Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| a diagnostic protocol to identify water buffaloes (bubalus bubalis) vaccinated with brucella abortus strain rb51 vaccine. | the use of live vaccine strain rb51 for vaccination of domestic water buffaloes (bubalus bubalis) at risk of infection with brucella abortus is permitted notwithstanding the plans for the eradication and only under strict veterinary control. the antibodies induced by rb51 vaccination are not detectable using conventional diagnostic techniques; therefore, it is necessary to have a specific diagnostic tool able to discriminate vaccinated from unvaccinated animals. the combination of a complement f ... | 2015 | 26129660 |
| novel sequence types of chlamydia pecorum infect free-ranging alpine ibex (capra ibex) and red deer (cervus elaphus) in switzerland. | chlamydia pecorum, a recognized pathogen of domesticated ruminants and koalas (phascolarctos cinereus), has been recently reported in a broad range of other wildlife species including water buffalo (bubalus bubalis), ibex (capra ibex), chamois (rupicapra rupicapra), red deer (cervus elaphus), and birds. this identification raises questions as to whether cross-host transmission may be a factor in the epidemiology of infections in these species. to begin to address this question, we employed a c. ... | 2015 | 25647593 |
| molecular detection of coxiella burnetii using an alternative loop-mediated isothermal amplification assay (lamp). | q fever, caused by coxiella burnetii, is a worldwide zoonosis with important consequences for human and animal health. in livestock, the diagnosis, using direct and indirect techniques, is challenging even if to tackle coxiellosis in domesticated animals a rapid diagnosis is crucial. in the recent years, new molecular methods have been developed to overcome these issues. several polymerase chain reaction (pcr) assays have been studied, but loop mediated isothermal amplification (lamp) has not be ... | 2015 | 25842216 |
| molecular characterization of a new microvariant of the g3 genotype for echinococcus granulosus in water buffalo in iran. | in this study, molecular characterization of echinococcus granulosus sample obtained from water buffalo originating from southwest of iran was performed using comparative sequence analysis of cox1 mitochondrial gene. dna was extracted from protoscoleces removed from hydatid cyst from the liver of a 2-year-old male buffalo slaughtered in khuzestan province. molecular and phylogenetic analyses were conducted based on cox1 mitochondrial gene. we found the presence of a new microvariant of g3 genoty ... | 2015 | 25992257 |
| diversity of lactic acid bacteria isolated from brazilian water buffalo mozzarella cheese. | the water buffalo mozzarella cheese is a typical italian cheese which has been introduced in the thriving brazilian market in the last 10 y, with good acceptance by its consumers. lactic acid bacteria (lab) play an important role in the technological and sensory quality of mozzarella cheese. in this study, the aim was to evaluate the diversity of the autochthones viable lab isolated from water buffalo mozzarella cheese under storage. samples were collected in 3 independent trials in a dairy indu ... | 2015 | 25597646 |
| phylogenetic group determination of escherichia coli isolated from animals samples. | this study analyzes the occurrence and distribution of phylogenetic groups of 391 strains of escherichia coli isolated from poultry, cattle, and water buffalo. the frequency of the phylogroups was a = 19%, b1 = 57%, b2 = 2.3%, c = 4.6%, d = 2.8%, e = 11%, and f = 3.3%. phylogroups a (p < 0.001) and f (p = 0.018) were associated with e. coli strains isolated from poultry, phylogroups b1 (p < 0.001) and e (p = 0.002) were associated with e. coli isolated from cattle, and phylogroups b2 (p = 0.003) ... | 2015 | 26421310 |
| molecular phylogenetic analysis of fasciola flukes from eastern india. | fasciola flukes from eastern india were characterized on the basis of spermatogenesis status and nuclear its1. both fasciola gigantica and aspermic fasciola flukes were detected in imphal, kohima, and gantoku districts. the sequences of mitochondrial nad1 were analyzed to infer their phylogenetical relationship with neighboring countries. the haplotypes of aspermic fasciola flukes were identical or showed a single nucleotide substitution compared to those from populations in the neighboring coun ... | 2015 | 25936970 |
| high prevalence of schistosoma japonicum and fasciola gigantica in bovines from northern samar, the philippines. | the cause of zoonotic schistosomiasis in the philippines is schistosoma japonicum, which infects up to 46 mammalian hosts, including humans and bovines. in china, water buffaloes have been identified as major reservoir hosts for schistosomiasis japonica, contributing up to 75% of human transmission. in the philippines, water buffaloes (carabao; bubalus bubalis carabanesis) have, historically, been considered unimportant reservoirs. we therefore revisited the possible role of bovines in schistoso ... | 2015 | 25643317 |
| anthelmintic effect of biocompatible zinc oxide nanoparticles (zno nps) on gigantocotyle explanatum, a neglected parasite of indian water buffalo. | helminth parasites of veterinary importance cause huge revenue losses to agrarian economy worldwide. with the emergence of drug resistance against the current formulations, there is a need to focus on the alternative approaches in order to control this menace. in the present study, biocompatible zinc oxide nanoparticles (zno nps) were used to see their in vitro effect on the biliary amphistomes, gigantocotyle explanatum, infecting bubalus bubalis because these nanoparticles are involved in gener ... | 2015 | 26177503 |
| somatic antigens of tropical liver flukes ameliorate collagen-induced arthritis in wistar rats. | parasitic helminths polarize immune response of their vertebrate hosts towards anti-inflammatory th2 type and therefore it is hypothesized that they may suppress the inflammatory conditions in autoimmune disorders. the present study was undertaken to investigate in vivo immunomodulatory and therapeutic potential of somatic antigens (ag) of liver infecting digenetic trematodes [fasciola gigantica (fg) and gigantocotyle explanatum (ge)] in collagen-induced arthritic (cia) wistar rats. the cia rats ... | 2015 | 25992888 |
| large-scale serological survey of bovine ephemeral fever in china. | bovine ephemeral fever (bef) is caused by the arthropod-borne bovine ephemeral fever virus (befv), which is classified in the family rhabdoviridae and the genus ephemerovirus. a debilitating and sometimes fatal viral disease, bef affects cattle and water buffalo. the epizootiology of bef among cattle in china has not been fully determined. we examined the seroprevalence of the befv among cattle in china between january 2012 and june 2014. among the 2822 serum samples collected from various cattl ... | 2015 | 25595266 |
| seroprevalence of bovine ephemeral fever virus in domesticated and wildlife species during epidemic and inter-epidemic periods (2000-2009) in israel. | bovine ephemeral fever (bef) is an economically important vector-borne viral disease of cattle and buffalo. it has been reported from most of the world's tropical and subtropical regions. in the last few decades, outbreaks of bef have occurred in israel almost every other year. several serological studies have demonstrated a wide range of wild animal species that are positive for bef virus (befv) antibodies. however, the question of whether wild animals and domesticated species other than cattle ... | 2015 | 23675891 |
