activities of purified fractions in liposomes.isolation of lipid a by acid hydrolysis of shigella flexneri lipopolysaccharide resulted in a product that consisted of a heterogeneous mixture of bands when visualized by thin layer chromatography. differential extraction with ethyl acetate and chloroform, or extraction with edta, followed by chloroform-methanol-water (bligh-dyer extraction), or a combination of both extraction schemes, resulted in partial purification of immunologically active lipid a. eight fractions were purified further by ...1979115918
effects of methamphetamine and scopolamine on variability of response location.methamphetamine and scopolamine were studied in monkeys responding under a multiple fixed-ratio fixed-interval schedule of reinforcement. a response on any one of six levers could satisfy the schedule requirements. variability of response location was evaluated in terms of switches, where a switch was defined as a response on one lever followed by a response on a different lever. under baseline conditions the fixed-ratio schedule generated a high rate of responding and a low level of variability ...1979115956
sensitization of myelinated nociceptive afferents that innervate monkey hand. 1979115969
sugar hydrolases and their arrangement on the rat intestinal microvillus membrane.the arrangement of the sugar hydrolases, sucrase-isomaltase, maltase, and lactase on the microvillus membrane of rat intestine was investigated by immunological technique. the enzymes were purified essentially free of each other to near homogeneity and antisera of high specificity were obtained against each. microvillus membranes were prepared routinely in high purity from rat intestine and contained an average 61% protein, 20% lipid, and 19% carbohydrate, with the sugar hydrolases comprising an ...1979116006
folacin deficiency and requirement in the squirrel monkey (saimiri sciuresus).acute folacin deficiency was studied in eight young squirrel monkeys (saimiri sciureus). half of the animals were fed a semipurified deficient diet (no added folic acid) and half were fed a control diet (0.84 mg of added folic acid per kilogram of dry diet). monkeys fed the deficient diet lost weight and suffered from diarrhea and dehydration leading to the death of one of the animals after 6 weeks. folacin deficiency also was studied in six older animals fed diets containing varying levels of a ...1979116540
accidental contamination of diagnostic cultures of mycobacteria and phage identification of the contaminating strain.phage typing of mycobacteria is a new technique not yet widely used for classification and intraspecies differentiation. systematic long-term studies organized by the who have succeeded, using a battery of 11 different mycobacterial phages, in dividing m. tuberculosis into 3 different phage sub-groups, preliminarily labelled as a, b and c. a special case of phage-typing is presented which enabled identification of a virulent mycobacterial strain causing accidental contamination of diagnostic myc ...1979117577
[the effect of lh releasing hormone on testosterone levels in the blood of boars with sexual dysfunctions].the levels were assessed of testosterone in the blood plasma before and 90 minutes after i.v. application of 1 mg of synthetic lh-releasing hormone to 57 boars with disorders of sexual functions and to 43 boars without sexual dysfunctions. the group of animals with sexual disorders included boars with inferior ejaculate quality and low fertility (24 animals) and cases with disturbed sexual potency (33 boars). in animals with the studied changes of sexual functions, compared with boars without se ...1979117601
the progressive nature of neoplastic transformation of syrian hamster embryo cells in culture. 1979119980
annotations on the measurement of liver ferritin, especially in the rat. 1979120687
[evaluation of the myeloculture by puncture of the ilium crest for isolation of brucella from patients with brucellosis].hemocultures and myelocultures of samples obtained from the ilium crest were made simultaneously of 61 patients with serologic diagnosis of brucellosis. five strains were isolated by both methods and 18, by myeloculture only. all samples from which brucella were isolated by hemoculture were also positive by myeloculture. the advantages of making myelocultures of patients previously treated with antibiotics are emphasized.1979122390
a receptor for polymerized human and chimpanzee albumins on hepatitis b virus particles co-occurring with hbeag.a receptor for polymerized human serum albumin was demonstrated on dane particles as well as on 20-nm hepatitis b surface antigen particles, isolated from asymptomatic carriers of hepatitis b virus who were positive for hbeag. in contrast, such receptor was not born by 20-nm hepatitis b surface antigen particles obtained from carriers positive for antibody to hbeag. hepatitis b surface antigen particles with the receptor were heavier than those without, and when treated with pronase, they became ...1979103774
