Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| modes and modulations of antibiotic resistance gene expression. | since antibiotic resistance usually affords a gain of function, there is an associated biological cost resulting in a loss of fitness of the bacterial host. considering that antibiotic resistance is most often only transiently advantageous to bacteria, an efficient and elegant way for them to escape the lethal action of drugs is the alteration of resistance gene expression. it appears that expression of bacterial resistance to antibiotics is frequently regulated, which indicates that modulation ... | 2007 | 17223624 |
| the role of carbapenems in initial therapy for serious gram-negative infections. | the treatment of patients with serious gram-negative infections must be both prompt and correct. numerous studies have demonstrated that mortality risk is significantly increased when the initial antibiotic regimen does not adequately cover the infecting pathogen. furthermore, changing to an appropriate regimen once culture results are available does not reduce this risk. therefore, one must empirically treat serious infections with a regimen that covers likely pathogens. selecting such a regime ... | 2008 | 18495062 |
| detection of methyltransferases conferring high-level resistance to aminoglycosides in enterobacteriaceae from europe, north america, and latin america. | the alteration of ribosomal targets by recently described 16s rrna methyltransferases confers resistance to most aminoglycosides, including arbekacin. enterobacteriaceae and nonfermentative bacilli acquired through global surveillance programs were screened for the presence of these enzymes on the basis of phenotypes that were resistant to nine tested aminoglycosides. subsequent molecular studies determined that 20 of 21 (95.2%) methyltransferase-positive isolates consisted of novel species reco ... | 2008 | 18347105 |
| a conservative region of the mercuric reductase gene (mera) as a molecular marker of bacterial mercury resistance. | the most common bacterial mercury resistance mechanism is based on the reduction of hg(ii) to hg(0), which is dependent of the mercuric reductase enzyme (mera) activity. the use of a 431 bp fragment of a conservative region of the mercuric reductase (mera) gene was applied as a molecular marker of this mechanism, allowing the identification of mercury resistant bacterial strains. | 2008 | 24031221 |
| in vitro activity of lk-157, a novel tricyclic carbapenem as broad-spectrum {beta}-lactamase inhibitor. | lk-157 is a novel tricyclic carbapenem with potent activity against class a and class c beta-lactamases. when tested against the purified tem-1 and shv-1 enzymes, lk-157 exhibited 50% inhibitory concentrations (ic(50)s) in the ranges of the clavulanic acid and tazobactam ic(50)s (55 nm and 151 nm, respectively). moreover, lk-157 significantly inhibited ampc beta-lactamase (ic(50), 62 nm), as lk-157 was >2,000-fold more potent than clavulanic acid and approximately 28-fold more active than tazoba ... | 2008 | 19075067 |
| in vitro activity of lk-157, a novel tricyclic carbapenem as broad-spectrum {beta}-lactamase inhibitor. | lk-157 is a novel tricyclic carbapenem with potent activity against class a and class c beta-lactamases. when tested against the purified tem-1 and shv-1 enzymes, lk-157 exhibited 50% inhibitory concentrations (ic(50)s) in the ranges of the clavulanic acid and tazobactam ic(50)s (55 nm and 151 nm, respectively). moreover, lk-157 significantly inhibited ampc beta-lactamase (ic(50), 62 nm), as lk-157 was >2,000-fold more potent than clavulanic acid and approximately 28-fold more active than tazoba ... | 2008 | 19075067 |
| establishment of in vitro susceptibility testing methodologies and comparative activities of piperacillin in combination with the penem {beta}-lactamase inhibitor bli-489. | the novel bicyclic penem inhibitor bli-489 has demonstrated activity as an inhibitor of class a, c, and d beta-lactamases. to determine the combination of piperacillin and bli-489 to be used in susceptibility testing that would most accurately identify susceptible and resistant isolates, a predictor panel of beta-lactamase-producing bacteria was utilized to determine the reliability of the combination of piperacillin-bli-489 at a constant inhibitor concentration of 2 or 4 microg/ml and at ratios ... | 2008 | 19001109 |
| establishment of in vitro susceptibility testing methodologies and comparative activities of piperacillin in combination with the penem {beta}-lactamase inhibitor bli-489. | the novel bicyclic penem inhibitor bli-489 has demonstrated activity as an inhibitor of class a, c, and d beta-lactamases. to determine the combination of piperacillin and bli-489 to be used in susceptibility testing that would most accurately identify susceptible and resistant isolates, a predictor panel of beta-lactamase-producing bacteria was utilized to determine the reliability of the combination of piperacillin-bli-489 at a constant inhibitor concentration of 2 or 4 microg/ml and at ratios ... | 2008 | 19001109 |
| evaluation of quinolone resistance in gram negative bacilli isolated from community- and hospital-acquired infections. | gram negative bacilli are among the most important microbial agents involved in both hospital- and community-acquired infections. the quinolones are preferred antibacterial agents for the treatment of both community- and hospital-acquired urinary tract infections caused by gram negative bacilli because of their strong antibacterial effects, and because they can be administered both orally and parenterally. in this study, it was aimed to determine the sensitivity of gram negative bacteria isolate ... | 2008 | 25610028 |
| in vitro antibacterial activity of ce-156811, a novel analog derived from hygromycin a. | we evaluated a novel truncated hygromycin a analog in which the furanose ring was replaced with a 2-fluoro-2-cyclopropylethyl substituent for its activity against multidrug resistant gram-positive bacteria and compared its activity to the activities of linezolid, quinupristin-dalfopristin, and vancomycin. ce-156811 demonstrated robust in vitro activity against gram-positive bacteria that was comparable to that of linezolid. | 2008 | 18426902 |
| the dual role of bacteriocins as anti- and probiotics. | bacteria employed in probiotic applications help to maintain or restore a host's natural microbial floral. the ability of probiotic bacteria to successfully outcompete undesired species is often due to, or enhanced by, the production of potent antimicrobial toxins. the most commonly encountered of these are bacteriocins, a large and functionally diverse family of antimicrobials found in all major lineages of bacteria. recent studies reveal that these proteinaceous toxins play a critical role in ... | 2008 | 18853155 |
| presence of the kpc carbapenemase gene in enterobacteriaceae causing bacteremia and its correlation with in vitro carbapenem susceptibility. | during 6 months, we obtained enterobacteriaceae isolates from patients with gram-negative bacteremia at a 1,250-bed teaching hospital in st. louis, mo, and compared carbapenem susceptibilities with the presence of bla(kpc), a transferable carbapenemase gene. three (1.2%) out of 243 isolates were bla(kpc) positive. ertapenem nonsusceptibility had a low positive predictive value. | 2008 | 19020072 |
