Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
phylogeny of the viral hemorrhagic septicemia virus in european aquaculture.one of the most valuable aquaculture fish in europe is the rainbow trout, oncorhynchus mykiss, but the profitability of trout production is threatened by a highly lethal infectious disease, viral hemorrhagic septicemia (vhs), caused by the vhs virus (vhsv). for the past few decades, the subgenogroup ia of vhsv has been the main cause of vhs outbreaks in european freshwater-farmed rainbow trout. little is currently known, however, about the phylogenetic radiation of this ia lineage into subordina ...201627760205
in vivo virulence of viral haemorrhagic septicaemia virus (vhsv) in rainbow trout oncorhynchus mykiss correlates inversely with in vitro mx gene expression.the in vitro replication of viral haemorrhagic septicaemia virus (vhsv) isolates from each vhsv genotype and the associated cellular host mx gene expression were analysed. all the isolates were able to infect rtg-2 cells and induce increased mx gene expression (generic assay detecting isoforms 1 and 3 [mx1/3]). a trout pathogenic, genotype ia isolate (j167), showing high replication in rtg-2 cells (by infective titre and n gene expression) induced lower mx1/3 gene expression than observed in vhs ...201627066706
susceptibility of goldsinny wrasse, ctenolabrus rupestris l. (labridae), to viral haemorrhagic septicaemia virus (vhsv) genotype iii: experimental challenge and pathology.cleaner fish, such as wrasse, are being increasingly used to combat the sea lice infestation of atlantic salmon (salmo salar l.) in many european countries. to determine susceptibility of the goldsinny wrasse (ctenolabrus rupestris l.) and pathogenesis of the viral haemorrhagic septicaemia virus (vhsv) genotype iii isolate 12-654, previously associated with vhsv infection in the shetland islands in 2012, fish were experimentally challenged by intraperitoneal injection (ip), bath immersion and co ...201627016771
first in-depth analysis of the novel th2-type cytokines in salmonid fish reveals distinct patterns of expression and modulation but overlapping bioactivities.il-4 and il-13 are closely related canonical type-2 cytokines in mammals and have overlapping bioactivities via shared receptors. they are frequently activated together as part of the same immune response and are the signature cytokines produced by t-helper (th)2 cells and type-2 innate lymphoid cells (ilc2), mediating immunity against extracellular pathogens. little is known about the origin of type-2 responses, and whether they were an essential component of the early adaptive immune system th ...201626870894
distribution of t cells in rainbow trout (oncorhynchus mykiss) skin and responsiveness to viral infection.although the skin constitutes the first line of defense against waterborne pathogens, there is a great lack of information regarding the skin associated lymphoid tissue (salt) and whether immune components of the skin are homogeneously distributed through the surface of the fish is still unknown. in the current work, we have analyzed the transcription of several immune genes throughout different rainbow trout (oncorhynchus mykiss) skin areas. we found that immunoglobulin and chemokine gene trans ...201626808410
isolation and identification of viral hemorrhagic septicemia virus (vhsv) from farmed rainbow trout (oncorhynchus mykiss) in iran.viral hemorrhagic septicemia virus (vhsv) is a rhabdovirus that causes one of the most important fish diseases in rainbow trout (oncorhynchus mykiss) production industry. during the present study from october 2014 to july 2015, the virus causing viral hemorrhagic septicemia (vhs) was isolated and identified in rainbow trout farms from five of sixteen farms experiencing mass mortalities in six provinces of iran with major trout production. cumulative mortalities at vhsv-positive farms ranged from ...201626777311
sublethal concentrations of ichthyotoxic alga prymnesium parvum affect rainbow trout susceptibility to viral haemorrhagic septicaemia virus.ichthyotoxic algal blooms are normally considered a threat to maricultured fish only when blooms reach lethal cell concentrations. the degree to which sublethal algal concentrations challenge the health of the fish during blooms is practically unknown. in this study, we analysed whether sublethal concentrations of the ichthyotoxic alga prymnesium parvum affect the susceptibility of rainbow trout oncorhynchus mykiss to viral haemorrhagic septicaemia virus (vhsv). during exposure to sublethal alga ...201626758652