shigellosis and escherichia coli diarrhea: relative importance of invasive and toxigenic mechanisms.shigellae and dysentery-like escherichia coli must invade the epithelium of the colon to cause disease which can present as dysentery, diarrhea, or both. this paper addresses the possible role of a shigella dysenteriae-like (shiga-like) toxin in the pathogenesis of shigellosis and e. coli diarrheal diseases. the possibility for such a role is suggested by the following observations: 1) diarrhea, considered to be a result of secretion of water by the small bowel, is frequently observed in shigell ...1979104615
quantitative determination of trinitroglycerin in human plasma.we developed a simplified method for quantitative measurement of trinitroglycerin in human plasma using hexane extraction and analysis by gas-liquid chromatography with electron-capture detection. this assay was linear from 0.5-60 ng/ml. sensitivity and reproducibility were +/- 0.5 ng/ml. we used this assay to evaluate the pharmacodynamics of trinitroglycerin in 14 patients. maximum plasma levels were similar with trinitroglycerin given by constant intravenous infusion (1.6 +/- 0.4 ng/ml (sem)), ...1979104804
demonstration of a rabbit cell surface ig that bears light chain and vh9, but lacks mu-, alpha-, and gamma-allotypes-rabbit igd? 1979105044
implications for adult roles from differential styles of mother-infant bonding: an ethological study.ethological observations of maternal and infant behaviors of nine vervet monkey pairs (cercopithecus aethiops sabaeus) showed the effects of differential styles of early maternal responsiveness on later infant competence. those infants receiving the least amount of maternal responsiveness and the most time-off the mother in the first 3 months of development were more socially competent at 6 months of age. the results are discussed within current ethological "attachment" theories. the detachment ...1979105083
leukemia in workers exposed to ethylene oxide.ethylene oxide, a gaseous sterilant extensively used within health care facilities, is known to be a mutagen in bacteria and in human lymphocytes. the environmental protection agency as well as the national institue of occupational safety and health have recently stipulated certain conditions for the use of ethylene oxide despite the lack of case reports or epidemiologic studies concerning carcinogenicity. we report three cases of leukemia that occurred between 1972 and 1977 in a relatively smal ...1979105157
production of antivenom against detoxified taipoxin and immunochemical analysis of the alpha, beta and gamma subunits. 1979105431
the respiratory chain of hydrogenomonas h16.the respiratory chain of hydrogenomonas h16 is shown to consist of two pathways, one of which has as its oxidase and only cytochrome a high-potential b-type cytochrome which is concluded to be cytochrome o. the km values for the oxygen of the two pathways are consistent with the high-potential b-type cytochrome functioning as a relatively high-affinity oxidase.1979107951
rna replication: required intermediates and the dissociation of template, product, and q beta replicase.replication complexes containing only one molecule of q beta replicase and one strand of midivariant rna (mdv-1 rna) template were prepared by incubating the replicase with an excess of mdv-1 (-) rna. in the presence of excess minus strands, these monoenzyme replication complexes were shown to synthesize essentially pure mdv-1 (+) rna in both the first and second cycles of replication. when an equivalent concentration of mutant mdv-1 (-) rna was added to this reaction before completion of the fi ...1979107965
the immobilization response in rhesus monkey: a behavioral and electroencephalographic study. 1979108127
fascioloides magna: development in selected nonruminant mammalian hosts. 1979108129
characteristics of immunoglobulin synthesized early in the development of xenopus laevis. 1979108156
the response to lipopolysaccharide of mouse spleen lymphocytes fractionated on the basis of surface immunoglobulin and complement receptor using fluorescence-activated cell sorting and rosetting techniques. 1979108210
expression of human hepatic genes in mouse hepatoma--human amniocyte hybrids.human liver-specific gene products are expressed by hybrid cells resulting from the fusion of human amniocytes with mouse hepatoma cells. amniocytes grown from human amniotic fluid have no detectable levels of secreted human albumin, transferrin, alpha-1 antitrypsin, or ceruloplasmin, while the mouse hepatoma line, hh--, secretes several mouse liver-specific gene products including transferrin and albumin. fifty-five hybrids were isolated and analyzed for the expression of serum proteins by ouch ...1979107601
