Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| neisseria gonorrhoeae effectively blocks hiv-1 replication by eliciting a potent tlr9-dependent interferon-α response from plasmacytoid dendritic cells. | clinical and epidemiological research provides evidence for a positive correlation between neisseria gonorrhoeae infection and hiv transmission; however, mechanistic studies examining this relationship have yielded conflicting results. to explore this interaction, we exposed ex vivo cultured peripheral blood cells from acute hiv(+) individuals to n. gonorrhoeae. unexpectedly, we observed a profound inhibition in hiv-1 replication in the ex vivo cultures, and this was recapitulated when periphera ... | 2010 | 20735437 |
| increase in neisseria meningitidis serogroup w135, niger, 2010. | 2010 | 20735947 | |
| the role of the exopolyphosphatase ppx in avoidance by neisseria meningitidis of complement-mediated killing. | the complement system is critical for immunity against the important human pathogen neisseria meningitidis. we describe the isolation of a meningococcal mutant lacking ppx, an exopolyphosphatase responsible for cleaving cellular polyphosphate, a polymer of tens to hundreds of orthophosphate residues found in virtually all living cells. bacteria lacking ppx exhibit increased resistance to complement-mediated killing. by site directed mutagenesis, we define amino acids necessary for the biochemica ... | 2010 | 20736171 |
| the structure of a reduced form of oxyr from neisseria meningitidis. | survival of the human pathogen, neisseria meningitidis, requires an effective response to oxidative stress resulting from the release of hydrogen peroxide by cells of the human immune system. in n. meningitidis, expression of catalase, which is responsible for detoxifying hydrogen peroxide, is controlled by oxyr, a redox responsive lysr-type regulator. oxyr responds directly to intracellular hydrogen peroxide through the reversible formation of a disulphide bond between c199 and c208 in the regu ... | 2010 | 20478059 |
| thermostable formulations of a hepatitis b vaccine and a meningitis a polysaccharide conjugate vaccine produced by a spray drying method. | thermostable vaccines promise to simplify the logistics of vaccine distribution and expand the immunization coverage. in this study, a pilot-scale spray drying process was developed and used to produce glassy state formulations of a recombinant hepatitis b (hepb) vaccine containing aluminum adjuvant and neisseria meningitidis a (mena) protein-polysaccharide conjugate vaccine, representing two common types of subunit vaccines in use today: the spray-dried hepb vaccine formulations were stable for ... | 2010 | 20478345 |
| 2010 fifa world cup south africa: travel health issues and new options for protection against meningococcal disease. | the public health implications of large crowds gathering at a range of key global events should never be underestimated. this is especially the case with the upcoming 2010 fifa world cup south africa programme where thousands of local and travelling spectators, players and officials from all over the world will be present. although meningococcal disease contracted whilst actually travelling is relatively rare, any travel health risk assessment should involve consideration of potential exposure t ... | 2010 | 20478511 |
| the oligosaccharide of gonococcal lipooligosaccharide contains several epitopes that are recognized by human antibodies. | recently, we isolated human igg from normal human sera (nhs) using lipooligosaccharide (los) from gonococcal strain jw31r as an affinity ligand. we provided evidence that the oligosaccharide (os) moiety of los was immunogenic in humans and that nhs contains functional antibodies that bind to the branched os. the present study aimed to identify bactericidal antibodies that bind to partial core os structures or their adjacent sites expressed in the 3,4-branched and 2,3:3,4-dibranched neisserial lo ... | 2010 | 20479085 |
| multiple pilus motors cooperate for persistent bacterial movement in two dimensions. | in various bacterial species surface motility is mediated by cycles of type iv pilus motor elongation, adhesion, and retraction, but it is unclear whether bacterial movement follows a random walk. here we show that the correlation time of persistent movement in neisseria gonorrhoeae increases with the number of pili. the unbinding force of individual pili from the surface f=10 pn was considerably lower than the stalling force f>100 pn, suggesting that density, force, and adhesive properties of t ... | 2010 | 20482147 |
| cervicovaginal fluid and semen block the microbicidal activity of hydrogen peroxide produced by vaginal lactobacilli. | h2o2 produced by vaginal lactobacilli is believed to protect against infection, and h2o2-producing lactobacilli inactivate pathogens in vitro in protein-free salt solution. however, cervicovaginal fluid (cvf) and semen have significant h2o2-blocking activity. | 2010 | 20482854 |
| spectinomycin in the treatment of gonorrhoea. | 2010 | 20483105 | |
| improved omv vaccine against neisseria meningitidis using genetically engineered strains and a detergent-free purification process. | the use of detergent-extracted outer membrane vesicles (omvs) is an established approach for development of a multivalent pora vaccine against n. meningitidis serogroup b. selective removal of lipopolysaccharide (lps) decreases toxicity, but promotes aggregation and narrows the immune response. detergent-free omv vaccines retain all lps, which preserves the native vesicle structure, but result in high toxicity and lower yield. the present study assessed the effects of gene mutations that attenua ... | 2010 | 20483197 |
| structural and biochemical characterization of free methionine-r-sulfoxide reductase from neisseria meningitidis. | a new family of methionine-sulfoxide reductase (msr) was recently described. the enzyme, named frmsr, selectively reduces the r isomer at the sulfoxide function of free methionine sulfoxide (met-r-o). the frmsrs belong to the gaf fold family. they represent the first gaf domain to show enzymatic activity. two other msr families, msra and msrb, were already known. msra and msrb reduce free met-s-o and met-r-o, respectively, but exhibit higher catalytic efficiency toward met-o within a peptide or ... | 2010 | 20489204 |
