Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| features of an autoimmune process in mid-dermal elastolysis. | mid-dermal elastolysis is a rare disorder that is manifested clinically by wrinkling and histologically by the selective absence of elastic fibers in the mid dermis. we describe a young woman who developed abnormal wrinkling after augmentation mammoplasty with silicone implants. histologic examination confirmed the diagnosis of mid-dermal elastolysis. in addition, a positive antinuclear antibody titer and a false-positive lyme titer were subsequently detected. in a previously reported case a pat ... | 1992 | 1469138 |
| [lyme disease with exclusively neurologic manifestations]. | 1992 | 1507904 | |
| furor at lyme disease conference. | 1992 | 1604309 | |
| lyme disease in ireland. | the data pertaining to irish specimens sent to the lyme disease laboratory at charing cross hospital since 1986 is presented and discussed. in the period up to june 1990, 484 specimens were tested, 14% of these were positive by enzyme linked immunosorbent assay or indirect immunofluorescent assay. only 13 of these were confirmed as positive by immunoblotting. | 1992 | 1628946 |
| an update on lyme disease in pennsylvania. | 1992 | 1614712 | |
| lyme disease? | 1992 | 1596214 | |
| lyme disease. | 1992 | 27237181 | |
| is it lyme disease? how to interpret results of laboratory testing. | although laboratory testing is likely to have a greater role in diagnosis and monitoring of treatment of lyme disease in the future, at present physicians must rely on a combination of history taking, clinical manifestations, and laboratory results. unwarranted antibiotic therapy is to be avoided. asymptomatic persons with positive serologic results who live in an endemic area present a challenge, and borderline serologic results in patients with manifestations of late lyme disease are also trou ... | 1992 | 1589367 |
| lyme disease in maryland: 1987-1990. | we describe the epidemiology of lyme disease in maryland for 1987 to 1990 when the number of cases reported grew from 23 to 448 and the number of cases meeting the cdc case definition grew from 23 to 238, as well as discuss the implications of increased reporting and diagnosis of lyme disease. | 1992 | 1625531 |
| chronic lyme disease arthritis: review of the literature and report of a case of wrist arthritis. | a case of lyme arthritis with advanced degenerative changes localized to the midcarpal joint was treated with a limited wrist arthrodesis with relief of pain and improved function. chronic lyme arthritis occurs as the third stage of lyme disease. serologic testing and a history of a characteristic rash may be helpful in the diagnosis. radiographic and histopathologic findings are nonspecific, with both degenerative and inflammatory characteristics. intravenous antibiotics provide an effective tr ... | 1992 | 1613242 |
| ultrasonography for the diagnosis of lyme disease in cases of acute facial paralysis. | ultrasonography of the parotid gland was introduced in 1989 as part of the diagnostic protocol of every patient treated in our clinics with acute facial paralysis. ten of 50 patients so tested were found to have unilateral nonpalpable enlarged lymph nodes in the caudal portion of the parotid gland around the stylomastoid foramen. all 10 patients were eventually diagnosed as having lyme disease. it is suggested that in patients with acute facial paralysis, ultrasonography is an inexpensive tool t ... | 1992 | 1573949 |
| lyme disease and radiologic findings in lyme arthritis. | lyme disease typically begins in the spring or summer months, with a pathognomonic skin lesion and associated flulike or meningitislike symptoms. if the patient is not treated during this early stage, cardiac, neurologic, or musculoskeletal manifestations may occur weeks to months later. although the clinical picture of lyme disease is extremely variable, the diagnosis can be made in most cases by recognizing the typical patterns of organ involvement and associated immunologic abnormalities. | 1992 | 1566669 |
| how to detect lyme disease. | if patients lack the characteristic sign of lyme disease-a red rash with a pale center-diagnosing the disease can be challenging. a thorough workup and history should alert physicians when patients' flulike symptoms are the result of this tick-borne infection. | 1992 | 27424646 |
| lyme disease acquired outside of japan. | 1992 | 1633372 | |
| false positive reactions occurring with the captia syphilis-g eia, in sera from patients with lyme disease. | 1992 | 1582661 | |
| foot and ankle pain resulting from rheumatic conditions. | pain in the foot and ankle is most frequently secondary to static and degenerative changes, eg, corns, hammer toes, bunions, anterior metatarsalgia, and heel pain. a second common group consists of rheumatologic disorders that encompass immune and hereditary factors. this group includes rheumatoid arthritis, the often underdiagnosed seronegative spondyloarthropathies, and, less commonly, crystalline deposit disorders and diffuse connective tissue diseases. both the physician and the public need ... | 1992 | 1581152 |
| diagnosing lyme disease. | 1992 | 1543317 | |
| [comment on the contribution by c. garbe: borreliosis of the skin--progress of knowledge since the discovery of lyme disease]. | 1992 | 1577606 | |
| [oligosymptomatic lyme disease. diagnosis in primary care]. | 1992 | 1576196 | |