identification and expression modulation of a c-type lectin domain family 4 homologue that is highly expressed in monocytes/macrophages in rainbow trout (oncorhynchus mykiss).the c-type lectin domain containing (clec) receptors including cd209 are expressed in vivo by monocytes, monocyte-derived macrophages and dendritic cells and by in vitro generated monocyte-derived cells. this paper reports the cloning and sequencing of a lectin molecule, clec4t1, in rainbow trout that is a homologue of the clec4 family. the expression pattern of the clec4t1 was investigated in vivo after infection with a bacterial pathogen and in cultured macrophages after modulation with microb ...201626279216
hemato - immunological and biochemical parameters, skin antibacterial activity, and survival in rainbow trout (oncorhynchus mykiss) following the diet supplemented with mentha piperita against yersinia ruckeri.this study was aimed to assess the potential effects of mentha piperita on the hemato - immunological and biochemical parameters, skin antibacterial activity and protection against yersinia ruckeri infection in rainbow trout oncorhynchus mykiss. fish were divided into 4 groups before being fed diets supplemented with 0, 1, 2 and 3% of mentha piperita (mp) plant extract for 8 weeks. dose-dependent increases immune (both in skin mucus and blood serum) and hematological parameters (number of red an ...201627245867
yersinia ruckeri lipopolysaccharide is necessary and sufficient for eliciting a protective immune response in rainbow trout (oncorhynchus mykiss, walbaum).enteric redmouth disease (erm), caused by yersinia ruckeri, has been controlled successfully using immersion-applied bacterin vaccines for several decades. while the host response to vaccination and the mechanism of protection of this vaccine have been elucidated, the bacterial components eliciting protection have remained unclear. here we show that highly purified serotype o1 y. ruckeri lipopolysaccharide (lps) is sufficient to induce a protective response to experimental challenge in rainbow t ...201626766181
re-examination of the rainbow trout (oncorhynchus mykiss) immune response to flagellin: yersinia ruckeri flagellin is a potent activator of acute phase proteins, anti-microbial peptides and pro-inflammatory cytokines in vitro.flagellin is the principal component of bacterial flagellum and a major target of the host immune system. to provide new insights into the role of flagellin in fish immune responses to flagellated microorganisms, a recombinant flagellin from yersinia ruckeri (ryrf) was produced and its bioactivity investigated in the trout macrophage cell line rts-11 and head kidney cells. ryrf is a potent activator of pro-inflammatory cytokines, acute phase proteins, antimicrobial peptides and subunits of the i ...201626719024
genome sequence of the fish pathogen yersinia ruckeri strain 150, isolated from diseased rainbow trout.we present here the draft genome of a pathogenic yersinia ruckeri strain, isolated from rainbow trout (oncorhynchus mykiss) affected by enteric redmouth disease. the chromosome has 3,826,775 bp, a gc content of 46.88%, and is predicted to contain 3,538 coding sequences. the data will be useful for comparative pathogenicity studies.201627908991
characterisation of zbtb46 and dc-script/znf366 in rainbow trout, transcription factors potentially involved in dendritic cell maturation and activation in fish.zbtb46 and dc-script/znf366 are two zinc finger transcription factors that play important roles in regulating differentiation of dendritic cells in mammals. in this study, the zbtb46 and dc-script/znf366 homologues were identified in rainbow trout oncorhynchus mykiss and their expression analysed in vivo and in vitro. as transcription factors, they are well conserved in sequence, genomic organisation and gene synteny. their expression was differentially modulated by bacterial and viral pamps in ...201627889532
effect of feed supplementation with kynurenic acid on the morphology of the liver, kidney and gills in rainbow trout (oncorhynchus mykiss walbaum, 1792), healthy and experimentally infected with yersinia ruckeri.kynurenic acid (kyna) is an endogenous substance produced on the kynurenine pathway which is primarily known for its neuroactive properties. recently, it has been proven that kyna is a selective ligand for g protein-coupled receptor (gpr 35), presented on immunocompetent cells such as t lymphocytes. this opens up new possibilities of its application as an immunostimulating substance in aquaculture. thus far, no histopathological investigations in fish have been completed to evaluate influence of ...201627690267
