Publications

mast cells in squamous cell esophageal carcinoma and clinical parameters.	esophageal carcinoma is a malignancy with a poor prognosis and new treatment modalities must be sought. one possibility that has been tested in patients with malignant melanoma is treatment which aims towards boosting the immune system. in the present study, we investigated the role of mast cells in patients with esophageal carcinoma. the intention was to determine whether a higher number of mast cells is associated with better survival and may thus be a marker for future immunotherapeutic studi ...	2015	20181628
turnover of transmitter and synaptic vesicles at the frog neuromuscular junction.	curarized cutaneous pectoris nerve-muscle preparations from frogs were stimulated at 10/s or at 2/s for periods ranging from 20 min to 4 h. end plate potential were recorded intracellularly and used to estimate the quantity of transmitter secreted during the period of stimulation. at the ends of the periods of stimulation the preparations were either fixed for electron microscopy or treated with black widow spider venom to determine the quantities of transmitter remainind in the terminal. horser ...	2015	4348791
the detection of anti-nuclear factor using horseradish peroxidase.		2015	4568665
study of peroxidase mechanisms by pulse radiolysis. ii. reaction of horseradish peroxidase compound i with o2-.		2015	4407345
evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction.	when the nerves of isolated frog sartorius muscles were stimulated at 10 hz, synaptic vesicles in the motor nerve terminals became transiently depleted. this depletion apparently resulted from a redistribution rather than disappearance of synaptic vesicle membrane, since the total amount of membrane comprising these nerve terminals remained constant during stimulation. at 1 min of stimulation, the 30% depletion in synaptic vesicle membrane was nearly balanced by an increase in plasma membrane, s ...	2015	4348786
studies on horseradish peroxidase. 13. the kinetic effect of cyanide on the oxidation-reduction cycle.		2015	4706838
peroxidase activity and involvement in the oxidative stress response of roseobacter denitrificans truncated hemoglobin.	roseobacter denitrificans is a member of the widespread marine roseobacter genus. we report the first characterization of a truncated hemoglobin from r. denitrificans (rd. trhb) that was purified in the heme-bound form from heterologous expression of the protein in escherichia coli. rd. trhb exhibits predominantly alpha-helical secondary structure and absorbs light at 412, 538 and 572 nm. the phylogenetic classification suggests that rd. trhb falls into group ii trhbs, whereas sequence alignment ...	2015	25658318
preparation and preliminary application of monoclonal antibodies to the receptor binding region of clostridium difficile toxin b.	a previous nationwide chinese epidemiological study revealed through isolation of a‑b+ clostridium difficile strains, which produce toxin b (tcdb), but not toxin a tcda, that the strains are widespread and more frequent in east asian countries,. the development of a process capable of detecting tcdb is required in microbiological laboratories in order to facilitate the control of the a‑b+ c. difficile strains, however, no diagnostic reagents have been developed to date. the aim of the present st ...	2015	26459027
a recombinant antigen-based enzyme-linked immunosorbent assay (elisa) for lungworm detection in seals.	pinnipeds are frequently infected by the lungworms otostrongylus circumlitus and parafilaroides gymnurus (metastrongyloidea). infections are frequently associated with secondary bacterial bronchopneumonia and are often lethal. to date, a reliable lungworm diagnosis in individual seals is only possible during necropsy as examination of faeces collected from resting places does not allow assignment to individuals. therefore, a diagnostic tool for lungworm detection in living seals is desirable for ...	2015	26329933
accurate detection of adenylation domain functions in nonribosomal peptide synthetases by an enzyme-linked immunosorbent assay system using active site-directed probes for adenylation domains.	a significant gap exists between protein engineering and enzymes used for the biosynthesis of natural products, largely because there is a paucity of strategies that rapidly detect active-site phenotypes of the enzymes with desired activities. herein, we describe a proof-of-concept study of an enzyme-linked immunosorbent assay (elisa) system for the adenylation (a) domains in nonribosomal peptide synthetases (nrpss) using a combination of active site-directed probes coupled to a 5'-o-n-(aminoacy ...	2015	26474351
anti-inflammatory activity of horseradish (armoracia rusticana) root extracts in lps-stimulated macrophages.	horseradish (armoracia rusticana) is a perennial crop belonging to the brassicaceae family. horseradish root is used as a condiment due to its extremely pungent flavour, deriving from the high content of glucosinolates and their breakdown products such as isothiocyanates and other sulfur compounds. horseradish also has a long history in ethnomedicine. in this study the anti-inflammatory potential of three accessions of armoracia rusticana on lipopolysaccharide from e. coli treated j774a.1 murine ...	2015	26411988
investigating the effect of gallium curcumin and gallium diacetylcurcumin complexes on the structure, function and oxidative stability of the peroxidase enzyme and their anticancer and antibacterial activities.	curcumin has a wide spectrum of biological and pharmacological activities including anti-inflammatory, antioxidant, antiproliferative, antimicrobial and anticancer activities. complexation of curcumin with metals has gained attention in recent years for improvement of its stability. in this study, the effect of gallium curcumin and gallium diacetylcurcumin on the structure, function and oxidative stability of horseradish peroxidase (hrp) enzyme were evaluated by spectroscopic techniques. in addi ...	2015	26369539
strong protection against ricin challenge induced by a novel modified ricin a-chain protein in mouse model.	ricin toxin (rt) is an extremely potent toxin derived from the castor bean plant. as a possible bioterrorist weapon, it was categorized as a level b agent in international society. with the growing awareness and concerns of the "white powder incident" in recent years, it is indispensable to develop an effective countermeasure against rt intoxication. in this study we used site-directed mutagenesis and polymerase chain reaction (pcr) techniques to modify the gene of ricin a-chain (rta). as a resu ...	2015	26038805
ultra-high-throughput screening of an in vitro-synthesized horseradish peroxidase displayed on microbeads using cell sorter.	the c1a isoenzyme of horseradish peroxidase (hrp) is an industrially important heme-containing enzyme that utilizes hydrogen peroxide to oxidize a wide variety of inorganic and organic compounds for practical applications, including synthesis of fine chemicals, medical diagnostics, and bioremediation. to develop a ultra-high-throughput screening system for hrp, we successfully produced active hrp in an escherichia coli cell-free protein synthesis system, by adding disulfide bond isomerase dsbc a ...	2015	25993095
