Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| acute retinal necrosis syndrome. | 1. acute retinal necrosis (arn) represents a specific pattern of clinical presentations for certain herpes virus infections in the posterior segment of the eye. these include varicella zoster virus and herpes simplex virus. 2. patients with arn usually complain of mild to moderate ocular or periorbital pain, foreign body sensation, and a red eye. visual symptoms usually include hazy vision, floaters, and, rarely, decreased peripheral vision. 3. medical treatment of choice is intravenous acyclovi ... | 1992 | 1597865 |
| structural basis of c3b binding by glycoprotein c of herpes simplex virus. | glycoproteins c (gc) from herpes simplex virus type 1 (hsv-1) and hsv-2, gc-1 and gc-2, bind the human complement fragment c3b, although the two glycoproteins differ in their abilities to act as c3b receptors on infected cells and in their effects on the alternative complement pathway. previously, we identified three regions of gc-2 (i, ii, and iii) which are important for c3b binding. in this study, our goal was to identify c3b-binding sites on gc-1 and to continue our analysis of gc-2. we cons ... | 1992 | 1602532 |
| herpes simplex virus type 2 infection and cervical cancer: a prospective study of 12 years of follow-up in finland. | this study was initiated to investigate the role of past herpes simplex virus type 2 (hsv-2) infection, as determined by serum antibody analysis, in the etiology of cervical neoplasia. two finnish registers, the registry of the social insurance institution's mobile clinic survey and the finnish cancer registry, were linked. about 40,000 blood samples were drawn in 1968-72 and stored by the social insurance institution. according to the cancer registry, 32 cases of cervical carcinoma or carcinoma ... | 1992 | 1617120 |
| endemic disease, nutrition and fertility in developing countries. | subfecundity is caused by disease and nutrition as well as by genetic, environmental, and psychological components. sexually transmitted diseases (stds) are caused by 21 different pathogens of which syphilis, gonorrhea, and chlamydia are the most important. syphilis is caused by the bacterium treponema pallidum with incidence of 10% in thailand. 20% in papua new guinea, and 40% in ethiopia. stillbirths in infected mothers range from 66% to 80%. gonorrhea is caused by the bacterium neisseria ... | 1992 | 1634564 |
| herpes simplex virus life cycle and the design of viral vectors. | herpes simplex viruses types 1 and 2 (hsv-1 and hsv-2) are doublestranded dna viruses with a genome size of 152 kbp. the genome consists of two unique regions, u(l) (long) and u(s) (short), flanked by repeated sequences (fig. 1; for review, see ref. 1). the two viruses are closely related, and both infect humans, producing mucocutaneous sores that are predominantly facial in the case of hsv-1 and genital in the case of hsv-2. | 1992 | 21390711 |
| immunogenicity in mice of tandem repeats of an epitope from herpes simplex gd protein when expressed by recombinant adenovirus vectors. | the antigenic and immunogenic potential was examined of human adenovirus type 5 (ad) recombinants carrying and expressing from one to four tandem repeats of a linear neutralizing epitope from the gd protein of herpes simplex virus type 1 (hsv-1) as a fusion with the beta-galactosidase protein. the fusion proteins produced by these ad vectors in infected cell culture reacted with a herpes simplex virus (hsv) epitope-specific monoclonal antibody to a degree dependent on the number of epitope repea ... | 1993 | 7504857 |
| detection of immunoglobulin m antibodies to glycoprotein g-2 by western blot (immunoblot) for diagnosis of initial herpes simplex virus type 2 genital infections. | western blots (immunoblots) for the detection of immunoglobulin m (igm) antibodies specific for herpes simplex virus type 1 (hsv-1) and hsv-2 in patients' sera were developed. the locations of the type-specific glycoprotein g (gpg-2) of hsv-2 (92- and 140-kda forms) and glycoprotein c of hsv-1 (gpc-1), which carries mostly type-specific antigenic epitopes, were checked with specific monoclonal antibodies. western blot assays for igm antibody to gpc-1 or gpg-2 were performed after depletion of ig ... | 1993 | 7508453 |
| ms strain of type 2 herpes simplex virus produces necrotizing retinitis in mice. | intracerebral inoculation of mice with the ms strain of type 2 herpes simplex virus (hsv-2) causes a brief encephalitis associated with multifocal central nervous system demyelination. many of the mice develop unilateral or bilateral impairment of the pupillary light reflex. we have examined the development of ocular disease in inbred nih mice inoculated intracerebrally with a low dose (10 pfu) of hsv-2 (ms). the resulting acute encephalitis was fatal in 30-50% of the mice. by 1 month after inoc ... | 1993 | 7683330 |
| biological response modifier-mediated resistance to herpesvirus infections requires induction of alpha/beta interferon. | the role of interferon alpha/beta (ifn) induction by the immunomodulators piclc and cl246,738 was investigated in cd-1 mice infected with murine cytomegalovirus (mcmv) or herpes simplex virus type 2 (hsv-2). mice were treated with either normal sheep serum or anti-alpha/beta ifn antiserum, inoculated with the immunomodulators, and infected with virus. because anti-ifn treatment also decreased natural resistance to hsv-2 and mcmv, two viral challenge doses were used to ensure that the mice with c ... | 1993 | 7687839 |
| antibodies to epitopes of herpes simplex virus type 1 glycoprotein b (gb) in human sera: analysis of functional gb epitopes defined by inhibition of murine monoclonal antibodies. | the epitopes on herpes simplex virus (hsv) glycoprotein b (gb) recognized by sera of 23 patients with well-characterized hsv infection were studied. twelve epitope-specific monoclonal antibodies with neutralization (nt) or antibody-dependent cellular cytotoxicity (adcc) activities were used in competitive elisa binding inhibition studies. the sera were additionally analyzed for homologous viral type nt, adcc activity, and gb-reactive antibody by elisa. seroconversion was observed in each assay d ... | 1993 | 7690824 |
| the inhibitory effect of spermicidal agents on replication of hsv-2 and hiv-1 in-vitro. | five spermicides including nonoxynol-9 were assessed under in-vitro conditions, for their inhibitory activity against two viruses capable of spread by sexual intercourse, herpes simplex virus type-2 (hsv-2) and the human immunodeficiency virus type-1. a further eight commercially-available spermicidal preparations containing varying concentrations of either nonoxynol-9 or nonoxynol-11 were also assessed for activity against hsv-2. all spermicides and spermicidal preparations tested showed inhibi ... | 1993 | 7693643 |
| identification of renal sympathetic preganglionic neurons in hamsters using transsynaptic transport of herpes simplex type 1 virus. | herpes viruses have been used as retrograde transsynaptic tracers to identify pathways from the cns to specific target tissues. we used herpes simplex virus to identify central nervous system neurons responsible for control of the kidney. herpes simplex type 1 or herpes simplex type 2 was injected into rat kidneys and herpes simplex type 1 was microinjected into hamster and guinea pig kidneys. after three to seven days, ganglia, spinal cords and brains were examined using immunohistochemistry to ... | 1993 | 7694186 |
