Publications

anatomic relations of the thoracic pedicle to the adjacent neural structures.	this study analyzed anatomic parameters between the thoracic pedicles and the spinal nerve roots.	1997	9253087
medical therapy of graves' disease: does thyroxine prevent recurrence of hyperthyroidism?	sixty patients with graves' disease (gd) hyperthyroidism were distributed in two randomized groups. patients in group a (n = 30) received carbimazole by a titration regimen, and patients in group b (n = 30) were treated with higher doses of carbimazole plus t4. clinical and analytical evaluations were done at baseline, during treatment (18.4 +/- 2.6 months), and after, until the relapse of hyperthyroidism, or for 4.98 +/- 1.6 yr in patients who did not relapse. there were no differences in clini ...	1997	9253309
minimal cardiac effects in asymptomatic athyreotic patients chronically treated with thyrotropin-suppressive doses of l-thyroxine.	biondi, fazio, and colleagues recently reported that long term t4 treatment to suppress serum tsh markedly affects cardiac function. t4-treated patients had more symptoms [12.2 +/- 3.9 (+/-sd) vs. 4.2 +/- 2.3 by quantitative questionnaire], higher mean heart rate, increased incidence of atrial extrasystoles, increased interventricular septal thickness and left ventricular mass index (lvmi), and significant diastolic dysfunction. the severity of cardiac abnormalities was highly correlated with sc ...	1997	9253339
fasting and postprandial lipid abnormalities in hypopituitary women receiving conventional replacement therapy.	hypopituitary patients, particularly women, have excess mortality, mostly due to vascular disease. we have studied circulating lipid and lipoprotein concentrations, fasting and over 24 h, in hypopituitary women and men and in matched controls. firstly, 67 hypopituitary patients (36 women) and 87 normal controls (54 women) were studied after an overnight fast. secondly, 12 patients (6 women) and 14 matched controls (7 women) were studied over 24 h of normal meals and activity. the patients were a ...	1997	9253349
early assessment of transplanted liver function: lignocaine clearance test (megx).	the purpose of this study was to assess the value of lignocaine biotransformation into monoethylglycinexylidide (megx) and conventional liver function tests in the early post-operative period as an indicator of graft function and as a diagnostic tool for complications after hepatic transplantation. monoethylglycinexylidide formation, plasma bilirubin, aspartate aminotransferase (asat), alanine aminotransferase (alat), factor v index (fvi) and prothrombin time index (pti) were measured in 71 pati ...	1997	9253568
effects of base mismatches on joining of short oligodeoxynucleotides by dna ligases.	the requirement for watson-crick base pairing surrounding a nick in duplex dna to be sealed by dna ligase is the basis for oligonucleotide ligation assays that distinguish single base mutations in dna targets. experiments in a model system demonstrate that the minimum length of oligonucleotide that can be joined differs for different ligases. thermus thermophilus (tth) dna ligase is unable to join any oligonucleotide of length six or less, while t4 dna ligase and t7 dna ligase are both able to j ...	1997	9254695
precise assessment of microsatellite instability using high resolution fluorescent microsatellite analysis.	the instability of microsatellite sequences dispersed in the genome has been linked to a deficiency in cellular mismatch repair. this phenotype has been frequently observed in various human neoplasms and is regarded as a major factor in tumorigenesis. to demonstrate alterations in microsatellite sequences, polymerase chain reaction (pcr) and electrophoretic analysis are techniques often used. however, the electrophoretic profiles of pcr-amplified microsatellite sequences have not been well chara ...	1997	9254697
ph dependence of self-splicing by the group ia2 intron in a pre-mrna derived from the nrdb gene of bacteriophage t4.	the nrdb gene of bacteriophage t4 contains a group ia2 intron. we have investigated the kinetics of self-splicing by a shortened variant of nrdb pre-mrna in the presence of the co-substrates guanosine and 2'-amino-2'-deoxyguanosine. the ph dependence of the first transesterification step displayed parallel linear correlations for the two different co-substrates up to ph 7, above which the reaction with guanosine levels off to become ph independent. the plot for the 30-fold slower reaction with 2 ...	1997	9254717
postnatal development of the exocrine pancreas in suckling goat kids.	a total of 25 preruminant granadina breed goats were used. they were bottle-fed goat milk ad libitum from postnatal day 3 to 28. until the age of 3 d, kids were fed colostrum. body weight, pancreas weight, total protein concentration, enzyme activities in pancreatic tissue and hormone concentrations (cortisol, gastrin, t3 and t4) were determined at 3, 7, 14, 21 and 28 d of age. our results show that the rates of pancreatic synthesis and secretion of chymotrypsin are well developed at birth in th ...	1997	9255407
a multicenter trial evaluation of the fibrin/fibrinogen degradation products test for detection and monitoring of bladder cancer.	presently there is a lack of effective, noninvasive tests for the detection and monitoring of bladder cancer. measurement of fibrin/fibrinogen degradation products in urine has been shown to be a useful indicator of bladder carcinoma. the objective of this study was to evaluate the auratek fdp rapid immunoassay device for the detection of urinary fibrin/ fibrinogen degradation products associated with bladder cancer.	1997	9258086
characterization of an amino-terminal fragment of the bacteriophage t4 uvsy recombination protein.	the uvsy protein plays essential roles in homologous genetic recombination processes in the bacteriophage t4. in vitro, uvsy promotes the formation of presynaptic filaments containing stoichiometric amounts of the t4 uvsx recombinase bound to single-stranded dna. uvsy protein has intrinsic binding activities towards ssdna, uvsx, and gp32, the t4-encoded ssb, however, it has not been directly determined which of these activities are essential for uvsy's role in presynapsis. we have therefore soug ...	1997	9258436
influences of obesity and weight loss on thyroid hormones. a 3-3.5-year follow-up study on obese subjects with surgical bilio-pancreatic by-pass.	the effects of changing body size, energy intake and substrate oxidation on serum t4, ft4, t3, ft3 and tsh were investigated in ten morbidly obese subjects (4 men/6 women; age: 37 +/- 6 years; bmi: 53.8 +/- 6.5 kg/m2; mean +/- sd) who had undergone a surgical bilio-pancreatic by-pass in order to reduce their body weight. the starting value of serum ft3 was inversely related to the bmi (r = -0.63; p < 0.05). after 1-3 months, all the subjects were losing weight and their intake of carbohydrates w ...	1997	9258807
