Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| synthesis of copper ion incorporated horseradish peroxidase-based hybrid nanoflowers for enhanced catalytic activity and stability. | in this study, we report the preparation, catalytic activity and stability of a hybrid nanoflower (hnf) formed from horseradish peroxidase (hrp) enzyme and copper ions (cu(2+)). we studied the morphology of hnfs as a function of the concentrations of copper (cu(2+)) ions, chloride ions (cl(-)) and hrp enzyme, the ph of the buffer solution (phosphate buffered saline), and the temperature of the reaction. the effects of morphology on the catalytic activity and stability of hnfs were evaluated by o ... | 2015 | 25940219 |
| enzymatic assay to test diamines produced by vaginal bacteria. | an enzymatic assay was developed to determine the concentration of diamines (da) in clinical samples of vaginal fluids. putrescine and cadaverine are da produced by anaerobic bacteria and are typically present in the vaginal fluids of women with an abnormal microbiota, as occurs in bacterial vaginosis. the vaginal da (vada) assay is based on the enzyme diamine oxidase which reacts with putrescine and cadaverine to produce h2o2 in a quantitative manner. h2o2 concentration is measured spectrophoto ... | 2015 | 25938752 |
| microrna-triggered, cascaded and catalytic self-assembly of functional "dnazyme ferris wheel" nanostructures for highly sensitive colorimetric detection of cancer cells. | the construction of dna nanostructures with various sizes and shapes has significantly advanced during the past three decades, yet the application of these dna nanostructures for solving real problems is still in the early stage. on the basis of microrna-triggered, catalytic self-assembly formation of the functional "dnazyme ferris wheel" nanostructures, we show here a new signal amplification platform for highly sensitive, label-free and non-enzyme colorimetric detection of a small number of hu ... | 2015 | 25921227 |
| biochip array technology immunoassay performance and quantitative confirmation of designer piperazines for urine workplace drug testing. | designer piperazines are emerging novel psychoactive substances (nps) with few high-throughput screening methods for their identification. we evaluated a biochip array technology (bat) immunoassay for phenylpiperazines (pnp) and benzylpiperazines (bzp) and analyzed 20,017 randomly collected urine workplace specimens. immunoassay performance at recommended cutoffs was evaluated for pnpi (5 μg/l), pnpii (7.5 μg/l), and bzp (5 μg/l) antibodies. eight hundred forty positive and 206 randomly selected ... | 2015 | 25903022 |
| annexin v-directed enzyme prodrug therapy plus docetaxel for the targeted treatment of pancreatic cancer. | the bleak prognosis associated with pancreatic cancer (pdac) drives the need for the development of novel treatment methodologies. here, we evaluate the applicability of 3 enzyme prodrug therapies for pdac, which are simultaneously targeted to the tumor, tumor vasculature, and metastases via annexin v. in these therapies, annexin v is fused to an enzyme, creating a fusion protein that converts nontoxic drug precursors, prodrugs, into anticancer compounds while bound to the tumor, therefore mitig ... | 2015 | 25899647 |
| human alpha-fetal protein immunoassay using fluorescence suppression with fluorescent-bead/antibody conjugate and enzymatic reaction. | the aim of the study was to develop a simple and rapid immunoassay using fluorescent microbeads and enzyme-substrate reactions to measure alpha-fetal protein (afp) concentrations. we demonstrated the functionality of the fluorescent immunosensor using antibody-conjugated fluorescent latex beads (ab-flbs) and horseradish peroxidase (hrp) to catalyze a reaction, where the products would precipitate and suppress the fluorescence of ab-flbs. first, the ab-flbs were incubated with antigen, biotinylat ... | 2015 | 25897880 |
| cryoprotective ability of betaine-type metabolite analogs during freezing denaturation of enzymes. | to evaluate an analog library of betaine-type cellular metabolites, which are naturally found in polar fish for survival in subzero temperatures, for preventing denaturation of enzymes during freezing. | 2015 | 25893326 |
| cell-selective encapsulation in hydrogel sheaths via biospecific identification and biochemical cross-linking. | selective encapsulation of a particular cell population from heterogeneous cell populations has potential applications such as studies in cell-to-cell communication, regenerative medicine, and cell therapies. however, there are no versatile methods for realizing this. here we report a method based on biospecific identification of the target cells through antigen-antibody reaction and subsequent enzymatic hydrogel sheath formation on the cell surfaces by horseradish peroxidase (hrp). human hepato ... | 2015 | 25890746 |
| of the importance of a leaf: the ethnobotany of sarma in turkey and the balkans. | sarma - cooked leaves rolled around a filling made from rice and/or minced meat, possibly vegetables and seasoning plants - represents one of the most widespread feasting dishes of the middle eastern and south-eastern european cuisines. although cabbage and grape vine sarma is well-known worldwide, the use of alternative plant leaves remains largely unexplored. the aim of this research was to document all of the botanical taxa whose leaves are used for preparing sarma in the folk cuisines of tur ... | 2015 | 25890379 |
| edaravone combined with schwann cell transplantation may repair spinal cord injury in rats. | edaravone has been shown to delay neuronal apoptosis, thereby improving nerve function and the microenvironment after spinal cord injury. edaravone can provide a favorable environment for the treatment of spinal cord injury using schwann cell transplantation. this study used rat models of complete spinal cord transection at t9. six hours later, schwann cells were transplanted in the head and tail ends of the injury site. simultaneously, edaravone was injected through the caudal vein. eight weeks ... | 2015 | 25883621 |
| chemiluminescence-imaging detection of dna on a solid-phase membrane by using a peroxidase-labeled macromolecular probe. | we have developed a novel method for sensitive chemiluminescence (cl)-imaging detection of dna by using a macromolecular probe synthesized by attaching multiple molecules of horseradish peroxidase (hrp) and biotin in dextran backbone. the probe formed a macromolecular assembly by binding to streptavidin which specifically recognized biotinylated complementary dna, which was hybridized to a target dna on a solid-phase membrane. this methodology was applied to cl-imaging detection of a synthetic t ... | 2015 | 25882419 |
| measuring hydrogen peroxide due to water radiolysis using a modified horseradish peroxidase based biosensor as an alternative dosimetry method. | h2o2 generated during water radiolysis was measured electrochemically as an alternative dosimetry method. a biosensor was fabricated by immobilising modified horseradish peroxidase (hrp) on a glassy carbon electrode (gce) followed by evaluation of its analytical parameters. anthraquinone 2-carboxylic acid was used to modify hrp. to assess sensor performance, phosphate buffer solutions were irradiated with 0.510 gy of gamma ray emitted from (60)co. the results showed that this sensor can detect l ... | 2015 | 25880374 |
