Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the construct validity of passive-aggressive personality disorder. | although passive aggressive personality disorder (papd) plays an important role in many theories of personality pathology, it was consigned to the appendix of the fourth edition of the dsm. the scientific basis of this decision has been questioned, but several controversies persist regarding papd, including its structure, content validity, overlap with other pds, and relations to validating variables such as personality traits, childhood experiences, and clinically relevant correlates. this stud ... | 0 | 19821648 |
| on the value of homogeneous constructs for construct validation, theory testing, and the description of psychopathology. | the authors argue for a significant shift in how clinical psychology researchers conduct construct validation and theory validation tests. they argue that sound theory and validation tests can best be conducted on measures of unidimensional or homogeneous constructs. hierarchical organizations of such constructs are useful descriptively and theoretically, but higher order composites do not refer to definable psychological processes. application of this perspective to the approach of the diagnost ... | 0 | 19719340 |
| pathogenesis of kidney disease in systemic lupus erythematosus. | a combination of systemic autoimmunity and tissue response to immune injury underlie renal involvement in lupus erythematosus. in this review, we discuss recent literature investigating pathogenetic mechanisms of lupus glomerulonephritis. | 0 | 19584729 |
| factor structure of the children's perception of interparental conflict scale for studies of youths with externalizing behavior problems. | the assessment of children's perception of marital conflict is an important area of research related to family relations, children's cognitions about self, and the development of psychopathology. the leading instrument in this domain is the children's perception of interparental conflict scale (cpic; j. h. grych, m. seid, & f. d. fincham, 1992). it has 48 items organized into 9 conceptual designed subscales, with reports of 3-factor higher order structure to the 9 subscales. however, the 3-facto ... | 0 | 19719356 |
| modified therapeutic communities and adherence to traditional elements. | traditional therapeutic communities (tcs) are characterized by confrontational group therapy, treatment phases, a tenure-based resident hierarchy, and long-term residential care. many tcs have modified the structure and intensity of the traditional model, tailored services for specific client populations, and hired more professionally trained staff. this study examines the extent to which modified tcs are able to retain the underlying core technology of the tc. using data from a nationally repre ... | 0 | 19999681 |
| reasons for abstaining or limiting drinking: a developmental perspective. | the cross-sectional and longitudinal relations between reasons for abstaining or limiting drinking (rald) and abstention were examined in a 16-year longitudinal study (n = 489) of college students with and without a family history of alcohol problems. results indicated that rald based upon upbringing or religiosity were associated with increased rates of abstention, whereas rald based upon perceived or experienced negative consequences of drinking were associated with lower rates of abstention a ... | 0 | 19769427 |
| why i hit him: women's reasons for intimate partner violence. | this study examines motives for intimate partner violence (ipv) among a community sample of 412 women who used ipv against male partners. a "motives and reasons for ipv scale" is proposed, and exploratory factor analyses identified five factors: expression of negative emotions, self-defense, control, jealousy, and tough guise. to our knowledge, the study is the first to investigate the relationship between women's motives for ipv and their perpetration of physical, psychological, and sexual aggr ... | 0 | 21072136 |
| two-part factor mixture modeling: application to an aggressive behavior measurement instrument. | this study introduces a two-part factor mixture model as an alternative analysis approach to modeling data where strong floor effects and unobserved population heterogeneity exist in the measured items. as the names suggests, a two-part factor mixture model combines a two-part model, which addresses the problem of strong floor effects by decomposing the data into dichotomous and continuous response components, with a factor mixture model, which explores unobserved heterogeneity in a population b ... | 0 | 20717486 |
| central sensitization: a generator of pain hypersensitivity by central neural plasticity. | central sensitization represents an enhancement in the function of neurons and circuits in nociceptive pathways caused by increases in membrane excitability and synaptic efficacy as well as to reduced inhibition and is a manifestation of the remarkable plasticity of the somatosensory nervous system in response to activity, inflammation, and neural injury. the net effect of central sensitization is to recruit previously subthreshold synaptic inputs to nociceptive neurons, generating an increased ... | 0 | 19712899 |
| psychometric evaluation of the health information orientation scale: a brief measure for assessing health information engagement and apprehension. | the health information orientation scale (hios) was developed from a need to briefly assess information orientation in a health context and underlying reasons for information seeking or avoidance. using data from a larger longitudinal study of informal cancer caregivers, this study examines psychometric properties of the hios, including confirmatory factor analysis (cfa), reliability and construct validity through associations with information competence, coping and distress. cfa supported two c ... | 0 | 19687109 |
| the role of flow injection analysis within the framework of an automated laboratory. | flow injection analysis (fia) was invented at roughly the same time by two quite dissimilar research groups [1,2]. fia was patented by both groups in 1974; a year also marked by the publication of the first book on automatic chemical analysis [3]. this book was a major undertaking for its authors and it is hoped that it has added to the knowledge of those analysts attempting to automate their work or to increase the level of computerization/automation and thus reduce staffing commitments. this r ... | 0 | 18925262 |
| the kinetics of oral hyposensitization to a protein antigen are determined by immune status and the timing, dose and frequency of antigen administration. | we have investigated the immunological consequences of feeding a protein antigen to previously immunized animals. balb/c mice were systemically primed with ovalbumin (ova) in complete freund's adjuvant (cfa) and fed with high (10 mg/g body weight), medium (1 mg/g body weight) or low (1 microgram/g body weight) doses of ova once (day 1, 7 or 14) or sequentially for 5 days (days 1-5, 7-11, 14-18). the specific igg antibody response was suppressed only by early feeds of high-dose ova (days 1-5). me ... | 0 | 2759662 |