yersinia ruckeri isolates recovered from diseased atlantic salmon (salmo salar) in scotland are more diverse than those from rainbow trout (oncorhynchus mykiss) and represent distinct subpopulations.yersinia ruckeri is the etiological agent of enteric redmouth (erm) disease of farmed salmonids. enteric redmouth disease is traditionally associated with rainbow trout (oncorhynchus mykiss, walbaum), but its incidence in atlantic salmon (salmo salar) is increasing. yersinia ruckeri isolates recovered from diseased atlantic salmon have been poorly characterized, and very little is known about the relationship of the isolates associated with these two species. phenotypic approaches were used to c ...201627451448
antimicrobial peptide cap18 and its effect on yersinia ruckeri infections in rainbow trout oncorhynchus mykiss (walbaum): comparing administration by injection and oral routes.the antimicrobial peptide cap18 has been demonstrated to have a strong in vitro bactericidal effect on yersinia ruckeri, but its activity in vivo has not been described. in this work, we investigated whether cap18 protects rainbow trout oncorhynchus mykiss (walbaum) against enteric red mouth disease caused by this pathogen either following i.p. injection or by oral administration (in feed). it was found that injection of cap18 into juvenile rainbow trout before exposure to y. ruckeri was associa ...201727334068
the flagellar master operon flhdc is a pleiotropic regulator involved in motility and virulence of the fish pathogen yersinia ruckeri.to investigate the function of the master flagellar operon flhdc in the fish pathogen yersinia ruckeri and compare the effect of a constructed flhd mutation to a naturally occurring flir mutation causing loss-of-motility in emergent biotype 2 (bt2) strains.201727981729
effects of dietary yeast inclusion and acute stress on post-prandial whole blood profiles of dorsal aorta-cannulated rainbow trout.yeast is a potential alternative to fish meal in diets for farmed fish, yet replacing more than 50 % of fish meal results in reduced fish growth. in a 4-week experiment, 15 rainbow trout (oncorhynchus mykiss) were cannulated and fed three diets each week: 30 % fish meal as a control (fm); 60 % replacement of fish meal protein, on a digestible basis, with saccharomyces cerevisiae (sc); and 60 % replacement with wickerhamomyces anomalus and s. cerevisiae mix (wa). blood was collected at 0, 3, 6, 1 ...201727677483
microbiome dynamic modulation through functional diets based on pre- and probiotics (mannan-oligosaccharides and saccharomyces cerevisiae) in juvenile rainbow trout (oncorhynchus mykiss).this study used high-throughput sequencing to evaluate the intestinal microbiome dynamics in rainbow trout (oncorhynchus mykiss) fed commercial diets supplemented with either pre- or probiotics (0·6% mannan-oligosaccharides and 0·5% saccharomyces cerevisiae respectively) or the mixture of both.201728256031
lhx8 interacts with a novel germ cell-specific nuclear factor containing an nbl1 domain in rainbow trout (oncorhynchus mykiss).lhx8 is an important transcription factor that is preferentially expressed in germ cells. lhx8 null mice are infertile due to lack of oocytes and impairment of the transition from primordial follicles to primary follicles. lhx8 deficiency also affects the expression of many important oocyte-specific genes. in this study, we report the characterization of rainbow trout lhx8 genes and identification of a novel germ cell-specific nuclear factor that interacts with lhx8. two lhx8 genes, lhx8a and lh ...201728151980
analytical validation of a reverse transcriptase droplet digital pcr (rt-ddpcr) for quantitative detection of infectious hematopoietic necrosis virus.infectious hematopoietic necrosis virus (ihnv) is an important pathogen of salmonid fishes. a validated universal reverse transcriptase quantitative pcr (rt-qpcr) assay that can quantify levels of ihnv in fish tissues has been previously reported. in the present study, we adapted the published set of ihnv primers and probe for use in a reverse-transcriptase droplet digital pcr (rt-ddpcr) assay for quantification of the virus in fish tissue samples. the rt-ddpcr and rt-qpcr assays detected 13 phy ...201728347708
