Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| biofouling characteristics and identification of preponderant bacteria at different nutrient levels in batch tests of a recirculating cooling water system. | understanding the influence of nutrient levels on biofouling control is an important requirement for management strategies in a recirculating cooling water system. nutrient limitation may be one way to control biofouling development without increasing biocide dosing. therefore, this study was carried out to investigate the effects of nutrient levels on biofouling characteristics and to identify the preponderant bacteria in the batch tests with a simulated cooling water system. the biofouling cha ... | 2011 | 21879565 |
| cutaneous gonococcal abscess of the abdomen in a child. | virtually all pediatric cases of neisseria gonorrhoeae originate from contact with an infected adult. a cutaneous abscess caused by n. gonorrhoeae in a child is extremely rare, especially outside the genital area. we report a case of a 22-month-old boy with a gonococcal cutaneous abscess on the abdominal wall and suggest that n. gonorrhoeae should be included in the differential diagnosis of skin and soft tissue infections in children. | 2011 | 21926887 |
| a snapshot of a pathogenic bacterium mid-evolution: neisseria meningitidis is becoming a nitric oxide-tolerant aerobe. | members of the neisseria genus typically display the ability to carry out denitrification of nitrite to nitrous oxide as an alternative to oxygen respiration when oxygen is depleted. the key enzymes nitrite and nitric oxide reductase are found across the neisseria genus. within neisseria meningitidis, however, a number of research groups have found that a significant proportion of strains lack a functional nitrite reductase. it appears that n. meningitidis is on an evolutionary trajectory toward ... | 2011 | 22103546 |
| neisseria base: a comparative genomics database for neisseria meningitidis. | neisseria meningitidis is an important pathogen, causing life-threatening diseases including meningitis, septicemia and in some cases pneumonia. genomic studies hold great promise for n. meningitidis research, but substantial database resources are needed to deal with the wealth of information that comes with completely sequenced and annotated genomes. to address this need, we developed neisseria base (nbase), a comparative genomics database and genome browser that houses and displays publicly a ... | 2011 | 21930505 |
| vaccine-preventable diseases and their impact on latin american children. | a joint meeting of the latin american society of pediatric infectious diseases, the dominican society of pediatrics and the dominican society of vaccinology was held in the dominican republic. this report highlights the most relevant issues that were presented and discussed about vaccine-preventable diseases, their epidemiology and impact in latin american children, the need to move forward and expand national immunization programs and the economical and political obstacles to introduce ?new? va ... | 2011 | 22085169 |
| genotyping of two neisseria gonorrhoeae fluroquinolone-resistant strains in the brazilian amazon region. | we report two ciprofloxacin and ofloxacin-resistant neisseria gonorrhoeae strains that were isolated from the urethral discharge of male patients at the sexually transmitted diseases outpatient clinic of the alfredo da matta foundation (manaus, state of amazonas, brazil). the gonococci displayed minimal inhibitory concentrations (> 32.00 µg/ml) and three mutations in the quinolone resistance-determining region (s91f and d95g in gyra and s87r in parc). both isolates were genotyped using n. gonorr ... | 2011 | 21894387 |
| molecular analyses of tem genes and their corresponding penicillinase-producing neisseria gonorrhoeae isolates in bangkok, thailand. | neisseria gonorrhoeae is a major public health problem globally, especially because the bacterium has developed resistance to most antimicrobials introduced for first-line treatment of gonorrhea. in the present study, 96 n. gonorrhoeae isolates with high-level resistance to penicillin from 121 clinical isolates in thailand were examined to investigate changes related to their plasmid-mediated penicillin resistance and their molecular epidemiological relationships. a β-lactamase (tem) gene varian ... | 2011 | 22143532 |
| lower genital tract infections in hiv-seropositive women in india. | the presence of std facilitates shedding of hiv and increases hiv-1 disease progression, possibly by increasing plasma viremia. our aim was to study the presence of various associated sexually transmitted disease/reproductory tract infections in hiv-seropositive women in india. | 2011 | 22021972 |
| [Nationwide antimicrobial susceptibility survey of Neisseria gonorrhoeae isolates in Japan]. | In a nationwide antimicrobial susceptibility survey of 494 Nesseria gonorrhoeae isolates collected from February 2008 to December 2009 in 3 regions of Japan, 112 (22.7%) were collected from western Japan (Kinki, Chugoku, Shikoku, and Kyushu), 277 (56.1%) from mid-eastern Japan (Kanto), and 105 (21.1%) from eastern Japan (Tokai, Hokuriku, Koushinetsu, Tohoku, and Hokkaido). Resistance to ciprofloxacin (CPFX) was 72.8%, to penicillin G (PCG) 19.8%, and to tetracycline (TC) 18.2%. Intermediate resi ... | 2011 | 21861439 |
| the utility and cost of chlamydia trachomatis and neisseria gonorrhoeae screening of a male infertility population. | objective: to determine the utility and cost of chlamydia trachomatis (ct) and neisseria gonorrhoeae (ng) screening in infertile males. design: cohort study. setting: canadian tertiary-level male infertility clinic and university-affiliated laboratories. patient(s): 5,588 male infertility patients. intervention(s): ct and ng testing on 8,972 urine and semen samples. main outcome measure(s): prevalence of ct and ng infection in infertile males versus general male population in canada over 8 years ... | 2011 | 22192351 |
| [meningitis and encephalitis in poland in 2009]. | in poland, 2 517 cases of neuroinfections were reported in 2009, of which 865 had bacterial aetiology, 1 244--viral aetiology, and 408-- other or unknown origin. the etiological agent was determined in 493 (57%) cases of bacterial neuroinfections. among them neisseria meningitidis was found in 190 cases, haemophilus influenzae type b (hib) in 13 cases and streptococcus pneumoniae in 163 cases. an increasing trend in pneumococcal infections incidence has been observed since 2005, and a substantia ... | 2011 | 21913462 |
| elevated soluble urokinase receptor values in csf, age and bacterial meningitis infection are independent and additive risk factors of fatal outcome. | the aim of the present study was to evaluate the potential role of cerebrospinal fluid soluble urokinase receptor (supar) level, infection and age as risk factors for fatal outcome in patients suspected of having meningitis and/or bacteraemia on admission to hospital. a total of 545 cerebrospinal fluid samples from patients with clinically suspected meningitis were sent to the hellenic national meningitis reference laboratory. ten of 545 (1.83%) patients died. analysis by receiver operating char ... | 2011 | 21971819 |