sodium ipodate (oragrafin) in the preoperative preparation of graves' hyperthyroidism.	fourteen graves' hyperthyroid patients who had been prepared for surgery with sodium ipodate (si) 500 mg orally twice daily for 3 days were retrospectively studied. si was administered in combination with propylthiouracil (10 cases) and beta blockers (all cases), which had been previously initiated. free serum thyroxine (t4) and total triiodothyronine (t3) concentrations were measured before and after si therapy on the morning of surgery. si treatment significantly reduced total t3 concentration ...	1997	9261010
structure of bacteriophage t4 fibritin: a segmented coiled coil and the role of the c-terminal domain.	oligomeric coiled-coil motifs are found in numerous protein structures; among them is fibritin, a structural protein of bacteriophage t4, which belongs to a class of chaperones that catalyze a specific phage-assembly process. fibritin promotes the assembly of the long tail fibers and their subsequent attachment to the tail baseplate; it is also a sensing device that controls the retraction of the long tail fibers in adverse environments and, thus, prevents infection. the structure of fibritin ha ...	1997	9261070
preliminary results of a phase ii study of high-dose radiation therapy and neoadjuvant plus concomitant 5-fluorouracil with cddp chemotherapy for patients with anal canal cancer: a french cooperative study.	chemotherapy (5-fluorouracil-mitomycin c) concomitant with radiotherapy (rt) increases local control and colostomy-free survival in advanced anal canal carcinomas (acc). the purpose of this prospective trial was to analyse the toxicity of and response to an induction chemotherapy combining 5-fluorouracil (5-fu) and cddp administered concomitantly with irradiation.	1997	9261527
the mechanisms of transient hypothyroxinemia in infants born to mothers with graves' disease.	transient hypothyroxinemia in infants born to mothers with graves' disease is a unique disorder first reported by us in 1988. most mothers of these infants have had no treatment, are diagnosed as having thyrotoxicosis during the last trimester, or were not well controlled during pregnancy. these infants are believed to have transient central hypothyroidism, the mechanisms of which have not been elucidated. we measured tsh-receptor antibody activities in maternal serum and blood thyroxine (t4) (f ...	1997	9262225
inhibition of tryptase tl2 from human t4+ lymphocytes and inhibition of hiv-1 replication in h9 cells by recombinant aprotinin and bikunin homologues.	the serine esterase tl2 from human t4+ lymphocytes is a binding component to hiv-1 glycoprotein gp120 and seems to play a role in the hiv-1 infection mechanism. recombinant variants of the kunitz-type serine proteinase inhibitor aprotinin were investigated for their ability to inhibit tryptase tl2 and the binding of gp120 to this enzyme. furthermore, the viral replication of hiv-1 was investigated h9 cell cultures under the influence of recombinant aprotinin and bikunin variants. in contrast to ...	1997	9263127
myelin basic protein immunoreactivity in the internal capsule of neonates from rats on a low iodine intake or on methylmercaptoimidazole (mmi).	rats fed on low iodine diets (lids) result in a normal circulating level of triiodothyronine (t3), a low level of thyroxine (t4) and an elevated thyroid-stimulating hormone (tsh). these changes are similar to those observed in habitants who live in iodine-deficient areas and different from those observed when the hypothyroidism is produced by goitrogens. to study the effects of lid or goitrogens on the myelin basic protein (mbp) immunoreactivity (mbp-ir) during the myelination of the internal ca ...	1997	9263597
design for an ipriflavone multicenter european fracture study.	in order to investigate the efficacy of ipriflavone (i.p.) on the prevention of vertebral fractures and the effect on bone mineral density (bmd) in women with postmenopausal osteoporosis, a large multicentric european study was designed and is presently ongoing. included in the study were 460 caucasian, nonobese postmenopausal women aged > 45 and < 75 years, menopaused for at least 12 months. inclusion was on the basis of a lumbar bone mineral density (bmd) lower than 2 sd compared with healthy ...	1997	9263614
residues at the carboxy terminus of t4 dna polymerase are important determinants for interaction with the polymerase accessory proteins.	three t4 dna polymerase accessory proteins (44p/62p and 45p) stimulate the polymerase (pol) activity and the 3'-5' exonuclease (exo) activity of t4 dna polymerase (43p) on long, double-stranded dna substrates. the 44p/62p "clamp loader" facilitates the binding of 45p, the "sliding clamp", to dna that is primed for replication. using a series of truncated 43p mutants, we identified a region at the extreme carboxy terminus of the dna polymerase that is required for its interaction with accessory p ...	1997	9265627
treatment of thyroid disease in pregnancy.	with appropriate therapy, complications related to thyroid disease in pregnancy can be minimized. although the diagnosis of thyroid endocrinopathy may be difficult in pregnancy, few therapies are contra-indicated. because medications may cross the placenta, however, clinicians need always to be mindful of potential fetal effects and should work to use the minimal dose necessary to achieve maternal euthyroidism. thyroid function tests, in particular free t4 and tsh, remain good measures of thyroi ...	1997	9266579
formation of novel c1-oxidised abasic sites in alkylperoxyl radical-damaged plasmid dna.	we have recently shown that peroxyl radicals react with dna to form alkali-labile sites. to further characterise these lesions, we studied their susceptibility to digestion by repair endonucleases that recognise different types of abasic sites. we found that peroxyl radical-damaged psp189 plasmids were resistant to cleavage by t4 endonuclease v, an enzyme that incises dna at "regular" and c4-oxidised abasic residues. in contrast, the dna was digested by exonuclease iii, an enzyme that recognises ...	1997	9266818
drug eruption induced by cyanamide (carbimide): a clinical and histopathologic study of 7 patients.	in some countries, cyanamide is used as an alcohol intake inhibitor with few harmful side effects. so far 3 cases of allergic contact dermatitis and only 1 of lichenoid drug eruption due to cyanamide have been reported.	1997	9267733
a rare neurological presentation due to ossification of the posterior longitudinal ligament of the thoracic spine and ankylosing spondylitis: case report.	ossification of the posterior longitudinal ligament (opll) may be associated with certain rheumatic conditions including ankylosing spondylitis (as), diffuse idiopathic skeletal hyperostosis (dish) or spondylosis. more than 95% of all opll are localized at the cervical spine. herein, we report a case of opll at the thoracic spine in an hla-b27-positive female patient with ankylosing spondylitis. the patient was presented to use with spastic paraparesis. the imaging studies included plain roentge ...	1997	9267923