comparison of selected nonlethal samples from adult steelhead for detection of infectious hematopoietic necrosis virus using cell culture.nonlethal sampling techniques have previously been evaluated for detection of a variety of viral salmonid pathogens. however, many of these studies have used molecular assays in lieu of widely accepted cell culture techniques to evaluate the sampled tissues. samples were collected from female steelhead oncorhynchus mykiss broodstock using three potential nonlethal sampling methods (mucus/skin scrape, pectoral fin clip, and gill tissue biopsy) and evaluated for the presence of infectious hematopo ...201728324676
preliminary study of an oral vaccine against infectious hematopoietic necrosis virus using improved yeast surface display technology.infectious hematopoietic necrosis virus (ihnv) is a common pathogen that causes severe disease in the salmonid aquaculture industry. because oral vaccines induce more efficient mucosal immunity than parenteral immunization, an oral vaccine was developed with an improved yeast cell surface display technology to induce an immune response to ihnv. the oral yeast vaccine, designated eby100/pyd1-bi-g, was delivered orally to rainbow trout (oncorhynchus mykiss) on days 1 and 32, and the nonspecific an ...201728285182
dna vaccination partially protects muskellunge against viral hemorrhagic septicemia virus (vhsv-ivb).a dna vaccine containing the glycoprotein (g) gene of the north american viral hemorrhagic septicemia virus (vhsv) genotype ivb was developed to evaluate the immune response of fish following vaccination and evaluate its efficacy in protecting a susceptible species, the muskellunge esox masquinongy, against vhsv-ivb challenge. seven weeks (539 degree-days) following vaccination with 10 μg of either pvhsivb-g or a control plasmid, muskellunge were challenged by immersion with 10(5) plaque-forming ...201728225652
replication and shedding kinetics of infectious hematopoietic necrosis virus in juvenile rainbow trout.viral replication and shedding are key components of transmission and fitness, the kinetics of which are heavily dependent on virus, host, and environmental factors. to date, no studies have quantified the shedding kinetics of infectious hematopoietic necrosis virus (ihnv) in rainbow trout (oncorhynchus mykiss), or how they are associated with replication, making it difficult to ascertain the transmission dynamics of this pathogen of high agricultural and conservation importance. here, the repli ...201727771253
outbreak of infectious pancreatic necrosis virus (ipnv) in farmed rainbow trout in china.infectious pancreatic necrosis virus (ipnv) is a member of the aquabirnavirus genus, which caused mass mortality (nearly 100%) in farmed rainbow trout (oncorhynchus mykiss) in aquaculture farms in 2016, china. major clinical signs included decreased appetite, mucous-like stools, and darkened pigmentation. pathological changes in moribund fish were observed, such as marked vacuolar degeneration of the pancreatic cells with pyknotic nucleus and decreased zymogen granules, severe hemorrhage in the ...201728232067
complete genomic sequence of an infectious pancreatic necrosis virus isolated from rainbow trout (oncorhynchus mykiss) in china.infectious pancreatic necrosis (ipn) is a significant disease of farmed salmonids resulting in direct economic losses due to high mortality in china. however, no gene sequence of any chinese infectious pancreatic necrosis virus (ipnv) isolates was available. in the study, moribund rainbow trout fry samples were collected during an outbreak of ipn in yunnan province of southwest china in 2013. an ipnv was isolated and tentatively named chrtm213. we determined the full genome sequence of the ipnv ...201727858313
gyrodactylus salmonis infection impairs the olfactory system of rainbow trout.monogenean worms are ectoparasites that are known to be infectious to a wide variety of fish. few species of monogenean parasites have been reported in the olfactory chamber of fish in current peer-reviewed literature. however, the impacts of these parasites on the olfactory system are not well understood. in this study, the effects of gyrodactylus salmonis on the olfactory system structure and performance were investigated in rainbow trout (oncorhynchus mykiss). the olfactory performance of the ...201728105714
