Publications

serum levels of carboxyterminal cross-linked telopeptide of type i collagen (ictp) in the differential diagnosis of the syndromes of inappropriate secretion of tsh.	serum tsh assay is a very sensitive and specific index of thyroid hormone (th) action. nevertheless, in particular clinical situations, such as those of inappropriate tsh secretion, the measurement of additional parameters evaluating peripheral th action may be required in order to achieve a correct diagnosis and to assess the impact that thyroid hormone have on a given tissue. the availability of a specific ria for serum carboxy-terminal cross-linked telopeptide of type i collagen (ictp) prompt ...	1997	9302396
projections from the thoracic cord to the cerebellar nuclei in the rat, studied by anterograde axonal tracing.	the dorsal spinocerebellar tract (dsct) of the thoracic cord (the thoracic dsct) consists of uncrossed ascending axons originating from clarke's column, marginal neurons of clarke's column, and lamina v neurons, and crossed ascending axons originating from neurons in lamina viii and the ventromedial part of lamina vii. the present study has examined, by using anterograde labeling with biotinylated dextran, whether the thoracic dsct projects to the cerebellar nuclei. the tracer was injected into ...	1997	9303426
body temperature and endocrine interactions before and after calving in beef cows.	multiparous beef cows (n = 7) were used to evaluate peripartum changes and interactions among body temperature (bt) and circulating progesterone (p4), estradiol-17beta (e2), triiodothyronine (t3), cortisol, thyroxine (t4), and 13,14-dihydro-15-keto-prostaglandin f2alpha (pgfm) concentrations. electronic temperature monitors were placed under the obliquus abdominis internus muscle of the left flank, and bt was measured using radiotelemetry every 3 min for 10-s periods from 144 h before to 24 h af ...	1997	9303472
management of traumatic optic neuropathy with coexistent spinal cord injury: a case report.	traumatic optic neuropathy (ton) causes blindness of varied severity and occurs infrequently as a complication of closed head injury. a case is presented of ton that occurred in a patient who suffered complete t4 paraplegia from a motorcycle accident but in whom no severe head injury took place. in this case, high-dose intravenous methylprednisolone was begun for the spinal cord injury and repeated 24 hours later for the ton. vision improved from near total blindness to 20/400 in the left eye (o ...	1997	9305279
cloning of linear dnas in vivo by overexpressed t4 dna ligase: construction of a t4 phage hoc gene display vector.	a method was developed to clone linear dnas by overexpressing t4 phage dna ligase in vivo, based upon recombination deficient e. coli derivatives that carry a plasmid containing an inducible t4 dna ligase gene. integration of this ligase-plasmid into the chromosome of such e. coli allows standard plasmid isolation following linear dna transformation of the strains containing high levels of t4 dna ligase. intramolecular ligation allows high efficiency recircularization of cohesive and blunt-end t ...	1997	9305776
effect of melatonin on serum cholesterol and phospholipid levels, and on prolactin, thyroid-stimulating hormone and thyroid hormone levels, in hyperprolactinemic rats.	the effects of melatonin treatment and pituitary transplants on serum total and free cholesterol levels, cholesterol esterification index, phospholipid levels and prolactin, thyroid-stimulating hormone (tsh), thyroxine (t4) and triiodothyronine (t3) levels were examined in rats. male rats were grafted an anterior pituitary under the kidney capsule or were sham-operated on day 30th of life. thirty days later, the rats received 4 daily s.c. injections of melatonin (25, 50 or 100 microg/rat) or veh ...	1997	9307050
postoperative prognosis in patients with non-small cell lung cancer with synchronous ipsilateral intrapulmonary metastasis.	non-small cell lung cancer with intrapulmonary metastasis (pm) was recently reclassified according to the revision of the tnm classification. to determine whether the new staging system is appropriate, we analyzed the postoperative prognosis of patients with synchronously detected and resected pm lesions.	1997	9307478
molecular divergence of lysozymes and alpha-lactalbumin.	the vast number of proteins that sustain the currently living organisms have been generated from a relatively small number of ancestral genes that has involved a variety of processes. lysozyme is an ancient protein whose origin goes back an estimated 400 to 600 million years. this protein was originally a bacteriolytic defensive agent and has been adapted to serve a digestive function on at least two occasions, separated by nearly 40 million years. the origins of the related goose type and t4 ph ...	1997	9307874
results of a european randomized trial of etanidazole combined with radiotherapy in head and neck carcinomas.	the aim of the study was to evaluate the efficacy and toxicity of etanidazole, a hypoxic cell sensitizer, combined with radiotherapy in the treatment of head and neck squamous cell carcinoma.	1997	9308928
is prophylactic neck irradiation indicated in patients with squamous cell carcinoma of the maxillary sinus?	to determine the proportion of patients with squamous cell carcinoma of the maxillary sinus who will fail in regional nodes without elective neck treatment and to identify any prognostic factors that may influence neck control.	1997	9308929
microscopic local leptomeningeal invasion at diagnosis of medulloblastoma.	to document the incidence and outcome of patients with microscopic local leptomeningeal invasion at presentation, children with medulloblastoma were reviewed.	1997	9308951
anterior thoracic corpectomy without sternotomy: a strategy for malignant disease of the upper thoracic spine.	with increasing frequency, spine surgeons are being asked to provide decompression and stabilization in patients with spinal metastases. while no region of the spine is easily treated, the upper thoracic spine is perhaps the least accessible. traditional approaches to this region involve either thoracotomy or at least limited sternotomy. the authors present an approach to anterior pathology of the upper thoracic spine that obviates the need for sternotomy.	1997	9309285
thyroid-thymus interaction on beta-adrenoceptor regulation during development and ageing.	up-regulation of brain cortex beta-adrenoceptors (beta ars) can be induced by very acute stimulation with a single injection of t4 or t3 in young balb/c-nu mice. we have also shown that this very rapid receptor increase can also be demonstrated in ageing animals when stimulated with t3 but not t4 injection. the aim of the present paper was to verify the capability of the thymus to reverse these impairments which we often observed in other experiments on old mice and young athymic nudes. the up-r ...	1997	9309416