frequency and fate of human renal allografts contaminated prior to transplantation.bacterial cultures were done from donor ureter and/or graft perfusion fluid on 92 of 112 patients who had renal transplantations during 1977. three samples of perfusion fluid and 14 samples of donor ureter contained bacteria. in nine cases there was a gram-positive organism, and in 10 cases a gram-negative rod was (among) the contaminant(s). there was no difference in the rate of graft survival, patient survival, or in the frequency of major postoperative complications between the two groups. on ...1979107605
effect of rabbit anti-human thymocyte globulin on lymphocyte subpopulations and functions following allotransplantation in the rhesus monkey.the survival time of skin allografts from rhla-nonidentical, unrelated donors was increased from a mean of 7.69 days in controls (n = 20) to a mean of 32.53 days in rhesus monkeys (n = 21) receiving a total dose of 250 mg of rabbit anti-human thymocyte globulin (ratg) per kg. immunological monitoring studies were performed on the peripheral blood of mononuclear cells in control and treated monkeys. after administration of ratg, the percentage of e rosette-forming cells (e-rfc) was greater than 9 ...1979107631
the viability of cryopreserved aegyptianella pullorum carpano, 1928 in the vector argas (persicargas) walkerae kaiser and hoogstraal, 1969. 1979107668
glyconamides as inhibitors of human beta-glucosidases and beta-galactosidases. 1979107857
effect of 1,25-dihydroxyvitamin d3 on intestinal calcium absorption in strontium-fed rats. 1979107861
spinal cord evaluation by cortical evoked responses.in ten monkeys, selective segmental lesions of the dorsal columns at the upper thoracic and middle cervical levels resulted in almost total attenuation of the cortical evoked potential responses to peripheral nerve stimulation. conversely isolated segmental dorsal column preservation showed intact transmission of the evoked responses at rostral spinal cord, nucleus ventralis posterior lateralis, and cortical levels. responses recorded from the intralaminar thalamic nuclei in the region of nucleu ...1979107931
[correlations of the level of plasmin system and blood serum hyaluronidase activity with pathology of the large intestine in dysentery]. 1979156400
circular dichroism and ultraviolet absorbance of calf thymus dna in presence of ch3hgoh. 1979156470
patterns of neuromuscular disease. as related to stages of normal embryogenesis in voluntary muscle.skeletal muscle samples from the upper and lower extremities of 38 human fetuses (6 to 18 weeks' gestation) of both sexes were studied by histologic, histochemical, and electron microscopic methods. ultrastructural morphometry was applied. in the different stages of normal development are found regressive changes, ranging from reversible dystrophic to irreversible necrotic alterations, which are characteristic of either primary myogenic myopathies or primary neurogenic muscle atrophies in older ...1979156504
the influence of the gamma system on cross-correlated activity of ia muscle spindles and its relation to information transmission.in the decerebrate cat during static or slowly varying (less than 0.3 hz) muscle stretches, the activity of muscle spindle (ms) pairs was poorly correlated and such correlation was not changed after ventral root sectioning. with slightly faster sinusoidal stretches (0.3-20 hz) activity in pairs of ms was also poorly correlated. however, sectioning of the ventral roots produced preferential firing frequencies in the same muscle spindle pairs and increased their degree of correlation. the increase ...1979156885
[activity of the plasmin enzymatic system and of serum hyaluronidase in dysentery]. 1979159382
detection of memory cells by chemical modification of the lymphocyte cell surface.stringent alloantigen requirements, necessary for the differentiation of human memory cells into specific secondary cytolytic t cells (2 degrees ctl), can be bypassed by chemical modification of memory cells with the mitogenic oxidising agent, galactose oxidase. treatment of memory cells generated in a long-term primary mixed lymphocyte culture with neuraminidase and galactose oxidase (nago) results in the differentiation of memory cells into 2 degrees ctl. in contrast, treatment of unprimed cel ...1979162003
muscle spindles in the human anterior digastric muscle.the occurrence of muscle spindles in the anterior digastric muscle in man was investigated and the fibre calibre spectrum of the corresponding nerve was determined. after removal at autopsy from five individuals of both sexes (aged 23--73), the muscles were stained with weigert's iron hematoxylin-van gieson stain and the nerves according to the alzheimer - mann - häggqvist method. altogether 12 spindles were found in five out of ten muscles. only muscles from one individual were devoid of spindl ...1979162026