| [severe bacterial infections and sudden death in children less than 4-years-old: 3 case reports.] | infectious factors contribute to sudden infant death in about 1 case out of 3. we report 3 children less than 4-years-old who died suddenly of bacterial infection due to neisseria meningitidis, streptococcus pneumoniae, and staphylococcus aureus. the bacteria were isolated from central and peripheral samples. a bacterial origin should be sought in all cases of sudden death in children. when a bacterial origin is confirmed, the question of immunodeficiency should be raised. | 2011 | 22209380 |
| evaluation of the subgingival microbiota of alcoholic and non-alcoholic individuals. | the aim of this study was to evaluate the composition of the subgingival microbiota of alcoholic and non-alcoholic individuals. | 2011 | 21864643 |
| evaluation of predominant neisseria gonorrhoeae strain types and its correlation with fluoroquinolone resistance in pakistan. | to study the predominant neisseria gonorrhoeae strain types in pakistan and to evaluate their correlation with fluoroquinolone resistance. | 2011 | 22204176 |
| pasteurella multocida cmp-sialic acid synthetase and mutants of neisseria meningitidis cmp-sialic acid synthetase with improved substrate promiscuity. | cytidine 5'-monophosphate (cmp)-sialic acid synthetases (csss) catalyze the formation of cmp-sialic acid from ctp and sialic acid, a key step for sialyltransferase-catalyzed biosynthesis of sialic acid-containing oligosaccharides and glycoconjugates. more than 50 different sialic acid forms have been identified in nature. to facilitate the enzymatic synthesis of sialosides with diverse naturally occurring sialic acid forms and their non-natural derivatives, cmp-sialic acid synthetases with promi ... | 2011 | 21968653 |
| mining virulence genes using metagenomics. | when a bacterial genome is compared to the metagenome of an environment it inhabits, most genes recruit at high sequence identity. in free-living bacteria (for instance marine bacteria compared against the ocean metagenome) certain genomic regions are totally absent in recruitment plots, representing therefore genes unique to individual bacterial isolates. we show that these metagenomic islands (mis) are also visible in bacteria living in human hosts when their genomes are compared to sequences ... | 2011 | 22039404 |
| a bacterial prib with weak single-stranded dna binding activity can stimulate the dna unwinding activity of its cognate pria helicase. | bacterial dna replication restart pathways facilitate reinitiation of dna replication following disruptive encounters of a replisome with dna damage, thereby allowing complete and faithful duplication of the genome. in neisseria gonorrhoeae, the primosome proteins that catalyze dna replication restart differ from the well-studied primosome proteins of e. coli with respect to the number of proteins involved and the affinities of their physical interactions: the pria:prib interaction is weak in e. ... | 2011 | 21861872 |
| influence of substrate modification and c-terminal truncation on the active site structure of substrate-bound heme oxygenase from neisseriae meningitidis. a 1h nmr study. | heme oxygenase (ho), from the pathogenic bacterium n. meningitidis(nmho), which secures host iron, shares many properties with mammalian hos but also exhibits some key differences. the crystal structure appears more compact, and the crystal-undetected c-terminus interacts with substrate in solution. the unique nature of substrate-protein, specifically pyrrole-i/ii-helix-2, peripheral interactions in nmho are probed by 2d (1)h nmr to reveal unique structural features controlling substrate orienta ... | 2011 | 21870860 |
| the epidemiology of human papillomavirus infection in hiv-positive and hiv-negative high-risk women in kigali, rwanda. | abstract: | 2011 | 22136570 |
| phenotypic and molecular characterization of serogroup c neisseria meningitidis associated with an outbreak in bahia, brazil. | objective: to characterize meningococcal strains isolated from five cases of meningococcal disease (md) associated with an outbreak in trancoso - ba, occurred in october 2009. all cases, with the exception of a 39-year-old male, attended a dance party with approximately 1000 youngsters in a rural site. materials and methods: the epidemiological investigation was conducted by the epidemiological surveillance service of bahia state. meningococcal strains were characterized at adolfo lutz institute ... | 2011 | 22078548 |
| characterization of profile of multidrug-resistant neisseria gonorrhoeae using old and new definitions in india over a decade: 2000-2009. | the burden of "multidrug-resistant neisseria gonorrhoeae" (mdr-ng) was high considering the old definition (26.0%). according to the new definitions, no strain was mdr or extensively drug-resistant. the emergence of resistance to ceftriaxone and cefixime will lead to detection of mdr-ng and may be extensively drug-resistant ng, even according to the new definition. | 2011 | 21992984 |
| characterization of a large genomic deletion in four irish families with c7 deficiency. | inherited deficiency of the seventh complement component (c7) is associated with increased susceptibility to neisseria meningitidis infections. the disease is rare in most western countries. here we report new investigations of a large, but incompletely characterized genomic deletion of exons 8 and 9 [c.739-?_1093+?del], previously identified in three unrelated irish families with c7 deficiency. we have analysed dna from one individual, who is homozygous for the deletion, by pcr using primers pr ... | 2011 | 22206826 |
| carriage of neisseria meningitidis in europe: a review of studies undertaken in the region. | meningococcal conjugate vaccines induce herd protection by preventing nasopharyngeal meningococcal acquisition, which is a prerequisite for invasive disease. thus, meningococcal carriage epidemiology is important in understanding relationships between carriage and disease. a literature search traced information on meningococcal carriage in 27 eu countries. meningococcal carriage prevalence differed within and between countries, varying across age groups, serogroup distribution and over time. car ... | 2011 | 21905785 |
| implementation and impact of a meningococcal c conjugate vaccination program in 13- to 25-year-old individuals in galicia, spain. | background: in response to increased case numbers of meningococcal group c disease, catch-up vaccination strategies have been shown to be successful. this paper describes the results of a repeat vaccination program in galicia, spain, and the strategy used for it. methods and results: three vaccination waves were performed: first, in 1996/1997 with a meningococcal group a and c polysaccharide vaccine in individuals aged 18 months to 19 years; second, in 2000 with a conjugate serogroup c polysacc ... | 2011 | 21957332 |