triple-helical dna as a reversible block of the branch point in a partially symmetrical dna four-arm junction.	dna branch migration is a fundamental process in genetic recombination. a new model system has been developed for studying branch migration in a small synthetic four-arm junction. a mathematical method for describing branch-point movement by discrete steps in such junctions is also presented. the key to our experimental system is the ability to fix the location of the branch point during the assembly of the junction with a reversible block. the block is provided by a short oligonucleotide that f ...	1997	9268664
heat effects on the repair of dna double-strand breaks in cho cells.	the effect of heat (43-45 degrees c) on the induction and the repair of dna double-strand breaks (dsb) was studied in cho cells after 60 gy of x-rays using constant-field gel electrophoresis. heat given prior to irradiation was found not to alter the number of dsb when measured immediately after irradiation. in non-heated cells, about 80% of all dsb were rapidly repaired with a half-time of 4 min, while 20% were repaired more slowly with t4 = 160 min. these kinetics were grossly altered by heat. ...	1997	9269310
advances in molecular genetics of alpha-2- and alpha-3/4-fucosyltransferases.	fucosyltransferases are involved in the last steps of the biosynthesis of abh and lewis oligosaccharide antigens. seven human genes (fut1 to fut7) and one pseudogene (sec 1) have been cloned and localized on different chromosomes (9q34.3; 11q21; 19p13.3 and 19q13.3). their locations and their high degree of primary sequence identity, suggest that they have appeared by successive duplications followed by translocation and divergent evolution. their expression is tissue specific and they present a ...	1997	9269717
preoperative chemotherapy versus surgical therapy alone for squamous cell carcinoma of the esophagus: a prospective randomized trial.	this study investigated the role of preoperative chemotherapy in squamous cell cancer of the esophagus.	1997	9270638
clusters of s1 nuclease-hypersensitive sites induced in vivo by dna damage.	dna end-labeling procedures were used to analyze both the frequency and distribution of dna strand breaks in mammalian cells exposed or not to different types of dna-damaging agents. the 3' ends were labeled by t4 dna polymerase-catalyzed nucleotide exchange carried out in the absence or presence of escherichia coli endonuclease iv to cleave abasic sites and remove 3' blocking groups. using this sensitive assay, we show that dna isolated from human cells or mouse tissues contains variable basal ...	1997	9271420
frequency and necessity of thyroid function tests in neonates and infants with congenital hypothyroidism.	the american academy of pediatrics recommends frequent thyroid function tests in infants and children with congenital hypothyroidism (ch). data supporting the recommended frequency are lacking. this review was conducted to assess the validity of these recommendations.	1997	9271621
development of a technique for multiple site-directed mutagenesis of the ftf gene of streptococcus salivarius containing palindromic sequences.	attempts at site-directed mutagenesis of the fructosyltransferase (ftf) gene of streptococcus salivarius atcc 25975 using standard protocols were unsuccessful and resulted in a series of deletions. these deletions appeared to commence at points within the ftf gene where there were palindromic sequences which were capable of forming closed loop structures that acted as terminators under the conditions of mutagenesis. to overcome this problem, two modified mutagenic techniques were developed. they ...	1997	9271874
the value of endorectal ultrasound in the assessment of adenomas, t1- and t2-carcinomas.	in a prospective study we examined the value of endorectal ultrasound (erus) in the preoperative staging of potentially locally excisable tumours. during the study period from 1.1.1991 to 1.3.1996 a total of 160 rectal tumours in 152 patients were staged endosonographically (ut/un) and compared postoperatively with the histologic result (pt/pn) at the university hospital of würzburg. thirty-eight (24%) patients had an adenoma and 15 (9%) a t1-carcinoma. in 29 (18%) cases a t2-cancer was diagnose ...	1997	9272450
a rare type of overlapping genes in bacteriophage t4: gene 30.3' is completely embedded within gene 30.3 by one position downstream.	we have previously proposed the existence of one of the rarest types of overlapping genes in bacteriophage t4. we now present the results demonstrating that in a pair of t4 overlapping genes, 30.3 and 30.3', the smaller gene, 30.3', is entirely enclosed within the other by one position downstream. we have constructed plasmids in which different open reading frames from the gene 30.3 region were fused with the 5' end of the lacz beta-galactosidase (betagal) gene of escherichia coli. the gene fusi ...	1997	9272856
[radiotherapy in locoregional treatment of inoperable non-small cell lung cancer: results from a series of 381 patients].	the prognosis of inoperable non-small cell lung cancer (nsclc) is poor and thoracic radiation therapy is usually the main step of the therapeutic approach. the results of a retrospective analysis of a series of 381 patients treated from 1977 to 1990 for an inoperable nsclc are reported.	1997	9273183
[postoperative radiotherapy in non-small cell lung cancer. apropos of a series of 374 cases].	several randomized trials have led us to address the usefulness of post-surgical external beam therapy (ebt) in non-oat cell bronchial carcinoma. results that were obtained in a group of 374 patients submitted between 1977 and 1994 to identical therapy-the follow-up being done by the same team-and results of six randomized trials are analyzed.	1997	9273184
spo0a represses transcription of the cry toxin genes in bacillus thuringiensis.	the dna regions upstream from the genes encoding polypeptides of bacillus thuringiensis subsp. israelensis larvicidal crystals (cry4a, cry4b, cry11a) contain sequences with similarities to the spo0a box of bacillus subtilis (or '0a' box) and the promoter recognized by the sigma h-associated rna polymerase of b. subtilis. expression of cry-lacz transcriptional fusions was analysed in various b. thuringiensis genetic backgrounds. the early transcription of the toxin genes was not sporulation-depen ...	1997	9274027
genetic relationships among pasteurella trehalosi isolates based on multilocus enzyme electrophoresis.	genetic diversity among 60 british pasteurella trehalosi isolates representing the four recognized capsular serotypes, t3, t4, t10 and t15, and recovered predominantly from sheep suffering from systemic pasteurellosis, was estimated by analysing electrophoretically demonstrable allelic variation at structural genes encoding 19 enzymes. thirteen of the locl were polymorphic and 20 distinctive multilocus genotypes (electrophoretic types, ets) were identified. the population structure of p. trehalo ...	1997	9274038