| molecular detection of bovine immunodeficiency virus in water buffaloes (bubalus bubalis) from the amazon region, brazil. | bovine immunodeficiency is a chronic progressive disease caused by a lentivirus that affects cattle and buffaloes. although the infection has been described in cattle in some countries, including in brazil, there are only two reports of infection in buffaloes: one in pakistan and one in cambodia. the aim of the present study was to survey the occurrence of bovine immunodeficiency virus (biv) in water buffaloes from the amazon region, pará state, brazil. biv proviral dna was surveyed in 607 whole ... | 2015 | 26174574 |
| molecular analyses detect natural coinfection of water buffaloes (bubalus bubalis) with bovine viral diarrhea viruses (bvdv) in serologically negative animals. | infection of water buffaloes (bubalus bubalis) with bovine viral diarrhea viruses (bvdv) has been confirmed in several studies by serological and molecular techniques. in order to determine the presence of persistently infected animals and circulating species and subtypes of bvdv we conducted this study on a buffalo herd, whose habitat was shared with bovine cattle (bossp.). our serological results showed a high level of positivity for bvdv-1 and bvdv-2 within the buffalo herd. the molecular ana ... | 2015 | 25962538 |
| prevalence study and genetic typing of bovine viral diarrhea virus (bvdv) in four bovine species in china. | to determine the nationwide status of persistent bvdv infection in different bovine species in china and compare different test methods, a total of 1379 serum samples from clinical healthy dairy cattle, beef cattle, yaks (bos grunniens), and water buffalo (bubalus bubalis) were collected in eight provinces of china from 2010 to 2013. the samples were analyzed using commercial antibody (ab) and antigen (ag) detection kits, and rt-pcr based on the 5'-utr and npro gene sequencing. results showed th ... | 2015 | 25849315 |
| characterization and annotation of babesia orientalis apicoplast genome. | babesia orientalis is an obligate intraerythrocytic protozoan parasite of the buffalo (bubalus bubalis, linnaeus, 1758) transmitted by the tick rhipicephalus heamaphysaloides. it is the causative agent of water buffalo babesiosis, one of the most important pathogens of water buffalo in central and southern china. as a member of the phylum apicomplexa, b. orientalis possesses a relatively independent and alga originated organelle the apicoplast. apicoplasts in other apicomplexa parasites are invo ... | 2015 | 26474853 |
| identification and characterization of a novel 34 kda merozoite protein in babesia orientalis. | a novel babesia orientalis 34 kda protein (designated bop34) was obtained by immunoscreening of a cdna expression library using b. orientalis infected water buffalo serum. the complete nucleotide sequence of the bop34 was 1088 bp, which contained one open reading frame (orf), two untranslated regions (utrs) and a poly (a) tail. the length of orf was 933 bp, encoding a polypeptide of 310 aa with a predicted size of 34 kda. blast analysis showed that the nucleotide sequence of bop34 had 71% simila ... | 2015 | 26169218 |
| genetic variations in merozoite surface antigen genes of babesia bovis detected in vietnamese cattle and water buffaloes. | the genes that encode merozoite surface antigens (msas) in babesia bovis are genetically diverse. in this study, we analyzed the genetic diversity of b. bovis msa-1, msa-2b, and msa-2c genes in vietnamese cattle and water buffaloes. blood dna samples from 258 cattle and 49 water buffaloes reared in the thua thien hue province of vietnam were screened with a b. bovis-specific diagnostic pcr assay. the b. bovis-positive dna samples (23 cattle and 16 water buffaloes) were then subjected to pcr assa ... | 2015 | 25575442 |
| mitochondrial dna variability of domestic river buffalo (bubalus bubalis) populations: genetic evidence for domestication of river buffalo in indian subcontinent. | river buffalo, bubalus bubalis is a large bovine species frequently used livestock in southern asia. it is believed that the river buffalo was domesticated from bubalus arnee, the wild buffalo of mainland asia, a few thousand years ago, probably during the period of indus valley civilization. however, the domestication history of the river buffalo has been the subject of debate for many decades mainly due to the lack of clear archeological evidence and the divisive conclusions of the genetic stu ... | 2015 | 25900921 |
| microphthalmia-associated transcription factor mutations are associated with white-spotted coat color in swamp buffalo. | a candidate gene analysis of the microphthalmia-associated transcription factor (mitf) gene was used in an attempt to identify the genetic basis for a white-spotted coat color phenotype in the asian swamp buffalo (bubalus bubalis carabanensis). ninety-three buffaloes-32 solid, 38 spotted and 23 white individuals-were sanger-sequenced for all mitf exons as well as highly conserved intronic and flanking regions. mitf cdna representing skin and iris tissue from six spotted, nine solid and one white ... | 2015 | 26417640 |
| genome-wide survey and analysis of microsatellite sequences in bovid species. | microsatellites or simple sequence repeats (ssrs) have become the most popular source of genetic markers, which are ubiquitously distributed in many eukaryotic and prokaryotic genomes. this is the first study examining and comparing ssrs in completely sequenced genomes of the bovidae. we analyzed and compared the number of ssrs, relative abundance, relative density, guanine-cytosine (gc) content and proportion of ssrs in six taxonomically different bovid species: bos taurus, bubalus bubalis, bos ... | 2015 | 26196922 |
| ocular fluid as a replacement for serum in cell cryopreservation media. | cryostorage is of immense interest in biomedical research, especially for stem cell-based therapies and fertility preservation. several protocols have been developed for efficient cryopreservation of cells and tissues, and a combination of dimethyl sulfoxide (dmso) and fetal bovine serum (fbs) is commonly used. however, there is a need for an alternative to fbs because of ethical reasons, high cost, and risk of contamination with blood-borne diseases. the objective of the present study was to ex ... | 2015 | 26135924 |
| the differential expression of immune genes between water buffalo and yellow cattle determines species-specific susceptibility to schistosoma japonicum infection. | water buffalo are less susceptible to schistosoma japonicum infection than yellow cattle. the factors that affect such differences in susceptibility remain unknown. a bos taurus genome-wide gene chip was used to analyze gene expression profiles in the peripheral blood of water buffalo and yellow cattle pre- and post-infection with s. japonicum. this study showed that most of the identified differentially expressed genes (degs) between water buffalo and yellow cattle pre- and post-infection were ... | 2015 | 26125181 |
| effect of roughage on rumen microbiota composition in the efficient feed converter and sturdy indian jaffrabadi buffalo (bubalus bubalis). | the rumen microbiota functions as an effective system for conversion of dietary feed to microbial proteins and volatile fatty acids. in the present study, metagenomic approach was applied to elucidate the buffalo rumen microbiome of jaffrabadi buffalo adapted to varied dietary treatments with the hypothesis that the microbial diversity and subsequent in the functional capacity will alter with diet change and enhance our knowledge of effect of microbe on host physiology. eight adult animals were ... | 2015 | 26714477 |