[blood transfusion and viral diseases. recent acquisitions concerning viral hepatitis viruses, cytomegaloviruses and epstein-barr virus].in recent years, an increasingly clear picture has been formed of the virus-induced syndromes that may follow a blood transfusion or the use of blood derivatives. up to about 10 years ago, post-infusion infection was predominantly due to serum hepatitis. blumberg's discovery of hbsag (formerly known as australia antigen) has made it possible to check and prevent viral hepatitis, type b, and to recognise such distinct forms as the mononucleosis-like syndrome caused by cytomegalic virus, infectiou ...1979219399
electrogenic events in the ubiquinone-cytochrome b/c2 oxidoreductase of rhodopseudomonas sphaeroides.the reductant of ferricytochrome c2 in rhodopseudomonas sphaeroides is a component, z, which has an equilibrium oxidation-reduction reaction involving two electrons and two protons with a midpoint potential of 155 mv at ph 7. under energy coupled conditions, the reduction of ferricytochrome c2 by zh2 is obligatorily coupled to an apparently electrogenic reaction which is monitored by a red shift of the endogeneous carotenoids. both ferricytochrome c2 reduction and the associated carotenoid bands ...1979216398
transcription of the marek's disease virus genome in a nonproductive chicken lymphoblastoid cell line. 1979219594
shigella infection of henle intestinal epithelial cells: role of the host cell.the process of henle 407 embryonic intestinal epithelial cell infection by shigella flexneri 2a m42-43 was studied in an in vitro model system. the role of the henle cell was assessed. it was established that entry of s. flexneri into cells was suppressed by reagents which inhibit uptake of particles by phagocytic cells. the compounds tested included cytochalasin b, dibutyryl-cyclic adenosine monophosphate, choleragen (vibrio cholera enterotoxin), iodoacetate, and dinitrophenol. cytochalasin b i ...1979223988
ornithine decarboxylase activity, cell proliferation, and tumor promotion in mouse epidermis in vivo.the effect of different phorbol esters and of mechanical treatment on the activity of ornithine decarboxylase in mouse epidermis in vivo was investigated. the strong promoter 12-o-tetradecanoylphorbol-13-acetate as well as the weak promoters phorbol dibenzoate and the 12-o-tetradecanoylphorbol-13-acetate analog 12-o-tetradeca-2-cis, 4-trans-6,8-tetraenoylphorobol-13-acetate strongly increased the activity of the enzyme and the intraepidermal level of putrescine, with a maximum at 5 hr after appl ...1979225017
isolation of foot-and-mouth disease virus messenger rna from membrane-bound polyribosomes and characterization of its 5' and 3' termini. 1979228483
[effect of furazolidone on the cyclic nucleotide level in the blood plasma in acute dysentery]. 1979229573
adenosine and deoxyadenosine kinase from rat liver. 1979232628
isolation of the lymphocytosis promoting factor-haemagglutinin of bordetella pertussis by affinity chromatography.the lymphocytosis promoting factor-haemagglutinin of bordetella pertussis was isolated from solutions obtained after cell disintegration by a novel affinity chromatographic method using an adsorbent composed of human haptoglobin covalently attached to a sepharose 4b matrix. the haemagglutinin was bound to the adsorbent at ph 6.5 and eluted by a stepwise change to a ph 10 buffer. a 300--600-fold purification of the haemagglutinin was achieved by this one-step process. the chemical and biological ...1979232665
vomiting and shock in advanced pregnancy (a case report). 1979257956
regional anesthesia in cattle. 1979260529
changes in rat lung produced by olivine dust inhalation. 1979262291
dextro-phenylalanine as inhibitor of the noradrenaline release from the isolated rat hypothalamus.in slices of isolated rat hypothalamus, the dextrophenylalanine (d-phe) 3x10(-3)m produced an inhibition of a 47% in the fractional release of 3h-na to the preparations of holtzman rats, and of 39% to the wistar rats, when the concentration of d-phe (in this last) was of 1x10(-2)m, pointing that the effectiveness of the d-phe is variable with the employed rat root. the mentioned effect is calcium dependent, for it was antagonized when the ca++ was incremented in the medium of 1,68mm (normal) to ...1979262354
biologic properties of lyophilized live typhoid vaccine strains. 1979262514
survival of haemophilus equigenitalis in different antibiotic-containing semen extenders.the ability of haemophilus equigenitalis, the causal agent of contagious equine metritis 1977, to survive in various antibiotic-containing semen extenders was studied at different environmental temperatures. gentamicin sulphate was found to be markedly superior to ampicillin or a combination of sodium benzyl penicillin and polymyxin b sulphate, semen treated with the former antibiotic was either sterile at cultural examination or else yielded appreciably fewer colonies of h. equigenitalis than t ...1979289812
significance of lipid peroxidation in the genesis of chronic vasospasm following rupture of an intracranial aneurysm. 1979290253
the afferent fibres of the sympathetic nervous system. 1979290263
