Publications

[myocarditis caused by toxoplasma gondii and aspergillus fumigatus after orthotopic heart transplantation].	this report describes the case of a 56-year-old patient, who died 53 days after orthotopic heart transplantation due to myocarditis caused by infection with aspergillus fumigatus and toxoplasma gondii. aspergillosis was diagnosed by transthoracic needle aspiration of a pulmonary infiltration. endomyocardial biopsy showed toxoplasma pseudocysts. autopsy revealed myocardial infection with both infectious agents. despite specific therapy, myocarditis determined the fatal outcome in this case. the v ...	1991	1872010
acute disseminated aspergillosis in a cow with special reference to penetration and spread.	acute disseminated aspergillosis caused by aspergillus fumigatus is reported in a 4-year-old cow given broad spectrum antibiotics and anti-inflammatory therapy. all stomach compartments were infected and hyphal invasion of gastric blood vessels was the probable source of generalization to the liver, lung and kidneys. throughout the peyer's patches of the jejunum there were acute necrotic foci with aspergillus hyphae and similar foci occurred in the corresponding mesenteric lymph nodes. this poin ...	1991	1874983
antimicrobial contact activity of econazole sulfosalicylate.	the aim of this investigation was to compare the contact action of econazole sulfosalicylate (e-ssa) on mycetes (candida albicans, cryptococcus neoformans, aspergillus fumigatus, trichophyton rubrum, t. cutaneum, pityrosporum sp.), gram-positive bacteria (staphylococcus aureus, streptococcus faecalis) and gram-negative bacteria (escherichia coli, citrobacter freundii) with that exerted by econazole nitrate (e-nit). the results show e-ssa activity greater than e-nit (in particular against mycetes ...	1991	1875229
effect of diet and population density on male turkeys under various environmental conditions. 1. turkey growth and health performance.	the performance of 1,312 male market turkeys (large white, nicholas strain) from 0 to 20 wk of age fed diets varying in feed form and energy level was measured under two stocking densities (.21 or .46 m2 per bird) and four lighting and temperature programs. the four diets were 1) corn and soybean meal with 1% supplemental fat, mash (csm); 2) as 1, pelleted (csp); 3) as diet 1 but with 1, 2, 4, 6, and 8% supplemental fat during 0 to 4, 4 to 8, 8 to 12, 12 to 16, and 16 to 20 wk of age, respective ...	1991	1876567
immunolocalization of aspergillus fumigatus mycelial antigens.	specific immunoglobulin from the sera of patients with antibodies to aspergillus and from antisera raised in rabbits to aspergillus fumigatus fractions bound almost exclusively to the mycelial wall, as shown by immunogold labelling of ultra-thin sections. different layers of the wall were labelled, depending on the source of the antigen used to produce antibody. internal, cytoplasmic components were generally not labelled, except with antisera raised to crude wall material and to a concanavalin ...	1991	1880682
the relationship between atopy and cystic fibrosis.	the incidence and clinical significance of allergy in cystic fibrosis have been discussed. there is little evidence that the high prevalence of positive allergy skin tests in cf is a clinical manifestation of a hypersensitivity lung disease complicating the primary pulmonary disorder, except in the special case of allergic bronchopulmonary aspergillosis. the lung disease of cf appears to be caused by excessive bronchial secretions and recurrent infection that are the result of abnormal ion trans ...	1991	1884327
model of recurrent pulmonary aspergillosis in rats.	male sprague-dawley rats were treated with cortisone acetate and fed a low-protein diet for 3 weeks. at the end of week 2, animals were infected intratracheally with 10(5) conidia of aspergillus fumigatus h11-20. despite discontinuation of steroids and the low-protein diet 1 week after the infection, 94% of controls died of invasive pulmonary aspergillosis within 3 weeks postinfection. when rats were treated with a single dose of 1.6 mg of aerosolized amphotericin b per kg of body weight 48 h pr ...	1991	1885728
fatal pulmonary aspergillosis presenting as acute eosinophilic pneumonia in a previously healthy child.	a previously healthy boy presented with cough and diffuse pulmonary interstitial infiltrates. acute eosinophilic pneumonia was diagnosed by bronchoalveolar lavage in the absence of a demonstrable infectious etiologic agent. corticosteroid therapy resulted in immediate improvement but was followed by respiratory distress and death from invasive aspergillosis and pseudomonas cepacia sepsis.	1991	1889295
variability in parameters of allergic bronchopulmonary aspergillosis in patients with cystic fibrosis.	seventy-nine patients with cystic fibrosis (cf) were evaluated and were followed in a longitudinal, prospective fashion during a 6-year period for the development of immune parameters indicating aspergillus fumigatus (af) sensitization and allergic bronchopulmonary aspergillosis (abpa). although four patients developed frank abpa, there was considerable variability in immune parameters in non-abpa. twenty-four patients became skin test positive to af with none losing skin reactivity. twenty-five ...	1991	1890268
experimental candidosis in pregnant mice.	pregnant mice were challenged intravenously with doses of 1 x 10(3)-1 x 10(7) candida albicans blastospores, and from postmortem histopathology it was found that c. albicans had a propensity for localization in the murine placenta. at the highest dose, blastospores, hyphae and pseudohyphae were randomly located in the foeto-placental units, whereas proliferation of fungi at lower doses started in the rim of the placental disc, after which it spread along reichert's membrane and/or the splanchnop ...	1991	1892636
disinfecting activities of non-peroxide soft contact lens cold disinfection solutions.	the antimicrobial activities of three non-peroxide soft contact lens chemical disinfection systems--renu multi-purpose solution (0.00005% polyaminopropyl biguanide), opti-soft disinfecting solution (0.001% polyquaternium-1), and opti-free rinsing, disinfecting & storage solution (0.001% polyquaternium-1)--were compared to soft mate disinfecting solution (0.005% chlorhexidine digluconate). each product was separately inoculated with each of five microorganisms at approximately 10(6) microorganism ...	1991	1893538