effect of estrogen replacement therapy with or without concomitant nonsteroidal anti-inflammatory drugs on pulsatility and resistance index of uterine arteries in healthy postmenopausal women.	this study assesses the existence of a prostaglandin-mediated effect of estrogen on uterine arteries.	1997	9309457
[treatment outcome of radical cystectomy during a 10-year period (june 1984-september 1994) at department of urology, fukui medical school].	the treatment outcome was evaluated in 63 patients with bladder cancer more than 2 years after cystectomy. cisplatin-based combination chemotherapies were given to 40 patients after the operation as an adjuvant therapy and two patients with t4 or n2 bladder cancer received chemotherapy before the operation as a neoadjuvant therapy. the extent of infiltration of the removed tumors was pta in 5, pt1 in 18, pt2 in 8, pt3a in 6, pt3b in 17, and pt4 in 9 cases. regional lymph node metastases were pre ...	1997	9310788
bcl-2 transgene expression promotes survival and reduces proliferation of cd3-cd4-cd8- t cell progenitors.	proliferative expansion and apoptotic cell death play prominent roles in t cell development. the molecular control of cell cycle progression and apoptosis appear to be inter-connected since the bcl-2 protein can inhibit apoptosis and slow cell cycle progression in cortical thymocytes and mature t cells, particularly during the transition from the quiescent state into the cell cycle. here the impact of bcl-2 transgene expression on cd3-cd4-cd8- t cell progenitors was assessed. bcl-2 enhanced the ...	1997	9310832
cell proliferation as an independent predictor of survival for patients with advanced nasopharyngeal carcinoma.	commonly used clinical and pathologic criteria are often of limited value in predicting the outcome of patients with undifferentiated nasopharyngeal carcinoma, and new parameters related to the biology of growth of neoplastic cells are still required for better definition of the aggressiveness of these tumors. the prognostic significance of dna ploidy, measured by image cytometry on isolated cells, and of the mitotic index, proliferating cell nuclear antigen, and p53 protein, all measured by ima ...	1997	9310951
interaction of the antitumour antibiotic streptonigrin with dna and oligonucleotides.	the interaction of the aminoquinone antitumour antibiotic streptonigrin with plasmid dna, calf thymus dna and oligonucleotides, in the presence and absence of metal ions, has been studied using circular dichroism, nmr spectroscopy and gel electrophoresis experiments. in the absence of metal ions, streptonigrin does not interact with dna. incubation of the two enantiomers of streptonigrin with calf thymus dna, in the presence of excess zinc(ii), showed no evidence of selective interaction of the ...	1997	9311555
thyroid hormone improves function and ca2+ handling in pressure overload hypertrophy. association with increased sarcoplasmic reticulum ca2+-atpase and alpha-myosin heavy chain in rat hearts.	we asked whether thyroid hormone (t4) would improve heart function in left ventricular hypertrophy (lvh) induced by pressure overload (aortic banding). after banding for 10-22 wk, rats were treated with t4 or saline for 10-14 d. isovolumic lv pressure and cytosolic [ca2+] (indo-1) were assessed in perfused hearts. sarcoplasmic reticulum ca2+-atpase (serca), phospholamban, and alpha- and beta-myosin heavy chain (mhc) proteins were assayed in homogenates of myocytes isolated from the same hearts. ...	1997	9312172
[prognostic value of lymphatic metastasis in bladder cancer].	the presence of lymph node metastasis in bladder cancer is considered to be a sign of poor prognosis. the present study was performed to analyze the influence of the number of positive lymph nodes on survival and the therapeutic value of lymphadenectomy in pn+patients.	1997	9313045
structure-activity relationship of a series of c-terminus modified aminoalkyl, diaminoalkyl- and anilino-containing analogues of the benzoic acid mustard distamycin derivative tallimustine: synthesis, dna binding and cytotoxicity studies.	as part of our investigations into the design of more cytotoxic analogues of the experimental anticancer drug tallimustine, 1, c-terminus modified aminoalkyl-, 2a-c, diaminoalkyl-, 3, and anilino-containing, 4, derivatives have been synthesized. compounds 2a-c differ by 2, 3, or 4 methylene units in the c-terminus, respectively. results from an ethidium displacement study on poly(da-dt), poly(dg-dc), calf thymus dna and t4 coliphage dna showed that compounds 2-4 interact in the minor groove of t ...	1997	9313856
thyroid hormones in the pathogenesis of lung hypoplasia and immaturity induced in fetal rats by prenatal exposure to nitrofen.	nitrofen is believed to act on prenatally exposed fetuses by changing maternal or fetal thyroid hormone physiology. the aim of this study was to determine whether the amounts of circulating and lung tissue t3 and t4 are decreased in rat fetuses with nitrofen-induced pulmonary hypoplasia and diaphragmatic hernia.	1997	9314246
thyroid peroxidase autoantibodies predict poor metabolic control and need for thyroid treatment in pregnant iddm women.	to study whether the presence of antithyroid peroxidase antibodies (tpo-abs) before gestation in iddm affects thyroid function and metabolic control during pregnancy and early postpartum as well as neonatal outcome.	1997	9314628
do cell kinetics have prognostic and/or predictive value in oesophageal cancer treated by surgery?	tumour cell kinetics could help in predicting the optimal duration of treatment for the individual patient. in order to assess the importance of cell kinetics in oesophageal cancer, 63 patients with cancer of the oesophagus and/or gastro-oesophageal junction were studied. seven patients had t1 tumours, six t2, 47 t3, and three t4. twenty patients had no pathological nodes, while 43 patients had node-positive disease. thirty-one patients had squamous cell carcinoma, 31 patients suffered from aden ...	1997	9315055
the effect of olpadronate in ovariectomized thyroxine-treated rats.	hyperthyroidism increases bone turnover and induces bone loss. this study examines the effect of thyroid hormone excess on two biochemical markers of bone turnover (hydroxyproline and bone alkaline phosphatase) as well as on bone mineral content (bmc) and bone mineral density (bmd). the possible protective role of dimethyl-apd (olpadronate, olp), on both suppression of bone turnover and bone mineral loss in ovariectomized (ovx) rats, was also studied. female sprague-dawley rats, were assigned to ...	1997	9315336
adrenal vasoactive intestinal peptide participates in neonatal corticosteroid production in the rat.	neonatal rats (3-14 days old) exhibit a period of adrenal hyporesponsiveness characterized by blunted corticosterone (b) responses to stress and reduced adrenal sensitivity to adrenocorticotropic hormone (acth). several adrenomedullary peptidergic systems like vasoactive intestinal peptide (vip) are postulated to influence cortical function. vip is known to stimulate corticosterone secretion in vitro and to be released from the adrenal medulla following splanchnic nerve stimulation. here, we tes ...	1997	9321900