| generation of a novel bacteriophage library displaying scfv antibody fragments from the natural buffalo host to identify antigens from adult schistosoma japonicum for diagnostic development. | the development of effective diagnostic tools will be essential in the continuing fight to reduce schistosome infection; however, the diagnostic tests available to date are generally laborious and difficult to implement in current parasite control strategies. we generated a series of single-chain antibody fv domain (scfv) phage display libraries from the portal lymph node of field exposed water buffaloes, bubalus bubalis, 11-12 days post challenge with schistosoma japonicum cercariae. the select ... | 2015 | 26684756 |
| activation and inhibition of the wnt3a signaling pathway in buffalo (bubalus bubalis) embryonic stem cells: effects of wnt3a, bio and dkk1. | this research studies the effects of activation and inhibition of wnt3a signaling pathway in buffalo (bubalus bubalis) embryonic stem (es) cell-like cells. | 2015 | 26644860 |
| short communication: arcobacter butzleri and arcobacter cryaerophilus survival and growth in artisanal and industrial ricotta cheese. | ricotta cheese is a ready-to-eat product with properties (ph >6.0, aw >0.98-0.99) and moisture content (75-80%) that may pose a risk to public health due to postprocess contamination by several bacterial pathogens, including arcobacters. the objective of the study was to evaluate the behavior of arcobacter butzleri and arcobacter cryaerophilus in ricotta cheese during its shelf life assuming postprocessing contamination. two types of ricotta cheese, artisanal water buffalo (wb) and industrial co ... | 2015 | 26233450 |
| proteomic based approach for characterizing 4-hydroxy-2-nonenal induced oxidation of buffalo (bubalus bubalis) and goat (capra hircus) meat myoglobins. | myoglobin (mb) is a sarcoplasmic heme protein primarily responsible for meat color and its chemistry is species specific. 4-hydroxy-2-nonenal (hne) is a cytotoxic lipid derived aldehyde detected in meat and was reported to covalently adduct with nucleophilic histidine residues of mb and predispose it to greater oxidation. however, no literature is available on characterization of lipid oxidation induced oxidation of indian water buffalo (bubalus bubalis) and goat (capra hircus) myoglobins. | 2016 | 27891064 |
| de novo transcriptome assembly of the chinese swamp buffalo by rna sequencing and ssr marker discovery. | the chinese swamp buffalo (bubalis bubalis) is vital to the lives of small farmers and has tremendous economic importance. however, a lack of genomic information has hampered research on augmenting marker assisted breeding programs in this species. thus, a high-throughput transcriptomic sequencing of b. bubalis was conducted to generate transcriptomic sequence dataset for gene discovery and molecular marker development. illumina paired-end sequencing generated a total of 54,109,173 raw reads. af ... | 2016 | 26766209 |
| cattle are a potential reservoir of bubaline herpesvirus 1 (buhv1). | in the present work, controlled experimental infection and transmission studies in domestic cattle (bos taurus) and water buffaloes (bubalus bubalis) were carried out to study the in vivo behaviour of bubaline herpesvirus 1 (buhv1). two bovine and two buffalo calves were infected with buhv1 (20287n isolate) by intranasal aerosolisation. two sentinel cattle did not receive the virus challenge, but were housed with infected buffaloes to evaluate horizontal transmission. all experimentally inoculat ... | 2016 | 28074144 |
| lessons for livestock genomics from genome and transcriptome sequencing in cattle and other mammals. | decreasing sequencing costs and development of new protocols for characterizing global methylation, gene expression patterns and regulatory regions have stimulated the generation of large livestock datasets. here, we discuss experiences in the analysis of whole-genome and transcriptome sequence data. | 2016 | 27534529 |
| comparative analysis of snp candidates in disparate milk yielding river buffaloes using targeted sequencing. | river buffalo (bubalus bubalis) milk plays an important role in economy and nutritious diet in several developing countries. however, reliable milk-yield genomic markers and their functional insights remain unexposed. here, we have used a target capture sequencing approach in three economically important buffalo breeds namely: banni, jafrabadi and mehsani, belonging to either high or low milk-yield group. blood samples were collected from the milk-yield/breed balanced group of 12 buffaloes, and ... | 2016 | 27441113 |
| identification of the microrna repertoire in tlr-ligand challenged bubaline pbmcs as a model of bacterial and viral infection. | in the present study, we used high-throughput sequencing, mirna-seq, to discover and explore the expression profiles of known and novel mirnas in tlr ligand-stimulated vis-à-vis non-stimulated (i.e. control) peripheral blood mononuclear cells (pbmcs) isolated from blood of healthy murrah buffaloes. six small rna (srna) libraries were multiplexed in ion torrent pi chip and sequenced on ion proton system. the reads obtained were aligned to the bos taurus genome (umd3.1 assembly), which is phylogen ... | 2016 | 27257788 |
| ungulate malaria parasites. | haemosporida parasites of even-toed ungulates are diverse and globally distributed, but since their discovery in 1913 their characterization has relied exclusively on microscopy-based descriptions. in order to bring molecular approaches to bear on the identity and evolutionary relationships of ungulate malaria parasites, we conducted plasmodium cytb-specific nested pcr surveys using blood from water buffalo in vietnam and thailand, and goats in zambia. we found that plasmodium is readily detecta ... | 2016 | 26996979 |
| molecular assays reveal the presence of theileria spp. and babesia spp. in asian water buffaloes (bubalus bubalis, linnaeus, 1758) in the amazon region of brazil. | approximately 50% of buffalo herds in brazil are located in pará state in northern brazil. there are several properties where cattle and buffalo live and graze together, and thus, buffalo pathogens may threaten the health of cattle and vice versa. therefore, knowledge of infectious agents of buffalo is essential for maintaining healthy livestock. clinical disease caused by theileria and babesia parasites in the asian water buffalo is not common, although these animals may act as reservoir hosts, ... | 2016 | 27344507 |
| epidemiology of bovine hemoprotozoa parasites in cattle and water buffalo in vietnam. | a pcr-based survey of hemoprotozoa parasites detected babesia bigemina, theileria orientalis and trypanosoma theileri among cattle and water buffalo in vietnam, and a new babesia sp. closely related to babesia ovata was detected in cattle only. in addition, theileria annulata and trypanosoma evansi were not detected in both cattle and water buffalo. phylogenetic analysis detected t. orientalis mpsp genotypes 3, 5, 7 and n3 in cattle and 5, 7, n1 and n2 in water buffalo. additionally, water buffa ... | 2016 | 27149894 |