high-yield reactivation of anionic tobacco peroxidase overexpressed in escherichia coli.	anionic tobacco peroxidase (top) is extremely active in chemiluminescence reaction of luminol oxidation without addition of enhancers and more stable than horseradish peroxidase under antibody conjugation conditions. in addition, recombinant top (rtop) produced in escherichia coli is known to be a perfect direct electron transfer catalyst on electrodes of various origin. these features make the task of development of a high-yield reactivation protocol for rtop practically important. previous att ...	2015	25986322
site-directed mutagenesis of tobacco anionic peroxidase: effect of additional aromatic amino acids on stability and activity.	tobacco anionic peroxidase (top) is known to effectively catalyze luminol oxidation without enhancers, in contrast to horseradish peroxidase (hrp). to pursue structure-activity relationship studies for top, two amino acids have been chosen for mutation, namely thr151, close to the heme plane, and phe140 at the entrance to the active site pocket. three mutant forms top f140y, t151w and f140y/t151w have been expressed in escherichia coli, and reactivated to yield active enzymes. single-point mutat ...	2015	25957835
antipsychotics inhibit glucose transport: determination of olanzapine binding site in staphylococcus epidermidis glucose/h(+) symporter.	the antipsychotic drug olanzapine is widely prescribed to treat schizophrenia and other psychotic disorders. however, it often causes unwanted side effects, including diabetes, due to disruption of insulin-dependant glucose metabolism through a mechanism yet to be elucidated. to determine if olanzapine can affect the first step in glucose metabolism - glucose transport inside cells - we investigated the effect of this drug on the transport activity of a model glucose transporter. the glucose tra ...	2015	25941630
recombinant horseradish peroxidase: production and analytical applications.	horseradish peroxidase is a key enzyme in bio- and immunochemical analysis. new approaches in functional expression of the peroxidase gene in e. coli cells and the subsequent refolding of the resulting protein yield a recombinant enzyme that is comparable in its spectral and catalytic characteristics to the native plant peroxidase. genetic engineering approaches allow production of recombinant peroxidase conjugates with both protein antigens and fab antibody fragments. the present article review ...	2015	25869357
continuous colorimetric assay that enables high-throughput screening of n-acetylamino acid racemases.	n-acetyl amino acid racemases (naaars) have demonstrated their potential in the enzymatic synthesis of chiral amino acids, molecules of significant biotechnology interest. in order to identify novel activities and to improve these enzymes by engineering approaches, suitable screening methods are necessary. previous engineering of the naaar from amycolatopsis ts-1-60 was achieved by relying on an in vivo selection system that linked the viability of an e. coli l-methionine auxotroph to the activi ...	2015	25716802
magic: an automated n-linked glycoprotein identification tool using a y1-ion pattern matching algorithm and in silico ms² approach.	glycosylation is a highly complex modification influencing the functions and activities of proteins. interpretation of intact glycopeptide spectra is crucial but challenging. in this paper, we present a mass spectrometry-based automated glycopeptide identification platform (magic) to identify peptide sequences and glycan compositions directly from intact n-linked glycopeptide collision-induced-dissociation spectra. the identification of the y1 (peptidey0 + glcnac) ion is critical for the correct ...	2015	25629585
canine distemper virus infection among wildlife before and after the epidemic.	in 2007-2008, a canine distemper virus (cdv) epidemic occurred among wild animals in wakayama prefecture, japan, and many mammals, including the wild boar and deer, were infected. in this study, cdv prevalence among wild animals was surveyed before and after the epidemic. at first, an enzyme-linked immunosorbent assay (elisa) with horseradish peroxidase-conjugated protein a/g was established to detect cdv antibodies in many mammalian species. this established elisa was available for testing dogs ...	2015	26074342
the fucomic potential of mosquitoes: fucosylated n-glycan epitopes and their cognate fucosyltransferases.	fucoconjugates are key mediators of protein-glycan interactions in prokaryotes and eukaryotes. as examples, n-glycans modified with the non-mammalian core α1,3-linked fucose have been detected in various organisms ranging from plants to insects and are immunogenic in mammals. the rabbit polyclonal antibody raised against plant horseradish peroxidase (anti-hrp) is able to recognize the α1,3-linked fucose epitope and is also known to specifically stain neural tissues in the fruit fly drosophila me ...	2015	26617287
combining protein and strain engineering for the production of glyco-engineered horseradish peroxidase c1a in pichia pastoris.	horseradish peroxidase (hrp), conjugated to antibodies and lectins, is widely used in medical diagnostics. since recombinant production of the enzyme is difficult, hrp isolated from plant is used for these applications. production in the yeast pichia pastoris (p. pastoris), the most promising recombinant production platform to date, causes hyperglycosylation of hrp, which in turn complicates conjugation to antibodies and lectins. in this study we combined protein and strain engineering to obtain ...	2015	26404235
development and evaluation of an in-house igm-capture elisa for the detection of chikungunya and its application to a dengue outbreak situation in kenya in 2013.	chikungunya (chik) is a mosquito-borne viral disease. in the 2004 chik outbreak in kenya, diagnosis was delayed because of the lack of accurate diagnostics. therefore, this study aimed to develop and evaluate an in-house igm-capture enzyme linked immunosorbent assay (elisa) (in-house elisa) for the detection of chikungunya virus (chikv) infections. anti-chikv antibodies were raised in rabbits, purified and conjugated to horseradish peroxidase. these anti-chikv antibodies and cell-culture derived ...	2015	25866119
multifunctional fe₃o₄ core/ni-al layered double hydroxides shell nanospheres as labels for ultrasensitive electrochemical immunoassay of subgroup j of avian leukosis virus.	a novel electrochemical immunosensor for ultrasensitive detection of subgroup j of avian leukosis virus (alvs-j) was designed by using graphene sheets (gs)-layered double hydroxides (ldhs) composites modified electrode with multifunctional fe(3)o(4) core/ni-al ldhs shell (ldhs@fe(3)o(4)) nanospheres as labels. at first, the gs-ldhs were used for the immunosensor platform for improving the electronic transmission rate as well as increasing the surface area to capture a large amount of primary ant ...	2015	22621982
[immunochemical detection of azospirilla in soil with genus-specific antibodies].	immunoelectrophoresis and immunodiffusion analysis with antibodies to whole intact cells of the type strain of nitrogen-fixing soil bacteria azospirillum brasilense sp7 revealed at least three conservative surface immunogenic proteins of azospirilla. cross-reactions with these proteins made it possible to use the above antibodies for detection of azospirilla as a genus-specific probe conjugated with horseradish peroxidase as an enzymatic label. direct immune-enzyme analysis of soil suspensions ( ...	2015	26263631