| immunological responsiveness of neonatal a/j mice to isotypic determinants of syngeneic ige. | we have previously shown that adult a/j mice produce high titers of anti-ige with isotypic or idiotypic specificities in response to challenge with a conjugate of klh with syngeneic monoclonal ige. thus, b cells that can synthesize anti-ige are present in the mice. adult mice are unresponsive to unconjugated ige in cfa, suggesting that tolerance exists at the level of t cells. the present study shows that neonatal mice produce anti-ige antibodies in response to unconjugated ige in cfa, but that ... | 0 | 3411291 |
| suppression of an established dth response to ovalbumin in mice by feeding antigen after immunization. | experiments were designed to examine whether systemic delayed-type hypersensitivity responses (dth) to ovalbumin (ova) can be suppressed when antigen is fed after immunization, and to investigate the immunological mechanisms involved. a single 25 mg feed of ova given 7 or 14 days after immunization with ova in complete freund's adjuvant (cfa) suppressed the dth response of bdf1 mice, but had no significant effect on the serum igg antibody response. dth suppression was greatest when antigen was f ... | 0 | 3384449 |
| inhibition of an ige response by secondary b cells of a different isotype. | we found that the synthesis of ige anti-ars antibodies is strongly inhibited by the presence of secondary non-ige-producing cells that are specific for the ars hapten. such b cells can be induced by inoculation of a protein-ars conjugate in cfa. the effect is seen after inoculation of ova-ars in cfa followed by klh-ars in alum, or, more convincingly, after adoptive transfer of b cells induced by antigen in cfa. dose-response data indicated that inhibition can be effected by b cells containing no ... | 0 | 3491175 |
| delayed type hypersensitivity (dth) to type ii collagen (cii) in dba-1 mice. | sensitization of dba-1 mice with type ii collagen (cii) in complete freund's adjuvant can cause polyarthritis. a possible link between cii-induced arthritis and delayed type hypersensitivity (dth) has been suggested, so we decided to investigate the susceptibility of dba-1 mice to cii induced dth reactions. the mice were primed with a dose of 10 micrograms cii i.p. 4 days before challenging with 40 micrograms cii in the ear. swelling was measured 48 h later and was found to be reproducible. resp ... | 0 | 3491709 |
| an item response theory/confirmatory factor analysis of the autobiographical memory test. | the autobiographical memory test (amt) is used to assess the degree of specificity of autobiographical memory. the amt usually contains cue words of both positive and negative valence, but it is unclear whether these valences form separate factors or not. accordingly, confirmatory factor analysis assessed whether the amt measures one overall factor, or whether different cue types are related to different factors. results were consistent across three datasets (n = 333, n = 405, and n = 336). a on ... | 0 | 19585345 |
| anticipating problem drinking risk from preschoolers' antisocial behavior: evidence for a common delinquency-related diathesis model. | early first drinking (efd) experiences predict later alcohol problems. however, the longitudinal pathway from early childhood leading to efd has not been well delineated. based on documented links between drinking behaviors and chronic antisocial behaviors, this article tests a common diathesis model in which precursive patterns of aggression and delinquent behavior-from preschool onward-anticipate efd. | 0 | 19564798 |
| are there stable factors in preadolescent girls' externalizing behaviors? | relatively little is known about the factor structure of disruptive behavior among preadolescent girls. the present study reports on exploratory and confirmatory factor analyses of disruptive girl behavior over four successive data waves as rated by parents and teachers in a large, representative community sample of girls (n = 2,451). five factors were identified from parent ratings (oppositional behavior/conduct problems, inattention, hyperactivity/impulsivity, relational aggression, and callou ... | 0 | 19437114 |
| synergistic interaction of macrophages and lymphocytes in antigen-induced transformation of lymphocytes. | the role of macrophages and lymphocytes in antigen-induced transformation of lymphocytes has been investigated. lymphocytes and macrophages were obtained from inbred strain 13 guinea pigs which were either unimmunized or immunized with complete freund's adjuvant (cfa) or tetanus toxoid in cfa. the transformation response to ppd or tetanus toxoid was assayed by tritiated thymidine incorporation. addition of macrophages to immune lymphocytes significantly increased their response to purified prote ... | 1970 | 4994445 |
| potentiating effect of freund's adjuvant on interferon production by endotoxin or poly ri*poly rc. | intraperitoneal injection of mice with mineral oil, incomplete (ifa) or complete freund's adjuvant (cfa) increased the interferon response to endotoxin or (poly ri)*(poly rc) administered intravenously 2 days later. after endotoxin administration, circulating interferon titers were increased at several different times of sampling and with a variety of endotoxin dosages. when injection of endotoxin was delayed until 6 to 8 days after the administration of ifa or cfa, interferon production was mar ... | 1970 | 16557802 |
| the traditional village midwives. | the training of the village midwife, (a position not to be confused with official or council midwives who work in an established dispensary), in western cameroon, is complicated by traditional notions of childbirth and pregnancy. the need for midwifery with a medical background is obvious from the following statistics, gleaned from 100 reported pregnancies: of the 100 pregnancies reported, there were 7 abortions, 24 stillbirths before day 10 of life, and 69 surviving infants. in a culture where ... | 1970 | 12278371 |
| heterogeneity of the cellular immune response. ii. the role of adjuvant, lymphocyte stimulation in cutaneous basophil hypersensitivity. | antigen-mediated stimulation of thymidine incorporation was demonstrated in lymph node cells from guinea pigs immunized with 100 microg human serum albumin in either freund's incomplete or freund's complete adjuvant. animals receiving hsa in ifa exhibited both cutaneous basophil (jones-mote) hypersensitivity and lymphocyte stimulation at 1, but not at 6 wk after immunization. significant stimulation required >/= 10 microg hsa/ml of culture. sensitization with hsa in cfa produced delayed hypersen ... | 1971 | 5316262 |
| [configural frequency analysis. 3. two or more sample cfa tests in diagnosis and differential diagnosis]. | 1971 | 5162538 | |
| [configural frequency analysis. ii. hierarchical and agglutinating cfa in clinical psychology]. | 1971 | 5162534 | |
| action of ionizing radiation on sensitive strains of escherichia coli b. | strain b(8-11) has been found to be very sensitive to postirradiation dna degradation. up to 98% of the dna is degraded at optimum doses. the amount of residual dna correlates with the retention of colony-forming ability (cfa). studies of rates of degradation as a function of dose agree with the concept that a degrading lesion causes a definite rate of degradation and that increased numbers of lesions produce proportionally faster rates. by observing the burst size of t7 phage which uses host dn ... | 1972 | 4550650 |