| bleomycin differentially inhibits picornavirus, herpesvirus and poxvirus replication in a human carcinoma cell line. | ms-757, a cervix carcinoma cell line, was exposed to bleomycin at concentrations up to 200 microm for periods of 1 to 24 hours. bleomycin treatment caused the level of dna synthesis in uninfected cells to drop to 13% of the level achieved in the controls. protein synthesis fell to 50%, but rna synthesis was not affected. exposure of uninfected cells to 200 microm bleomycin for 24 h did not induce significant cell death measured as permeability to trypan blue). a tetrazolium dye-reduction assay, ... | 1993 | 7514894 |
| [recurrent cutaneous herpes in the newborn and acyclovir]. | the authors report two cases of cutaneous recurrent herpes occurring after a neonatal herpes simplex virus type 2 (hsv2) infection and comment on the role of acute or suppressive therapy by aciclovir (acv). the two infants were not treated by acv after the neonatal period. none of the recurrent cutaneous herpes episodes was followed by viral widespread. one case reported by bergström et al on a relapse of hsv2 encephalitis occurring after a cutaneous herpes in a child argues for the use of acv i ... | 1993 | 7777392 |
| [antiviral activity of mangiferin against herpes simplex virus type 2 in vitro]. | the effect of mangiferin, a tetrahydroxy pyrrolidone saponin extracted from the leaves of mango (mangifera indica), against herpes simplex virus type 2 (hsv-2) in vitro was studied. the 50% effective concentration (ec50) of it against hsv-2 plaque formation in hela cells was 111.7 micrograms.ml-1, and the concentrations of 33 and 80 micrograms.ml-1 reduced the virus replicative yields by 90% (ec90) and 99% (ec99), respectively. the therapeutic index (ic50/ec50) was 8.1. mangiferin did not direct ... | 1993 | 8010041 |
| analysis of the epidemiology and pathogenesis of herpes simplex virus (hsv) infections in pregnant women and infants using the hsv-2 glycoprotein g antibody assay. | 1993 | 8012738 | |
| [the primary immune response and virus-specific and -nonspecific immune complexes in neonatal cytomegalovirus and herpetic infection]. | 1993 | 8079522 | |
| epidemiology of genital herpes infections. | much has been learned in the last decade about the epidemiology of genital herpes infections, including new information about seroprevalence and the risk of transmission of genital herpes to sex partners and at delivery. unfortunately, the type-specific serologic assays now routinely used in these studies are not widely available, and commercially available assays that claim to be type-specific are not. thus, most clinicians still do not have access to reliable type-specific assays. in cross-sec ... | 1993 | 8106731 |
| new compounds related to podophyllotoxin and congeners: synthesis, structure elucidation and biological testing. | 4-azido, 4-amino, 4-amido and 4-alkoxy compounds related to the lignans podophyllotoxin and 4'-demethylepipodophyllotoxin have been synthesized, and their structures elucidated. the ritter reaction was shown to be useful in the preparation of the 4-amido compounds with the required stereochemistry. a preparative method for 4-chloro-4-deoxypicrophyllotoxin, for which all earlier synthetic attempts resulted in the two dehydrated compounds, alpha- and beta-apopicropodophyllotoxin, was developed. su ... | 1993 | 8110531 |
| disseminated herpes simplex virus infection during pregnancy. a case report. | hepatitis due to herpes simplex virus (hsv) developed in a pregnant women at 38 weeks' gestation. she delivered a live-born infant who had serologically documented hsv 2 infection but did well with acyclovir therapy. the mother, however, died five days postpartum from fulminant hepatic failure despite antiviral treatment, and hsv was demonstrated in the liver. twenty-three reported cases clearly establish pregnancy as a condition that can predispose to disseminated hsv infection. the majority of ... | 1993 | 8120855 |
| neonatal herpes policy in the netherlands. five years after a consensus conference. | in order to establish whether the recommendations of an in 1987 organized consensus conference about neonatal herpes policy in the netherlands had been followed, an inquiry was held in january 1992 among gynecologists, pediatricians and microbiologists. compared with the results of an inquiry that was held five years before, it was found that the incidence of neonatal herpes had not increased in the last ten years (approximately five cases annually or one per 35,000 neonates) despite the lower f ... | 1993 | 8126633 |
| antiviral activity of nerve growth factor in vitro. | the antiviral activity of 'nerve growth factor' (ngf) on non-oncogenic dna, on rna viruses and on moloney sarcoma retrovirus was evaluated in vitro. ngf was active against herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2) and moloney sarcoma virus (msv) at non toxic concentrations. the effects of different treatment regimens on hsv-1 infections indicate that the inhibitory action of ngf occurs at the early stages of viral replication. no activity was noted against coxsacki ... | 1993 | 8131710 |
| [ileocolitis caused by herpes simplex virus type 2]. | the authors report the case of a 51-year-old woman who was hospitalized for severe acute colitis. sub-total colectomy was performed, due to clinical deterioration and resistance to treatment. the examination of the surgery specimen revealed a herpes-virus type 2 as the responsible pathogen agent. complementary aciclovir treatment cured the patient. | 1993 | 8143954 |
| the herpes simplex virus type 2 ul3 open reading frame encodes a nuclear localizing phosphoprotein. | the herpes simplex virus type 2 (hsv-2, strain 333) ul3 open reading frame (orf) codes for a protein with a predicted molecular weight of 29,681. comparisons of the ul3, strain 333 orf show essentially complete amino acid identity with hsv-2 (strain hg52) and a 75% amino acid identity with hsv type 1 (strain 17). to characterize the expression of this gene, a hydrophilic region of the hsv-2 ul3 gene was cloned into a bacterial expression vector. the resulting fusion protein was used to generate ... | 1993 | 8337818 |
| detection of herpesviruses by polymerase chain reaction in lymphocytes from patients with rheumatoid arthritis. | to investigate the occurrence of herpesviruses, including epstein-barr virus (ebv), herpes simplex viruses types 1 and 2 (hsv-1; hsv-2), and human herpesvirus 6 (hhv-6), in lymphocytes from patients with rheumatoid arthritis (ra) of less than 1 year's duration. | 1993 | 8343184 |