| presence of the kpc carbapenemase gene in enterobacteriaceae causing bacteremia and its correlation with in vitro carbapenem susceptibility. | during 6 months, we obtained enterobacteriaceae isolates from patients with gram-negative bacteremia at a 1,250-bed teaching hospital in st. louis, mo, and compared carbapenem susceptibilities with the presence of bla(kpc), a transferable carbapenemase gene. three (1.2%) out of 243 isolates were bla(kpc) positive. ertapenem nonsusceptibility had a low positive predictive value. | 2008 | 19020072 |
| high prevalence of the aac(6')-ib-cr gene and its dissemination among enterobacteriaceae isolates by ctx-m-15 plasmids in bulgaria. | 2008 | 19001110 | |
| high prevalence of the aac(6')-ib-cr gene and its dissemination among enterobacteriaceae isolates by ctx-m-15 plasmids in bulgaria. | 2008 | 19001110 | |
| brain endothelial cell-cell junctions: how to "open" the blood brain barrier. | the blood-brain barrier (bbb) is a highly specialized structural and biochemical barrier that regulates the entry of blood-borne molecules into brain, and preserves ionic homeostasis within the brain microenvironment. bbb properties are primarily determined by junctional complexes between the cerebral endothelial cells. these complexes are comprised of tight and adherens junctions. such restrictive angioarchitecture at the bbb reduces paracellular diffusion, while minimal vesicle transport activ ... | 2008 | 19506719 |
| daily cranberry juice for the prevention of asymptomatic bacteriuria in pregnancy: a randomized, controlled pilot study. | we compared the effects of daily cranberry juice cocktail to those of placebo during pregnancy on asymptomatic bacteriuria and symptomatic urinary tract infections. | 2008 | 18707726 |
| dynamics of the mucosa-associated flora in ulcerative colitis patients during remission and clinical relapse. | the colonic mucosa-associated flora (maf) in patients with active ulcerative colitis (uc) (n = 13) was investigated by examining 16s rrna gene signatures during remission and relapse against levels for controls (n = 5). baseline reduction, temporal instability, and decrease of bacterial richness toward relapse were observed for uc patients, whereas the maf for controls was stable over time. | 2008 | 18701655 |
| enzyme structural plasticity and the emergence of broad-spectrum antibiotic resistance. | the emergence of multi-resistant pathogenic bacteria is a worldwide health issue. recently, clinical variants of a single antibiotic-modifying acetyltransferase, aac(6')-ib-a variant of aminoglycoside 6'-n-acetyltransferase-have been identified that confer extended resistance to most aminoglycosides and, more surprisingly, to structurally unrelated fluoroquinolones. the corresponding gene is carried by mobile genetic elements and is present in most multi-resistant pathogenic strains, hence makin ... | 2008 | 18292754 |
| sexual intercourse and risk of symptomatic urinary tract infection in post-menopausal women. | sexual intercourse increases the risk of symptomatic urinary tract infections (uti) in young women, but its role among post-menopausal women is unclear. | 2008 | 18266044 |
| comparison of alad activities of citrobacter and pseudomonas strains and their usage as biomarker for pb contamination. | delta-aminolevulinate dehydratase (alad) activity has been used in prokaryotes and eukaryotes as a biomarker for environmental lead (pb) exposure and toxicity. microorganisms are sensitive indicators of toxicity at the fundamental level of ecological organization, but bacterial biomarker studies are focused on the pseudomonas strains in group i and e coli. the objectives of the present work were to determine if burkholderia gladioli belonging to group ii, due to its 16srrna similarity, can be us ... | 2008 | 17516138 |
| biohydrogen production by enterobacter cloacae and citrobacter freundii in carrier induced granules. | carrier induced granular particles comprising enterobacter cloacae and citrobacter freundii were used to generate h(2) from sucrose in an anaerobic fluidized bed bioreactor. at a hydraulic retention time of 4.5 h, 95.8% of the sucrose was consumed and the rate of h(2) production reached 180 mmol h(2) l h(-1). biogas composition for h(2) and co(2) was 42 and 55%, respectively. | 2008 | 17876534 |
| molecular epidemiology of clinically significant antibiotic resistance genes. | antimicrobials were first introduced into medical practice a little over 60 years ago and since that time resistant strains of bacteria have arisen in response to the selective pressure of their use. this review uses the paradigm of the evolution and spread of beta-lactamases and in particular beta-lactamases active against antimicrobials used to treat gram-negative infections. the emergence and evolution particularly of ctx-m extended-spectrum beta-lactamases (esbls) is described together with ... | 2008 | 18311156 |
| comparative activity of meropenem in us medical centers (2007): initiating the 2nd decade of mystic program surveillance. | since 1997, the meropenem yearly susceptibility test information collection (mystic) program has monitored the antimicrobial activity of broad-spectrum agents against pathogens from hospitalized patients. in the united states, 2894 isolates were submitted in 2007 from 15 sites, including 1392 enterobacteriaceae, 643 nonfermentative gram-negative bacilli, and 829 gram-positive cocci. all isolates were tested by broth microdilution methods. meropenem (mic(90) range, 0.12-2 microg/ml) exhibited the ... | 2008 | 18329835 |
| distribution of tetracycline and streptomycin resistance genes and class 1 integrons in enterobacteriaceae isolated from dairy and nondairy farm soils. | the prevalence of selected tetracycline and streptomycin resistance genes and class 1 integrons in enterobacteriaceae (n = 80) isolated from dairy farm soil and nondairy soils was evaluated. among 56 bacteria isolated from dairy farm soils, 36 (64.3%) were resistant to tetracycline, and 17 (30.4%) were resistant to streptomycin. lower frequencies of tetracycline (9 of 24 or 37.5%) and streptomycin (1 of 24 or 4.2%) resistance were observed in bacteria isolated from nondairy soils. bacteria (n = ... | 2008 | 17701242 |
| antimicrobial activity of the crude extracts and five flavonoids from the twigs of dorstenia barteri (moraceae). | the aim of this study was to evaluate the antimicrobial activity of the crude extract of the twigs of dorstenia barteri (dbt) as well as that of four of the five flavonoids isolated from this extract. gram-positive bacteria (six species), gram-negative bacteria (12 species) and fungi (four species) were used. the agar disc diffusion test was used to determine the sensitivity of the tested samples while the well micro-dilution was used to determine the minimal inhibition concentrations (mic) and ... | 2008 | 18280679 |
| antimicrobial-resistant pathogens in intensive care units in canada: results of the canadian national intensive care unit (can-icu) study, 2005-2006. | between 1 september 2005 and 30 june 2006, 19 medical centers collected 4,180 isolates recovered from clinical specimens from patients in intensive care units (icus) in canada. the 4,180 isolates were collected from 2,292 respiratory specimens (54.8%), 738 blood specimens (17.7%), 581 wound/tissue specimens (13.9%), and 569 urinary specimens (13.6%). the 10 most common organisms isolated from 79.5% of all clinical specimens were methicillin-susceptible staphylococcus aureus (mssa) (16.4%), esche ... | 2008 | 18285482 |