| interferon, antibody, and other host factors in herpes zoster. | the influence of several factors on the course of herpes zoster was studied in 151 patients. dissemination of zoster was associated with the presence of a concurrent disease, especially hodgkin's disease, and/or the use of immunosuppressive therapy. several host-immune parameters, including quantitative immunoglobulins, circulating lymphocyte counts, delayed hypersensitivity to multiple skin test antigens, and lymphocyte transformation to phytohemagglutinin did not correlate with dissemination o ... | 1972 | 5020430 |
| antigenicity of carcinogen and viral induced sarcomas in inbred and random bred guinea pigs. | a tumor specific transplantation antigen (tsta) has been detected in a methylcholanthrene (mca) induced guinea pig tumor. it was possible to induce resistance to rechallenge with the tumor by immunization with irradiated cells in cfa. in contrast, the same technique failed to detect tsta in two viral (kirsten strain mouse sarcoma virus, ki-msv) induced guinea pig tumors; these results are similar to observations made with mouse ki-msv-induced tumors. transplantation studies with these tumors in ... | 1975 | 50348 |
| immunologic activity of myelin basic protein in strain 2 and strain 13 guinea pigs. | the resistance of strain 2 guinea pigs to experimental allergic encephalomyelitis (eae) induced by inoculation with whole cns tissue in complete freund's adjuvant (cfa) has been confirmed. the resistance is even more pronounced when myelin basic protein (bp) is used in attempts to induce eae. strain 2 guinea pigs are also resistant to an immunization schedule (multiple injections with bp in ifa followed by a single injection of bp in cfa) known to induce significant levels of antibody in suscept ... | 1975 | 50359 |
| the antibody responses to myelin basic protein (bp) in lewis rats: the effects of bordetella pertussis. | a time-course study was made of the systemic humoral immune response of lewis rats to myelin basic protein (bp) as influenced by the dosage of ancillary pertussis adjuvant. peak activities were observed 5 to 7 weeks after injection. when injected proximal to bp and mycobacterium butyricum in complete freund's adjuvant (cfa), bordetella pertussis at the level of 4 billion organisms doubled the antibody-binding activity of rat sera for 125i-labeled bp as compared to activities obtained with 0, 2, ... | 1975 | 51876 |
| experimental allergic encephalomyelitis (eae) in mice. i. induction of eae with mouse spinal cord homogenate and myelin basic protein. | the condition of experimental allergic encephalomyelitis (eae) induction was investigated in several mice strains. sjl and c3h/he strains were found to be susceptible. a single immunization with mouse spinal cord, complete freund's adjuvant (cfa) and pertussis vaccine produced clinical signs of eae in sjl and c3h/he strains after 11 to 18 days. isogenic spinal cord produced eae in c3h/he strain. a single immunization with myelin basic protein from bovine spinal cord in cfa and pertussis vaccine ... | 1975 | 57253 |
| quantitative studies on cell-mediated immunity in the pleural cavity of guinea-pigs. | intrapleural injection of ppd into cfa-sensitised guinea-pigs produced a reaction having many of the characteristics of classical delayed hypersensitivity. quantitative investigations of the time course of this reaction showed that it was typified by an initial delay in the onset of increased vascular permeability, which reached its peak between 12 and 18 hr. the cellular response in the pleural cavity followed a similar pattern, attaining maximum levels between 18 and 24 hr. mononuclear cells d ... | 1975 | 178847 |
| induction of antibodies to nuclear antigens in rabbits by immunization with hydralazine-human serum albumin conjugates. | the antihypertensive drug hydralazine can induce in man a syndrome similar to spontaneous systemic lupus erythematosus (sle). the pathogenesis of this drug-induced syndrome is not understood. in this investigation, five groups of rabbits were studied: group i, 10 rabbits hyperimmunized with hydralazine conjugated to human serum albumin (hsa) in complete freund's adjuvant (cfa); group ii, four rabbits with hsa in cfa; group iii, four rabbits with cfa alone; group iv, five rabbits with hydralazine ... | 1975 | 808562 |
| [study of the possibilities of inactivating adeno-associated virus type 4]. | adeno-associated type 4 virus (aav-4) is inactivated by formalin dilutions 1 : 400, 1 : 1000 and 1 : 2000 for 24 hours at 37 degrees c. under these conditions hemogglutinins (ha) and complement-fixing antigen (cfa) are retained. the residual formalin must be neutralized with sodium bisulphite. aav-4 can also be inactivated by 3% hydrogen peroxide and ha and cfa are retained too. hydrogen peroxide may be neutralized with catalase. no inactivation of aav-4 could be achieved with lowere concentrati ... | 1975 | 1162954 |
| relationship between the response to complete freund adjuvant, phytohemagglutinin, and subsequent tumor growth in mice. | groups of inbred c3h mice selected on the basis of strong or weak ppd reactions after sensitization with complete freund adjuvant had significantly different reactions to pha. the growth rate of a methylcholanthrene-induced tumor, previously shown to elicit a cell-mediated immune response, was significantly different in these two groups of mice. the basis for the marked variation observed between members of an inbred mouse strain in response to cfa is not understood but may bear an important pot ... | 1975 | 1184209 |
| mast cell sensitizing antibody (mcsab) response in experimental candidiasis: chromatographic studies. | a single dose of the yeast-like fungus candida albicans (ca), mixed with complete freund's adjuvant (cfa), and inoculated into guinea-pig foot-pads, provoked the productions of homologous mast cell sensitizing antibodies (mcsab) present in the psii-globulin fraction only, and of agglutinins localized in the same fraction. heterologous mcsab and skin-sensitizing antibodies detectable by passive cutaneous anaphylaxis (pca) were constantly absent. these facts are in contradiction to results of prev ... | 1975 | 1098354 |
| allergic encephalomyelitis in the reputedly resistant brown norway strain of rats. | brown norway (bn) rats are much less susceptible to experimental allergic encephalomyelitis (eae) than lewis rats. nevertheless, bn rats developed severe eae, even paralysis, when immunized with rat spinal cord and carbonyl iron adjuvant. complete freund's adjuvant (cfa) was much less effective. the use of both cfa and pertussis vaccine with rat cord was moderately, but not consistently, effective. guinea pig spinal cord was weakly encephalitogenic to bn rats with all adjuvant combinations. we w ... | 1975 | 1120901 |
| suppression of antibody synthesis to dinitrophenylated bovine gamma globulin in contact-sensitized guinea pigs. | the anti-dnp response to dnp-bgg is substantially suppressed in guinea pigs sensitized to dncb. the degree of antibody suppression varies with the mode of skin sensitization and with the degree of conjugation of the challenging immunogen. suppression of the anti-hsa response was also induced by the prior injection of cfa. | 1975 | 1144454 |
| serum-mediated immunoresponses to spermatozoa. | sixty male lewis rats were immunized in groups of 10 by: (i) conventional vasectomy with ligation of the vas deferens; (ii) vasectomy without ligation of the vas deferens; (iii) sham vasectomy (control); (iv) subcutaneous injection of 10(9) allogeneic spermatozoa (from inbred bn rats) in complete freund's adjuvant (cfa); (v) subcutaneous injection of 10(9) syngeneic spermatoza in cfa; (vi) subcutaneous injection of 10(9) syngeneic spleen cells in cfa (control). one in vivo and one in vitro assay ... | 1975 | 1191388 |