| mucosal immunity and protection after intranasal immunization with recombinant adenovirus expressing herpes simplex virus glycoprotein b. | a recombinant adenovirus (ad) expressing glycoprotein b (gb) of herpes simplex virus (hsv) type 1 (adgb8) was evaluated as a mucosal vaccine candidate. when administered intranasally (inl) to c57b1/6 mice, adgb8 induced levels of serum anti-hsv gb igg antibodies similar to those of mice immunized intraperitoneally (ip), which neutralized both hsv-1 and -2. mice immunized inl with adgb8 produced secretory iga specific for hsv gb, but mice immunized ip did not. splenic anti-hsv cytotoxic t lymphoc ... | 1993 | 8354903 |
| human papillomaviruses and other influences on survival from cervical cancer in panama. | to determine the influence on survival from cervical cancer of human papillomaviruses (hpvs) and other factors including age, herpes simplex virus type 2 (hsv-2) antibody status, and number of pregnancies. | 1993 | 8380103 |
| [demonstration of herpes simplex virus (hsv)-2 igg and igm using elisa in transsexuals and homosexuals]. | in this study, the sera of 12 cases of transsexual with vaginoplasty and 15 cases of homosexual have been investigated with elisa from the view point of hsv-2 igg and igm antibodies. twenty-five cases were within the 18-26 age groups and the other 2 transsexuals were 36 years old. our studying group have no significant clinical pathology. in all the 27 cases of transsexual and homosexual have been found to be positive for hsv-2 igg. in 7 cases, hsv-2 igm have been found positive, the other 20 ca ... | 1993 | 8380622 |
| herpes laryngitis and tracheitis causing respiratory distress in a neonate. | a newborn presented 1 week after birth with the sudden onset of stridor and respiratory distress. at endoscopy, the supraglottic and subglottic tissues were edematous and erythematous, and numerous plaquelike ulcers were seen on the mucosal surface. cultures of the lesions yielded herpes simplex virus type 2. the infant required a prolonged course of acyclovir and remained intubated for 31 days, but ultimately had both virological and clinical cure. evaluation and further treatment of this unusu ... | 1993 | 8381288 |
| rapid detection of herpes simplex virus in culture by in situ hybridization. | detection of herpes simplex virus (hsv) by a newly developed hsv-dna in situ hybridization (ish) technique (diagnostic hybrids, inc., athens, oh, usa) was compared with a reference standard that combines observation for cytopathic effect in shell vial cultures with subsequent identification of virus by staining with fluorescein-labeled hsv-specific monoclonal antibody. the new technique utilizes a probe consisting of an alkaline phosphatase direct labeled, cloned, single stranded dna fragment th ... | 1993 | 8381792 |
| hla-unrestricted killing of hsv-1-infected mononuclear cells. involvement of either gamma/delta+ or alpha/beta+ human cytotoxic t lymphocytes. | the characterization of hsv-specific human ctl, obtained in short term cultures by stimulating pbmc of healthy hsv-immune donors with autologous, pha-activated, hsv-1-infected mononuclear cells, is described. ctl induced by using this technique are able to mediate a strong lytic activity against both hsv-1- and hsv-2-infected targets, whereas they do not kill autologous ebv-lymphoblastoid cell lines unless they are superinfected with hsv-1. tcr-gamma/delta+ cells are mainly responsible for hsv-s ... | 1993 | 8381835 |
| adjuvant effects of imiquimod on a herpes simplex virus type 2 glycoprotein vaccine in guinea pigs. | the adjuvant effects of imiquimod for a herpes simplex virus (hsv) vaccine were evaluated. guinea pigs were immunized with 35 micrograms of lectin-purified hsv-2 glycoproteins 14 and 35 days before intravaginal hsv-2 inoculation. immunizations were given either alone, with complete freund's adjuvant, or with three different 5-day regimens of imiquimod. although immunization alone decreased the severity of the acute disease and viral replication, in two separate experiments, the addition of imiqu ... | 1993 | 8382722 |
| herpes simplex virus infection of human fibroblasts and keratinocytes inhibits recognition by cloned cd8+ cytotoxic t lymphocytes. | cd8+ cytotoxic t lymphocytes (ctl) clones with specificity for herpes simplex virus (hsv) were derived from two donors with genital hsv-2 infection. these ctl clones specifically lysed hsv-infected autologous b lymphoblastoid cells, but not hsv-infected fibroblasts. exogenous peptide loading sensitized both cell types to lysis by an hsv-specific ctl clone of known specificity. hsv infection rendered fibroblasts refractory to peptide sensitization. hsv infection also rendered fibroblasts and kera ... | 1993 | 8383706 |
| risk of acquisition of genital herpes simplex virus type 2 in sex partners of persons with genital herpes: a prospective couple study. | in a prospective study of factors associated with genital herpes simplex virus (hsv) type 2 transmission in couples in whom 1 partner had clinical genital hsv and the other did not, 11 (19%) of the 57 history-negative partners had hsv-2 antibody by western blot at entry. in follow-up (mean, 16 months) of 29 hsv-2-seronegative partners, overall 4 (14%) seroconverted compared with 3 (23%) of 13 hsv-1- and -2-seronegative partners and 1 (6%) of 16 hsv-1-seropositive partners. since all seroconverte ... | 1993 | 8383724 |
| differential mechanism of cytostatic effect of (e)-5-(2-bromovinyl)-2'-deoxyuridine, 9-(1,3-dihydroxy-2-propoxymethyl)guanine, and other antiherpetic drugs on tumor cells transfected by the thymidine kinase gene of herpes simplex virus type 1 or type 2. | after they have been transfected with the herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) thymidine kinase (tk) gene murine mammary carcinoma (fm3a) cells become highly sensitive to the growth inhibitory properties of the antiherpetic agents (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu), 9(-)[(2-hydroxyethoxy)methyl]guanine (acyclovir, acv), 9(-)[(1,3-dihydroxy-2-propoxy)methyl]guanine (dhpg, ganciclovir), and 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau). bvdu was 100-f ... | 1993 | 8384209 |
| the pseudorabies virus host-shutoff homolog gene: nucleotide sequence and comparison with alphaherpesvirus protein counterparts. | the virion host shutoff function (vhs) of many herpesviruses is required for inhibition of cellular gene expression in infected cells. this function corresponds to the ul41 open reading frame of herpes simplex virus type 1 (hsv-1) and homolog sequences have been found in other alphaherpesvirus, like hsv-2, varicella-zoster virus (vzv) and equine herpesvirus type 1 (ehv-1). in this work, we have cloned and sequenced a pseudorabies virus (prv) gene which is homologous to the vhs genes of the other ... | 1993 | 8384744 |
| antiviral effect of oligo(nucleoside methylphosphonates) complementary to the herpes simplex virus type 1 immediate early mrnas 4 and 5. | we have previously shown that an oligo(nucleoside methylphosphonate) (deoxynucleoside methylphosphonate residues in italics) complementary to the acceptor splice junction of herpes simplex virus type 1 (hsv-1) immediate-early (ie) pre-mrnas 4,5 [d(tptcctcctgcgg)], causes sequence-specific inhibition of virus growth in infected cell cultures (smith et al., 1986; kulka et al., 1989). here we report a similar inhibition of hsv-1 growth by oligo(nucleoside methylphosphonates) complementary to the sp ... | 1993 | 8384823 |
