Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| oral administration of tea saponins to relive oxidative stress and immune suppression in chickens. | the present study was designed to evaluate the effects of tea saponins on oxidative stress induced by cyclophosphamide in chickens. one hundred twenty chickens were randomly divided into 5 groups. groups 3 to 4 received intramuscular injection of cyclophosphamide to induce oxidative stress and immunosuppression. after that, groups 2 and 4 were orally administered tea saponins in drinking water for 7 d. then, groups 1 to 4 were immunized with a live, bivalent vaccine of newcastle disease virus an ... | 2017 | 28633386 |
| analysis of antigenicity and pathogenicity reveals major differences among qx-like infectious bronchitis viruses and other serotypes. | avian coronavirus infectious bronchitis virus (ibv) causes considerable damage to the poultry industry worldwide and the proportion of qx-like genotype isolates have increased over time. here, to better understand the antigenicity and pathogenicity of this genotype, we conducted sequence analyses, cross neutralization tests, and also examined the pathogenicity of two strains, sd and sz. sequence analyses revealed that sd and sz isolates belong to the qx-like ibv genotype and share high homology ... | 2017 | 28619139 |
| genetic, antigenic, and pathogenic characteristics of avian infectious bronchitis viruses genotypically related to 793/b in china. | in this study, 20 infectious bronchitis virus (ibv) strains, which were genotypically related to 793/b, as assessed by an s1 gene comparison and a complete genomic sequence analysis, were isolated and identified from 2009 to 2014 in china. phylogenetic analysis, network tree, similarity plot analysis, recombination detection program 4(rdp4) and sequence comparison revealed that 12 of the 20 isolates were likely the reisolated vaccine virus. one isolate, ck/ch/lsd/110857, was shown to have origin ... | 2017 | 28619134 |
| co-infection of h9n2 subtype avian influenza virus and infectious bronchitis virus decreases sp-a expression level in chickens. | chicken surfactant protein a (csp-a) is a collectin believed to play an important role in antiviral immunity. however, csp-a expression in the respiratory tract of chickens after viral co-infection remains unclear. the aim of this study was the detection and characterization of csp-a in co-infected chickens. for this purpose, four-week-old specific pathogen-free (spf) chickens were divided into five groups and inoculated intranasally with h9n2 subtype avian influenza virus (aiv), infectious bron ... | 2017 | 28619132 |
| a reverse genetics system for avian coronavirus infectious bronchitis virus based on targeted rna recombination. | avian coronavirus infectious bronchitis virus (ibv) is a respiratory pathogen of chickens that causes severe economic losses in the poultry industry worldwide. major advances in the study of the molecular biology of ibv have resulted from the development of reverse genetics systems for the highly attenuated, cell culture-adapted, ibv strain beaudette. however, most ibv strains, amongst them virulent field isolates, can only be propagated in embryonated chicken eggs, and not in continuous cell li ... | 2017 | 28606144 |
| antigen-sparing and enhanced efficacy of multivalent vaccines adjuvanted with immunopotentiators in chickens. | we previously described that immunopotentiators, cvcva5, increased the efficacy of h5 and h9 subtype avian influenza vaccines in chickens, ducks, and geese. in this study, we further investigated the effects of the cvcva5 for improving the efficacy of other univalent or multivalent inactivated vaccines. the immune response administrated with half-dose of monovalent vaccine plus cvcva5 were higher than those of one dose of monovalent vaccine without immunopotentiators as measured by levels of ant ... | 2017 | 28603519 |
| infectious bronchitis virus in romanian broiler farms. | 2017 | 28600421 | |
| combination of h120 and 1/96 avian infectious bronchitis virus vaccine strains protect chickens against challenge with is/1494/06 (variant 2)-like infectious bronchitis virus. | avian infectious bronchitis (ib) is a worldwide chicken disease, caused by avian infectious bronchitis virus (ibv) which infects all commercial poultry lines. the present study was done to evaluate protection caused by two different serotype vaccines (massachusetts and 793/b) in order to evaluate protection against challenge with is/1494/06-like virus (variant 2-like virus), which is prevalent in the middle east. spf chickens were divided into four groups (n = 20). first and second group as nega ... | 2017 | 28523921 |
| reverse genetics system for the avian coronavirus infectious bronchitis virus. | we have developed a reverse genetics system for the avian coronavirus infectious bronchitis virus (ibv) in which a full-length cdna corresponding to the ibv genome is inserted into the vaccinia virus genome under the control of a t7 promoter sequence. vaccinia virus as a vector for the full-length ibv cdna has the advantage that modifications can be introduced into the ibv cdna using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. ... | 2017 | 28508215 |
| complete genome sequences of two avian infectious bronchitis viruses isolated in egypt: evidence for genetic drift and genetic recombination in the circulating viruses. | avian infectious bronchitis virus (ibv) is highly prevalent in chicken populations and is responsible for severe economic losses to poultry industry worldwide. in this study, we report the complete genome sequences of two ibv field strains, cu/1/2014 and cu/4/2014, isolated from vaccinated chickens in egypt in 2014. the genome lengths of the strains cu/1/2014 and cu/4/2014 were 27,615 and 27,637 nucleotides, respectively. both strains have a common genome organization in the order of 5'-utr-1a-1 ... | 2017 | 28495648 |
| specific detection of gii-1 lineage of infectious bronchitis virus. | infectious bronchitis virus (ibv) is a worldwide prevalent rna virus that causes highly contagious and economically devastating disease in chicken. the virus exists in many different genetic forms which made the disease control very difficult. the present study describes the development and validation of taqman probe-based real-time reverse transcription-polymerase chain reaction (real-time rt-pcr) targeting the s1 coding region of s gene characteristic for the gii-1 lineage (formerly the d1466- ... | 2017 | 28493279 |
| pathogenicity study of iranian genotype of avian infectious bronchitis virus (ir-1). | avian infectious bronchitis (ib) is a major cause of economic losses in poultry industry. the ib virus primarily affects respiratory tract, but various strains differ in their tropism for other target organs such as kidney and alimentary tract. the objective of this study was to estimate the pathogenicity of iranian ibv variant (ir-1), which is limited exclusively to iran. specific pathogen free chicks were inoculated intranasally. sera, fecal swabs and different tissue samples were collected on ... | 2017 | 28473895 |
| different evolutionary trajectories of vaccine-controlled and non-controlled avian infectious bronchitis viruses in commercial poultry. | to determine the genetic and epidemiological relationship of infectious bronchitis virus (ibv) isolates from commercial poultry to attenuated live ibv vaccines we conducted a phylogenetic network analysis on the full-length s1 sequence for arkansas (ark), massachusetts (mass) and delmarva/1639 (dmv/1639) type viruses isolated in 2015 from clinical cases by 3 different diagnostic laboratories. phylogenetic network analysis of ark isolates showed two predominant groups linked by 2 mutations, consi ... | 2017 | 28472110 |