| first genome sequences of buffalo coronavirus from water buffaloes in bangladesh. | we report the complete genome sequences of a buffalo coronavirus (bufcov hku26) detected from the faecal samples of two domestic water buffaloes (bubalus bubalis) in bangladesh. they possessed 98-99% nucleotide identities to bovine coronavirus (bcov) genomes, supporting bufcov hku26 as a member of betacoronavirus 1. nevertheless, bufcov hku26 possessed distinct accessory proteins between spike and envelope compared to bcov. sugar-binding residues in the n-terminal domain of s protein in bcov are ... | 2016 | 27274850 |
| detection of campylobacter in human and animal field samples in cambodia. | campylobacter are zoonotic bacteria and a leading cause of human gastroenteritis worldwide with campylobacter jejuni and c. coli being the most commonly detected species. the aim of this study was to detect campylobacter in humans and livestock (chickens, ducks, pigs, cattle, water buffalo, quail, pigeons and geese) in rural households by routine culturing and multiplex pcr in faecal samples frozen before analysis. of 681 human samples, 82 (12%) tested positive by pcr (c. jejuni in 66 samples an ... | 2016 | 26991032 |
| risk analysis and seroprevalence of bovine ephemeral fever virus in cattle in the kingdom of saudi arabia. | bovine ephemeral fever virus (befv) is an arthropod-borne rhabdovirus that causes disabling clinical signs and major economic losses in cattle and water buffalo. the disease is well documented in asia, africa, and the middle east; however, the seroprevalence of befv in different regions and bovine breeds in the kingdom of saudi arabia (ksa) is unknown. the aim of this study was to analyze risk factors which affect the prevalence of antibodies against befv in small herds of cattle in four geograp ... | 2016 | 26676243 |
| 2d-page analysis of the soluble proteins of the tropical liver fluke, fasciola gigantica and biliary amphistome, gigantocotyle explanatum, concurrently infecting bubalus bubalis. | the digenetic trematodes, fasciola gigantica and gigantocotyle explanatum, belonging to the family fasciolidae and paramphistomidae respectively, have been often found to concurrently infect the liver of indian water buffalo bubalus bubalis, causing serious pathological damage to the vital organ, incurring huge economic losses. in the present study the soluble gene products of both f. gigantica and g. explanatum were analyzed by 2 dimensional polyacrylamide gel electrophoresis. the soluble prote ... | 2016 | 27605808 |
| molecular characterization and phylogenetic analysis of fasciola gigantica from western java, indonesia. | fasciola gigantica and aspermic (hybrid) fasciola flukes are thought to be distributed in southeast asian countries. the objectives of this study were to investigate the distribution of these flukes from unidentified ruminants in western java, indonesia, and to determine their distribution history into the area. sixty fasciola flukes from western java were identified as f. gigantica based on the nucleotide sequences of the nuclear phosphoenolpyruvate carboxykinase (pepck) and dna polymerase delt ... | 2016 | 27266482 |
| intraspecific and interspecific genetic variation of gongylonema pulchrum and two rodent gongylonema spp. (g. aegypti and g. neoplasticum), with the proposal of g. nepalensis n. sp. for the isolate in water buffaloes from nepal. | the gullet worm (gongylonema pulchrum) has been recorded from a variety of mammals worldwide. in an earlier study, we demonstrated two separate transmission cycles in cattle (bos taurus) and wild mammals in japan based on nucleotide sequences of the ribosomal rna gene (rdna) and cytochrome c oxidase subunit i (cox-1) region of mitochondrial dna of multiple isolates of different origins. our earlier study additionally demonstrated two major cox-1 haplotypes of g. pulchrum prevalent in cattle in j ... | 2016 | 26531300 |
| molecular detection of mycobacterium tuberculosis in cattle and buffaloes: a cause for public health concern. | tuberculosis is a re-emerging disease causing a growing public health burden. the current study was conducted to investigate the occurrence of mycobacterium tuberculosis among cattle and buffaloes with tuberculous lesions. typical tuberculous lesions were collected from 34 cattle and 34 buffaloes (bubalus bubalis) through postmortem examination of slaughtered animals in abattoirs. dnas were extracted from samples, and m. tuberculosis was identified by pcr. positive samples were examined for resi ... | 2016 | 27517579 |
| paratuberculosis in buffaloes in northeast brazil. | several farms in the northeast of brazil were investigated for mycobacterium avium subsp. paratuberculosis infection in order to identify the occurrence of paratuberculosis in buffaloes. samples were obtained from 17 farms, two slaughter houses, and a quarantine area in the northeast. about 15,000 buffaloes of the murrah, mediterranean, and jafarabadi breed as well as their crossbreeds were evaluated for meat, dairy, and mixed farms with semi-intensive or extensive breeding practices. for diagno ... | 2016 | 27334632 |
| inhibitory potential of buffalo (bubalus bubalis) colostrum immunoglobulin g on klebsiella pneumoniae. | the unique components of colostrum like free oligosaccharides and glycoconjugates are known to offer resistance to enzymatic digestion in the gastrointestinal tract and have the ability to inhibit the localized adherence of enteropathogens to the digestive tract of the neonates. in this context, we have evaluated the in vitro effect of buffalo colostrum immunoglobulin g on human pathogen klebsiella pneumoniae, a predominant multidrug resistant pathogen associated with nasocomial infections. the ... | 2016 | 27017977 |
| rumen adaptation of swamp buffaloes (bubalus bubalis) by high level of urea supplementation when fed on rice straw-based diet. | four rumen-fistulated swamp buffaloes (bubalus bubalis) were randomly allocated to investigate rumen adaptation of urea on feed intake, nutrient digestibility, fermentation efficiency, and microbial protein synthesis. buffaloes were fed with rice straw ad libitum for a period of 2 weeks and then were shifted to a step-up diet regimen by supplementation of concentrate containing 20 and 40 g/kg urea at 5 g/kg bw for a period of 2 and 4 weeks, respectively. the results revealed that feed intake and ... | 2016 | 27107749 |
| influence of cutting and deboning operations on the microbiological quality and shelf life of buffalo meat. | considering the specific biochemical composition of buffalo (bubalus bubalis) meat (high iron content, high biological value proteins and essential fatty acids, low amounts of fat and cholesterol), we evaluated the influence of cutting and deboning operations on the microbiological quality and shelf-life of vacuum-packed buffalo meat stored under refrigeration. on the processing day, samples were collected from carcass, deboning room surfaces and meat cuts. samples from meat cuts were evaluated ... | 2016 | 26897086 |
| g-protein-coupled chemokine receptor gene in lumpy skin disease virus isolates from cattle and water buffalo (bubalus bubalis) in egypt. | lumpy skin disease virus (lsdv), sheep poxvirus (spv) and goat poxvirus (gpv) are the most serious poxviruses of ruminants. in this study, we analysed the g-protein-coupled chemokine receptor (gpcr) genes of lsdv isolates from cattle and water buffalo (bubalus bubalis) in egypt during the summer of 2011. multiple alignments of the nucleotide sequences revealed that the water buffalo lsdv isolate differed from the cattle isolate at four nucleotide positions, and both isolates had nine nucleotide ... | 2016 | 25754131 |