| high-level carbapenem resistance in a citrobacter freundii clinical isolate is due to a combination of kpc-2 production and decreased porin expression. | an imipenem-resistant isolate of citrobacter freundii zj163 (mic 256 microg ml(-1)) isolated from a chinese hospital was investigated. the c. freundii zj163 isolate exhibited high-level resistance to carbapenems, penicillins, cephalosporins, cefoxitin, aztreonam, quinolones and aminoglycosides. isoelectric focusing (ief) demonstrated three beta-lactamases with pis of 5.4 (tem-1), 6.7 (kpc-2) and 7.9 (ctx-m-14). two different transconjugants (types a and b) were obtained by conjugation studies. t ... | 2008 | 18287296 |
| citrobacter freundii infection in glutaric aciduria type 1: adding insult to injury. | glutaric aciduria type 1 (ga1) is an inborn error of organic acid metabolism, where the brain is the principal organ affected with exposure to toxic metabolic product, 3-hydroxyglutaric acid (3-ohga). a 2-year-old boy with ga1 and delayed developmental milestones had an acute neurological crisis leading to massive brain abscess with citrobacter freundi infection, a rare cause of neonatal meningitis and often associated with brain abscess. both 3-ohga and c. freundii can damage the blood-brain ba ... | 2008 | 18296804 |
| detection of the klebsiella pneumoniae carbapenemase type 2 carbapenem-hydrolyzing enzyme in clinical isolates of citrobacter freundii and k. oxytoca carrying a common plasmid. | the klebsiella pneumoniae carbapenemase (kpc) was detected in carbapenem-resistant isolates of citrobacter freundii and klebsiella oxytoca recovered from different patients in a michigan hospital. restriction analysis and hybridization with a kpc-specific probe showed the bla(kpc-2) genes of these two genera of the family enterobacteriaceae are carried on a common plasmid. | 2008 | 18385437 |
| mutations in ampg or ampd affect peptidoglycan fragment release from neisseria gonorrhoeae. | neisseria gonorrhoeae releases peptidoglycan fragments during growth. the majority of the fragments released are peptidoglycan monomers, molecules known to increase pathogenesis through the induction of proinflammatory cytokines and responsible for the killing of ciliated epithelial cells. in other gram-negative bacteria such as escherichia coli, these peptidoglycan fragments are efficiently degraded and recycled. peptidoglycan fragments enter the cytoplasm from the periplasm via the ampg permea ... | 2008 | 18390650 |
| krüppel-like factor 5 mediates transmissible murine colonic hyperplasia caused by citrobacter rodentium infection. | krüppel-like factor 5 (klf5) is a transcription factor that is highly expressed in proliferating crypt cells of the intestinal epithelium. klf5 has a pro-proliferative effect in vitro and is induced by mitogenic and stress stimuli. to determine whether klf5 is involved in mediating proliferative responses to intestinal stressors in vivo, we examined its function in a mouse model of transmissible murine colonic hyperplasia triggered by colonization of the mouse colon by the bacteria citrobacter r ... | 2008 | 18395082 |
| exposure-response analyses of tigecycline efficacy in patients with complicated intra-abdominal infections. | exposure-response analyses were performed to test the microbiological and clinical efficacies of tigecycline in complicated intra-abdominal infections where escherichia coli and bacteroides fragilis are the predominant pathogens. data from evaluable patients enrolled in three clinical trials were pooled. patients received intravenous tigecycline (100-mg loading dose followed by 50 mg every 12 h or 50-mg loading dose followed by 25 mg every 12 h). at the test-of-cure visit, microbiological and cl ... | 2008 | 17954694 |
| virulence studies of enterobacter sakazakii isolates associated with a neonatal intensive care unit outbreak. | in 1994, an outbreak of enterobacter sakazakii infections in france occurred in a neonatal intensive care unit during which 17 neonates were infected. more than half of the infected neonates had severe clinical symptoms; 7 cases of necrotising enterocolitis (one with abdominal perforation), one case of septicemia, and one case of meningitis. the other 8 neonates were shown to be colonized but remained asymptomatic. there were three deaths. four distinguishable pulsotypes of e. sakazakii were iso ... | 2008 | 18423002 |
| boronic acid disk tests for identification of extended-spectrum beta-lactamase production in clinical isolates of enterobacteriaceae producing chromosomal ampc beta-lactamases. | a study using boronic acid (ba) was designed to detect the extended-spectrum beta-lactamases (esbls) in enterobacteriaceae producing chromosomal ampc beta-lactamases. a total of 197 clinical isolates of enterobacter spp. (n=100), serratia marcescens (n=62) and citrobacter freundii (n=35) were analysed. genes encoding esbls were detected by polymerase chain reaction (pcr) amplification followed by direct sequencing of pcr products. the clinical and laboratory standards institute confirmatory test ... | 2008 | 18337065 |
| the effects of bee (apis mellifera) venom phospholipase a2 on trypanosoma brucei brucei and enterobacteria. | the potential role of phospholipases in trypanosomiasis was investigated using bee venom phospholipase a2 (bvpla2) as a model. the effects of bvpla2 on the survival of trypanosoma brucei brucei, 2h and 12h cultures of enterobacter cloacae, escherichia coli, citrobacter freundii were studied. about 1 mg ml(-1) bvpla2 was trypanocidal after 30 min. some growth occurred at lower concentrations up to 2h after treatment but viability decreased up to 8h. even very low concentrations of bvpla2 (10(-12) ... | 2008 | 18343372 |
| gram-negative bacteraemia in non-icu patients: factors associated with inadequate antibiotic therapy and impact on outcomes. | a considerable number of gram-negative bacteraemias occur outside intensive care units (icus). inadequate antibiotic therapy in icus has been associated with adverse outcomes; however, there are no prospective studies in non-icu patients. | 2008 | 18344548 |
| development and evaluation of immunochromatographic assay for simple and rapid detection of campylobacter jejuni and campylobacter coli in human stool specimens. | an immunochromatographic assay (campy-ica) using a newly generated single monoclonal antibody against a 15-kda cell surface protein of campylobacter jejuni was developed. when cell suspensions of 86 c. jejuni strains and 27 campylobacter coli strains were treated with a commercially available bacterial protein extraction reagent and the resulting extracts were tested with the campy-ica, they all yielded positive results. the minimum detectable limits for the c. jejuni strains ranged from 1.8 x 1 ... | 2008 | 18256225 |
| prevalence of fecal carriage of acquired expanded-spectrum cephalosporin resistance in enterobacteriaceae strains from cattle in france. | 2008 | 18272707 | |