| prospective evaluation of heart block complicating early lyme disease. | lyme disease is a recognized cause of heart block/carditis. the incidence of heart block complicating early lyme disease has not been prospectively evaluated. in this study, 61 patients with early lyme disease documented by the rash of erythema migrans were prospectively evaluated for carditis. fifty five of 61 patients had a repeat examination 3 to 4 weeks after initiation of antibiotic therapy. only one of 61 patients (1.6%) presented with heart block, which resolved with antibiotics. none of ... | 1992 | 1372717 |
| difficulties with lyme serology. | lyme disease is a multisystem infection characterized by dermatologic, neurologic, and arthritic findings. like syphilis, lyme borreliosis may imitate several other infectious and non-infectious diseases. diagnosis is dependent on a reliable history (if available), clinical findings, and blood serology findings. a major problem has been seronegativity in persons possessing the disease (false negatives). at present, seronegativity in persons strongly suspected of having lyme disease does not nece ... | 1992 | 1583267 |
| minocycline in lyme disease. | 1992 | 1552067 | |
| [lyme disease in spain]. | 1992 | 1552758 | |
| the neuropsychiatric manifestations of lyme borreliosis. | lyme borreliosis (lyme disease), a tick-borne spirochetal illness, has multisystemic involvement and is rapidly increasing in certain areas of the united states. although its neurologic manifestations are becoming increasingly well recognized, its psychiatric presentations are not well known. the first section of this paper will provide an overview of lyme borreliosis and a review of the relevant neuropsychiatric literature. the second section will provide clinical descriptions of some common ne ... | 1992 | 1438607 |
| treatment of lyme disease. | 1992 | 1406447 | |
| rheumatology--lyme disease. | 1992 | 1468248 | |
| the epidemiology of lyme borreliosis in italy. | erythema migrans was described in italy only in 1971 although italian dermatologists were familiar with it a long time before. in 1983, the first case of lyme borreliosis with multisystem involvement was identified. the endemic areas in italy are the ligurian coast, the province of friuli venezia giulia, and the region surrounding the town of bologna. in liguria, the incidence of lyme disease is about 17/100,000 inhabitants per year. serosurveys of the general population and of sentinel animals ... | 1992 | 1435355 |
| lyme arthritis as the incorrect diagnosis in pediatric and adolescent fibromyalgia. | in areas endemic for lyme disease there is increasing concern and anxiety about possible chronic and untreatable manifestations of the disease. the authors have diagnosed fibromyalgia in many patients with chronic musculoskeletal complaints in whom chronic lyme arthritis had previously been diagnosed as the cause of their joint pains. fibromyalgia is a common disorder, causing arthralgia (not true arthritis), fatigue, and debility. the repeated and/or long-term antibiotic therapy prescribed for ... | 1992 | 1408503 |
| lyme disease: asking the right questions. | 1992 | 1411496 | |
| progressive hemifacial atrophy associated with lyme disease. | a case is described of progressive hemifacial atrophy occurring in a child with lyme disease. borrelial infection may have been an etiologic factor in the progressive hemifacial atrophy, which was ultimately treated with an siea free-flap transfer. | 1992 | 1513894 |
| not everything that glitters is lyme disease. | 1992 | 1524093 | |
| lyme disease revisited. | 1992 | 1520077 | |
| neurologic lyme disease. | 1992 | 1455107 | |
| reported cases of lyme disease in kansas. | 1992 | 1447863 | |
| field ecology of lyme disease in kansas. | 1992 | 1447862 | |
| prevalence of lyme disease infection in oklahoma. | even though lyme disease probably existed in oklahoma prior to 1975 when the disease was first reported in connecticut and even though ecological conditions favor a high prevalence, an unexpectedly low rate was evidenced in both a serological survey and the number of cases reported to the oklahoma state department of health. lyme disease does not appear to pose a significant public health threat in oklahoma at the present time; however, the the presence of all the necessary factors for transmiss ... | 1992 | 1404480 |
| lyme disease in israel. | 1992 | 1401296 | |
| lyme disease during pregnancy. acog committee opinion: committee on obstetrics: maternal and fetal medicine. number 99--november 1991. | 1992 | 1358705 | |
| the origin of plagues: old and new. | viruses and bacteria emerge in new and old forms to cause disease epidemics. some microorganisms recur when changing life-styles (including increased international travel) offer new opportunities; others arise from new genetic variations. these various epidemics connect the future with the past, offering lessons for guarding the health of generations to come--lessons learned from diseases such as tuberculosis, toxic shock syndrome, lyme disease, streptococcal infection, influenza, and acquired i ... | 1992 | 1509258 |
| erythema migrans in lyme disease: a correction. | 1992 | 1640613 | |
| comparison of cefuroxime axetil and doxycycline in the treatment of early lyme disease. | to compare the efficacy of cefuroxime axetil and doxycycline in the treatment of patients with lyme disease associated with erythema migrans. | 1992 | 1637021 |