| encephalitis in immunocompetent patients due to herpes simplex virus type 1 or 2 as determined by type-specific polymerase chain reaction and antibody assays of cerebrospinal fluid. | a herpes simplex virus type 2 (hsv 2) etiology was sought in 93 consecutive cases of herpes simplex encephalitis (hse) in immunocompetent post neonate patients. antibodies to hsv 2 glycoprotein g antigen were determined by an enzyme-linked immunosorbent assay (elisa) and hsv 2 dna in cerebrospinal fluid (csf) by a nested polymerase chain reaction (pcr) assay with primer pairs in the glycoprotein g gene. evidence of hsv 2 infection was found in 6 patients; hsv 2 dna was demonstrated in csf and th ... | 1993 | 8385702 |
| baculovirus-expressed herpes simplex virus type 2 glycoprotein d is immunogenic and protective against lethal hsv challenge. | herpes simplex virus type 2 glycoprotein d (gd2) was cloned and expressed in the baculovirus-spodoptera frugiperda system. milligram quantities of glycoprotein were recovered from suspension culture and subjected to purification by ion-exchange and immunoaffinity chromatography. the resultant purified gd existed as a homogeneous 57,500 mw monomeric species demonstrating reactivity with anti-gd monoclonal antibodies including those directed at a non-sequential neutralizing epitope of gd. immuniza ... | 1993 | 8385842 |
| sequence analysis of herpes simplex virus gb gene homologs of two platyrrhine monkey alpha-herpesviruses. | homologs of the herpes simplex virus gb gene were identified in two alpha-herpesviruses of platyrrhine monkeys, herpesvirus saimiri 1 (hvs 1) and h. ateles 1 (hva 1). these genes were cloned and sequenced in their entirety. analysis of the predicted amino acid sequences indicated that the gb glycoproteins of these two viruses are of similar size, have 10 cys residues and 5 potential n-linked glycosylation sites which align exactly with those in other primate alpha-herpesvirus gb polypeptides, an ... | 1993 | 8385913 |
| nucleotide sequence of the major dna-binding protein gene of herpes simplex virus type 2 and a comparison with the type 1. | the nucleotide sequence of a region encompassing about 5,200 base pairs (bp) of the left side of the origin of replication in the long unique region of the herpes simplex virus type 2 (hsv-2) has been determined. this region contained the major dna-binding protein or the infected-cell protein 8 (icp 8) gene and 5'-part of the counterpart of hsv-1 icp 18.5 gene. a comparison of the nucleotide sequence of the icp8 gene between hsv-1 and hsv-2 showed an 89.8% homology. a primer extension analysis f ... | 1993 | 8385914 |
| analysis of a herpes simplex virus 2 fragment from the open reading frame of the large subunit of ribonucleotide reductase with transcriptional regulatory activity. | expression of herpes simplex virus 2 (hsv-2)-encoded ribonucleotide reductase (rr) is required for growth in nondividing cells. the functional enzyme is composed of a large and a small subunit. in virus-infected cells, rr is expressed temporally as a delayed early protein. however, the promoter regulatory region of the large subunit can function as an immediate early promoter in transient transfection assays, suggesting that expression may be quite complex. in this study, a 95-bp fragment derive ... | 1993 | 8385955 |
| human papillomavirus, herpes simplex virus and other potential risk factors for cervical cancer in a high-risk area (greenland) and a low-risk area (denmark)--a second look. | the prevalence of human papillomavirus (hpv) infection and other risk factors were studied in a high risk area for cervical cancer (greenland) and in a low risk area (denmark). from nuuk (greenland) and nykøbing falster (denmark), random samples of 150 women aged 20-39 years were drawn. a total of 129 and 126 women were included in greenland and denmark, respectively. the proportion of hpv infected women assessed by virapap was similar in denmark and greenland (4.8 vs 3.9%). when type specific p ... | 1993 | 8385982 |
| antiviral properties of the seed extract of an indian medicinal plant, pongamia pinnata, linn., against herpes simplex viruses: in-vitro studies on vero cells. | pongamia pinnata, linn., an indian medicinal plant used in the ayurvedha and siddha traditional medicine systems, for treatment of clinical lesions of skin and genitalia, was evaluated for antiviral properties against herpes simplex virus type-1 (hsv-1) and type-2 (hsv-2) by in-vitro studies in vero cells. a crude aqueous seed extract of p. pinnata completely inhibited the growth of hsv-1 and hsv-2 at concentrations of 1 and 20 mg/ml (w/v), respectively, as shown by complete absence of cytopathi ... | 1993 | 8386251 |
| resistance of herpes simplex virus type 2 to neomycin maps to the n-terminal portion of glycoprotein c. | entry of herpes simplex virus (hsv) into cells is believed to be mediated by specific binding of envelope proteins to a cellular receptor. neomycin specifically blocks this initial step in infection by hsv-1 but not hsv-2. resistance of hsv-2 to this compound maps to a region of the genome encoding glycoprotein c (gc-2). we have studied the function of gc-2 in the initial interaction of the virus with the host cell, using hsv-2 mutants deleted for gc-2 and gc-2-rescued recombinants. resistance t ... | 1993 | 8386261 |
| orientation of the cleavage site of the herpes simplex virus glycoprotein g-2. | during the synthesis of glycoprotein g-2 (gg-2) of herpes simplex virus type 2, the 104,000-da gg-2 precursor (104k precursor) is cleaved to generate the 72k and the 31k intermediates. the 72k product is processed to generate the mature gg-2 (molecular mass, 108,000 da), while the 31k product is additionally processed and secreted into the extracellular medium as the 34k component (h. k. su, r. eberle, and r. j. courtney, j. virol. 61:1735-1737, 1987). in this study, the orientations of the 31k ... | 1993 | 8386284 |
| the proportion of herpes simplex virus-specific cytotoxic t lymphocytes (tc) that recognize glycoprotein c varies between individual mice and is dependent on the form of immunization. | in mice the immune response to hsv-1 includes a brisk tc response that is intimately associated with the control of infection. this report evaluated the tc response to gc, one of the envelope glycoproteins of hsv-1. this protein was recognized as a target antigen for tc from hsv-1 immune mice only if they expressed the h-2kb mhc allele. however, even within these "responder" strains of mice the proportion of gc specific tc was highly variable. the failure of hsv-induced tc to recognize gc in the ... | 1993 | 8386515 |
| efficacy of the herpes simplex virus types 1 and 2 mutant viruses to confer protection against zosteriform spread in mice. | two mutant viruses, hsv-2 xd192 and hsv-1 1716, failed to generate zosteriform lesions when injected in high dose into balb/c and c3h mice. mice exposed to mutant viruses were solidly immune to challenge by wild-type homologous or heterologous virus. however, at lower immunizing doses protection was evident against lethality, but not skin lesions, especially in the case of mutant xd192. protection could be conferred with lymphoid cells from mutant virus immune mice and again, protection against ... | 1993 | 8386516 |