impaired reproductive function in women treated for growth hormone deficiency during childhood.	there are limited data which suggest that disturbance of reproductive function may occur in gh-deficient women. we have evaluated the consequences of growth hormone (gh) deficiency on reproductive function in women treated for gh deficiency during childhood.	1997	9274698
altered bioavailability due to changes in the formulation of a commercial preparation of levothyroxine in patients with differentiated thyroid carcinoma.	in july 1995 we began noticing an unusually high rate of elevated tsh levels in patients with differentiated thyroid cancer treated with levothyroxine-specifically the brand levothroid-becoming more obvious from september 1995. faced with the possibility that these findings had some relationship to the drug taken, we carried out a prospective study, changing this brand of levothyroxine for another.	1997	9274701
the thyrotrophic role of human chorionic gonadotrophin (hcg) in the early stages of twin (versus single) pregnancies.	human chorionic gonadotrophin (hcg) is known to possess thyroid-stimulating activity. the aim of the present study was to assess the role of hcg in stimulating the maternal thyroid gland in the early stages of normal gestation.	1997	9274703
thyrotropin regulation by thyroid hormone in thyroid hormone receptor beta-deficient mice.	thyroid hormone responsive genes can be both positively and negatively regulated by thyroid hormone. tsh is down-regulated by thyroid hormone and rises during thyroid hormone deprivation. because both thyroid hormone receptor (tr) alpha and beta genes are expressed in the pituitary gland, it is unclear what the relative roles of tr alpha and tr beta are in tsh regulation. experiments using over expression of artificial genes have yielded conflicting results. the tr beta knock-out mouse that lack ...	1997	9275045
extrathyroidal effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on thyroid hormone turnover in male sprague-dawley rats.	treatment of rats with different polyhalogenated aromatic hydrocarbons strongly decreases plasma t4, with little or no decrease in plasma t3. the extrathyroidal effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) on thyroid hormone turnover were studied by i.p. administration of a single dose of 10 microg tcdd/kg bw or vehicle (corn oil) to euthyroid (eu) rats, thyroidectomized (tx) rats, and tx rats infused with 1 microg t4 (tx+t4) or 0.4 microg t3 (tx+t3)/100 g bw x day by osmotic minipumps. ...	1997	9275058
triiodothyronine (t3) reflects renal graft function after renal transplantation.	abnormalities in thyroid function are observed in patients with end stage renal disease. however, there are no data available evaluating sequential changes of thyroid function after renal transplantation. therefore, we have studied thyroid hormone function in the immediate post-operative period after renal transplantation in order to determine the relationship between improving renal function and changes in thyroid hormone economy.	1997	9231052
components of biological variation, including seasonality, in blood concentrations of tsh, tt3, ft4, prl, cortisol and testosterone in healthy volunteers.	there are few detailed studies of annual or seasonal variations in hormone concentrations in man. this study examines the components of biological variation, including seasonality, in plasma tsh, total t3 (tt3), free t4 (ft4), prl, cortisol and testosterone in healthy volunteers.	1997	9231055
influence of an antiprogestin (onapristone) on in vivo and in vitro fertilization.	the effects of a progesterone antagonist (onapristone) on heat synchronization, luteinizing hormone (lh) surge, ovulation, oocyte maturation and fertilization of superovulated ewes were studied. its effects on in vitro bovine oocyte maturation and fertilization were also studied. estrus synchronization and superovulation treatments were applied to 39 adult ewes using an intravaginal sponge with fluorgestone acetate for 9 days with injections of prostaglandin f2 alpha and pregnant mare's serum go ...	1997	9231247
fractionated high-dose-rate and pulsed-dose-rate brachytherapy: first clinical experience in squamous cell carcinoma of the tonsillar fossa and soft palate.	fractionated high-dose-rate (fr.hdr) and pulsed-dose-rate (pdr) brachytherapy (bt) regimens, which simulate classical continuous low-dose-rate (ldr) interstitial radiation therapy (irt) schedules, have been developed for clinical use. this article reports the initial results using these novel schedules in squamous cell carcinoma (scc) of the tonsillar fossa (tf) and/or soft palate (sp).	1997	9231672
external beam radiation therapy for squamous cell carcinoma of the soft palate.	external beam radiation therapy for carcinoma of the soft palate aims to achieve loco-regional control with normal speech, nasal function, swallowing mechanism, and minimal side effects such as nasal speech and regurgitation of food into the nasopharynx. in this report we present our results of radiotherapy in the treatment of 24 patients with squamous cell carcinoma of the soft palate.	1997	9231673
lack of effect of thyroid hormone on late fetal rat brain development.	studies were undertaken to test whether alterations in fetal brain thyroid hormone levels during the final week of gestation can prematurely induce gene expression in brain or affect cerebellar morphogenesis. pregnant dams were treated either by administration of 0.025% methimazole (mmi) in the drinking water from day 14 post conception (pc14) or administration of 2.5 mg t4/100 g bw on pc15. on pc21, treatment with mmi resulted in a 53% fall in fetal brain t3 levels and excess t4 resulted in a 2 ...	1997	9231758
3,5-diiodo-l-thyronine stimulates type 1 5'deiodinase activity in rat anterior pituitaries in vivo and in reaggregate cultures and gh3 cells in vitro.	local deiodination of l-thyroxine (t4) to the active thyroid hormone t3 via two 5'deiodinase isoenzymes (5'di and 5'dii) plays an important role for various t3-dependent functions of the anterior pituitary (ap). recently, it was reported that 3,5-t2, the 5'deiodination product of t3, acts as a specific agonist in the feedback mechanism on tsh secretion at the pituitary level. we now examined the effects of 3,5-t2 on pituitary 5'deiodinase activities in vivo in male, adult rats and in vitro using ...	1997	9231774
regional distribution of type 2 thyroxine deiodinase messenger ribonucleic acid in rat hypothalamus and pituitary and its regulation by thyroid hormone.	to identify the specific locations of type 2 deiodinase (d2) messenger rna (mrna) in the hypothalamus and pituitary gland and determine its regulation by thyroid hormone, we performed in situ hybridization histochemistry, northern analysis, and quantitative rt-pcr in euthyroid, hypothyroid, and hyperthyroid rats. by in situ hybridization histochemistry, silver grains were concentrated over ependymal cells lining the floor and infralateral walls of the third ventricle extending from the rostral t ...	1997	9231788