| development of a taqman-based real-time pcr assay for rapid and specific detection of fowl aviadenovirus serotype 4. | twelve serotypes of fowl aviadenovirus, namely, fadv-(1-8a and 8b-11), have been identified, among which fadv-4 is the aetiologic agent of hepatitis hydropericardium syndrome (hhs) in chickens. outbreaks of hhs have been documented in many countries, causing significant economic losses. real-time pcr methods described so far in the literature cross-detect different serotypes of fadvs. in this study, we aimed to develop a taqman-based real-time pcr assay for the specific detection of fadv-4. a pa ... | 2017 | 28437156 |
| phylogenetic analysis of avian infectious bronchitis virus isolates from morocco: a retrospective study (1983 to 2014). | 2017 | 28421419 | |
| the effect of diatomaceous earth in live, attenuated infectious bronchitis vaccine, immune responses, and protection against challenge. | live virus vaccines are commonly used in poultry production, particularly in broilers. massive application and generation of a protective local mucosal and humoral immunity with no adverse effects is the main goal for this strategy. live virus vaccines can be improved by adding adjuvants to boost mucosal innate and adaptive responses. in a previous study we showed that diatomaceous earth (de) can be used as adjuvant in inactivated vaccines. the aim of this study was to test de as adjuvant in an ... | 2017 | 28419351 |
| assessment of molecular and genetic evolution, antigenicity and virulence properties during the persistence of the infectious bronchitis virus in broiler breeders. | the infectious bronchitis virus (ibv) causes a highly contagious disease [infectious bronchitis (ib)] that results in substantial economic losses to the poultry industry worldwide. we conducted a molecular and phylogenetic analysis of the s1 gene of brazilian (br) ibv isolates from a routinely vaccinated commercial flock of broiler breeders, obtained from clinical ib episodes that occurred in 24-, 46- and 62-week-old chickens. we also characterized the antigenicity, pathogenesis, tissue tropism ... | 2017 | 28895517 |
| avian infectious bronchitis virus disrupts the melanoma differentiation associated gene 5 (mda5) signaling pathway by cleavage of the adaptor protein mavs. | melanoma differentiation associated gene 5 (mda5) and retinoic acid-inducible gene-i (rig-i) selectively sense cytoplasmic viral rna to induce an antiviral immune response. infectious bronchitis virus (ibv) is one of the most important infectious agents in chickens, and in chicken cells, it can be recognized by mda5 to activate interferon production. rig-i is considered to be absent in chickens. however, the absence of rig-i in chickens raises the question of whether this protein influences the ... | 2017 | 29132350 |
| [establishment of a high sensitive indirect elisa for detecting specific antibodies against h9 subtype avian influenza virus]. | h9 subtype avian influenza virus causes worldwide epidemic, resulting in enormous economic losses of poultry production. in the present study, an indirect elisa method was established for more accurate and specific detection. the recombinant protein of the globular head domain of ha of h9 subtype avian influenza virus was used as antigen. specific blocking buffers and dilution buffers were determined to increase the sensitivity and specificity. the sensitivity of elisa was higher than that of he ... | 2017 | 28853253 |
| astragalus polysaccharides enhance the immune response to avian infectious bronchitis virus vaccination in chickens. | astragalus polysaccharides (aps) are biological macromolecules extracted from astragalus species that have strong immunoregulatory properties. in this study, aps were employed as an adjuvant for an avian infectious bronchitis virus (ibv) vaccine, and its effects on the cellular immune and humoral immune responses to vaccination in chicken were investigated. one hundred and fifty chicken were randomly divided into five groups (n = 30, each group). the chickens in all groups, except for the unvacc ... | 2017 | 28826771 |
| genetic characterisation and analysis of infectious bronchitis virus isolated from brazilian flocks between 2010 and 2015. | infectious bronchitis virus (ibv) variants in brazil were isolated during 2010-2015 for epidemiological and molecular analysis to characterise the different variants and perform a bioinformatic analysis to compare with sequences of variants collected over the previous 40 years. of the 453 samples examined, 61.4% were positive for ibv and 75.9% of these were considered to have the br-i genotype and were detected in birds of all ages distributed in all five brazilian regions. the ratio of non-syno ... | 2017 | 28805451 |
| genome characterization, antigenicity and pathogenicity of a novel infectious bronchitis virus type isolated from south china. | in 2014, three infectious bronchitis virus (ibv) strains, designated as γcov/ck/china/i0111/14, γcov/ck/china/i0114/14 and γcov/ck/china/i0118/14, were isolated and identified from chickens suspected to be infected with ibv in guangxi province, china. based upon data arising from s1 sequence and phylogenetic analyses, the three ibv isolates were genetically different from other known ibv types, which represented a novel genotype (gi-29). virus cross-neutralization tests, using γcov/ck/china/i011 ... | 2017 | 28800976 |
| genotyping of avian infectious bronchitis viruses in iran (2015-2017) reveals domination of is-1494 like virus. | avian infectious bronchitis virus (ibv) is causing major economic losses to the poultry industry. the analysis of the s1 gene has been used to determine ibv genotype. the aim of this study was genotyping of ibvs circulating among the iranian broiler flocks in the period between 2015 to 2017. trachea samples from 278 broiler flocks were collected from broiler farms in eight provinces of iran. after real-time rt-pcr, ibv-positive samples were further characterized based on s1 gene. the results of ... | 2017 | 28789874 |
| microrna transcriptome analysis in chicken kidneys in response to differing virulent infectious bronchitis virus infections. | infectious bronchitis virus (ibv) can cause a highly contagious and acute respiratory disease in poultry. micrornas (mirnas) have emerged as a class of crucial regulators for gene expression and are involved in the regulation of virus defence and immunological processes. to understand mirna regulation in chickens in response to ibv infection, high-throughput sequencing was performed to compare the small rna libraries from the kidneys of chicken infected with sck2, scdy2 and ldt3-a. by comparing ... | 2017 | 28779232 |
| global distributions and strain diversity of avian infectious bronchitis virus: a review. | the poultry industry faces challenge amidst global food security crisis. infectious bronchitis is one of the most important viral infections that cause huge economic loss to the poultry industry worldwide. the causative agent, infectious bronchitis virus (ibv) is an rna virus with great ability for mutation and recombination; thus, capable of generating new virus strains that are difficult to control. there are many ibv strains found worldwide, including the massachusetts, 4/91, d274, and qx-lik ... | 2017 | 28776490 |
| molecular characterization and phylogenetic analyses of virulent infectious bronchitis viruses isolated from chickens in eastern saudi arabia. | infectious bronchitis virus (ibv) is one of the major respiratory viral threats for chickens. despite the intensive application of ibv vaccines, several outbreaks have been reported worldwide. here, we report several ibv outbreaks in thirteen poultry farms in eastern saudi arabia (esa) from 2013 to 2014. the main goals of the current study were as follows: (1) isolation and molecular characterization of the currently circulating strains in esa (al-hasa, dammam, and buqayq) and (2) evaluation of ... | 2017 | 28770245 |