| morphometric and functional analysis of axonal regeneration after end-to-end and end-to-side neurorrhaphy in rats. | end-to-side neurorrhaphy is controversial in the literature and has sparked debate over its degree of recovery. in this study, nerve regeneration was assessed in rats after end-to-side neurorrhaphy by morphometric analysis, electromyography, electron microscopy, and retrograde horseradish peroxidase (hrp) and fluoro-gold (fg; fluorochrome inc., denver, colo.) transport and then compared to end-to-end neurorrhaphy and sham operation. | 2015 | 25878937 |
| a double-enhanced strip biosensor for the rapid and ultrasensitive detection of protein biomarkers. | combining the specific molecular recognition reaction between biotin and streptavidin with a dual-class signal amplification system, containing the unique optical properties of gold nanoparticles and the catalytic reaction of horseradish peroxidase, a double-enhanced strip biosensor was designed for the first time for a visual and rapid detection of protein biomarkers with good accuracy, especially for cea with a detection limit of 2.9 fg ml(-1). | 2015 | 25875055 |
| fluorescence in situ hybridization and sequential catalyzed reporter deposition (2c-fish) for the flow cytometric sorting of freshwater ultramicrobacteria. | flow cytometric sorting is a powerful tool to physically separate cells within mixed microbial communities. if combined with phylogenetic staining (fluorescence in situ hybridization, fish) it allows to specifically sort defined genotypic microbial populations from complex natural samples. however, the targeted enrichment of freshwater ultramicrobacteria, such as members of the ld12 clade of alphaproteobacteria (sar11-iiib), is still challenging. current fish protocols, even in combination with ... | 2015 | 25873914 |
| an enzyme-entrapped agarose gel for visualization of ischemia-induced l-glutamate fluxes in hippocampal slices in a flow system. | an agarose gel slip containing l-glutamate oxidase (gluox), horseradish peroxidase (hrp) and a dye da-64 is proposed as a tool for visualizing ischemia-induced l-glutamate release in hippocampal slices in a flow system. the agarose slip with a detection limit of 6.0 ± 0.8 μmol l(-1) for l-glutamate enabled us to visualize l-glutamate fluxes in a flow system. the leak of a dye from the agarose gel was negligible and a diffusion blur due to spreading of bindshedler's green (bg) within the gel was ... | 2015 | 25864676 |
| a plasmonic aptasensor for ultrasensitive detection of thrombin via arrested rolling circle amplification. | a sensitive signal generation mechanism for gold nanoparticle growth by reducing gold ions with hydrogen peroxide is applied in a plasmonic aptasensor, achieving naked-eye detection of thrombin at the single-molecule level based on the specific interaction of aptamer-thrombin via an arrested rolling circle amplification to yield horseradish peroxidase (hrp)-mimicking dnazymes as biocatalysts. | 2015 | 25864665 |
| magnetic-nanobead-based competitive enzyme-linked aptamer assay for the analysis of oxytetracycline in food. | this study presents a novel analytical method for the detection of oxytetracycline (otc) in complex food matrices based on a direct competitive enzyme-linked aptamer assay and magnetic separation technology. in this protocol, free otc competed with horseradish peroxidase labeled otc (otc-hrp) for binding to the otc aptamer immobilized on magnetic beads. the parameters that can affect the response, such as avidin concentration, aptamer concentration, otc-hrp concentration, incubation temperature, ... | 2015 | 25855149 |
| reagent pencils: a new technique for solvent-free deposition of reagents onto paper-based microfluidic devices. | custom-made pencils containing reagents dispersed in a solid matrix were developed to enable rapid and solvent-free deposition of reagents onto membrane-based fluidic devices. the technique is as simple as drawing with the reagent pencils on a device. when aqueous samples are added to the device, the reagents dissolve from the pencil matrix and become available to react with analytes in the sample. colorimetric glucose assays conducted on devices prepared using reagent pencils had comparable acc ... | 2015 | 25851055 |
| magnetosomes extracted from magnetospirillum magneticum strain amb-1 showed enhanced peroxidase-like activity under visible-light irradiation. | magnetosomes are intracellular structures produced by magnetotactic bacteria and are magnetic nanoparticles surrounded by a lipid bilayer membrane. magnetosomes reportedly possess intrinsic enzyme mimetic activity similar to that found in horseradish peroxidase (hrp) and can scavenge reactive oxygen species depending on peroxidase activity. our previous study has demonstrated the phototaxis characteristics of magnetospirillum magneticum strain amb-1 cells, but the mechanism is not well understoo ... | 2015 | 25837510 |
| continuous nanoflow-scanning electrochemical microscopy: voltammetric characterization and application for accurate and reproducible imaging of enzyme-labeled protein microarrays. | the coupling of scanning electrochemical microscopy (secm) to a continuous nanoflow (cnf) system is accomplished with the use of a microconcentric ring electrode/injector probe. the gold microring electrode encapsulated by a glass sheath is robust and can be beveled and polished. the cnf system, comprising a precision gas displacement pump and a rotary valve, is capable of delivering solution to the center of the secm probe in the range of 1-150 nl/min. major advantages of the cnf-secm imaging m ... | 2015 | 25831146 |
| improvements in neuroelectrophysiological and rear limb functions in rats with spinal cord injury after schwann cell transplantation in combination with a c5a receptor antagonist. | we measured the effect of schwann cell transplantation and complement factor 5a (c5a) receptor antagonist on nerve function recovery in rats with spinal cord injury. experimental spinal cord injury was induced in eighty wistar rats and these were randomly divided into four treatment groups: culture medium and saline injection (control group), schwann cell injection (cell transplantation group), c5a receptor antagonist injection (c5a receptor antagonist group), and both schwann cell and c5a recep ... | 2015 | 26634479 |
| utilization of enzyme-immobilized mesoporous silica nanocontainers (ibn-4) in prodrug-activated cancer theranostics. | to develop a carrier for use in enzyme prodrug therapy, horseradish peroxidase (hrp) was immobilized onto mesoporous silica nanoparticles (ibn-4: institute of bioengineering and nanotechnology), where the nanoparticle surfaces were functionalized with 3-aminopropyltrimethoxysilane and further conjugated with glutaraldehyde. consequently, the enzymes could be stabilized in nanochannels through the formation of covalent imine bonds. this strategy was used to protect hrp from immune exclusion, degr ... | 2015 | 28347114 |
| transplantation of erythropoietin gene-modified neural stem cells improves the repair of injured spinal cord. | the protective effects of erythropoietin on spinal cord injury have not been well described. here, the eukaryotic expression plasmid pcdna3.1 human erythropoietin was transfected into rat neural stem cells cultured in vitro. a rat model of spinal cord injury was established using a free falling object. in the human erythropoietin-neural stem cells group, transfected neural stem cells were injected into the rat subarachnoid cavity, while the neural stem cells group was injected with non-transfect ... | 2015 | 26604911 |