| evaluation of different laboratory tests for the detection of metallo-beta-lactamase production in enterobacteriaceae. | clinical isolates of klebsiella pneumoniae (91), escherichia coli (49), enterobacter spp. (27), proteus mirabilis (17), citrobacter freundii (2), providencia stuartii (3) and serratia spp. (5), with various mics of imipenem, were examined for production of metallo-beta-lactamases (mbls) with different phenotypic laboratory tests that have been previously published to detect mbls in pseudomonas aeruginosa and acinetobacter spp. | 2008 | 18222954 |
| campylobacter jejuni survives within epithelial cells by avoiding delivery to lysosomes. | campylobacter jejuni is one of the major causes of infectious diarrhea world-wide, although relatively little is know about its mechanisms of pathogenicity. this bacterium can gain entry into intestinal epithelial cells, which is thought to be important for its ability to persistently infect and cause disease. we found that c. jejuni is able to survive within intestinal epithelial cells. however, recovery of intracellular bacteria required pre-culturing under oxygen-limiting conditions, suggesti ... | 2008 | 18225954 |
| linkage of acquired quinolone resistance (qnrs1) and metallo-beta-lactamase (blavim-1) genes in multiple species of enterobacteriaceae from bolzano, italy. | twenty-four of 209 oxyimino-cephalosporin- and/or aztreonam-resistant enterobacteriaceae collected around bolzano had reduced susceptibility or resistance to carbapenems and gave positive metallo-beta-lactamase (mbl) tests. their resistance mechanisms were investigated. | 2008 | 18184647 |
| distribution of conjugative-plasmid-mediated 16s rrna methylase genes among amikacin-resistant enterobacteriaceae isolates collected in 1995 to 1998 and 2001 to 2006 at a university hospital in south korea and identification of conjugative plasmids mediating dissemination of 16s rrna methylase. | the distribution of conjugative-plasmid-mediated 16s rrna methylase genes among amikacin-resistant enterobacteriaceae collected between 1995 and 1998 and between 2001 and 2006 at a university hospital in south korea was examined, and conjugative plasmids carrying the 16s rrna methylase genes were characterized by pcr-based replicon typing and by determination of their antimicrobial resistance pattern. among the 7,127 isolates, 463 isolates showed a high level of resistance to amikacin, and 218 o ... | 2008 | 18094126 |
| prevalence of ampc and other beta-lactamases in enterobacteria at a large urban university hospital in brazil. | production of extended-spectrum beta-lactamases (esbls) has been reported in virtually all species of enterobacteriaceae, which greatly complicates the therapy for infections caused by these organisms. however, the frequency of isolates producing ampc beta-lactamases, especially plasmid-mediated ampc (pampc), is largely unknown. these beta-lactamases confer resistance to extended-spectrum cephalosporins and aztreonam, a multidrug-resistant (mdr) profile. the aim of the present study was to deter ... | 2008 | 17900845 |
| prevalence of ampc and other beta-lactamases in enterobacteria at a large urban university hospital in brazil. | production of extended-spectrum beta-lactamases (esbls) has been reported in virtually all species of enterobacteriaceae, which greatly complicates the therapy for infections caused by these organisms. however, the frequency of isolates producing ampc beta-lactamases, especially plasmid-mediated ampc (pampc), is largely unknown. these beta-lactamases confer resistance to extended-spectrum cephalosporins and aztreonam, a multidrug-resistant (mdr) profile. the aim of the present study was to deter ... | 2008 | 17900845 |
| modulation of intestinal goblet cell function during infection by an attaching and effacing bacterial pathogen. | the attaching and effacing (a/e) bacterial pathogens enteropathogenic escherichia coli and enterohemorrhagic e. coli and the related mouse pathogen citrobacter rodentium colonize their hosts' intestines by infecting the apical surfaces of enterocytes, subverting their function, and they ultimately cause diarrhea. surprisingly, little is known about the interactions of these organisms with goblet cells, which are specialized epithelial cells that secrete the protective molecules muc2 and trefoil ... | 2008 | 17984203 |
| modulation of intestinal goblet cell function during infection by an attaching and effacing bacterial pathogen. | the attaching and effacing (a/e) bacterial pathogens enteropathogenic escherichia coli and enterohemorrhagic e. coli and the related mouse pathogen citrobacter rodentium colonize their hosts' intestines by infecting the apical surfaces of enterocytes, subverting their function, and they ultimately cause diarrhea. surprisingly, little is known about the interactions of these organisms with goblet cells, which are specialized epithelial cells that secrete the protective molecules muc2 and trefoil ... | 2008 | 17984203 |
| broad-host-range yersinia phage py100: genome sequence, proteome analysis of virions, and dna packaging strategy. | py100 is a lytic bacteriophage with a broad host range within the genus yersinia. the phage forms plaques on strains of the three human pathogenic species yersinia enterocolitica, y. pseudotuberculosis, and y. pestis at 37 degrees c. py100 was isolated from farm manure and intended to be used in phage therapy trials. py100 has an icosahedral capsid containing double-stranded dna and a contractile tail. the genome consists of 50,291 bp and is predicted to contain 93 open reading frames (orfs). py ... | 2008 | 17965162 |
| broad-host-range yersinia phage py100: genome sequence, proteome analysis of virions, and dna packaging strategy. | py100 is a lytic bacteriophage with a broad host range within the genus yersinia. the phage forms plaques on strains of the three human pathogenic species yersinia enterocolitica, y. pseudotuberculosis, and y. pestis at 37 degrees c. py100 was isolated from farm manure and intended to be used in phage therapy trials. py100 has an icosahedral capsid containing double-stranded dna and a contractile tail. the genome consists of 50,291 bp and is predicted to contain 93 open reading frames (orfs). py ... | 2008 | 17965162 |
| primary pyomyositis of the paraspinal muscles: a case report and literature review. | a case of non-tropical pyomyositis in a healthy, adolescent, 13-year-old boy, affecting the paraspinal muscles is presented. computerised axial tomography scan (ct scan) of the spine provided valuable information on the nature, extent of the disease and helped to plan successful surgical management. none of the reported cases of such severity of paraspinal pyomyositis had involvement of quadratus lumborum muscle or compression on retroperitoneal organ as in our case. | 2008 | 17932692 |
| primary pyomyositis of the paraspinal muscles: a case report and literature review. | a case of non-tropical pyomyositis in a healthy, adolescent, 13-year-old boy, affecting the paraspinal muscles is presented. computerised axial tomography scan (ct scan) of the spine provided valuable information on the nature, extent of the disease and helped to plan successful surgical management. none of the reported cases of such severity of paraspinal pyomyositis had involvement of quadratus lumborum muscle or compression on retroperitoneal organ as in our case. | 2008 | 17932692 |