| involvement of the nervous system following experimental infection with pasteurella multocida b:2 in buffalo (bubalus bubalis): a clinicopathological study. | haemorrhagic septicaemia (hs) is an acute, fatal, septicaemic disease of cattle and buffaloes caused by one of two specific serotypes of pasteurella multocida b:2 and e:2 in asian and african, respectively. it is well known that hs affect mainly the respiratory and digestive tracts. however, involvement of the nervous system in pathogenesis of hs has been reported in previous studies without details. in this study, nine buffalo calves of 8 months old were distributed into three groups. animals o ... | 2016 | 26850845 |
| seroprevalence of foot-and-mouth disease in susceptible wildlife in israel. | foot-and-mouth disease (fmd) epidemics recur in israel almost every year. wild even-toed ungulates are seldom affected during these epidemics. the seroprevalence of fmd in wild ungulates during 2000 and 2005-2013 was estimated using anti-non-structural proteins elisa. overall, 209 samples were tested, comprising sera of 120 wild boar (sus scrofa lybicus), 64 mountain gazelles (gazella gazella gazella), 6 water buffaloes (bubalus bubalis), and 19 persian fallow deer (dama dama mesopotamica). none ... | 2016 | 27200364 |
| caspase-9 inhibitor z-lehd-fmk enhances the yield of in vitro produced buffalo (bubalus bubalis) pre-implantation embryos and alters cellular stress response. | the present investigation was done to study the effect of caspase-9 inhibitor z-lehd-fmk, on in vitro produced buffalo embryos. z-lehd-fmk is a cell-permeable, competitive and irreversible inhibitor of enzyme caspase-9, which helps in cell survival. buffalo ovaries were collected from slaughterhouse and the oocytes were subjected to in vitro maturation (ivm), in vitro fertilization (ivf) and in vitro culture (ivc). the culture medium was supplemented with z-lehd-fmk at different concentrations i ... | 2016 | 26850530 |
| comparative proteomic analysis of mature and immature oocytes of the swamp buffalo (bubalus bubalis). | maternal protein components change markedly during mammalian oogenesis. many of these proteins have yet to be characterized and verified. in this study, a proteomics approach was used to evaluate changes in proteins during oogenesis in the swamp buffalo (bubalus bubalis). proteins from 500 immature oocytes and 500 in vitro matured oocytes were subjected to two-dimensional electrophoresis, and more than 400 spots were detected. image analysis indicated that 17 proteins were differentially express ... | 2016 | 26784167 |
| heat stress and antioxidant enzyme activity in bubaline (bubalus bubalis) oocytes during in vitro maturation. | in vitro environments like heat stress usually increase the production of reactive oxygen species in bubaline oocytes which have been implicated as one of the major causes for reduced developmental competence. oocytes during meiotic maturation are sensitive to oxidative stress, and heat stress accelerates cellular metabolism, resulting in the higher production of free radicals. therefore, the aim of present work was to assess the impact of heat stress during meiotic maturation on bubaline cumulu ... | 2016 | 26781547 |
| the rediscovery of malaria parasites of ungulates. | over a hundred years since their first description in 1913, the sparsely described malaria parasites (genus plasmodium) of ungulates have been rediscovered using molecular typing techniques. in the span of weeks, three studies have appeared describing the genetic characterization and phylogenetic analyses of malaria parasites from african antelope (cephalophus spp.) and goat (capra aegagrus hircus), asian water buffalo (bubalus bubalis), and north american white-tailed deer (odocoileus virginian ... | 2016 | 27444556 |
| evaluation of leptin receptor expression on buffalo leukocytes. | experimental evidences support a direct role for leptin in immunity. besides controlling food intake and energy expenditure, leptin was reported to be involved in the regulation of the immune system in ruminants. the aim of this work was to highlight the expression of leptin receptor (lepr) on bubalus bubalis immune cells using a multi-approach assessment: flow cytometry, confocal microscopy and gene expression analysis. flow cytometric analysis of lepr expression showed that peripheral blood mo ... | 2016 | 27436440 |
| production of hand-made cloned buffalo (bubalus bubalis) embryos from non-viable somatic cells. | use of non-viable somatic cells for hand-made cloning (hmc) can enable production of cloned animals from tissues obtained from elite or endangered dead animals. buffalo skin fibroblast cells were rendered non-viable by heat treatment and used for hmc. although fusion (93.6 ± 1.72 vs 67.1 ± 2.83%) and cleavage (90.3 ± 1.79 vs 65.8 ± 1.56%) rate was lower (p < 0.001) than that for controls, blastocysts could be successfully produced. however, blastocyst rate (34.1 ± 2.43 vs 6.9 ± 2.18%, p < 0.001) ... | 2016 | 27422686 |
| synonymous single nucleotide polymorphisms in the mc4r gene that are significantly associated with milk production traits in water buffaloes. | melanocortin-4 receptor (mc4r) is associated with feed intake, growth, fatness, and carcass composition in many domestic animals. the aim of this study was to evaluate the association of single nucleotide polymorphisms (snps) in mc4r with milk production traits in water buffalo. eight snps were identified by direct polymerase chain reaction sequencing of samples from 18 buffaloes. snps were then genotyped using the matrix-assisted laser desorption/ionization time of flight mass spectrometry (mal ... | 2016 | 27420995 |
| computational studies on receptor-ligand interactions between novel buffalo (bubalus bubalis) nucleotide-binding oligomerization domain-containing protein 2 (nod2) variants and muramyl dipeptide (mdp). | nucleotide binding and oligomerization domain 2 (nod2), a member of intracellular nod-like receptors (nlrs) family, recognizes the bacterial peptidoglycan, muramyl dipeptide (mdp) and initiates host immune response. the precise ligand recognition mechanism of nod2 has remained elusive, although studies have suggested leucine rich repeat (lrr) region of nod2 as the possible binding site of mdp. in this study, we identified multiple transcripts of nod2 gene in buffalo (bunod2) and at least five lr ... | 2016 | 26897084 |
| identification of polymorphism in fatty acid binding protein 3 (fabp3) gene and its association with milk fat traits in riverine buffalo (bubalus bubalis). | the fatty acid binding protein 3 (fabp3) gene, known to be associated with fat percentage of milk and meat in bovines, was screened among swamp and riverine buffaloes for polymorphism detection and further association with milk fat contents. an snp g.307c > t was identified in the intron 2 (+53 exon 2) region of fabp3 gene of indian buffaloes. the snp identified was genotyped in 692 animals belonging to 15 riverine, swamp and hybrid (riverine × swamp) buffalo populations of diverse phenotypes an ... | 2016 | 26894500 |
| isolation of bacteria in semen and evaluation of antibiotics in extender for cryopreservation of buffalo (bubalus bubalis) bull spermatozoa. | this study was designed to investigate the occurrence of bacterial species in water buffalo semen at the time of collection/processing and to assess the efficacy of some selected antibiotics (gtls; gentamycin, tylosin and linco-spectin or sp; streptomycin and penicillin) in cryodiluent on bacterial control and quality including in vivo fertility of buffalo spermatozoa. for this purpose, four experiments were conducted. in experiment 1, a total of 11 bacterial species were isolated from buffalo e ... | 2016 | 26892806 |