| epidemiology, clinical features, and diagnosis of lyme disease. | lyme disease is the most common vector-borne disease in the united states. epidemiologic research has contributed to a better understanding of the factors involved in the geographic spread and increased incidence of the disease. clinical features of lyme disease, including skin, joint, cardiac, and neurologic involvement, are discussed here, along with recent studies that better characterize these clinical manifestations and more clearly define their immunopathogenesis. the diagnosis of lyme dis ... | 1992 | 1503876 |
| how to diagnose and treat lyme disease in children. infectious diseases and immunization committee, canadian paediatric society. | 1992 | 1623463 | |
| diagnosis of lyme disease in children. | 1992 | 1625075 | |
| memory impairment and depression in patients with lyme encephalopathy: comparison with fibromyalgia and nonpsychotically depressed patients. | lyme encephalopathy, primarily manifested by disturbances in memory, mood, and sleep, is a common late neurologic manifestation of lyme disease. we compared 20 patients with lyme encephalopathy with 11 fibromyalgia patients and 11 nonpsychotically depressed patients using the california verbal learning test, wechsler memory scale, rey-osterrieth complex figure test, minnesota multiphasic personality inventory (mmpi), and beck depression inventory. compared with patients with fibromyalgia or depr ... | 1992 | 1620329 |
| lyme disease--a pediatric perspective. | 1992 | 1527779 | |
| clinical manifestations and antibiotic treatment of lyme disease. | as the incidence of lyme disease increases in connecticut and world-wide, considerable attention has been given to its prompt diagnosis and treatment. creating further interest in this infection is the awareness that inappropriate therapy may result in significant disabling sequelae many years later. in this review, we focus mainly on current treatment options, but stress that the recommendations may change appreciably, as more information appears on the efficacy of new antibiotics. | 1992 | 1424603 |
| lyme disease in michigan. an update. | 1992 | 1406352 | |
| an investigation of a workplace cluster of bell's palsy. | we undertook a study to confirm the existence of an apparent cluster of bell's palsy in an industrial plant ("w4"), and to test etiologic hypotheses regarding chemical exposures, immune suppression, and infectious etiologies. cases were enumerated by questionnaire. employees with a history of bell's palsy ("cases") were invited to participate in a study that included medical history, t cell studies, viral and lyme disease serologies, and blink reflex studies. thirty-three unaffected volunteers f ... | 1992 | 1432295 |
| classification of italian isolates of borrelia burgdorferi into three genomic groups. | in this study we investigated the genotypic characteristics of some locally isolated strains of b. burgdorferi by three different methodologies: restriction endonuclease analysis (rea), southern blot hybridization with whole dnas from borrelia strains and southern blot hybridization with rrna 16 + 23s genes derived from e. coli. rea fingerprintings were evaluated by cluster analysis, according to the principles of numerical taxonomy. the genomas of the locally isolated strains were compared with ... | 1993 | 7505385 |
| organization of ribosomal rna genes in borrelia burgdorferi sensu lato isolated from ixodes ovatus in japan. | borrelia burgdorferi sensu lato was obtained from adult ixodid ticks, ixodes ovatus, collected in nagano, japan, and was named nt112. the genomic dna was digested with enzymes, electrophoresed, blotted and hybridized with rrna gene probes obtained from b. burgdorferi sensu stricto b31. the results showed that the borrelial chromosome contains a single rrs (16s rrna gene) sequence and two copies of rrl/rrf (23s/5s rrna genes) sequences. the rrl/rrf genes were tandemly repeated at intervals of 3.2 ... | 1993 | 7507551 |
| cross-reactive antigenic domains of the flagellin protein of borrelia burgdorferi. | the p41 flagellin of borrelia burgdorferi is the most common antigen recognized by serum of patients with lyme borreliosis. this antigen shares amino acid homology, particularly in the amino and carboxy termini, with periflagellar antigens found in other microorganisms including treponema pallidum. we cloned and expressed the p41 open reading frame in escherichia coli and expressed it both as trpe fusion and full-length unfused proteins. also, we generated deletion constructs of various portions ... | 1993 | 7504314 |
| the first isolation of borrelia burgdorferi from ixodes ricinus (acari, ixodidae) ticks in poland. | among ticks ixodes ricinus collected in april 1994 in the forest near fraknowo (olsztyn province, n. c. poland), 17 ticks were evaluated for borrelia infection by cultivating in the bsk-h medium (sigma). the ticks were examined in five pools--four of 3 females and one pool of 5 nymphs. spirochaetes were cultured successfully only from one pool of females and identified as borrelia burgdorferi with monoclonal antibodies: h 9724, h 5332, h 3ts and 11 g 1 by indirect immunofluorescence assay (ifa). ... | 1993 | 7580352 |