comparative analyses within gyrodactylus (platyhelminthes: monogenea) mitochondrial genomes and conserved polymerase chain reaction primers for gyrodactylid mitochondrial dna.in this study, we describe the complete mitochondrial genomes of gyrodactylus brachymystacis and gyrodactylus parvae infecting rainbow trout (oncorhynchus mykiss) and the invasive topmouth gudgeon (pseudorasbora parva), respectively. the two circular genomes have a common genome organization found in other gyrodactylus species. comparative analyses of mitochondrial genomes from six gyrodactylus species were carried out to determine base composition, codon usage, transfer rna and ribosomal rna ge ...201727502106
molecular characterization of a novel orthomyxovirus from rainbow and steelhead trout (oncorhynchus mykiss).a novel virus, rainbow trout orthomyxovirus (rbtov), was isolated in 1997 and again in 2000 from commercially-reared rainbow trout (oncorhynchus mykiss) in idaho, usa. the virus grew optimally in the chse-214 cell line at 15°c producing a diffuse cytopathic effect; however, juvenile rainbow trout exposed to cell culture-grown virus showed no mortality or gross pathology. electron microscopy of preparations from infected cell cultures revealed the presence of typical orthomyxovirus particles. the ...201728088362
characterisation of arginase paralogues in salmonids and their modulation by immune stimulation/ infection.in this study we show that four arginase isoforms (arg1a, arg1b, arg2a, arg2b) exist in rainbow trout (oncorhynchus mykiss) and atlantic salmon (salmo salar). we have characterised these molecules in terms of a) sequence analysis, b) constitutive expression in different tissues, and modulated expression following c) stimulation of head kidney macrophages in vitro, or d) vaccination/infection with yersinia ruckeri and e) parasite infection (agd caused by paramoeba perurans and pkd caused by tetra ...201728025160
ecological risk assessment of pharmaceuticals in the receiving environment of pharmaceutical wastewater in pakistan.the pharmaceutical industry of pakistan is growing with an annual growth rate of 10%. besides this growth, this industry is not complying with environmental standards, and discharging its effluent into domestic wastewater network. only limited information is available about the occurrence of pharmaceutical compounds (pcs) in the environmental matrices of pakistan that has motivated us to aim at the occurrence and ecological risk assessment of 11 pcs of different therapeutic classes in the wastew ...201727810578
different phenotypes of mature biofilm in flavobacterium psychrophilum share a potential for virulence that differs from planktonic state.flavobacterium psychrophilum is the etiological agent of bacterial coldwater disease and the rainbow trout fry syndrome in salmonid aquaculture worldwide. however, there have been few studies into the capacity of f. psychrophilum to form biofilms and how these cellular accretions differ from planktonic cells or how they affect potential virulence. we evaluated the biofilm formation by three chilean isolates of f. psychrophilum (lm-02-fp, lm-06-fp, and lm-13-fp) and two non-chilean strains (jip02 ...201728361040
genetic and serological diversity of flavobacterium psychrophilum isolates from salmonids in united kingdom.flavobacterium psychrophilum is one of the most important bacterial pathogens affecting cultured rainbow trout (oncorhynchus mykiss) and is increasingly causing problems in atlantic salmon (salmo salar l.) hatcheries. little is known about the heterogeneity of f. psychrophilum isolates on uk salmonid farms. a total of 315 f. psychrophilum isolates, 293 of which were collected from 27 sites within the uk, were characterised using four genotyping methods and a serotyping scheme. a high strain dive ...201728284613
dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (oncorhynchus mykiss) measured using a novel gexp multiplex, rt-pcr assay.a gexp multiplex, rt-pcr assay was developed and optimized that simultaneously measures expression of a suite of immune-relevant genes in rainbow trout (oncorhynchus mykiss), concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. the dataset includes expression values for drpt, il11a, il1b1, il1b2, il1b3, il1r-like-1(e3-5), il1r-like-1(e9-11), il1r1-like-a, il1r1-like-b, il1r2, saa, tnfa1, tnfa2, tnfa3, tnfrsf1a, tnfrsf1a-like-a, tnfrsf1a ...201728239633