monoclonal antibodies specific to human δ42pd1: a novel immunoregulator potentially involved in hiv-1 and tumor pathogenesis.	we recently reported the identification of δ42pd1, a novel alternatively spliced isoform of human pd1 that induces the production of pro-inflammatory cytokines from human peripheral blood mononuclear cells and enhances hiv-specific cd8(+) t cell immunity in mice when engineered in a fusion dna vaccine. the detailed functional study of δ42pd1, however, has been hampered due to the lack of a specific monoclonal antibody (mab). in this study, we generated 2 high-affinity mabs, clones ch34 (igg2b) a ...	2015	25692916
an enhanced sensitive electrochemical immunosensor based on efficient encapsulation of enzyme in silica matrix for the detection of human immunodeficiency virus p24.	we report a new electrochemical immunosensor for enhanced sensitive detection of human immunodeficiency virus p24 (hiv-p24) based on graphene oxide (go) as a nanocarrier and enzyme encapsulated in carbon nanotubes-silica as a matrix in a multienzyme amplification strategy. greatly enhanced sensitivity was achieved by using the bioconjugates featuring horseradish peroxidase-hiv-p24 signal antibody (hrp-hiv-p24) linked to functionalized go and thionine (th) as well as efficient encapsulation of en ...	2015	25240959
[development and assessment of a chemiluminescence assay kit for detecting anti-hev igg].	to develop an anti-hepatitis e virus (anti-hev) igg chemiluminescence assay kit and assess its clinical application.	2015	25940294
employment of 4-(1,2,4-triazol-1-yl)phenol as a signal enhancer of the chemiluminescent luminol-h2o2-horseradish peroxidase reaction for detection of hepatitis c virus in real samples.	highly sensitive detection of hepatitis c virus (hcv) in serum is a key method for diagnosing and classifying the extent of hcv infection. in this study, a p-phenol derivative, 4-(1,2,4-triazol-1-yl)phenol (4-trp), was employed as an efficient enhancer of the luminol-hydrogen peroxide (h2o2)-horseradish peroxidase (hrp) chemiluminescence (cl) system for detection of hcv. compared with a traditional enhancer, 4-trp strongly enhanced cl intensity with the effect of prolonging and stabilizing light ...	2015	25820800
electrochemical dna biosensor based on a tetrahedral nanostructure probe for the detection of avian influenza a (h7n9) virus.	a dna tetrahedral nanostructure-based electrochemical biosensor was developed to detect avian influenza a (h7n9) virus through recognizing a fragment of the hemagglutinin gene sequence. the dna tetrahedral probe was immobilized onto a gold electrode surface based on self-assembly between three thiolated nucleotide sequences and a longer nucleotide sequence containing complementary dna to hybridize with the target single-stranded (ss)dna. the captured target sequence was hybridized with a biotiny ...	2015	25844798
a general method for synthesizing enzyme-polymer conjugates in reverse emulsions using pluronic as a reactive surfactant.	using aldehyde-functionalized pluronic as the reactive surfactant, enzyme-pluronic conjugates with sizes ranging from nanometers to micrometers were synthesized in reverse emulsions. compared with the direct conjugation in aqueous solution, this method gave an increased conjugation efficiency and well-controlled size of the conjugates. the versatility of this method was validated using horseradish peroxidase (hrp), candida rugosa lipase (crl) and candida antarctica lipase b (calb). the resulting ...	2015	25977947
[establishment and evaluation of methods for determinating cystic fibrosis transmembrane conductance regulator quantitatively].	to establish and evaluate a ba-elisa method for the quantitative detection of cystic fibrosis transmembrane conductance regulator (cftr) protein.	2015	26248424
genomic overview of the phytopathogen pectobacterium wasabiae strain rns 08.42.1a suggests horizontal acquisition of quorum-sensing genes.	the blackleg and soft-rot diseases caused by pectinolytic enterobacteria such as pectobacterium and dickeya are major causes of losses affecting potato crop in the field and upon storage. in this work, we report the isolation, characterization and genome analysis of the pectobacterium wasabiae (formerly identified as pectobacterium carotovorum subsp. carotovorum) strain rns 08.42.1a, that has been isolated from a solanum tuberosum host plant in france. comparative genomics with 3 other p. wasabi ...	2015	25297844
enzymatically crosslinked dendritic polyglycerol nanogels for encapsulation of catalytically active proteins.	the enormous potential of nanogel scaffolds for protein encapsulation has been widely recognized. however, constructing stable polymeric nanoscale networks in a facile, mild, and controllable fashion still remains a technical challenge. here, we present a novel nanogel formation strategy using horseradish peroxidase (hrp) catalyzed crosslinking on phenolic derivatized dendritic polyglycerol (dpg) in the presence of h2o2 in an inverse miniemulsion. this "enzymatic nanogelation" approach was effic ...	2015	25519490
synthetic antibodies and peptides recognizing progressive multifocal leukoencephalopathy-specific point mutations in polyomavirus jc capsid viral protein 1.	polyomavirus jc (jcv) is the causative agent of progressive multifocal leukoencephalopathy (pml), a rare and frequently fatal brain disease that afflicts a small fraction of the immune-compromised population, including those affected by aids and transplantation recipients on immunosuppressive drug therapy. currently there is no specific therapy for pml. the major capsid viral protein 1 (vp1) involved in binding to sialic acid cell receptors is believed to be a key player in pathogenesis. pml-spe ...	2015	25879139
plasmonic enzyme-linked immunosorbent assay using nanospherical brushes as a catalase container for colorimetric detection of ultralow concentrations of listeria monocytogenes.	plasmonic enzyme-linked immunosorbent assay (pelisa) based on catalase (cat)-mediated gold nanoparticle growth exhibits ultrahigh sensitivity for detecting disease-related biomarkers using sandwich formats. however, the limit of detection (lod) of this strategy for listeria monocytogenes is only around 10(3) cfu/ml, which considerably exceeds the amount of l. monocytogenes commonly present in food products (<100 cfu/g). herein, we report an improved pelisa method for detection of l. monocytogene ...	2015	26646325
ultrasensitive detection and rapid identification of multiple foodborne pathogens with the naked eyes.	in this study, a novel approach for ultrasensitive detection and rapid high-throughput identification of a panel of common foodborne pathogens with the naked eyes is presented. as a proof-of-concept application, a multiple pathogen analysis array is fabricated through immobilizing three specific polyt-capture probes which can respectively recognize rfbe gene (escherichia coli o157:h7), inva gene (salmonella enterica), inla gene (listeria monocytogenes) on the plastic substrates. pcr has been dev ...	2015	25909338
rapid legionella pneumophila determination based on a disposable core-shell fe₃o₄@poly(dopamine) magnetic nanoparticles immunoplatform.	a novel amperometric magnetoimmunoassay, based on the use of core-shell magnetic nanoparticles and screen-printed carbon electrodes, was developed for the selective determination of legionella pneumophila sg1. a specific capture antibody (ab) was linked to the poly(dopamine)-modified magnetic nanoparticles (mnps@pda-ab) and incubated with bacteria. the captured bacteria were sandwiched using the antibody labeled with horseradish peroxidase (ab-hrp), and the resulting mnps@pda-ab-legionella neumo ...	2015	26320785