| identification and characterization of important residues in the catalytic mechanism of cmp-neu5ac synthetase from neisseria meningitidis. | sialylated oligosaccharides, present on mammalian outer-cell surfaces, play vital roles in cellular interactions and some bacteria are able to mimic these structures to evade their host's immune system. it would be of great benefit to the study of infectious and autoimmune diseases and cancers, to understand the pathway of sialylation in detail to enable the design and production of inhibitors and mimetics. sialylation occurs in two stages, the first to activate sialic acid and the second to tra ... | 2010 | 20491913 |
| measuring antigen-specific bactericidal responses to a multicomponent vaccine against serogroup b meningococcus. | serum bactericidal activity using human complement is the basis for established correlates of protection against invasive meningococcal disease. during the development of multicomponent protein-based vaccines against meningococcus b, it is necessary to measure antigen-specific bactericidal responses. this is not straightforward because each strain may be killed by antibodies to multiple antigens. we characterized a large panel of strains and, using a competitive inhibition sba, we identified fou ... | 2010 | 20493284 |
| genetic structure of neisseria meningitidis populations. | 2010 | 20493968 | |
| role of citrate and phosphate anions in the mechanism of iron(iii) sequestration by ferric binding protein: kinetic studies of the formation of the holoprotein of wild-type fbpa and its engineered mutants. | ferric binding protein a (fbpa) plays a central role in the iron acquisition processes of pathogenic neisseria gonorrheae, neisseria meningitidis, and haemophilus influenzae. fbpa functions as an iron shuttle within the periplasmic space of these gram-negative human pathogens. iron is picked up by fbpa at the periplasmic aspect of the outer membrane with concomitant acquisition of a synergistic anion. here we report the kinetics and mechanisms involved with loading of iron(iii) into iron-free fb ... | 2010 | 20496864 |
| multiplex polymerase chain reaction-based reverse line blot hybridization assay to detect common genital pathogens. | the objective of the present paper is to develop and apply a multiplex polymerase chain reaction (mpcr) based reverse line blot (rlb) hybridization assay to facilitate the diagnosis of genital infections by detection of seven recognized or putative genital pathogens (neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma urealyticum, ureaplasma parvum, mycoplasma genitalium, mycoplasma hominis and trichomonas vaginalis). species-specific biotin-labelled primer pairs were used in a single mpcr ... | 2010 | 20498099 |
| risk of chlamydia trachomatis infection during pregnancy: effectiveness of guidelines-based screening in identifying cases. | our goal was to define the risk factors for chlamydia trachomatis (ct) infection among pregnant women at a large urban medical centre. in a retrospective study, clinical records at a us maternity unit from july 2005 through february 2008 were reviewed. the study population included all pregnant women with a singleton newborn of at least 20 weeks gestation and antenatal care information. logistic regression was used to analyse the association between a positive ct test and demographic, behavioura ... | 2010 | 20498110 |
| [the effectiveness and safety of vaccine against meningococci group c conjugated with tetanus toxoid co-administrated with different vaccines--literature review]. | review of articles on efficacy and safety of co-administration of neisseria meningitidis serogroup c -tetanus toxoid conjugate vaccine (neisvac-c) with other vaccines. | 2010 | 20499668 |
| the effectiveness of patient-delivered partner therapy and chlamydial and gonococcal reinfection in san francisco. | patient-delivered partner therapy (pdpt) has been evaluated in randomized trials. no analysis has examined the impact of pdpt once implemented programmatically. | 2010 | 20502392 |
| etiology and sti/hiv coinfections among patients with urethral and vaginal discharge syndromes in south africa. | this study was undertaken to establish the etiology of the male urethral discharge (muds) and vaginal discharge (vds) syndromes, to determine the prevalence of other sexually transmitted infections (sti) and human immunodeficiency virus (hiv) coinfections, and to examine associations between stis and hiv serostatus among sti patients in south africa. | 2010 | 20502394 |
| neisseria meningitidis opc invasin binds to the sulphated tyrosines of activated vitronectin to attach to and invade human brain endothelial cells. | the host vasculature is believed to constitute the principal route of dissemination of neisseria meningitidis (nm) throughout the body, resulting in septicaemia and meningitis in susceptible humans. in vitro, the nm outer membrane protein opc can enhance cellular entry and exit, utilising serum factors to anchor to endothelial integrins; but the mechanisms of binding to serum factors are poorly characterised. this study demonstrates that nm opc expressed in acapsulate as well as capsulate bacter ... | 2010 | 20502634 |
| [epidemiological, clinical and laboratory characteristics of bacterial meningitis in adult patients]. | to determine the epidemiological, clinical and laboratory characteristics of adult bacterial meningitis in the czech republic, including complications and causes of death. | 2010 | 20503157 |
| epidemiology and meningococcal serogroup distribution in the united states. | 2010 | 20507868 | |
| oip5 is a highly expressed potential therapeutic target for colorectal and gastric cancers. | previously, we reported that overexpression of opa (neisseria gonorrhoeae opacity-associated)-interacting protein 5 (oip5) caused multi-septa formation and growth defects, both of which are considered cancer-related phenotypes. to evaluate oip5 as a possible cancer therapeutic target, we examined its expression level in 66 colorectal cancer patients. oip5 was upregulated about 3.7-fold in tumors and over 2-fold in 58 out of 66 colorectal cancer patients. knockdown of oip5 expression by small int ... | 2010 | 20510019 |
| endotracheal tube biofilm inoculation of oral flora and subsequent colonization of opportunistic pathogens. | endotracheal (et) tubes accumulate a biofilm during use, which can harbor potentially pathogenic microorganisms. the enrichment of pathogenic strains in the biofilm may lead to ventilator-associated pneumonia (vap) with an increased morbidity rate in intensive care units. we used quantitative pcr (qpcr) and gene surveys targeting 16s rrna genes to quantify and identify the bacterial community to detect fastidious/nonculturable organisms present among extubated et tubes. we collected eight et tub ... | 2010 | 20510651 |