a critical period for thyroid hormone action on seasonal changes in reproductive neuroendocrine function in the ewe.	thyroid hormones are obligatory for the annually recurring termination of reproductive activity in a spectrum of seasonal breeders, including sheep. previous studies involving thyroidectomy and t4 replacement have led to the hypothesis that, in the ewe, thyroid hormones are necessary only during a limited interval late in the breeding season for the neuroendocrine processes that cause the transition to anestrus. the present series of experiments tested this hypothesis by assessing the influence ...	1997	9231794
effects of a 110 kilometers ultra-marathon race on plasma hormone levels.	to assess changes of sex hormones, cortisol, prolactin (prl) and beta-endorphins in an exhaustive aerobic performance, blood samples were taken in 11 endurance trained runners (r) along an ultra-marathon race of 110 km (t1 before the start, t2 at km 33, t3 at km 75 and t4 immediately after completing the race). results were compared to a control group (c) who followed the race. cortisol (p < 0.001) and beta-endorphins (p = 0.009) showed a significant increase during the race without significant ...	1997	9231840
an invariant lysine residue is involved in catalysis at the 3'-5' exonuclease active site of eukaryotic-type dna polymerases.	a lysine residue, contained in the motif "kx2h", has been invariantly found in the eukaryotic-type (family b) class of dna-dependent dna polymerases with a proofreading function. the importance of this lysine has been assessed by site-directed mutagenesis in the corresponding residue (lys143) of phi29 dna polymerase. substitution of this residue either by arginine or isoleucine severely impaired the catalytic efficiency of the 3'-5' exonuclease activity, giving a characteristic distributive patt ...	1997	9231901
orthostatic hypotension in a case with multiple sclerosis.	a 37-year-old woman with a 5-year history of multiple sclerosis is reported. she began having recurrent syncope even in the sitting position; other neurological features included hiccup, faciooro-lingual flushing and clumsiness of the hands. she had alternating horner's syndrome, mild hypoalgesia of the right face, exaggerated deep tendon reflexes of the upper extremities, decreased deep sensation and ataxia of the upper extremities, and incomplete transverse myelopathy with a t4 sensory level. ...	1997	9232362
morphometric changes in the prepubertal female rat thyroid gland following acute exposure to 2,2',4,4'-tetrachlorobiphenyl and aroclor 1242.	weanling female sprague-dawley rats were given either 2 or 5 consecutive daily doses of 30 mg/kg 2,2',4,4'-tetrachlorobiphenyl (cb 47) or a total dose of 120 mg/kg aroclor 1242 divided into 2, 3, or 5 daily doses by intraperitoneal injection. one day after the final dose, serum total thyroxine (t4) was determined and thyroid glands were collected and prepared for morphometric analysis. serum t4 increased between 20 and 25 d of age, but declined to 35-52% of controls by d 25 in pcb-treated rats. ...	1997	9233383
bacteriophage t4 uvsw protein is a helicase involved in recombination, repair and the regulation of dna replication origins.	bacteriophage t4 uvsw protein is involved in phage recombination, repair and the regulation of replication origins. here, we provide evidence that uvsw functions as a helicase. first, expression of uvsw allows growth of an (otherwise inviable) escherichia coli recg rnha double mutant, consistent with uvsw being a functional analog of the recg helicase. second, uvsw contains helicase sequence motifs, and a substitution (k141r) in the walker 'a' motif prevents growth of the e.coli recg rnha double ...	1997	9233823
[cancer of the larynx in ecuador].	a retrospective review was made of 75 cases of laryngeal cancer evaluated and treated at the social security hospital of quito (ecuador). no exclusively supraglottic lesions were found; 67% of cases, were glottic or glottic-supraglottic. thirty-two per cent of cases were t1 and t2 and 23% had palpable lymph nodes in neck. most t1 and t2 tumors were treated with radiotherapy (rt) and most t3 and t4 tumors with a combination of surgery and rt. the 5 and 10-year overall survival rates were 55% and ...	1997	9235036
[how successful is the filtering bleb "needling"?].	"needling" may become necessary when filtering blebs fail due to scarring or encapsulation. our goal was to calculate the medium term success rate of the needling procedure.	1997	9235392
effects of thyrotropin-releasing hormone and its analogs on daytime sleepiness and cataplexy in canine narcolepsy.	the therapeutic potential of thyrotropin-releasing hormone (trh) and trh analogs in narcolepsy, a sleep disorder characterized by abnormal rapid eye movement (rem) sleep and daytime sleepiness, was examined using the canine model. the effects of trh and the biologically stable trh analogs cg3703, cg3509, and ta0910 on daytime sleep and cataplexy, a symptom of abnormal rem sleep, were assessed using polysomnographic recordings and the food elicited cataplexy test (fect), respectively. cg3703 (100 ...	1997	9236248
prognostic factors in carcinoma of the external auditory canal.	carcinomas of the external auditory canal are rare neoplasms (< 1% of all head and neck malignant neoplasms).	1997	9236591
neoadjuvant intra-arterial polychemotherapy of locally advanced rectal cancer.	in order to evaluate the usefulness of pre-operative intra-arterial selective polychemotherapy (piasp), we carried out a retrospective study of 107 patients (65 males, 42 females) with locally advanced rectal cancer (larc) (pt3-4 n0-1 m0), who were treated between 1988 and 1991. fifty-two patients (mg) underwent piasp (adriablastin 60-90 mg, fluorouracil 3-4 g) with subsequent radical surgery. fifty-five patients (r0) received surgery alone. angiographic findings after piasp showed approximately ...	1997	9236897
surgically curable and incurable scirrhous carcinomas of the stomach.	the purpose of this study was to identify a subgroup of patients with scirrhous carcinoma of the stomach who are more suitable for surgery by analysis of their clinicopathologic characteristics.	1997	9236929
superior detachment of a glenoid labrum variant resembling an incomplete discoid meniscus in a wheelchair ambulator.	symptoms of instability and painful clunking in the shoulder were found associated with superior detachment of an incomplete discoid glenoid labrum and biceps origin in a 25-year-old woman. the patient was paraplegic below the t4 level secondary to resection of a spinal cord astrocytoma at age of 17. symptoms began after 3 years of wheelchair use and repetitive transfer injuries. on arthroscopic examination, only the central surface of the articular glenoid cartilage was visible because of labra ...	1997	9276061