[two cases of hypersensitivity pneumonitis due to contamination of an ultrasonic humidifier].	two cases of hypersensitivity pneumonitis due to contamination of ultrasonic-humidifier were reported. the first case, a 64-year-old man, developed fever and dyspnea on exertion in january 1986. he was hospitalized for 18 days and received antibiotics for presumptive bacterial pneumonia. half a day after discharge, those symptoms recurred. on readmission, fine crackles were heard at the left lung base, and chest x-ray film showed ground glass shadows all over the lung fields. bronchoalveolar lav ...	1991	1895586
cystic fibrosis: infection.	recurrent and chronic pulmonary infection is still the major cause of morbidity and mortality in cystic fibrosis. although respiratory viruses are responsible for some of the acute exacerbations of the pulmonary disease, bacteria, and in some patients aspergillus fumigatus, are the most important pathogens. staphylococcus aureus and haemophilus influenzae are the most prevalent pathogens in cystic fibrosis of childhood, whereas pseudomonas aeruginosa and in some centres also pseudomonas cepacia ...	1991	1900640
microbiology of airway disease in patients with cystic fibrosis.	individuals with cystic fibrosis have abbreviated life spans primarily due to chronic airway infection. a limited number of types of organisms are responsible for these infections, with staphylococcus aureus and pseudomonas aeruginosa being of primary importance. in the pre-antibiotic era, greater than 90% of deaths due to infection were caused by s. aureus and death usually occurred in the first 2 years of life. with the advent of effective antistaphylococcal therapy, life spans increased and p ...	1991	1900735
in vivo interferon-gamma therapy augments the in vitro ability of chronic granulomatous disease neutrophils to damage aspergillus hyphae.	during the recently completed double-blind, placebo-controlled, randomized trial of recombinant interferon-gamma (rifn-gamma) therapy in chronic granulomatous disease (cgd), a metabolic assay of neutrophil damage to aspergillus fumigatus hyphae was used to monitor neutrophil function before and during therapy. in this assay, 5 x 10(4) conidia that had germinated into hyphae were exposed to 5 x 10(5), 15 x 10(5), or 50 x 10(5) cgd neutrophils. by analysis of variance, neutrophils from patients on ...	1991	1901332
endoscopic visualization of aspergilloma.		1991	1907396
use of alpha-aminoadipate and lysine as sole nitrogen source by schizosaccharomyces pombe and selected pathogenic fungi.	alpha-aminodipate, an intermediate of the lysine biosynthetic pathway of fungi, or lysine when used as the sole nitrogen source in the medium was growth inhibitory and toxic to saccharomyces cerevisiae. the fission yeast schizosaccharomyces pombe and pathogenic fungi candida albicans, filobasidiella neoformans and aspergillus fumigatus grew in the medium containing alpha-aminoadipate as the sole nitrogen source. c. albicans, a. fumigatus, and one of the strains of f. neoformans also grew in the ...	1991	1908900
isolation, amino acid sequence, and synthesis of dermaseptin, a novel antimicrobial peptide of amphibian skin.	a 34-residue antimicrobial peptide named dermaseptin was purified to homogeneity from amphibian skin by a 3-step protocol involving molecular sieve filtration, ion-exchange chromatography, and reversed-phase high-performance liquid chromatography. the complete amino acid sequence of dermaseptin, alwktmlkklgtmalhagkaalgaaadtisqgtq, was determined by automated edman degradation of the peptide and of fragments generated by trypsin. fast atom bombardment mass spectra of dermaseptin gave a protonated ...	1991	1909573
combined surgical and medical treatment of fungal abscess in a pseudophakic eye.		1991	1911514
allergic bronchopulmonary aspergillosis among asthmatics.	allergic bronchopulmonary aspergillosis (abpa), an immunologically mediated lung disease is now well recognised as a significant cause of chronic lung disease in the west. this study aimed at determining the extent of this entity in 540 patients with severe bronchial asthma and 96 patients with mild or moderate asthma. of these 134 were subjected to detailed examinations in the form of a skin test, serological test, sputum examination and peripheral blood eosinophil count. eight patients (1.2% o ...	1991	1913984
[immunologic response to microorganisms from a polluted ventilation system in a working environment].	authors point out the morbidity of employees working in ventilation systems contaminated with various microorganisms. they analysed 96 workers exposed to air conditioning system (group a), and 71 workers (group b) breathing normal ambient air. the workers of both groups were subjected clinically by functionally and immunologically. preparation of antigens "mmm" (monday morning miseries) was used as an original method by ajello et al. for producing antigens from systemic mycotic agents and subseq ...	1991	1916435
characterization of a monoclonal antibody against concanavalin a binding antigen of aspergillus fumigatus.	a monoclonal antibody was produced against a concanavalin a (con a) binding major epitope of aspergillus fumigatus using a novel method of immunization. the antigen was purified using monoclonal antibody affinity chromatography reacted with specific antibodies present in human sera. both allergic bronchopulmonary aspergillosis and aspergilloma showed high levels of antibody, against this purified antigen, when compared to normal controls. similar results were obtained when the monoclonal antibod ...	1991	1916849
[a case of pulmonary aspergillosis with a tumor shadow, diagnosed by transbronchial aspiration cytology].	a 41-year-old female was admitted because of cough and hemosputum. she had no underlying disease and the presence of respiratory disease had not been pointed out. chest x-ray and ct scan showed a solid, homogeneous tumor with a distinct margin near the right hilum. the tumor measured approximately 5 x 4 cm. because other laboratory data concerning the serum levels of tumor marker and anti-aspergillus antibody were normal, benign lung tumor or malignant lymphoma was suspected at first. transbronc ...	1991	1920992