| effects of multi-walled carbon nanotubes (mwcnt) under neisseria meningitidis transformation process. | this study aimed at verifying the action of multi-walled carbon nanotubes (mwcnt) under the naturally transformable neisseria meningitidis against two different dna obtained from isogenic mutants of this microorganism, an important pathogen implicated in the genetic horizontal transfer of dna, causing the escape of the principal vaccination measured worldwide by the capsular switching process. | 2011 | 22088149 |
| identification of a conserved protein involved in anaerobic unsaturated fatty acid synthesis in neiserria gonorrhoeae: implications for facultative and obligate anaerobes that lack faba. | transcriptome analysis of the facultative anaerobe, neisseria gonorrhoeae, revealed that many genes of unknown function were induced under anaerobic conditions. mutation of one such gene, ngo1024, encoding a protein belonging to the 2-nitropropane dioxygenase-like superfamily of proteins, was found to result in an inability of gonococci to grow anaerobically. anaerobic growth of an ng1024 mutant was restored upon supplementation with unsaturated fatty acids (ufa), but not with the saturated fatt ... | 2011 | 21895795 |
| the prevalence of chlamydia trachomatis and neisseria gonorrhoeae infections among men with urethritis in kuwait. | chlamydial non-gonococcal urethritis and gonorrhoea are the most common sexually transmitted bacterial infections worldwide. data on these infections are scanty in the islamic world, especially kuwait. the objective of this study was to establish the prevalence of chlamydia trachomatis and neisseria gonorrhoeae among men with symptomatic urethritis in kuwait. | 2011 | 22000844 |
| hiv/std pattern and its associated risk factors among male std clinic attendees in china: a foci for hiv intervention. | abstract: | 2011 | 22200257 |
| a novel mechanism of high-level, broad-spectrum antibiotic resistance caused by a single base pair change in neisseria gonorrhoeae. | the mtrc-mtrd-mtre multidrug efflux pump of neisseria gonorrhoeae confers resistance to a diverse array of antimicrobial agents by transporting these toxic compounds out of the gonococcus. frequently in gonococcal strains, the expression of the mtrcde operon is differentially regulated by both a repressor, mtrr, and an activator, mtra. the mtrr gene lies 250 bp upstream of and is transcribed divergently from the mtrcde operon. previous research has shown that mutations in the mtrr coding region ... | 2011 | 21933917 |
| antibiotics for preventing meningococcal infections. | meningococcal disease is a contagious bacterial infection caused by neisseria meningitidis (n. meningitidis). household contacts have the highest risk of contracting the disease during the first week of a case being detected. prophylaxis is considered for close contacts of people with a meningococcal infection and populations with known high carriage rates. | 2011 | 21833949 |
| general metabolism of laribacter hongkongensis: a genome-wide analysis. | 2011 | 21711917 | |
| Deletion of one nucleotide within the homonucleotide tract present in the hsdS gene alters the DNA sequence specificity of type I restriction-modification system NgoAV. | As a result of a frameshift mutation, the hsdS locus of the NgoAV type IC restriction and modification (RM) system comprises two genes, hsdS(NgoAV1) and hsdS(NgoAV2). The specificity subunit, HsdS(NgoAV), the product of the hsdS(NgoAV1) gene, is a naturally truncated form of an archetypal specificity subunit (208 N-terminal amino acids instead of 410). The presence of a homonucleotide tract of seven guanines (poly[G]) at the 3' end of the hsdS(NgoAV1) gene makes the NgoAV system a strong candida ... | 2011 | 21984785 |
| Vaccines of the future. | Vaccines of the future can be divided into three broad groups, namely those of the near future (<10 years); the medium-term future (10-19 years); and the long-term future (20-50 years). For the near future, there is some "low hanging fruit" which is clearly on the horizon, such as a Vi-conjugate vaccine for typhoid or a protein-based vaccine for Neisseria meningitidis serogroup B. Just slightly more distant will be vaccines for shigellosis and a common protein vaccine for Streptococcus pneumonia ... | 2011 | 22185835 |
| Neisseria gonorrhoeae NspA induces specific bactericidal and opsonic antibodies in mice. | Neisseria gonorrhoeae surface protein A (NspA) is a highly conserved gonococcal antigen. To explore the potential of NspA in vaccine development against gonorrhea, BALB/c mice were immunized with pcNspA containing the NspA gene from N. gonorrhoeae strain WHO-A via intramuscular (i.m.) injection, intranasal (i.n.) immunization, or intravaginal (i.vag.) immunization. Following the last DNA immunization, mice were boosted with recombinant NspA (rNspA). Enzyme-linked immunosorbent assays (ELISAs) in ... | 2011 | 21918113 |
| chlamydia trachomatis prevalence and risk behaviors in parturient women aged 15 to 24 in brazil. | chlamydia trachomatis (ct) is a sexually transmitted infection having repercussions on reproductive health and impact on the foetus. our goal was to estimate the prevalence of and risk factors for ct in young parturient women in brazil. | 2011 | 21934572 |
| Universal protein vaccines against Neisseria meningitidis serogroup B, Streptococcus pneumoniae and influenza. | Traditional vaccines can only be really effective when the antigenic diversity or variability of the micro-organisms in the same bacterial species or viral family is no more than marginally different, and when protection is mainly dependent from antibody-mediated immunity. One solution is to identify the antigens common to all of the strains in a species or family that are significantly capable of eliciting inactivating antibodies. A number of attempts have recently been made to develop vaccines ... | 2011 | 21865879 |
| comparative long-term adverse effects elicited by invasive group b and c meningococcal infections. | given the identity between neisseria meningitidis serogroup b (menb) capsular polysaccharide (polysialic acid; psa) and psa found on neural cell adhesion molecules, it has been proposed that infection with menb or vaccination with psa may be associated with subsequent autoimmune or neurological disease. | 2011 | 21946191 |
| Invasive bacterial infections following influenza: a time-series analysis in Montréal, Canada, 1996-2008. | Please cite this paper as: Allard et al. (2011) Invasive bacterial infections following influenza: a time-series analysis in Montréal, Canada, 1996-2008. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750-2659.2011.00297.x. Background Shared seasonal patterns, such as between influenza and some respiratory bacterial infections, can create associations between phenomena not causally related. Objectives To estimate the association of influenza with subsequent bacterial infections after ... | 2011 | 21985083 |