| herpes simplex virus type-2 infection by a footpad route results in neuronal death in mouse spinal ganglia. | we have examined the effects of herpes simplex virus type 2 (hsv-2) infection on neuron numbers in mouse dorsal root ganglia (drg) following unilateral hind footpad inoculation. one month following hsv-2 strain ms inoculation, tissue sections of decalcified spine containing the paired 4th and 5th lumbar drgs were stained with cresyl violet. neuronal numbers, somal areas and ganglion volumes were determined for ganglia ipsilateral and contralateral to both hsv-2 and medium inoculations. one month ... | 1993 | 8387099 |
| in vitro antiviral activity of polyoxomolybdates. mechanism of inhibitory effect of pm-104 (nh4)12h2(eu4(moo4)(h2o)16(mo7o24)4).13h2o on human immunodeficiency virus type 1. | a screening for inhibitors of human immunodeficiency virus type 1 (hiv-1) among various types of isopolyoxomolybdates and heteropolyoxomolybdates was carried out by using an in vitro assay system measuring the cytopathogenicity of hiv-1 in cd4+ human mt-4 cells. a novel heteropolyoxomolybdate named pm-104 with the chemical formula (nh4)12h2(eu4(moo4)(h2o)16(mo7o24)4).13h2o was found to be associated with potent anti-hiv-1 activity. pm-104 interferes with virus infection at a very early step such ... | 1993 | 8387260 |
| induction and enhancement of immune responses to herpes simplex virus type 2 in humans by use of a recombinant glycoprotein d vaccine. | a vaccine for a chronic or recurrent viral infection should induce immune responses that protect against primary disease or that augment preexisting defenses sufficiently to diminish the likelihood of disease recurrence or progression. such a vaccine was sought for genital herpes, a sexually transmitted infection of epidemic proportion. vaccine containing recombinant herpes simplex virus type 2 glycoprotein d expressed in cho cells was given repeatedly and safely to 24 human volunteers. in previ ... | 1993 | 8387560 |
| synthesis and antiviral activity of novel isonucleoside analogs. | a series of branched-chain sugar isonucleosides was synthesized and evaluated for antiviral activity against herpesviruses. the preparation of homochiral [3s-(3 alpha, 4 beta, 5 alpha)]-2-amino-1, 9-dihydro-9-[tetrahydro-4,5-bis(hydroxymethyl)-3-furanyl]-6h-purin-6-one (7, bms-181,164) and related compounds was stereospecifically achieved starting from 1,2-isopropylidene-d-xylofuranose (10). an efficient two-step reduction of the anomeric center of bis-acetate 18 involved formation of the chlori ... | 1993 | 8387600 |
| herpes simplex virus type 2 antibody in patients attending antenatal or std clinics. | to determine the prevalence of antibody to herpes simplex virus type 2 (hsv-2) in patients attending a general public antenatal clinic and three public sexually transmitted disease (std) clinics in sydney. | 1993 | 8387628 |
| kinetic studies of the predicted substrate-binding site of varicella-zoster virus thymidine kinase. | to investigate the mechanism of kinetic action and substrate recognition of varicella-zoster virus (vzv) thymidine kinase (tk), we designed and isolated a site-directed mutant vzv tk which has double amino acid substitutions, 136threonine to leucine and 137isoleucine to leucine (sdm tk). this mutant was designed to alter the substrate-binding site of the vzv tk to duplicate that of the herpes simplex virus type 2 enzyme. kinetic studies of the activity of wild-type tk indicated that the binding ... | 1993 | 8389797 |
| characterization of human keratinocytes transformed by high risk human papillomavirus types 16 or 18 and herpes simplex virus type 2. | recent reports implicate two dna tumour viruses, herpes simplex virus type 2 (hsv-2) and human papillomavirus types 16 or 18 (hpv-16 and -18), in the pathogenesis of cervical cancer. previous studies have indicated that primary human fibroblasts transfected with hpv-16 and hsv-2 morphological transforming region iii (mtr iii) are more aneuploid than fibroblasts immortalized with hpv-16 and that hsv-2 dna sequences are retained in transformed cells. since hsv-2 and hpv typically infect cells of e ... | 1993 | 8389810 |
| guanylylation and adenylylation of the alpha regulatory proteins of herpes simplex virus require a viral beta or gamma function. | herpes simplex virus genes form several groups whose expression is coordinately regulated and sequentially ordered in a cascade fashion. most of the products of the first group, the alpha genes, appear to have regulatory functions. we report that the alpha proteins, infected cell proteins 4, 0, 22, and 27 of herpes simplex virus 1 and 4, 0, and 27 of herpes simplex virus 2, were labeled in the isolated nuclei of infected hela cells with [alpha-32p]gtp or [alpha-32p]atp late in infection and that ... | 1993 | 8389911 |
| herpes simplex virus infection in pregnancy. | genital infections in pregnancy caused by herpes simplex virus types 1 and 2 are a difficult management problem. both primary and recurrent genital herpes can range from severe, extensive ulceration to asymptomatic virus shedding. although neonatal herpes is a well recognized complication of symptomatic maternal primary genital infection at the time of delivery, most cases are associated with asymptomatic virus shedding and absence of a history of genital herpes. neonatal herpes may also be acqu ... | 1993 | 8390339 |
| oral acyclovir and recurrent genital herpes during late pregnancy. | to assess plasma acyclovir levels in pregnant women given oral acyclovir during late gestation and to determine the role and effect of oral acyclovir on asymptomatic shedding of virus in cases of recurrent genital herpes. | 1993 | 8390630 |
| herpesvirus infections of the cns: mr findings. | herpesvirus infections of the cns are prevalent in all segments of the population, and are an important health care issue. the family of herpesviruses consists of a large group of double-stranded dna viruses that includes herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2), cytomegalovirus, epstein-barr virus, varicella-zoster virus, b virus, herpesvirus 6, and herpesvirus 7. in addition to producing infection when the host initially acquires the virus, an important property ... | 1993 | 8390790 |
| the characteristic which makes the cell-coded hsv-inducible u90 distinctive in transformed cells is its greatly increased half-life. | a set of polypeptides detected in transformed cells of m(r) values 90,000 (a doublet) 40,000 and 32,000 that are recognised by immunoprecipitation of l-[35s]methionine-labelled tumour cell lysates, with sera from tumour-bearing rats and with antisera raised against herpes simplex virus type 2 (hsv-2)-infected cells, were previously reported (macnab et al., 1985, 1992; hewitt et al., 1991). these proteins are cell-coded and cannot be precipitated from similarly radiolabelled control cells. two of ... | 1993 | 8391013 |