| effect of egg yolk powder on freezability of murrah buffalo (bubalus bubalis) semen. | the aim of this study was to investigate the effect of commercial egg yolk powder as an alternative to fresh egg yolk on freezability of murrah buffalo semen. | 2016 | 27397983 |
| modulatory role of leptin on ovarian functions in water buffalo (bubalus bubalis). | the aim of the present study was to demonstrate the modulatory role of leptin on bubaline granulosa cells (gcs) and luteal cells (lcs) functions using an in vitro cell culture system and to establish a cross talk between leptin and insulin-like growth factor-1 (igf-1). gcs were collected from group iv follicles (>13 mm size) and lcs from mid-luteal phase corpus luteum and were grown in serum-containing media supplemented with leptin at three different dose rates (0.1, 1, and 10 ng/ml) and time d ... | 2016 | 27381558 |
| efficiency of beetle (dendroides canadensis) recombinant antifreeze protein for buffalo semen freezability and fertility. | overwintering larvae of the beetle dendroides canadensis produce potent antifreeze proteins to inhibit inoculative freezing and promote supercooling. we hypothesized that addition of dendroides canadensis recombinant antifreeze proteins (dafps) in the extender will improve the quality and fertility of cryopreserved nili-ravi buffalo (bubalus bubalis) sperm. the study was divided into two parts: (1) evaluation of the effect of dafps on the quality of frozen-thawed buffalo bull sperm and (2) exami ... | 2016 | 27349137 |
| serological investigation of leptospira infection and its circulation in one intensive-type water buffalo farm in the philippines. | water buffalo is an indispensable livestock in the philippines. leptospirosis is a serious zoonosis that can be fatal to humans and cause reproductive problems in livestock. leptospirosis has been reported in some countries where water buffaloes are commercially raised, highlighting the leptospira prevalence in this farming system, but information on leptospirosis in water buffalo farms in the philippines is limited. in this study, we collected blood samples from rats (n = 21), and water buffalo ... | 2016 | 27348885 |
| correction: comparative pharmacokinetics of ceftiofur hydrochloride and ceftiofur sodium after administration to water buffalo (bubalus bubalis). | 2016 | 27347830 | |
| semen quality parameters as fertility predictors of water buffalo bull spermatozoa during low-breeding season. | the present study was carried out to assess various postthaw semen quality parameters for the prediction of fertility in buffalo bull during low-breeding season. semen (30 ejaculates) was collected from five adult buffalo bulls with artificial vagina (42 °c). sperm motility parameters, velocity distribution, motion kinematics, and subpopulations were analyzed by computer-aided sperm motion analyzer (casa). moreover, sperm visual motility, supravital plasma membrane integrity, viability/acrosome ... | 2016 | 27321805 |
| clinical and ultrasonographic observations of functional and mechanical intestinal obstruction in buffaloes (bubalus bubalis). | this study was designed for clinical and laboratory evaluation of intestinal obstruction (io) in buffaloes (bubalus bubalis) with special emphasis on the diagnostic value of ultrasonographic findings. | 2016 | 27284223 |
| seasonal and ageing-depending changes of aquaporins 1 and 9 expression in the genital tract of buffalo bulls (bubalus bubalis). | the presence of aquaporins 1 (aqp1) and 9 (aqp9), integral membrane water channels that facilitate rapid passive movement of water and solutes, was immunohistochemically detected in the excurrent ducts collected from sexually mature buffalo bulls of proven fertility during the mating (late autumn-winter) and non-mating (late spring to the beginning of autumn) seasons. furthermore, the research was performed also on the epididymal cauda of a senile buffalo bull with inactive testis. aquaporins 1 ... | 2016 | 27260501 |
| igf1 stabilizes sperm membrane proteins to reduce cryoinjury and maintain post-thaw sperm motility in buffalo (bubalus bubalis) spermatozoa. | insulin like growth factor 1 (igf1) in the seminal plasma is reported to improve sperm motility by reducing oxidative stress. the present study was conducted to assess the effect of addition of igf1 on sperm function and protein composition during cryopreservation process. semen samples were collected from six murrah buffaloes (2 ejaculates from each animal) and diluted (80 million/ml) in tris egg yolk extender and divided into control, t1, t2 and t3, groups supplemented with 0, 50, 100 and 150 ... | 2016 | 27256665 |
| use of peripheral blood for production of buffalo (bubalus bubalis) embryos by handmade cloning. | buffalo embryos were produced by handmade cloning using peripheral blood-derived lymphocytes as donor cells. although the blastocyst rate was lower (p < 0.01) for lymphocyte- than control skin fibroblast-derived embryos (6.6 ± 0.84% vs. 31.15 ± 2.97%), the total cell number (152.6 ± 23.06 vs. 160.1 ± 13.25) and apoptotic index (6.54 ± 0.95 vs. 8.45 ± 1.32) were similar. the global level of h3k9ac was higher (p < 0.05) in lymphocyte- than that in skin-derived blastocysts; whereas in ivf blastocys ... | 2016 | 27242179 |
| genetic and genomic dissection of prolactin revealed potential association with milk production traits in riverine buffalo. | milk yield and quality has been a major selection criterion for genetic improvement in livestock species. role of prolactin gene in determining milk quality in terms of protein profile, lactose, lipids and other imperative macromolecules is very important. in this context, genetic profiling of prolactin gene in riverine buffalo of pakistan was performed and potential genetic markers were identified illustrating worth of this gene in marker-assisted selection of superior dairy buffaloes. series o ... | 2016 | 27240674 |
| comparative pharmacokinetics of ceftiofur hydrochloride and ceftiofur sodium after administration to water buffalo (bubalus bubalis). | objective to evaluate pharmacokinetics and bioavailability after administration of ceftiofur hydrochloride and ceftiofur sodium to water buffalo (bubalus bubalis). animals 5 healthy adult water buffalo (3 males and 2 nonlactating females). procedures all animals received a dose (2.2 mg/kg) of 3 ceftiofur products (2 commercially available suspensions of ceftiofur hydrochloride [cef1 and cef2, im] and ceftiofur sodium [cef3, iv]). blood samples were collected for up to 196 hours. concentrations o ... | 2016 | 27227504 |
| utility of ultrasonography for diagnosis of superficial swellings in buffalo (bubalus bubalis). | we studied 72 buffalo with superficial swellings in the head (n=4), neck (n=5), chest wall (n=4), abdominal wall (n=28), limbs (n=16), gluteal region (n=8), perineal region (n=6) and udder (n=1). ultrasonographically, the swellings varied according to type, duration, content and location. the clinical use of ultrasound to assess these superficial swellings allowed diagnosis of abscesses (n=21), hematomas (n=11), hernias (n=17), bursitis (n=13), urethral diverticula (n=6) and tumors (n=4). ultras ... | 2016 | 27181085 |
| saliva ferning, an unorthodox estrus detection method in water buffaloes (bubalus bubalis). | estrus detection is a major problem in buffalo husbandry because of inconsistent expression of estrous signs at different seasons, and a high prevalence of the silent heat and postpartum anestrus in this species. around 50% of the estrus events in buffaloes are currently undetected in the field conditions, resulting in a huge economic loss. although the cervicovaginal fluid fern patterns confirm the estrus for a breeding decision, the fluid discharge is absent during the silent-heat condition. t ... | 2016 | 27181055 |