| [humoral immunity in mice with transplantable leukemia during chemoimmunotherapy]. | the article treats of the effect of cyclophosphamide (cp), and complete freund's adjuvant (cfa) and their combination on the development of transplantable leukemia-hemocytoblastosis in mice and some host humoral immune reactions. the optimal combinations of the cp (on the 3rd day after the transplantation of leukemia) and of the cfa (on the 10th day) led to increase in the average survival up to 93,9 days (8.5 days in control). this combination also increased the survival of 42.9% of the animals ... | 1975 | 1241519 |
| the effect of beta aminoproprionitrile (bapn) on experimental amyloidosis. | experimental amyloidosis was induced in mice with repeated injections of complete freund's adjuvant (cfa) reinforced with bacterial vaccine. bapn administered in a mixture with cfa or on its own before the injection of cfa reduced the incidence of amyloidosis. the reduction in the incidence of amyloidosis following the administration of bapn may be due to its inhibitory effect on the oxidative deamination of amino acids, which presumably inhibit cross-linking of amyloid fibrils or interfere with ... | 1975 | 1212427 |
| selection and properties of escherichia coli mutants defective in the synthesis of cyclopropane fatty acids. | mutants of escherichia coli k-12 defective in the synthesis of cyclopropane fatty acids (cfa) have been selected and isolated by a l-[methyl-3h]methionine suicide procedure. two mutants were isolated. stationary-phase cultures of both mutants contain less than 0.7% of the cfa content found in the parental strain. the cfa deficiency is attributed to a deficiency of cfa synthetase activity. extracts of both mutants contain less than 10% of the cfa synthetase activity found in extracts of the paren ... | 1976 | 1107324 |
| role of cellular density, in vitro, in anti-tumor activity of cfa-treated and immunized cells. | incorporation of tritiated deoxythymidine (3hdt) into dna was used to measure growth, in vitro, of p815 tumor cells admixed with spleen and peritoneal effector cells. at a high tumor cell density ((1x10(5) cells per dish), using anti-theta and anti-macrophage sera, t-cells and macrophages from the peritoneum of immunized mice could be identified as cells possessing anti-tumor activity. a nonspecific inhibition by normal effector cells, which occurred at the high tumor cell density, did not occur ... | 1976 | 1008014 |
| the immune response of brown trout (salmo trutta l.) to injection with soluble antigens. | the immune response of brown trout (salmo trutta) to horse serum and keyhole limpet haemocyanin was studied. intraperitoneal and intramuscular injections were used, with and without adjuvant, in 209 fish. complement-fixing antibodies (cfa) and precipitins were produced to both antigens. cfa were detected after 8 days to haemocyanin and after 13 days to horse serum. maximum cfa titres to a single intraperitoneal injection of horse serum or haemocyanin were reached at 44 and 43-46 days respectivel ... | 1976 | 1022141 |
| in vitro studies on the enhancement of rauscher virus-induced erythroblastosis by complete freund's adjuvant in balb/c mice. | inoculation of complete freund's adjuvant (cfa) into balb/c mice either before or after infection with rauscher murine leukemia virus (mulv-r) led to an acceleration of the disease as determined by spleen weight. treatment with cfa also induced a higher number of spleen erythroblast foci and, in the bone marrow, erythropoietin-independent cells that produced erythroid colonies in vitro. cfa induced in the bone marrow not only an increase in myeloid progenitor cells that can produce colonies in a ... | 1976 | 1069856 |
| immunopathogenesis of autoimmune tubulointerstitial nephritis. ii. role of an immune response gene linked to the major histocompatibility complex. | immunization of strain xiii guinea pigs with rabbit renal tubular basement membranes (tbm) in cfa consistently results in severe autoimmune tubulointerstitial nephritis (tn). strain ii guinea pigs similarly immunized do not develop this disease. f1 guinea pigs were found to be intermediate between inbred strain ii and xiii animals with regard to extent of autoimmune tn and anti-tbm antibody production. the f1 x ii backcrosses segregated into two groups with regard to antibody production and dise ... | 1976 | 993586 |
| the effect of halofenate or halofenate free acid on human, rat and guinea pig platelet aggregation. | halofenate free acid (hfa), the major metabolite of the hypolipemic agent halofenate, blocked the secondary phase of human platelet aggregation induced by adp, epinephrine, or thrombin; higher concentrations of clofibrate free acid (cfa) were required to produce similar inhibitory effects on platelet aggregation. hfa and cfa inhibited collagen-induced aggregation of human, rat, or guinea pig platelets. halofenate orally administered to rats caused inhibition of collagen-induced aggregation when ... | 1976 | 989636 |
| induction of cellular and humoral immunological responsiveness to a soluble cercarial antigen preparation from schistosoma mansoni. | the development of immunological responsiveness to a soluble cercarial antigenic preparation (cap) from schistosoma mansoni was analyzed in inbred cba/j mice infected with cercariae, one or multiple times, or sensitized using cap. repeated exposure to 75 cercariae at three weekly intervals (3x-75) or subcutaneous administration of 20 mug of cap (cap/complete freund adjuvant [cfa]) stimulated the development of specific anti-cap lymph node cell blastogenesis. the degree of responsiveness was depe ... | 1976 | 971960 |
| the effect of levamisole on adjuvant arthritis in the rat. | levamisole (lev), an immunostimulatory agent, was studied for its effect on adjuvant disease of the rat. the drug was administered continuously according to three schedules: (a) seven days prior to immunization with complete freund's adjuvant (cfa); (b) simultaneously with cfa; or (c) 14 days after cfa. control animals received cfa alone, lev alone, or were sham treated with saline. three weekly evaluations were made, recording the general condition, weight curves, joint scores, and volume of hi ... | 1976 | 950633 |
| a method for producing anti-dna antibodies in ascitic fluid of mice. | immunological methods for the production of antibodies to dna in ascitic fluid of ddy, a/he, and c57b1/6 mice were investigated. relatively large volumes of ascitic fluid containing antibodies for dna in high concentration were obtainable after seven injections of denatured ssdna-mbsa complexes emulsified with cfa into peritoneal cavities of ddy mice. the quantity of anti-ascitic fluid from 4 to 5 ddy mice was equivalent to that of antiserum from one rabbit. ddy mice accumulation of ascites is m ... | 1976 | 932438 |
| analysis of the intradermal response against a soluble cercarial antigenic preparation from schistosoma mansoni. | intradermal testing was performed with a soluble cercarial antigenic preparation (cap) from schistosoma mansoni cercariae in cba/j mice multiply infected with s. mansoni or sensitized with cap. both an early (5-h) response and a late (24- to 48-h) reaction to cap, as measured by increase in dermal thickness, was elicited after injection of antigen into the ears of either multiply infected (3x-75) or cap-sensitized (cap/complete freund adjuvant [cfa]) mice. histopathological examination showed th ... | 1976 | 971961 |