the direct vasomotor effect of thyroid hormones on rat skeletal muscle resistance arteries.	the present study examines the hypothesis that the hormones have direct vasodilatory effects and attempts to determine whether the effects are endothelium-dependent. rat skeletal muscle resistance arteries of approximately 100 microns were dissected, and vessel diameter changes were monitored using a videodetection system. after equilibration at 37 degrees c, each vessel was preconstricted with the thromboxane analog u46619 1 microm, and the percentage of dilation was measured after exposure to ...	1997	9322448
alteration of platelet aggregation in patients with thyroid disorders.	to determine whether graves' disease or primary hypothyroidism influence platelet function, we evaluated platelet aggregation in the platelet-rich plasma (prp) from such patients. platelet aggregation induced by adenosine diphosphate (adp) in blood obtained from patients with untreated graves' disease was significantly lower than normal, whereas that in patients with untreated primary hypothyroidism was relatively increased. the magnitude of platelet aggregation induced by collagen in both group ...	1997	9322793
effect of 1 alpha-hydroxyvitamin d3 on serum levels of thyroid hormones in hyperthyroid patients with untreated graves' disease.	we performed a randomized, open prospective study to determine the effect of 1 alpha-hydroxyvitamin d3 [1 alpha (oh)d3] on hyperthyroidism in patients with untreated graves' disease. at the time of entry into the study, 30 patients were randomly assigned to receive a daily dose of 30 mg methimazole (mmi) (group a, n = 15) or the same dose of mmi supplemented with 1.5 micrograms 1 alpha (oh)d3 (group b, n = 15). these treatment regimens were continued for 24 weeks, and physicians were allowed to ...	1997	9322804
thyroid hormones influence serum leptin concentrations in the rat.	leptin, the product of the ob gene, is secreted by adipocytes and has been shown to decrease appetite and increase energy expenditure. leptin mrna in adipocytes correlates with body wt, and serum leptin levels correlate with body fat. alterations in thyroid status are frequently associated with changes in body wt. to evaluate the possible influence of thyroid status on the leptin system, we have measured serum leptin concentrations in thyroidectomized rats infused either with placebo, or with di ...	1997	9322968
[rational support of combined operations in locally disseminated stomach cancer].	an analysis of results of 315 combined operations for gastric cancer with the t3 and t4 degrees of invasion has shown that the rational multicomponent surgical, anesthesiological and therapeutic maintenance of surgical interventions for gastric cancer is the main factor responsible for the outcome of the treatment. the proposed scheme of the multicomponent rational maintenance of the combined surgical interventions allowed to reduce frequency of cardiopulmonary complications to 18.2%, pyoseptic ...	1997	9324838
interactions of the bacteriophage t4 gene 59 protein with single-stranded polynucleotides: binding parameters and ion effects.	the gene 59 protein (gp59) of bacteriophage t4 is an important accessory protein of the phage-encoded replicative dna helicase, gp41. the properties of this 26 kda protein include selective binding to ssdna, and specific interactions with both gp41 and gp32, the t4-encoded ssdna- binding protein. gp59 stimulates many of the dna-dependent activities of the gp41 enzyme by promoting its assembly onto gp32-ssdna complexes. direct interactions between gp59 and gp32-ssdna complexes are essential for h ...	1997	9325092
a phage t4 site-specific endonuclease, sege, is responsible for a non-reciprocal genetic exchange between t-even-related phages.	the bacteriophage t4 sege gene encoding site-specific endonuclease lies between the hoc.1 and uvsw genes. the similar region of t-even-related phage rb30 lacks the sege gene. here we demonstrate that the phage t4 sege gene is inherited preferably by progeny of mixed infection with rb30. the preferred inheritance of the sege gene depends on its own expression and is based on a non-reciprocal homologous recombination event providing the transfer of the gene from the sege-containing to the sege-lac ...	1997	9326373
supraglottic laryngectomy--series report and analysis of results.	between october 1987 and october 1993, 92 patients with squamous cell carcinoma of the supraglottis were treated by supraglottic laryngectomy and neck dissection in our department. there were 33 t1, 46 t2, six t3 and seven t4 cases. all patients with n+ necks and t3 or t4 tumours received post-operative radiotherapy (5,000-6,500 cgy). the patients were followed for a minimum of 36 months or until death. the incidence of local recurrence was 7.6 per cent. neck recurrence was observed in 13 per ce ...	1997	9327010
tumor control of locally advanced prostate cancer following combined estramustine, vinblastine, and radiation therapy.	a prospective phase ii study was carried out to determine whether estramustine phosphate (emp) plus vinblastine (vbl) in combination with radiotherapy (rt) would improve the control of locally advanced prostate cancer. the rationale for combining emp plus vbl with rt was based on the clinical and radiobiological data that emp plus vbl acted as an excellent radiation sensitizer in cultured human prostatic carcinoma cells with the property of tissue selectivity. the combined emp and vbl were well ...	1997	9327153
prognostic impact of splenectomy on gastric cancer: results of the korean gastric cancer study group.	the relation between splenectomy and survival time after curative total gastrectomy for gastric cancer was reviewed retrospectively on 492 patients treated at nine hospitals between 1989 and 1993. altogether 260 patients underwent splenectomy, and 232 patients did not. a univariate analysis revealed that the survival time of patients with splenectomy was significantly less than those without splenectomy (p = 0.0265). in a subgroup of our patients stratified to adjust for the stage of disease, th ...	1997	9327675
expression of differential nitric oxide synthase isoforms in human normal gastric mucosa and gastric cancer tissue.	the present study investigated the expression and distribution of three isoforms of nitric oxide synthase (nos) in different anatomical regions of the human stomach and in gastric neoplastic tissues by immunohistochemistry using specific antibodies. intracellular localization of individual isoenzymes of nos was detected in normal gastric mucosa. gastric cancer tissues had a marked reduction of all three nos isoforms expression. the expression of the endothelial nos, neuronal nos and inducible no ...	1997	9328184