the proportional volume of mucous acinar cells in normal human submandibular salivary glands. 1979294238
[vaccination of ducklings against pasteurellosis and viral hepatitis]. 1979313121
acute epiglottitis: 26 years' experience at the montreal children's hospital.two hundred and four cases of acute epiglottitis treated at the montreal children's hospital between 1951 and 1978 are reviewed. the comparison of tracheostomy vs. nasotracheal intubation is discussed. nasotracheal intubation has been found to be the treatment of choice as long as the necessary services are available in the hospital.1979313456
macrophage fatty acid composition and phagocytosis: effect of unsaturation on cellular phagocytic activity.in order to manipulate the physical properties of the macrophages membrane, methods were developed which potentiated the incorporation of exogenously supplied fatty acids into membrane lipids. chromatograms of macrophages which were grown in the presence of a variety of fatty acids demonstrated that exogenously supplied unsaturated fatty acids (palmitoleic, oleic, elaidic, linoleic, linolenic and arachidonic acids) were readily incorporated into the cells and selectively altered the fatty acyl c ...1979374248
protein and ribonucleic acid syntheses in heat-damaged and heat-killed escherichia coli.protein and ribonucleic acid (rna) syntheses were measured in both lethally injured and thermally damaged viable cells after heating at lethal temperatures. immediately after heating, cells were incubated in growth media containing either [14c]leucine or [3h]uracil. the labeled cells were subsequently treated with penicillin. viable cells were shown to lyse, and the intact nonviable cells were collected by centrifugation. the results showed that after heating, protein and rna synthesis were rein ...1979374390
non-random distribution of transduction termini in transductants from the integrated r plasmid, r100-1.tra+ and tra- derivatives of drug resistance plasmid, r100-1, were isolated by phage p1 from an hfr donor with integrated r100-1 and then analyzed by complementation tests with tra- point mutants of flac. tra+ derivatives of r100-1 carrying tetracycline resistance alone and those carrying all six drug-resistrance genes could support transfer of tra- point mutants of flac except flac traj, whereas all of tra- derivatives of r100-1 failed to complement any one of tra- point mutants of flac. this s ...1979375027
shigella vaginitis: report of 38 patients and review of the literature.thirty-eight cases of shigella vaginitis were identified in a retrospective review of records kept over the past 14 years. the cases of vaginitis were due to three subgroups of shigella. vaginitis varied in severity and duration, persisting for several months in some instances. in 47% of the cases, there was associated bloody vaginal discharge. only two children had diarrhea temporally associated with vaginitis, but six others had had diarrhea. many treatment modalities were used. systemic antib ...1979375177
nucleotide sequence of the transposable dna-element is2.the complete sequence of the transposable dna element is2 in gal op-308:: is2 (i) has been determined. this element is 1.327 bp long. the integrated element is flanked by a five base pair long sequence duplication. the termini of is2 are not perfect inverted repeats, but a close approximation.1979375194
bacteremia caused by shigella flexneri. 1979375454
[conjugative r plasmids of enterobacteria]. 1979375629
[interrelationship between the serum lysozyme level and the leukocyte count in sonne and flexner dysenteries].interrelation between the level of the serum lysozyme and the count of the leucocytes, as well as the severity of the disease was found in testing of the peripheral blood of 133 patients with acute sonnei and flexner dysentery. it is recommended to use the lysozyme index in the clinical practice as one of the additional criteria for estimation of the severity of acute dysentery.1979375827
shigella serotypes in recent isolations at lucknow. 1979376439
further studies on the activation of lymphocytes by endotoxin protein.endotoxin protein, an outer membrane protein of gram-negative bacteria associated with lipopolysaccharide endotoxin, has been found to be a potent activator of lymphocytes. in the absence of t lymphocytes and macrophages, endotoxin protein can stimulate murine b lymphocytes to synthesize dna and produce antibodies of diverse specificities. this stimulation is greater than that obtained with two well known murine b cell activators, lipopolysaccharide endotoxin or ppd-tuberculin. splenic lymphocyt ...1979376715
quantitative determination of titers of anti-zona serum.the titers of rabbit antiserum against isolated mouse zonae pellucidae were examined by several methods in connection with inhibitory effect on fertilization. the titers determined by zona precipitate, zona dissolution, indirect immunofluorescence and in vitro fertilization tests were 2(4), 2(1) to 2(4), 2(7) and 2(4), respectively. cytotoxic effect could not be detected from zona antibody. the indirect immunofluorescence was most sensitive for detection of zona antibody but did not represent th ...1979376778