| morphology of experimental, organ-specific insulitis of the mouse pancreas. | the morphologic and metabolic effect of a single intracutaneous injection of homologous endocrine pancreas in freund's complete adjuvant (cfa) was studied in 100 mice and compared with control groups which had been (1) immunized with murine insulin in cfa, (2) injected with cfa alone, or had (3) received no treatment. there were no differences between the control groups as regards the morphology of the pancreatic islets, and the glucose tolerance was normal. mice immunized with islet homogenate ... | 1976 | 785924 |
| antibody-mediated basophil accumulations in cutaneous hypersensitivity reactions of guinea pigs. | cutaneous basophil hypersensitivity (cbh) was studied in guinea pigs by using simplified histologic techniques. animals immunized with oxazolone or picryl conjugates of keyhole limpet hemocyanin (klh) emulsified with complete (cfa) or incomplete freund's adjunvant (ifa) were found to have hapten-specific cutaneous basophil reactions when skin tested at 1 week with oxazolone or picryl chloride contant painting or intradermal injection of oxazolone or picryl-conjugated human serum albumin, respect ... | 1976 | 932424 |
| immunopathogenesis of autoimmune tubulointerstitial nephritis. i. demonstration of differential susceptibility in strain ii and strain xiii guinea pigs. | autoimmune tubulointerstitial nephritis (tn) was induced in strain xiii and hartley but not strain ii guinea pigs after immunization with rabbit tubular basement membranes (tbm) in cfa. strain xiii guinea pigs developed extensive autoimmune tn associated with high anti-tbm (atbm) antibody titers and linear deposits of igg along renal cortical tbm after immunization with 10 mug to 10 mg of tbm. in addition, autoimmune tn was passively transferred to strain xiii animals by the i.p. injection of at ... | 1976 | 765397 |
| effects of androstenes, 5alpha-androstanes, 5beta-androstanes, oestrenes and oestratrienes on serum gonadotrophin levels and ventral prostate weights in gonadectomized, adult male rats. | the morphologic and metabolic effect of a single intracutaneous injection of homologous endocrine pancreas in freund's complete adjuvant (cfa) was studied in 100 mice and compared with control groups which had been (1) immunized with murine insulin in cfa, (2) injected with cfa alone, or had (3) received no treatment. there were no differences between the control groups as regards the morphology of the pancreatic islets, and the glucose tolerance was normal. mice immunized with islet homogenate ... | 1976 | 785923 |
| studies on mechanisms of immobilization of mononuclear cells in the delayed hypersensitivity (dh) reaction. | migration inhibitory (mi) activity in exudates, and 'migration' and 'inhibition of migration' of exudate cells was investigated in the delayed hypersensitivity (dh) reaction induced by intrapleural injection of ppd into complete freund's adjuvant(cfa)-sensitized guinea-pigs. during the initial reaction (6-hour), two types of antigen-dependent mi activity were detected in serum and cell free exudate. one was a high molecular weight material associated with immunoglobulin, and the other was a low ... | 1976 | 782206 |
| cutaneous basophil hypersensitivity uncovered in the cell transfer of classical tuberculin hypersensitivity. | cellular transfer of cutaneous basophil hypersensitivity (cbh) was studied. guinea pigs immunized for cbh with incomplete freund's adjuvant (ifa) provided cells which could transfer delayed and basophil-rich reactions in skin tests of recipients. guinea pigs immunized with complete classical tuberculin-type delayed hypersensitivity reactions (dh), which are characteristically devoid of basophils. however, recipients of cells from donors with dh, surprisingly, were found to have delayed skin reac ... | 1976 | 1085308 |
| experimental uveitis induced by prostatic antigens. | experimental uveitis was induced in male rats following their repeated systemic and intravitreous immunization with prostatic antigen and cfa. the ensuing uveitis was proved histologically. heteroimmunized rabbits and female animals showed less response and the level of antibodies detected was lower. anti-prostatic antibodies were determined by the trc flocculation and pca techniques. ouchterlony's method gave positive results not only for prostatic but also for other ocular antigens when tested ... | 1976 | 1250224 |
| in vitro hemolysis of autologous erythrocytes caused by immune murine spleen cells and spherules of the fungus coccidioides immitis. | this communication describes an in vitro system wherein mouse erythrocytes are lysed in the presence of spherules of the fungus coccidioides immitis and spleen cells from syngeneic mice immunized with a variety of antigens. the antigens include: tobacco mosaic virus in complete freund's adjuvant (cfa), cfa alone, separate components of cfa, sheep erythrocytes, and allogeneic tumor. spleen cells from mice sublethally infected with c. immitis are also capable of participating in this response. the ... | 1976 | 1254972 |
| the effect of complete freund--adjuvant on chronic proliferating inflammation in an experimental granuloma model. | histometric, biochemical, radiochemical and autoradiographic studies were undertaken to investigate the influence of complete freund-adjuvant (cfa) on a defined chronic proliferating inflammation of a granuloma model in two different experimental situations. where as the percentage fraction of the fibroblasts, 3h-thymidine marking index of the fibroblasts and the impulse rate of the fibroblasts and endothelial cells do not differ from the values found for the control animals, both the protein an ... | 1976 | 1266421 |
| t cell requirement for experimental allergic encephalomyelitis induction in the rat. | the question of whether a cell-mediated or a humoral mechanism initiates eae in rats sensitized with bp-cfa was investigated. the requirement of t cells for eae induction was manifested when tx, irradiated rats were reconstituted with normal lymphoid cells treated with ats and then injected with bp-cfa. neither eae nor antibody was produced, indicating the t cell dependency of bp specific antibody production. more precise information regarding the role of the t cell in the production of eae was ... | 1976 | 1084904 |
| lung water and capillary permeability in pulmonary sarcoid-like granulomatosis. | the subject of our research was the use of multiple indicators to study the lungs of rats during a pathological process induced by complete freund's adjuvant (cfa). the parameters studied were extravascular water (idqwl), the capillary permeability of [14c]sucrose, and the unrecovered fraction of [3h5glucose. we ascertained that idqwl gives a valuable estimate of total pulmonary water (r=0.91, n=30) and allows a satisfactory quantification of granulomatosis, as is shown by the comparison between ... | 1976 | 1067016 |
| immunological characteristics of the armadillo, dasypus sabanicola. | the immunological responses of the armadillo are of interest because of its susceptibility to generalized lepromatoid infection with mycobacterium leprae. in this study, specimens of dasypus sabanicola were found to have a typical mammalian distribution of lymphoid cells in thymus, spleen, lymph nodes and blood. their complement was active in bactercidal, protozoan immobilization and haemolytic systems. blood lymphocytes responded to phytohaemagglutinin and to pokeweed mitogen. sensitization wit ... | 1976 | 825337 |