| relative utilization of reptiles and rodents as hosts by immature ixodes scapularis (acari: ixodidae) in the coastal plain of north carolina, usa. | the interaction of immature black-legged ticks, ixodes scapularis, with reptiles and rodents was investigated in various woodland habitats in the coastal plain of north carolina. reptiles were sampled from april 1 to september 30, 1991. no ticks were found on 95 specimens representing 16 species of snakes. ticks were found on 54 (36.7%) of 147 lizards. i. scapularis was the only tick recovered from lizards. some lizards were collected in drift fence traps each month of the study except august. c ... | 1993 | 7628223 |
| ability of transovarially and subsequent transstadially infected ixodes hexagonus ticks to maintain and transmit borrelia burgdorferi in the laboratory. | in a previous study, transstadial and transovarial survival of borrelia burgdorferi in ixodes hexagonus and transmission to laboratory mice via the bite of infected females were demonstrated. here, we report the ability of i. hexagonus progeny infected transovarially to maintain and transmit the spirochaete to the host. ticks were examined for spirochaetes by direct immunofluorescence antibody test. i. hexagonus larvae derived from the parental transstadially infected females were fed on two whi ... | 1993 | 7628234 |
| serologic response to the borrelia burgdorferi flagellin demonstrates an epitope common to a neuroblastoma cell line. | antibodies in sera of 7 patients with neurologic manifestations of lyme borreliosis and a monoclonal antibody (mab h9724) to the flagellin of borrelia burgdorferi have been shown to bind neural tissue. to identify the antibody binding site common to the b. burgdorferi flagellin and the neural tissue, we made recombinant fusion proteins expressing epitopes of flagellin. antibodies in patients' sera and mab h9724 bound within an 18-amino acid epitope (residues 208-225) in the central region of fla ... | 1993 | 7678336 |
| a monoclonal antibody to ospa inhibits association of borrelia burgdorferi with human endothelial cells. | previously, it has been shown that polyclonal antibodies to borrelia burgdorferi and some monoclonal antibodies (mabs) to borrelia major surface proteins caused inhibition of adherence of the bacteria to cultured human umbilical vein endothelial (huve) cells. in this study, fragment antigen binding (fab) molecules generated from the immunoglobulin g fraction of rabbit anti-recombinant ospa serum were found to inhibit the adherence of b. burgdorferi to huve cells by 73%. subsequently, mabs were g ... | 1993 | 7678585 |
| transcriptional analyses and mapping of the ospc gene in lyme disease spirochetes. | in lyme disease spirochetes, the ospc gene encodes a 22.7-kda protein referred to as either the pc or the ospc protein. using a variety of electrophoretic approaches followed by southern blotting and probing with oligonucleotide probes, we mapped the ospc gene to a circular 26-kb plasmid. the ospc gene represents the first gene to be mapped to a circular plasmid in lyme disease spirochetes. the occurrence of this gene in isolates belonging to each of the three lyme disease-associated species, bo ... | 1993 | 7679385 |
| preferential usage of t cell antigen receptor v region gene segment v beta 5.1 by borrelia burgdorferi antigen-reactive t cell clones isolated from a patient with lyme disease. | forty-three cd3+4+8- tcr alpha beta+ borrelia burgdorferi-reactive t cell clones isolated from the peripheral blood of a single patient with clinically active chronic lyme arthritis were characterized. the spirochetal ag recognized by 16 of these t cell clones was determined by reactivity with a panel of recombinant spirochetal ag, which included the ospa, ospb, flagellin, hsp60 and hsp70 proteins. all three t cell clones reactive with heat shock proteins recognized a non-cross-reactive epitope ... | 1993 | 7682589 |
| [reactive arthritis associated bacteria as the etiology of undifferentiated oligoarthritis]. | undifferentiated oligoarthritis (uoa) resembles clinically reactive arthritis (rea), but does not fulfill the diagnostic criteria. in 46 patients with uoa, in 16 with rea, and in 15 with rheumatoid arthritis (ra) the humoral and cellular immune response to the rea-associated bacteria chlamydia trachomatis, yersinia enterocolitica, shigella flexneri, salmonella enteritidis, campylobacter jejuni and borrelia burgdorferi was investigated in paired samples of synovial fluid and peripheral blood. an ... | 1993 | 7682746 |
| evasion of protective immunity by borrelia burgdorferi by truncation of outer surface protein b. | we analyzed variability in outer surface protein b (ospb) from borrelia burgdorferi (bb), the causative agent of lyme disease, to determine how bb escapes immune destruction. we have shown that vaccination with ospb from bb strain b31 protected mice from infection with bb b31 but not against bb n40. the present study demonstrates that bb n40 spirochetes which evade vaccination immunity to ospb have a truncated form of ospb, due to a taa stop codon at nucleotide 577. in contrast, bb n40 spirochet ... | 1993 | 7683420 |
| association of treatment-resistant chronic lyme arthritis with hla-dr4 and antibody reactivity to ospa and ospb of borrelia burgdorferi. | chronic lyme arthritis that is unresponsive to antibiotic therapy is associated with an increased frequency of the hla-dr4 specificity. to determine whether the immune response to a particular polypeptide of borrelia burgdorferi may be associated with treatment-resistant chronic lyme arthritis, we correlated the clinical courses and hla-dr specificities of 128 patients with lyme disease with their antibody responses to spirochetal polypeptides. antibody reactivity was determined by western blott ... | 1993 | 7685738 |