| clinical validation of multiplex real-time pcr assays for detection of bacterial meningitis pathogens. | neisseria meningitidis (nm), haemophilus influenzae (hi), and streptococcus pneumoniae (sp) are important causes of meningitis and other infections, and rapid, sensitive, and specific laboratory assays are critical for effective public health interventions. singleplex real-time pcr assays have been developed to detect nm ctra, hi hpd and sp lyta, and serogroup-specific genes in the cap locus for nm serogroups a, b, c, w135, x and y. however, the assay sensitivity for serogroups b, w135 and y is ... | 2011 | 22170919 |
| introducing shear stress in the study of bacterial adhesion. | during bacterial infections a sequence of interactions occur between the pathogen and its host. bacterial adhesion to the host cell surface is often the initial and determining step of the pathogenesis. although experimentally adhesion is mostly studied in static conditions adhesion actually takes place in the presence of flowing liquid. first encounters between bacteria and their host often occur at the mucosal level, mouth, lung, gut, eye, etc. where mucus flows along the surface of epithelial ... | 2011 | 21912368 |
| structural investigation of the thermostability and product specificity of amylosucrase from the bacterium deinococcus geothermalis. | amylosucrases are sucrose-utilizing α-transglucosidases that naturally catalyze the synthesis of α-glucans, linked exclusively through α-1,4 linkages. side-products and in particular sucrose isomers such as turanose and trehalulose are also produced by these enzymes. here, we report the first structural and biophysical characterization of the most thermostable amylosucrase identified so far, the amylosucrase from deinoccocus geothermalis (dgas). the 3d-structure revealed a homodimeric quaterna ... | 2011 | 22210773 |
| Structure and mechanism of the lipooligosaccharide sialyltransferase from Neisseria meningitidis. | The first x-ray crystallographic structure of a CAZY family-52 glycosyltransferase, that of the membrane associated a2,3/a2,6 lipooligosaccharide sialyltransferase from Neisseria meningitidis serotype L1 (NST), has been solved to 1.95 Å resolution. The structure of NST adopts a GT-B-fold common with other glycosyltransferase (GT) families but exhibits a novel domain swap of the N-terminal 130 residues to create a functional homodimeric form not observed in any other class to date. The domain swa ... | 2011 | 21880735 |
| Trends in antimicrobial resistance in Neisseria gonorrhoeae isolated in Canada: 2000-2009. | Canada conducts surveillance of penicillin, tetracycline, erythromycin, spectinomycin, ciprofloxacin, cefixime, and ceftriaxone susceptibilities in Neisseria gonorrhoeae isolates to support development of national treatment guidelines for sexually transmitted infections. | 2011 | 21934558 |
| Neisseria meningitidis induces platelet inhibition and increases vascular endothelial permeability via nitric oxide regulated pathways. | Despite antibiotic therapy, infections with Neisseria meningitidis still demonstrate a high rate of morbidity and mortality even in developed countries. The fulminant septicaemic course, named Waterhouse-Friderichsen syndrome, with massive haemorrhage into the adrenal glands and widespread petechial bleeding suggest pathophysiological inhibition of platelet function. Our data show that N. meningitidis produces the important physiological platelet inhibitor and cardiovascular signalling molec ... | 2011 | 22072136 |
| correlation between serum bactericidal activity against neisseria meningitidis serogroups a, c, w-135 and y measured using human versus rabbit serum as the complement source. | the surrogate of protection against invasive meningococcal disease is the presence of serum bactericidal activity (sba) at a titer ≥4 in an assay using human serum as the complement source (hsba). however, for various practical and logistical reasons, many meningococcal vaccines in use today were licensed based on a modified sba assay that used baby rabbit serum as the complement source (rsba). to assess the strength of correlation between the two assay systems for serogroups a, c, w-135 and y, ... | 2011 | 22075087 |
| the first simultaneous study of the prevalence of neisseria gonorrhoeae and chlamydia trachomatis infections by polymerase chain reaction and standard methods. | neisseria gonorrhoeae and chlamydia trachomatis are important sexually transmissible pathogens. the aim of the present study was to determine their prevalence in symptomatic and asymptomatic people. | 2011 | 21851790 |
| [penicillinase production by neisseria gonorrhoeae strains isolated from the patients of dermatology and wenerology clinic, warsaw medical university in 2006 - 2009]. | in recent years the resistance of neisseria gonorrhoeae to antibiotics is increasing in many countries. the aim of the study was to investigate penicillinase production by neisseria gonorrhoeae strains isolated from the patients of clinic of dermatology and wenerology wum in a period between 2006 - 2009. we cultured the bacteria on roiron medium and we used the iodometric test or bbl cefinase discs to detect penicillinase. the enzyme was produced by 1,1% of 183, 0,9% of 111, 1,1% of 94 and 0% of ... | 2011 | 22184905 |
| applications of pcr (real-time and masstag) and enzyme-linked immunosorbent assay in diagnosis of respiratory infections and diarrheal illness among deployed u.s. military personnel during exercise balikatan 2009, philippines. | laboratory-based surveillance for diarrheal and respiratory illness was conducted at the 2009 republic of the philippines-united states balikatan exercise to determine the presence of specific pathogens endemic in the locations where the military exercises were conducted. ten stool and 6 respiratory specimens were obtained from individuals meeting case definitions for diarrhea or respiratory illness. stool specimens were frozen in dry ice and remotely tested using enzyme-linked immunosorbent ass ... | 2011 | 22128641 |
| [anti-cytokine activity of microorganisms]. | development of a method of determination of anti-cytokine activity (aca) of microorganisms, study of the prevalence and intensity of aca to pro- and anti-inflammatory cytokines in pathogenic and opportunistic bacteria. | 2011 | 21913393 |
| estimating the prevalence of gonococcal resistance to antimicrobials in england and wales. | gonorrhoea can rapidly develop resistance to antimicrobials and treatment options are becoming depleted. treatment guidelines require robust estimates of the prevalence of resistance but sentinel surveillance systems may not be representative nationally. | 2011 | 21917698 |
| influence of conserved and hypervariable genetic markers on genotyping circulating strains of neisseria gonorrhoeae. | presently there is no vaccine against neisseria gonorrhoeae and therefore accurate information on gonococcal transmission plays a crucial role for interventions designed to limit the spread of infections caused by this microorganism. we evaluated the impact of two different categories of genetic markers, (i) concatenated sequences of 10 housekeeping genes and (ii) hypervariable porb dna sequences, on the genetic relatedness and subsequently on genotyping analysis of this human pathogen. eighty g ... | 2011 | 22163288 |