purification, molecular cloning, and functional expression of the human nicodinamide-adenine dinucleotide phosphate-regulated thyroid hormone-binding protein.	the kidney and several other thyroid hormone-responsive tissues contain a nadp-regulated thyroid hormone (th)-binding protein (thbp), with an apparent molecular mass of 36 kda on sds-page, responsible for most of the intracellular high-affinity t3 and t4 binding. thbp was purified to homogeneity from human kidney cytosol and used to generate proteolytic peptides. microsequencing of four peptides revealed identity to amino acid sequences deduced from a human cdna homolog to a cdna encoding kangar ...	1997	9328354
regular exercise and the age-related decline in resting metabolic rate in women.	a low resting metabolic rate (rmr) is a risk factor for future weight gain. we tested the hypothesis that the age-related decline in rmr in sedentary women is not observed in women who exercise regularly. sixty-five healthy, weight-stable women, aged 21-35 or 50-72 yr, were studied: 12 premenopausal and 15 postmenopausal sedentary women, 13 premenopausal and 15 postmenopausal distance runners, and 10 endurance-trained postmenopausal swimmers. rmr was measured by indirect calorimetry (ventilated ...	1997	9329340
a novel missense mutation in codon 218 of the albumin gene in a distinct phenotype of familial dysalbuminemic hyperthyroxinemia in a japanese kindred.	familial dysalbuminemic hyperthyroxinemia (fdh) is the most common cause of inherited euthyroid hyperthyroxinemia in caucasians. to our knowledge, no such documentation on asians exists. six of 8 members of a 3-generation japanese family were found by us to carry the fdh phenotype. serum total t4 levels ranged from 1763.2-2741.3 nmol/l (normal range, 65.6-164.7), serum total t3 levels ranged from 2.73-5.62 nmol/l (normal range, 1.47-2.95), and rt3 levels ranged from 1.08-2.52 nmol/l (normal rang ...	1997	9329347
thyroid function in rubinstein-taybi syndrome.	rubinstein-taybi syndrome (rts) is a genetic syndrome characterized by broad thumbs and halluces, growth retardation, mental retardation, and craniofacial abnormalities. this condition recently was found to be caused by mutations in the gene encoding camp response element-binding protein (creb)-binding protein. as creb-binding protein has been shown to be a critical coactivator for thyroid hormone receptors, it is plausible that rts would be characterized by thyroid hormone resistance. in fact, ...	1997	9329350
liposomal thyroxine: a noninvasive model for transplacental fetal therapy.	drugs that cross the placenta sparingly are currently given directly to the fetus by invasive procedures. we investigated whether anionic small unilamellar (suv) liposomes of different lipid compositions enhanced the transfer and uptake of t4 in an in vitro model of perfused human term placenta. t4-encapsulated anionic liposomes were prepared using lecithin (f-suv) or distearoyl phosphatidylcholine (s-suv) with cholesterol and dicetylcholine. the size distribution, encapsulation efficiency, and ...	1997	9329352
clinical and biochemical features of muscle dysfunction in subclinical hypothyroidism.	alterations in muscle structure and function have been reported in overt hypothyroidism, with decreased activity of enzymes involved in anaerobic and oxidative glucose metabolism. to test whether similar changes in muscle energy metabolism are present in subclinical hypothyroidism (sht), we studied 12 patients with sht who complained of mild neuromuscular symptoms. the control group included 10 sex- and age-matched healthy volunteers. skeletal muscle lactate and pyruvate production were determin ...	1997	9329360
both hypothyroidism and hyperthyroidism enhance low density lipoprotein oxidation.	hypothyroidism is frequently associated with hypercholesterolemia and an increased risk for atherosclerosis, whereas hyperthyroidism is known to precipitate angina or myocardial infarction in patients with underlying coronary heart disease. we have shown previously that l-t4 functions as an antioxidant in vitro and inhibits low density lipoprotein (ldl) oxidation in a dose-dependent fashion. the present study was designed to evaluate the changes in ldl oxidation in subjects with hypothyroidism a ...	1997	9329379
topical iodine-containing antiseptics and subclinical hypothyroidism in preterm infants.	the influence of topical iodine-containing antiseptics on thyroid function test results of premature infants was determined in two separate studies. thyroxine and thyrotropin levels were measured on blood-spotted filter paper. samples were obtained from 128 premature infants on their tenth day of life; the infants were treated in two neonatal intensive care units. both units used similar treatment protocols; however, one routinely used topical iodinated antiseptic agents (n = 73), whereas the ot ...	1997	9329422
serum free thyroxine concentration is not reduced in premature infants with respiratory distress syndrome.	we used improved methods of assay to determine whether pituitary-thyroid function is altered in premature infants with respiratory distress syndrome (rds) during the first week of postnatal life.	1997	9329438
combined-modality therapy for bladder cancer.	radical cystectomy remains standard management for patients with locally advanced t2 through t4, n0, m0 transitional cell carcinoma of the urinary bladder. although radical cystectomy results in excellent local control, 50% or more of patients relapse. studies have shown that multidrug cisplatin (platinol)-based chemotherapy prolongs disease-free survival in 10% to 15% of cases and is superior to single-agent cisplatin. these studies led to the application of these regimens in conjunction with s ...	1997	9330404
developmental changes in regulation of the na+, k(+)-atpase alpha 3 isoform by thyroid hormone in ferret heart.	ferret heart expresses the alpha 1- as well as the alpha 3-isoform of the na+, k(+)-atpase. we have shown previously that the alpha 3 isoform is differentially upregulated during postnatal cardiac development and that in adult ferrets expression of alpha 3 is not responsive to regulation by thyroid hormone (th). since developmental-stage dependent effects of th have been reported previously, the present study examined whether effects of th on expression of the na+, k(+)-atpase isoforms in ferret ...	1997	9332453
proposal for improved staging criteria for carcinoma of the esophagus and cardia.	current staging for carcinoma of the esophagus and cardia remains imprecise. in an effort to improve on presently accepted staging criteria, new and improved criteria were sought.	1997	9332912
[significance of atrial signal-averaged electrocardiogram analysis in diagnosis of paroxysmal atrial fibrillation in patients with mitral valve prolapse syndrome].	the aim of this study was to assess the clinical usage of recording time-domain parameters of atrial signal-averaged electrocardiogram (asaecg) in diagnosis of paroxysmal atrial fibrillation (paf) of patients with mitral valve prolapse (mvp). 85 patients with mvp recognized by echocardiography were divided into two groups: group i (mvp-pag/+/) 41 pts (15 male and 26 female) mean age 37.1 +/- 8.9 with previously electrocardiographically documented episode of paf, group ii (mvp-paf/-/) 44 pts (20 ...	1997	9333769