| phosphorylation of neurotropic alphaherpesvirus envelope glycoproteins: herpes simplex virus type 2 ge2 and pseudorabies virus gi. | i have examined the state of phosphorylation of the envelope glycoproteins of two neurotropic herpesviruses, hsv-2 and prv. hsv-2 ge2 and prv gi, which are the homologues to hsv-1 ge, were found to be phosphorylated and phosphoaminoacid analysis revealed that both contained phosphoserine. these findings are consistent with a conserved phosphorylation of the glycoprotein homologues to hsv ge among the neurotropic alphaherpesviruses. | 1993 | 8391184 |
| evaluation of hpmpc therapy for primary and recurrent genital herpes in mice and guinea pigs. | the nucleoside analogue (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (hpmpc) inhibited the replication of herpes simplex virus (hsv) types 1 and 2 in tissue culture cells at about 1.0 micrograms/ml, whereas acyclovir (acv) had an ec50 of about 0.10-0.50 micrograms/ml. the purpose of these studies was to evaluate the efficacy of topically applied hpmpc in animal models of primary and recurrent genital hsv-2 infections. mice treated with 5%, 1% or 0.5% hpmpc three times daily, beginning 6 o ... | 1993 | 8391249 |
| passive transfer of anti-herpes simplex virus type 2 monoclonal and polyclonal antibodies protect against herpes simplex virus type 1-induced but not herpes simplex virus type 2-induced stromal keratitis. | to investigate whether passive transfer of antibodies to viral glycoproteins would protect against herpes simplex virus type 2-induced stromal keratitis. | 1993 | 8392037 |
| molecular analysis of a neurovirulent herpes simplex virus type 2 strain with reduced thymidine kinase activity. | thymidine kinase (tk) of herpes simplex virus (hsv) has been identified as one of the factors responsible for its virulence. we have previously isolated acyclovir (acv)-resistant hsv type 2 (hsv-2), strain ys-4 c-1, by simple plaque cloning from a clinical isolate. although ys-4 c-1 had extremely low tk activity, it retained high virulence in mice. to determine the mechanism of the reduction of tk activity, a molecular analysis of the ys-4 c-1 tk gene was performed. ys-4 c-1 produced tk mrna, wh ... | 1993 | 8392322 |
| differentiation of herpes simplex virus types 1 and 2 by plaque appearances on semicontinuous rabbit lens epithelial cells in the clinical laboratory. | herpes simplex virus type 1 (hsv1) and type 2 (hsv2) were differentiated on the basis of different plaque appearance on semicontinuous rabbit lens epithelial (rle) cells. plaques produced by hsv1 strains were small; the mean diameter was 1.29 +/- 0.37 mm 3 days after inoculation. hsv2 strains produced large and small plaques, with the ratio of large to small about 20:1. the mean diameters of the large and the small plaques of hsv2 were 3.34 +/- 0.56 mm and 0.97 +/- 0.31 mm respectively 3 days af ... | 1993 | 8393061 |
| s-adenosylmethionine metabolism in herpes simplex virus type 2-infected cells. | infection of primary rat embryo cells with herpes simplex virus type 2 has previously been reported to produce a dramatic and rapid inhibition of cellular dna methylation. however, it has neither an immediate effect on s-adenosylmethionine breakdown nor on the relative pool sizes of s-adenosylmethionine and s-adenosylhomocysteine. | 1993 | 8393082 |
| synthesis and antiherpes virus activity of 1,5-anhydrohexitol nucleosides. | the synthesis of 1,5-anhydrohexitol nucleosides is described. these nucleoside analogues were obtained by alkylation of the heterocyclic bases with the tosylate 10 or by alkylation of the bases with the alcohol 12 under mitsunobu conditions. the compounds were evaluated for antiviral and cytostatic activity. highly selective activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) was noted for 1,5-anhydro-2,3-dideoxy-2-(5-iodouracil-1-yl)-d-arabino-hexitol 4b at a concentration o ... | 1993 | 8393114 |
| purification and characterization of the herpes simplex virus type 2-encoded uracil-dna glycosylase. | the herpes simplex virus type 2 (hsv-2) uracil-dna glycosylase (ung) was from the nuclei of infected kb cells using ion exchange, affinity, and chromatofocusing chromatography techniques. chromatography on dna cellulose revealed two distinct hsv-2-encoded ungs. one species, designated a, was purified approximately 324-fold, while the second species, designated b, was purified approximately 130-fold. the hsv ung species b was observed to unidirectionally convert to the a species, suggesting that ... | 1993 | 8393229 |
| transcriptional analysis of the herpes simplex virus type 1 region containing the trl/ul junction. | in this study we have used a combination of northern blot, cdna, and rnase protection analysis to characterize transcription occurring at the junction of the long terminal repeat (trl) and long unique segment (ul) of herpes simplex virus type 1 (hsv-1). two low abundance 5' colinear transcripts mapped between the junction of the trl/ul and the cap site of the primary latency associated transcripts (lat). analysis of the region containing the first four open translational reading frames (orfs) of ... | 1993 | 8395115 |
| inhibition of viral growth by an alpha-oligonucleotide directed to the splice junction of herpes simplex virus type-1 immediate-early pre-mrna species 22 and 47. | a 13-residue alpha-anomeric oligonucleotide [alpha-5'-d(gggcgtcctcctt)3'], 5'-substituted with a psoralen derivative, pso-alpha-13 psoralen linked to the 5' end of an alpha-anomeric n-residue oligonucleotide, was targeted to the acceptor splice junction of herpes simplex virus type-1 immediate-early pre-mrna species 22 and 47. inhibition of viral growth was observed upon irradiation of vero cells infected with herpes simplex virus type-1 and treatment with pso-alpha-13. the virus titer was decre ... | 1993 | 8396028 |
| the reliability of serological tests for the diagnosis of genital herpes: a critique. | the reliability and limitations of the currently used routine tests for herpes simplex virus type 2 (hsv-2) antibody in australia are reviewed. six case reports illustrate the clinical dangers of overinterpretation of the currently available kits and the need for a readily available specific hsv-2 antibody test. in sydney, hsv-2 causes approximately 85% of primary genital herpes and > 95% of recurrent genital herpes. due to the extensive serological cross-reactivity between hsv-1 and hsv-2, curr ... | 1993 | 8396232 |
| the rr1 gene of herpes simplex virus type 1 is uniquely trans activated by icp0 during infection. | as has been demonstrated for herpes simplex virus type 2, we show in this report that the herpes simplex virus type 1 ribonucleotide reductase large subunit (rr1) gene is trans activated in transient transfection assays by vp16 and icp0 but not by icp4. deletion analysis demonstrated that responsiveness to induction to vp16 resides in an octamer/taatgarat sequence of the rr1 promoter and that the tata box alone is sufficient to provide induction by icp0. the induction of the rr1 gene by icp0 but ... | 1993 | 8396674 |
| the effect of cytoplasmic domain mutations on membrane anchoring and glycoprotein processing of herpes simplex virus type 1 glycoprotein c. | the predicted amino acid sequence of herpes simplex virus type 1 glycoprotein c (hsv-1 gc) shows that it has the features of a typical type 1 integral membrane protein: a cleavable n-terminal signal sequence, a glycosylated ectodomain, a single transmembrane domain, and a small, charged cytoplasmic domain. in an earlier investigation of the function of the gc cytoplasmic domain, it was shown that the gc synthesized by a gc mutant, dl2, which lacked the last three residues of the transmembrane do ... | 1993 | 8396808 |