| cell-mediated hypersensitivity in guinea-pigs infected with toxoplasms gondii. | this investigation demonstrated delayed hypersensitivity by macrophage migration inhibition (mmi) and skin-testing (st) at 4, 8, 12, and 17 weeks and by lymphocyte transformation (lt) at 4, 12, and 17 weeks after infection of guinea-pigs (gp) with toxoplasma gondii (c-37 strain). mmi and lt were both most pronounced at 4 and 17 weeks post-infection. gp immunized with toxoplasmin in complete freund's adjuvant (cfa) demonstrated positive blast transformation and st, but gp immunized with phosphate ... | 1976 | 134544 |
| dissociation of mif activity and in vitro lymphocyte transformation in the development of experimental allergic thyroiditis in guinea pigs. | macrophage migration inhibitory factor (mif) activity and in vitro lymphocyte transformation to homologous thyroid antigen were compared in cultures of lymph node cells obtained from 123 guinea pigs at weekly intervals after a single injection of homologous thyroid extracts in complete freund's adjuvant (cfa). mif activity was not detectable at the early stage of immunization but became manifest 2 and 3 weeks after sensitization and a high level was maintained during 4-7 weeks. on the other hand ... | 1976 | 790649 |
| distribution of plaque-forming cells in the mouse for a protein antigen. evidence for highly active parathymic lymph nodes following intraperitoneal injection of hen lysozyme. | the distribution of plaque-forming cells (pfc) throughout the lymphoid system of cba mice was followed with time after a primary intraperitoneal injection of hen egg white lysozyme emulsified in freund's complete adjuvant (hel-cfa) and after a secondary soluble injection. throughout the primary response (predominantly igg) and during the first week of the secondary response (exclusively igg), the highest density of pfc was found in the draining parathymic lymph nodes, followed by the local splee ... | 1976 | 800396 |
| hypergammaglobulinemia in chickens congenitally infected with an avian leukosis virus. | significantly elevated (2- to 5-fold higher than controls) serum levels of igg were found in chickens congenitally infected with f42 strain of avian leukosis (alv-f42) a subgroup a avian leukosis virus (alv). a further increase in igg levels in congenitally infected birds was found to be induced by injection of influenza virus in complete freund's adjuvant(cfa). serum immunoglobulin m (igm) levels were not significantly elevated in alv congenitally infected chickens except in those animals that ... | 1976 | 185293 |
| hormone independent in vitro erythroid colony formation by mouse bone marrow cells. | b.m. cells of rlv-infected balb/c mice can proliferate in methylcellulose in the absence of e.p., while normal b.m. cells cannot (12). not only the more primitive bfu-e shows hormone-independency (18). this phenomenon is in favour of the view that the rauscher virus induced erythroblastosis is a true neoplasia although transplantation experiments failed so far. the experiments in which transformation in vitro of b.m. cells by rlv is established (19) show that the cfu-e can serve as a target for ... | 1976 | 188723 |
| protection against experimental allergic encephalomyelitis with peptides derived from myelin basic protein: presence of intact encephalitogenic site is essential. | experimental allergic encephalomyelitis (eae) is a cell-mediated autoimmune response directed toward a component of central nervous system (cns) tissue, myelin basic protein (bp). injection of animals with either whole cns tissue or purified bp in complete freund's adjuvant (cfa) induces severe and usually fatal disease. preimmunization of animals with bp in incomplete freund's adjuvant (ifa) prevents eae. we have examined the relative abilities of whole guinea pig bp and its fragments to protec ... | 1976 | 58930 |
| [studies on the significance of antigen in the 6-mercapopurine-influenced humoral antibody response in guinea pigs]. | the mechanism of suppression of humoral immune response to dinitrophenylated bovine gamma globulin (dnp23-bgg), human serum albumin (hsa), and trinitrophenylated sheep red blood cells (tnp-srbc) by 6-mercaptopurine (6-mp) was studied in guinea pigs. following the intradermal application of the antigens emulsified in complete (cfa) or incomplete freund's adjuvant (ifa) each test animal was given 6-mp, 10 mg/kg/day for 7 days. this treatment resulted in a significant suppression of the anti bgg an ... | 1976 | 69376 |
| ontogenetic development of conditioned food aversion in chickens. | development of conditioned food aversion (cfa) was studied in 25-, 35-, and 45-day leghorn chicks. food-deprived birds had 10-min access to normal food on days 1 and 2, and to green coloured food cs on day 3. injection of licl (0.15 m, 3-4% body weight) administered 10 min after cs on day 3 served as the us. control groups were injected with the same volume of saline. retention was tested on day 4 in three 3-min presentations of normal food alternating with two presentations of green food. numbe ... | 1976 | 24923745 |
| adjuvant regulation of t cell function. | the effect of complete freund's adjuvant (cfa) on distinct t cell functions was investigated. adjuvant was found to suppress the generation of cytolytic t cells in vivo when mixed with allogeneic p815 cells before immunization of c57bl/6 mice. inoculation of the mice with either adjuvant or adjuvant emulsified with allogeneic cells resulted in whole splenic populations or immunoabsorbent-purified t cells that did not generate cytolytic activity in vitro against allogeneic cells. mixing t cells f ... | 1976 | 1082892 |
| [cryptogenetic fibrose alveolitis (cfa). apropos of 13 cases]. | 1976 | 1257526 | |
| morphological, biochemical, and serological studies on a viral agent (cfa) which replicates in and causes fusion of aedes albopictus (singh) cells. | 1976 | 982814 | |
| studies of sperm antigenicity. 6. in vivo and in vitro cellular reactivity in guinea pigs sensitized with fractions of guinea pig spermatozoa. | normal guinea pig spermatozoa cells were homogenized by a french pressure cell. three soluble and three insoluble fractions were obtained by ultrascentrifugation and (emulsified in cfa) were used for guinea pig sensitization. the following were observed: 1) all fractions were immunogenic except one; 2) in vivo and in vitro delayed hypersensitivity was elicited in animals immunized with these fractions; 3) two distinctive histopathologic lesions were observed in the testes of sensitized animals: ... | 1977 | 69611 |
| immune ascites in the guinea pig: specificity of cells and antibody in an induced peritoneal exudate. | strain 2 guinea pigs, immunized with lys10-lys(dnp) in cfa and repeatedly injected intraperitoneally with adjuvant, developed ascites. the fluid was harvested over 8 months in total amounts up to 2 liters per animal and contained substantial amounts of cells and antibody which reacted with the immunizing antigen and related peptides. the antibody was of the igg and iga classes and showed restricted heterogeneity. among synthetic dnp-oligopeptides, both the cells, studied by antigen-stimulated th ... | 1977 | 70481 |