aspergillosis of the cns in a pediatric liver transplant recipient: case report and review.	a 2-month-old infant who had undergone orthotopic liver transplantation at the age of 2 weeks for carbamoyl phosphate synthetase deficiency developed infection of the cns due to aspergillus fumigatus. the patient was successfully treated with administration of a combination of antifungal agents (including intraventricular amphotericin b), drainage of the parietal lobe abscess, and cessation of immunosuppression. an intraventricular catheter was used both to obtain ventricular fluid for microbiol ...	1991	1925287
aspergillus osteomyelitis of the spine.	aspergillosis involving either the vertebral body or the intervertebral disc is a rare cause of osteomyelitis of the spine. the following is a report of five cases of aspergillus fumigatus infection of the spine treated successfully with amphotericin b and 5-flucytosine. in three patients, the diagnosis was established at closed-needle biopsy; two patients with paraplegia had an anterior decompression and fusion. the follow-up period ranged from 19 to 48 months.	1991	1925749
aspergillus fumigatus specific ige and igg antibodies for diagnosis of aspergillus-related lung diseases.	ige and igg antibodies against aspergillus fumigatus were detected by crossed radio immunoelectrophoresis (crie) on the sera of seven patients with aspergilloma, six patients with allergic broncho-pulmonary aspergillosis (abpa) and 25 patients with extrinsic asthma with aspergillus allergy. ige-crie analysis indicated the presence of a. fumigatus-specific ige in sera of patients with abpa and aspergillus asthma but not of aspergilloma patients. igg-crie showed that both aspergilloma and abpa pat ...	1991	1928661
efficacy of cilofungin alone and in combination with amphotericin b in a murine model of disseminated aspergillosis.	cilofungin, amphotericin b, and a combination of the two drugs were compared in a model of aspergillosis in immunocompetent mice in three experiments. cilofungin was equivalent to amphotericin b in preventing death and eradicating cerebral aspergillosis, but it did not sterilize the kidneys. this is the first demonstration of the in vivo activity of cilofungin against any fungus other than candida albicans. the mortality with combination therapy was higher than those with amphotericin b alone (p ...	1991	1929289
fungal endophthalmitis. an experimental study with a review of 17 human ocular cases.	pseudallescheria boydii is an opportunistic fungus that is histologically indistinguishable from aspergillus fumigatus. pseudallescheria boydii has been reported to cause endophthalmitis, orbital cellulitis, and corneal ulceration and is, thus, important to the ophthalmologist. a clinical review of 17 patients with p boydii ophthalmic infections is presented. in addition, animal models of endophthalmitides caused by a fumigatus and p boydii were created and compared. dutch-belted rabbits used fo ...	1991	1929959
novel broad-spectrum metal-based antifungal agents. correlations amongst the structural and biological properties of copper (ii) 2-acetylpyridine n4-dialkylthiosemicarbazones.	copper(ii) complexes of the type [cu(l)x], where l = tridentate anion of 2-acetylpyridine n4-diethyl thiosemicarbazone and x = c1 or br, were screened against seven fungal strains pathogenic to man viz. aspergillus niger, aspergillus fumigatus, candida albicans, cryptococcus neoformans, tricophyton rubrum, epidermophyton floccosum and microsporum canis. the greater growth inhibition exhibited by the bromo complex can be explained on the basis of its lower cu(ii)/cu(i) redox couple and greater co ...	1991	1931432
[therapy of systemic mycoses in immunodeficiency].	fungal infections have gained importance recently. the major reason for this is the increasing number of patients with immunodeficiency. systemic treatment of invasive fungal infections up to now has been based on relatively few antimycotic agents (amphotericin b, flucytosine, as well as the azole derivatives fluconazole and itraconazole). only a few number of fungi cause the majority of opportunistic fungal infections. candida albicans leads to severe mucosal infections in cases of immunodefici ...	1991	1937558
developments in the serological diagnosis of opportunistic fungal infections.	recent years have seen some progress in the design of serodiagnostic tests for the medically important invasive mycoses. this has included the introduction of latex agglutination tests for antigen detection in systemic candidosis and invasive aspergillosis. the circulating cytoplasmic antigens of candida albicans and aspergillus fumigatus have recently been delineated as heat-shock proteins of approximate molecular weight 90 kd.	1991	1938704
heat shock protein 88 and aspergillus infection.	immunoblotting was used to dissect the antibody responses in the sera of 50 patients with proven invasive aspergillosis, 28 patients with suspected invasive aspergillosis, 35 patients with allergic bronchopulmonary aspergillosis, and 10 patients with an aspergilloma. this demonstrated the immunodominance of antigenic bands at 88, 84, 51, and 40 kda. monoclonal antibodies against the heat shock protein 90 complexes of candida albicans and the water mold achlya ambisexualis identified these four a ...	1991	1939562
antigen detection in invasive aspergillosis.	the application of a reverse passive latex agglutination test and dot-blot assay are reported in the diagnosis of 50 proven cases of invasive aspergillosis and 28 suspected cases. at a latex titre cut off of greater than or equal to 1 in 8 the test had a sensitivity of 29.4%, specificity of 96.3% and efficiency of 41.4%. this was in sera taken when the diagnosis was first suggested. the sensitivity rose to 55.1% when sera with the maximum level of antigen were examined. the dot blot was more sen ...	1991	1940388
endemic and epidemic aspergillosis associated with in-hospital replication of aspergillus organisms.	when a new hospital opened in 1983, environmental culturing for aspergillus organisms and surveillance for nosocomial aspergillosis cases were begun to characterize the relationship between environmental contamination and infection. monthly air sampling demonstrated increasing concentrations of aspergillus flavus and aspergillus fumigatus to mean levels greater than 1 cfu/m3 during 1986-1987, accompanied by a progressive increase in incidence of aspergillosis to 1.2% in immunocompromised patient ...	1991	1940482