structure-based discovery of the first non-covalent inhibitors of leishmania major tryparedoxin peroxidase by high throughput docking.	leishmaniasis is a neglected vector-born disease caused by a protozoan of the genus leishmania and affecting more than 1.300.000 people worldwide. the couple tryparedoxin/tryparedoxin peroxidase is essential for parasite survival in the host since it neutralizes the hydrogen peroxide produced by macrophages during the infection. herein we report a study aimed at discovering the first class of compounds able to non-covalently inhibit tryparedoxin peroxidase. we have solved the high-resolution str ...	2015	25951439
effects of different media supplements on the production of an active recombinant plant peroxidase in a pichia pastoris δoch1 strain.	recombinant protein production in microorganisms is one of the most studied areas of research in biotechnology today. in this respect the yeast pichia pastoris is an important microbial production host due to its capability of secreting the target protein and performing posttranslational modifications. in a recent study, we described the development of a robust bioprocess for a glyco-engineered recombinant p. pastoris strain where the native α-1,6-mannosyltransfrease och1 was knocked out (δoch1 ...	2015	25837321
optimizing cofactor availability for the production of recombinant heme peroxidase in pichia pastoris.	insufficient incorporation of heme is considered a central impeding cause in the recombinant production of active heme proteins. currently, two approaches are commonly taken to overcome this bottleneck; metabolic engineering of the heme biosynthesis pathway in the host organism to enhance intracellular heme production, and supplementation of the growth medium with the desired cofactor or precursors thereof to allow saturation of recombinantly produced apo-forms of the target protein. in this stu ...	2015	25586641
induction of oxidative stress in prototheca zopfii by indole-3-acetic acid/hrp or 2,4-pentanedione/hrp systems and their oxidation products.	we investigated the toxic effects on prototheca zopfii of indole-3-acetic acid (iaa) and 2,4-pentanedione (pd) combined with horseradish peroxidase (hrp) alongside the oxidation products of 3-methyl-2-oxindole (moi) and indole-3-carbinol (i3c) from the iaa/hrp system and methylglyoxal (mgo) from the pd/hrp system. the microorganism was incubated in the absence (control) or presence of iaa, pd, iaa/hrp, pd/hrp, moi, i3c and mgo and determined: (1) cytotoxicity by mtt (3-(4,5-dimethylthiazol-2-yl) ...	2015	25173924
[a new mannose-specific lectin from daylily (hemerocallis fulva l.) rhizome: purification and properties].	a new lectin was purified from the daylily (hemerocallis fulva l.) with the yield of approximately 10 mg per kg of fresh plant rhizome. the purification procedure was based on application of the affinity chromathography on the column with yeast mannan and the ion-exchange chromatography on the column with deae-toyopearl. the lectin possessed low affinity for alpha-methyl-d-mannopyranoside, d-fructose, d-turanose and 2-acetamido-d-galactopyranose and hight affinity for the yeast mannan. the lecti ...	2015	23808307
aptamer-phage reporters for ultrasensitive lateral flow assays.	we introduce the modification of bacteriophage particles with aptamers for use as bioanalytical reporters, and demonstrate the use of these particles in ultrasensitive lateral flow assays. m13 phage displaying an in vivo biotinylatable peptide (avitag) genetically fused to the phage tail protein piii were used as reporter particle scaffolds, with biotinylated aptamers attached via avidin-biotin linkages, and horseradish peroxidase (hrp) reporter enzymes covalently attached to the pviii coat prot ...	2015	26456715
estimation of free radical-quenching activity of leaf pigment extracts.	the chromogen 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) radical (abts.+) can be directly generated by the enzymatic system formed by hydrogen peroxide and horseradish peroxidase in ethanolic media. the reaction is time- and concentration-dependent, and the abts.+ generated shows excellent stability. the method is an adaptation of the decolouration method previously reported (cano et al. 1998. phytochem anal 9:196-202), which was used for estimating anti-oxidant activity in aqueous ...	2015	11705243
raman evidence that the lyoprotectant poly(ethylene glycol) does not restore nativity to the heme active site of horseradish peroxidase suspended in organic solvents.	resonance raman spectroscopy was used to interrogate the heme active site of horseradish peroxidase (hrp) lyophilized in the presence and absence of the lyoprotectant poly(ethylene glycol) (peg; fw 5000; 0-80% w/w) suspended in acetone, chloroform, or acetonitrile. in aqueous solution, fe(3+)hrp is characterized by a five-coordinate high-spin (5-c hs) heme system. the structure of the heme-active site of hrp in all solvents is perturbed by co-lyophilization of hrp with peg. heme active site stru ...	2015	12099832
use of additives to enhance the removal of phenols from water treated with horseradish and hydrogen peroxide.	use of additives, such as polyethylene glycol (peg), selected surfactants, chitosan gel, or activated carbon, has been shown to enhance enzymatic treatment of water polluted with organic compounds. in this study, additives were used to facilitate the removal of 2,4-dichlorophenol (2,4-dcp) from water using minced horseradish (armoracia rusticana p. gaertn. et al.) as a carrier of peroxidase activity. the specific objectives of the study were to (i) enhance the pollutant removal activity of mince ...	2015	12931875
synthesis and gelation of dopa-modified poly(ethylene glycol) hydrogels.	3,4-dihydroxyphenylalanine (dopa) residues are known for their ability to impart adhesive and curing properties to mussel adhesive proteins. in this paper, we report the preparation of linear and branched dopa-modified poly(ethylene glycol)s (peg-dopas) containing one to four dopa endgroups. gel permeation chromatography-multiple-angle laser light scattering analysis of methoxy-peg-dopa in the presence of oxidizing reagents (sodium periodate, horseradish peroxidase, and mushroom tyrosinase) reve ...	2015	12217051
[a sensitive method for quantitative analysis of hydrogen peroxide and oxidase substrates in biological samples].	the sensitive method for peroxidative assays of hydrogen peroxide (up to 5 nmole in 1 ml) and oxidases' substrates (glucose, ethanol) has been developed by use of a non-cancerogenic chromogen--3,3', 5,5'-tetramethylbenzidine (tmb), horseradish peroxidase and corresponding oxidases. the proposed modification of the known method consists in using decreased concentration of tmb (0.05-0.1 mm) and stopping the reaction by adding of acid to ph 1.4-2.1. this approach allowed to enhance the sensitivity ...	2015	10445279
enzymatic microreactors in chemical analysis and kinetic studies.	the fields of application of microreactors are becoming wider every year. a considerable number of papers have been published recently reporting successful application of enzymatic microreactors in chemistry and biochemistry. most are devices with enzymes immobilized on beads or walls of microfluidic channels, whilst some use dissolved enzymes to run a reaction in the microfluidic system. apart from model systems, mostly with glucose oxidase, horseradish peroxidase and alkaline phosphatase, the ...	2015	16055295