horizontovertical laryngectomy for supraglottic carcinoma.	recent decades have seen the expansion of indications for partial laryngectomy. this study focused on the application of horizontovertical laryngectomy for t2 and selected t3 and t4 supraglottic carcinomas. an osteomuscular flap was designed to reconstruct the laryngeal defect to preserve all the essential laryngeal functions. seventy-six patients treated between november 1979 and october 1990 were reviewed retrospectively. all but two were followed up for at least 5 years. there were 11 cases o ...	1997	9334778
photodynamic therapy using mthpc for malignant disease in the oral cavity.	photodynamic therapy (pdt) produces local tumor necrosis, on activation of a previously administered sensitizer with non-thermal light of an appropriate wavelength. it is attractive for treating tumors of the mouth as tissue healing is particularly good. we describe the use of the photosensitizing agent meta tetrahydroxyphenyl chlorin (mthpc, foscan) for pdt of oral cancer, including patients with field cancerization. nineteen patients with histologically confirmed oral cancer (8 with field chan ...	1997	9334805
basal muscarinic activity does not impede beta-adrenergic activation in rabbit hearts in controls or thyroxine-induced cardiac hypertrophy.	we tested the hypothesis that basal myocardial muscarinic receptor activity acts as a "brake" on beta-adrenergic activation and that this effect would be greater in hearts subjected to thyroxine (t4)-induced (0.5 mg/kg for 16 days) hypertrophy due to an increase in muscarinic receptor density. twenty control and 20 t4-treated open-chest anesthetized new zealand white rabbits were given isoproterenol (0.5 microg/kg/min, 10 min i.v.) and/or atropine (3 mg/kg bolus). coronary blood flow (radioactiv ...	1997	9335397
[predictive value of the measurement of iodothyronines in the prognosis of patients with severe nonthyroidal illness].	in order to find prognostic parameters in patients with severe diseases, we analyzed sequentially the levels of thyroid hormones.	1997	9336046
influence of tumor position on accuracy of endorectal ultrasound staging.	endorectal ultrasound is a well-established method of preoperative staging of rectal neoplastic lesions.	1997	9336113
the use of combined radiation therapy and hormonal therapy in the management of lymph node-positive prostate cancer.	to determine the rate of tumor response and patterns of relapse following combined hormonal-radiation therapy of adenocarcinoma of the prostate and to measure the survival in a group of men with tumor metastatic to pelvic lymph nodes.	1997	9336149
prokaryotic 5'-3' exonucleases share a common core structure with gamma-delta resolvase.	the three dimensional crystal structure of t5 5'-3' exonuclease was compared with that of two other members of the 5'-3' exonuclease family: t4 ribonuclease h and the n-terminal domain of thermus aquaticus dna polymerase i. though these structures were largely similar, some regions of these enzymes show evidence of significant molecular flexibility. previous sequence analysis had suggested the existence of a helix-hairpin-helix motif in t5 exonuclease, but a distinct, though related structure is ...	1997	9336450
interaction of the phage t4 dam dna-[n6-adenine] methyltransferase with oligonucleotides containing native or modified (defective) recognition sites.	the dna-[n 6-adenine]-methyltransferase (dam mtase) of phage t4 catalyzes methyl group transfer from s-adenosyl-l-methionine (adomet) to the n6-position of adenine in the palindromic sequence, gatc. we have used a gel shift assay to monitor complex formation between t4 dam and various synthetic duplex oligonucleotides, either native or modified/defective. the results are summarized as follows. (i) t4 dam bound with approximately 100-fold higher affinity to a 20mer specific (gatc-containing) dupl ...	1997	9336474
evaluating the energetics of empty cavities and internal mutations in proteins.	the energetics of cavity formation in proteins is evaluated with two different approaches and results are analyzed and compared to experimental data. in the first approach, free energy of cavity formation is extracted by rms fitting from the distribution of numbers of cavities, n, with different volumes, vcav, in 80 high-resolution protein structures. it is assumed that the distribution of number of cavities according to their volume follows the boltzmann law, n(vcav) = exp [(-a.vcav-b)/kt], or ...	1997	9336837
interaction of the dna topoisomerase ii catalytic inhibitor meso-2,3-bis(3,5-dioxopiperazine-1-yl)butane (icrf-193), a bisdioxopiperazine derivative, with the conserved region(s) of eukaryotic but not prokaryotic enzyme.	icrf-193 [meso-2,3-bis(3,5-dioxopiperazine-1-yl)butane], a bisdioxopiperazine compound, has been shown to be a catalytic inhibitor of dna topoisomerase ii by stabilizing the enzyme in the form of a closed "protein clamp," an intermediate form in the catalytic cycle (roca et al., proc natl acad sci usa 91: 1781-1785, 1994). in view of its usefulness as a probe in the functional analysis of the enzyme, we tried further to define the domain(s) of the enzyme interacting with the drug by examining it ...	1997	9337070
studies on angiogenesis intensity in lung cancer in aspect of its correlation with histological type of tumor and clinical stage.	angiogenesis intensity in lung cancer, in compliance with histological types, tumor differentiation and different clinical stage of disease, was evaluated. the group of 65 patients, 34-73 years old (average 58), who have been operated, were examined. microvessels were highlighted by immunohistochemical method staining of endothelial cells for factor viii-von willebrand. microvessel and single endothelial cell count per 1 mm2 in each section was determined using light microscope, synchronized wit ...	1997	9337543
sleep and quantitative eeg in patients with progressive supranuclear palsy.	sleep architecture and quantitative eeg from wakefulness and rem sleep were studied in six patients (mean age, 70.5 years) with progressive supranuclear palsy (psp) and compared with that of six control subjects (mean age, 69.8 years). particular attention was given to quantifying rem sleep variables because of the known psp-associated degeneration of the pedunculopontine tegmentum (ppt)--a critical structure in rem sleep generation. patients with psp had a shorter total sleep time, a lower slee ...	1997	9339679
the role of base flipping in damage recognition and catalysis by t4 endonuclease v.	the process of moving a dna base extrahelical (base flipping) has been shown in the co-crystal structure of a uv-induced pyrimidine dimer-specific glycosylase, t4 endonuclease v, with its substrate dna. compared with other enzymes known to use base flipping, endonuclease v is unique in that it moves the base opposite the target site extrahelical, rather than moving the target base itself. utilizing substrate analogs and catalytically inactive mutants of t4 endonuclease v, this study investigates ...	1997	9341165