secretion of a potential virulence factor, a fungal ribonucleotoxin, during human aspergillosis infections.	we show by cloning and nucleotide sequence analysis that the 18kda antigen found in the urine of patients suffering from aspergillosis is related to the fungal protein toxins restrictocin and mitogillin. these are inhibitors of translation which act by catalytic inactivation of eukaryotic ribosomes; they may be implicated in the pathogenesis of the disease.	1991	1943712
native pulmonary sepsis after single-lung transplantation.		1991	1949183
kinetics of transfused neutrophils in peripheral blood and bal fluid of a patient with variant x-linked chronic granulomatous disease.	patients with chronic granulomatous disease (cgd), an uncommon inherited disorder of phagocytes resulting in the defective production of reactive oxygen intermediates, are prone to bacterial and fungal infections. in the case presented, therapeutic efforts including white cell transfusions, and amphotericin b and ifn gamma administration were undertaken to treat pneumonia caused by aspergillus fumigatus. during a phase of artificial respiration, transfused white cells in peripheral blood and bro ...	1991	1954982
anti-candida resistance in the mouse brain and effect of intracerebral administration of interleukin 1.	the effects of intracerebral and intravenous candida albicans infection on experimental meningo-encephalitis in mice were compared. naive mice inoculated with two c. albicans strains of different pathogenicity (highly virulent ca-6 and poorly virulent pca-2) were more resistant to infection when the yeasts were inoculated by the intracerebral rather than the intravenous route. in immunized mice, in which systemic immunity had been induced by long-term colonization with low-virulence pca-2 cells, ...	1991	1955866
dna fingerprinting of aspergillus fumigatus isolates from patients with aspergilloma.	fourteen sputum isolates of aspergillus fumigatus from glasgow, scotland, including 12 isolates from patients with aspergilloma were dna typed and compared to 31 previously typed isolates. five isolates obtained over a period of 12 months from one aspergilloma patient were of four dna types. two isolates from another patient with aspergilloma were identical. one isolate each from five other aspergilloma patients showed five dna types. two dna types, each shared by two aspergilloma isolates, were ...	1991	1955955
circulating antibodies against thermophilic actinomycetes in farmers and mushroom workers.	sera from individuals exposed to moldy hay, mushroom compost and unexposed individuals, were studied for antibodies against thermophilic actinomycetes and aspergillus fumigatus. antigens extracted from thermoactinomyces vulgaris strains isolated from china were used in the study along with antigens from standard strains to screen the sera of individuals exposed to farms and unexposed controls from both china and the united states. it was found that very little antibody response to a. fumigatus a ...	1991	1960394
potent anti-angiogenic action of agm-1470: comparison to the fumagillin parent.	the anti-angiogenic activity of agm-1470, a new synthetic analog of fumagillin isolated from aspergillus fumigatus, was extensively examined both in vitro and in vivo using four different types of assay and compared to that of the fumagillin parent. locally administered agm-1470 inhibited the angiogenesis in the chick embryo chorioallantoic membrane assay and the rat corneal assay. in the rat sponge implantation assay, systemically administered agm-1470 inhibited angiogenesis induced by basic fi ...	1991	1705118
ultrastructural demonstration of specific igg and ige antibodies binding to aspergillus fumigatus from patients with aspergillosis.	aspergillus-induced diseases usually demonstrate elevated circulating antibodies belonging to different isotypes. the antigens currently used to detect antibodies are crude culture filtrate and mycelial extracts of a. fumigatus (af). most af-associated diseases result from the inhalation of the spores of the organisms present in the environment. however, it is not known whether specific circulating antibodies directed only against spore or mycelia of af exist in the sera of patients with af-indu ...	1991	1706370
crystallization and preliminary characterization of mitogillin, a ribosomal ribonuclease from aspergillus restrictus.	mitogillin is a ribonuclease secreted by the fungus aspergillus restrictus. the substrate for mitogillin is a short, universally conserved, sequence in ribosomal rna. cleavage of this sequence inactivates protein synthesis. mitogillin was crystallized by a two-chamber vapor/liquid diffusion method using ethanol as the precipitant. this method has wider potential in the use of volatile organic solvents as precipitants. crystals of mitogillin diffract x-rays to lattice d-spacings of at least 1.6 a ...	1991	1707977
langerhans cell depletion in gliotoxin-treated murine epidermis.	langerhans cells (lc) are dendritic antigen presenting cells of bone marrow origin which reside in the suprabasal layer of the epidermis. they express high concentrations of class ii mhc glycoproteins on their plasma membrane and transport cutaneous antigen to local lymph nodes for presentation to helper t cells. they are thus essential for the induction of cutaneous immunity. gliotoxin is a member of the epipolythiodioxopiperazine (etp) group of fungal metabolites, derived from the human pathog ...	1991	1712093
protective effect of human granulocyte colony-stimulating factor (hg-csf) on cryptococcus and aspergillus infections in normal and immunosuppressed mice.	prophylactic treatment with human granulocyte colony-stimulating factor (hg-csf) affords significant protection against systemic aspergillosis or pulmonary aspergillosis in neutropenic (cyclophosphamide-treated) mice but not in cortisone-treated animals. cryptococcosis does not respond to hg-csf therapy. our data show that granulocytes play an important role in the immune defense against aspergillosis, but not against cryptococcosis. combined treatment using hg-csf and conventional antimycotics ...	1991	1724550