| lack of response of int-407 cells to the presence of non-culturable campylobacter jejuni. | many contradictory articles on the infectivity of non-culturable campylobacter jejuni can be found. we studied the effect of non-culturable c. jejuni in an in vitro assay. to prevent the potential effect of a few culturable bacteria in the non-culturable suspension, int-407 cells, which mimic the outer cell layer in the small intestines, were exposed to culturable c. jejuni suspensions with or without non-culturable c. jejuni. the number of bacteria adhering to and/or invading int-407 cells and ... | 2008 | 18081950 |
| lack of response of int-407 cells to the presence of non-culturable campylobacter jejuni. | many contradictory articles on the infectivity of non-culturable campylobacter jejuni can be found. we studied the effect of non-culturable c. jejuni in an in vitro assay. to prevent the potential effect of a few culturable bacteria in the non-culturable suspension, int-407 cells, which mimic the outer cell layer in the small intestines, were exposed to culturable c. jejuni suspensions with or without non-culturable c. jejuni. the number of bacteria adhering to and/or invading int-407 cells and ... | 2008 | 18081950 |
| novel variant of the qnrb gene, qnrb12, in citrobacter werkmanii. | 2008 | 18086833 | |
| novel variant of the qnrb gene, qnrb12, in citrobacter werkmanii. | 2008 | 18086833 | |
| emergence of antibiotic resistance during therapy for infections caused by enterobacteriaceae producing ampc beta-lactamase: implications for antibiotic use. | enterobacter spp., serratia marcescens, citrobacter freundii, and morganella morganii are characterized by chromosomally encoded ampc beta-lactamases and possess the ability to develop resistance upon exposure to broad-spectrum cephalosporins. to determine the incidences of the emergence of resistance during antimicrobial therapy for infections caused by these organisms and the effect of the emergence of resistance on patient outcomes, all patients who were admitted to the asan medical center (s ... | 2008 | 18086837 |
| isolation and purification of enterocin e-760 with broad antimicrobial activity against gram-positive and gram-negative bacteria. | strain nrrl b-30745, isolated from chicken ceca and identified as enterococcus durans, enterococcus faecium, or enterococcus hirae, was initially identified as antagonistic to campylobacter jejuni. the isolate produced a 5,362-da bacteriocin (enterocin) that inhibits the growth of salmonella enterica serovar enteritidis, s. enterica serovar choleraesuis, s. enterica serovar typhimurium, s. enterica serovar gallinarum, escherichia coli o157:h7, yersinia enterocolitica, citrobacter freundii, klebs ... | 2008 | 18086839 |
| risk of gastrointestinal disease associated with exposure to pathogens in the water of the lower passaic river. | during precipitation events, untreated human sewage is often intentionally discharged to surface water bodies via combined sewer overflow (cso) systems in order to avoid overloading wastewater treatment plants. the purpose of this analysis was to evaluate the risk of pathogen-related disease associated with cso discharges into the lower passaic river. concentrations of fecal coliform, total coliform, fecal streptococcus, and fecal enterococcus bacteria were measured at six river locations on six ... | 2008 | 18156342 |
| risk of gastrointestinal disease associated with exposure to pathogens in the water of the lower passaic river. | during precipitation events, untreated human sewage is often intentionally discharged to surface water bodies via combined sewer overflow (cso) systems in order to avoid overloading wastewater treatment plants. the purpose of this analysis was to evaluate the risk of pathogen-related disease associated with cso discharges into the lower passaic river. concentrations of fecal coliform, total coliform, fecal streptococcus, and fecal enterococcus bacteria were measured at six river locations on six ... | 2008 | 18156342 |
| identification of type 3 fimbriae in uropathogenic escherichia coli reveals a role in biofilm formation. | catheter-associated urinary tract infection (cauti) is the most common nosocomial infection in the united states. uropathogenic escherichia coli (upec), the most common cause of cauti, can form biofilms on indwelling catheters. here, we identify and characterize novel factors that affect biofilm formation by upec strains that cause cauti. sixty-five cauti upec isolates were characterized for phenotypic markers of urovirulence, including agglutination and biofilm formation. one isolate, e. coli m ... | 2008 | 18055599 |
| extended-spectrum +¦-lactamase (esbl) in omani children: study of prevalence, risk factors and clinical outcomes at sultan qaboos university hospital, sultanate of oman. | antimicrobial resistance is a growing problem worldwide, which imposes difficulties in the selection of appropriate empirical antimicrobial therapy. this study evaluated extended-spectrum +¦-lactamase (esbl) isolates in 2005 in the department of child health at sultan qaboos university hospital (squh), oman. | 2008 | 21748055 |
| phlegmonous gastritis after endoscopic submucosal dissection for early gastric cancer. | a 74-year-old man with diabetic nephropathy developed epigastric pain and high fever after endoscopic submucosal dissection (esd) for early gastric cancer. gastroscopy, endoscopic ultrasonography and computed tomography showed ulceration with a purulent lake, thickened entire gastric mucosal layers suggesting focal abscess formation, leading to the diagnosis of phlegmonous gastritis. he underwent total gastrectomy as an emergency. histological findings of the resected specimen showed severe infl ... | 2008 | 21180517 |
| massively parallel pathogen identification using high-density microarrays. | identification of microbial pathogens in clinical specimens is still performed by phenotypic methods that are often slow and cumbersome, despite the availability of more comprehensive genotyping technologies. we present an approach based on whole-genome amplification and resequencing microarrays for unbiased pathogen detection. this 10 h process identifies a broad spectrum of bacterial and viral species and predicts antibiotic resistance and pathogenicity and virulence profiles. we successfully ... | 2008 | 21261824 |
| massively parallel pathogen identification using high-density microarrays. | identification of microbial pathogens in clinical specimens is still performed by phenotypic methods that are often slow and cumbersome, despite the availability of more comprehensive genotyping technologies. we present an approach based on whole-genome amplification and resequencing microarrays for unbiased pathogen detection. this 10 h process identifies a broad spectrum of bacterial and viral species and predicts antibiotic resistance and pathogenicity and virulence profiles. we successfully ... | 2008 | 21261824 |