the interaction between the asia protein of bacteriophage t4 and the sigma70 subunit of escherichia coli rna polymerase.	the asia protein of bacteriophage t4 binds to the sigma70 subunit of escherichia coli rna polymerase and plays a dual regulatory role during t4 development: (i) inhibition of host and phage early transcription, and (ii) coactivation of phage middle-mode transcription, which also requires the t4 dna binding transcriptional activator, mota. we report that the interaction between asia and sigma70 occurs with a 1:1 stoichiometry. when preincubated with rna polymerase, asia is a potent inhibitor of o ...	1997	9341196
potassium bromide and the thyroid gland of the rat: morphology and immunohistochemistry, ria and inaa analysis.	the increasing environmental concentration of bromine has resulted in attempts to obtain information on its possibly deleterious effect on humans, particularly on a major target organ of this halogen i.e. the thyroid gland. in order to establish the morphological and functional effects of bromine on the thyroid, we have performed experiments on male rats which, in addition to a standard diet with an estimated iodine/bromine content, were fed for periods of 16 and 66 days with the small quantitie ...	1997	9341949
modeling protein stability: a theoretical analysis of the stability of t4 lysozyme mutants.	free energy calculations were conducted to determine the relative stability of the unnatural amino acid mutants of t4 lysozyme norvaline (nvl) and o-methyl-serine (mse) and of alanine at residue 133, which is leucine in the native sequence. these calculations were performed both to assess the validity of the methodology and to gain a better understanding of the forces which contribute to protein stability. peptides of different length were used to model the denatured state. restraints were emplo ...	1997	9342145
repair pattern in the beta-globin gene cluster of human fibroblasts after ultraviolet irradiation.	we have developed a novel technique to determine repair of structurally different dna lesions. it was used to address the question of whether dna repair in the absence of transcription occurs in a uniformly random manner or with preferences for certain regions. human fibroblasts were exposed to ultraviolet light (3-10 j/m2) and treated with 7.5 mm hydroxyurea to inhibit replicative dna synthesis. during the first hours after irradiation cells were treated with 5-bromodeoxyuridine to label the re ...	1997	9342216
increased prevalence of thyroid autoantibodies and subclinical thyroid failure in relatives of patients with overt endocrine disease-associated diabetes but not type 1 diabetes alone.	the purpose of this study was to determine the prevalence of thyroperoxidase (tpo) and thyroglobulin (tg) antibodies, using a sensitive and specific radioimmunoassay method in a large cohort of 254 first-degree relatives of type 1 diabetic patients with or without other autoimmune endocrinopathy, and to evaluate the predictive value of thyroid antibodies for impaired thyroid function in these groups. tpo and tg antibodies were found at similar frequencies (12%) in the 254 relatives, and both ant ...	1997	9342543
immunoassay for thyroxine (t4) in serum using capillary electrophoresis and micromachined devices.	as part of our ongoing effort to develop electrophoretic assay technology for clinical diagnostics, we describe a competitive immunoassay for the determination of serum thyroxine (t4) based on electrophoresis and laser induced fluorescence (lif). measurements of total t4 are useful for the clinical evaluation of thyroid function. a fluorescein thyroxine conjugate was utilized in conjunction with a polyclonal antibody preparation as assay reagents. capillary electrophoresis (ce) conditions tolera ...	1997	9342667
excessive daytime sleepiness associated with idiopathic alterations of consciousness.	sleep/wake patterns were recorded by continuous 24-hour ambulatory polysomnography in 339 patients, who had episodes of altered consciousness. patients were recorded while they were outside the hospital. from a seven-channel montage of electrodes affixed below the hairline, sleep polygraphic eeg was easily read from t3-t4, eog from f2-f8 and emg from t3-t6. sleep was staged by analysis of aural signals on 60 times real time playback, augmented by continuous visual display and selected frozen fra ...	1997	9343713
carcinoma of the tonsil: prognostic factors.	this study was conducted to provide a review of the prognostic factors of tonsillar carcinoma.	1997	9343766
evaluation of a training program for persons with sci paraplegia using the parastep 1 ambulation system: part 1. ambulation performance and anthropometric measures.	to describe performance parameters and effects on anthropometric measures in spinal cord injured subjects training with the parastep 1 system.	1997	9344294
evaluation of a training program for persons with sci paraplegia using the parastep 1 ambulation system: part 2. effects on physiological responses to peak arm ergometry.	to examine the task-nonspecific effects of functional neuromuscular stimulation (fns)-assisted ambulation training on the physiological responses of persons with paraplegia to upper extremity exercise challenge.	1997	9344295
primary hormonogenic sites as conserved autoepitopes on thyroglobulin in murine autoimmune thyroiditis: role of mhc class ii.	a few synthetic peptides corresponding to amino acid sequences on human thyroglobulin (tg) have been reported to induce moderate thyroiditis or activate mouse tg (mtg)-primed t cells to transfer thyroiditis in mice susceptible to experimental autoimmune thyroiditis. using three pairs of 12-mer peptides (1-12, 2549-2560, 2559-2570), with thyroxine (t4) or noniodinated thyronine (t0) at the conserved, hormonogenic site 5, 2553, or 2567 respectively, we reported that iodination was not required for ...	1997	9344702
subchronic/chronic toxicity of 1,2,3,4,6,7,8-heptachlorodibenzo-p-dioxin (hpcdd) in rats. part ii. biochemical effects.	groups of 20 male and 20 female rats were given five different oral doses of 1,2,3,4,6,7,8-heptachlorodibenzo-p-dioxin (hpcdd) or one dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) divided into four daily loading doses and six biweekly maintenance doses. the dosing period was 13 weeks, after which half of the rats were necropsied and the rest assigned to an off-dose period of another 13 weeks. at the end of the dosing period, liver ethoxyresorufin o-deethylase (erod) activity was dose-depend ...	1997	9344889
induction intraarterial chemotherapy for t4 breast cancer through an implantable port-catheter system.	eleven patients with t4 breast cancer received induction intraarterial chemotherapy (iact) as the first step in multidisciplinary therapy. the iact agents (epirubicin and mitomycin c), were delivered weekly in the outpatient department by bolus injection through an implantable port-catheter system. a modified technique of port-catheter system implantation was used. the precise localization of the catheter was dually confirmed by angiography and dye test. the effectiveness of the treatment was ev ...	1997	9345335