| nonoxynol-9 protects mice against vaginal transmission of genital herpes infections. | a vaginal application of a commercially available contraceptive jelly containing nonoxynol-9 (n9) prevented vaginal transmission of herpes simplex virus type 2 (hsv-2) infections in the mouse. when n9 jelly was delivered to the vagina with the virus inoculum, 20 s before inoculum, or 5 min before inoculum, mice were completely protected from visible infection (p < .001). protection lasted for at least 30 min (p < .03), and significant protection occurred even when the n9 jelly was delivered 15 m ... | 1993 | 8397261 |
| effect of indomethacin on ultraviolet radiation-induced recurrent herpes simplex virus disease in guinea-pigs. | exposure to u.v. radiation increases the local level of prostaglandins which may play a role in u.v. radiation-induced herpes simplex virus (hsv) recurrences. we used the guinea-pig model of u.v. radiation-induced recurrent genital hsv-2 disease for examining the effects of indomethacin, a prostaglandin inhibitor, on u.v.-induced recurrences. in the first experiment, performed 100 days after hsv-2 inoculation, treatment with indomethacin for 5 days begun 24 h before u.v.-irradiation decreased th ... | 1993 | 8397285 |
| [hevizos ointment efficacy in view of molecular pharmacology]. | the antiviral effect of 5-isopropyl-2'-deoxyuridine (ipdu) on herpes viruses (hsv-1 and hsv-2) is based on the sensitivity of virus dna to nucleases due to ipdu incorporation into the virus dna and the resulting change in dna conformation. virus selectivity is based on virus induced thymidine kinase activity and on hsv induction of polymerase enzymes catalyzing the incorporation. hevizos ointment containing ipdu has the following advantageous pharmacokinetic properties: high accumulation in the ... | 1993 | 8397468 |
| herpes simplex virus glycoprotein c: molecular mimicry of complement regulatory proteins by a viral protein. | herpes simplex virus (hsv) encodes a protein, glycoprotein c (gc), which binds to the third complement component, the central mediator of complement activation. in this study the structural and functional relationships of gc from hsv type 1 (hsv-1) and known human complement regulatory proteins factor h, properdin, factor b, complement receptor 1 (cr1) and 2 (cr2) were investigated. the interaction of gc with c3b was studied using purified complement components, synthetic peptides, antisera agai ... | 1993 | 8406590 |
| evaluation of a monoclonal antibody-based enzyme immunoassay for early detection of herpes simplex virus genital infection. | a monoclonal antibody-based enzyme immunoassay test for detection of herpes simplex virus (hsv) type-common antigen was evaluated in 40 women with vulvar lesion suspicious for genital hsv infection. the assay gave interpretable readings in 90% of the cases, with sensitivity and specificity of 100% and 92% respectively, a negative predictive value of 100%, and a positive predictive value of 75%. with a few limitations, the assay may be used for early diagnosis of genital hsv infection. | 1993 | 8407269 |
| an evaluation of oral ulcers in patients with aids and aids-related complex. | patients with hiv infection can have recurrent and persistent oral ulcers, not attributable to known infectious agents. | 1993 | 8408791 |
| autocrine secretion of interferon-alpha/beta and tumour necrosis factor-alpha synergistically activates mouse macrophages after infection with herpes simplex virus type 2. | resistance of mice to infection with herpes simplex virus type 2 (hsv-2) is strongly dependent on the function of macrophages (m phi). infection of mouse m phi with hsv-2 results in an early (4 to 10 h) activation of the cells with an enhanced respiratory burst generated after membrane triggering with a phorbol ester. the role of monokines produced during this infection was analysed. both interferon-alpha/beta (ifn-alpha/beta) and tumour necrosis factor-alpha (tnf-alpha) were produced within the ... | 1993 | 8409942 |
| [from herpes to zona]. | 1993 | 8410632 | |
| mollaret's meningitis associated with herpes simplex type 2 infection. | we describe three patients with benign recurrent aseptic meningitis (mollaret's meningitis). for one of these cases, the episodes of meningitis were associated with herpetic outbreaks. mollaret cells, which are a hallmark of mollaret's meningitis, were present in the csf from two of the three patients. in all cases, herpes simplex virus type 2 dna was present in the csf during the acute illness as detected by polymerase chain reaction amplification, although viral cultures from csf were all nega ... | 1993 | 8414021 |
| antiviral agents: characteristic activity spectrum depending on the molecular target with which they interact. | the target protein (enzyme) with which antiviral agents interact determines their antiviral activity spectrum. based on their activity spectrum, antiviral compounds could be divided into the following classes: (1) sulfated polysaccharides (i.e., dextran sulfate), which interact with the viral envelope glycoproteins and are inhibitory to a broad variety of enveloped viruses (i.e., retro-, herpes-, rhabdo-, and arenaviruses): (2) sah hydrolase inhibitors (i.e., neplanocin a derivatives), which are ... | 1993 | 8430518 |
| [clinical and pharmacologic review of antiviral agents]. | the author presents a review if pharmacokinetics, undesirable effects, drug interactions, dosage, indications and drug forms of antiviral chemotherapeutic agents used for systemic treatment: amantadine and rimantadine (influenza virus a), acyclovir (hsv-1, hsv-2 and vzv), ganciclovir (cmv), zidovudine (hiv), ribavirine (broad spectrum) and fosfocarnet (herpes viruses and hiv), mentioning also vidarabine. all mentioned preparations are at least in some countries commercially available. the author ... | 1993 | 8435845 |
| clinical reactivation of herpes simplex virus type 2 infection in seropositive pregnant women with no history of genital herpes. | to determine the risk for genital herpes and asymptomatic herpes simplex virus (hsv) shedding in late pregnancy and delivery in a population of hsv type 2 (hsv-2)-seropositive but previously asymptomatic pregnant women. | 1993 | 8439114 |
| serum antibodies and subsequent cervical neoplasms: a prospective study with 12 years of follow-up. | approximately 40,000 blood samples drawn in 1968-1972 by the social insurance institution's mobile clinic in finland have been stored. linking cancer incidence data obtained from the finnish cancer registry with this material yielded 32 cases of cervical cancer diagnosed after drawing of the blood sample. these 32 individuals and 64 matched controls were analyzed for serum antibodies indicative of past infection with chlamydia trachomatis, neisseria gonorrhoeae, cytomegalovirus, epstein-barr vir ... | 1993 | 8452120 |