| infectious bronchitis corona virus establishes productive infection in avian macrophages interfering with selected antimicrobial functions. | infectious bronchitis virus (ibv) causes respiratory disease leading to loss of egg and meat production in chickens. although it is known that macrophage numbers are elevated in the respiratory tract of ibv infected chickens, the role played by macrophages in ibv infection, particularly as a target cell for viral replication, is unknown. in this study, first, we investigated the ability of ibv to establish productive replication in macrophages in lungs and trachea in vivo and in macrophage cell ... | 2017 | 28763472 |
| a robust and cost-effective approach to sequence and analyze complete genomes of small rna viruses. | next-generation sequencing (ngs) allows ultra-deep sequencing of nucleic acids. the use of sequence-independent amplification of viral nucleic acids without utilization of target-specific primers provides advantages over traditional sequencing methods and allows detection of unsuspected variants and co-infecting agents. however, ngs is not widely used for small rna viruses because of incorrectly perceived cost estimates and inefficient utilization of freely available bioinformatics tools. | 2017 | 28388925 |
| infectious bronchitis virus s2 of 4/91 expressed from recombinant virus does not protect against ark-type challenge. | we previously demonstrated that chickens primed with a recombinant newcastle disease virus lasota (rls) expressing the s2 gene of infectious bronchitis virus (ibv) and boosted with an attenuated ibv massachusetts (mass)-type vaccine were protected against ibv arkansas (ark)-type virulent challenge. a possible basis for the reported ability of ibv 4/91 (serotype 793/b) vaccine to protect against divergent ibv strains (e.g., qx, q1, and d1466) in a prime-boost approach with an ibv mass vaccine is ... | 2017 | 28957002 |
| understanding immune resistance to infectious bronchitis using major histocompatibility complex chicken lines. | genetic resistance or susceptibility to infectious diseases has been largely associated with the avian major histocompatibility complex (mhc) genes. our goal was to determine resistance and susceptibility of mhc b haplotype in congenic and inbred chicken lines in order to establish a resistant-susceptible model. eight congenic lines (253/b18, 254/b15, 330/b21, 312/b24, 331/b2, 335/b19, 336/b21, and 342/bo), two inbred lines (003/b17 and 077/b19), and three commercial lines (white leghorn, brown ... | 2017 | 28956996 |
| infectious bronchitis virus infection induces apoptosis during replication in chicken macrophage hd11 cells. | avian infectious bronchitis has caused huge economic losses in the poultry industry. previous studies have reported that infectious bronchitis virus (ibv) infection can produce cytopathic effects (cpe) and apoptosis in some mammalian cells and primary cells. however, there is little research on ibv-induced immune cell apoptosis. in this study, chicken macrophage hd11 cells were established as a cellular model that is permissive to ibv infection. then, ibv-induced apoptosis was observed through a ... | 2017 | 28933760 |
| first characterization of a middle-east gi-23 lineage (var2-like) of infectious bronchitis virus in europe. | variants assigned to gi-23 lineage of infectious bronchitis virus (ibv), formerly called var2, have circulated for nearly 20 years only in countries of the middle east. strains of this lineage were first identified in israel in 1998. more severe form of the virus appeared in 2006, when the second wave of var2 epidemic has spread over the middle east region. the present study describes the detection and detailed genetic characterization of the gi-23 viruses in poland. the full-length genome of ga ... | 2017 | 28923510 |
| protection against infectious bronchitis virus by spike ectodomain subunit vaccine. | the avian coronavirus infectious bronchitis virus (ibv) s1 subunit of the spike (s) glycoprotein mediates viral attachment to host cells and the s2 subunit is responsible for membrane fusion. using ibv arkansas-type (ark) s protein histochemistry, we show that extension of s1 with the s2 ectodomain improves binding to chicken tissues. although the s1 subunit is the major inducer of neutralizing antibodies, vaccination with s1 protein has been shown to confer inadequate protection against challen ... | 2017 | 28899630 |
| molecular characterization of qx-like and variant infectious bronchitis virus strains in malaysia based on partial genomic sequences comprising the s-3a/3b-e-m-intergenic region-5a/5b-n gene order. | infectious bronchitis virus (ibv) is one of the major poultry pathogens of global importance. however, the prevalence of ibv strains in malaysia is poorly characterized. the partial genomic sequences (6.8 kb) comprising the s-3a/3b-e-m-intergenic region-5a/5b-n gene order of 11 malaysian ibvs isolated in 2014 and 2015 were sequenced using next-generation sequencing technology. phylogenetic and pairwise sequence comparison analysis showed that the isolated ibvs are divided into two groups. group ... | 2017 | 29337625 |
| efficacy of massachusetts and 793b vaccines against infectious bronchitis moroccan-italy 02 virus in specific-pathogen-free chickens and commercial broilers. | the ability of commercial vaccines h120 and 4/91 to protect against moroccan-italy 02 infectious bronchitis virus (mor-it02) was investigated in specific-pathogen-free (spf) chickens and commercial broiler chickens. commercial broiler chicks (experiment 1) were vaccinated at the hatchery with h120 vaccine at day 1, and challenged at day 21 with 10450% egg-infective dose (eid50) of mor-it02. all chicks were observed daily for clinical signs attributable to mor-it02 infection during the 10 days po ... | 2017 | 29337615 |
| genotyping and phylogenetic analysis of infectious bronchitis virus isolated from broiler chickens in kashmir. | infectious bronchitis virus (ibv) is responsible for significant economic losses to the poultry industry across the world. the enormous genetic diversity of ibv poses difficulty in diagnosing and controlling the virus. to understand the nature of ibv prevalent in the kashmir himalayas, we characterized two field strains, isolated from non-immunized broiler chickens, by sequence and phylogenetic analysis of s1 subunit of the spike glycoprotein. the analysis revealed both the isolates are identica ... | 2017 | 29291237 |
| comparative transcriptome analysis reveals induction of apoptosis in chicken kidney cells associated with the virulence of nephropathogenic infectious bronchitis virus. | avian infectious bronchitis virus (ibv) that causes respiratory and nephritic diseases in chicken is a major poultry pathogen leading to serious economic loss worldwide. the nephropathogenic ibv strains cause nephritis and kidney lesions intrinsically and the pathogenic mechanism is still unclear. in the present study, spf chicks were infected with three nephropathogenic ibvs of different virulence and their gene expression profiles in chicken kidney were compared at transcriptome level. as a re ... | 2017 | 29174688 |
| astragalus polysaccharides inhibit avian infectious bronchitis virus infection by regulating viral replication. | the avian coronavirus causes infectious bronchitis (ib), which is one of the most serious diseases affecting the avian industry worldwide. however, there are no effective strategies for controlling the ib virus (ibv) at present. therefore, development of novel antiviral treatment strategies is urgently required. as reported, astragalus polysaccharides (aps) have potential antiviral effects against several viruses; however, the antiviral effect of aps against ibv remains unclear. in this study, w ... | 2017 | 29170045 |