| self-propelled enzyme-based motors for smart mobile electrochemical and optical biosensing. | a millimeter-sized tubular motor for mobile biosensing of h2o2 in environmental and relevant clinical samples is reported. the concept relies on the self-propelled motion by the marangoni effect, where the asymmetric release of sds surfactant induces fluid convection and rapid dispersion of horseradish peroxidase (hrp) enzyme into the sample solution. this efficient movement together with the continuous release of fresh enzyme leads to greatly accelerated enzymatic reaction processes without the ... | 2015 | 26595193 |
| functionalized nanofiber meshes enhance immunosorbent assays. | three-dimensional substrates with high surface-to-volume ratios and subsequently large protein binding capacities are of interest for advanced immunosorbent assays utilizing integrated microfluidics and nanosensing elements. a library of bioactive and antifouling electrospun nanofiber substrates, which are composed of high-molecular-weight poly(oxanorbornene) derivatives, is described. specifically, a set of copolymers are synthesized from three 7-oxanorbornene monomers to create a set of water ... | 2015 | 26551162 |
| horseradish peroxidase and aptamer dual-functionalized nanoprobe for the amplification detection of alpha-methylacyl-coa racemase. | alpha-methylacyl-coa racemase (amacr) is over-expressed in many cancer types and can serve as a novel diagnostic biomarker. development of convenient and sensitive detection methods of amacr is of particular importance for cancer diagnosis. aptamers are a type of recognition elements, which possess many advantages over antibody, making them suitable for applications in biosensing and biotechnology. in this work, we use the efficient surface modification of gold nanoparticles (aunps) to prepare t ... | 2015 | 26547498 |
| sensitive electrochemical immunoassay for chlorpyrifos by using flake-like fe3o4 modified carbon nanotubes as the enhanced multienzyme label. | a highly sensitive electrochemical immunoassay of chlorpyrifos (cpf) was developed by using a biocompatible quinone-rich polydopamine nanospheres modified glass carbon electrode as the sensor platform and multi-horseradish peroxidase-flake like fe3o4 coated carbon nanotube nanocomposites as the signal label. due to the quinone-rich polydopamine nanospheres, the platform exhibited excellent fixing capacity by simple coating of sticky polydopamine nanospheres and subsequent oxidization. by copreci ... | 2015 | 26547497 |
| detection of hydrogen peroxide in photosystem ii (psii) using catalytic amperometric biosensor. | hydrogen peroxide (h2o2) is known to be generated in photosystem ii (psii) via enzymatic and non-enzymatic pathways. detection of h2o2 by different spectroscopic techniques has been explored, however its sensitive detection has always been a challenge in photosynthetic research. during the recent past, fluorescence probes such as amplex red (ar) has been used but is known to either lack specificity or limitation with respect to the minimum detection limit of h2o2. we have employed an electrochem ... | 2015 | 26528319 |
| shenqi fuzheng injection attenuates irradiation-induced brain injury in mice via inhibition of the nf-κb signaling pathway and microglial activation. | radiation-induced brain injury (ribi) is the most common and severe adverse effect induced by cranial radiation therapy (crt). in the present study, we examined the effects of the traditional chinese medicine shenqi fuzheng injection (sfi) on ribi in mice, and explored the underlying mechanisms. | 2015 | 26526200 |
| nanoprobe-initiated enzymatic polymerization for highly sensitive electrochemical dna detection. | electrochemical dna (e-dna) sensors have been greatly developed and play an important role in early diagnosis of different diseases. to determine the extremely low abundance of dna biomarkers in clinical samples, scientists are making unremitting efforts toward achieving highly sensitive and selective e-dna sensors. here, a novel e-dna sensor was developed taking advantage of the signal amplification efficiency of nanoprobe-initiated enzymatic polymerization (niep). in the niep based e-dna senso ... | 2015 | 26524941 |
| [goldmag particle-based chemiluminescence immunoassay for human high sensitive c-reactive protein]. | to develop a sensitive, accurate detection method for high sensitive c-reactive protein (hscrp). | 2015 | 26522353 |
| quantification of sorafenib in human serum by competitive enzyme-linked immunosorbent assay. | the multikinase inhibitor sorafenib has been used in the treatment of hepatocellular carcinoma, renal cell carcinoma, and differentiated thyroid carcinoma. here we have demonstrated the production of the first specific antibody against sorafenib. anti-sorafenib serum was obtained by immunizing mice with an antigen conjugated with bovine serum albumin and carboxylic modified 4-(4-aminophenoxy)-n-methyl-2-pyridinecarboxamide (ampc) using the n-succinimidyl ester method. enzyme labeling of sorafeni ... | 2015 | 26521829 |
| perineural capsaicin induces the uptake and transganglionic transport of choleratoxin b subunit by nociceptive c-fiber primary afferent neurons. | the distribution of spinal primary afferent terminals labeled transganglionically with the choleratoxin b subunit (ctb) or its conjugates changes profoundly after perineural treatment with capsaicin. injection of ctb conjugated with horseradish peroxidase (hrp) into an intact nerve labels somatotopically related areas in the ipsilateral dorsal horn with the exceptions of the marginal zone and the substantia gelatinosa, whereas injection of this tracer into a capsaicin-pretreated nerve also resul ... | 2015 | 26520849 |
| development of a c3c-based elisa method for the determination of anti-complementary potency of bupleurum polysaccharides. | traditionally, determination of inhibitory potency of complement inhibitors is performed by the hemolytic assay. however, this assay is not applicable to the lectin pathway, thus impeding the understanding of complement inhibitors against the overall function of the complement system. the main objective of our study was to develop a specific enzyme-linked immunosorbent assay (elisa) as an alternative method to assess the anti-complement activity, particularly against the lectin pathway. by using ... | 2015 | 26579461 |
| modeling of enhanced catalysis in multienzyme nanostructures: effect of molecular scaffolds, spatial organization, and concentration. | colocalized multistep enzymatic reaction pathways within biological catabolic and metabolic processes occur with high yield and specificity. spatial organization on membranes or surfaces may be associated with increased efficiency of intermediate substrate transfer. using a new brownian dynamics package, geombd, we explored the geometric features of a surface-anchored enzyme system by parallel coarse-grained brownian dynamics simulations of substrate diffusion over microsecond (μs) to millisecon ... | 2015 | 26574226 |
| pioneer neurons of the antennal nervous system project to protocerebral pioneers in the grasshopper schistocerca gregaria. | the twin nerve tracts of the antenna of the grasshopper schistocerca gregaria are established early in embryogenesis by sibling pairs of pioneers which delaminate from the epithelium into the lumen at the antennal tip. these cells can be uniquely identified via their co-expression of the neuronal labels horseradish peroxidase and the lipocalin lazarillo. the apical pioneers direct axons toward the antennal base where they encounter guidepost-like cells called base pioneers which transiently expr ... | 2015 | 26553379 |