composition of isolated edema fluid in cold-induced brain edema.edema fluid isolated from cats with cold-induced brain edema was subjected to analysis of electrolyte content, enzyme activities, colloid osmotic pressure and the radioactivity of intravenously injected 99mtc-labeled albumin. the findings corroborate the essential features of vasogenic edema, such as its origin from the blood plasma, its rapid propagation into the white matter of the brain as contrasted with the delayed spread into gray matter, and its contribution to composition of cerebrospina ...1979376787
[storage of shigella flexneri with virulence preserved]. 1979376977
[parenteral use of a therapeutic vaccine in acute dysentery]. 1979377515
[analysis of merodiploid strains of sh. flexneri that have lost virulence].episome f'13 introduced into the genome of a virulent sh. flexneri strain brought about changes in a number of properties of the recipient strain. the expression of these properties was not connected with the chromosome area allelic to the plasmid genome. these changes seem to be induced by the mobilization of the chromosome genes of e. coli. the loss of virulence in sh. flexneri strains carrying episome f'13 seemed to be the consequence of two reasons: the overlapping of kcpa gene by its domina ...1979377868
[search for host specificity systems in shigella using dd-series phages].a total of 712 shigella strains were studied with the use of dysentery diagnostic phages dd ii, dd vi and dd vii in order to reveal the systems of host dna specificity. the study comprised 4 tests: mass screening by the intensity of phagolytic reaction of phages in various strains; and the determination of the parameters of adsorption. as a result, an effective modification and restriction systems were revealed in sh. sonnei 311 with the use of phage dd ii. bacteriophage dd vii was effectively r ...1979377869
[dynamics of the change in sensitivity of shigellae to gentamycin and cephaloridine in vitro].the dynamics of the changes in the shigella sensitivity to gentamicin and cephaloridin was studied in vitro using liquid nutrient media with gradually increasing concentrations of the drugs. 50 passages were performed. it was found that shigella flexner and sonnei decreased their sensitivity to gentamicin to a little extent and remained middle sensitive. sensitivity of shigella flexner to cephaloridin also changed to a little extent, while shigella sonnei became moderately resistant.1979378113
[level and nature of the drug resistance of shigella flexneri].characteristics of antibiotic resistance of 300 strains of shigella flexner 2a isolated from patients within 1976--1977 in the regions where these bacteria were very rare for a long period of time were studied. it was shown that most of the isolates were resistant to chloramphenicol (88.1 per cent), tetracycline (94.8 per cent), streptomycin (89.1 per cent), polymyxin m (82.4 per cent) and others. 46.5--61.6 per cent of the isolates were resistant to neomycin antibiotics. a high level of the res ...1979378114
vivo clearance of enteric bacteria from the hemolymph of the hard clam and the american oyster.american oysters, crassostrea virginica, and hard clams, mercenaria mercenaria, were experimentally contaminated with escherichia coli, salmonella typhimurium, and shigella flexneri either by intracardial injection or via the natural route of ingestion. bacterial inactivation in the hemolymph was monitored for 72 h after exposure to these enteric pathogens at 20 and 6 degrees c. at 6 degrees c, both mean bacterial uptake by ingestion and subsequent clearance was singificantly lower that at 20 de ...1979378127
infectious mononucleosis associated with fatal beta hemolytic streptococcal infection. 1979378507
enzyme-linked immunosorbent assay for immunoglobulin g and immunoglobulin a antibodies to shigella flexneri antigens.an enzyme-linked immunosorbent assay has been developed to detect class-specific antibodies to shigella flexneri lipopolysaccharide antigens. this enzyme-linked immunosorbent assay system has been used to measure antibodies present in serum or intestinal secretions without further purification. it is considerably more sensitive than passive hemagglutination, allowing detection of as little as 1.3 ng of specific immunoglobulin g antibody per ml in immune sera. optimal conditions for this assay ar ...1979378853
molecular characterization of plasmids from virulent and spontaneously occurring avirulent colonial variants of shigella flexneri.spontaneous transition of shigella flexneri from t- to o-type colonies with concomitant loss of virulence dose not appear to be accompanied by a change in any of the four plasmic species found in the virulent parent.1979378861