[effect of temperature on structure and functional properties of horseradish peroxidase].	the dynamics of structural and functional changes proceeding in a peroxidase molecule under the effect of temperature was studied. it was shown that peroxidase thermoinactivation proceeds in two consequent stages. based on the analysis of enzyme peroxidase and oxidase activity and peroxidase spectral and buffer characteristics, it was established that at temperatures from 20 to 55 degrees c reversible conformation there occur changes of the hemoprotein molecule related to consequent unfolding an ...	2015	14577150
cortical target depletion and the developing lateral geniculate nucleus: implications for trophic dependence.	the dependence of the developing dorsal lateral geniculate nucleus (lgd) on visual cortex for survival has been well documented. complete removal of visual cortex during early postnatal development results in degeneration of the lgd. to further explore the nature of this trophic relationship, we depleted variable proportions of the principal targets of geniculocortical axons, layer iv neurons, and also variable proportions of the supragranular neurons by intraperitoneal injections of different d ...	2015	8670670
effect of polymers on enhanced chemiluminescent assays for peroxidase and peroxidase labels.	hydroxypropyl methylcellulose, hydroxyethyl cellulose, and hydroxybutyl methylcellulose stabilized light emission in a boronic acid-enhanced chemiluminescent assay for horseradish peroxidase. the stabilization of light emission was concentration-dependent and more effective with substituted boronic acid enhancers (e.g. 4-iodophenylboronic acid) than with substituted phenol enhancers (e.g. 4-iodophenol). hydroxybutyl methylcellulose improved the linearity of the dose-response curve in a peroxidas ...	2015	9343314
enhancer effect of fluorescein on the luminol-h2o2-horseradish peroxidase chemiluminescence: energy transfer process.	the chemiluminescence of the luminol-h2o2-horseradish peroxidase system is increased by fluorescein. fluorescein produces an enhancement of the luminol chemiluminescence similar to that of phenolphthalein, by an energy transfer process from luminol to fluorescein. the maximum intensity and the total chemiluminescence emission (between 380 and 580 nm) of luminol with fluorescein was more than three times greater than without fluorescein; however, the emission duration was shorter. the emission sp ...	2015	9481607
[the use of monoclonal antibodies for the quantitative determination of myoglobin by the solid phase immunoenzyme "sandwich" assay].	a procedure of solid phase single step immunoenzyme assay of the "sandwich" type involving monoclonal antibodies was developed to estimate myoglobin in blood serum of patients, 30 stable hybridomic clones. producing antibodies to not less than six various epitopes of myoglobin, were obtained. content of myoglobin in blood serum was studied using antibodies of the igg type to two various epitopes of myoglobin as well as of their conjugates with horseradish peroxidase. the procedure developed enab ...	2015	3057738
[a spectrophotometric method for determining the concentration of l-lysine using l-lysine-alpha-oxidase from trichoderma sp. and 3,3',5,5'-tetramethylbenzidene dihydrochloride].	a simple and relatively sensitive procedure was developed for determination of l-lysine at 3-30 mmole/l concentration. the procedure does not involve the carcinogenic compound o-dianisidine. l-lysine alpha-oxidase catalyzed oxidative deamination of l-lysine with o2 consumption and formation of h2o2, nh3 and alpha-keto-epsilon-aminocaproic acid. horseradish peroxidase and a non-carcinogenic compound 3,3,5,5'-tetramethylbenzidine dihydrochloride as an electron donor were used in determination of h ...	2015	1949695
[a neuroanatomical study of the vestibular efferent system, iii: the origin and pathway of the axons].	we have studied the vestibular efferent fibers pathway, after scratching the peripheral vestibular receptors and horseradish peroxidase injection, and tmb or dab processing. we have proved the existence of a differentiated bundle in the main vestibular pathway, this bundle crosses the areas where the efferent somas have been situated, some of this fibers have been seen crossing the midline. it has not been possible to connect the efferent somas with the fibers.	2015	1907835
[physical separation of the populations of cochlear and vestibular efferent somas. a study using horseradish peroxidase].	we have physically separated cochlear and vestibular end organs, and after injecting horseradish peroxidase two different populations of efferent somas have been described belonging to the cochlea and vestibule.	2015	1742064
ultrastructure and permeability of immature neural and extraneural blood vessels.	the ultrastructure and permeability to the marker horseradish peroxidase in the leptomeningeal and neural vessels of the optic lobes, and in the wing bud vessels, were compared, during early stages of chick embryo vasculogenesis, in order to ascertain whether young neural vessels, like mature ones, possess structural specificity preventing the free transport of molecules through their wall (blood-brain barrier). the results demonstrated that immature endothelia are always permeable to the tracer ...	2015	2091805
[isolation of enzymes from mixed reversed micelles of surface-active agents].	the catalytic activities of lysozyme, horseradish peroxidase (hp), catalase, glucose-6-phosphate dehydrogenase (g6pdh) and lactate dehydrogenase (ldh) were studied in aqueous solutions and after isolation of the enzymes from mixed reversed micelles of aerosol ot and triton x-45 by organic solvents (acetone, ethanol, isopropanol), by acetone-water mixtures, as well as by aqueous solutions containing urea, glycerol, polyethylene glycol 6000 and ammonium sulphate. the isolation conditions were foun ...	2015	2453051
[efferent neurons of the auditory center in the midbrain of the frog rana ridibunda].	individual cells which produce projections from the torus semicircularis in the frog have been visualized after injection of horseradish peroxidase (hrp) to various thalamic and isthmal areas. labeled toral cells were observed if hrp had been injected to the posterodorsal areas of the thalamus or to the isthmal areas where lateral lemniscus fibers and cells of the premature lateral lemniscal nucleus are situated. medium and large size cells in the rostrolateral torus semicircularis were mainly l ...	2015	2431564
vascular permeability in transplantable murine gliomas: morphological correlation with tracer studies.	invasive astrocytomas were produced in mice by intracerebral injection of a cell line obtained from a spontaneous murine astrocytoma. these tumours grew in the cerebral hemispheres and, in many cases, extended through the needle hole in the skull to give rise to large extracranial tumours. on injection of the tracers, evans' blue or horseradish peroxidase (hrp), into the femoral vein, differences were noted in the vascular permeability of the intracerebral and extracranial tumours; the latter al ...	2015	2443863