| interrogating the activities of conformational deformed enzyme by single-molecule fluorescence-magnetic tweezers microscopy. | characterizing the impact of fluctuating enzyme conformation on enzymatic activity is critical in understanding the structure-function relationship and enzymatic reaction dynamics. different from studying enzyme conformations under a denaturing condition, it is highly informative to manipulate the conformation of an enzyme under an enzymatic reaction condition while monitoring the real-time enzymatic activity changes simultaneously. by perturbing conformation of horseradish peroxidase (hrp) mole ... | 2015 | 26512103 |
| general approach to the immobilization of glycoenzyme chains inside calcium alginate beads for bioassay. | a general method to obtain the efficient entrapment of mixtures of glycoenzymes in calcium alginate hydrogel is proposed in this paper. as a proof of principle, three glycoenzymes acting in series (trehalase, glucose oxidase, and horseradish peroxidase) have been coimmobilized in calcium alginate beads. the release of the enzymes from the hydrogel mesh (leakage) is avoided by exploiting the enzyme's aggregation induced by the concanavalin a. the aggregation process has been monitored by dynamic ... | 2015 | 26489723 |
| bio-based wrinkled surfaces harnessed from biological design principles of wood and peroxidase activity. | a new and simple approach for surface wrinkling inspired by polymer assemblies in wood fibers is introduced. a hard skin is synthesized on a linear polysaccharide support that resembles the structural units of the cell wall. this skin, a wood mimetic layer, is produced through immersion in a solution containing phenolic precursor and subsequent surface reaction by horseradish peroxidase. a patterned surface with micron-scale wrinkles is formed upon drying and as a result of inhomogeneous shrinka ... | 2015 | 26489384 |
| click synthesis of hydrophilic maltose-functionalized iron oxide magnetic nanoparticles based on dopamine anchors for highly selective enrichment of glycopeptides. | the development of methods to isolate and enrich low-abundance glycopeptides from biological samples is crucial to glycoproteomics. herein, we present an easy and one-step surface modification strategy to prepare hydrophilic maltose functionalized fe3o4 nanoparticles (nps). first, based on the chelation of the catechol ligand with iron atoms, azido-terminated dopamine (da) derivative was assembled on the surface of magnetic fe3o4 nanoparticles by sonication. second, the hydrophilic maltose-funct ... | 2015 | 26479949 |
| tailor-made boronic acid functionalized magnetic nanoparticles with a tunable polymer shell-assisted for the selective enrichment of glycoproteins/glycopeptides. | biomedical sciences, and in particular biomarker research, demand efficient glycoproteins enrichment platforms. in this work, we present a facile and time-saving method to synthesize phenylboronic acid and copolymer multifunctionalized magnetic nanoparticles (nps) using a distillation-precipitation polymerization (dpp) technique. the polymer shell is obtained through copolymerization of two monomers-affinity ligand 3-acrylaminophenylboronic acid (aapba) and a hydrophilic functional monomer. the ... | 2015 | 26479332 |
| thionine-modified poly(glycidyl methacrylate) nanospheres as labels of antibodies for biosensing applications. | monodisperse poly(glycidyl methacrylate) (pgma) nanospheres were obtained by emulsifier-free emulsion polymerization and characterized by physicochemical methods. the effects of various reaction parameters on the particle properties were investigated. the particle size was controlled in the range of 350-420 nm. to introduce carboxyl groups, the pgma nanospheres were hydrolyzed and oxidized with kmno4. subsequently, the enzyme horseradish peroxidase (hrp) and the electron mediator thionine were c ... | 2015 | 26479119 |
| optimized enzymatic colorimetric assay for determination of hydrogen peroxide (h2o2) scavenging activity of plant extracts. | the classical method to determine hydrogen peroxide (h2o2) scavenging activity of plant extracts is evaluated by measuring the disappearance of h2o2 at a wavelength of 230 nm. since this method suffers from the interference of phenolics having strong absorption in the uv region, a simple and rapid colorimetric assay was developed where plant extracts are introduced to h2o2, phenol and 4-aminoantipyrine reaction system in the presence of horseradish peroxidase (hrp). this reaction yields a quinon ... | 2015 | 26285798 |
| peroxidase induced oligo-tyrosine cross-links during polymerization of α-lactalbumin. | horseradish peroxidase (hrp) induced cross-linking of proteins has been reported to proceed through formation of di-tyrosine cross-links. in the case of low molar mass phenolic substrates, the enzymatic oxidation is reported to lead to polymerization of the phenols. the aim of this work was to investigate if during oxidative cross-linking of proteins oligo-tyrosine cross-links are formed in addition to dityrosine. to this end, α-lactalbumin (α-la) was cross-linked using horseradish peroxidase (h ... | 2015 | 26282909 |
| the molecular mechanism of the catalase-like activity in horseradish peroxidase. | horseradish peroxidase (hrp) is one of the most relevant peroxidase enzymes, used extensively in immunochemistry and biocatalysis applications. unlike the closely related catalase enzymes, it exhibits a low activity to disproportionate hydrogen peroxide (h2o2). the origin of this disparity remains unknown due to the lack of atomistic information on the catalase-like reaction in hrp. using qm(dft)/mm metadynamics simulations, we uncover the mechanism for reduction of the hrp compound i intermedia ... | 2015 | 26274391 |
| structure-activity relationship study on isothiocyanates: comparison of trpa1-activating ability between allyl isothiocyanate and specific flavor components of wasabi, horseradish, and white mustard. | allyl isothiocyanate (itc) (4) is the main pungent component in wasabi, and it generates an acrid sensation by activating trpa1. the flavor and pungency of itcs vary depending on the compound. however, the differences in activity to activate trpa1 between itcs are not known except for a few compounds. to investigate the effect of carbon chain length and substituents of itcs, the trpa1-activiting ability of 16 itcs was measured. since most of the itcs showed nearly equal trpa1-activiting potency, ... | 2015 | 26263397 |
| s1 nerve is the most efficient nerve rootlet innervating the anal canal and rectum in rats. | autonomic and somatic components participate in the defecation process in mammals, combining signals from the brainstem and forebrain. the innervation pattern involved in micturition in rats has been well studied, while defecation has been less studied. the aim of the present study was to identify the most important sensory and motor nerves of the anal canal and rectum involved in defecation. the amplitudes of evoked potential of the anal canal and rectum were higher when l6 and s1 ventral rootl ... | 2015 | 26260583 |
| hyaluronidase-incorporated hyaluronic acid-tyramine hydrogels for the sustained release of trastuzumab. | we developed an injectable hydrogel system for the sustained release of protein drugs that incorporated both protein drugs and hyaluronidase. trastuzumab and hyaluronidase were incorporated in hydrogels composed of hyaluronic acid-tyramine (ha-tyr) conjugates through the enzymatic crosslinking utilizing hydrogen peroxide (h2o2) and horseradish peroxidase (hrp). through electrostatic interactions with the ha, trastuzumab was retained in the hydrogel to minimize its burst release. hyaluronidase wa ... | 2015 | 26260452 |