| increasing trend of resistance to penicillin, tetracycline, and fluoroquinolone resistance in neisseria gonorrhoeae from pakistan (1992-2009). | emergence and spread of drug resistant neisseria gonorrhoeae is global concern. we evaluated trends of antimicrobial resistance in neisseria gonorrhoeae over years 1992-2009 in pakistan. resistance rates were compared between years (2007-2009) and (1992-2006). antimicrobial susceptibility testing was performed and interpreted according to clinical laboratory standards institute (clsi) criteria using the disk diffusion methodology against penicillin, ceftriaxone, tetracycline and ofloxacin. addit ... | 2011 | 21941568 |
| structural bioinformatics of neisseria meningitidis ld-carboxypeptidase: implications for substrate binding and specificity. | neisseria meningitidis, a gram negative bacterium, is the leading cause of bacterial meningitis and severe sepsis. neisseria meningitidis genome contains 2,160 predicted coding regions including 1,000 hypothetical genes. re-annotation of n. meningitidis hypothetical proteins identified nine putative peptidases. among them, the nmb1620 protein was annotated as ld-carboxypeptidase involved in peptidoglycan recycling. structural bioinformatics studies of nmb1620 protein using homology modeling and ... | 2011 | 21983752 |
| response to 'comparative performance of culture using swabs transported in amies medium and the aptima combo 2 nucleic acid amplification test in detection of neisseria gonorrhoeae from genital and extra-genital sites: a retrospective study' | 2011 | 22194583 | |
| penicillin binding proteins as danger signals: meningococcal penicillin binding protein 2 activates dendritic cells through toll-like receptor 4. | neisseria meningitidis is a human pathogen responsible for life-threatening inflammatory diseases. meningococcal penicillin-binding proteins (pbps) and particularly pbp2 are involved in bacterial resistance to β-lactams. here we describe a novel function for pbp2 that activates human and mouse dendritic cells (dc) in a time and dose-dependent manner. pbp2 induces mhc ii (logec50 = 4.7 µg/ml ± 0.1), cd80 (logec50 = 4.88 µg/ml ± 0.15) and cd86 (logec50 = 5.36 µg/ml ± 0.1). this effect was abolishe ... | 2011 | 22046231 |
| first neisseria gonorrhoeae strain with resistance to cefixime causing gonorrhoea treatment failure in austria, 2011. | we describe the first cefixime-resistant neisseria gonorrhoeae strain in austria that caused treatment failure.it follows the first five cases in europe of cefixime treatment failure, reported in norway in 2010 and the united kingdom in 2011. effective treatment of gonorrhoea is crucial for public health control and, at present, requires substantially enhanced awareness, more frequent test-of-cure, interaction with experts after therapeutic failure, tracing and therapy of contacts, and surveilla ... | 2011 | 22085601 |
| interventions to prevent sexually transmitted infections, including hiv infection. | the centers for disease control and prevention (cdc) sexually transmitted disease (std) treatment guidelines were last updated in 2006. to update the "clinical guide to prevention services" section of the 2010 cdc std treatment guidelines, we reviewed the recent science with reference to interventions designed to prevent acquisition of stds, including human immunodeficiency virus (hiv) infection. major interval developments include (1) licensure and uptake of immunization against genital human p ... | 2011 | 22080271 |
| patterns of chlamydia/gonorrhea positivity among voluntarily screened new york city public high school students. | chlamydia trachomatis (ct) and neisseria gonorrhoeae (gc) are common sexually transmitted infections that disproportionately affect adolescents. annual screening for ct for sexually active female adolescents is recommended. in 2006, new york city began conducting ct/gc education, screening, and treatment in public high schools. we examine 3-year programmatic outcomes and the relationship between sexual activity, screening, and ct/gc positivity. | 2011 | 21856516 |
| acute bacterial meningitis in infants and children: epidemiology and management. | acute bacterial meningitis (abm) continues to be associated with high mortality and morbidity, despite advances in antimicrobial therapy. the causative organism varies with age, immune function, immunization status, and geographic region, and empiric therapy for meningitis is based on these factors. haemophilus influenzae type b (hib), streptococcus pneumoniae, and neisseria meningitidis cause the majority of cases of abm.disease epidemiology is changing rapidly due to immunization practices and ... | 2011 | 21960054 |
| evaluation of the siemens versant® ct/gc dna 1.0 assay (kpcr) for detection of chlamydia trachomatis and neisseria gonorrhoeae. | the siemens versant kpcr system is an automated system which combines extraction of nucleic acids from 96 samples with subsequent real-time pcr. the versant ct/gc dna 1.0 (kpcr) assay detects chlamydia trachomatis (ct) and neisseria gonorrhoeae (gc) in a multiplex real-time pcr on this system. we compared this assay with the bd probete™ et system (pt) and the roche cobas amplicor (ca). three different sets of samples were tested in the kpcr: pt pre-treated samples, prospectively collected urine ... | 2011 | 21925547 |
| vascular colonization by neisseria meningitidis. | bacterial infection of human vasculature can lead to unregulated systemic activation of coagulation and innate immunity and rapidly becomes life threatening. neisseria meningitidis is a vascular pathogen that causes fatal sepsis and meningitis. post-mortem histological analysis of tissues from individuals infected with n. meningitidis show large bacterial aggregates in close association with the vascular wall of small vessels. the ability of this bacterium to colonize blood vessel endothelium is ... | 2011 | 22185907 |
| enhanced bacteremia in human factor h transgenic rats infected by neisseria meningitidis. | neisseria meningitidis binds the complement down-regulating protein, factor h (fh), which enables the organism to evade host defenses. two fh ligands, fhbp and nspa, are known to bind specifically to human fh. we developed a human fh transgenic infant rat model to investigate the effect of human fh on meningococcal bacteremia. 18 hours after ip challenge with 560 cfu of group b strain h44/76, all 19 human fh-positive rats had positive blood cultures compared with 0 of 7 human fh-negative control ... | 2011 | 22104107 |
| neisserial correia repeat-enclosed elements do not influence the transcription of pil genes in neisseria gonorrhoeae and neisseria meningitidis. | two human-specific neisserial pathogens, neisseria gonorrhoeae and neisseria meningitidis, require the expression of type iv pili (tfp) for initial attachment to the host during infection. however, the mechanisms controlling the assembly and functionality of tfp are poorly understood. it is known that the gonococcal pile gene, encoding the major subunit, is positively regulated by ihf, a multifunctional dna binding protein. a neisserial specific repetitive dna sequence, termed the correia repeat ... | 2011 | 21856854 |