| reduced susceptibility to ceftriaxone in neisseria gonorrhoeae is associated with mutations g542s, p551s and p551l in the gonococcal penicillin-binding protein 2. | reduced susceptibility to extended-spectrum cephalosporins in neisseria gonorrhoeae has, to date, been associated with three alterations: a mosaic pena allele encoding the penicillin-binding protein 2 (pbp2); a-del-mtrr, an adenine deletion in the mtrr promoter; and penb, comprising mutated alleles of porbib. in this study, we examined an association between reduced susceptibility to ceftriaxone and additional mutations in gonococcal pbp2. | 2010 | 20511367 |
| neisseria gonorrhoeae fa1090 carries genes encoding two classes of vsr endonucleases. | a very short patch repair system prevents mutations resulting from deamination of 5-methylcytosine to thymine. the vsr endonuclease is the key enzyme of this system, providing sequence specificity. we identified two genes encoding vsr endonucleases v.ngoaxiii and v.ngoaxiv from neisseria gonorrhoeae fa1090 based on dna sequence similarity to genes encoding vsr endonucleases from other bacteria. after expression of the gonococcal genes in escherichia coli, the proteins were biochemically characte ... | 2010 | 20511499 |
| [correlation between predominant pi genotypes, g120/a121 mutations and drug resistance.]. | to analyze the predominant genotypes of outer membrane porin i (pi) and to determine the correlation between g120 as well as a121 mutations in pi proteins and drug resistance in neisseria gonorrhoeae isolates in the local area. | 2010 | 20513293 |
| meningococcal group a, c, y and w-135 conjugate vaccine. | in february 2010, a quadrivalent conjugate vaccine (menveo; novartis vaccines and diagnostics) was approved by the us fda to prevent invasive meningococcal disease caused by neisseria meningitidis serogroups a, c, y and w-135 in people aged 11-55 years. | 2010 | 20514064 |
| emergence of new virulent neisseria meningitidis serogroup c sequence type 11 isolates in france. | in france, there have been variations in the incidence of invasive meningococcal infection due to serogroup c isolates. infection peaks were observed in 1992 and 2003 that involved isolates of phenotypes c:2a:p1.5,2 and/or c:2a:p1.5, which belong to the sequence type 11 (st-11) clonal complex. we report an emergence of isolates belonging to the st-11 clonal complex since 2003. these isolates displayed a new phenotype, c:2a:p1.7,1, caused infections that occurred as clusters, and were associated ... | 2010 | 20515410 |
| absence of neisseria meningitidis from throat swabs of kuwaiti pilgrims after returning from the hajj. | to investigate whether or not neisseria meningitidis was present in the throat of kuwaiti pilgrims after returning from the hajj. | 2010 | 20516711 |
| [characteristics of microflora of laryngeal mucosa in healthy subjects and patients with cicatrical stenosis of the larynx]. | the authors present data on the occurrence, density, and species composition of microorganisms isolated from pharyngeal mucosa of 40 healthy subjects and 55 patients with cicatrical stenosis of the larynx. the total density of microbial populations in control subjects was estimated at 2,672+/-614 cfu/ml compared with 17,510+/-1,983 cfu/ml in patients with cicatrical stenosis of the larynx unrelated to malignant neoplastic growth and 19,375+/-2,103 cfu/ml in patients who had experienced surgical ... | 2010 | 20517273 |
| molecular analysis of two novel neisseria gonorrhoeae virulent components: the macrophage infectivity potentiator and the outer membrane protein a. | molecular analyses of mip and ompa genes were performed on 20 neisseria gonorrhoeae isolates. the genes were present with a high degree of conservation in all strains. sera from patients with urethritis or disseminated gonococcal infections were able to recognize the purified neisseria gonorrhoeae macrophage infectivity potentiator (ng-mip) and neisseria gonorrhoeae outer membrane protein a (ng-ompa). | 2010 | 20518279 |
| amplification of minute amounts of oral bacterial dna for real-time quantitative pcr analysis. | high-throughput technologies for typing caries or health-associated bacterial populations including pcr, dna microarrays and next-generation sequencing techniques require significant amounts of bacterial dna. in clinical settings, the amount of sampled dna is often limited and amplification is therefore essential. protocols should be able to reproducibly amplify sequences in order to maintain initial sequence ratios and should not bias the representation of particular dna sequence types. | 2010 | 20881394 |
| pneumococcal carriage is more common in asthmatic than in non-asthmatic young men. | the aim was to investigate the prevalence of oropharyngeal carriage of streptococcus pneumoniae, haemophilus influenzae, moraxella catarrhalis, neisseria meningitidis and beta-haemolytic streptococci among asthmatic and non-asthmatic young finnish men and to identify putative risk factors. | 2010 | 20887345 |
| [clinical application of azithromycin extended-release (er) formulation to treat female sexually transmitted infection]. | the prevalence of female sexually transmitted infection (sti) in japan is in the decreasing tendency after 2002, however it still actualizes as a social problem. azithromycin, which is 15-member macrolide antimicrobial agent, has indication to treat the chlamydia sti in a single dose of 1 g. in april 2009, a single dose of 2 g of azithromycin extended release (er) formulation, which is improved formulation by the viewpoint of pharmacokinetics-pharmacodynamics, was approved and has indications to ... | 2010 | 20919495 |
| estimating the number of unlicensed brothels operating in melbourne. | to estimate the number of unlicensed brothels operating in melbourne, australia, and the sexual health of the women working in them. | 2010 | 20920108 |
| immunogenicity and safety of a neugcgm3 based cancer vaccine: results from a controlled study in metastatic breast cancer patients. | increased levels of neugc-containing gangliosides have been described in human breast cancer. a controlled phase ii clinical trial was conducted in patients with metastatic breast cancer to evaluate immunogenicity, safety and to identify evidences of biological activity of a cancer vaccine composed by neugcgm3 in a proteoliposome of neisseria meningitidis together with montanide isa 51 as adjuvant. after first line chemotherapy, 79 women were randomized 1:1 to receive the vaccine candidate or be ... | 2010 | 20855939 |