| decreasing enterobacter sakazakii (cronobacter spp.) food contamination level with bacteriophages: prospects and problems. | enterobacter sakazakii (cronobacter spp.) is an opportunistic pathogen, which can cause rare, but life-threatening infections in neonates and infants through feeding of a contaminated milk formula. we isolated 67 phages from environmental samples and tested their lytic host range on a representative collection of 40 e. sakazakii strains. a cocktail of five phages prevented the outgrowth of 35 out of 40 test strains in artificially contaminated infant formula. two e. sakazakii phages represented ... | 2008 | 21261874 |
| linear alkylbenzene sulfonate tolerance in bacteria isolated from sediment of tropical water bodies polluted with detergents. | the discharge of untreated detergent-bearing waste introduces linear alklcylbenzene sulfonates (las) to the aquatic environment. the surfactant persists in some streams and rivers in nigeria, some is adsorbed to suspended materials and end in the sediment of the receiving water bodies. in this study, bacteria isolated from sediments of some tropical detergent-effluent-polluted streams were tested for tolerance to las using the media dilution technique. las-tolerance was indicated by growth of th ... | 2008 | 19419067 |
| outer membrane protein a expression in enterobacter sakazakii is required to induce microtubule condensation in human brain microvascular endothelial cells for invasion. | enterobacter sakazakii (es) causes neonatal meningitis and necrotizing enterocolitis with case-fatality rates among infected infants ranging from 40 to 80%. very little is known about the mechanisms by which these organisms cause disease. here, we demonstrate that es invades human brain microvascular endothelial cells (hbmec) with higher frequency when compared with epithelial cells and endothelial cells from different origins. the entry of es into hbmec requires the expression of outer membrane ... | 2008 | 18606523 |
| insights into the catalytic mechanism of tyrosine phenol-lyase from x-ray structures of quinonoid intermediates. | amino acid transformations catalyzed by a number of pyridoxal 5'-phosphate (plp)-dependent enzymes involve abstraction of the calpha proton from an external aldimine formed between a substrate and the cofactor leading to the formation of a quinonoid intermediate. despite the key role played by the quinonoid intermediates in the catalysis by plp-dependent enzymes, limited accurate information is available about their structures. we trapped the quinonoid intermediates of citrobacter freundii tyros ... | 2008 | 18715865 |
| plasmid-borne arma methylase gene, together with blactx-m-15 and blatem-1, in a klebsiella oxytoca isolate from china. | an arma-producing klebsiella oxytoca isolate, strain 157, was detected after screening of 447 extended-spectrum beta-lactamase-producing enterobacteriaceae isolates in china. k. oxytoca 157 was resistant to aminoglycosides, ciprofloxacin and most beta-lactams. resistance to aminoglycosides and beta-lactams could be transferred to recipient escherichia coli by conjugation. arma, blactx-m-15 and blatem-1 genes were detected in k. oxytoca 157 and transconjugant e. coli strain 600(pec157). mutation ... | 2008 | 18809557 |
| infective arthritis: bacterial 23s rrna gene sequencing as a supplementary diagnostic method. | consecutively collected synovial fluids were examined for presence of bacterial dna (a 700-bp fragment of the bacterial 23s rrna gene) followed by dna sequencing of amplicons, and by conventional bacteriological methods. one or more microorganisms were identified in 22 of the 227 synovial fluids (9,7%) originating from 17 patients. sixteen of the patients had clinical signs of arthritis. for 11 patients molecular and conventional bacterial examinations were in agreement. staphylococcus aureus, s ... | 2008 | 19088916 |
| landscape phage as a molecular recognition interface for detection devices. | filamentous phages are thread-shaped bacterial viruses. their outer coat is a tube formed by thousands equal copies of the major coat protein pviii. libraries of random peptides fused to pviii domains were used for selection of phages probes specific for a panel of test antigens and biological threat agents. because the viral carrier in the phage borne bio-selective probes is infective, they can be cloned individually and propagated indefinitely without needs of their chemical synthesis or recon ... | 2008 | 19190724 |
| amplification-based dna analysis in the diagnosis of prosthetic joint infection. | microbiological cultures are moderately sensitive for diagnosing prosthetic joint infection (pji). this study was conducted to determine whether amplification-based dna methods applied on intraoperative samples could enhance pji diagnosis compared with culture alone in routine surgical practice. revision arthroplasty was performed for suspected pji (n = 41) and osteoarthrosis control (n = 28) patients, and a diagnosis of pji was confirmed in 34 patients. amplification by polymerase chain reactio ... | 2008 | 18832459 |
| transfer of antibiotic multiresistant plasmid rp4 from escherichia coli to activated sludge bacteria. | in situ transfer of a self-transmissible, antibiotic-multiresistant plasmid rp4 from a laboratory escherichia coli strain c600 to indigenous activated sludge bacteria was investigated using filter mating. the transfer frequency of rp4 from the donor e. coli to the bacteria that was sampled from two wastewater treatment plants was 5.1x10(-2) to 7.5x10(-1) and 4.6x10(-3) to 1.3x10(-2)/potential recipient. the isolated transconjugants showed resistance to ap, km, and tc and the presence of a plasmi ... | 2008 | 18930008 |
| [retropharyngeal abscess revealing diabetes: a case report]. | retropharyngeal abscess, a rare event in adults, is usually observed in immunodeficient persons or subsequent to foreign bodies. we report a case of retropharyngeal abscess which revealed diabetes mellitus. a 47-year-old woman with an uneventful history complained of fever, dysphagia and trismus. clinical findings were swelling of the pharyngeal posterior wall. the ct scan showed an abscess in the retropharyngeal space. puncture revealed the purulent nature of the collection. the bacteriological ... | 2008 | 18930451 |
| activation of beta1,3-n-acetylglucosaminyltransferase-2 (beta3gn-t2) by beta3gn-t8. possible involvement of beta3gn-t8 in increasing poly-n-acetyllactosamine chains in differentiated hl-60 cells. | enzymatic activities of some glycosyltransferases are markedly increased via complex formation with other transferases or cofactor proteins. we previously showed that beta1,3-n-acetylglucosaminyltransferase-2 (beta3gn-t2) and beta3gn-t8 can form a heterodimer in vitro and that the complex exhibits much higher enzymatic activity than either enzyme alone (seko, a., and yamashita, k. (2005) glycobiology 15, 943-951). here we examined this activation and the biological significance of complex format ... | 2008 | 18826941 |
| high prevalence of plasmid-mediated quinolone resistance genes qnr and aac(6')-ib-cr in clinical isolates of enterobacteriaceae from nine teaching hospitals in china. | quinolone resistance is an emerging problem in china. to investigate the prevalence of the plasmid-mediated quinolone resistance genes qnr and aac(6')-ib-cr, a total of 265 clinical isolates of escherichia coli, klebsiella pneumoniae, citrobacter freundii, and enterobacter cloacae with ciprofloxacin mics of > or =0.25 microg/ml were screened at nine teaching hospitals in china. the qnra, qnrb, qnrs, and aac(6')-ib genes were detected by pcr. the aac(6')-ib-cr gene was further identified by diges ... | 2008 | 18809939 |