| activation of the c-jun nh2-terminal kinase pathway by coronavirus infectious bronchitis virus promotes apoptosis independently of c-jun. | mitogen-activated protein kinases (mapks) are conserved protein kinases that regulate a variety of important cellular signaling pathways. among them, c-jun n-terminal kinases (jnk) are known to be activated by various environmental stresses including virus infections. previously, activation of the jnk pathway has been detected in cells infected with several coronaviruses. however, detailed characterization of the pathway as well as its implication in host-virus interactions has not been fully in ... | 2017 | 29238080 |
| differentiation of infectious bronchitis virus vaccine strains ma5 and 4/91 by taqman real-time pcr. | the aim of this study was to develop rapid molecular assays for differentiating vaccine strains ma5 and 4/91 of the infectious bronchitis virus (ibv). specific primers and probes for s1 and n genes were designed based on the nucleotide sequences of both vaccine strains. cross-reactivity was not observed. assay sensitivity was 2.373 × 103 copies of the ma5 strain, and 3.852 x 103 copies of the 4/91 strain. samples belonging to a known genotype demonstrated that the designed assays supported rapid ... | 2017 | 29166280 |
| molecular characterization of infectious bronchitis viruses isolated from broiler flocks in bushehr province, iran: 2014 - 2015. | the aim of this study was to provide information on the molecular characteristic and the phylogenic relationship of infectious bronchitis viruses (ibv) strains in bushehr province in comparison to other strains reported in the middle east. samples were collected from broiler flocks in bushehr province during 2014 - 2015. these flocks had respiratory problems such as gasping, sneezing and bronchial rales. a number of 135 tracheal swabs were taken from fifteen flocks (nine swabs per flock). each t ... | 2017 | 29085606 |
| reference gene selection for gene expression study in shell gland and spleen of laying hens challenged with infectious bronchitis virus. | ten reference genes were investigated for normalisation of candidate target gene expression data in the shell gland and spleen of laying hens challenged with two strains of infectious bronchitis virus (ibv). data were analysed with genorm, normfinder and bestkeeper, and a comprehensive ranking (geomean) was calculated. in the combined data set of ibv challenged shell gland samples, the comprehensive ranking showed tata-box binding protein (tbp) and tyrosine 3-monooxygenase/tryptophan 5-monooxyge ... | 2017 | 29079779 |
| cd59 association with infectious bronchitis virus particles protects against antibody-dependent complement-mediated lysis. | cd59 protein functions as a negative regulator of the terminal pathway of the complement system by binding to the c8/c9 factors. to date, little is known about the role of cd59 in coronavirus infectious bronchitis virus (ibv) infection. in this study, we discovered that cd59 was downregulated in ibv-infected cells and was associated with ibv virions. this association protected ibv particles from antibody-dependent complement-mediated lysis. ibv titres in the supernatant were significantly increa ... | 2017 | 29068273 |
| comparative genomics of qx-like infectious bronchitis viruses in korea. | to minimize the spread of infectious bronchitis virus (ibv), domestic fowl have been extensively vaccinated with the km91 strain. however, various ibv qx-like virus strains have become increasingly prevalent in korea. we conducted comparative genomic analyses of seven qx-like viruses: early viruses (n = 2), new cluster 1 (nc1; recombinants of km91 and the early qx-like viruses, n = 3) and recurrent viruses (n = 2), to understand their genomic backgrounds. the early and nc1 viruses had km91-like ... | 2017 | 28116527 |
| identification of a novel recombinant virulent avian infectious bronchitis virus. | the emergence of new infectious bronchitis virus (ibv) variants is often disastrous in the poultry industry. in this study, an ibv, ck/ch/2010/jt-1, was isolated from an h120- and 4/91-ibv-vaccinated flock in china. antisera against vaccine strains h120 and 4/91 could not provide effective protection against ck/ch/2010/jt-1 in virus neutralization assays. ck/ch/2010/jt-1 could cause 43.75% mortality with respiratory and severe renal lesions in inoculated chickens. phylogenetic analysis of the s1 ... | 2017 | 28110778 |
| the relationship between liver-kidney impairment and viral load after nephropathogenic infectious bronchitis virus infection in embryonic chickens. | to examine the relationship of impairments of the liver and kidney with viral load after nephropathogenic infectious bronchitis virus (nibv) infection in embryonic chickens, 120 specific-pathogen-free leghorn embryonated chicken eggs were randomly divided into two groups (infected and control), with three replicates per group and 20 eggs in each replicate. the eggs in the infected and control groups were challenged with 0.2 ml of 10(5.5) eld50 nibv and sterile saline solution, respectively. the ... | 2017 | 28108730 |
| a broadly protective therapeutic antibody against influenza b virus with two mechanisms of action. | influenza b virus (ibv) causes annual influenza epidemics around the world. here we use an in vivo plasmablast enrichment technique to isolate a human monoclonal antibody, 46b8 that neutralizes all ibvs tested in vitro and protects mice against lethal challenge of all ibvs tested when administered 72 h post infection. 46b8 demonstrates a superior therapeutic benefit over tamiflu and has an additive antiviral effect in combination with tamiflu. 46b8 binds to a conserved epitope in the vestigial e ... | 2017 | 28102191 |
| vaccination against infectious bronchitis virus: a continuous challenge. | infectious bronchitis virus (ibv) is a significant respiratory pathogen of commercial poultry that causes millions of dollars in lost revenue worldwide each year. even though the poultry industry extensively vaccinates against ibv, emergence of new serotypes and variants continually occur, making control of the disease difficult. current mass application strategies for ibv vaccines are inefficient and frequently result in vaccination failures. novel vaccine technology development has been slow, ... | 2017 | 28081857 |
| the important role of lipid raft-mediated attachment in the infection of cultured cells by coronavirus infectious bronchitis virus beaudette strain. | lipid raft is an important element for the cellular entry of some viruses, including coronavirus infectious bronchitis virus (ibv). however, the exact role of lipid rafts in the cellular membrane during the entry of ibv into host cells is still unknown. in this study, we biochemically fractionated ibv-infected cells via sucrose density gradient centrifugation after depleting plasma membrane cholesterol with methyl-β-cyclodextrin or mevastatin. our results demonstrated that unlike ibv non-structu ... | 2017 | 28081264 |
| origin and evolution of lx4 genotype infectious bronchitis coronavirus in china. | we investigated the genomic characteristics of 110 lx4 genotype strains of infectious bronchitis viruses (ibvs) isolated between 1995 and 2005 in china. the genome of these ibvs varies in size from 27596bp to 27790bp. most ibv strains have the typical genomic organization of other gamacoronaviruses, however, two strains lacked 3a and 5b genes as a result of a nucleotide change within the start codon in the 3a or 5b genes. analysis of our 110 viruses revealed that recombination events may be resp ... | 2017 | 28062013 |