| neighborhood environment, sexual risk behaviors and acquisition of sexually transmitted infections among adolescents diagnosed with psychological disorders. | the association between neighborhood environment and prevalence of stis, sexual partner variables and condom use among adolescents with psychological disorders was examined. cross-sectional data in three urban areas of the us (southeast, northeast and midwest) were obtained from 384 sexually active male and female participants who provided urine samples for laboratory-confirmed testing of neisseria gonorrhoeae, chlamydia trachomatis and trichomonas vaginalis. a total of 15.4% of participants tes ... | 2010 | 20857329 |
| responses to a conjugate pneumococcal vaccine in preterm infants immunized at 2, 3, and 4 months of age. | preterm infants are at an increased risk of invasive pneumococcal disease infection and, additionally, have a diminished response to haemophilus influenzae type b (hib) conjugate vaccines. there are little data examining the response of preterm infants to a seven-valent pneumococcal conjugate vaccine (pcv7). we examined the responses of preterm infants immunized at 2, 3, and 4 months of age to pcv7. a total of 133 preterm and 54 term infants were immunized with pcv7 and the neisseria meningitidi ... | 2010 | 20861323 |
| neisseria meningitidis rifampicin resistant strains: analysis of protein differentially expressed. | several mutations have been described as responsible for rifampicin resistance in neisseria meningitidis. however, the intriguing question on why these strains are so rare remains open. the aim of this study was to investigate the protein content and to identify differential expression in specific proteins in two rifampicin resistant and one susceptible meningococci using two-dimensional electrophoresis (2-de) combined with mass spectrometry. | 2010 | 20868485 |
| analogies and homologies in lipopolysaccharide and glycoprotein biosynthesis in bacteria. | bacteria generate and attach countless glycan structures to diverse macromolecules. despite this diversity, the mechanisms of glycoconjugate biosynthesis are often surprisingly similar. the focus of this review is on the commonalities between lipopolysaccharide (lps) and glycoprotein assembly pathways and their evolutionary relationship. three steps that are essential for both pathways are completed by membrane proteins. these include the initiation of glycan assembly through the attachment of a ... | 2010 | 20871101 |
| the prevalence of chlamydia trachomatis and neisseria gonorrhoeae in the endocervical swab specimens of symptomatic, asymptomatic and infertile women in turkey. | to investigate c. trachomatis and n. gonorrhoeae prevalence in three different female populations in turkey. | 2010 | 20872148 |
| a duplex pcr method to identify mosaic pena gene and predict reduced susceptibility to oral cephalosporins in neisseria gonorrhoeae. | neisseria gonorrhoeae with mosaic penicillin-binding protein 2 (pbp2) is associated with reduced susceptibility to third-generation oral cephalosporins. a simple and rapid pcr method using three primers was designed to identify mosaic pbp2, which could help predict reduced susceptibility to expanded-spectrum cephalosporins in n. gonorrhoeae. | 2010 | 20801168 |
| human antibody responses to the meningococcal factor h binding protein (lp2086) during invasive disease, colonization and carriage. | recombinant forms of neisseria meningitidis factor h binding protein (fhbp) are undergoing clinical trials in candidate vaccines against serogroup b meningococcal disease. little is known, however, about the host response to fhbp during natural carriage and disease. here we report a longitudinal study of the antibody response to fhbp in healthy meningococcal carriers and non-carriers, and in patients with invasive meningococcal disease. using a highly sensitive quantitative immunoassay, anti-fhb ... | 2010 | 20875489 |
| enhancement of the protective efficacy of a chlamydia trachomatis recombinant vaccine by combining systemic and mucosal routes for immunization. | chlamydia trachomatis causes respiratory and sexually transmitted infections. here, we tested a vaccine formulated with the recombinant major outer membrane protein from c. trachomatis mouse pneumonitis (ct-mopn) for its ability to protect mice against an intranasal (i.n.) challenge. the adjuvants cpg and montanide were used for systemic routes, intramuscular (i.m.) and subcutaneous (s.c.), and cholera toxin for mucosal routes, sublingual (s.l.) and colonic (c.l.). mucosal immunizations were per ... | 2010 | 20875490 |
| haemophilus influenzae type b carriage in indigenous children and children attending childcare centers in the northern territory, australia, spanning pre- and post-vaccine eras. | this paper investigates haemophilus influenzae type b (hib) carriage in indigenous children and children attending childcare centres in the northern territory of australia, 1992-2005. more than 10 years after the introduction of the hib conjugate vaccine, hib carriage persists in indigenous children (3.4%, 2003-2005) and children attending childcare centres (0.2%, 2004). this is the first australian study to examine hib carriage spanning the pre- and post-vaccination eras. increasing carriage ra ... | 2010 | 20875498 |
| meningococcal quadrivalent (serogroups a, c, w135, and y) conjugate vaccine (menveo): in adolescents and adults. | menveo is a quadrivalent meningococcal polysaccharide conjugate vaccine containing the four neisseria meningitidis capsular polysaccharides, a, c, w135, and y, each conjugated to the mutant diphtheria toxin, known as crossreactive material 197 (crm(197)). administration of a single dose of the menveo vaccine elicited a strong immune response against all four vaccine serogroups in adolescents and adults in randomized, single- or multicenter, phase ii or iii trials. in adolescents, menveo was gene ... | 2010 | 20795751 |