| effects of therapeutic ceftiofur administration to dairy cattle on escherichia coli dynamics in the intestinal tract. | the goal of this study was to follow ceftiofur-treated and untreated cattle in a normally functioning dairy to examine enteric escherichia coli for changes in antibiotic resistance profiles and genetic diversity. prior to treatment, all of the bacteria cultured from the cows were susceptible to ceftiofur. ceftiofur-resistant e. coli was only isolated from treated cows during and immediately following the cessation of treatment, and the 12 bla(cmy-2)-positive isolates clustered into two genetic g ... | 2008 | 18820057 |
| bacterial microcompartments: their properties and paradoxes. | many bacteria conditionally express proteinaceous organelles referred to here as microcompartments (fig. 1). these microcompartments are thought to be involved in a least seven different metabolic processes and the number is growing. microcompartments are very large and structurally sophisticated. they are usually about 100-150 nm in cross section and consist of 10,000-20,000 polypeptides of 10-20 types. their unifying feature is a solid shell constructed from proteins having bacterial microcomp ... | 2008 | 18937343 |
| photorhabdus luminescens genes induced upon insect infection. | photorhabdus luminescens is a gram-negative luminescent enterobacterium and a symbiote to soil nematodes belonging to the species heterorhabditis bacteriophora. p.luminescens is simultaneously highly pathogenic to insects. this bacterium exhibits a complex life cycle, including one symbiotic stage characterized by colonization of the upper nematode gut, and a pathogenic stage, characterized by release from the nematode into the hemocoel of insect larvae, resulting in rapid insect death caused by ... | 2008 | 18489737 |
| enterohaemorrhagic escherichia coli and shigella dysenteriae type 1-induced haemolytic uraemic syndrome. | haemolytic uraemic syndrome (hus) can be classified according to the aetiology of the different disorders from which it is composed. the most prevalent form is that induced by shigatoxin producing escherichia coli (stec) and, in some tropical regions, by shigella dysenteriae type 1. stec cause a zoonosis, are widely distributed in nature, enter the food chain in different ways, and show regional differences. not all stec are human pathogens. enterohaemorrhagic e. coli usually cause attachment an ... | 2008 | 18493800 |
| direct comparison of the bd phoenix system with the microscan walkaway system for identification and antimicrobial susceptibility testing of enterobacteriaceae and nonfermentative gram-negative organisms. | the phoenix automated microbiology system (bd diagnostics, sparks, md) is designed for the rapid identification (id) and antimicrobial susceptibility testing (ast) of clinically significant human bacterial pathogens. we evaluated the performance of the phoenix instrument in comparison with that of the microscan walkaway system (dade behring, west sacramento, ca) in the id and ast of gram-negative clinical strains and challenge isolates of enterobacteriaceae (n = 150) and nonfermentative gram-neg ... | 2008 | 18495856 |
| interrupted time series analysis of vancomycin compared to cefuroxime for surgical prophylaxis in patients undergoing cardiac surgery. | the increased incidence of methicillin-resistant staphylococcus aureus (mrsa), the emergence of community-acquired mrsa, and the continued high incidence of methicillin-resistant staphylococcus epidermidis have required that certain institutions choose vancomycin for surgical prophylaxis. however, the data supporting the use of vancomycin for surgical prophylaxis are controversial. the purpose of this project was to assess the effect of the change from cefuroxime to vancomycin for surgical site ... | 2008 | 18025116 |
| interrupted time series analysis of vancomycin compared to cefuroxime for surgical prophylaxis in patients undergoing cardiac surgery. | the increased incidence of methicillin-resistant staphylococcus aureus (mrsa), the emergence of community-acquired mrsa, and the continued high incidence of methicillin-resistant staphylococcus epidermidis have required that certain institutions choose vancomycin for surgical prophylaxis. however, the data supporting the use of vancomycin for surgical prophylaxis are controversial. the purpose of this project was to assess the effect of the change from cefuroxime to vancomycin for surgical site ... | 2008 | 18025116 |
| microbiological implications of periurban agriculture and water reuse in mexico city. | recycled treated or untreated wastewater represents an important health challenge in developing countries due to potential water related microbiological exposure. our aim was to assess water quality and health implications in a mexico city periurban agricultural area. | 2008 | 18509453 |
| citrobacter freundii peritonitis and tunnel infection in a patient on continuous ambulatory peritoneal dialysis. | the clinical course of a patient on continuous ambulatory peritoneal dialysis who developed peritonitis and tunnel infection due to an unusual pathogen, citrobacter freundii, is described. the patient did not respond well to antibiogram-based therapy (intravenous meropenem and intraperitoneal gentamicin) and removal of the catheter was required. | 2008 | 18065679 |
| enterobacter sakazakii invasion in human intestinal caco-2 cells requires the host cell cytoskeleton and is enhanced by disruption of tight junction. | enterobacter sakazakii is an opportunistic pathogen that causes systemic bacteremia and meningitis with high mortality, and powdered infant formula is a frequent source of this bacterium. however, the mechanisms that this organism uses to invade and translocate through the intestinal barrier are unknown. using caco-2 epithelial cells, we were able to demonstrate penetration of e. sakazakii and to determine invasion-associated properties. we found that e. sakazakii entry and invasion were depende ... | 2008 | 18070906 |
| comparison of tetraploid blastocyst microinjection of outbred crl:cd1(icr), hybrid b6d2f1/tac, and inbred c57bl/6ntac embryos for generation of mice derived from embryonic stem cells. | embryo electrofusion and tetraploid blastocyst microinjection is a modification of the traditional embryonic stem cell (es cell)-based method to generate targeted mutant mice. viability of tetraploid embryos is reportedly lower than with diploid embryos, with considerable interstrain variation. here we assessed fetus and pup viability after es cell microinjection of tetraploid blastocysts derived from outbred, hybrid, and inbred mice. two-cell mouse embryos (c57bl/6ntac [b6], n = 788; b6d2f1/tac ... | 2008 | 18524172 |
| how bacteria consume their own exoskeletons (turnover and recycling of cell wall peptidoglycan). | summary: the phenomenon of peptidoglycan recycling is reviewed. gram-negative bacteria such as escherichia coli break down and reuse over 60% of the peptidoglycan of their side wall each generation. recycling of newly made peptidoglycan during septum synthesis occurs at an even faster rate. nine enzymes, one permease, and one periplasmic binding protein in e. coli that appear to have as their sole function the recovery of degradation products from peptidoglycan, thereby making them available for ... | 2008 | 18535144 |