| analysis of borrelia species associated with lyme disease by rrna gene restriction fragment length polymorphism. | we investigated the usefulness of rrna gene restriction fragment length polymorphism (rflp) for grouping of the borrelia isolates associated with lyme disease or from ixodid ticks. genomic dna was digested with a restriction enzyme, blotted and hybridized with an rrl (23s rrna) gene probe. the sizes of the restriction bands showed a good correlation with the genotypes recently proposed, and borrelia isolates of diverse geographic origin formed four distinct dna groups. group i included all of th ... | 1993 | 7689632 |
| evaluation of genetic divergence of borrelial isolates from lyme disease patients in hokkaido, japan, by rrna gene probes. | eight spirochetal isolates (jem1 to jem8) were obtained from cutaneous lesions of patients with lyme disease in hokkaido, japan, and were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, reactivities with monoclonal and polyclonal antibodies, and southern blot hybridization. the protein profiles of these borrelial isolates were variable and differed markedly from that of borrelia burgdorferi b31. the 41-kda flagellin protein was present in all isolates, but the outer surfac ... | 1993 | 7690368 |
| evidence for an alpha-helical epitope on outer surface protein a from the lyme disease spirochete, borrelia burgdorferi: an application of steady-state and time-resolved fluorescence quenching techniques. | outer surface protein a (ospa) is a major antigen of borrelia burgdorferi, the etiological agent of lyme disease. a recombinant form of ospa (ospa-257) from b. burgdorferi, strain b31, contains 257 amino acids and a single tryptophan residue at position 216 (trp-216). mapping studies indicate that trp-216 is involved in the epitope for the agglutinating monoclonal antibody 105.5. however, the fluorescence emission maximum of the native protein is 330 nm, indicating that trp-216 is not solvent-ex ... | 1993 | 7691186 |
| species of borrelia distinguished by restriction site polymorphisms in 16s rrna genes. | three phyletic groups of borrelia associated with lyme disease, b. burgdorferi, b. garinii and group vs461 can be distinguished from each other and other species of borrelia by bfai restriction site polymorphisms in pcr amplified 16s rrna genes. one strain isolated from an ixodes pacificus tick in california that was previously unclassifiable was distinguishable from b. burgdorferi by an mn/i restriction site polymorphism. | 1993 | 7691681 |
| serologic analysis of dogs, horses, and cottontail rabbits for antibodies to an antigenic flagellar epitope of borrelia burgdorferi. | enzyme-linked immunosorbent assays (elisa) and immunoblots using either whole-cell lysates of borrelia burgdorferi or an antigenic region of flagellin (41-g) as the antigen were performed, and the abilities of the two assays to detect antibodies to this spirochete in dog, cottontail rabbit, and horse sera were compared. assays using whole-cell b. burgdorferi lysates as the antigen were more sensitive for detecting antibodies. elisa with 41-g as the antigen were specific for borrelia antibodies b ... | 1993 | 7691874 |
| [synovial cellular immune response to bacterial pathogens in patients with chronic juvenile arthritis]. | juvenile chronic arthritis is a heterogenous disease with an ill-defined pathogenesis. in our study, synovial fluid (sf) and peripheral blood (pb) of 70 children with oligoarthritis were investigated; bacteria-specific lymphocyte proliferation and antibodies to arthritogenic bacteria were determined. specific cellular immune responses in sf but not in pb were found in 4/7 patients with either lyme- or reactive arthritis (60%). in comparison, in subgroup jca ii (n = 45) encompassing mainly elder ... | 1993 | 7692686 |
| lyme disease: a review. | lyme disease is an uncommon multisystem spirochaetal infection that has attracted public and media attention in the united kingdom during the last few years. the spirochaete, borrelia burgdorferi, is transmitted through the bite of an infected tick. ticks are found throughout the uk in rural habitats and areas of urban parkland which attract many visitors. the illness can present with skin, nervous system, joint or other manifestations although infection may be asymptomatic. the risk of infectio ... | 1993 | 7693167 |
| detection of borrelia burgdorferi in tissues of experimentally infected peromyscus leucopus by the polymerase chain reaction. | a polymerase chain reaction (pcr) assay was used to amplify a 158 base pair (bp) dna fragment from the ospa locus of borrelia burgdorferi. this assay could identify all of four distinct b. burgdorferi isolates from oklahoma and could detect as little as 50 x 10(-15) g of purified dna from b. burgdorferi (jd-1) after agarose gel electrophoresis and ethidium bromide staining. the assay was used to detect the lyme spirochete in the heart, liver, spleen, kidney and urinary bladder of four experiment ... | 1993 | 7763705 |