the influence of hyperthyroidism on vasoconstrictor and vasodilator responses in isolated coronary and renal resistance arteries.	the influence of hyperthyroidism on the functional vascular responsiveness of isolated coronary and renal resistance vessels was investigated. hyperthyroidism was established by feeding rats for 1 and 4 weeks with 5 mg/kg l-thyroxine (t4)-containing rat chow. preparations of either coronary or renal resistance vessels were mounted in an isometric wire myograph. subsequently, concentration-effect curves were determined for the effects of 5-hydroxytryptamine (5-ht), 9,11-dideoxy-11alpha,9alpha-epo ...	1997	9346400
small doses of triiodothyronine can change some risk factors associated with abdominal obesity.	to elucidate whether the administration of small doses of triidothyronine (t3) can increase concentrations of sex hormone binding globulin (shbg) in obese women with different types of obesity and to evaluate the potential metabolic benefits of such treatment.	1997	9347411
oxygen uptake and heart rate responses during arm vs combined arm/electrically stimulated leg exercise in people with paraplegia.	the purpose of this study was to compare the oxygen uptake and heart rate responses during submaximal arm cranking to combined arm cranking + electrical stimulation (es)-induced leg cycling in individuals with spinal cord injury (sci). seven subjects with paraplegia (t4-t12) performed combined arm and leg cycling exercise for 5 min, followed by arm cranking alone at the same power output for a further 5 min. during both exercise conditions, steady state oxygen consumption (vo2), carbon dioxide o ...	1997	9347597
is the brain death related endocrine dysfunction an indication for hormonal substitution therapy in the early period ?	experimental studies in animals have suggested that brain death (bd) -- related endocrinological dysregulations lead to a significant depression of cardiac pump and muscle function, however, the discussion about the relative extent of this influence remains controversal. the aim of the present study was to assess in an open chest animal model the short time course (5 hours) of hormonal (epinephrine, norepinephrine, t3, t4, acth, cortisol, insuline) and metabolic (glucose, lactate) changes in 10 ...	1997	9348271
postoperative adjuvant chemotherapy with mitomycin c and uft for rectal cancer.	to evaluate the significance of postoperative adjuvant chemotherapy using mitomycin c (mmc) and uft (tegafur and uracil) in combination, the japanese foundation for multidisciplinary treatment of cancer conducted a prospective randomized controlled trial with 834 patients who had undergone curative resection for rectal cancer (t3 or t4 tumors and/or n1, n2, or n3 disease). the patients were randomly allocated to a treatment group (mmc/uft, 416 patients) and a control group (surgery only, 418 pat ...	1997	9348566
preoperative combined oral uft plus leucovorin and radiation therapy for rectal cancer.	several trials performed in the united states and europe have demonstrated the efficacy of uft (uracil and tegafur in a 4:1 molar combination) with oral leucovorin in the treatment of several tumor types, but particularly for advanced colorectal cancer. phase iii studies are under way in the united states to determine whether the combination of uft with oral leucovorin is as effective as standard treatment, not only in the advanced setting but also in the adjuvant arena as well. this study is an ...	1997	9348571
levothyroxine suppressive therapy is partially effective in treating patients with benign, solid thyroid nodules and multinodular goiters.	we prospectively evaluated the effect of thyrotropin (tsh)-suppressive therapy with levothyroxine (lt4) on the size of a benign, solitary, solid nodule and multinodular goiter in a relatively low iodine intake area. in this study, 101 euthyroid subjects with a benign, solitary, predominantly solid nodule (n = 54) confirmed by biopsy or multinodular goiter (n = 47) received 200 microg of levothyroxine daily as a single morning tablet for 12 months. thirty-five receiving no therapy were considered ...	1997	9349571
incidence and clinical characteristics of thyroid carcinoma after iodine prophylaxis in an endemic goiter country.	iodized salt prophylaxis has been performed in austria since 1963. through this approach, mean urinary iodine excretion has been normalized to 144+/-23.5 microg/g creatinine per day. thus tyrol is no longer an endemic goiter area. we have analyzed the impact of iodized salt prophylaxis on thyroid cancer (tc) comparing data from the early 1960s with those corresponding to the period 1986 to 1995, when iodine supply was normalized. the study included 439 patients from tyrol and southern tyrol. the ...	1997	9349576
autoimmune hyperthyroidism in prepubertal children and adolescents: comparison of clinical and biochemical features at diagnosis and responses to medical therapy.	we explored our clinical impression that young children with autoimmune hyperthyroidism are more thyrotoxic at presentation and require a longer course of medical therapy than do adolescents to achieve remission. a retrospective chart review of clinical and biochemical data at presentation and response to therapy in 32 prepubertal (prepub) and 68 pubertal (pub) children and adolescents with autoimmune hyperthyroidism was undertaken. initial therapy included prophylthiouracil or methimazole in al ...	1997	9349579
thyroid hormones and cytogenetic outcomes in backpack sprayers using ethylenebis(dithiocarbamate) (ebdc) fungicides in mexico.	ethylenebis(dithiocarbamate) (ebdc) fungicides are used heavily in the united states. ebdcs (e.g., mancozeb, maneb) are metabolized to ethylene thiourea (etu). the epa classifies etu as a carcinogen, based on thyroid and other cancers in rodents, and has restricted the use of ebdcs, while requiring workers to use protective equipment. there are no data on the potential carcinogenicity of ebdcs in humans, and there is only one study on human genotoxicity. etu is known to cause decreases of thyrox ...	1997	9349837
evaluation of proliferating cell nuclear antigen (pcna) in supraglottic carcinoma.	it is widely accepted that tumor invasion and metastasis are the primary causes for the lethal clinical outcome of cancers. in recent years, attention has been drawn to pcna (proliferating cell nuclear antigen) to predict the prognosis of some cancers. in the present paper, we have studied anti-pcna antibody pc10 in supraglottic cancer by means of immunohistochemistry using paraffin-embedded sections, to demonstrate its clinical significance in this type of malignancy. twenty-two patients with s ...	1997	9350310