| novel acyclonucleotides: synthesis and antiviral activity of alkenylphosphonic acid derivatives of purines and a pyrimidine. | a series of phosphonoalkenyl and (phosphonoalkenyl)oxy derivatives of purines and a pyrimidine were synthesized. these compounds are the first reported acyclonucleotides which incorporate the alpha,beta-unsaturated phosphonic acid moiety as the phosphate mimic and include compounds in which the acyclic substituent is attached to n-9 of a purine or n-1 of a pyrimidine by either a nitrogen-carbon or a nitrogen-oxygen bond. the phosphonoalkenyl-substituted compounds 7a-c, 8a-c, 9, 10, and 12 were p ... | 1993 | 8496903 |
| sero-prevalence of herpes simplex virus type-2 among cancer cervix patients. | serum samples were collected from 140 cancer cervix patients aged between 25-60 years and also from 20 age matched, married, healthy women to serve as controls. these sera were tested for hsv-2 antibodies by elisa test and hbsag by rpha test. hsv-2 antibodies were detected in 92 (65-71%) and hbsag in 25 (17.8%) cancer cervix patients. sera from control group were negative for hsv-2 antibodies and hbsag. | 1993 | 8206501 |
| underestimation of hsv-2 seroprevalence in a high-risk population by microneutralization assay. | complement independent neutralizing antibody assays (cina) have been used in seroepidemiologic studies and in diagnostic laboratories to distinguish between antibodies to herpes simplex virus types 1 (hsv-1) and 2 (hsv-2). the accuracy of cina has not been rigorously tested against protein-specific typing assays, such as western blot. | 1993 | 8211541 |
| identification of an infectious laryngotracheitis virus gene encoding an immunogenic protein with a predicted m(r) of 32 kilodaltons. | the nucleotide sequence of an infectious laryngotracheitis virus (iltv) gene which maps immediately upstream from the glycoprotein 60 (gp60) gene was determined. the gene, designated p32, encodes a predicted polypeptide of 298 amino acids with an estimated m(r) of 32,000 daltons. the predicted protein sequence has four potential n-glycosylation sites and a signal sequence at the n-terminal region. amino acid residues in the nh2-terminal region of the p32 protein exhibit similarity to glycoprotei ... | 1993 | 8212855 |
| the hsv-1 ul45 18 kda gene product is a true late protein and a component of the virion. | previously we constructed a null mutation in the hsv-1 ul45 gene, showed that the ul45 gene was not required for growth in vero cells, and confirmed that it coded for an 18 kda protein (r.j. visalli and c.r. brandt, virology 185:419-423, 1991). in this study, we have continued our characterization of the ul45 gene and the 18 kda protein. analysis of ul45 rna revealed that the gene was expressed late and was inhibited in the presence of phosphonoacetic acid (paa), indicating it is a gamma 2 class ... | 1993 | 8212858 |
| in vitro activity of a combretum micranthum extract against herpes simplex virus types 1 and 2. | the authors demonstrate in vitro antiviral activity of a methanolic extract of combretum micranthum leaves against hsv-1 and hsv-2. this activity is present only in the extract dissolved 7 days before the assay, but not in the freshly prepared extract, thus indicating the presence of inactive precursors which undergo spontaneous transformations into active compounds. the alkaline autooxidation of the methanolic extract promotes this rapid transformation. the precursors have been identified as co ... | 1993 | 8215303 |
| serum antibodies to herpes simplex virus types i and ii in depressed patients. | 1993 | 8218610 | |
| examination of cutaneous t-cell lymphoma for human herpesviruses by using the polymerase chain reaction. | the etiology of cutaneous t-cell lymphoma remains unknown, although an association with viral infection, in particular certain retroviruses and human herpesviruses, has been suggested. the purpose of this study was to examine skin biopsies of cutaneous t-cell lymphoma for the presence of epstein-barr virus, herpes simplex virus type 1 and type 2, and human herpesvirus-6 by using the polymerase chain reaction. lesional skin biopsies from 30 patients with cutaneous t-cell lymphoma were studied. co ... | 1993 | 8227605 |
| detection of herpes simplex virus in the cerebrospinal fluid of patients with encephalitis using the polymerase chain reaction. | herpes simplex encephalitis is a neurologic emergency demanding immediate institution of specific therapy in order to prevent mortality. diagnosis, however, is a complex matter with controversy existing over the appropriateness of brain biopsy. we report the demonstration of herpes simplex virus dna by means of the polymerase chain reaction (prc) in the cerebrospinal fluid of 3 patients with herpes simplex encephalitis. one of the patients suffered from brain-stem encephalitis with high intensit ... | 1993 | 8229072 |
| brief report: recurrent acyclovir-resistant genital herpes in an immunocompetent patient. | 1993 | 8232486 | |
| herpes simplex encephalitis. early diagnosis and immune activation in the acute stage and during long-term follow-up. | from a series of in all 93 patients with herpes simplex encephalitis (hse), verified by biopsy and/or the demonstration of intrathecal synthesis of antibodies to the virus, cerebrospinal fluid (csf) and serum samples were analysed and compared with samples from 80 patients with non-hse, i.e. acute encephalitis of non-hsv origin (approximately 50% with other known aetiology, 50% of unknown origin) treated on the suspicion of hse but in whom no signs of intrathecal hsv antibody synthesis were foun ... | 1993 | 8235453 |
| a solid-phase assay for the binding of peptidic subunit association inhibitors to the herpes simplex virus ribonucleotide reductase large subunit. | the herpes simplex virus (hsv) ribonucleotide reductase is comprised of two nonidentical homodimeric subunits whose association is essential for enzymatic activity. in order to evaluate the affinity of a series of peptidic inhibitors with the enzyme's large subunit (r1), we have developed a sensitive solid-phase binding assay. the assay entails the use of a nonneutralizing antibody directed against the r1 subunit of the enzyme to immobilize either the native holoenzyme from hsv-1-infected cells ... | 1993 | 8238915 |
| the epidemiology of herpes simplex types 1 and 2 infection of the genital tract in edinburgh 1978-1991. | the changing epidemiology of genital herpes in edinburgh is described in relation to herpes simplex virus (hsv) type 1 and herpes simplex virus type 2 infection over a period of 14 years. | 1993 | 8244358 |
| mapping the genetic region coding for herpes simplex virus resistance to mouse interferon alpha/beta. | herpes simplex virus (hsv) ocular virulence has been associated with strain sensitivity to mouse interferon (ifn)-alpha/beta. to identify the region of the virus genome associated with heightened resistance to this cytokine, intertypic recombinants were constructed using the intact genome of avirulent, ifn-sensitive hsv type 1 (strain 35) and xbai-digested dna from virulent, ifn-resistant hsv type 2 (strain 186). an intertypic recombinant, designated hsv-r4, was isolated which grew to titres 10- ... | 1993 | 8245849 |