monocyte function in psoriasis.monocytes derived from the peripheral blood of psoriatic patients demonstrated a significantly higher phagocytic capacity (36 to 40%) for both 125i-labeled shigella flexneri and 125i-labeled staphylococcus albus compared with monocytes from healthy subjects. monocytes from psoriatic patients showed a 2-to-4fold increase in bactericidal capacity against s. albus when compared with normal monocytes. however, the bactericidal capacity of monocytes from diphylline-treated patients did not differ fro ...1979379240
[a dysentery epidemic among tunisia travelers]. 1979379657
[effect of metabolism products of shigella sonnei, sh. flexneri and sh. shigae on proteolytic activity of the organism connective tissue].the activity of proteolytic enzymes, neutral proteinases and cathepsins e+d, was determined in homogenates from the tissues of capsules formed around diffusion chambers with shigella (shigella sonnei, sh. flexneri, sh. shigae) 1, 2, 3, 5 and 10 days after their implantation to the abdominal cavity of rabbits. the specific proteolytic activity in the capsule tissues in the experimental variants is higher in all the studied periods than in the control and its singularities depend on the microbe sp ...1979380094
[sh. flexneri population heterogeneity according to the cellular fatty acid makeup].the composition of fatty acids in the cells of 100 clones of sh. flexneri, strain 9054, isolated from a dysentery patient was studied by the method of gas chromatography. fatty acid composition in 75 clones was similar to the fatty acid composition of the initial culture: 25 clones differed to a variable degree from the prevailing group of clones in the content of unsaturated and cyclopropane fatty acids and, to a considerably lesser extent, in the content of palmitic acid and a number of other ...1979380239
[data to substantiate the early immunological diagnosis of dysentery].the interaction of the whole blood from patients with dysentery and gastrointestinal diseases of non-dysenteric etiology, with the causative agents of dysentery, sh. sonnei and sh. flexneri, and saprophytic, staphylococci labeled with radioactive isotopes was studied in vitro. in dysentery an increase in the capacity of the blood for shigella fixation was observed from the beginning of the disease. during the 1st week of the disease this reaction was strictly specific and accompanied by a decre ...1979380241
[course of dysentery and the specific alteration of the neutrophils in children in the use of monomycin and a diet with an increased protein content].acute dysentery mainly due to shigella sonnei and dynamics of the neutrophil damage index (ndi) of the blood were studied in 80 children at the age of 1 to 14. 20 children (group 1) were treated with monomycin under conditions of a diet with an excessive content of protein (by 25% higher than the physiological norm). 60 children or 3 groups of 20 children each were not subject to such conditions and were considered as control groups. reduction in the time of recovery (by 4 days) and bacteriologi ...1979380456
study on the possible entry of bacterial endotoxin and prostaglandin e2 into the central nervous system from the blood.1 a study has been made of the possible entry of 51cr-bacterial endotoxin and [5,6,8,11,12,14,15(n)-3h]-prostaglandin e2 ([3h5-pge2) into the cns of the anaesthetized cat. 2 no radioactivity was detected in perfusates of the preoptic-anterior hypothalamus or in the cerebrospinal fluid (c.s.f.) in vivo, or in brain tissue post mortem following intracarotid infusion of 51cr-bacterial endotoxin. 3 intracarotid administration of [3h]-pge2 resulted in the entry of radioactivity into the cns of endoto ...1979380709
age-dependent macrophage functions in new zealand black mice. 1979380820
somatic antigens of shigella. the structure of the specific polysaccharide of shigella newcastle (sh. flexneri type 6) lipopolysaccharide.the specific polysaccharide was obtained from the lipopolysaccharide of shigella newcastle by mild acid hydrolysis and further purified by permeation chromatography on sephadex g-50. it was found to consist of l-rhamnose, 2-acetamido-2-deoxy-d-galactose, d-galacturonic acid residues and o-acetyl groups in the molar ratios of 2:1:1:1. on the basis of 1h and 13c nuclear magnetic resonance spectroscopy, methylation analysis, partial acid hydrolysis, smith degradation, and chromium trioxide oxidatio ...1979381001
shigella infection of henle intestinal epithelial cells: role of the bacterium.epithelial cell infection by shigella flexneri 2a was studied in an in vitro model system. using the henle 407 human intestinal epithelial cell line as host cells, a standardized experimental protocol which allowed quantitative measurement of infection was developed. intravellular residence of infecting organisms was confirmed by indirect fluorescent-antibody staining of unfixed and methanol-fixed (henle 407) cells and by quantitative bacteriological culture of disrupted host cells after infecti ...1979381204