| evaluation of reuterin production in urogenital probiotic lactobacillus reuteri rc-14. | classified as a distinct species in 1980, lactobacillus reuteri strains have been used in probiotic formulations for intestinal and urogenital applications. in the former, the primary mechanism of action of l. reuteri sd2112 (atcc 55730) has been purported to be its ability to produce the antibiotic 3-hydroxypropionaldehyde (3-hpa), also known as reuterin. in the vagina, it has been postulated that probiotic lactobacillus reuteri rc-14 does not require reuterin production but mediates a restorat ... | 2008 | 18539802 |
| sensitive and rapid detection of cholera toxin-producing vibrio cholerae using a loop-mediated isothermal amplification. | vibrio cholerae is widely acknowledged as one of the most important waterborne pathogen causing gastrointestinal disorders. cholera toxin (ct) is a major virulence determinant of v. cholerae. detection of ct-producing v. cholerae using conventional culture-, biochemical- and immunological-based assays is time-consuming and laborious, requiring more than three days. thus, we developed a novel and highly specific loop-mediated isothermal amplification (lamp) assay for the sensitive and rapid detec ... | 2008 | 18547441 |
| enterobacter sakazakii invasion in human intestinal caco-2 cells requires the host cell cytoskeleton and is enhanced by disruption of tight junction. | enterobacter sakazakii is an opportunistic pathogen that causes systemic bacteremia and meningitis with high mortality, and powdered infant formula is a frequent source of this bacterium. however, the mechanisms that this organism uses to invade and translocate through the intestinal barrier are unknown. using caco-2 epithelial cells, we were able to demonstrate penetration of e. sakazakii and to determine invasion-associated properties. we found that e. sakazakii entry and invasion were depende ... | 2008 | 18070906 |
| il-6-dependent mucosal protection prevents establishment of a microbial niche for attaching/effacing lesion-forming enteric bacterial pathogens. | enteric infections with attaching/effacing lesion-inducing bacterial pathogens are a worldwide health problem. a murine infection model with one such pathogen, citrobacter rodentium, was used to elucidate the importance of the pleiotropic immune regulator, il-6, in the pathogenesis of infection. il-6 was strongly induced in colonic epithelial cells and macrophages upon c. rodentium infection and was required for effective host defense, because mice lacking il-6 failed to control bacterial number ... | 2008 | 18453602 |
| exposure-response analyses of tigecycline efficacy in patients with complicated intra-abdominal infections. | exposure-response analyses were performed to test the microbiological and clinical efficacies of tigecycline in complicated intra-abdominal infections where escherichia coli and bacteroides fragilis are the predominant pathogens. data from evaluable patients enrolled in three clinical trials were pooled. patients received intravenous tigecycline (100-mg loading dose followed by 50 mg every 12 h or 50-mg loading dose followed by 25 mg every 12 h). at the test-of-cure visit, microbiological and cl ... | 2008 | 17954694 |
| a convenient microbiological assay employing cell-free extracts for the rapid characterization of gram-negative carbapenemase producers. | the dissemination of metallo and serine carbapenem-hydrolysing beta-lactamases among gram-negative nosocomial bacteria represents an acute problem worldwide. here, we present a rapid and sensitive assay for the characterization of carbapenemase producers to aid in infection control and prevention. | 2008 | 18456651 |
| isolation and characterization of tetracycline-resistant citrobacter spp. from catfish. | fifty-two tetracycline-resistant citrobacter spp. strains were isolated from farm-raised catfish. morphological and biochemical characteristics indicated that 38 of the 52 citrobacters were citrobacter freundii, 7 were c. amalonaticus and 7 were c. braakii. all isolates were resistant to multiple antibiotics. polymerase chain reaction (pcr) protocols were developed to detect the presence of 3 tetracycline-resistance genes (teta, tetb and tetg) from citrobacter isolates. oligonucleotide primers s ... | 2008 | 17993380 |
| molecular survey of beta-lactamases conferring resistance to newer beta-lactams in enterobacteriaceae isolates from polish hospitals. | the first national survey of resistance to newer beta-lactams in nosocomial populations of enterobacteriaceae in poland was performed. the study covered all nonrepetitive enterobacterial isolates cultured from specimens from inpatients in 13 regional secondary-care hospitals from november 2003 to january 2004. among 2,388 isolates, the predominant species was escherichia coli (59.6%), followed by proteus mirabilis (14.5%) and klebsiella spp. (8.5%). the frequency of extended-spectrum beta-lactam ... | 2008 | 18458126 |
| rapid polymerase chain reaction-based screening assay for bacterial biothreat agents. | to design and evaluate a rapid polymerase chain reaction (pcr)-based assay for detecting eubacteria and performing early screening for selected class a biothreat bacterial pathogens. | 2008 | 18370996 |
| structure of a phosphoethanolamine-containing o-polysaccharide of citrobacter freundii strain pcm 1443 from serogroup o39 and its relatedness to the klebsiella pneumoniae o1 polysaccharide. | lipopolysaccharide was extracted from cells of citrobacter freundii pcm 1443 from serogroup o39 and degraded by mild acid hydrolysis to give an o-polysaccharide. based on enzymatic and methylation analyses, along with 1h and 13c nuclear magnetic resonance spectroscopy, it was found that the lipopolysaccharide studied has two different linear polysaccharide chains of d-galactan type containing 3-substituted galactose residues. one of the galactans has the disaccharide repeating units of alpha-d-g ... | 2008 | 18371072 |
| pericardial effusion as the only manifestation of infection with francisella tularensis: a case report. | francisella tularensis, a facultative intracellular gram-negative bacterium, has rarely been reported as an agent of pericarditis, generally described as a complication of tularemia sepsis. f. tularensis is a fastidious organism that grows poorly on standard culture media and diagnosis is usually based on serological tests. however, cross-reactions may occur. western blotting allows the correct diagnosis. | 2008 | 18554395 |
| optimal probe length and target location for electrochemical detection of selected uropathogens at ambient temperature. | we have previously demonstrated the clinical validity of the rapid detection of uropathogens by use of a dna biosensor. this assay involves the hybridization of capture and detector probe pairs with bacterial 16s rrna target molecules to form a dna-rna sandwich on the sensor surface. horseradish peroxidase-conjugated antibody binds to the detector probe to enzymatically amplify the hybridization signal. these previous studies involved the hybridization of bacterial 16s rrna target sequences with ... | 2008 | 18562584 |