| nonhuman primate models used to study pelvic inflammatory disease caused by chlamydia trachomatis. | pelvic inflammatory disease (pid) is a global health concern that is associated with significant morbidity and is a major cause of infertility. throughout history animals have been used for anatomical studies and later as models of human disease. in particular, nonhuman primates (nhps) have permitted investigations of human disease in a biologically, physiologically, and anatomically similar system. the use of nhps as human pid models has led to a greater understanding of the primary microorgani ... | 2011 | 21869858 |
| epidemiology of invasive meningococcal disease in austria 2010. | neisseria meningitidis is a leading cause of meningitis and sepsis worldwide. thus, monitoring the epidemiology of invasive meningococcal disease (imd) is an important public health measure. the national reference centre for meningococci at the austrian agency for health and food safety is operating the laboratory-based surveillance database. | 2011 | 21935643 |
| immediate outcomes of bacterial meningitis in childhood may benefit from slow initial β-lactam infusion and oral paracetamol. | evaluation of: pelkonen t, roine i, cruzeiro ml, pitkaranta a, kataja m, peltola h. slow initial β-lactam infusion and oral paracetamol to treat childhood bacterial meningitis: a randomised, controlled trial. lancet infect. dis. 11(8), 613-621 (2011). acute bacterial meningitis is a medical emergency that requires prompt management. despite effective antibiotic and adjunctive therapies, mortality is still unacceptably high in acute bacterial meningitis in children as this mortality did not subst ... | 2011 | 22004030 |
| cleavage of von willebrand factor by granzyme m destroys its factor viii binding capacity. | von willebrand factor (vwf) is a pro-hemostatic multimeric plasma protein that promotes platelet aggregation and stabilizes coagulation factor viii (fviii) in plasma. the metalloproteinase adamts13 regulates the platelet aggregation function of vwf via proteolysis. severe deficiency of adamts13 is associated with thrombotic thrombocytopenic purpura, but does not always correlate with its clinical course. therefore, other proteases could also be important in regulating vwf activity. in the presen ... | 2011 | 21909423 |
| a sulfonium cation intermediate in the mechanism of methionine sulfoxide reductase b: a dft study. | the hybrid density functional theory method b3lyp in combination with three systematically larger active site models has been used to investigate the substrate binding and catalytic mechanism by which neisseria gonorrhoeae methionine sulfoxide reductase b (msrb) reduces methionine-r-sulfoxide (met-r-so) to methionine. the first step in the overall mechanism is nucleophilic attack of an active site thiolate at the sulfur of met-r-so to form an enzyme-substrate sulfurane. this occurs with concomit ... | 2011 | 21721538 |
| meningococcal disease: the advances and challenges of meningococcal disease prevention. | vaccination as a means to prevent meningococcal disease caused by neisseria meningitidis is critical given the abrupt onset and rapid progression of this disease. five serogroups-a, b, c, w-135, and y-are responsible for the majority of cases. in developed countries, infants have the greatest risk of disease, with a smaller secondary peak observed in late adolescence. vaccines utilizing the polysaccharide capsule are poorly immunogenic in young children but can reduce the incidence of meningococ ... | 2011 | 21832882 |
| combining carbochips and mass spectrometry to study the donor specificity for the neisseria meningitidis +¦1,3-n-acetylglucosaminyltransferase lgta. | a library of 11 udp-n-acetylglucosamine analogs were rapidly screened for their activities as donors for the neisseriameningitidis +¦1,3-n-acetylglucosaminyltransferase (lgta) by direct on-chip reaction and detection with samdi-tof mass spectrometry. six of the analogs were active in this assay and were analyzed by samdi to characterize the kinetics toward lgta. the analysis revealed that substitutions on c-2, c-4, and c-6 affect the activity of the donors, with bulky groups at these positions d ... | 2011 | 21704524 |
| TLR2 Activation Enhances HIV Nuclear Import and Infection through T Cell Activation-Independent and -Dependent Pathways. | TLR2 activation plays a crucial role in Neisseria gonorrheae-mediated enhancement of HIV infection of resting CD4(+) T cells. We examined signaling pathways involved in the HIV enhancing effect of TLR2. TLR2 but not IL-2 signals promoted HIV nuclear import; however, both signals were required for the maximal enhancing effect. Although TLR2 signaling could not activate T cells, it increased IL-2-induced T cell activation. Cyclosporin A and IkBa inhibitor blocked TLR2-mediated enhancement of HIV i ... | 2011 | 22210918 |
| Population structure in the Neisseria, and the biological significance of fuzzy species. | Phenotypic and genetic variation in bacteria can take bewilderingly complex forms even within a single genus. One of the most intriguing examples of this is the genus Neisseria, which comprises both pathogens and commensals colonizing a variety of body sites and host species, and causing a range of disease. Complex relatedness among both named species and previously identified lineages of Neisseria makes it challenging to study their evolution. Using the largest publicly available collection of ... | 2011 | 22072450 |
| microbiological findings in endometrial specimen: our experience. | purpose: collection of an endometrial specimen for investigating infectious agents in the endometrial cavity is an invasive technique that is at times difficult and painful. in order to avoid the need for endometrial sampling in the cases of suspected or evident endometrial pathology, the aim of this study is to investigate the reliability of cervical cultures for detecting infectious agents present at the endometrial level, comparing the results between cervical cultures a ... | 2011 | 22113463 |
| antibiotic-resistant phenotypes and genotypes of neisseria gonorrhoeae isolates in japan: identification of strain clusters with multidrug-resistant phenotypes. | : to determine the antibiotic susceptibility and the genotype distributions of n. gonorrhoeae isolates in fukuoka, japan, and to evaluate the specific associations between genotypes and antibiotic resistance. | 2011 | 21844744 |
| cephalosporin susceptibility among neisseria gonorrhoeae isolates--united states, 2000-2010. | neisseria gonorrhoeae is a major cause of pelvic inflammatory disease, ectopic pregnancy, and infertility, and it can facilitate human immunodeficiency virus (hiv) transmission. emergence of gonococcal resistance to penicillin and tetracycline occurred during the 1970s and became widespread during the early 1980s. more recently, resistance to fluoroquinolones developed. resistance was documented first in asia, then emerged in the united states in hawaii followed by other western states. it then ... | 2011 | 21734634 |