differential retrograde labelling with horseradish peroxidase (hrp) and lucifer yellow (ly) in an invertebrate nervous system--hrp fluorescence and ly preservation limit choice of fixative.	the possible application of the combination of horseradish peroxidase (hrp) and lucifer yellow ch (ly) for differential retrograde labelling of a relatively large invertebrate nervous system requiring the enzymatic marker to be processed after fixation and sectioning was evaluated. in control experiments an hrp fluorescence induced by glutaraldehyde (ga) fixation was detected and its spectral properties were investigated. this fluorescence, however, does not occur after previous treatment of the ...	2015	4079461
how do the basal ganglia and cerebellum gain access to the cortical motor areas?	we have used retrograde transport of wheat germ agglutinin conjugated to horseradish peroxidase to examine the origin of the thalamic input to the two premotor areas with the densest projections to the motor cortex. these are: arcuate premotor area (apa) and the supplementary motor area (sma). retrograde transport demonstrated that the two premotor areas and the motor cortex each receive thalamic input from separate, cytoarchitectonically well-defined subdivisions of the ventrolateral thalamus. ...	2015	3913443
neuroanatomical evidence for electroreception in lampreys.	the patterns of the anterior lateral-line afferents of lampetra fluviatilis as revealed by transganglionic transport of horseradish peroxidase are described. the afferents form two roots in entering the rhombencephalon. fibers of the dorsalmost root can be traced to a short dorsal fascicle which runs along the dorsal nucleus. the ventral roots form two fascicles adjacent to the nucleus intermedius. comparison with urodeles indicates that lampreys, like urodeles and other anamniotic vertebrates a ...	2016	6208702
production of a conjugate between the rk346 antigen from leishmania infantum and the horseradish peroxidase c for the detection of rk346 antibodies.	it was designed and characterized a reporter system to be captured by an- tibodies bound to elisa plates. the system was designed with the rk346 from leishmania infantum, a highly antigenic and specific protein. the rk346 was coupled to the horseradish peroxidase c (hrpc) from armoracia rusticana using glutaraldehyde or sulfo-smcc. gluta- raldehyde conjugation was performed in two steps. separation of conjugates was carried out using a sepharose s-200 in size exclusion chromatography (sec); frac ...	2016	28429896
arterial air embolism: structural effects on the gerbil brain.	air injection into the carotid artery of adult mongolian gerbils caused, within 10 minutes, multifocal brain lesions. the extracellular spaces were widened and neurons, oligodendrocytes and myelin sheaths remained unchanged. the "delayed" effects of air embolism (first seen after 3 h) were similar to those observed in gerbils after unilateral carotid ligation. the histologic alterations after 3 h consisted of astrocytic swelling and shrinkage/necrosis of neuronal soma. the observations reported ...	2016	7314163
effect of hypertension on blood-brain barrier. change after restoration of blood flow in post-ischemic gerbil brains. an electronmicroscopic study.	the effect of induced hypertension on the blood-brain barrier (bbb) change in mongolian gerbils exposed to various periods of ischemia was studied. evans blue dye was used to determine the bbb change in animals subjected to different levels of hypertension after 3 h ischemia. horseradish peroxidase (hrp) was used in electronmicroscopic studies of animals subjected to 30 min, 1, 3 or 6 h ischemia and subsequently exposed for 30 min to varying periods and sequences of normo- and hypertension. furt ...	2016	7210066
evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress.	the use of dichlorofluorescin (dcfh) as a measure of reactive oxygen species was studied in aqueous media. hydrogen peroxide oxidized dcfh to fluorescent dichlorofluorescein (dcf), and the oxidation was amplified by the addition of ferrous iron. hydrogen peroxide-induced dcf formation in the presence of ferrous iron was completely inhibited by deferoxamine and partially inhibited by ethylenediaminetetraacetic acid, but was augmented by diethylenetriaminepentaacetic acid. iron-peroxide-induced ox ...	2016	1322737
block copolymer microdomains: a novel medium for enzymatic reactions.	block copolymers exhibit the phenomenon of microdomain formation in pure states as well as in solutions. the microdomains vest the block copolymer assemblies with the intriguing characteristics of microheterogeneous media. we demonstrate that this microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. examples involving cholesterol oxidase and horseradish peroxidase are provided here. the observed chan ...	2016	1367345
new enhancers for the chemiluminescent peroxidase catalysed chemiluminescent oxidation of pyrogallol and purpurogallin.	the effects of various boronate compounds, 4-biphenylboronic acid, 4-bromobenzene-boronic acid, trans-4-(3-propionic acid)phenylboronic acid and 4-iodophenylboronic acid, on the horseradish peroxidase (hrp) catalysed chemiluminescent oxidation of pyrogallol and purpurogallin by peroxide were investigated. trans-4-(3-propionic acid)phenylboronic acid produced a 13.7-fold enhancement in the peak light emission from the chemiluminescent hrp catalysed pyrogallol reaction (detection limit for hrp < 1 ...	2016	7676857
simultaneous screening of fumonisin b1, aflatoxin b1, and zearalenone by line immunoblot: a computer-assisted multianalyte assay system.	a line immunoblot assay was developed for the simultaneous screening of fumonisin b1 (fb1), aflatoxin b1 (afb1), and zearalenone (zea). monoclonal antibodies for each of these toxins were immobilized as multiple lines on nitrocellulose membrane strips and sectored into hydrophobic compartments to minimize use of reagents. a modified enzyme-linked immunosorbent assay was conducted whereby free mycotoxins and horseradish peroxidase-labeled mycotoxins competed for binding to the nitrocellulose-boun ...	2016	8199485
selective formation of oxindole- and formylkynurenine-type products from tryptophan and its peptides treated with a superoxide-generating system in the presence of iron(iii)-edta: a possible involvement with iron-oxygen complex.	the oxygenation of tryptophan and its peptides by the superoxide-generating system hypoxanthine/xanthine oxidase in the presence of iron(iii) and ethylenediaminetetraacetic acid (edta) has been investigated. the reaction of a tryptophan derivative, n-(tert-butoxycarbonyl)-l-tryptophan, with hypoxanthine/xanthine oxidase/fe(iii)-edta mainly resulted in the oxygenation of the pyrrole ring of the indole nucleus. 2-[(tert-butoxycarbonyl)-amino]-3-(3-oxindolyl)propionic acid and n-(tert-butoxycarbony ...	2016	8199307
a 56- to 54-kilodalton non grata signal in immunoblot analysis using the horseradish peroxidase chemiluminescence system.	the major advantages of the horseradish peroxidase chemiluminescence (hrp-cl) immunodetection method in western blot analysis are its high sensitivity, nonradioactive detection, economy of the primary antibody, and speed of detecting the signal. however, we observed a strong and reproducible signal that was detected regardless of the primary antibody and of the cell type used. this signal, present at 56-54 kilodaltons (kda), is generated in absence of any primary antibody and seems to be an intr ...	2016	7840944