| the crystal structure of a bacterial sufu-like protein defines a novel group of bacterial proteins that are similar to the n-terminal domain of human sufu. | sufu (suppressor of fused), a two-domain protein, plays a critical role in regulating hedgehog signaling and is conserved from flies to humans. a few bacterial sufu-like proteins have previously been identified based on sequence similarity to the n-terminal domain of eukaryotic sufu proteins, but none have been structurally or biochemically characterized and their function in bacteria is unknown. we have determined the crystal structure of a more distantly related sufu-like homolog, ngo1391 from ... | 2010 | 20836087 |
| immunogenicity of two investigational serogroup b meningococcal vaccines in the first year of life: a randomized comparative trial. | an investigational vaccine against serogroup b meningococcal (menb) disease containing 3 main recombinant proteins (factor h-binding protein, neisserial adhesion a, and neisserial heparin-binding antigen) has been developed. we evaluated the immunogenicity and reactogenicity of a 3-dose course of this vaccine administered alone (recombinant menb [rmenb]) or combined with the outer membrane vesicle (omv) component of the vaccine used in new zealand (rmenb+omv). | 2010 | 20844462 |
| the bacterial microbiota in the oral mucosa of rural amerindians. | the oral microbiota plays an important role in buccal health and in diseases such as periodontitis and meningitis. the study of the human oral bacteria has so far focused on subjects from western societies, while little is known about subjects from isolated communities. this work determined the composition of the oral mucosa microbiota from six amazon amerindians, and tested a sample preservation alternative to freezing. paired oral swabs were taken from six adults of guahibo ethnicity living in ... | 2010 | 20847007 |
| meningococcal disease: the organism, clinical presentation, and worldwide epidemiology. | 2010 | 20849427 | |
| the risk of meningococcal disease in travelers and current recommendations for prevention. | 2010 | 20849428 | |
| less is more: neisseria gonorrhoeae recx protein stimulates recombination by inhibiting reca. | escherichia coli recx (recx(ec)) is a negative regulator of reca activities both in the bacterial cell and in vitro. in contrast, the neisseria gonorrhoeae recx protein (recx(ng)) enhances all reca-related processes in n. gonorrhoeae. surprisingly, the recx(ng) protein is not a reca protein activator in vitro. instead, recx(ng) is a much more potent inhibitor of all reca(ng) and reca(ec) activities than is the e. coli recx ortholog. a series of recx(ng) mutant proteins representing a gradient of ... | 2010 | 20851893 |
| risk factors associated with chlamydia and gonorrhoea infection among female sex workers in two mexico-usa border cities. | female sex workers (fsws) aged ≥18 years without known hiv infection living in tijuana and ciudad juarez, mexico who had recent unprotected sex with clients underwent interviews and testing for chlamydia and gonorrhoea using nucleic acid amplification. correlates of each infection were identified with logistic regression. among 798 fsws, prevalence of chlamydia and gonorrhoea was 13.0% and 6.4%, respectively. factors independently associated with chlamydia were younger age, working in tijuana ve ... | 2010 | 20852194 |
| aetiological agents of urethritis in symptomatic south african men attending a family practice. | the objective was to determine the occurrence of four urethral pathogens in urine specimens from symptomatic men using transcription mediated amplification (tma) assay. urethral swab and urine specimens from 300 men presenting to a family practitioner were gram stained and tested for neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis and mycoplasma genitalium using three tma assays respectively. except for t. vaginalis, the other recognized pathogens viz. n. gonorrhoeae, c. trac ... | 2010 | 20852197 |
| the pathogenesis of infection and immune response. | an understanding of the pathogenesis of infection, and the complex and fascinating 'relationship', or interaction, between the infecting organism and the host are advantageous when caring for patients with infections/infectious diseases, particularly as many of the clinical features of infection are as a consequence of the defence mechanisms mounted by the immune system. this article aims to provide a brief overview of the pathogenesis of infection, explaining how the two branches of the immune ... | 2010 | 20852457 |
| human skin allografts as a useful adjunct in the treatment of purpura fulminans. | the aim of this report is to discuss the role of human skin allografts in surgical coverage procedures for patients with purpura fulminans. | 2010 | 20852508 |
| the fundamental nature of meningococcal disease. | 2010 | 20472222 | |
| electrochemical dna sensor for neisseria meningitidis detection. | meningitis sensor based on nucleic acid probe of neisseria meningitidis has been fabricated by immobilization of 5'-thiol end labeled single stranded deoxyribonucleic acid probe (ssdna-sh) onto gold (au) coated glass electrode. this ssdna-sh/au electrode hybridized with the genomic dna (g-dsdna/au) and amplified dna (pcr-dsdna/au) has been characterized using atomic force microscopy (afm), fourier transforms infrared spectroscopy (ft-ir) and electrochemical techniques. the ssdna-sh/au electrode ... | 2010 | 20472423 |
| frequency and rate of pilin antigenic variation of neisseria meningitidis. | the rates of pilin antigenic variation (av) of two strains of neisseria meningitidis were determined using an unbiased dna sequencing assay. strain mc58 underwent pilin av at a rate similar to that of n. gonorrhoeae strain ms11 but lower than that of n. gonorrhoeae strain fa1090. pilin av was undetectable in strain fam18. | 2010 | 20472803 |
| the elephant never forgets; piloting a chlamydia and gonorrhea retesting reminder postcard in an std clinic setting. | we examined the number and proportion of persons retesting and reinfected with chlamydia and/or gonorrhea 3 to 4 months after initial infection in one new york city sexually transmitted disease clinic using a reminder postcard compared to clinics not using this reminder. retesting increased; however, the proportion reinfected was lower during the intervention. | 2010 | 20473247 |
| pelvic inflammatory disease. | pelvic inflammatory disease (pid) is an infection-caused inflammatory continuum from the cervix to the peritoneal cavity. most importantly, it is associated with fallopian tube inflammation, which can lead to infertility, ectopic pregnancy, and chronic pelvic pain. the microbial etiology is linked to sexually transmitted microorganisms, including chlamydia trachomatis, neisseria gonorrheae, mycoplasma genitalium, and bacterial vaginosis-associated microorganisms, predominantly anaerobes. pelvic ... | 2010 | 20664404 |