clinical isolates of yeast produce a gliotoxin-like substance.	candida infections are major causes of morbidity in compromised human hosts, but our understanding of the virulence of candida remains incomplete. the possibility that toxic fungal metabolites belonging to the chemical class epipolythiodioxopiperzine (etp), which are reported to possess immunomodulating and antiphagocytic properties may be produced by candida species was investigated. reversed phase hplc analysis of flash evaporated chloroform extracts of 7 day cultures of clinical candida isola ...	1991	1724551
necrotizing bronchial aspergillosis in a patient receiving neoadjuvant chemotherapy for non-small cell lung carcinoma.	we describe a case of necrotizing bronchial aspergillosis which developed after lobectomy following neoadjuvant chemotherapy in a 73-year-old woman with non-small cell lung cancer. the lesion was visualized and biopsied through fbs, which played a useful role for early diagnosis of this disease. itraconazole therapy was effective and safe.	1991	1647938
[in vitro antifungal activity of itraconazole, a new triazole antifungal agent, against clinical isolates from patients with systemic mycoses].	the in vitro antifungal activity of itraconazole (itz), a new oral triazole antifungal agent, against clinical isolates from patients with systemic mycoses were compared with those of existing systemic antifungals, viz. ketoconazole (kcz), miconazole or amphotericin b. the studies were performed with 65 isolates of pathogenic yeasts and 13 isolates of aspergillus spp. using the agar dilution method on casitone agar. itz showed the most potent antifungal activities against isolates of pathogenic ...	1991	1652654
[the therapeutic effects of itraconazole, a new triazole antifungal agent, for experimental fungal infections].	the therapeutic efficacy of itraconazole (itz), and oral triazole antifungal agent, was studied using several experimental fungal infections in animals. the following results were obtained: 1. ed50 values of itz and ketoconazole (kcz) in a murine model of systemic candidiasis produced by intravenous challenge of candida albicans alls were 32.9 mg/kg and 224 mg/kg, respectively. itz suppressed the proliferation of candida experimentally colonized in the gi-tract of mice and/or a secondary dissemi ...	1991	1652657
treatment of postoperative aspergillus fumigatus spondylodiscitis with itraconazole.	a 46-year-old man presented with aspergillus fumigatus spondylodiscitis after lumbar surgery. he was successfully treated with the new antifungal drug itraconazole in combination with surgical débridement of the disc space. the patient has remained on itraconazole for more than a year and tolerated the drug well.	1991	1652789
disseminated aspergillosis after fungemia in a patient with extragonadal germ cell tumor undergoing autologous bone marrow transplantation.	in a 40-year-old man with extragonadal germ cell tumor, aspergillus fumigatus disseminated hematogenously from a skin infection at a hickman catheter exit site after autologous bone marrow transplantation. a notable aspect of this case was the absence of neutropenia.	1991	1655226
safety and efficacy of itraconazole in prevention of fungal infections in neutropenic patients.	in a non-randomized study the efficacy of itraconazole in preventing fungal infections in neutropenic patients was investigated. forty-seven patients with acute leukemia or advanced lymphoblastic lymphoma were enrolled. ninety-two episodes of severe neutropenia after chemotherapy were observed. mean duration of neutropenia was 24 days. norfloxacin was administered as prophylaxis against gram-negative infections and itraconazole 200 mg b.i.d. as antifungal prophylaxis. surveillance cultures of th ...	1991	1660108
[clinical and immunological features of allergic bronchopulmonary aspergillosis].	28 cases of allergic bronchopulmonary mycosis (abpm) were diagnosed by criteria formulated in 1977 by rosenberg and by referring to the results of serum ige-af and igg-af detection. the common clinical features were wheezing, fever, bloody sputum, sputum plugs, chest pain and loss of energy. on chest x-ray, infiltrates were found in 28 cases, fibrosis in 12, atelectasis in 4, lobar shrinkage in 1, and aspergilloma in 1, chest films in 14 cases showed shadows suggesting in the presence of cb. tom ...	1991	1660774
treatment of pulmonary aspergilloma with itraconazole.	in a 12 month open study of itraconazole in pulmonary aspergilloma nine patients received oral itraconazole 200 mg daily for six months followed by further itraconazole or observation for a further six months. there was no change in the serum igg specific for aspergillus fumigatus (mean (se) change -4% (10%)) or symptoms of chronic cough and haemoptysis. in two of the three patients who continued treatment beyond six months symptoms and radiographic appearances improved and a temporary reduction ...	1991	1663275
pulmonary aspergillosis complicated by osteomyelitis.	we report on a patient with aplastic anaemia and an invasive aspergillosis of the lung with subsequent osteomyelitis of the ribs. diagnosis was made by puncture of the soft tissues and isolation of aspergillus fumigatus. treatment with amphotericin b induced renal function disturbances. it was successfully replaced by ambisome. healing occurred with recovery of the immunity of the patient. the literature on aspergillus osteomyelitis is reviewed.	1991	1665943
an outbreak of aspergillosis in a general itu.	over a 5-month period, six patients in a general intensive therapy unit became colonized by aspergillus species including aspergillus fumigatus, and invasive infection occurred in at least two of them. broncho-alveolar lavage was unhelpful in discriminating between infection and colonization. the source of infection was presumed to be disturbance of an accumulation of spores in fibrous insulation material above the perforated metal ceiling. patients in such units without clearly identifiable def ...	1991	1680899
aspergillus antigen latex test for diagnosis of invasive aspergillosis.		1991	1681333
rapidly progressive respiratory failure due to aspergillus pneumonia: a complication of short-term corticosteroid therapy.		1992	1729033
analysis of aspergillus fumigatus catalases possessing antigenic activity.	analysis of aspergillus fumigatus water soluble fractions by electrophoresis on non-denaturing polyacrylamide gels (page) showed the presence of at least three catalase bands. they were designated f, s1 and s2 in order of descending electrophoretic mobility with respect to the anode. the multiple enzyme forms appear to be distinct in their physicochemical properties. enzyme bands s1 and s2 were simple catalases; the f band had an additional peroxidase function. all of the components were antigen ...	1992	1731061