| genetic diversity among borrelia burgdorferi isolates from wood rats and kangaroo rats in california. | twenty-nine borrelia burgdorferi isolates, obtained from dusky-footed wood rats (neotoma fuscipes) and california kangaroo rats (dipodomys californicus) in california, were analyzed genetically. chromosomal dna was examined by restriction endonuclease analysis (rea) and gene probe restriction fragment length polymorphism. pulsed-field gel electrophoresis was used to analyze the plasmid profiles of the isolates. rea, the method with the greatest discrimination, disclosed 24 distinct restriction p ... | 1993 | 7905880 |
| comparative analysis of genetic variability among borrelia burgdorferi isolates from europe and the united states by restriction enzyme analysis, gene restriction fragment length polymorphism, and pulsed-field gel electrophoresis. | the genomes of 62 north american and european borrelia burgdorferi isolates were examined by restriction endonuclease analysis (rea), gene probe restriction fragment length polymorphism, and pulsed-field gel electrophoresis (pfge). hybridization of restriction fragments with the immunologically relevant 83-kda antigen gene revealed polymorphisms and divided the isolates into three major groups. group i included all but two of the american isolates and some of the european isolates. one of two ca ... | 1993 | 7905881 |
| [the preliminary results of an ecological epidemiological study of lyme disease in western siberia]. | studies of ixodes persulcatus ticks in omsk, kurgan, and novosibirsk regions revealed natural foci of lyme's disease. vector infection rate varied from 21 to 23.5% in the omsk region, from 30 to 52.9% in the kurgan region, and from 15 to 58.3% in the novosibirsk region. serologic screening of inpatients hospitalized during the epidemic season of 1991 with suspected tick-borne encephalitis and lyme's disease confirmed the latter condition in 10.8% of the examinees in the omsk and in 10% in the ku ... | 1993 | 7968805 |
| genetic diversity among borrelia burgdorferi isolates: more than three genospecies? | in order to assess the genetic relationships among isolates of borrelia burgdorferi, we have determined chromosomal dna restriction fragment length polymorphisms (rflp) of 27 isolates. using five restriction enzymes and seven dna probes, we recorded 246 different hybridizing bands. the resulting matrix was subjected to factorial correspondence analysis and to phylogenetic analysis using a parsimony program. these analyses demonstrate two closely related genospecies and three individual strains. ... | 1993 | 7902598 |
| different genospecies of borrelia burgdorferi are associated with distinct clinical manifestations of lyme borreliosis. | borrelia burgdorferi sensu lato has been subdivided into three genospecies: b. burgdorferi sensu stricto, b. garinii, and b. burgdorferi group vs461. sixty-eight isolates cultured from patients and 26 strains from ticks were characterized with use of sds-page, western blotting, and rrna gene restriction analysis. fifty-seven of 58 strains obtained from the skin of 70 patients who had erythema migrams or acrodermatitis chronica atrophicans were of group vs461, whereas the genotype of the remainin ... | 1993 | 7903558 |
| characterization of borrelia species isolated from ixodid ticks, ixodes ovatus. | thirty-two borrelia isolates were obtained from the adult stage of ixodid ticks, ixodes ovatus, collected in various localities in japan. borrelial isolates were cultivated and analyzed by polyacrylamide gel electrophoresis, with monoclonal antibodies, by pulsed field gel electrophoresis, and by genomic southern hybridization. all borrelial isolates showed similar protein profiles and monoclonal antibody reactivities, while plasmid profiles were rather diverse. genomic hybridization using rrna g ... | 1993 | 7903788 |
| genomic analysis of borrelia japonica sp. nov. isolated from ixodes ovatus in japan. | genetic characteristics of 12 borrelia isolates from the tick, ixodes ovatus, i. persulcatus, and the rodent, apodemus speciosus ainu, in japan were compared to members of the three genospecies of borrelia burgdorferi sensu lato; b. burgdorferi sensu stricto, b. garinii and group vs461. the methods used in this study were the quantitative microplate dna hybridization assay and restriction fragment length polymorphism (rflp) analyses of the flagellin structural genes and the 16s rrna genes. the s ... | 1993 | 7905183 |
| [paralysis of abdominal muscles caused by lyme disease]. | a 67-year old patient with meningoradiculitis due to borrelia burgdorferi presented with unilateral trunk dysesthesias and severe asymmetrical abdominal distension. the causes of abdominal wall paralysis are reviewed. | 1993 | 7997746 |
| electron microscopy and the polymerase chain reaction of spirochetes from the blood of patients with lyme disease. | results of studies using direct antigen detection suggest that seronegative lyme borreliosis is not rare and support the hypothesis that borrelia antigens can persist in humans. we report three successful cultures from blood out of 30 attempts from 96 lyme disease patients. the proof of borreliaemia in early or late phases of lyme disease by immuno-capture electron microscopy has practical importance for subsequent cultivation. the polymerase chain reaction with oligonucleotide sequences directe ... | 1993 | 8004045 |