long-term regional control after radiation therapy and neck dissection for base of tongue carcinoma.	minimal literature exists with 10-year data on neck control in advanced head and neck cancer. the purpose of this study is to determine long-term regional control for base of tongue carcinoma patients treated with primary radiation therapy plus neck dissection.	1997	9276364
unexpectedly general replaceability of atp in atp-requiring enzymes.	atp-dependent enzymes were investigated as to the stringency of their atp requirement. for all the enzymes examined except firefly luciferase (including hexokinase, polynucleotide kinase, t4 dna ligase, and t4 rna ligase) atp could be replaced with datp, contradicting previous data. considering the replaceable nucleotides, not only kinases (low stringency as to atp-requirement) but also other enzymes (moderate stringency) were typed as phosphate-directed atp recognition. through this study, an e ...	1997	9276690
photoreaction of new psoralen analogs with dna: sequence and mutation specificity in the escherichia coli lacz gene.	new thio- and seleno-analogs of psoralen were synthesized and analyzed for their photoreactivity toward dna. using oligonucleotides of defined sequence, we first showed that these derivatives predominantly generated interstrand crosslinks at 5'-tpa sites. we also observed a surprisingly high reactivity of 7h-thiopyrano[3,2-f][1]benzofuran-7-one (pso[o-s]) with the bamhi and psti oligomers, giving rise to the formation of crosslinks at 5'-apt sites and of the thymidine-psoralen-cytosine type. nex ...	1997	9277140
the pharmacokinetics and pharmacodynamics of rocuronium in patients with hepatic cirrhosis.	to determine the effects of hepatic cirrhosis on the pharmacodynamics and pharmacokinetics of rocuronium bromide.	1997	9278198
fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded dna/rna using t4 rna ligase.	a rapid 5'-labeling method of single-stranded dna/rna was developed, which is based on the utilization of an adenylated intermediate in the reaction of t4 rna ligase. this method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleotides.	1997	9278501
treatment of rats with dexamethasone or thyroxine reverses zinc deficiency-induced intestinal damage.	structural and functional damage to the intestine and the potential beneficial effects of dexamethasone (dex) and thyroxine (t4) were examined in zinc-deficient rats. rats were assigned to zinc deficient (zd), control (c) or pair-fed (pf ) groups and fed for 40 d a zinc deficient (1 mg/kg) diet (zd rats) or a similar diet supplemented with 50 mg zn/kg (c and pf rats). some rats of the zd group were treated for the last 10 d with low (250 mg/kg) or high (5 mg/kg) doses of dex or with t4 (100 mg/k ...	1997	9278564
prenatal glucocorticoid and t4 effects on lung morphology in preterm lambs.	prenatal glucocorticoid plus t4 treatment of fetal sheep results in improvements in oxygenation, gas exchange, lung mechanics, and lung volumes after preterm delivery. we have evaluated the morphometric changes in the lungs of lambs exposed to betamethasone and t4 48 h before preterm delivery at 121 and 135 d gestation and related those changes to the physiologic improvements in lung function. the lungs used for the morphometric studies were from lambs with postnatal physiologic responses simila ...	1997	9279249
[intracavitary brachytherapy in esophageal neoplasms. experience at the radiology institute of brescia].	we investigated the role of brachytherapy in the management of esophageal cancer. from march, 1990, to december 1994, seventy patients, with biopsy-proved esophageal carcinoma, were treated with brachytherapy +/- external beam radiotherapy. according to the tnm staging system, 24 patients were graded as t1, 24 as t2, 10 as t3, 9 as t4, and 3 as tx. median karnofsky score was 80. dysphagia was the major symptom in 68% of cases. treatment consisted of low dose rate first, and then high dose rate b ...	1997	9280947
tetraparesis due to vertebral physeal fracture in an adult dog with congenital hypothyroidism.	a four-year-old male affenpinscher was referred for evaluation of hindlimb weakness that had progressed to tetraparesis over a period of four weeks. neurological examination was suggestive of a cervical spinal cord lesion. radiographic examination revealed diffuse skeletal immaturity with open physes and epiphyseal dysplasia in long bones and vertebrae, consistent with a diagnosis of congenital hypothyroidism. total and free serum t4 concentrations were very low, indicative of hypothyroidism. su ...	1997	9282344
a member of the immunoglobulin superfamily in bacteriophage t4.	we report a prediction that the highly immunogenic outer capsid (hoc) protein of the prokaryotic phage t4 contains three tandem immunoglobulin-like domains. immunoglobulin-like folds have previously been identified in prokaryotic proteins but these share no recognizable sequence similarity with eukaryotic immunoglobulin superfamily (igsf) folds, and may represent products of convergent evolution. in contrast, the hoc immunoglobulin-like folds are proposed, based on immunoglobulin-like sequence c ...	1997	9237357
rapid method for detecting desulfitobacterium frappieri strain pcp-1 in soil by the polymerase chain reaction.	a rapid method was developed for detecting in soil desulfitobacterium frappieri strain pcp-1, an anaerobic gram-positive bacterium, isolated from a methanogenic consortium degrading pentachlorophenol. the method involved the establishment of a protocol for extracting total dna from soil with the least contamination, and the use of the polymerase chain reaction (pcr) to detect strain pcp-1 with primers targeted with pcp-1 16s rrna. to optimize the dna extraction conditions, a glass mill homogeniz ...	1997	9237393
preoperative chemotherapy for stage iiia non-small cell lung cancer.	recent studies have shown that preoperative chemotherapy is feasible and advisable for patients with stage iiia mediastinoscopically confirmed n2 involvement in non-small cell lung cancer. such studies confirm the crucial pillar of attaining complete resection, and the importance of downstaging n2 disease as a useful surrogate for long-term survival. resectability rate is about 70% in almost all studies examined. better means of predicting resectability and relapse are addressed, such as the pot ...	1997	9161793
effect of thyroid hormone on urinary excretion of epidermal growth factor.	thyroid hormones were demonstrated to upregulate the synthesis of epidermal growth factor (egf) mrna and proegf in the kidneys in vivo. this prospective study was performed to examine the extent of the hypothyroid state in modulating egf excretion in the urine (u-egf; n = 23). patients with thyroid cancer were serially followed up from the euthyroid status, which was retained by thyroxine supplement after thyroidectomy, to the hypothyroidism in preparing for 131i whole body scanning. paired thyr ...	1997	9161839