| impact of buserelin acetate or hcg administration on the day of first artificial insemination on subsequent luteal profile and conception rate in murrah buffalo (bubalus bubalis). | this study was designed to investigate the impact of buserelin acetate (ba) or human chorionic gonadotropin (hcg) administration on the day of first artificial insemination (ai) on subsequent luteal profile (diameter of corpus luteum (cl) and plasma progesterone) and conception rate in murrah buffalo. the present experiment was carried out at two locations in 117 buffalo that were oestrus-synchronized using cloprostenol (500 μg) administered (i.m.) 11 days apart followed by ai during standing oe ... | 2016 | 27170495 |
| strategies to overcome seasonal anestrus in water buffalo. | reproductive seasonality in buffalo (bubalus bubalis) is characterized by behavioral, endocrine, and reproductive changes that occur over distinct periods of the year. during the nonbreeding season (spring and summer), the greater light-dark ratio (long days) suppresses estrus behavior and the occurrence of ovulation. anestrous buffaloes have insufficient pulsatile of lh to support the final stages of follicular development, and subsequently, estrus behavior and ovulation do not occur, limiting ... | 2016 | 27157389 |
| effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (bubalus bubalis) bull spermatozoa. | the effects of equilibration times (e1, 2 h; e2, 4 h; e3, 6 h), freezing rates (fr1, manual, 5 cm above liquid nitrogen (ln2 ) for 10 min, plunging in ln2 ; fr2, programmable ultra-fast, holding at +4 °c for 2 min, from 4 to -10 °c at -10 °c/min, from -10 to -20 °c at -15 °c/min, from -20 to -120 °c at -60 °c/min, holding at -120 °c for 30 sec, plunging in ln2 ), and thawing rates (t1, 37 °c for 30 sec; t2, 50 °c for 15 sec; t3, 70 °c for 7 sec) were evaluated on quality of buffalo bull spermato ... | 2016 | 27153390 |
| embryonic and fetal mortality in river buffalo (bubalus bubalis). | river buffalo are able to adapt to diverse climatic zones and are widespread globally. the resource use efficiency of buffalo is highly relevant in a resource-constrained world and the increasing requirement to produce more food. buffalo clearly have an important role in meeting the growing demand for animal protein. in the mediterranean and higher latitudes, buffalo show annual cycles of ovarian activity, embryonic development, and pregnancy rate. in buffalo, the cl starts to develop early in t ... | 2016 | 27142486 |
| water buffalo (bubalus bubalis) as a spontaneous animal model of vitiligo. | vitiligo is a multifactorial acquired depigmenting disorder. recent insights into the molecular mechanisms driving the gradual destruction of melanocytes in vitiligo will likely lead to the discovery of novel therapies, which need to be evaluated in animal models that closely recapitulate the pathogenesis of human vitiligo. in humans, vitiligo is characterized by a spontaneous loss of functional melanocytes from the epidermis, but most animal models of vitiligo are either inducible or geneticall ... | 2016 | 27124831 |
| microorganisms in the rumen and reticulum of buffalo (bubalus bubalis) fed two different feeding systems. | the community of microorganisms in the rumen and reticulum is influenced by feeding as well as the species and geographical distribution of ruminant animals. bacteria, methanogenic archaea and ciliate protozoa existing in the rumen and reticulum were evaluated by real-time polymerase chain reaction and light microscopy in buffalo in two feeding systems, grazing and feedlot. | 2016 | 27118203 |
| flow cytometric and near-infrared raman spectroscopic investigation of quality in stained, sorted, and frozen-thawed buffalo sperm. | flow cytometry and laser tweezers raman spectroscopy have been used to investigate nili-ravi buffalo (bubalus bubalis) sperm from different samples (fresh, stained, sorted and frozen-thawed) of the flow-sorting process to optimize sperm sex sorting procedures. during the sorting and freezing-thawing processes, the two detection methods both indicated there were differences in mitochondrial activity and membrane integrity. moreover, a dispersive-type nir (near infrared reflection) use of the rama ... | 2016 | 27095613 |
| anti-mullerian hormone (amh) concentration in follicular fluid and mrna expression of amh receptor type ii and lh receptor in granulosa cells as predictive markers of good buffalo (bubalus bubalis) donors. | high individual variability in follicular recruitment and hence in the number of embryos produced is a major factor limiting the application of reproductive technologies in buffalo. therefore, the identification of reliable markers to select embryo donors is critical to enroll buffaloes in embryo production programs. better understanding of factors involved in follicular growth is also necessary to improve the response to superovulation in this species. the aim of this work was thus to determine ... | 2016 | 27087535 |
| understanding tenderness variability and ageing changes in buffalo meat: biochemical, ultrastructural and proteome characterization. | understanding of biological impact of proteome profile on meat quality is vital for developing different approaches to improve meat quality. present study was conducted to unravel the differences in biochemical, ultrastructural and proteome profile of longissimus dorsi muscle between buffaloes (bubalus bubalis) of different age groups (young v. old). higher (p<0.05) myofibrillar and total protein extractability, muscle fibre diameter, and warner-bratzler shear force (wbsf) values was observed in ... | 2016 | 27076348 |
| ultrastructural changes in the sublingual salivary gland of prenatal buffalo (bubalus bubalis). | the present study was aimed to elucidate ultrastructural changes in the development of sublingual salivary gland of buffalo during prenatal life. | 2016 | 27057120 |
| effect of incubation on freezability of cholesterol-loaded cyclodextrin treated buffalo (bubalus bubalis) spermatozoa. | the aim of this study was to investigate the effect of incubation on freezability of cholesterol loaded cyclodextrin (clc) treated buffalo spermatozoa. | 2016 | 27051205 |
| effect of age, sex and physiological stages on hematological indices of banni buffalo (bubalus bubalis). | to determine the physiological baseline values for hematological indices of banni buffalo (bubalus bubalis) as well as to assess their alteration due to age, sex and physiological stages. | 2016 | 27051182 |
| corrigendum to "investigation into omocysteine, vitamin e and malondialdehyde as indicators of successful artificial insemination in synchronized buffalo cows (bubalus bubalis)" [res. vet. sci. volume 104, february 2016, pages 100-105]. | 2016 | 27495365 | |
| random regression models to estimate genetic parameters for weights in murrah buffaloes. | this article reports genetic analysis of the weight at different ages of murrah water buffaloes, using random regression models (rrm). models ranging from third to sixth order polynomial were used to describe direct genetic and animal permanent environmental effects. contemporary group was included as a fixed effect, and a cubic polynomial was used to model the mean curve of the population. the residual was modeled considering a log-linear function. two models were selected for study of genetic ... | 2016 | 28004872 |