| identification and molecular characterization of a novel serotype infectious bronchitis virus (gi-28) in china. | avian infectious bronchitis coronavirus (ibv) is a major poultry pathogen. a characteristic feature of ibv is the occurrence of many different strains belonging to different serotypes, which renders complete control of the disease by vaccination a challenging task due to the poor cross-protection between different serotypes. in this study, based on the results of s1 sequence analysis and virus cross-neutralization tests, ibv strain ck/ch/lgx/111119 was found to be genetically and antigenically d ... | 2017 | 28062000 |
| channel-inactivating mutations and their revertant mutants in the envelope protein of infectious bronchitis virus. | it has been shown previously in the severe acute respiratory syndrome coronavirus (sars-cov) that two point mutations, n15a and v25f, in the transmembrane domain (tmd) of the envelope (e) protein abolished channel activity and led to in vivo attenuation. pathogenicity was recovered in mutants that also regained e protein channel activity. in particular, v25f was rapidly compensated by changes at multiple v25f-facing tmd residues located on a neighboring monomer, consistent with a recovery of oli ... | 2017 | 27974570 |
| pathogenicity characteristics of an iranian variant-2 (is-1494) like infectious bronchitis virus in experimentally infected spf chickens. | avian infectious bronchitis (ib) is a major cause of economic loss to the poultry industry. ib virus primarily affects respiratory tract, but strains differ in their tropism for such other target organs as kidneys and alimentary tract. the objective of this study was to estimate the pathogenicity of an iranian ib virus (ibv) variant (variant-2) which is one of the most prevalent isolates circulating in iranian poultry farms. spf chickens were intranasally inoculated with 104 eid50/0.1 ml of the ... | 2017 | 27928919 |
| development of an alternative modified live influenza b virus vaccine. | influenza b virus (ibv) is considered a major human pathogen, responsible for seasonal epidemics of acute respiratory illness. two antigenically distinct ibv ha lineages co-circulate worldwide with little cross-reactivity between each other. live attenuated influenza virus vaccines (laiv) have been shown to provide improved cross-protective immune responses compared to inactivated vaccines by eliciting local mucosal immunity and systemic b cell and t cell-mediated memory responses. we have previ ... | 2017 | 28381580 |
| new oligonucleotide microarray for rapid diagnosis of avian viral diseases. | we developed a new oligonucleotide microarray comprising 16 identical subarrays for simultaneous rapid detection of avian viruses: avian influenza virus (aiv), newcastle disease virus (ndv), infection bronchitis virus (ibv), and infectious bursal disease virus (ibdv) in single- and mixed-virus infections. the objective of the study was to develop an oligonucleotide microarray for rapid diagnosis of avian diseases that would be used in the course of mass analysis for routine epidemiological surve ... | 2017 | 28381285 |
| recombinant newcastle disease virus expressing the infectious bronchitis virus s1 gene protects chickens against newcastle disease virus and infectious bronchitis virus challenge. | the recombinant lasota strain expressing a chimeric ibv s1 gene (rlasota-s1) was constructed with the s1 gene of the lx4 type ibv ck/ch/ldl/091022. the expression of the s1 protein was detected by an indirect immunofluorescence assay and western blotting. the rlasota-s1 strain was slightly attenuated, and its growth dynamics were similar to that of the parental lasota strain. vaccination of specific pathogen-free chickens with the rlasota-s1 strain induced ndv hemagglutination inhibition antibod ... | 2017 | 28342665 |
| new real time and conventional rt-pcrs for updated molecular diagnosis of infectious bronchitis virus infection (ibv) in chickens in egypt associated with frequent co-infections with avian influenza and newcastle disease viruses. | in egypt, currently two geographically restricted genotypes of the infectious bronchitis coronavirus (ibv) are circulating with detrimental effects for poultry industry. a sensitive real-time rt-pcr assay targeting the ibv nucleocapsid gene (n) was developed to screen clinical samples for presence of ibv. conventional rt-pcrs amplifying hypervariable regions (hvrs 1-2 and 3) of the ibv s1 gene were developed and amplificates used for nucleotide sequence-based typing of ibv field strains in egypt ... | 2017 | 28336367 |
| the papain-like protease of avian infectious bronchitis virus has deubiquitinating activity. | coronavirus papain-like proteases (plps) can act as proteases that process virus-encoded large replicase polyproteins and also as deubiquitinating (dub) enzymes. like the plps of other coronaviruses (covs), the avian infectious bronchitis virus (ibv) plp catalyzes proteolysis of gly-gly dipeptide bonds to release mature cleavage products. however, the other functions of the ibv plp are not well understood. in this study, we found that ibv exhibits strong global dub activity with significant redu ... | 2017 | 28316013 |
| minimum infectious dose determination of the arkansas delmarva poultry industry infectious bronchitis virus vaccine delivered by hatchery spray cabinet. | the arkansas delmarva poultry industry (arkdpi) infectious bronchitis virus (ibv) vaccine is effective when administered by eye drop, where the vaccine virus is able to infect and replicate well in birds and is able to induce protection against homologous challenge. however, accumulating evidence indicates that the arkdpi vaccine is ineffective when applied by hatchery spray cabinet using the same manufacturer-recommended dose per bird. for this study, we aimed to determine the minimum infectiou ... | 2017 | 28301234 |
| structural basis for dimerization and rna binding of avian infectious bronchitis virus nsp9. | the potential for infection by coronaviruses (covs) has become a serious concern with the recent emergence of middle east respiratory syndrome (mers) and severe acute respiratory syndrome (sars) in the human population. covs encode two large polyproteins, which are then processed into 15-16 nonstructural proteins (nsps) that make significant contributions to viral replication and transcription by assembling the rna replicase complex. among them, nsp9 plays an essential role in viral replication ... | 2017 | 28257598 |
| heat shock protein 70 in lung and kidney of specific-pathogen-free chickens is a receptor-associated protein that interacts with the binding domain of the spike protein of infectious bronchitis virus. | avian infectious bronchitis virus (ibv) is a member of the family coronaviridae. a binding domain that mediates the attachment of the virus to its receptor has been identified in the s1 protein of prototype ibv strain m41. in this study, we identified this binding domain in a different strain, as well as the cellular proteins that interact with it. first, we expressed the s1n proteins (residues 19-270) of m41 and another isolate, sczj3, and compared the binding capacities of recombinant s1n-m41 ... | 2017 | 28224252 |
| early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles. | avian infectious bronchitis virus (ibv) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. however, the association between the local immune responses induced by ibv and the mechanisms of pathogenesis has not yet been completely elucidated. this study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two brazilian field isolates (a and b) of ibv ... | 2017 | 28199419 |
| development and application of nsp5-elisa for the detection of antibody to infectious bronchitis virus. | infectious bronchitis virus (ibv) continues to be one of the most important poultry pathogens worldwide. the current commercially available enzyme-linked immunosorbent assay (elisa) kits for ibv specific antibody detection are mostly based on the whole virion, and few serological tests based on nonstructural proteins of ibv have been developed. herein, an alternative indirect elisa for detection of ibv antibody was developed with ibv nonstructural protein 5 (nsp5) produced by escherichia coli. u ... | 2017 | 28223026 |