continuous spinal anaesthesia using a standard epidural set for extracorporeal shockwave lithotripsy.	continuous spinal anaesthesia (csa) offers considerable advantages over "single shot" spinal or epidural anaesthesia since it allows titration of anaesthesia using small doses of local anaesthetics (la). we evaluated the feasibility of csa using a standard epidural set for extracorporeal shockwave lithotripsy (eswl).	1997	9350361
the impact of thyroid autoimmunity in children and adolescents with down syndrome.	the extent to which autoimmunity contributes to thyroid dysfunction in individuals with down syndrome (ds) has not been clarified. in this study, we used the same highly sensitive method to detect both thyroid autoantibodies (thyroglobulin and thyroid peroxidase autoantibodies) in 70 children (32 m and 38 f) with ds, mean age 10.5 y (range 1-19 y). twenty-seven (39%) of the patients were found to have thyroid autoantibodies, the prevalence of antibody positivity increasing with age. of the 17 (2 ...	1997	9350885
prevalence of enuresis and other bladder symptoms in patients with active graves' disease.	to determine the prevalence of bladder symptoms in patients with graves' disease and assess their abnormalities on urodynamic studies.	1997	9352693
characterization of dna topoisomerase activity in two strains of mycoplasma fermentans and in mycoplasma pirum.	dna topoisomerases (topos) are essential enzymes that participate in many cellular processes involving dna. the presence of the dna-gyrase genes in various mycoplasmas has been reported elsewhere. however, the characterization of dna topo activity in mycoplasmas has not been previously undertaken. in this study, we characterized the topo activity in extracts of mycoplasma fermentans k7 and incognitus and in mycoplasma pirum, as well as in partially purified extract of m. fermentans k7. the topo ...	1997	9352909
display of a pora peptide from neisseria meningitidis on the bacteriophage t4 capsid surface.	the exterior of bacteriophage t4 capsid is coated with two outer capsid proteins, hoc (highly antigenic outer capsid protein; molecular mass, 40 kda) and soc (small outer capsid protein; molecular mass, 9 kda), at symmetrical positions on the icosahedron (160 copies of hoc and 960 copies of soc per capsid particle). both these proteins are nonessential for phage infectivity and viability and assemble onto the capsid surface after completion of capsid assembly. we developed a phage display system ...	1997	9353063
the 5'-exonuclease activity of bacteriophage t4 rnase h is stimulated by the t4 gene 32 single-stranded dna-binding protein, but its flap endonuclease is inhibited.	bacteriophage t4 rnase h is a 5'- to 3'-nuclease that has exonuclease activity on rna.dna and dna.dna duplexes and can remove the pentamer rna primers made by the t4 primase-helicase (hollingsworth, h. c., and nossal, n. g. (1991) j. biol. chem. 266, 1888-1897; hobbs, l. j., and nossal, n. g. (1996) j. bacteriol. 178, 6772-6777). here we show that this exonuclease degrades duplex dna nonprocessively, releasing a single oligonucleotide (nucleotides 1-4) with each interaction with the substrate. d ...	1997	9353314
identification of residues of t4 rnase h required for catalysis and dna binding.	bacteriophage t4 rnase h, which removes the rna primers that initiate lagging strand fragments, has a 5'- to 3'-exonuclease activity on dna.dna and rna.dna duplexes and an endonuclease activity on flap or forked dna structures (bhagwat, m., hobbs, l. j., and nossal, n. j. (1997) j. biol. chem. 272, 28523-28530). it is a member of the rad2 family of prokaryotic and eukaryotic replication and repair nucleases. the crystal structure of t4 rnase h, in the absence of dna, shows two mg2+ ions coordina ...	1997	9353315
hla-a2 antigen status predicts metastasis and response to immunotherapy in gastric cancer.	our previous studies have shown that hla-dr4 and -b52 antigens are associated with an increased risk of lymph node metastasis in patients with gastric cancer. we hypothesized that a putative hla antigen, correlated with a low risk of lymph node metastasis, may also be correlated with the response to anticancer therapy. the microcytotoxicity assay was used to examine 49 hla antigens of the a, b, c, dr, and dq loci, and the association between hla class i and ii antigen status and lymph node metas ...	1997	9353427
transrectal sonography in staging rectal carcinoma: the role of gray-scale, color-flow, and doppler imaging analysis.	the purpose of this study was to evaluate the efficacy of combining gray-scale sonography with color-flow imaging and pulsed doppler transrectal sonography in the staging of rectal carcinoma.	1997	9353436
efficient characterization of collective motions and interresidue correlations in proteins by low-resolution simulations.	a low-resolution model is used together with recently developed knowledge-based potentials for exploring the dynamics of proteins. configurations are generated using a monte carlo/metropolis scheme combined with a singular value decomposition technique (svd). the approach is shown to characterize the cooperative motions in good detail, at least 1 order of magnitude faster than atomic simulations. trajectories are partitioned into modes, and the slowest ones are analyzed to elucidate the dominant ...	1997	9354619
clinicopathological features of prostate cancer detected by transrectal ultrasonography-guided systematic six-sextant biopsy.	the objectives of this study were to compare the efficacy of 3 modalities (prostate-specific antigen (psa) assay, digital rectal examination (dre), and transrectal ultrasonography (trus)) in detecting prostate cancer which was pathologically confirmed by trus-guided systematic six-sextant biopsy, and to investigate the relationship between the number of positive cores and several clinicopathological parameters.	1997	9354949
the value of chest ct scan in the work-up of head and neck cancers.	the systemic work-up of patients with head and neck cancers includes a routine chest x-ray and panendoscopy. in patients with a high risk for head and neck cancers, such as heavy smokers and drinkers, a high incidence of second upper aerodigestive tract tumors is expected, in addition to the risk of metastatic spread from their primary neoplasm. in this study, we evaluated the contribution of a chest cat in patients with negative plain chest x-rays. twenty-four consecutive patients with head and ...	1997	9355023
the effect of fasting and refeeding on thyroid follicule structure and thyroid hormone levels in young and old rats.	in order to assess morphological and functional plasticity of the thyroid gland in aging the effects of fasting and refeeding on the thyroid morphology and thyroid hormone serum levels were compared with morphometry and ria in young and old rats. young (4 months) and old (24 months) male wistar rats were fasted for 40 h and sampled, or fasted and fed thereafter for 4 or 24 h. in control (fed) old animals the thyroid follicules were larger, the follicular epithelium was smaller and colloid resorp ...	1998	18653134