| withdrawn: conjugate vaccines for preventing meningococcal c meningitis and septicaemia. | meningococcal polysaccharide (mpls) vaccines protect against serogroup c disease, but do not produce an immune response in infants less than two years of age. this limitation can be overcome by linking c polysaccharide to carrier proteins ('conjugating'), to create meningococcal serogroup c conjugate (mcc) vaccines. in the absence of trial data, the immune response to vaccination has been considered to be a reasonable surrogate for vaccine protection. | 2011 | 21735387 |
| n. elongata produces type iv pili that mediate interspecies gene transfer with n. gonorrhoeae. | the genus neisseria contains at least eight commensal and two pathogenic species. according to the neisseria phylogenetic tree, commensals are basal to the pathogens. n. elongata, which is at the opposite end of the tree from n. gonorrhoeae, has been observed to be fimbriated, and these fimbriae are correlated with genetic competence in this organism. we tested the hypothesis that the fimbriae of n. elongata are type iv pili (tfp), and that tfp functions in genetic competence. we provide evidenc ... | 2011 | 21731720 |
| diversity of factor h-binding protein in neisseria meningitidis carriage isolates. | several meningococcal vaccines under development for prevention of serogroup b disease target the factor h-binding protein (fhbp), an immunogenic lipoprotein expressed on the surface of neisseria meningitidis. based upon sequence and phylogenetic analyses, fhbp can be classified into 3 protein variants (1, 2 or 3) or 2 subfamilies (a or b). the potential effect of fhbp-containing vaccines on meningococcal carriage is not known. we determined the diversity of fhbp among a population of carriage i ... | 2011 | 21704667 |
| resistance to azithromycin of neisseria gonorrhoeae isolates from 2 cities in china. | : antimicrobial resistance in neisseria gonorrhoeae (ng) is a public health concern globally, and azithromycin-resistant ng isolates have been identified in many countries. this study aimed to investigate azithromycin susceptibility in ng and to genetically characterize the azithromycin-resistant isolates identified in 2 cities in china. | 2011 | 21844727 |
| experimental gonococcal infection in male volunteers: cumulative experience with neisseria gonorrhoeae strains fa1090 and ms11mkc. | experimental infection of male volunteers with neisseria gonorrhoeae is safe and reproduces the clinical features of naturally acquired gonococcal urethritis. human inoculation studies have helped define the natural history of experimental infection with two well-characterized strains of n. gonorrhoeae, fa1090 and ms11mkc. the human model has proved useful for testing the importance of putative gonococcal virulence factors for urethral infection in men. studies with isogenic mutants have improve ... | 2011 | 21734909 |
| antimicrobial susceptibility of bacterial isolates from community acquired infections in sub-saharan africa and asian low and middle income countries. | objectiveôçé antimicrobial resistance has arisen across the globe in both nosocomial and community settings as a consequence of widespread antibiotic consumption. poor availability of laboratory diagnosis means that resistance frequently goes unrecognised and may only be detected as clinical treatment failure. in this review, we provide an overview of the reported susceptibility of common community acquired bacterial pathogens in sub-saharan africa and asia to the antibiotics that are most widel ... | 2011 | 21707879 |
| review and international recommendation of methods for typing neisseria gonorrhoeae isolates and their implications for improved knowledge of gonococcal epidemiology, treatment, and biology. | gonorrhea, which may become untreatable due to multiple resistance to available antibiotics, remains a public health problem worldwide. precise methods for typing neisseria gonorrhoeae, together with epidemiological information, are crucial for an enhanced understanding regarding issues involving epidemiology, test of cure and contact tracing, identifying core groups and risk behaviors, and recommending effective antimicrobial treatment, control, and preventive measures. this review evaluates me ... | 2011 | 21734242 |
| [cloning and prokaryotic expression of the outer membrane protein gene porb of neisseria gonorrhoeae]. | to construct a fused expression vector of the outer membrane protein gene porb of neisseria gonorrhoeae, express the fusion protein in the prokaryotic system, and obtain a gene recombination protein, for the purpose of preparing the ground for further research on the pathopoiesis and immune protective response of porb. | 2011 | 21823339 |
| computational electrophysiology: the molecular dynamics of ion channel permeation and selectivity in atomistic detail. | presently, most simulations of ion channel function rely upon nonatomistic brownian dynamics calculations, indirect interpretation of energy maps, or application of external electric fields. we present a computational method to directly simulate ion flux through membrane channels based on biologically realistic electrochemical gradients. in close analogy to single-channel electrophysiology, physiologically and experimentally relevant timescales are achieved. we apply our method to the bacterial ... | 2011 | 21843471 |
| development of an automated, high-throughput bactericidal assay that measures cellular respiration as a survival readout for neisseria meningitidis. | complement-mediated bactericidal activity has long been regarded as the serological correlate of protective immunity against neisseria meningitidis. this was affirmed in 2005 at a who-sponsored meningococcal serology standardization workshop. the assay currently employed by most laboratories involves determining surviving bacterial colony counts on agar as a readout which is labor-intensive, time-consuming, and not amendable to rapid data analysis for clinical trials. consequently, there is an a ... | 2011 | 21715580 |
| a laboratory-based evaluation of the biostar optical immunoassay (oia) point-of-care test (poct) for diagnosing neisseria gonorrhoeae. | abstractthe development of gonococcal point-of-care tests (pocts) has been challenging due to the relatively monomorphic nature of the neisseria genus. the biostar optical immunoassay (oia) poct for diagnosing neisseria gonorrhoeae detects a specific epitope on the l7/l12 ribosomal protein, which reduces cross-reactivity with other neisseriae, and produces a highly specific test. a laboratory-based evaluation of this poct was performed to determine its analytical sensitivity and specificity. a p ... | 2011 | 21835969 |