| label-free real-time quantification of enzyme levels by interferometric spectroscopy combined with gelatin-modified nanoporous anodic alumina photonic films. | herein, we present an interferometric sensor based on the combination of chemically functionalized nanoporous anodic alumina photonic films (naa-pfs) and reflectometric interference spectroscopy (rifs) aimed to detect trace levels of enzymes by selective digestion of gelatin. the fabrication and sensing performance of the proposed sensor were characterized in real-time by estimating the changes in effective optical thickness (i.e., sensing principle) of gelatin-modified naa-pfs (i.e., sensing el ... | 2015 | 26259031 |
| a new label-free strategy for a highly efficient chemiluminescence immunoassay. | a new label-free chemiluminescence (cl) immunoassay method is proposed for the cheap, convenient and sensitive detection of proteins through the co-immobilization of a capture antibody and horseradish peroxidase on a sensing interface. the specific immunoreaction on the interface effectively inhibits the enzymatic cl reaction, thus causing a decrease in cl signals. | 2015 | 26248771 |
| carboxylic-group-functionalized single-walled carbon nanohorns as peroxidase mimetics and their application to glucose detection. | carboxylic-group-functionalized single-walled carbon nanohorns (swcnhs-cooh) have been found to possess peroxidase-like activity for the first time. similar to natural peroxidase, swcnhs-cooh can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine by h2o2 to produce a blue color solution. compared with horseradish peroxidase, swcnhs-cooh exhibit higher activity and stability under harsh reaction conditions. the catalytic activity of swcnhs-cooh depends on the concentration of h2o2. a colori ... | 2015 | 26247806 |
| [laminar location of neurons providing interhemispheric connections in the visual cortex in cats with impairments of binocular vision]. | the distributio of cells in the visual cortical layers of intact cats (n=7) and cats with experimentally induced strabismus (n=10) and monocular deprivation (n=5) was studied after microiontophoretic injection of horseradish peroxidase into the ocular-dominance columns in areas 17, 18 and the transition zone 17/18. it was found that in cats with impaired binocular vision, the callosal cells were located deeper in layers of i/ii, and higher - in layer iv, as compared to those in intact cats. also ... | 2015 | 26234033 |
| branched zinc oxide nanorods arrays modified paper electrode for electrochemical immunosensing by combining biocatalytic precipitation reaction and competitive immunoassay mode. | branched zinc oxide nanorods (bzr) arrays, an array with high charge carries collection efficiency and specific surface area, are grown on the reduced graphene oxide-paper working electrode for the first time to construct a paper-based electrochemical (ec) immunosensor. typically, the bzr are fabricated via a simple hydrothermal process, which can provide abundant sites for antibodies loading. by combining the large surface area of porous zinc oxide (pzs) and good biocompatibility of gold nanopa ... | 2015 | 26232677 |
| a repeatable assembling and disassembling electrochemical aptamer cytosensor for ultrasensitive and highly selective detection of human liver cancer cells. | in this work, a repeatable assembling and disassembling electrochemical aptamer cytosensor was proposed for the sensitive detection of human liver hepatocellular carcinoma cells (hepg2) based on a dual recognition and signal amplification strategy. a high-affinity thiolated tls11a aptamer, covalently attached to a gold electrode through au-thiol interactions, was adopted to recognize and capture the target hepg2 cells. meanwhile, the g-quadruplex/hemin/aptamer and horseradish peroxidase (hrp) mo ... | 2015 | 26231902 |
| mechanistic analysis of ultrasound assisted enzymatic desulfurization of liquid fuels using horseradish peroxidase. | this study has attempted to gain physical insight into ultrasound-assisted enzymatic desulfurization using system comprising horseradish peroxidase enzyme and dibenzothiophene (dbt). desulfurization pathway (comprising dbt-sulfoxide and dbt-sulfone as intermediates and 4-methoxy benzoic acid as final product) has been established with gc-ms analysis. intrinsic fluorescence and circular dichroism spectra of ultrasound-treated enzyme reveal conformational changes in secondary structure (reduction ... | 2015 | 26231128 |
| highly chemiluminescent graphene oxide hybrids bifunctionalized by n-(aminobutyl)-n-(ethylisoluminol)/horseradish peroxidase and sensitive sensing of hydrogen peroxide. | n-aminobutyl-n-ethylisoluminol and horseradish peroxidase bifunctionalized graphene oxide hybrids (abei-go@hrp) were prepared through a facile and green strategy for the first time. the hybrids exhibited excellent chemiluminescence (cl) activity over a wide range of ph from 6.1 to 13.0 when reacted with h2o2, whereas abei functionalized go had no cl emission at neutral ph and showed more than 2 orders of magnitude lower cl intensity than abei-go@hrp at ph 13.0. such strong cl emission from abei- ... | 2015 | 26230962 |
| bioinorganic nanocomposite hydrogels formed by hrp-gox-cascade-catalyzed polymerization and exfoliation of the layered composites. | the mild preparation of multifunctional nanocomposite hydrogels is of great importance for practical applications. we report that bioinorganic nanocomposite hydrogels, with calcium niobate nanosheets as cross-linkers, can be prepared by dual-enzyme-triggered polymerization and exfoliation of the layered composite. the layered hrp/calcium niobate composites (hrp=horseradish peroxidase) are formed by the assembly of the calcium niobate nanosheets with hrp. the dual-enzyme-triggered polymerization ... | 2015 | 26230284 |
| optimizing immobilization of avidin on surface-modified magnetic nanoparticles: characterization and application of protein-immobilized nanoparticles. | a simple optimization method of immobilization of avidin on magnetic nanoparticles (mnps)' surface was proposed in this study. the avidin-immobilized mnps were then developed and used to immobilize a model enzyme [horseradish peroxidase (hrp)]. the loading capacity (lc) and activity of avidin-immobilized mnps were optimized through selecting the most appropriate nanoparticle's size and shape, glutaraldehyde concentration, cross-linking reaction time, ultrasonic processing time, and initial conce ... | 2015 | 26224655 |
| microfabrication of photo-cross-linked hyaluronan hydrogels by single- and two-photon tyramine oxidation. | photo-cross-linking of tyramine-substituted hyaluronan (ha-tyr) hydrogels is demonstrated for the first time. ha-tyr hydrogels are fabricated via a rapid photosensitized process using visible light illumination. nontoxic conditions offer photoencapsulation of human mesenchymal stromal cells (hmscs) with high viability. macroscopic gels can be formed in less than 10 s, and one- and two-photon photopatterning enable 2d and 3d microfabrication. different degrees of cross-linking induce different sw ... | 2015 | 26222128 |
| expression and clinical implication of cyclooxygenase-2 and e-cadherin in oral squamous cell carcinomas. | epithelial-mesenchymal transition (emt) and angiogenesis are crucial events for development of aggressive and often fatal oral squamous cell carcinomas (osccs). both promote cancer progression and metastasis development, but while the former induces the loss of e-cadherin expression and, hence cadherin switching; the latter produces haematic blood vessel neo-formation and contribute to oscc cell growth, tumor mass development, and dissemination. cyclooxygenase-2 (cox-2) has an important role, no ... | 2015 | 26218314 |