| neisseria meningitidis serogroup x sequence type 767 in turkey. | 2010 | 20739496 | |
| subgingival microbial profiles of smokers with periodontitis. | the subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. we tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16s sequencing. smokers ... | 2010 | 20739702 |
| comprehensive assessment of sociodemographic and behavioral risk factors for mycoplasma genitalium infection in women. | neisseria gonorrhoeae and chlamydia trachomatis are characterized by different risk factors, thus control strategies for each also differ. in contrast, risk factors for mycoplasma genitalium have not been well characterized. | 2010 | 20679963 |
| design and evaluation in mice of a broadly protective meningococcal group b native outer membrane vesicle vaccine. | a vaccine based on native outer membrane vesicles (nomv) that has potential to provide safe, broad based protection against group b strains of neisseria meningitidis has been developed. three antigenically diverse group b strains of n. meningitidis were chosen and genetically modified to improve safety and expression of desirable antigens. safety was enhanced by disabling three genes: synx, lpxl1, and lgta. the vaccine strains were genetically configured to have three sets of antigens each with ... | 2010 | 20685194 |
| genome-based vaccine development: a short cut for the future. | bacterial infectious diseases remain a major cause of deaths and disabilities in the world. although conventional vaccinology approaches were successful in conferring protection against several diseases, they failed in providing efficient vaccines against many others. together to the sequencing of the first genome, a new chapter in the vaccinology history started to be written. reverse vaccinology changed the way to think about vaccine development, using the information provided by the microorga ... | 2010 | 20686357 |
| the meningococcal vaccine candidate neisserial surface protein a (nspa) binds to factor h and enhances meningococcal resistance to complement. | complement forms an important arm of innate immunity against invasive meningococcal infections. binding of the alternative complement pathway inhibitor factor h (fh) to fh-binding protein (fhbp) is one mechanism meningococci employ to limit complement activation on the bacterial surface. fhbp is a leading vaccine candidate against group b neisseria meningitidis. novel mechanisms that meningococci employ to bind fh could undermine the efficacy of fhbp-based vaccines. we observed that fhbp deletio ... | 2010 | 20686663 |
| invasive neisseria meningitidis with decreased susceptibility to fluoroquinolones in south africa, 2009. | 2010 | 20688769 | |
| annual report of the australian gonococcal surveillance programme, 2009. | the australian gonococcal surveillance programme (agsp) monitors antibiotic susceptibility testing of neisseria gonorrhoeae isolated in all states and territories. in 2009 the in vitro susceptibility of 3,220 isolates of gonococci from public and private sector sources was determined by standardised methods. varying antibiotic susceptibility patterns were again seen across jurisdictions and regions. resistance to the penicillins nationally was 36% and, with the exception of the northern territor ... | 2010 | 20690208 |
| cephalosporin mic creep among gonococci: time for a pharmacodynamic rethink? | gonorrhoea has been among the easiest infections to cure with antibiotics. nevertheless, emerging resistance has driven repeated treatment shifts. decreased cephalosporin susceptibility is now being reported. we examined cephalosporin mic trends for neisseria gonorrhoeae in the uk and undertook pharmacodynamic analyses to predict efficacy against strains with raised mics. | 2010 | 20693173 |
| genome-wide association study identifies variants in the cfh region associated with host susceptibility to meningococcal disease. | meningococcal disease is an infection caused by neisseria meningitidis. genetic factors contribute to host susceptibility and progression to disease, but the genes responsible for disease development are largely unknown. we report here a genome-wide association study for host susceptibility to meningococcal disease using 475 individuals with meningococcal disease (cases) and 4,703 population controls from the uk. we performed, in western european and south european cohorts (consisting of 968 cas ... | 2010 | 20694013 |
| combined haemophilus influenzae type b and neisseria meningitidis serogroup c (hibmenc) or serogroup c and y-tetanus toxoid conjugate (and hibmency) vaccines are well-tolerated and immunogenic when administered according to the 2,3,4 months schedule with a fourth dose at 12-18 months of age. | combined hibmency and hibmenc conjugate vaccines may facilitate inclusion of vaccination against menc and meny into routine vaccination schedules, without additional injections. immunogenicity and reactogenicity of vaccination with three different formulations of a novel hibmency-conjugate vaccine, or a hibmenc-conjugate vaccine was assessed. infants were randomized to receive either hib(2.5 µg)-menc(5 µg)-meny(5 µg)-tt, hib(5 µg)-menc(10 µg)-meny(10 µg)-tt, hib(5 µg)-menc(5 µg)-meny(5 µg)-tt or ... | 2010 | 20697200 |
| pediatric vaccines on the horizon. | vaccines have saved the lives of millions of children and continue to be essential interventions to control infectious diseases among people of all ages. the list of recommended vaccines for children has expanded in recent years; however, many viral, bacterial and parasitic infections remain a major cause of morbidity and mortality in children. improved vaccines to prevent streptococcus pneumoniae and neisseria meningitidis infections in children will soon be available. recent scientific advance ... | 2010 | 20697256 |
| a comparison of meningococcal carriage by pregnancy status. | neisseria meningitidis is the second leading cause of invasive meningitis. a prerequisite for infection is colonization of the nasopharynx, and asymptomatic carrier rates are widely reported in the range of 10-15%. recent reports have indicated an increased likelihood that a pediatric admission for neisseria meningitidis will have a mother who is pregnant in the home. we hypothesized that this association may relate to immunologic changes in pregnancy leading to higher carrier rates.we compared ... | 2010 | 20701795 |