| case-control study of ectopic pregnancies in myanmar: infectious etiological factors. | we studied the role of infections in ectopic pregnancy and the different methods of detecting chlamydia trachomatis infection using serology, cervical and tubal pcr assays, by using a hospital-based, case-control study conducted between november 2007 and september 2009. the sample size was 339 with 113 cases and 226 controls. the cases were women admitted for the management of ectopic pregnancy while the controls were women admitted for spontaneous miscarriage. both cases and controls were teste ... | 2011 | 21710857 |
| Neisseria gonorrhoeae-mediated inhibition of apoptotic signalling in polymorphonuclear leukocytes. | The human pathogen Neisseria gonorrhoeae recruits and interacts extensively with polymorphonuclear leukocytes (PMNs) during infection. N. gonorrhoeae is able to survive the bactericidal activity of these innate immune cells, and can actively modulate PMN functions in vitro. PMNs are short-lived cells which readily undergo apoptosis, thus the effect of N. gonorrhoeae infection on PMN survival has implications for whether PMNs might serve as an important site of bacterial replication during infect ... | 2011 | 21844239 |
| Positive nucleic acid amplification tests for Neisseria gonorrhoeae in young people tested as part of the National Chlamydia Screening Programme. | Little information is available on the prevalence of Neisseria gonorrhoeae (GC) infections outside genitourinary (GU) medicine clinics. A number of National Chlamydia Screening Programme areas now carry out simultaneous testing for Chlamydia trachomatis (CT) and GC in a single sample using nucleic acid amplification tests (NAATs). The aim of this study was to gather together data on dual testing from community settings. Information was collected from five programme areas. A total of 219,412 resu ... | 2011 | 21729959 |
| Computational electrophysiology: the molecular dynamics of ion channel permeation and selectivity in atomistic detail. | 2011 | 21843464 | |
| Antimicrobial resistance in Neisseria gonorrhoeae and Treponema pallidum: evolution, therapeutic challenges and the need to strengthen global surveillance. | 2011 | 22110154 | |
| the iron-repressed, arac-like regulator mper activates expression of feta in neisseria gonorrhoeae. | neisseria gonorrhoeae is an obligate human pathogen that causes the common sexually transmitted infection gonorrhea. gonococcal infections cause significant morbidity, particularly among women, as the organism ascends to the upper reproductive tract, resulting in pelvic inflammatory disease, ectopic pregnancy, and infertility. in the last few years, antibiotic resistance rates have risen dramatically, leading to severe restriction of treatment options for gonococcal disease. gonococcal infection ... | 2011 | 21947770 |
| Trends in antimicrobial resistance of Neisseria gonorrhoeae isolated from Korean patients from 2000 to 2006. | The recent emergence and spread of antimicrobial-resistant Neisseria gonorrhoeae has compromised treatment and control of gonorrhea. We determined recent trends in antimicrobial susceptibility of the isolates, analyzed recent use of antigonococcal agents, and investigated the relationship between fluoroquinolone nonsusceptibility and amino acid substitutions within the fluoroquinolone resistance-determining regions in Korea. | 2011 | 21992988 |
| genital and extra-genital screening for gonorrhoea using the bd probetec et system with an in-house pcr method targeting the pora pseudogene as confirmatory test. | diagnosing gonorrhoea from extra-genital as well as genital sites is important in managing this sexually transmitted disease. in this study we evaluated a screening procedure for neisseria gonorrhoeae (gc) from all sample sites in a low-prevalence setting. a total of 69,252 specimens submitted for chlamydia trachomatis testing were also examined for gc on the bd viper™ platform using the bd probetec et system. in order to avoid false-positive results all gc bd reactive samples were re-tested usi ... | 2011 | 22002460 |
| Repeating Low-Positive Nucleic Acid Amplification Test Results for Chlamydia trachomatis and Neisseria gonorrhoeae: Assessment of Current Practice in Selected California Public and Private-Sector Laboratories. | Routine repeat testing of specimens with a low-positive result for Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) by nucleic acid amplification test (NAAT) is not recommended (5) because 1) the majority of positive specimens that are negative on confirmatory testing are true positives, and 2) infected patients will go untreated.… | 2011 | 22116148 |
| eculizumab: a review of its use in paroxysmal nocturnal haemoglobinuria. | eculizumab is a humanized monoclonal antibody indicated for the treatment of paroxysmal nocturnal haemoglobinuria (pnh). it binds specifically and with high affinity to the complement protein c5, thereby preventing the formation of the terminal complement complex c5b-9, which mediates cell lysis. in patients with pnh, eculizumab inhibits terminal complement mediated intravascular haemolysis. in clinical trials of pnh patients, eculizumab reduced intravascular haemolysis compared with baseline an ... | 2011 | 22085388 |
| cefixime and ceftriaxone susceptibility of neisseria gonorrhoeae in italy from 2006 to 2010. | clin microbiol infect abstract: neisseria gonorrhoeae resistance to cephalosporins, the currently recommended treatment, and treatment failures with cefixime have been reported worldwide. the purposes of the present study were (i) to examine the susceptibility of n. gonorrhoeae isolates isolated in italy from 2006 through 2010 to cefixime (n = 293) taking into account both european committee on antimicrobial susceptibility testing (eucast) and clinical and laboratory standards institute (clsi) c ... | 2011 | 21966997 |
| Outbreak of Neisseria meningitidis C in workers at a large food-processing plant in Brazil: challenges of controlling disease spread to the larger community. | SUMMARYAn outbreak of meningococcal disease (MD) with severe morbidity and mortality was investigated in midwestern Brazil in order to identify control measures. A MD case was defined as isolation of Neisseria meningitidis, or detection of polysaccharide antigen in a sterile site, or presence of clinical purpura fulminans, or an epidemiological link with a laboratory-confirmed case-patient, between June and August 2008. In 8 out of 16 MD cases studied, serogroup C ST103 complex was identified. F ... | 2011 | 21875453 |
| human lipooligosaccharide igg that prevents endemic meningococcal disease recognizes an internal lacto-n-neotetraose structure. | antibodies that initiate complement-mediated killing of neisseria meningitidis as they enter the bloodstream from the oropharynx protect against disseminated disease. human iggs that bind the neisserial l7 lipooligosaccharide (los) are bactericidal for l3,7 and l2,4 meningococci in the presence of human complement. these strains share a lacto-n-neotetraose (nlc4) los α chain. we used a set of mutants that have successive saccharide deletions from the nlc4 α chain to characterize further the bind ... | 2011 | 22027827 |