quantitative estimation of hrp-labeled sensory and motor neurons during nerve-dependent and nerve-independent periods of urodele limb regeneration.	the relationship between urodele regeneration and possible regeneration in mammalian prospects is hard to evidence, but the idea of possible regeneration of neural elements in people is an area of potential clinical importance that is under investigation. one of the great challenges of the future is to understand enough about the basic biology of animal regeneration and to use it for the betterment of the mankind. it is well established that the initial stages of urodele limb regeneration depend ...	2016	12942746
enzymatic copolymerization alters the structure of unpolymerized mixtures of the biomimetic monomers: the amphiphilic decyl ester of l-tyrosine and l-tyrosineamide--an afm investigation of nano- to micrometer-scale structure differences.	previously, we have shown that the amphiphilic decyl esters of both d- and l-tyrosine (delt) self-assemble in aqueous solution above their critical micelle concentration values to form long rodlike structures that can be enzymatically polymerized. in the current study, we have examined the self-assembled structures of unpolymerized and enzymatically (horseradish peroxidase) copolymerized 1:1 molar mixtures of delt with the nonamphiphilic comonomer l-tyrosineamide. the structures were examined fo ...	2016	15360300
a nitric oxide biosensor based on horseradish peroxidase/kieselguhr co-modified pyrolytic graphite electrode.	a reagentless nitric oxide (no) biosensor was prepared using a pyrolytic graphite (pg) electrode modified with a composite film containing horseradish peroxidase (hrp) and kieselguhr. noticeably, the electron-transfer reactivity of hrp was significantly enhanced when incorporated in the inorganic kieselguhr material. consequently, we observed the direct electrochemical response of hrp in this composite film, which would be otherwise electrochemically silent (in the absence of kieselguhr). import ...	2016	15279105
specific and selective electrochemical immunoassay for pseudomonas aeruginosa based on pectin-gold nano composite.	in this report, we have successfully fabricated an immunosensor for detection of pseudomonas aeruginosa in water. the monoclonal antibody was immobilized on the surface modified with cclp (calcium cross-linked pectin)-au nps (gold nanoparticles)/glassy carbon electrode. the building of the immunosensor was evaluated in each step by cyclic voltammetry (cv) and impedance spectroscopy (eis). the electrochemical detection was done based on the anti rabbit igg hrp (horseradish peroxidase) which binds ...	2016	26703990
biotechnological advances towards an enhanced peroxidase production in pichia pastoris.	horseradish peroxidase (hrp) is a high-demand enzyme for applications in diagnostics, bioremediation, biocatalysis and medicine. current hrp preparations are isolated from horseradish roots as mixtures of biochemically diverse isoenzymes. thus, there is a strong need for a recombinant production process enabling a steady supply with enzyme preparations of consistent high quality. however, most current recombinant production systems are limited at titers in the low mg/l range. in this study, we u ...	2016	27432633
[inhibitory effect of cabozantinib against listeria monocytogenes invasion in caco-2 cells in vitro].	to investigate the effect of c-met inhibitor cabozantinib (xl-184) in inhibiting listeria monocytogenes (lm) from invading caco-2 cells to reduce the cell injury.	2016	26922021
highly active, stable and self-antimicrobial enzyme catalysts prepared by biomimetic mineralization of copper hydroxysulfate.	a nature-inspired approach to encapsulate enzymes in spindle-like copper hydroxysulfate nanocrystals was developed by a biomimetic mineralization process. several types of enzymes including glucose oxidase (gox), horseradish peroxidase (hrp), candida antarctica lipase b (calb) and cytochrome c (cyt c) were successfully encapsulated in copper hydroxysulfate nanocrystals quickly (within 1 hour) with maintained or even greatly enhanced catalytic efficiencies (kcat/km of cyt c showed a 143-fold incr ...	2016	27714119
transfer of the potato plant isolates of pectobacterium wasabiae to pectobacterium parmentieri sp. nov.	pectobacterium wasabiae was originally isolated from japanese horseradish (eutrema wasabi), but recently some pectobacterium isolates collected from potato plants and tubers displaying blackleg and soft rot symptoms were also assigned to p. wasabiae. here, combining genomic and phenotypical data, we re-evaluated their taxonomic position. pacbio and illumina technologies were used to complete the genome sequences of p. wasabiae cfbp 3304t and rns 08-42-1a. multi-locus sequence analysis showed tha ...	2016	27692046
a recombinant positive control for serology diagnostic tests supporting elimination of onchocerca volvulus.	serological assays for human igg4 to the onchocerca volvulus antigen ov16 have been used to confirm elimination of onchocerciasis in much of the americas and parts of africa. a standardized source of positive control antibody (human anti-ov16 igg4) will ensure the quality of surveillance data using these tests.	2016	26745374
a comparison between the oxidation with laccase and horseradish peroxidase for triclosan conversion.	triclosan is a broad-spectrum biocide used in personal-care products that is suspected to be linked to the emergence of antibiotic-resistant bacteria. in the present work, the enzymes horseradish peroxidase and laccase from trametes versicolor were evaluated for the conversion of triclosan in an aqueous matrix. the removal of antibacterial activity by the enzymatic processes was evaluated by an assay based on the growth inhibition of escherichia coli k12. the horseradish peroxidase (hrp) process ...	2016	26165135
specific determination of influenza h7n2 virus based on biotinylated single-domain antibody from a phage-displayed library.	the unpredicted spread of avian influenza virus subtype h7n2 in the world is threatening animals and humans. specific and effective diagnosis and supervision are required to control the influenza. however, the existing detecting methods are laborious, are time-consuming, and require appropriate laboratory facilities. to tackle this problem, we isolated vhh antibodies against the h7n2 avian influenza virus (aiv) and performed an enzyme-linked immunosorbent assay (elisa) to detect the h7n2 virus. ...	2016	26450565
a novel blocking elisa for detection of antibodies against hepatitis e virus in domestic pigs.	hepatitis e virus (hev) infects both humans and animals, with an overall human mortality rate generally less than 1%, but as high as 20% among pregnant women. hev strains fall into 4 major genotypes. zoonotic genotypes 3 and 4 associate with sporadic human and animal hev cases in many industrialized countries. to date, collective evidence implicates pigs as the main hev reservoir, justifying the importance of monitoring hev infection rates in pig herds to prevent human illness. due to the lack o ...	2016	27023902
functional specialty of cd40 and dendritic cell surface lectins for exogenous antigen presentation to cd8(+) and cd4(+) t cells.	dendritic cells (dcs) are major antigen-presenting cells that can efficiently prime and cross-prime antigen-specific t cells. delivering antigen to dcs via surface receptors is thus an appealing strategy to evoke cellular immunity. nonetheless, which dc surface receptor to target to yield the optimal cd8(+) and cd4(+) t cell responses remains elusive. herein, we report the superiority of cd40 over 9 different lectins and scavenger receptors at evoking antigen-specific cd8(+) t cell responses. ho ...	2016	27077111