| efficiency of uterine fluid cytology in the diagnosis of subclinical endometritis in the water buffalo (bubalus bubalis). | this study compared endometrial cytology vis-a-vis uterine fluid cytology for assessment of uterine health in clinically normal and subclinical endometritis (se)-affected buffaloes. uterine fluid samples and endometrial samples were collected from the buffaloes (n = 38) at oestrus using blue sheath and cytobrush, respectively. the smears were stained with field stain for 3 minutes, and a minimum of 400 cells were counted in each smear for determination of the percentage of polymorphonuclear (pmn ... | 2016 | 27966806 |
| programmable fast-freezing method improves the post-thaw motion dynamics, integrities of plasmalemma, mitochondrial transmembrane, dna and, acrosome, and in vivo fertility of water buffalo (bubalus bubalis) spermatozoa. | the effects of freezing methods (fr1, nonprogrammable/static, 5 cm above liquid nitrogen [ln2 ] for 10 min, plunging in ln2 ; fr2, programmable medium, +4°c to -15°c at 3°c min(-1) , from -15 to -80°c at 10°c min(-1) and final holding for 1 min at -80°c, plunging in ln2 ; fr3, programmable fast, from initial holding at +4°c for 2 min, from +4°c to -20°c at 10°c min(-1) , from -20°c to -100°c at 30°c min(-1) , final holding for 1 min at -100°c and plunging in ln2 ) were assessed on post-thaw in v ... | 2016 | 27813131 |
| identification of four snps in lhb gene and their associations with sperm qualities of chinese buffaloes. | luteinizing hormone beta polypeptide (lhb) gene has been considered important for sexual behavior and has associations with sperm quality. in this study, four snps (g.276 t>c, g.377a>c, g.401t>c, and g.412a>g) were detected in the lhb gene of 165 water buffaloes by direct sequencing and identification of overlap peaks, each of which was associated with at least one sperm quality trait of ejaculate volume, sperm concentration, post-thaw sperm motilities, and sperm abnormalities by chi-square anal ... | 2016 | 27854152 |
| effect of terminalia arjuna bark powder on some diagnostic enzymes in buffalo (bubalus bubalis) ingesting arsenic contaminated water and fodder. | the study investigated the effect of terminalia arjuna bark powder on some diagnostic enzymes related to hepatic and muscle function in buffaloes ingesting arsenic contaminated water and fodder in an arsenic affected area. | 2016 | 27847431 |
| seasonal variations of italian mediterranean buffalo (bubalus bubalis) mozzarella cheese quality. | water buffalo mozzarella cheese is more appreciated in the summer, but milk production is higher during wintertime, as water buffalo are seasonally polyoestrous animals. the aim of the study reported in this research communication was to evaluate the effect of the reversing of the calving period on mozzarella cheese composition and quality traits. ten batches of mozzarella cheeses produced during the summer and winter periods were analysed for ph, colour, chemical composition. seasonal differenc ... | 2016 | 27845021 |
| freezability of water buffalo bull (bubalus bubalis) spermatozoa is improved with the addition of curcumin (diferuoyl methane) in semen extender. | effects of curcumin as antioxidant in extender were evaluated on freezability of buffalo spermatozoa. semen from each of the five bulls (n = 3 replicates, six ejaculates/bull, a total of 30 ejaculates) was diluted in tris-citric acid extender containing curcumin (0.5, 1.0, 1.5 or 2.0 mm) or control. at pre-freezing and post-thawing, total antioxidant contents (μm/l) and lipid peroxidation levels (μm/ml) were higher (p < .05) and lower (p < .05) respectively, with 1.5 and 2.0 mm compared to 0.5 a ... | 2016 | 27709643 |
| polymorphisms in the dgat1 gene in buffaloes (bubalus bubalis) in the amazon. | water buffaloes (bubalus bubalis) are quite well adapted to climatic conditions in the amazon, and in this biome, they are noted for the considerable amount of meat and milk they produce and how hard they are able to work. because of a lack of research dedicated to improving the rearing of buffaloes in the amazon, the objective of this study was to genetically characterize the murrah and mediterranean breeds, as well as a mixed-breed population, based on polymorphisms in the diacylglycerol o-acy ... | 2016 | 27706739 |
| effect of sex of embryo on developmental competence, epigenetic status, and gene expression in buffalo (bubalus bubalis) embryos produced by hand-made cloning. | buffalo embryos were produced by hand-made cloning using skin fibroblasts from male and female buffaloes (n = 4 each) as donor cells for examining the effect of sex. although the rate of blastocyst formation (43.8% ± 1.31% vs. 42.2% ± 1.22%) was similar, the total cell number (333 ± 10.4 vs. 270 ± 10.9) was higher (p < 0.05) whereas the apoptotic index (6.39 ± 0.25 vs. 8.52 ± 0.38) was lower (p < 0.05) for male than for female blastocysts. in the blastocysts, the global level of h3k18ac was foun ... | 2016 | 27696910 |
| complete genome sequence of a myoviridae bacteriophage infecting salmonella enterica serovar typhimurium. | the bacteriophage 118970_sal3 was isolated from water buffalo feces in southern italy, exhibiting lytic activity against salmonella enterica serovar typhimurium. this bacteriophage belongs to the myoviridae family and has a 39,464-bp double-stranded dna (ds-dna) genome containing 53 coding sequences (cdss). | 2016 | 27688333 |
| nutrient utilisation, growth performance and blood metabolites in murrah buffalo calves (bubalus bubalis) divergently selected for residual feed intake. | the aim of this study was to evaluate differences in efficiency of feed utilisation between buffalo calves with low and high residual feed intake (rfi) by comparing feed intake, nutrient digestibility, growth traits and blood metabolites. eighteen male murrah buffalo calves (aged 4-6 months; 70 ± 1.0 kg body weight) were fed ad libitum with a total mixed ration for 120 d. based on linear regression models involving dry matter intake (dmi), average daily gain (adg) and mid-test metabolic body siz ... | 2016 | 27666680 |
| developmental competence and expression profile of genes in buffalo (bubalus bubalis) oocytes and embryos collected under different environmental stress. | the study examined the effects of different environmental stress on developmental competence and the relative abundance (ra) of various gene transcripts in oocytes and embryos of buffalo. oocytes collected during cold period (cp) and hot period (hp) were matured, fertilized and cultured in vitro to blastocyst hatching stage. the mrna expression patterns of genes implicated in developmental competence (oct-4, igf-2r and gdf-9), heat shock (hsp-70.1), oxidative stress (mnsod), metabolism (glut-1), ... | 2016 | 27650183 |
| a comparative analysis of sperm selection procedures prior to cryopreservation for nili-ravi buffalo bull (bubalus bubalis) semen-: assessment of its impact on post-thaw sperm functional quality. | sperm selection techniques have been developed to get sperm suspensions enriched in motile and functional cells. studies show that selection before cryopreservation improves post-thaw quality of cryopreserved sperm but information on buffalo bull sperm is scarce. the study was aimed to 1) perform a comparative analysis of sperm selection procedures; swim-up (su), sephadex™-g15 filtration (s-g15) or glass wool filtration (gwf) for total and motile cell recovery, 2) to assess the impact of sperm s ... | 2016 | 27616354 |