aspergillus fumigatus sternal wound infections in patients undergoing open heart surgery.	during a 21-month period (july 1986-april 1988), six patients who underwent open heart surgery at holston valley hospital and medical center in kingsport, tennessee, developed sternal would infections caused by aspergillus fumigatus. all patients required sternectomy, reconstructive surgery, and long term amphotericin b therapy; no patient died. by univariate analysis, the following were significantly associated with a. fumigatus sternal would infection: chronic lung disease, a recent history of ...	1992	1736660
granulocyte-colony stimulating factor facilitates the restoration of resistance to opportunistic fungi in leukopenic mice.	administration of recombinant human granulocyte-colony stimulating factor (g-csf), which induces hemopoietic precursor cells to proliferate and differentiate into neutrophils, protected leukopenic mice infected with opportunistic fungi. mice were injected with cyclophosphamide and were then subcutaneously (s.c.) or intravenously (i.v.) administered 15-120 micrograms g-csf kg-1 day-1 for 3 days. the number of peripheral blood neutrophils in the g-csf treated mice was significantly increased 48 h ...	1992	1279154
quantitative preservation of viability of aspergillus fumigatus.	quantitative preservation of the viability of two isolates of aspergillus fumigatus was studied over a 15-month period of storage. significant loss of viability occurred in isolates preserved at -70 degrees c in both phosphate-buffered saline with tween (pbst) and 0.1% gelatin, immediately, and between 6 and 15 months. storage in 10% and 25% dimethyl sulphoxide at -70 degrees c was successful up to 8 weeks with gradual loss of viability later. pbst at 4 degrees c or room temperature, or 10% glyc ...	1992	1287169
[a case of allergic bronchopulmonary aspergillosis with no history of bronchial asthma].	a 53-year-old male was admitted to our hospital because of an abnormal shadow in the left upper lung. bronchofiberscopy revealed edematous mucosa and pus at the orifice of the left upper lobe bronchus. pathological examination revealed bronchial inflammatory change with infiltration of eosinophils and also the existence of aspergillus in the pus. mild eosinophilia and elevation of serum ige level were observed in the peripheral blood, and serum precipitin against aspergillus fumigatus was positi ...	1992	1289634
[filamentous micromycetes in otitis].	in 1984-1991 in the microbiological laboratory in komárno (slovak republic) 4224 (100%) smears with the diagnosis of otitis were examined. from 138 (3.26%) of smears microscopic filamentous fungi were isolated, most frequently aspergillae from the group aspergillus niger (34.1%), aspergillus flavus (29.8%) and aspergillus fumigatus (21.8% of 138 isolates). the strains of other species of filamentous micromycetes were found only rarely. a review on the incidence of filamentous micromycetes in oti ...	1992	1291112
a retrospective clinical comparison between antifungal treatment with liposomal amphotericin b (ambisome) and conventional amphotericin b in transplant recipients.	invasive fungal infections are an important cause of morbidity and mortality in immunosuppressed bone marrow and solid organ transplant recipients. treatment with amphotericin b, the drug of choice for these infections, is however often limited by toxicity. ten transplant patients receiving a liposomal amphotericin b formulation (ambisome) were compared to ten retrospective control patients given conventional amphotericin b. each group included bone marrow (8), kidney (1), and liver transplant ( ...	1992	1291871
[aspergillosis involving the thoracic spinal cord--an autopsy case].	a 63 year old male complained of persistent backache and productive cough. the chest x-ray revealed the fungus ball at the left apical-posterior segment and aspergillus fumigatus was cultured from the sputum. he was treated on fulconazole and miconazole. six months later, motor and sensory paralysis below the mamillary level and urinary and stool incontinence developed. a magnetic resonance image disclosed the destruction of the second thoracic spinal vertebra involved by the cavitated fungus ba ...	1992	1296715
[inhibitory effects of organic acids and salts on selected micromycetes].	inhibitory effects of two fungistatic preparations (a and b) and of propionic acid were tested in a complete feed mixture for broiler chickens (starter mixture). the water content of this mixture was 25.4%, environmental temperature was 24 degrees c and relative air humidity 90%. propionic acid, which has a high fungistatic effect, served as a standard. the composition of the preparation a was as follows: propionic acid, acetic acid, sorbic acid, citric acid and calcium propionate. the preparati ...	1992	1297244
tissue distribution and antifungal effect of liposomal itraconazole in experimental cryptococcosis and pulmonary aspergillosis.	we studied the tissue distribution and in vivo antifungal effect of itraconazole, incorporated into pure dipalmitoylphosphatidylcholine (dppc) multilamellar liposomes and administered intravenously. eighty percent of the itraconazole was associated with dppc. drug levels in lung, brain, and liver, obtained after intravenous administration of tritiated itraconazole, were higher when the drug was administered intravenously as liposomal than when it was dissolved in cyclodextrin. administration of ...	1992	1310577
the evaluation of therapeutic responses in experimental keratomycosis.	two different measures of response to therapy were evaluated in a model of keratitis caused by aspergillus fumigatus in dutch-belted rabbits. combined pre and post-inoculation treatment with oral fluconazole 37.5 mg/kg bid or itraconazole 40 mg/kg bid was compared to post-inoculation treatment only and untreated controls using a standardized clinical disease severity score and quantitative isolate recovery techniques. for both drugs, there was no difference in isolate recovery rates among all th ...	1992	1313750
aspergillus osteomyelitis of the rib: sonographic diagnosis.		1992	1313838