| a survey for selected avian viral pathogens in backyard chicken farms in finland. | backyard poultry are regaining popularity in europe and increased interest in the health and management of non-commercial farms has resulted. furthermore, commercial poultry farm owners have become concerned about the risk represented by contagious avian diseases that nearby backyard poultry could transmit. fifty-one voluntary backyard chicken farms were visited between october 2012 and january 2013. blood samples and individual cloacal swabs were collected from 457 chickens. in 44 farms (86%), ... | 2017 | 27624642 |
| maternal antibody decay and antibody-mediated immune responses in chicken pullets fed prebiotics and synbiotics. | three experiments were conducted to evaluate the effect of yeast-derived carbohydrates (ydc), and a blend of probiotics and ydc (synbiotic, snb) on serum igg concentration, maternal-derived antibody (mda) decay, and specific antibody-mediated immune response in chick pullets following immunization with t-cell dependent antigens. a total of 300 day-old pullet chicks were randomly assigned to 3 dietary treatments including: a basal diet (control), and diets containing ydc, and snb (lactobacillus a ... | 2017 | 27433011 |
| potential immunomodulatory effects of non-dialyzable materials of cranberry extract in poultry production. | studies on effects of cranberry products in animals, especially in chickens, are very scarce or even lacking. this study investigated the immunomodulatory effect of high molecular weight non-dialyzable materials (ndms) of cranberry extract. the in vitro antioxidant and anti-inflammatory activities were investigated. the ability of ndms (0, 1, 2, or 4 mg/ml) to enhance phagocytic activities was also evaluated using chicken heterophils (ches) against staphylococcus aureus furthermore, a broiler mo ... | 2017 | 27587728 |
| detection of infectious bronchitis virus 793b, avian metapneumovirus, mycoplasma gallisepticum and mycoplasma synoviae in poultry in ethiopia. | a survey was conducted into respiratory infectious diseases of poultry on a chicken breeder farm run by the ethiopian institute of agricultural research (eiar), located in debre zeit, ethiopia. oropharyngeal swabs were collected from 117 randomly selected birds, and blood was taken from a subset of 73 of these birds. a combination of serological and molecular methods was used for detection of pathogens. for the first time in ethiopia, we report the detection of variant infectious bronchitis viru ... | 2017 | 27924415 |
| a serosurvey of greater sage-grouse ( centrocercus urophasianus ) in nevada, usa. | to better understand the potential avian diseases in greater sage-grouse ( centrocercus urophasianus ) in the great basin in nevada, us, we collected 31 blood samples march-april 2014 and tested for antibodies to eight viruses and two bacteria. specifically, sera were tested for antibodies to avian leukosis virus type a, b, and j (alv-a, alv-b, and alv-j, respectively), infectious bursal disease virus, infectious bronchitis virus, reticuloendothelial virus, avian influenza virus (aiv), west nile ... | 2017 | 27705104 |
| evaluation of the genetic variability found in brazilian commercial vaccines for infectious bronchitis virus. | infectious bronchitis virus (ibv) is currently one of the most important pathogens in the poultry industry. the h120 and ma5 are the only viral strains approved by the brazilian government as the constituent of vaccines. despite the systematic vaccination in brazil, ibv has not yet been controlled and diseases associated with this virus have been reported in vaccinated chickens. here, we investigated the genetic variability of h120 and ma5 strains present in the ibv vaccines from different brazi ... | 2017 | 29128885 |
| complete genome sequence of a novel strain of infectious bronchitis virus, isolated from chickens in china in 2016. | an avian infectious bronchitis virus (ibv) was detected from trachea swabs of chickens in hubei province, china, in 2016. the complete genome of the ibv strain, ck/ch/hb/2016, was characterized and analyzed to better understand ibv epidemiology in china. | 2017 | 29122882 |
| isolation and molecular characterization of novel infectious bronchitis virus variants from vaccinated broiler flocks in egypt. | the present study aimed to determine the molecular characteristics of circulating infectious bronchitis virus (ibv) strains in vaccinated broiler flocks in the giza and fayoum governorates. thirty-four isolates were collected, and egg propagation revealed their ability to induce typical ibv lesions after three to five successive passages. three selected isolates were identified as ibv using a real-time reverse transcriptase-pcr assay targeted the nucleocapsid (n) gene and further characterized b ... | 2017 | 28957013 |
| infectious bronchitis virus variants: molecular analysis and pathogenicity investigation. | infectious bronchitis virus (ibv) variants constantly emerge and pose economic threats to poultry farms worldwide. numerous studies on the molecular and pathogenic characterization of ibv variants have been performed between 2007 and 2017, which we have reviewed herein. we noted that viral genetic mutations and recombination events commonly gave rise to distinct ibv genotypes, serotypes and pathotypes. in addition to characterizing thes1genes, full viral genomic sequencing, comprehensive antigen ... | 2017 | 28937583 |
| think globally, act locally: phylodynamic reconstruction of infectious bronchitis virus (ibv) qx genotype (gi-19 lineage) reveals different population dynamics and spreading patterns when evaluated on different epidemiological scales. | infectious bronchitis virus (ibv) represents one of the poultry industry major threats, particularly in high density producing countries. the emergence and spread of new ibv genotypes have frustrated the various disease control efforts implemented over time. despite that, few comprehensive and large scale studies have been performed to understand the international and local spreading dynamics of this virus. in the present work, these phenomena were evaluated by implementing a bayesian phylodynam ... | 2017 | 28880958 |
| molecular survey of respiratory and immunosuppressive pathogens associated with low pathogenic avian influenza h9n2 subtype and virulent newcastle disease viruses in commercial chicken flocks. | the study was carried out in 48 poultry flocks to elucidate the roles of various complicating pathogens involved along with newcastle disease (nd)/ low pathogenic avian influenza (lpai) outbreaks. necropsy was conducted and samples were collected for the isolation of newcastle disease virus (ndv), influenza a virus, infectious bronchitis virus (ibv), pathogenic bacteria; molecular detection of infectious laryngotracheitis virus (iltv), fowl adeno virus (fav), chicken anaemia virus (cav), mycopla ... | 2017 | 32908424 |
| the first evidence of a new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and unvaccinated broiler flocks in algeria. | avian infectious bronchitis virus (ibv) frequently infects broilers and is responsible for severe economic losses to the poultry industry worldwide. it has also been associated with kidney damage in the broiler flocks. the aim of the present study is to determine the presence of ibv and its possible involvement in kidney damage of broiler chicks. | 2018 | 30587900 |