| evidence for multiple cell surface chicken fetal-leukemic antigens (cfa) in the developing chick and other avian species. | chicken fetal antigen (cfa), a membrane antigen present on fetal chicken red blood cells is lost with chicken development, and reappears on the red blood cells of leukemic chickens. seven avian species were found to possess cfa. a species hierarchy comparing the quantitative expression of cfa has been established. the levels of cfa expression with development are compared in the chicken and japanese quail. specific adsorptions of r-anti-cfa with avian red blood cells revealed the existence of mu ... | 1977 | 72784 |
| studies on the encephalitogenic effects of purified preparations of human and bovine oligodendrocytes. | bulk-isolated human and bovine oligodendroglia, practically free from myelin, have been used in attempts to elicit an autoimmune response which has been compared with acute experimental allergic encephalomyelitis (eae). for these experiments, a total of 20 hartley guinea pigs, 33 lewis rats and 16 rabbits have been studied. animals were inoculated with a range of doses of purified preparations of both human and bovine oligodendroglial cells in complete freund's adjuvant (cfa) and compared with o ... | 1977 | 64280 |
| experimental cell-mediated interstitial nephritis induced with exogenous antigens. | the capacity of intrarenal injections of bovine gamma-globulin (bgg) to elicit hypersensitivity reactions was studied in guinea pigs immunized with dnp bgg or bgg immune complexes in cfa or in rats immunized with bgg in cfa. in guinea pigs it was found that heat-aggregated bgg elicited inflammatory reactions in the renal cortex, whereas soluble bgg did not. in rats only aggregated bgg was used, and this was found to be effective. the reactions were characterized by a predominantly mononuclear ce ... | 1977 | 65431 |
| [studies on antigenspecific immunosuppression: influence of 6-mercaptopurine-bgg-conjugates on bgg binding cells (author's transl)]. | the rosette forming cell (rfc) response of different lymphoid organs (spleen, lymph node, peripheral blood) of guinea pigs was examined by immunocytoadherence. the method has been adapted to work with the soluble antigen bgg. the number of antigen binding cells following immunization with bgg in cfa reached the highest level on day 6 in ln. popliteus and on the 10th day in spleen and peripheral blood. it is shown that the highest level of antigen binding cells appears generally before the maximu ... | 1977 | 76446 |
| correlation of exposure to various respiratory pathogens with farmer's lung disease. | complement-fixing antibodies (cfa) to a panel of microorganisms commonly associated with respiratory disease were measured in a number of agricultural populations. the panel included mycoplasma pneumoniae, influenza viruses a and b, parainfluenza virus types 1, 2, and 3, adenovirus, and respiratory syncytial virus. the agricultural populations were grouped according to a clinical history of farmer's lung disease (fld) and the presence of antibodies to the thermophilic actinomycetes (ta). farmers ... | 1977 | 197136 |
| relation of a cross-reactive idiotype to genetic control of the immune response. | antibodies elicited in strain a mice by immunization with keyhole limpet hemocyanin-p-azophenylarsonate (klh-ar) produce anti-ar antibodies, some of which share a cross-reactive idiotype (cri); in general, 20 to 70% of the antihapten antibody population carries the idiotype. large amounts of antibody can be produced by the induction of ascitic fluids, using a 9:1 ratio of complete freund's adjuvant (cfa) to antigen. antibodies with the cri can be isolated by isoelectric focusing from selected mi ... | 1977 | 300060 |
| pathogenesis of experimental allergic orchitis. iii. t lymphocyte requirement in local adoptive transfer by peritoneal exudate cells. | in experimental allergic orchitis (eao), a lesion characterized by mononuclear invasion of seminiferous tubules can be adoptively transferred within 1 to 4 days by testicular injection of peritoneal exudate cells (pec) from syngeneic strain 13 guinea pigs (gp) immunized with homologous testicular antigens in complete freund's adjuvant (cfa). this study examined the role of t lymphocytes, macrophages, and polymorphonuclear neutrophils (pmn) in the adoptive transfer. guinea pig pec contained 7% t ... | 1977 | 300759 |
| treatment of autologous immune complex glomerulonephritis with vasoactive amine antagonists. | aic nephritis produced in rats by a single injection of fxia in cfa was treated by agents with known antihistamine and antiserotonin activity. the effect of this drug regimen in altering the deposition of ag-ab complexes in the glomerulus was assessed by light, immunofluorescent, and electron microscopy. the vasoactive amine antagonists chlortrimeton, vistaril, azatadine, and sansert, when given singly or in combinations which completely abrogated histamine and serotonin-induced skin wheal forma ... | 1977 | 143501 |
| cuta neous basophil responses in neonatal guinea pigs: active immunization, hapten specific transfer with small amounts of serum, and preferential elicitation with phytohemagglutinin skin testing. | previous studies have demonstrated a failure to elicit delayed hypersensitivity skin responses in neonatal guinea pigs despite apparent immunologic competence of their lymphocytes and monocytes. the ability of neonatal guinea pigs to manifest cutaneous basophil hypersensitivity (cbh) responses was examined. neonates were competent to express macroscopic and microscopic aspects of these delayed reactions. strong cutaneous basophil responses were elicited in newborns (0 to 2 days old) in a hapten- ... | 1977 | 845431 |
| relation between delayed skin reactivity and macrophage migration inhibition or lymphocyte transformation in tuberculin-type hypersensitivity and jones-mote hypersensitivity. | precise time-course studies on delayed skin reaction, lymphocyte transformation and macrophage migration inhibition were carried out from day 3 to 270 and from day 3 to 120, respectively, in guinea pigs immunized with bovine gamma-globulin (bgg) in complete freund's adjuvant (cfa) and those immunized with bgg in incomplete freund's adjuvant (ifa). a) delayed skin reactions could be elicited for a long period of time after immunization with bgg in cfa in the presence of prominent antibody product ... | 1977 | 859455 |
| morphology of delayed-type hypersensitivity reactions in the guinea pig cornea. | guinea pigs sensitized with several soluble protein antigens in incomplete freund's adjuvant (ifa) and tested at 5 to 7 days developed basophil-rich corneal reactions characterized by minimal edema and fibrin deposition. these lesions resembled the skin reactions of cutaneous basophil hypersensitivity (cbh) in their immunization requirements, time course, and character, but differed in that basophils were relatively less frequent and eosinophils and neutrophils more numerous in ocular than in cu ... | 1977 | 870560 |
| induction of autoimmunity in good and poor responder mice with mouse thyroglobulin and lipopolysaccharide. | the administration of soluble mouse thyroglobulin (mtg) in conjunction with bacterial lipopolysaccharide (lps) led to the termination of natural tolerance to mtg in mice. the extent of autoimmunity correlated with responsiveness to mtg, previously shown by the injection of mtg in complete freund's adjuvant (cfa) to be dependent upon the h-2 haplotype. in good responder b10.br (h-2k) mice given mtg either with lps or in cfa, high antibody levels to mtg and extensive mononuclear cell infiltration ... | 1977 | 323406 |