comparison of azoles against aspergilli in vitro and in an experimental model of pulmonary aspergillosis.	current treatment modalities for bronchopulmonary aspergillosis are not very satisfying. we determined the in vitro activity of recently available azoles against aspergillus fumigatus, aspergillus flavus and aspergillus niger. subsequently, these agents were evaluated in an animal model of bronchopulmonary aspergillosis using a. fumigatus as test organism. in vitro, detectable activity was only found for itraconazole (all minimal inhibitory concentrations, mics, less than or equal to 3.2 microgr ...	1992	1317280
[primary aspergillus endocarditis. apropos of a case and review of the international literature].	the authors report a case of primary aspergillus endocarditis with endophthalmitis and vertebral osteomyelitis. no underlying disease and no predisposing factors were found. valve replacement plus combined antifungal chemotherapy proved to be effective as the patient is asymptomatic 18 months after the first symptoms. 48 cases of aspergillus endocarditis, without prior cardiac surgery have been reported in the literature. aspergillus endocarditis was valvular or mural. extracardiac dissemination ...	1992	1318668
[sphenoid sinus aspergillosis presenting abducens nerve palsy and visual field impairment; a case report].	a case of sphenoid sinus aspergillosis presenting abducens nerve palsy and visual field impairment is reported. a 73-year-old woman visited our hospital with the complaint of head heaviness on the 27th of march, 1989. although results of neurological examinations were normal, craniogram revealed the destruction of the clivus, and ct scan and mri showed a mass lesion, which was thought to be a mucocele in the sphenoid sinus. on the 1st of september, she developed right abducens nerve palsy and vi ...	1992	1321350
diagnosis of avian aspergillosis and treatment with itraconazole.		1992	1322581
effects of culture media on the in vitro susceptibility of selected opportunistic fungi to fluconazole and itraconazole.	the sensitivity of 23 isolates of opportunistic fungi, aspergillus fumigatus (5), a. flavus (5), a. niger (5), pseudallescheria boydii (5), alternaria alternata (2) and xylohypha bantiana (1), was investigated against fluconazole and itraconazole, using sabouraud's dextrose broth (sd) and a high-resolution (hr) medium (pfizer, inc.). the procedure followed was a standard tube dilution (1 ml/tube) method. candida albicans y01 09 was included as reference strain to monitor quality and reproducibil ...	1992	1324830
possible failure of itraconazole in preventing fungal infection in a neutropenic leukaemia patient.		1992	1327788
the occurrence of fungi in bovine tissues in relation to portals of entry and environmental factors.	the occurrence of fungi in tissue specimens from 72 cattle was examined by culture, histopathology and indirect immunofluorescence staining (iif). groups of 12 animals each had been fed either concentrate or roughage and had been housed either in tie stalls, on slatted floors or on deep bedding. specimens were obtained from the lung, omasum and peyer's patches of the ileum and corresponding lymph nodes. both hyphae and spores were made visible by iif and by combination of iif, morphology and con ...	1992	1333494
aspergillus-induced discitis. a role for itraconazole in therapy?		1992	1335170
aspergillus hepatosplenic abscesses and chylous ascites: successful treatment with fluconazole.		1992	1341456
[the isolation and evaluation of aspergillus fumigatus antigens].	antigens from three strains of aspergillus fumigatus (354, 356, and jig) and an antiserum against the mixing of these antigens have been produced, and evaluated immunochemically. the antigens were obtained through a modified coleman & kaufman technique (culture filtrate concentrated by acetone). analysis by the immunodiffusion test (id) against homologous serum has yielded 100% sensitivity (with the studied sera). concerning heterologous sera we found reactivity with a serum of a patient of cand ...	1992	1342095
restriction endonuclease analysis of aspergillus fumigatus dna.	to develop a genome based dna fingerprinting system for aspergillus fumigatus mould.	1992	1349614
sudden infant death syndrome (sids) and the toxic gas hypothesis: microbiological studies of cot mattresses.	1. fifty infants' mattresses were studied to investigate the occurrence of viable fungal and bacterial propagules, with particular reference to scopulariopsis brevicaulis which had been suggested to be implicated in sids cases. a total of 19 sids cases mattresses, 1 non-sids death, 20 used controls, and 10 new unused controls were examined. 2. differences were found between sids and used controls in the variety of fungal species isolated and the numbers isolated from fillings; bacterial numbers ...	1992	1358149
use of randomly amplified polymorphic dna markers to distinguish isolates of aspergillus fumigatus.	forty-four oligonucleotide decamers were tested for their abilities to generate randomly amplified polymorphic dna (rapd) markers from genomic dnas of three different isolates of aspergillus fumigatus. seven primers generated rapds that allowed the three isolates to be differentiated; one of the primers also yielded a unique rapd pattern in each of an additional six fungal isolates, demonstrating the utility of this technique for distinguishing between a. fumigatus isolates.	1992	1360475
[exogenous allergic alveolitis: assessment of antigen-specific igg antibodies using a new chemiluminescence method].	the demonstration of elevated levels of antigen-specific igg antibodies is of importance for the diagnostic workup of patients with a suspected hypersensitivity pneumonitis. we established a solid phase immunoassay for the determination of these antibodies based on chemiluminescence (clia). the clia demonstrated a low level of unspecific binding. the reproducibility of the clia was evaluated by calculating the intra- and inter-variation; the variation coefficient was 4% (intra-variation) resp. 1 ...	1992	1372432
abnormal humoral immune response to mucosal antigenic stimulation in patients with lung cancer.	previous studies have suggested an inverse relationship between atopy and cancer of mucosal surfaces. atopy is classically assessed by detecting specific immunoglobulin e (ige) antibodies against inhalant allergens. however, platts-mills recently proposed that atopy is the ability of an organism to recognize and to respond to limited doses of allergens presented at the mucosal level by producing not only ige, but also immunoglobulin a and immunoglobulin g (igg) antibodies. the authors compared t ...	1992	1373339