in vivo adherence of flavobacterium psychrophilum to mucosal external surfaces of rainbow trout (oncorhynchus mykiss) fry.the adherence of flavobacterium psychrophilum to surfaces of epithelial tissues has been inconclusively suggested as a mechanism, which enables the bacterium to invade the host. hence, the present study aimed to examine the adherence of the cells of two colony phenotypes, smooth and rough, of f. psychrophilum to mucosal tissues of rainbow trout fry and to test the skin mucus as a nutrient for the growth of f. psychrophilum. fish were immersed in water containing 10(6 ) cfu( ) ml(-1) f. psychroph ...201728188658
expansion and differentiation of igm(+) b cells in the rainbow trout peritoneal cavity in response to different antigens.to date, intraperitoneal (i.p.) injection seems to be the most effective vaccination route in aquaculture, as many i.p. administered fish vaccines are capable of conferring strong and long-lasting immune responses. despite this, how peritoneal leukocytes are regulated upon antigen encounter has only been scarcely studied in fish. although, in the past, myeloid cells were thought to be the main responders to peritoneal inflammation, a recent study revealed that igm(+) b cells are one of the main ...201728088353
rainbow trout (oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens.commensal bacteria co-exist on the mucosal surfaces of all vertebrates. the host's mucosal immune system must tolerate commensals while fighting pathogens. one of the mechanisms used by the mucosal immune system to maintain homeostasis is the secretion of immunoglobulins (igs) across epithelial barriers, which is achieved via the polymeric immunoglobulin receptor (pigr). rainbow trout pigr is known to transport igt and igm across epithelia. however, other biological functions for trout pigr or t ...201728150752
molecular characterization of infectious pancreatic necrosis virus strains isolated from the three types of salmonids farmed in chile.the infectious pancreatic necrosis virus (ipnv) causes significant economic losses in chilean salmon farming. for effective sanitary management, the ipnv strains present in chile need to be fully studied, characterized, and constantly updated at the molecular level.201728143585
application of passive sampling to characterise the fish exometabolome.the endogenous metabolites excreted by organisms into their surrounding environment, termed the exometabolome, are important for many processes including chemical communication. in fish biology, such metabolites are also known to be informative markers of physiological status. while metabolomics is increasingly used to investigate the endogenous biochemistry of organisms, no non-targeted studies of the metabolic complexity of fish exometabolomes have been reported to date. in environmental chemi ...201728216558
environmental indicators in effluent assessment of rainbow trout (oncorhynchus mykiss) reared in raceway system through phosphorus and nitrogen.the phosphorus and nitrogen discharge via effluent of intensive trout farming system was quantified through the use of environmental indicators. the nutrient loads, the mass balance, the estimated amount of nutrients in feed and the amount of nutrients converted in fish biomass were calculated based on the concentrations of phosphorus (p) and nitrogen (n) in the feed and in the water. of the offered feed, 24.75 kg were available as p and 99.00 kg as n, of these, 9.32 kg p (38%) and 29.12 kg n (2 ...201727224731
pathogenicity of mexican isolates of aeromonas sp. in immersion experimentally-infected rainbow trout (oncorhynchus mykiss, walbaum 1792).ten species of aeromonas have been previously identified in farmed rainbow trout from mexico. the aim of the current study was to investigate the pathogenicity of 10 aeromonas isolates belonging to 10 different aeromonas species in immersion experimentally-infected rainbow trout fry. isolates of a. bestiarum, a. hydrophila, a. salmonicida, and a. veronii produced significant mortality (8.8%, 12.2%, 18.8%, and 8.8%, respectively). isolates of a. caviae and a. sobria produced no significantly mort ...201728209550
consecutive emamectin benzoate and deltamethrin treatments affect the expressions and activities of detoxification enzymes in the rainbow trout (oncorhynchus mykiss).rainbow trout (oncorhynchus mykiss) subjected to three consecutive, alternating treatments with emamectin benzoate (emb) and deltamethrin (dm) during outbreaks of caligus rogercresseyi in a farm located in southern chile (hornopiren, chiloé), were studied to determine the effects of these treatments on the protein and enzymatic activity levels of cytochrome p450 1a (cyp1a), flavin-containing monooxygenase (fmo) and glutathione s-transferase (gst) in different tissues. consecutive and alternating ...201727765649
invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout.the literature on cell lines that have been developed from rainbow trout (rt) (oncorhynchus mykiss) is reviewed to illustrate three new terms: invitromatics, invitrome, and invitroomics. invitromatics is defined as the history, development, characterization, engineering, storage, and sharing of cell lines. rt invitromatics differs from invitromatics for humans and other mammals in several ways. nearly all the rt cell lines have developed through spontaneous immortalization. no rt cell line under ...201728374170
hypothermic storage of isolated spermatogonia and oogonia from rainbow trout (oncorhynchus mykiss).a growing number of fish species are endangered due to human activities. a short- or long-time preservation of gametes could conserve genetic resources of threatened fish species. the aim of this study was to evaluate a hypothermic condition for short-term preservation of spermatogonia and oogonia cells isolated from immature transgenic rainbow trout, oncorhynchus mykiss, and to determine the maximum time point for further transplantation. viability rate of germ cells was investigated after isol ...201728302378
towards the development of a sustainable soya bean-based feedstock for aquaculture.soya bean (glycine max (l.) merr.) is sought after for both its oil and protein components. genetic approaches to add value to either component are ongoing efforts in soya bean breeding and molecular biology programmes. the former is the primary vegetable oil consumed in the world. hence, its primary usage is in direct human consumption. as a means to increase its utility in feed applications, thereby expanding the market of soya bean coproducts, we investigated the simultaneous displacement of ...201727496594
antimicrobial effect of the biotronic® top3 supplement and efficacy in protecting rainbow trout (oncorhynchus mykiss) from infection by aeromonas salmonicida subsp. salmonicida.demand for more environmentally friendly practices have led to the adoption of several feed supplements by the fish farming industry. in the present study, we investigated a commercially available formula that includes a mixture of three compounds: organic acids, a phytochemical and biomin® permeabilizing complex. this mixture demonstrated antimicrobial properties in vitro and was able to inhibit growth of multiple species of aquatic bacterial pathogens, including aeromonas salmonicida. bacteria ...201728343146
effect of dietary β-glucan on growth, survival and regulation of immune processes in rainbow trout (oncorhynchus mykiss) infected by aeromonas salmonicida.the present study evaluated the effects of dietary β-glucan (0, 0.05%, 0.1%, and 0.2%) on growth performance after 42 days of feeding. thereafter, rainbow trout (oncorhynchus mykiss) were infected with aeromonas salmonicida, and survival rates as well as the regulating processes of stress- and immune-related factors were analyzed. in general, higher dietary β-glucan levels obviously improved specific growth rate (sgr), weight gain (wg) and feed efficiency (fe) (p ≤ 0.05). survival rates in β-glu ...201728286256
subunit vaccine candidates against aeromonas salmonicida in rainbow trout oncorhynchus mykiss.aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis and a major fish health problem in salmonid aquaculture worldwide. injection vaccination with commercial mineral oil-adjuvanted bacterin vaccines has been partly successful in preventing the disease but in danish rainbow trout (oncorhynchus mykiss, walbaum) aquaculture furunculosis outbreaks still occur. in this study we tested the efficacy of experimental subunit vaccines against a. salmonicida infection in rainbo ...201728182704
characterization and mechanism of anti-aeromonas salmonicida activity of a marine probiotic strain, bacillus velezensis v4.the bacterium aeromonas salmonicida is the causative agent of furunculosis, a systemic, ubiquitous disease of fish in the salmon family, characterized by high mortality and morbidity. probiotics are a promising approach for prevention of furunculosis in aquaculture. a bacterial strain with anti-a. salmonicida properties, bacillus velezensis v4, was isolated and the mechanisms underlying these properties were investigated. anti-a. salmonicida compounds present in cell-free supernatant of v4 were ...201728074223