| functional, structural, and antigenic similarities of guinea pig anti-phosphorylcholine antibodies. | the humoral immune response to pc was measured in guinea pigs. pc-vaccine stimulated igm and igg2, but little igg1, anti-pc -antibodies. no memory was induced and immunization in cfa produced tolerance. pc-klh, on the other hand, stimulated igm, igg2, and igg1 anti-pc antibodies with carrier-specific memory. hapten inhibition of plaque formation showed uniform binding patterns with minor, but significant, differences between antipc-vaccine and anti-pc-klh antibodies. the antibodies were characte ... | 1977 | 325132 |
| streptococcal-induced cell-mediated-immune destruction of cardiac myofibers in vitro. | we have demonstrated that t lymphocytes from the spleens of adult guinea pigs sensitized to group a streptococcal antigens are cytotoxic for cultured fetal guinea pig heart cells. lymphocyte cytotoxicity, measured by 51cr release from target cells, was stimulated by sensitization in vivo with group a whole cells, cell walls, and purified protoplast membranes emulsified with complete freund's adjuvant (cfa). sensitization with group c streptococcal antigens in cfa or cfa alone produced lymphocyte ... | 1977 | 327015 |
| adjuvant protection against bacterial infection in granulocytopenic mice. | the hypothesis that the induction of nonspecific resistance to infection by immunostimulation prior to drug-induced granulocytopenia would afford increased protection to subsequent bacterial challenge was tested in a murine model of infection with pseudomonas aeruginosa or staphylococcus aureus in mice rendered granulocytopenic with cyclophosphamide. prior intraperitoneal immunostimulation of mice with complete freund's adjuvant (cfa) or mycobacterium bovis (bacille calmette-guèrin; bcg) increas ... | 1977 | 328788 |
| hemagglutination of human group a erythrocytes by enterotoxigenic escherichia coli isolated from adults with diarrhea: correlation with colonization factor. | enterotoxigenic escherichia coli (etec) of several different serotypes isolated from adults with diarrhea and known to possess the colonization factor antigen (cfa) were found to cause mannose-resistant hemagglutination (ha) of human group a erythrocytes. cfa-negative e. coli isolated during the same study did not possess the mannose-resistant hemagglutinin, although some non-etec, cfa-negative isolates did exhibit mannose-sensitive ha activity. the mannoseresistant hemagglutinin of etec was fou ... | 1977 | 336541 |
| effects of 2.6-4.0 ghz microwave radiation on e-coli b. | the effects of 2.6-4.0 ghz microwave radiation on living e. coli b bacteria were studied using measurement of the colony forming ability (cfa) of the cells and alterations in the molecular structure determined by comparing the infrared spectrum of irradiated and unirradiated cell cultures. at absorbed power levels of 20 mw in 1 ml of cellular suspensions (i.e., a specific absorption rate of 20 w/kg) for 10-12 hour exposures, no effects were observed on either the molecular structure or the cfa f ... | 1977 | 336894 |
| adjuvant properties of micropolyspora faeni. | the adjuvant properties of micropolyspora faeni, an important source of antigenic material in the production of farmer's lung, were evaluated by comparing antibody- and cell-mediated immune responses of rabbits to bovine serum albumin (bsa) incorporated in complete freund's adjuvant (cfa), incomplete freund's adjuvant (ifa) and incomplete freund's adjuvant with 5-10 mg/ml homogenized m. faeni (mfa). rabbits immunized with bsa in cfa or mfa developed significantly increased antigen-induced macrop ... | 1977 | 338504 |
| suppression of acute and chronic experimental allergic encephalomyelitis in strain 13 guinea pigs. a clinical and pathological study. | adult inbred strain 13 guinea pigs develop an acute, fatal form of experimental allergic encephalomyelitis (eae) about 2 weeks after a single injection of isologous spinal cord in complete freund's adjuvant (cfa), but similarly injected juveniles develop a delayed, rarely fatal chronic form. thirty-seven sensitised adult strain 13 animals were separated into 2 groups. one group was permitted to develop acute eae. the other group was injected intramuscularly with 1 mg of guinea pig or bovine myel ... | 1977 | 845617 |
| c. parvum skin testing antigen: study on guinea pig model. | a skin-testing antigen produced from c. parvum has been developed for exploring cell-mediated immunity and specially c. parvum specific cell-mediated immunity by delayed cutaneous hypersensitivity (dch) reaction. the dch antigen and the techniques of intradermal injection and multiple puncture are described. dch reactions are carried out in c. parvum specifically sensitized guinea pigs: sensitization procedure and adjuvant (ifa and cfa) effects are reported. measures of dch reactions for differe ... | 1977 | 608551 |
| regulation of antibody response in different immunoglobulin classes. ii. induction of in vitro ige antibody response in murine spleen cells and demonstration of a possible involvement of distinct t-helper cells in ige and igg antibody responses. | in vitro induction of anti-dnp ige as well as igg1, igg2a antibody responses was shown in murine spleen cell culture. spleen cells primed three times with 1 mug of dnp-oa or dnp-asc produced significant amounts of anti-dnp ige as well as igg antibodies by the in vitro stimulation with dnp-oa or dnp-asc, respectively. collaboration between dnp-primed b cells and carrier-primed t cells was required for the induction of both ige and igg antibodies with dnp-coupled t-dependent antigen. carrier-speci ... | 1977 | 300389 |
| histochemical studies on the ebv-determined nuclear antigen (ebna). | the ebv-determined nuclear antigen (ebna) was studied with regard to several histochemical properties. proteolytic enzymes destroyed ebna staining. rnase dnase and hyaluronidase had no effect on the number of ebna positive cells. intensive treatment with dnase weakened the chromosomal fluorescence of ebna, whereas the staining of interphase nuclei was relatively enzyme resistant. when ebv-associated soluble complement-fixing antigen of raji cells (cfa-r) was added to methanolacetic acid-fixed ch ... | 1977 | 200290 |
| lymphocyte specificity to protein antigens. i. characterization of the antigen-induced in vitro t cell-dependent proliferative response with lymph node cells from primed mice. | an in vitro assay which measures antigen-induced proliferation of primed murine lymph node cells is described. the response is mediated by t eclls since it can be obtained by using nylon wool-passed lymphocytes (less than 1% ig+ cells) and it can be abolished by treatment with anti-thy 1.2 and c. furthermore, ln cells from nu/nu mice injected with antigen do not demonstrate antigen-induced proliferation in contrast to the response observed in euthymic littermate controls. other relevant paramete ... | 1977 | 70468 |
| the possible role of the carcinoembryonic antigen (cea) and other carcinofetal antigens in maligant and benign diseases of the gastrointestinal tract. | in the present review we have discussed antigens, principally the cea, which have their well defined place in the clinical management of the (malignant) diseases of the gastrointestinal tract. though the immunological diagnosis of neoplasia is one of the research areas where the most effort and hopes are invested, it is also there, that the carcinofoetal antigens have the least usefullness at the moment. however, studies like those undertaken by edgington and plow are probably pointing out if no ... | 1977 | 74140 |