| molecular and structural analysis of mosaic variants of penicillin-binding protein 2 conferring decreased susceptibility to expanded-spectrum cephalosporins in neisseria gonorrhoeae: role of epistatic mutations. | mutations in penicillin-binding protein 2 (pbp 2) encoded by mosaic pena alleles are crucial for intermediate resistance to the expanded-spectrum cephalosporins ceftriaxone and cefixime in neisseria gonorrhoeae. three of the ∼60 mutations present in mosaic alleles of pena, g545s, i312m, and v316t, have been reported to be responsible for increased resistance, especially to cefixime [takahata, s., et al. (2006) antimicrob. agents chemother. 50, 3638-3645]. however, we observed that the minimum in ... | 2010 | 20704258 |
| prevalence and risk factors of sexually transmitted infections and cervical neoplasia in women from a rural area of southern mozambique. | there is limited information on the prevalence of sexually transmitted infections and the prevalence of cervical neoplasia in rural sub-saharan africa. this study describes the prevalence and the etiology of stis and the prevalence of cervical neoplasia among women in southern mozambique. an age-stratified cross-sectional study was performed where 262 women aged 14 to 61 years were recruited at the antenatal clinic (59%), the family-planning clinic (7%), and from the community (34%). at least on ... | 2010 | 20706691 |
| epidemiological profile of acute bacterial meningitis in the state of rio grande do norte, brazil. | acute bacterial meningitis (abm) remains a public health problem in brazil. to evaluate the epidemiology of abm cases at giselda trigueiro hospital, rio grande do norte, a descriptive retrospective survey was conducted covering 2005 to 2008. | 2010 | 20802949 |
| transplacental transport of igg antibodies specific for pertussis, diphtheria, tetanus, haemophilus influenzae type b, and neisseria meningitidis serogroup c is lower in preterm compared with term infants. | maternal antibodies, transported through the placenta during pregnancy, contribute to the protection of infants from infectious diseases during the first months of life. the aim of this study was to measure the concentration of antibodies against several vaccine-preventable diseases in paired maternal and cord blood serum samples in preterm and term infants and to assess placental transfer ratios and infant antibody concentrations against vaccine-preventable diseases. | 2010 | 20803841 |
| aptima assay on surepath liquid-based cervical samples compared to endocervical swab samples facilitated by a real time database. | liquid-based cytology (lbc) cervical samples are increasingly being used to test for pathogens, including: hpv, chlamydia trachomatis (ct) and neisseria gonorrhoeae (gc) using nucleic acid amplification tests. several reports have shown the accuracy of such testing on thinprep (tp) lbc samples. fewer studies have evaluated surepath (sp) lbc samples, which utilize a different specimen preservative. this study was undertaken to assess the performance of the aptima combo 2 assay (ac2) for ct and gc ... | 2010 | 20806073 |
| vitronectin in bacterial pathogenesis: a host protein used in complement escape and cellular invasion. | the multifunctional human glycoprotein vitronectin (vn) plays a significant role in cell migration, tissue repair and regulation of membrane attack complex (mac) formation. it also promotes neutrophil infiltration and, thus, enhances the inflammatory process during infection. in the host, a balanced homeostasis is maintained by vn due to neutralization of the self-reactivity of the mac. on the other hand, vn bound to the bacterial surface protects from mac-mediated lysis and enhances adhesion. g ... | 2010 | 20807208 |
| outbreak of invasive meningococcal disease in goleniow county, northwest poland, march 2009. | 2010 | 20807486 | |
| tyrosine-phosphorylated caveolin-1 blocks bacterial uptake by inducing vav2-rhoa-mediated cytoskeletal rearrangements. | certain bacterial adhesins appear to promote a pathogen's extracellular lifestyle rather than its entry into host cells. however, little is known about the stimuli elicited upon such pathogen host-cell interactions. here, we report that type iv pili (tfp)-producing neisseria gonorrhoeae (p(+)gc) induces an immediate recruitment of caveolin-1 (cav1) in the host cell, which subsequently prevents bacterial internalization by triggering cytoskeletal rearrangements via downstream phosphotyrosine sign ... | 2010 | 20808760 |
| structural alterations in a component of cytochrome c oxidase and molecular evolution of pathogenic neisseria in humans. | three closely related bacterial species within the genus neisseria are of importance to human disease and health. neisseria meningitidis is a major cause of meningitis, while neisseria gonorrhoeae is the agent of the sexually transmitted disease gonorrhea and neisseria lactamica is a common, harmless commensal of children. comparative genomics have yet to yield clear insights into which factors dictate the unique host-parasite relationships exhibited by each since, as a group, they display remar ... | 2010 | 20808844 |
| ophthalmia neonatorum. | to measure the frequency of ophthalmia neonatorum among all babies born in mch center, pims (pakistan institute of medical sciences), islamabad, in one month's time and to determine their causative organisms. | 2010 | 20810051 |
| human-restricted bacterial pathogens block shedding of epithelial cells by stimulating integrin activation. | colonization of mucosal surfaces is the key initial step in most bacterial infections. one mechanism protecting the mucosa is the rapid shedding of epithelial cells, also termed exfoliation, but it is unclear how pathogens counteract this process. we found that carcinoembryonic antigen (cea)-binding bacteria colonized the urogenital tract of cea transgenic mice, but not of wild-type mice, by suppressing exfoliation of mucosal cells. cea binding triggered de novo expression of the transforming gr ... | 2010 | 20813953 |
| periurethral abscess complicating gonococcal urethritis: case report and literature review. | 2010 | 20814714 | |