| gi-16 lineage (624/i or q1), there and back again: the history of one of the major threats for poultry farming of our era. | the genetic variability of infectious bronchitis virus (ibv) is one of the main challenges for its control, hindering not only the development of effective vaccination strategies but also its classification and, consequently, epidemiology understanding. the 624/i and q1 genotypes, now recognized to be part of the gi-16 lineage, represent an excellent example of the practical consequences of ibv molecular epidemiology limited knowledge. in fact, being their common origin unrecognized for a long t ... | 2018 | 30571679 |
| recent approaches for control of e. coli and respiratory complex in middle east. | this study was conducted on 100 one-day-old broiler chicks to evaluate the effect of poulvac e. coli vaccine in reduction of clinical signs and complications after concurrent infectious bronchitis virus (variant 02) and virulent e. coli o78 challenges. the birds were evaluated for clinical signs, mortality for 7 days post-infection, pm lesion score, average body weight and serological evaluation. re-isolation and rt-pcr for the challenging infectious bronchitis virus (ibv) variant 02 were conduc ... | 2018 | 30505174 |
| sensitive sybr green-real time pcr for the detection and quantitation of avian rotavirus a. | avian rotavirus a (artv-a) is a virus that affects young birds, causing acute diarrhea and economic losses in the poultry industry worldwide. the techniques used for the diagnosis of artv-a include electron microscopy, isolation in cell culture, and serology, as well as molecular techniques, such as the reverse transcription-polymerase chain reaction (rt-pcr). the objective of this work was to standardize a real-time rt-polymerase chain reaction (rt-qpcr) using sybr green chemistry for the rapid ... | 2018 | 30597964 |
| combined use of elisa and western blot with recombinant n protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis. | the avian infectious bronchitis virus (ibv) remains a significant source of loss in the poultry industry and early diagnosis is required to prevent the disease from spreading. this study examined the combined use of an elisa and western blot (wb) to detect antibodies against the nucleocapsid protein (n) of ibv. the coding sequence for n was amplified by rt-pcr and expressed in escherichia coli. a soluble recombinant n protein (rn) of approximately 50 kda was obtained. a total of 389 sera were te ... | 2018 | 30541588 |
| comparative features of infections of two massachusetts (mass) infectious bronchitis virus (ibv) variants isolated from western canadian layer flocks. | infectious bronchitis virus (ibv) is one of the leading causes of mortality and morbidity in chickens. there are numerous serotypes and variants, which do not confer cross protection resulting in failure of currently used ibv vaccines. although variant ibv isolates with major genetic differences have been subjected to comparative studies, it is unknown whether minor genetic differences in ibv variants within a serotype are different in terms of pathogenesis and eliciting host responses. two mass ... | 2018 | 30526618 |
| pathogenicity differences between a newly emerged tw-like strain and a prevalent qx-like strain of infectious bronchitis virus. | tw-like ibv isolates have appeared frequently in recent years in mainland china. in this study, we compared the tw-like ibv gd strain and the predominant qx-like sd strain in terms of serology and pathogenicity to 3-week-old specific-pathogen-free chickens. both strains could cause severe respiratory distress and renal lesions, with a mortality rate were approximately 20%. virus were continuously shed via the respiratory tract and cloaca. however, the infection pattern of the two isolates were d ... | 2018 | 30473347 |
| pathobiological and genomic characterization of a cold-adapted infectious bronchitis virus (bp-cakii). | we established a cold-adapted infectious bronchitis virus (bp-cakii) by passaging a field virus through specific pathogen-free embryonated eggs 20 times at 32 °c. we characterized its growth kinetics and pathogenicity in embryonated eggs, and its tropism and persistence in different tissues from chickens; then, we evaluated pathogenicity by using a new premature reproductive tract pathogenicity model. furthermore, we determined the complete genomic sequence of bp-cakii to understand the genetic ... | 2018 | 30463206 |
| the in ovo delivery of cpg oligonucleotides protects against infectious bronchitis with the recruitment of immune cells into the respiratory tract of chickens. | the in ovo delivery of cytosine-guanosine (cpg) oligodeoxynucleotides (odns) protects chickens against many bacterial and viral infections, by activating the toll-like receptor (tlr)21 signaling pathway. although the delivery of cpg odns in ovo at embryo day (ed) 18 has been shown to reduce infectious bronchitis virus (ibv) loads in embryonic chicken lungs pre-hatch, whether in ovo delivered cpg odns are capable of protecting chickens against a post-hatch challenge is unknown. thus, our objectiv ... | 2018 | 30445707 |
| a multiplex xtag assay for the simultaneous detection of five chicken immunosuppressive viruses. | chicken anemia virus (cav), avian reovirus (arv), infectious bursal disease virus (ibdv), marek's disease virus (mdv) and reticuloendotheliosis virus (rev) all cause immunosuppressive disease in birds through vertical or horizontal transmission. mixed infections with these immunosuppressive pathogens lead to atypical clinical signs and obstruct accurate diagnoses and epidemiological investigations. therefore, it is essential to develop a high-throughput assay for the simultaneous detection of th ... | 2018 | 30442149 |
| molecular detection and phylogenetic properties of isolated infectious bronchitis viruses from broilers in ahvaz, southwest iran, based on partial sequences of spike gene. | infectious bronchitis (ib) is a highly contagious disease involving mostly upper respiratory tract in chickens, leading to significant economic losses in the poultry industry worldwide. one of the major concerns regarding to ib is the emergence of new types of infectious bronchitis viruses (ibvs). the purpose of this study was to identify the ibvs isolated from iranian broiler chickens with respiratory symptoms. twenty-five broiler flocks around ahwaz (southwest of iran) were examined for ibv. t ... | 2018 | 30357063 |
| recombinant infectious bronchitis viruses expressing heterologous s1 subunits: potential for a new generation of vaccines that replicate in vero cells. | the spike glycoprotein (s) of infectious bronchitis virus (ibv) comprises two subunits, s1 and s2. we have previously demonstrated that the s2 subunit of the avirulent beau-r strain is responsible for its extended cellular tropism for vero cells. two recombinant infectious bronchitis viruses (ribvs) have been generated; the immunogenic s1 subunit is derived from the ibv vaccine strain, h120, or the virulent field strain, qx, within the genetic background of beau-r. the ribvs beaur-h120(s1) and b ... | 2018 | 30355423 |
| co-circulation of genetically diverse population of vaccine related and unrelated respiratory mycoplasmas and viruses in uk poultry flocks with health or production problems. | respiratory diseases continue to have a major impact on poultry health, welfare and productivity. however, little information is available on their current status in uk poultry flocks. we investigated the presence of four economically important respiratory pathogens in healthy or problematic flocks; infectious bronchitis virus (ibv), avian metapneumovirus (ampv), mycoplasma gallisepticum (mg) and mycoplasma synoviae (ms). samples from 131 uk poultry flocks were received during the 12 month study ... | 2018 | 30322525 |