| rapid and sensitive identification of marine bacteria by an improved in situ dna hybridization chain reaction (quickhcr-fish). | catalyzed reporter deposition-fluorescence in situ hybridization (card-fish) with rrna-targeted oligonucleotide probes has significantly improved the identification of microorganisms in various environmental samples. however, one of the major constraints of card-fish is the low probe penetration due to the high molecular weight of the horseradish peroxidase (hrp) label. recently, this limitation has been overcome by a novel signal amplification approach termed in situ dna-hybridization chain rea ... | 2015 | 26215142 |
| multienzyme inkjet printed 2d arrays. | the use of printing to produce 2d arrays is well established, and should be relatively facile to adapt for the purpose of printing biomaterials; however, very few studies have been published using enzyme solutions as inks. among the printing technologies, inkjet printing is highly suitable for printing biomaterials and specifically enzymes, as it offers many advantages. formulation of the inkjet inks is relatively simple and can be adjusted to a variety of biomaterials, while providing nonharmfu ... | 2015 | 26214072 |
| a afferent fibers are involved in the pathology of central changes in the spinal dorsal horn associated with myofascial trigger spots in rats. | a afferent fibers have been reported to participate in the development of the central sensitization induced by inflammation and injuries. current evidence suggests that myofascial trigger points (mtrps) induce central sensitization in the related spinal dorsal horn, and clinical studies indicate that a fibers are associated with pain behavior. because most of these clinical studies applied behavioral indexes, objective evidence is needed. additionally, mtrp-related neurons in dorsal root ganglia ... | 2015 | 26210690 |
| dual-signal amplified electrochemiluminescence immunoassay for salbutamol based on quantum dots and gold nanoparticle-labeled horseradish peroxidase. | this study describes a novel electrochemical immunosensor to amplify the electrochemiluminescence (ecl) signal for the ultrasensitive detection of salbutamol (sal) using quantum dots (qds) and gold nanoparticle (aunp) conjugated horseradish peroxidase (hrp). the electrochemical detection was based on the hrp catalyzed consumption of self-produced h2o2, which has been extensively used as a co-reactant of qds, by o-phenylenediamine (opd). the enzymatic reaction rate is proportional to the amount o ... | 2015 | 26200776 |
| designing hydrophobically modified polysaccharide derivatives for highly efficient enzyme immobilization. | in this contribution, a hydrophobically modified polysaccharide derivative is synthesized in an eco-friendly solvent water by conjugation of benzylamine with the backbone of the biopolymer. owing to the presence of aromatic moieties, the resulting water-soluble polysaccharide derivative self-assembles spontaneously and selectively from solution on the surface of nanometric thin films and sheets of polystyrene (ps). the synthetic polymer modified in this way bears a biocompatible nanolayer suitab ... | 2015 | 26200740 |
| protein microarray with horseradish peroxidase chemiluminescence for quantification of serum α-fetoprotein. | to develop and evaluate a protein microarray assay with horseradish peroxidase (hrp) chemiluminescence for quantification of α-fetoprotein (afp) in serum from patients with hepatocellular carcinoma (hcc). | 2015 | 26198141 |
| glucose biosensor based on gox/hrp bienzyme at liquid-crystal/aqueous interface. | glucose oxidase (gox) and horseradish peroxidase (hrp) were co-immobilized to the polyacrylicacid block of a poly(acrylicacid-b-4-cyanobiphenyl-4'-undecylacrylate) (paa-b-lcp) copolymer in water. paa-b-lcp was strongly anchored by the lcp block in 4-cyano-4'-pentylbiphenyl (5cb) which was contained in a transmission electron microscope (tem) grid for glucose detection. the optimal conditions for the performance of the tem grid glucose biosensor were studied in terms of the activity and stability ... | 2015 | 26196711 |
| development of a carbon dot (c-dot)-linked immunosorbent assay for the detection of human α-fetoprotein. | a sensitive, selective, environmentally friendly, high-throughput, well-plate-based immunosorbent assay was developed to detect human α-fetoprotein (afp) using carbon dots (c-dots). highly fluorescent c-dots were synthesized using a one-step hydrothermal reaction, with citric acid serving as the carbon source and ethylene diamine acting as the nitrogen source. the reaction conditions were optimized to obtain the desired surface functionality. then, the c-dots were used to label one member of the ... | 2015 | 26186018 |
| enzymatic hydrolysate-induced displacement reaction with multifunctional silica beads doped with horseradish peroxidase-thionine conjugate for ultrasensitive electrochemical immunoassay. | a novel (invertase) enzymatic hydrolysate-triggered displacement reaction strategy with multifunctional silica beads, doped with horseradish peroxidase-thionine (hrp-thi) conjugate, was developed for competitive-type electrochemical immunoassay of small molecular aflatoxin b1 (afb1). the competitive-type displacement reaction was carried out on the basis of the affinity difference between enzymatic hydrolysate (glucose) and its analogue (dextran) for concanavalin a (con a) binding sites. initial ... | 2015 | 26181887 |
| carbon nanotubes labeled with aptamer and horseradish peroxidase as a probe for highly sensitive protein biosensing by postelectropolymerization of insoluble precipitates on electrodes. | carbon nanotubes (cnts) labeled with aptamer and horseradish peroxidase (hrp) were used as a probe to amplify the impedimetric sensing of the aptamer-protein (with thrombin as the model) interaction. the hrp-biocatalyzed oxidation of 3,3-diaminobenzidine (dab) in the presence of h2o2 and the postelectropolymerization of insoluble precipitates produced on the electrode supports were used as a signal amplification route for the sensing process. thrombin was sensed by aptamer 1 immobilized on a gla ... | 2015 | 26176595 |
| artificial peroxidase/oxidase multiple enzyme system based on supramolecular hydrogel and its application as a biocatalyst for cascade reactions. | inspired by delicate structures and multiple functions of natural multiple enzyme architectures such as peroxisomes, we constructed an artificial multiple enzyme system by coencapsulation of glucose oxidases (gox) and artificial peroxidases in a supramolecular hydrogel. the artificial peroxidase was a functional complex micelle, which was prepared by the self-assembly of diblock copolymer and hemin. compared with catalase or horseradish peroxidase (hrp), the functional micelle exhibited comparab ... | 2015 | 26173996 |
| propofol injection combined with bone marrow mesenchymal stem cell transplantation better improves electrophysiological function in the hindlimb of rats with spinal cord injury than monotherapy. | the repair effects of bone marrow mesenchymal stem cell transplantation on nervous system damage are not satisfactory. propofol has been shown to protect against spinal cord injury. therefore, this study sought to explore the therapeutic effects of their combination on spinal cord injury. rat models of spinal cord injury were established using the weight drop method. rats were subjected to bone marrow mesenchymal stem cell transplantation via tail vein injection and/or propofol injection via tai ... | 2015 | 26170827 |