| variation in the neisseria meningitidis fadl-like protein: an evolutionary model for a relatively low-abundance surface antigen. | the molecular diversity of a novel neisseria meningitidis antigen, encoded by the orf nmb0088 of mc58 (fadl-like protein), was assessed in a panel of 64 diverse meningococcal strains. the panel consisted of strains belonging to different serogroups, serotypes, serosubtypes and mlst sequence types, of different clinical sources, years and countries of isolation. based on the sequence variability of the protein, the fadl-like protein has been divided into four variant groups in this species. antig ... | 2010 | 20817647 |
| crystallization and preliminary crystallographic characterization of the iron-regulated outer membrane lipoprotein frpd from neisseria meningitidis. | fe-regulated protein d (frpd) is a neisseria meningitidis outer membrane lipoprotein that may be involved in the anchoring of the secreted repeat in toxins (rtx) protein frpc to the outer bacterial membrane. however, the function and biological roles of the frpd and frpc proteins remain unknown. native and selenomethionine-substituted variants of recombinant frpd43-271 protein were crystallized using the sitting-drop vapour-diffusion method. diffraction data were collected to a resolution of 2.2 ... | 2010 | 20823542 |
| matrix-assisted laser desorption ionization time-of-flight mass spectrometry: usefulness for taxonomy and epidemiology. | matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry (ms) is a powerful tool for the species and subspecies classification of a broad spectrum of bacteria, including gram-positive bacteria such as staphylococcus, streptococcus and listeria, and gram-negative bacteria such as neisseria, salmonella, aeromonas, campylobacter and helicobacter. maldi-tof ms has also been used for the rapid identification and typing of potential bioterrorism agents, including coxiel ... | 2010 | 20825435 |
| bacterial 16s sequence analysis of severe caries in young permanent teeth. | previous studies have confirmed the association of the acid producers streptococcus mutans and lactobacillus spp. with childhood caries, but they also suggested these microorganisms are not sufficient to explain all cases of caries. in addition, health-associated bacterial community profiles are not well understood, including the importance of base production and acid catabolism in ph homeostasis. the bacterial community composition in health and in severe caries of the young permanent dentition ... | 2010 | 20826648 |
| factor h facilitates adherence of neisseria gonorrhoeae to complement receptor 3 on eukaryotic cells. | neisseria gonorrhoeae can engage human complement receptor 3 (cr3) directly or through surface-bound ic3b. factor h (fh) that binds to bacteria facilitates conversion of c3b to ic3b. fh also binds directly to cr3 on professional phagocytes. certain nonprofessional phagocytes, such as primary cervical epithelial cells, also express cr3. we hypothesized that fh could bridge bacteria to cr3 and facilitate gonococcal association with host cells. specificity of the fh-cr3 interaction was confirmed us ... | 2010 | 20826755 |
| immune inhibitory ligand cd200 induction by tlrs and nlrs limits macrophage activation to protect the host from meningococcal septicemia. | macrophage activation is essential for protection against bacterial pathogens but needs to be regulated to prevent damage to the host. we show a key role for the immune inhibitory receptor cd200r and its ligand cd200 in the context of infection with the gram-negative human pathogen neisseria meningitidis. n. meningitidis induced cd200 but downregulated cd200r on macrophages in a manner dependent on neisserial lipopolysaccharide, toll-like receptor-4 (tlr-4), and the myd88 pathway but independent ... | 2010 | 20833375 |
| functional analysis of the carbohydrate-binding module of an esterase from neisseria sicca sb involved in the degradation of cellulose acetate. | an esterase gene from neisseria sicca sb encoding caea, which catalyzes the deacetylation of cellulose acetate, was cloned. caea contained a putative catalytic domain of carbohydrate esterase family 1 and a carbohydrate-binding module (cbm) family 2. we constructed two derivatives, with and without the cbm of caea. binding assay indicated that the cbm of caea had an affinity for cellulose. | 2010 | 20834142 |
| microbiological epidemiological history of meningococcal disease in rio de janeiro, brazil. | the main objectives of the present study were to investigate the clinical and laboratory features of meningococcal disease in the city of rio de janeiro, brazil, during the overlap of 2 epidemics in the 1990s. we conducted a study of a series of cases of meningococcal disease admitted in a meningitis reference hospital. all clinical isolates available were analyzed by means of microbiological epidemiological markers. in 1990, neisseria meningitidis serogroup b:4,7:p1.19,15, 1.7,1 sulfadiazine-re ... | 2010 | 20835507 |
| successful treatment of uncomplicated gonococcal urethritis in hiv-infected patients with single-dose oral cefpodoxime. | fluoroquinolones are no longer recommended for the treatment of gonococcal infections in the united states. cephalosporins - ceftriaxone and cefixime - are the treatment of choice, as suggested by the centers for disease control and prevention (usa). there are limited data on the efficacy of cefpodoxime for the treatment of uncomplicated gonococcal infections. two cases of hiv-infected homosexual men who were successfully treated with cefpodoxime for urethritis caused by neisseria gonorrhoeae ar ... | 2010 | 21886642 |
| meningococcal purpura fulminans in children. ii: late orthopedic sequelae management. | purpura fulminans is a rare and extremely severe infection, mostly due to neisseria meningitidis. nineteen patients were followed up immediately after the initial multivisceral failure in order to diagnose late-onset orthopedic sequelae. we report our experience with these 19 patients, in light of our medical follow-up protocol and surgical management. | 2010 | 21966304 |
| Innate immune mediator profiles and their regulation in a novel polarized immortalized epithelial cell model derived from human endocervix. | The endocervix in the female reproductive tract (FRT) is susceptible to sexually transmitted pathogens such as Chlamydia trachomatis and Neisseria gonorrhoeae. Endocervical epithelial cells in vivo make innate immune mediators that likely aid in the protection from these pathogens. In vitro studies to investigate the innate epithelial cell immune response to endocervical pathogens have been hindered by the paucity of human endocervix-derived epithelial cell lines that display the differentiation ... | 2011 | 21943934 |