rat monoclonal antibodies against aspergillus galactomannan.	monoclonal antibodies (mabs) against aspergillus fumigatus galactomannan were produced in rats. seven of them, eb-a1 through eb-a7, were characterized in more detail. they were all immunoglobulin m antibodies, reacting in an indirect enzyme-linked immunosorbent assay with purified a. fumigatus galactomannan, with avidity constants of between 2 x 10(9) and 5 x 10(9)/m. enzyme-linked immunosorbent assay inhibition experiments with modified galactomannan and synthetic oligomers of beta (1----5)gala ...	1992	1375195
characterization of a monoclonal antibody (p40) against the 68 kd major allergen of penicillium notatum.	a monoclonal antibody (moab p40) against the 68 kd major allergen of penicillium notatum (p. notatum) was obtained by immunizing the mouse with a crude extract of p. notatum. analysed by two-dimensional gel electrophoresis and immunoblotting, p40 reacted with two different isoforms of the 68 kd component of p. notatum with pis of 5.4 and 5.5. in addition to p. notatum, p40 showed positive elisa activity to aspergillus fumigatus (a. fumigatus) but not to components of six other fungi including al ...	1992	1377093
copper organo-chelators in aspergillus fumigatus and penicillium chrysogenum.	the presence of copper in the growth medium induces the biosynthesis of several low-mol-wt copper ogano-chelators in aspergillus fumigatus and penicillium chrysogenum. by use of p. chrysogenum, we were able to prepare several low-mol-wt metallothionein chelators, as well as several short-chain copper chelatins. the amino acid composition of a chelatin of mol wt approx 799 da revealed the presence of cysteine, glutamic acid, glycine, and leucine. use of a. fumigatus led to fewer copper chelators ...	1992	1381937
[invasive aspergillosis in acquired immunodeficiency syndrome].		1992	1391009
[the radiological diagnosis of maxillary sinus aspergillosis].		1992	1396144
exophiala dermatitidis pneumonia in cystic fibrosis.	the chest x-ray film of a girl with cystic fibrosis (cf) showed slowly increasing mottled densities during the 6th and 7th year of her life. pulmonary symptoms and distress proceeded fast in spite of intensive treatment with antibiotics, corticosteroids, and physiotherapy. three different fungal organisms were repeatedly cultured from the sputum: candida albicans, aspergillus fumigatus, and exophiala dermatitidis. antibodies against c. albicans were in the normal range. candida antigen in blood ...	1992	1396889
specific serum immunopatterns in clinical phases of allergic bronchopulmonary aspergillosis.	immunoblotting, radioallergosorbent test (rast), and enzyme-linked immunosorbent assay (elisa) were performed to determine specific ige and igg responses to aspergillus fumigatus (af) allergens (ige-af; igg-af). serology results were compared in patients with allergic bronchopulmonary aspergillosis (abpa) (n = 43), patients with aspergillus fumigatus-associated asthma (af-asthma) (n = 26), and healthy individuals (n = 3). in patients with different clinical phases of abpa, three specific immunop ...	1992	1401642
[limulus test (factor g) and polysaccharides from fungus].	picogram quantifies per ml of endotoxin showed a positive limulus test. the concentration more than ten pg/ml of curdlan ((1-3)-beta-d-glucan) was also positive to a conventional limulus test, which contains a factor g. one nanogram per ml of cm-curdlan was positive, but higher concentration of 10 pg/ml of cm-curdlan decreased its optical density of the conventional limulus test. more than one hundred ng/ml of mannan from saccharomyces cerevisiae did not react with factor g. however, a culture s ...	1992	1402105
[physiopathology and diagnosis of allergic bronchopulmonary aspergillosis].		1992	1402236
aspergillus infection of total knee arthroplasty presenting as a popliteal cyst. case report and review of the literature.	fungal infections have only rarely been reported to occur in patients having undergone total knee arthroplasty. this case report documents the first known case of aspergillus fumigatus as the offending organism. its initial presentation as a popliteal cyst further reinforces the known association of popliteal cysts and intra-articular knee pathology.	1992	1402949
[a case of chronic necrotizing pulmonary aspergillosis].	a 70-year-old woman with diabetes mellitus who was following a therapeutic diet showed an infiltrative shadow in the right upper lung field on chest roentgenogram in april, 1986. she was diagnosed as having pneumonia and was treated for five months with several antibiotics, but the abnormal shadow on chest roentgenograms increased in size. therefore, she was admitted to our hospital in october 1986. although tubercle bacilli were not isolated from her sputum or from materials obtained by broncho ...	1992	1405108
therapeutic and prophylactic effects of romurtide against experimental animal infections.		1992	1414593
pathogenicity differences of multiple isolates of aspergillus fumigatus in turkeys.	sixteen aspergillus fumigatus isolates of environmental, mammalian, and avian origin were used to assess: 1) intra-air-sac inoculation as a viable challenge alternative to aerosol exposure, and 2) isolate variability in pathogenicity. development of lesions, antibody response in survivors, mortality, and weight gains were assessed. turkey poults were challenged with equal numbers of viable conidia. total number of conidia given per experimental group varied markedly and did not influence mortali ...	1992	1417585
isolated pulmonary aspergillar infection in cardiac transplant recipients: case report and review.	the optimal type and duration of antifungal therapy for localized aspergillosis in cardiac transplant recipients is unclear. we report the case of a patient who had persistent invasive pulmonary aspergillosis despite prolonged treatment with amphotericin b. a combined approach using antifungal therapy and surgery appears effective for localized pulmonary disease. aspergillar infection in solid organ transplant recipients is reviewed.	1992	1420684
aspergillosis of the cns presenting as aseptic meningitis.		1992	1420694