| the complex formation of influenza virus envelope glycoproteins with outer membrane proteins of neisseria meningitidis or borrelia burgdorferi. | the isolation of influenza virus envelope glycoproteins was achieved by one-step procedure consisting of treatment of purified virus with zwitterionic detergent and separation of viral constituents by sucrose density gradient centrifugation. viral glycoproteins and proteins of outer membrane of n. meningitidis or b. burgdorferi formed complexes after removal of the detergent by dialysis. complexing of viral glycoproteins and bacterial proteins was monitored by gel chromatography on sepharose 6b, ... | 1993 | 8010183 |
| uveitis and lyme borreliosis. | in a retrospective study 56 consecutive patients with uveitis of unknown origin and 56 consecutive patients suffering from uveitis of established aetiology were investigated. the purpose of this study was to determine the frequency of positive serological tests for lyme borreliosis among patients with uveitis and to relate laboratory data to clinical findings. the antibody titre for borrelia burgdorferi was determined by two assays: the indirect immunofluorescence assay and the enzyme linked imm ... | 1993 | 8025042 |
| lyme disease. | in 1992, reported lyme disease cases increased, but the majority remained clustered in the northeast and midwest, and in california. the clinical syndromes associated with infection are better understood, and this information can calm public anxiety. the concept of fibromyalgia occurring after infection with borrelia burgdorferi allows more appropriate treatment in late lyme disease. the polymerase chain reaction is being applied to gain insights into the pathogenic mechanisms of lyme disease. p ... | 1993 | 8043045 |
| [neurologic manifestations in lyme disease. apropos of 22 cases]. | the main clinical and biological features of 22 cases of neurological forms of lyme disease are reviewed. radiculitis (n = 15), cranial nevritis (n = 7), meningitis (n = 5) and encephalitis (n = 4) are often associated. tick bites were previously noticed in only 40% of cases; erythema chronicum migrans in 73%, "one to six months before the onset of neurological symptoms". titers of borrelia burgdorferi antibodies were always above 1/256. among 18 patients, dr w2 hla haplotype was present in 15. | 1993 | 8009078 |
| [epilepsy disclosing neuroborreliosis]. | after two seizures, a 13 year-old boy experienced headache, fatigue and loss of appetite over a period of 3 weeks. there was a bilateral papilledema with normal visual acuity. ct and mri disclosed two ischemic foci, that were interpreted as evidence of vasculitis. high serum levels of igg and igm antibodies specific to borrelia burgdorferi, were present. the patient had attended an outdoor scout camp in a area, in south-east belgium, known to be endemic for tick-born borreliosis. the clinical sy ... | 1993 | 8009148 |
| [borreliosis]. | borreliosis which incidence and epidemic range are constantly increasing is an infectious zoonosis, detected in the town of lyme, connecticut, usa in 1975. this disease is caused by borrelia burgdorferi transmitted by ticks. it relatively easily responds to antibiotic therapy. making of correct diagnosis, however, causes more problems. the disease has often atypical course, its phases overlap. it may also mimic collagen diseases and other spirochetoses. the relatively low specificity of serologi ... | 1993 | 8030328 |
| [a study on the relationship between cheilitis granulomatosa and melkersson-rosenthal syndrome]. | serum anti borrelia burgdorferi (bb) antibody of 18 patients with cheilitis granulomatosa (cg) and 5 patients with melkersson-rosenthal syndrome (mrs) have been examined and positive results are present in 14 patients with cg(77.8%) and 4 patients with mrs(80%). according to histopathological features the lesions show characteristics of spirochetes diseases. it suggests that cg and mrs are all caused by spirochetes. | 1993 | 8033637 |
| balance of synovial fluid il-1 beta and il-1 receptor antagonist and recovery from lyme arthritis. | borrelia burgdorferi, the causative agent of lyme disease, is a potent inducer of interleukin-1 beta (il-1 beta), a cytokine implicated in the pathogenesis of inflammatory arthritis. the balance between il-1 and the il-1 receptor antagonist (il-1ra), a naturally occurring inhibitor of il-1, might influence disease expression. to explore this possibility, we have done a retrospective study that compared the clinical course of lyme arthritis in 83 patients with concentrations of il-1 beta and il-1 ... | 1993 | 8093746 |
| infections in an alpine environment: antibodies to hantaviruses, leptospira, rickettsiae, and borrelia burgdorferi in defined italian populations. | from 1987 to 1991, a seroepidemiologic survey for antibodies to hantaviruses, leptospira, rickettsiae, and borrelia was conducted in selected italian population groups. in the mountainous areas of northeastern italy, the prevalence of antibody to hantaviruses, as detected by indirect immunofluorescent antibody (ifa) assay, was 7.1%, 4.8%, 4.3%, and 4% in 265 forestry workers, 82 rangers, 395 farmers, and 75 hunters, respectively. among 299 alpine soldiers, the prevalence was lower (0.7%). of tho ... | 1993 | 8093990 |
| acrodermatitis chronic atrophicans: a chronic t-cell-mediated immune reaction against borrelia burgdorferi? clinical, histologic, and immunohistochemical study of five cases. | acrodermatitis chronica atrophicans (aca) is a late manifestation of infection caused by borrelia burgdorferi. | 1993 | 8095272 |