[diagnosis of acute appendicitis in patients suffering from diarrhea]. 1979382628
mitomycin c stimulates production of a toxin in shigella species that causes morphological changes in chinese hamster ovary cells.shigella dysenteriae produced a toxin that caused morphological changes in chinese hamster ovary cells which was different from the neurotoxin (y. takeda, k. okamoto, and t. miwatani, infect. immun. 18:546--548, 1977). it was found that the toxin was produced not only by s. dysenteriae but also by s. flexneri, s. boydii, and s. sonnei. the production of the toxin was stimulated by the addition of 1 microgram of mitomycin c per ml to the culture in the early logarithmic growth phase.1979370009
antigenic similarity of morphologic type ciii1 bacteriophages. 1979397728
phagocyte functions in familial mediterranean fever.monocytes derived from peripheral blood of patients with familial mediterranean fever (f.m.f.) demonstrated lower phagocytic capacity for shigella flexneri and depressed bactericidal activity against s. albus when compared to monocytes from healthy individuals. treatment of patients with colchicine did not alter these functions. on the other hand, chemokinesis of pmn of f.m.f. patients was enhanced especially during attacks. colchicine treatment decreased significantly the pmn chemotactic migrat ...1979397753
comparison of some shigella flexneri (escherichia coli) hybrids in cross agglutination and agglutinin absorption tests.antigenic structure of lac+ hybrids selected from escherichia coli x shigella flexneri crosses was compared in the cross agglutination and absorption tests. identity of the hybrids from the recipients of serotype 2a and variant y was proved, as well as from serotypes 1b and 4b. the latter hybrids were coupled with shigella flexneri strains 3a, 3b and 3c. the hybrids appeared almost identical with serotype 3c.1979398206
histoautoradiographic investigation of the invasive dissemination of ehrlich ascites tumor cells through the organs of the mouse.in this paper the attempt has been pursued to quantify by a well planned experimental technique the ability of the neoplastic cells to spread around in vivo through various organs of laboratory animals. by making use of the histoautoradiographic techniques the incorporation of 3h thymidine either by the normal and by the neoplastic cells has been investigated at many different anatomical sites in ehrlich ascites tumor-bearing mice. the method has been shown to be well suited also for the quantit ...1979398216
the intestinal flora and infant botulism.the intestinal flora of experimental animals interferes with infection by species of salmonella and shigella. protection against infection with these organisms appears to be related to high concentrations of volatile acids, low ph, and low oxidation-reduction potential of the intestinal contents of animals with an intact flora. there are no data to show that the flora influences colonization of the intestine with clostridial species, but indirect evidence suggests that the intestinal flora may b ...1979399374
colonization by salmonella typhimurium and shigella flexneri iii of the gastrointestinal tract of mice treated with beta-2-thienylalanine and streptomycin.mice fed beta-2-thienylalanine (beta-2-t) by oesophageal tube were no more susceptible to gastrointestinal tract colonization by salmonella typhimurium or shigella flexneri iii than control mice fed water. in both beta-2-t-fed and water-fed groups, the increasing dosage of s. typhimurium, in logarithmic increments to groups of mice, resulted in increasing numbers of these bacteria detectable on dilution plates from organ homogenates. colonization by s. flexneri iii only occurred at a dosage of 1 ...1979399720
[research methods of r factors (author's transl)].after having outlined the routine methods for the study of drug resistance plasmids used at highly specialized centres such as the london enteric reference laboratory, the aa. examine the most practical methods of a pure and simple way of discovering r factors in enterobacteria. a brief reference is made to some of the in vivo transfer techniques before reducing the various possibilities of in vitro survey to essentials. the experimental conditions realized by some aa. for mating in liquid media ...1979400251
[drug resistance of shigella strains isolated in central italy during 1969-1976 (author's transl)].only ninety-three strains of shigella, eightyseven of which have been identified as s. sonnei and six as s. flexneri were collected during 1969-1976 at the pathogen enterobacteria centre of pisa in central italy, as evidence of an epidemiological situation marked by a rare occurrence of syndromes related to these organisms. the collected strains were tested with disks of nine antibacterial drugs in common use: the survey detected sensitivity of all the strains to cephaloridine, ampicillin, carbe ...1979400254
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