sensitive impedimetric immunoassay of japanese encephalitis virus based on enzyme biocatalyzed precipitation on a gold nanoparticle-modified screen-printed carbon electrode.	a sensitive and disposable electrochemical impedance biosensor to detect japanese encephalitis virus (jev) was developed based on a gold nanoparticle (aunp)-modified screen-printed carbon electrode (spce). a biosensor was fabricated through covalent grafting of a mixed self-assembled monolayer on aunps with a specific antibody. to detect jev and achieve signal amplification, the horseradish peroxidase (hrp)-labeled second antibody was linked to the biosensor through a sandwich immunity reaction. ...	2016	27725612
rapid detection of bacillus anthracis spores using immunomagnetic separation and amperometry.	portable detection and quantitation methods for bacillus anthracis (anthrax) spores in pure culture or in environmental samples are lacking. here, an amperometric immunoassay has been developed utilizing immunomagnetic separation to capture the spores and remove potential interferents from test samples followed by amperometric measurement on a field-portable instrument. antibody-conjugated magnetic beads and antibody-conjugated glucose oxidase were used in a sandwich format for the capture and d ...	2016	27999382
glutathione protects candida albicans against horseradish volatile oil.	horseradish essential oil (hreo; a natural mixture of different isothiocyanates) had strong fungicide effect against candida albicans both in volatile and liquid phase. in liquid phase this antifungal effect was more significant than those of its main components allyl, and 2-phenylethyl isothiocyanate. hreo, at sublethal concentration, induced oxidative stress which was characterized with elevated superoxide content and up-regulated specific glutathione reductase, glutathione peroxidase, catalas ...	2016	27272511
genome sequence of pectobacterium carotovorum phage ppws1, isolated from japanese horseradish [eutrema japonicum (miq.) koidz] showing soft-rot symptoms.	italic! pectobacterium carotovorumsubsp. italic! carotovorumand its lytic bacteriophage ppws1 were isolated from a japanese horseradish rhizome with soft rot. sequencing of the phage genomic dna suggested that ppws1 is a new species of the family italic! podoviridaeand has high similarity to the bacteriophage peat1 infectious to italic! p. atrosepticum.	2016	27103734
construction of a horseradish peroxidase resistant toward hydrogen peroxide by saturation mutagenesis.	horseradish peroxidase (hrp) with a variety of potential biotechnological applications is still isolated from the horseradish root as a mixture of different isoenzymes with different biochemical properties. there is an increasing demand for preparations of high amounts of pure enzyme but its recombinant production is limited because of the lack of glycosylation in escherichia coli and different glycosylation patterns in yeasts which affects its stability parameters. the goal of this study was to ...	2016	26331237
proteinase k activity determination with β-galactosidase as sensitive macromolecular substrate.	proteinase k from engyodontium album (prok) is a relatively unspecific serine endopeptidase which is known to attack proteins yet in their native states. if the attacked protein is an enzyme, even a partial hydrolysis by prok may lead to an inactivation of the enzyme, which can be monitored by measuring the loss of catalytic activity of the attacked enzyme. e. coli β-galactosidase (β-gal) was used in this work as such enzyme. it was found to be a convenient and sensitive macromolecular model sub ...	2016	27594349
miniaturized flow stacked immunoassay for detecting escherichia coli in a single step.	commercially available systems that provide cost-effective, fast, simple, and portable solutions for health and environmental applications are few despite advancements in bioassays and biosensor research. we have developed a new system based on stacked membranes, each layer with a specific function. samples were added onto the bottom-most layer, and as each layer becomes wet, the analyte pushes through to the next membrane layers. during migration, the analyte attaches with the corresponding ant ...	2016	27218705
generation of e. coli-derived virus-like particles of porcine circovirus type 2 and their use in an indirect igg enzyme-linked immunosorbent assay.	porcine circovirus type 2 (pcv2) causes increased mortality and poor growth or weight loss in apparently healthy swine. therefore, methods to detect pcv2-specific antibodies in swine serum are important for prevention, diagnosis, and control of pcv2-associated diseases (pcvad). in this study, pcv2 virus-like particles (vlps) were used to develop a rapid, simple and economical indirect enzyme-linked immunosorbent assay to detect (with high sensitivity) pcv2-specific antibodies in swine serum. the ...	2016	26973229
optimized dendrimer-encapsulated gold nanoparticles and enhanced carbon nanotube nanoprobes for amplified electrochemical immunoassay of e. coli in dairy product based on enzymatically induced deposition of polyaniline.	a highly sensitive immunosensor was reported for escherichia coli assay in dairy product based on electrochemical measurement of polyaniline (pan) that was catalytically deposited by horseradish peroxidase (hrp) labels. herein, the immunosensor was developed by using poly(amidoamine) dendrimer-encapsulated gold nanoparticles (pamam(au)) as sensing platform. importantly, the optimal haucl4/pamam ratio was investigated to design the efficient pamam(au) nanocomposites. the nanocomposites were prove ...	2016	26908184
co-immobilization of multiple enzymes by metal coordinated nucleotide hydrogel nanofibers: improved stability and an enzyme cascade for glucose detection.	preserving enzyme activity and promoting synergistic activity via co-localization of multiple enzymes are key topics in bionanotechnology, materials science, and analytical chemistry. this study reports a facile method for co-immobilizing multiple enzymes in metal coordinated hydrogel nanofibers. specifically, four types of protein enzymes, including glucose oxidase, candida rugosa lipase, α-amylase, and horseradish peroxidase, were respectively encapsulated in a gel nanofiber made of zn(2+) and ...	2016	26932320
an experimental platform using human intestinal epithelial cell lines to differentiate between hazardous and non-hazardous proteins.	human intestinal epithelial cell lines (t84, caco-2, and hct-8) grown on permeable transwell™ filters serve as models of the gastrointestinal barrier. in this study, this in vitro model system was evaluated for effectiveness at distinguishing between hazardous and non-hazardous proteins. indicators of cytotoxicity (ldh release, mtt conversion), monolayer barrier integrity ([(3)h]-inulin flux, horseradish peroxidase flux, trans-epithelial electrical resistance [teer]), and inflammation (il-8, il- ...	2016	27060235
colorimetric integrated pcr protocol for rapid detection of vibrio parahaemolyticus.	rapid detection of pathogens is of great significance for food safety and disease diagnosis. a new colorimetric method for rapid and easy detection of vibrio parahaemolyticus (v. parahaemolyticus or vp) has been developed in this research. a specific sequence was designed and integrated with the forward primer for molecular detection of vp. this specific sequence was tested and treated as the horseradish peroxidase (hrp)-mimicking dnazyme and could be amplified during the polymerase chain reacti ...	2016	27690041