| restriction site free cloning (rsfc) plasmid family for seamless, sequence independent cloning in pichia pastoris. | tagging proteins is a standard method facilitating protein detection, purification or targeting. when tagging a certain protein of interest, it is challenging to predict which tag will give optimal results and will not interfere with protein folding, activity or yields. ideally, multiple tags and positions are tested which however complicates molecular cloning and expression vector generation. in conventional cloning, tags are either added on pcr primers (requiring a distinct primer and pcr prod ... | 2015 | 26169367 |
| highly sensitive chemiluminescent detection of lead ion based on its displacement of potassium in g-quadruplex dnazyme. | a simple and highly sensitive method for detecting lead ion (pb(2+)) in biosamples was developed based on its displacement of potassium in g-quadruplex dnazyme, which can catalyze the luminol-h2o2 chemiluminescence (cl) reaction. by introducing a g-rich dna sequence, ps2.m, which can fold into a g-quadruplex when binding with hemin in the presence of k(+) and act as a superior horseradish peroxidase (hrp) mimicking-enzyme, we found this dnazyme can effectively catalyze the h2o2-mediated oxidatio ... | 2015 | 26165488 |
| an extended-gate type organic fet based biosensor for detecting biogenic amines in aqueous solution. | this study is the first to report on the detection of biogenic amines in an aqueous solution using an organic field-effect transistor (ofet) device with an extended gate electrode modified with a layer of diamine oxidase and a horseradish peroxidase osmium-redox polymer. the limit of detection (lod) for histamine was estimated to be 1.2 μm. these results reveal that extended-gate type ofet devices are highly suitable enzyme-based biosensors for the detection of biogenic amine levels. | 2015 | 26165298 |
| electrochemical aptasensor for the detection of adenosine by using pdcu@mwcnts-supported bienzymes as labels. | a highly sensitive electrochemical adenosine aptasensor was fabricated by covalently immobilizing 3'-nh2-terminated capture probe (ssdna1) and thionine (th) on au-gs modified glassy carbon electrode. 3'-sh-terminated adenosine aptamer (ssdna2) was adsorbed onto palladium/copper alloyed supported on mwcnts (pdcu@mwcnts)-conjugated multiple bienzymes, glucose oxidase (gox), and horseradish peroxidase (hrp) (ssdna2/pdcu@mwcnts/hrp/gox). then, it was immobilized onto the electrode surface through th ... | 2015 | 26164010 |
| tyramide signal amplification for immunofluorescent enhancement. | enzyme-linked signal amplification is a key technique used to enhance the immunohistochemical detection of protein, mrna, and other molecular species. tyramide signal amplification (tsa) is based on a catalytic reporter deposit in close vicinity to the epitope of interest. the advantages of this technique are its simplicity, enhanced sensitivity, high specificity, and compatibility with modern multi-label fluorescent microscopy. here, we describe the use of a tsa kit to increase the signal of en ... | 2015 | 26160574 |
| direct elisa. | first described by engvall and perlmann, the enzyme-linked immunosorbent assay (elisa) is a rapid and sensitive method for detection and quantitation of an antigen using an enzyme-labeled antibody. besides routine laboratory usage, elisa has been utilized in medical field and food industry as diagnostic and quality control tools. traditionally performed in 96-well or 384-well polystyrene plates, the technology has expanded to other platforms with increase in automation. depending on the antigen ... | 2015 | 26160564 |
| bioconjugation of antibodies to horseradish peroxidase (hrp). | the bioconjugation of an antibody to an enzymatic reporter such as horseradish peroxidase (hrp) affords an effective mechanism by which immunoassay detection of a target antigen can be achieved. the use of heterobifunctional cross-linkers to covalently link antibodies to hrp provides a simple and convenient means to maintain antibody affinity while imparting a functional reporter used for antigen detection. in this chapter, we describe a process by which sulfo-smcc is used to generate a stable m ... | 2015 | 26160562 |
| clinical relevance of mir-mediated hla-g regulation and the associated immune cell infiltration in renal cell carcinoma. | in human tumors of distinct origin including renal cell carcinoma (rcc), the non-classical human leukocyte antigen g (hla-g) is frequently expressed, thereby inhibiting the cytotoxic activity of t and natural killer (nk) cells. recent studies demonstrated a strong post-transcriptional gene regulation of the hla-g by mir-152, -148a, -148b and -133a. standard methods were applied to characterize the expression and function of hla-g, hla-g-regulatory micrornas (mirs) and the immune cell infiltratio ... | 2015 | 26155421 |
| [reconstruction of lower extremity function of complete spinal cord injury rats by first neuron connection]. | to investigate the effects of the first neuron connection for the reconstruction of lower extremity function of complete spinal cord injury rats. | 2015 | 27044224 |
| ultraviolet-b protective effect of flavonoids from eugenia caryophylata on human dermal fibroblast cells. | the exposure of skin to ultraviolet-b (uv-b) radiations leads to deoxyribonucleic acid (dna) damage and can induce production of free radicals which imbalance the redox status of the cell and lead to increased oxidative stress. clove has been traditionally used for its analgesic, anti-inflammatory, anti-microbial, anti-viral, and antiseptic effects. | 2015 | 26929573 |
| changes in immunoexpression of p53, ki-67, ets-1, apaf-1 and pten in serrated and conventional colon adenomas. | the balance between apoptosis and proliferation is tipped towards a decrease of apoptosis as the colonocyte progresses in the adenoma to carcinoma sequence of colon carcinogenesis. according to literature data, proteins like p53, ki-67, apaf-1, ets-1, pten contribute to inhibition of apoptosis and stimulation of proliferation. | 2015 | 26743285 |
| chromatographic evaluation and application of silica based azide-modified stationary phase under hydrophilic interaction liquid chromatography mode. | hydrophilic interaction liquid chromatography (hilic) has attracted more and more interests in recent years. however, the stationary phases for hilic are still scarce. more stationary phases and separation methods should be developed. silica based azide-modified stationary phase was synthesized and characterized by our group. the nucleosides were selected as the test samples. the retention property of nucleosides on the azide-modified stationary phase was shown with typical hilic characterizatio ... | 2015 | 26753277 |
| the antitumor effect of tanshinone iia on anti-proliferation and decreasing vegf/vegfr2 expression on the human non-small cell lung cancer a549 cell line. | the effects of tanshinone iia on the proliferation of the human non-small cell lung cancer cell line a549 and its possible mechanism on the vegf/vegfr signal pathway were investigated. the exploration of the interaction between tanshinone iia and its target proteins provides a feasible platform for studying the anticancer mechanism of active components of herbs. the cck-8 assay was used to evaluate the proliferative activity of a549 cells treated with tanshinone iia (2.5-80 μmol/l) for 24, 48 an ... | 2015 | 26713270 |
| [treatment of transplantation of nt-3 gene modified olfactory ensheathing cells on experimental autoimmune encephalomyelitis in rats]. | to explore the effects of transplantation of nt-3 gene modified olfactory ensheathing cells (oecs) on nerve function of rats with experimental autoimmune encephalomyelitis (eae). | 2015 | 26710917 |