| sequencing and in silico multi-aspect analysis of s1 glycoprotein in 793/b serotype of infectious bronchitis virus isolated from iran in 2003 and 2011. | infectious bronchitis (ib) is an acute, highly contagious, and economically important viral disease of chickens. the s1 subunit from spike (s) protein plays the major role in protective immunity and is involved in the host-virus interactions, as well as infectious bronchitis virus (ibv) serotyping. aim of the present study was multi-aspect analysis of the molecular and immunological features of 5' part belonging to the s1 glycoprotein sequence of iranian 793/b ibv strain isolates. this m ... | 2018 | 30280838 |
| detection of newcastle disease, h9n2 avian influenza, and infectious bronchitis viruses in respiratory diseases in backyard chickens in ahvaz, iran, in 2014-2015. | newcastle disease virus (ndv), avian influenza virus (aiv), and infectious bronchitis virus (ibv) are the most prevalent viral pathogens in the iranian poultry industry. this study aimed to reveal the presence of these viruses in the backyard chickens in ahvaz, located in the southwest of iran. a total of 100 chickens with respiratory signs and mortality were examined by taking the blood samples as well as tracheal and cloacal swabs. most of the chickens had not received any vaccine. the blood s ... | 2018 | 30256035 |
| burden of exposure to infectious bursal disease virus, infectious bronchitis virus, newcastle disease virus, mycoplasma gallisepticum, and intestinal parasites in introduced broiler chickens on the galapagos. | diseases in introduced broilers can possibly spill over to wild birds on the galapagos. knowledge about the current burden of exposure to pathogens in broilers on the galapagos is very limited. the objective of the study reported here was to measure the burden of exposure to infectious bursal disease virus (ibdv), infectious bronchitis virus (ibv), newcastle disease virus (ndv), mycoplasma gallisepticum (mg), and intestinal parasites in a sample of broiler chickens on 13 farms on santa cruz isla ... | 2018 | 30248128 |
| genetic diversity of avian infectious bronchitis virus in china in recent years. | in this study, 213 infectious bronchitis viruses (ibvs) were isolated from samples collected from 801 flocks suspected to be infected with ibv from january 2016 to december 2017 in china. by using complete nucleotide sequences of s1 gene we determined the phylogeny of these ibv isolates, which in turn allowed us to define six lineages/genotypes, a number of recombinants and a novel variant. the gi-19 lineage was the most frequently isolated type in china in recent years. although scattered mutat ... | 2018 | 30244092 |
| development of a sensitive and specific xmap assay for detection of antibodies against infectious laryngotracheitis and bronchitis viruses. | a serological method to simultaneously detect antibodies against infectious laryngotracheitis virus (iltv) and infectious bronchitis virus (ibv) is imperative for the differential diagnosis and evaluation of antibodies titers after vaccination. | 2018 | 30241540 |
| genetics, antigenicity and virulence properties of three infectious bronchitis viruses isolated from a single tracheal sample in a chicken with respiratory problems. | three different ibv genotypes/serotypes, designated ck/ch/ldl/150434-i (ldl/150434-i), ck/ch/ldl/150434-ii (ldl/150434-ii) and ck/ch/ldl/150434-iii (ldl/150434-iii), were detected in a single tracheal sample from a chicken showing signs of respiratory disease. the viruses were isolated using a cross-neutralization test and limiting dilution in embryonated specific-pathogen-free (spf) eggs. isolate ldl/150434-i was a re-isolation of h120 vaccine strain that was introduced into the chicken flock b ... | 2018 | 30240807 |
| novel protein chip for the detection of antibodies against infectious bronchitis virus. | infectious bronchitis (ib) caused by the ib virus (ibv) can cause acute damage to chickens around the world. therefore, rapid diagnosis and immune status determination are critical for controlling ibv outbreaks. enzyme-linked immunosorbent assays (elisas) have been widely used in the detection of ibv antibodies in the early infection and continuous infection of ib because they are more sensitive and quicker than other diagnostic methods. | 2018 | 30223836 |
| a self-adjuvanted nanoparticle based vaccine against infectious bronchitis virus. | infectious bronchitis virus (ibv) affects poultry respiratory, renal and reproductive systems. currently the efficacy of available live attenuated or killed vaccines against ibv has been challenged. we designed a novel ibv vaccine alternative using a highly innovative platform called self-assembling protein nanoparticle (sapn). in this vaccine, b cell epitopes derived from the second heptad repeat (hr2) region of ibv spike proteins were repetitively presented in its native trimeric conformation. ... | 2018 | 30216376 |
| recombinant infectious bronchitis viruses expressing chimeric spike glycoproteins induce partial protective immunity against homologous challenge despite limited replication in vivo. | vaccination regimes against infectious bronchitis virus (ibv), which are based on a single virus serotype, often induce insufficient levels of cross-protection against serotypes and two or more antigenically diverse vaccines are used in attempt to provide broader protection. amino acid differences in the surface protein, spike (s), in particular the s1 subunit, are associated with poor cross-protection. here, homologous vaccination trials with recombinant ibvs (ribvs), based on the apathogenic s ... | 2018 | 30209177 |
| oral immunization with a novel attenuated salmonella gallinarum encoding infectious bronchitis virus spike protein induces protective immune responses against fowl typhoid and infectious bronchitis in chickens. | fowl typhoid (ft), a septicemic disease caused by salmonella gallinarum (sg), and infectious bronchitis (ib) are two economically important avian diseases that affect poultry industry worldwide. herein, we exploited a live attenuated sg mutant, jol967, to deliver spike (s) protein 1 of ib virus (v) to elicit protective immunity against both ft and ib in chickens. the codon optimized s1 nucleotide sequence was cloned in-frame into a prokaryotic constitutive expression vector, pjhl65. subsequently ... | 2018 | 30208963 |
| comparative pathogenicity of malaysian qx-like and variant infectious bronchitis virus strains in chickens at different age of exposure to the viruses. | infectious bronchitis viruses (ibvs) circulating in malaysia are classified into two groups as malaysian qx-like and variant strains. in this study, the pathogenicity of ibs130/2015 (qx-like) and ibs037a/2014 (variant) ibvs in 1-day-old and 30-day-old specific pathogen free (spf) chickens was characterized. both strains caused respiratory and kidney infections based on immunohistochemistry (ihc), real-time quantitative polymerase chain reaction (qpcr) and a ciliostasis study; however, the result ... | 2018 | 30173857 |
| serological and molecular surveillance of infectious bronchitis virus infection in free-range chickens and guinea fowls in the ga-east district of ghana. | infectious bronchitis is an economically important disease with worldwide distribution. information is available on the presence of infectious bronchitis virus in commercial chicken in parts of ghana but there is no information on free-range poultry and guinea fowls in the country. possible ibv infections among free-range chickens and guinea fowls in abokobi and frafraha communities in the ga-east district of the greater accra region of ghana were investigated using serology and pcr. blood, trac ... | 2018 | 30159336 |
| enhanced pathogenicity of low-pathogenic h9n2 avian influenza virus after vaccination with infectious bronchitis live attenuated vaccine. | in the present study, two experiments were carried out for studying the pathogenicity of h9n2 avian influenza virus (aiv) in broiler chickens after vaccination with different live respiratory viral vaccines. | 2018 | 30147269 |