| molecular mimicry in lyme disease: monoclonal antibody h9724 to b. burgdorferi flagellin specifically detects chaperonin-hsp60. | a monoclonal antibody (h9724), specific for the 41-kda flagellar protein of the lyme disease pathogen borrelia burgdorferi, cross-reacts with human axons and detects one major protein in human neuroblastoma cell extracts. the homologous cross-reacting protein has now been isolated from calf adrenal and identified as chaperonin-hsp60 by n-terminal sequencing. | 1993 | 8096152 |
| genetic heterogenity of the genes coding for the outer surface protein c (ospc) and the flagellin of borrelia burgdorferi. | the ospc gene coding for the outer surface protein ospc and the fla gene coding for the flagellin have been investigated in three different borrelia burgdorferi sensu lato strains. these strains (the north american strain b31 and the european strains pko and pbi) derive from various biological sources (lxodes dammini, human skin and human csf) and belong to three different b. burgdorferi ospa serotypes and genospecies (ospa serotype 1, b. burgdorferi sensu stricto; ospa serotype 2, group vs461 a ... | 1993 | 8098841 |
| immune recognition of human hsp60 by lyme disease patient sera. | members of the hsp60 family of microbial heat shock proteins frequently serve as immunodominant antigens and immunological responses to these highly conserved proteins have been implicated in the pathology of a number of autoimmune diseases and inflammatory processes associated with microbial infection. in the present study, sera from patients with lyme disease were examined by western blot analysis for the presence of igg against borrelia burgdorferi antigens and for autoreactive igg against re ... | 1993 | 8100982 |
| cross immunity among types of borrelia burgdorferi. | 1993 | 8101930 | |
| isolation of borrelia burgdorferi from myocardium. | 1993 | 8102439 | |
| linear chromosomal physical and genetic map of borrelia burgdorferi, the lyme disease agent. | a physical map of the 952 kbp chromosome of borrelia burgdorferi sh-2-82 has been constructed. eighty-three intervals on the chromosome, defined by the cleavage sites of 15 restriction enzymes, are delineated. the intervals vary in size from 96 kbp to a few hundred bp, with an average size of 11.5 kbp. a striking feature of the map is its linearity; no other bacterial groups are known to have linear chromosomes. the two ends of the chromosome do not hybridize with one another, indicating that th ... | 1993 | 8102774 |
| molecular and immunological characterization of a novel polymorphic lipoprotein of borrelia burgdorferi. | we describe the cloning, expression, and molecular characterization of a novel polymorphic borrelia burgdorferi lipoprotein recognized by monoclonal antibody la7. sequence analysis revealed an open reading frame encoding a 21,866-da polypeptide (ipla7). comparison with other known proteins indicated sequence similarity between ipla7 signal peptides and those of other prokaryotic lipoproteins, including the immunodominant b. burgdorferi outer surface proteins ospa, ospb, pc, and ospd. both natura ... | 1993 | 8104894 |
| genotypic and phenotypic diversity among nine swiss isolates of borrelia burgdorferi. | nine strains of borrelia burgdorferi isolated from ticks in the canton of valais (switzerland) were characterized genotypically by determining restriction fragment length polymorphisms (rflp) and plasmid profiles. the strains were also compared with respect to presence and electrophoretic mobility of the outer surface proteins ospa and ospb, and immunoreactivity of ospa and a 12 kd antigen. by both approaches, three different patterns were observed resulting in identical grouping of the strains. ... | 1993 | 8106023 |
| first isolation of borrelia burgdorferi from an iris biopsy. | the persistence of borrelia burgdorferi in six patients is described. borrelia burgdorferi has been cultivated from iris biopsy, skin biopsy, and cerebrospinal fluid also after antibiotic therapy for lyme borreliosis. lyme serology: igg antibodies to b. burgdorferi were positive, igm negative in four patients; in two patients both igm and igg were negative. antibiotic therapy may abrogate the antibody response to the infection as shown by our results. patients may have subclinical or clinical di ... | 1993 | 8106639 |
| complete heart block in a 9 year old girl caused by borreliosis. | a complete atrioventricular block was seen in a nine year old girl in whom an infection with borrelia burgdorferi was confirmed by serological testing. there were no other symptoms or cutaneous manifestations of the disease. though a rash on the right ear was later recalled by her parents. the patient was treated with high dose penicillin and orciprenaline was given intermittently. the complete heart block disappeared within four days. | 1993 | 8038006 |
| circulating borrelia burgdorferi in patients with acute lyme disease: results of blood cultures and serum dna analysis. | to determine the usefulness of blood culture and polymerase chain reaction (pcr) analysis in detecting circulating borrelia burgdorferi or its dna, blood and serum from untreated patients with acute lyme disease were examined. none of the cultures of blood or serum from the 7 patients tested demonstrated spirochetes. similarly, all patient serum samples, assayed in two laboratories, were negative for b. burgdorferi dna using pcr amplification. these results suggest that in patients with acute ly ... | 1993 | 8018136 |