| injectable hydrogel systems crosslinked by horseradish peroxidase. | hydrogels are widely used as reservoirs in drug delivery and scaffolds for tissue engineering. in particular, injectable hydrogel systems, which are formed by physical, chemical, or enzyme-mediated crosslinking reactions in situ, offer the advantages of minimal invasiveness, ease of application, and void-filling property. examples of these hydrogels are provided in the first part of this paper. in the second part, hydrogels that are formed by the enzymatic activity of horseradish peroxidase (hrp ... | 2015 | 26694014 |
| studies on horseradish peroxidase. 13. the kinetic effect of cyanide on the oxidation-reduction cycle. | 2015 | 4706838 | |
| canine distemper virus infection among wildlife before and after the epidemic. | in 2007-2008, a canine distemper virus (cdv) epidemic occurred among wild animals in wakayama prefecture, japan, and many mammals, including the wild boar and deer, were infected. in this study, cdv prevalence among wild animals was surveyed before and after the epidemic. at first, an enzyme-linked immunosorbent assay (elisa) with horseradish peroxidase-conjugated protein a/g was established to detect cdv antibodies in many mammalian species. this established elisa was available for testing dogs ... | 2015 | 26074342 |
| peroxidase activity and involvement in the oxidative stress response of roseobacter denitrificans truncated hemoglobin. | roseobacter denitrificans is a member of the widespread marine roseobacter genus. we report the first characterization of a truncated hemoglobin from r. denitrificans (rd. trhb) that was purified in the heme-bound form from heterologous expression of the protein in escherichia coli. rd. trhb exhibits predominantly alpha-helical secondary structure and absorbs light at 412, 538 and 572 nm. the phylogenetic classification suggests that rd. trhb falls into group ii trhbs, whereas sequence alignment ... | 2015 | 25658318 |
| development and evaluation of an in-house igm-capture elisa for the detection of chikungunya and its application to a dengue outbreak situation in kenya in 2013. | chikungunya (chik) is a mosquito-borne viral disease. in the 2004 chik outbreak in kenya, diagnosis was delayed because of the lack of accurate diagnostics. therefore, this study aimed to develop and evaluate an in-house igm-capture enzyme linked immunosorbent assay (elisa) (in-house elisa) for the detection of chikungunya virus (chikv) infections. anti-chikv antibodies were raised in rabbits, purified and conjugated to horseradish peroxidase. these anti-chikv antibodies and cell-culture derived ... | 2015 | 25866119 |
| the fucomic potential of mosquitoes: fucosylated n-glycan epitopes and their cognate fucosyltransferases. | fucoconjugates are key mediators of protein-glycan interactions in prokaryotes and eukaryotes. as examples, n-glycans modified with the non-mammalian core α1,3-linked fucose have been detected in various organisms ranging from plants to insects and are immunogenic in mammals. the rabbit polyclonal antibody raised against plant horseradish peroxidase (anti-hrp) is able to recognize the α1,3-linked fucose epitope and is also known to specifically stain neural tissues in the fruit fly drosophila me ... | 2015 | 26617287 |
| combining protein and strain engineering for the production of glyco-engineered horseradish peroxidase c1a in pichia pastoris. | horseradish peroxidase (hrp), conjugated to antibodies and lectins, is widely used in medical diagnostics. since recombinant production of the enzyme is difficult, hrp isolated from plant is used for these applications. production in the yeast pichia pastoris (p. pastoris), the most promising recombinant production platform to date, causes hyperglycosylation of hrp, which in turn complicates conjugation to antibodies and lectins. in this study we combined protein and strain engineering to obtain ... | 2015 | 26404235 |
| multifunctional fe₃o₄ core/ni-al layered double hydroxides shell nanospheres as labels for ultrasensitive electrochemical immunoassay of subgroup j of avian leukosis virus. | a novel electrochemical immunosensor for ultrasensitive detection of subgroup j of avian leukosis virus (alvs-j) was designed by using graphene sheets (gs)-layered double hydroxides (ldhs) composites modified electrode with multifunctional fe(3)o(4) core/ni-al ldhs shell (ldhs@fe(3)o(4)) nanospheres as labels. at first, the gs-ldhs were used for the immunosensor platform for improving the electronic transmission rate as well as increasing the surface area to capture a large amount of primary ant ... | 2015 | 22621982 |
| [immunochemical detection of azospirilla in soil with genus-specific antibodies]. | immunoelectrophoresis and immunodiffusion analysis with antibodies to whole intact cells of the type strain of nitrogen-fixing soil bacteria azospirillum brasilense sp7 revealed at least three conservative surface immunogenic proteins of azospirilla. cross-reactions with these proteins made it possible to use the above antibodies for detection of azospirilla as a genus-specific probe conjugated with horseradish peroxidase as an enzymatic label. direct immune-enzyme analysis of soil suspensions ( ... | 2015 | 26263631 |
| strong protection against ricin challenge induced by a novel modified ricin a-chain protein in mouse model. | ricin toxin (rt) is an extremely potent toxin derived from the castor bean plant. as a possible bioterrorist weapon, it was categorized as a level b agent in international society. with the growing awareness and concerns of the "white powder incident" in recent years, it is indispensable to develop an effective countermeasure against rt intoxication. in this study we used site-directed mutagenesis and polymerase chain reaction (pcr) techniques to modify the gene of ricin a-chain (rta). as a resu ... | 2015 | 26038805 |
| ultra-high-throughput screening of an in vitro-synthesized horseradish peroxidase displayed on microbeads using cell sorter. | the c1a isoenzyme of horseradish peroxidase (hrp) is an industrially important heme-containing enzyme that utilizes hydrogen peroxide to oxidize a wide variety of inorganic and organic compounds for practical applications, including synthesis of fine chemicals, medical diagnostics, and bioremediation. to develop a ultra-high-throughput screening system for hrp, we successfully produced active hrp in an escherichia coli cell-free protein synthesis system, by adding disulfide bond isomerase dsbc a ... | 2015 | 25993095 |
| high-yield reactivation of anionic tobacco peroxidase overexpressed in escherichia coli. | anionic tobacco peroxidase (top) is extremely active in chemiluminescence reaction of luminol oxidation without addition of enhancers and more stable than horseradish peroxidase under antibody conjugation conditions. in addition, recombinant top (rtop) produced in escherichia coli is known to be a perfect direct electron transfer catalyst on electrodes of various origin. these features make the task of development of a high-yield reactivation protocol for rtop practically important. previous att ... | 2015 | 25986322 |
| site-directed mutagenesis of tobacco anionic peroxidase: effect of additional aromatic amino acids on stability and activity. | tobacco anionic peroxidase (top) is known to effectively catalyze luminol oxidation without enhancers, in contrast to horseradish peroxidase (hrp). to pursue structure-activity relationship studies for top, two amino acids have been chosen for mutation, namely thr151, close to the heme plane, and phe140 at the entrance to the active site pocket. three mutant forms top f140y, t151w and f140y/t151w have been expressed in escherichia coli, and reactivated to yield active enzymes. single-point mutat ... | 2015 | 25957835 |