motor power pharmacodynamics of subarachnoid hyperbaric 5% lidocaine in the sitting position.	repetitive dynamometric measurement using a plantar flexion power device (pfpd) provides detailed data describing the onset and offset of motor block following spinal administration of lidocaine. the aim of this study was to evaluate administration of two doses of spinal lidocaine in the sitting position to determine whether our dynamometric model produces data consistent with our current understanding of the pharmacokinetics of subarachnoid, hyperbaric, 5% lidocaine.	1997	9181155
supplemental thyroid hormones and molting in turkey breeder hens.	the objective of the current study was to determine whether thyroid physiology may affect molting time in turkeys. two trials using approximately 144 hens were conducted to elucidate thyroidal factors that limit the molting process. thyroid hormones or a thyroid blocker (thiouracil) were given to the hens during a molt by supplementing the diet with thyroxine (t4), triiodothyronine (t3), or thiouracil. supplementing with t4 reduced the number of days to return to egg production, whereas suppleme ...	1997	9181624
the direct effects of thyroid hormones on rat mesenteric resistance arteries.	the direct relaxant effects of thyroid hormones on mesenteric resistance vessels were investigated using an isometric wire myograph. both the l- and the d-isomers of thyroxine (t4) and triiodothyronine (t3) were studied. in contrast with the long-term effects of thyroid hormones, both t4 enantiomers proved more potent in inducing vascular relaxation than the two t3 enantiomers. the interaction between thyroid hormones and calcium-induced contractions was studied. t4 concentration dependently inh ...	1997	9182075
identification of amino acids of endonuclease vii essential for binding and cleavage of cruciform dna.	endonuclease vii is a holliday-structure-resolving enzyme of bacteriophage t4. the active protein is a homodimer with 157 amino acids/monomer. an amber mutation (ame727 in codon 151) inactivates the nuclease completely, indicating the importance of the seven c-terminal amino acids for nucleolytic activity. the influence of these amino acids on cruciform-dna binding and cleavage was investigated through functional analysis of c-terminal-truncated proteins derived from deletion constructs. it was ...	1997	9182992
rapid cycling bipolar affective disorder: lack of relation to hypothyroidism.	thyroid indices were measured after an extended period of medication-free evaluation averaging 6 weeks in 67 consecutively admitted patients with bipolar illness. thyroid hormone levels -- thyroxine (t4), free t4 and triiodothyronine (t3) -- were not significantly different in the 31 rapid cyclers (> or = 4 affective episodes/year) than in 36 non-rapid cyclers. analysis of covariance indicated a non-significant trend relation between higher t4 and a greater number of affective episodes in the ye ...	1997	9355813
down-regulation of tsh subunit mrna levels by thyroid hormones in the european eel.	the effects of 3,5,3'-triiodothyronine (t3) and thyroxine (t4) on alpha and thyroid-stimulating hormone (tsh) beta subunit mrna pituitary levels were examined in a teleost, the european silver eel. northern blot analysis showed that the number and length of mrnas encoding tsh beta varied among individuals, a variability apparently not related to thyroidal status. when several bands were present, their intensities were summed for quantitative analysis. increasing circulating thyroid hormones (ths ...	1997	9356215
plasma gonadotropin ii, sex steroids, and thyroid hormones in wild striped bass (morone saxatilis) during spermiation and final oocyte maturation.	the blood levels of gonadotropin ii (gth ii), sex-steroid hormones, and thyroid hormones were determined in wild spermiating male striped bass (morone saxatilis) in males and in females at various stages of final oocyte maturation (fom), captured on their spawning grounds. the progression of spermiation was associated with increases in plasma gth ii and decreases in plasma testosterone (t), 11-ketotestosterone, and thyroxine (t4). plasma triiodothyronine (t3) remained at high and relatively unch ...	1997	9356218
effect of melatonin on the response of the thyroid to thyrotropin stimulation in vitro.	thyroidal-melatonin interactions are of particular importance to amphibian development since the thyroid controls the progress of metamorphosis while melatonin may coordinate its rate with prevailing environmental conditions. melatonin antagonized thyroxine (t4) action at the tissue level and directly inhibited baseline t4 secretion in culture, so the present work sought to determine if it antagonized the response of the thyroid to thyroid stimulating hormone (tsh) as well. a preliminary experim ...	1997	9356225
protein synthesis-dependent potentiation by thyroxine of antiviral activity of interferon-gamma.	we have studied the prenuclear signal transduction pathway by which thyroid hormone potentiates the antiviral activity of human interferon-gamma (ifn-gamma) in hela cells, which are deficient in thyroid hormone receptor (tr). the action of thyroid hormone was compared with that of milrinone, which has structural homologies with thyroid hormone. l-thyroxine (t4), 3,5,3'-l-triiodothyronine (t3), and milrinone enhanced the antiviral activity of ifn-gamma up to 100-fold, a potentiation blocked by cy ...	1997	9357766
thyroid function in very preterm infants: influences of gestational age and disease.	it is not known how immaturity and disease influence postnatal thyroid function in infants <30 wk of gestational age. we performed serial measurements of plasma thyroxine (t4), free t4 (ft4), triiodothyronine (t3), reverse t3 (rt3), tsh, and t4-binding globulin (tbg) in 100 infants of <30 wk of gestation, during the first 8 postnatal weeks, to investigate the influences of disease and gestational age on the time course of thyroid hormones. one hundred infants were divided twice into two groups: ...	1997	9357931
non-homologous dna end joining in plant cells is associated with deletions and filler dna insertions.	double strand dna breaks in plants are primarily repaired via non-homologous end joining. however, little is known about the molecular events underlying this process. we have studied non-homologous end joining of linearized plasmid dna with different termini configurations following transformation into tobacco cells. a variety of sequences were found at novel end junctions. joining with no sequence alterations was rare. in most cases, deletions were found at both ends, and rejoining usually occu ...	1997	9358178
effects of thyroxine (t4) or triiodothyronine (t3) replacement therapy on the programming of seasonal reproduction and postnuptial molt in thyroidectomized male american tree sparrows (spizella arborea) exposed to long days.	this study tested the hypothesis that t3 (triiodothyronine) is the tissue-active "seasonality" hormone by determining whether t3 could mimic t4 (thyroxine) and program photostimulated thyroidectomized (thx) male american tree sparrows (spizella arborea) for three components of seasonality (i.e., full-blown testicular growth, photorefractoriness, and postnuptial molt). photosensitive males were radiothyroidectomized, transferred to long days 4 weeks later, and administered 14 daily injections (s. ...	1997	9360316
effects of propylthiouracil and methimazole on fetal thyroid status in mothers with graves' hyperthyroidism.	thionamide therapy is a mainstay of the treatment of hyperthyroidism complicated by pregnancy, but it can expose the fetus to hypothyroidism. in terms of fetal thyroid status, propylthiouracil (ptu) has been preferred over methimazole (mmi) based on experimental data on limited transplacental passage, and lower doses have been recommended. however, neither of these practices is supported by convincing clinical evidence. we compared the effect of maternal ingestion of ptu with that of mmi on feta ...	1997	9360518
long-term results of preoperative radiation therapy alone for stage t3 and t4 rectal cancer.	there has been a resurgence of interest in the use of preoperative radiation therapy, with or without chemotherapy, for locally advanced rectal cancer. the purpose of this study was to analyse the time course and pattern of failure for 74 patients with clinical stage t3 or t4 (ct3-4) rectal cancer treated with preoperative radiation therapy for whom long-term follow-up was available.	1997	9361610
effects of high-dose gentamicin sulfate on neuromuscular blockade in halothane-anesthetized horses.	to evaluate effects of a single high dose of gentamicin on neuromuscular function in horses anesthetized with halothane.	1997	9361900
paracrine interaction between hepatocytes and macrophages after extrathyroidal proteolysis of thyroglobulin.	thyroglobulin (tg), the precursor of the thyroid hormones triiodothyronine (t3) and thyroxine (t4), is known to derive from thyroid epithelial cells. part of tg reaches the circulation as an intact molecule by transcytosis across the epithelial wall of thyroid follicles. circulating tg is a potential ligand for the asialoglycoprotein receptor of hepatocytes. in this report we show, however, that clearance of circulating tg occurred exclusively by endocytosis in liver macrophages, whereas hepatoc ...	1997	9362367
udp-gt involvement in the enhancement of cell proliferation in thyroid follicular cell proliferative lesions in rats treated with thiourea and vitamin a.	the mechanisms underlying enhanced cell proliferation in thyroid proliferative lesions of rats simultaneously treated with large amounts of vitamin a (va) and thiourea (tu) were investigated. male f344 animals were initiated with n-bis(2-hydroxypropyl)nitrosamine (2800 mg/kg body weight, single s.c. injection). starting 1 week later, groups received water containing 0.2% tu (tu group), diet containing 0.1% va (va group), both 0.2% tu and 0.1% va (tu + va group) or tap water/basal diet without su ...	1997	9363838
experimental hypothyroidism increases immobility in rats in the forced swim paradigm.	effects of severe and mild hypothyroidism on the immobile response to inescapable stress were examined in male wistar rats using the forced swim paradigm. rats were exposed to two sessions of inescapable swim stress: pretest (for 15 min) followed by test (for 5 min) 24 h later. surgically thyroidectomized rats showed a significant increase (by 90%) in immobility during test compared to sham rats. chronic administration of high (200 micrograms/kg per day) but not low (15 micrograms/kg per day) do ...	1997	9364510
multiple sclerosis presenting as a spinal cord tumor.	multiple sclerosis, a disorder of central nervous system demyelination, is a leading cause of disability in young people. lesions of the spinal cord are usually less than two vertebral body segments long, peripherally located, and found in the cervical region. a 30-year-old woman had a 2-month history of back pain, urinary incontinence, and bilateral lower extremity weakness. magnetic resonance imaging (mri) of the spine showed an intramedullary spinal cord tumor from t4 to t8 with an intramedul ...	1997	9365361
thyroxine, thyrotropin, and age in a euthyroid hospital patient population.	the diagnosis of thyroid disease now often can be achieved reliably by measuring thyrotropin (tsh) alone. thyroxine (t4), triiodothyronine, and other analytes are only needed if tsh and the accompanying clinical condition are discordant. we describe here work that confirms the age independence of tsh in both inpatient and outpatient euthyroid hospital populations between ages 20 and at least 80 years, and demonstrates that although free t4 does vary with age, the range of variation remains withi ...	1997	9365400
differential response of legumes and creep feeding on gut morphology and faecal composition in weanling pigs.	the effects of creep feeding and different levels of soybean meal (sbm) and cowpea meal on the intestinal morphology and faecal characteristics were investigated in weaners. prior to the feeding trial, one group of piglets was creep-fed and the other noncreep-fed. the two groups of piglets were weaned at 28 days and randomly assigned to four different diets, the main protein sources of which were: t1--skimmed milk power (control); t2--31% soybean meal (high sbm), t3--15% soybean meal and 12% ski ...	1997	9366068
cryosurgical ablation of prostate cancer: patterns of cancer recurrence.	we determined the rate of biochemical and biopsy failure in relation to the prostate specific antigen (psa) nadir, the effect of neoadjuvant androgen blockade and the pattern of residual tumor after cryosurgical ablation of prostate cancer.	1997	9366345
aerobic metabolism and cardioventilatory responses in paraplegic athletes during an incremental wheelchair exercise.	the aims of the present study were: (1) to assess aerobic metabolism in paraplegic (p) athletes (spinal lesion level, t4-l3) by means of peak oxygen uptake (vo2peak) and ventilatory threshold (vt), and (2) to determine the nature of exercise limitation in these athletes by means of cardioventilatory responses at peak exercise. eight p athletes underwent conventional spirographic measurements and then performed an incremental wheelchair exercise on an adapted treadmill. ventilatory data were coll ...	1997	9367286
the high-mobility group protein t160 binds to both linear and cruciform dna and mediates dna bending as determined by ring closure.	the high-mobility group protein t160 was isolated by screening a phage library from a murine pre-b-cell line l1210. south-western experiments have previously shown that this protein binds to v-(d)-j recombination signal sequences, suggesting that it may be a sequence-specific dna-binding protein. however, neither gel-shift nor footprinting analyses have been successfully employed with the t160 protein, despite an extensive effort. in this study, the t160 protein or truncated forms made soluble t ...	1997	9367632