aeromonas salmonicida type iii secretion system-effectors-mediated immune suppression in rainbow trout (oncorhynchus mykiss).aeromonas salmonicida subsp. salmonicida, the etiologic agent of furunculosis, is a major pathogen in aquaculture. together with other pathogens, it is characterized by the presence of a type 3 secretion system (t3ss). the t3ss is the main virulence mechanism of a. salmonicida. it is used by the bacterium to secrete and translocate several toxins and effector proteins into the host cell. some of these factors have a detrimental impact on the integrity of the cell cytoskeleton, likely contributin ...201727923746
multiple gene and transcript variants encoding trout c-polysaccharide binding proteins are differentially but strongly induced after infection with aeromonas salmonicida.two 'trout c-polysaccharide-binding proteins,' tcbp1 and -2, with relevance to early inflammatory events have been discovered in the last century. the present study characterises the respective cdna sequences from rainbow trout (oncorhynchus mykiss), including multiple tcbp1 transcript variants. these variants are generated either by the use of alternative splice sites or the exclusion of exons. the longest mrna isoform, tcbp1-1, encodes a 245-aa protein with a large signal peptide and a complem ...201727836722
st2 from rainbow trout quenches tlr signalling, localises at the nuclear membrane and allows the nuclear translocation of myd88.the mammalian interleukin 1 receptor-like 1 receptor (il1rl1), commonly known as st2, is thought to downregulate tlr signalling by sequestering the signalling adapter myd88 (myeloid differentiation primary response protein 88). st2 sequences are known in several fish species, but none of them have functionally been examined. we characterised st2 from rainbow trout (oncorhynchus mykiss) and the structure of its encoding gene. the primary sequence of st2 is only weakly conserved from fish to human ...201727776995
positive correlation between aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout oncorhynchus mykiss evaluated by a tail fin infection model.rainbow trout, oncorhynchus mykiss (walbaum), are able to raise a protective immune response against aeromonas salmonicida subsp. salmonicida (as) following injection vaccination with commercial vaccines containing formalin-killed bacteria, but the protection is often suboptimal under danish mariculture conditions. we elucidated whether protection can be improved by increasing the concentration of antigen (formalin-killed bacteria) in the vaccine. rainbow trout juveniles were vaccinated by intra ...201727593619
dietary myrtle (myrtus communis l.) improved non-specific immune parameters and bactericidal activity of skin mucus in rainbow trout (oncorhynchus mykiss) fingerlings.the present study examined the effects of dietary myrtle (myrtus communis l.) on non-specific immune parameters and bactericidal activity of skin mucus in rainbow trout (oncorhynchus mykiss) fingerlings. three hundred and sixty fingerlings (6.50 ± 0.55 g (were distributed in twelve cages (65 × 65 × 65 cm) with a metal framework. the study included four treatments repeated in triplicates. the treatments were feeding trouts with experimental diets containing different levels (0, 0.5, 1 and 1.5%) o ...201728330807
dietary β-glucans differentially modulate immune and stress-related gene expression in lymphoid organs from healthy and aeromonas hydrophila-infected rainbow trout (oncorhynchus mykiss).although β-glucans stimulating effects have already been demonstrated on the immune system of numerous animal species, available data remain relatively variable and more research should be done regarding the complexity of underlying mechanisms. in this context, the present study aimed to evaluate the stress and immune-related effects of dietary β-glucans (i.e. macrogard(®)) by considering a number of influencing factors such as the dose (0, 0.1, 0.2 and 0.5% in food), feeding duration (15 versus ...201728232282
effect of ammonia-n and pathogen challenge on complement component 8α and 8β expression in the darkbarbel catfish pelteobagrus vachellii.the complement components c8α and c8β mediate the formation of the membrane attack complex (mac) to resist pathogenic bacteria and play important roles in innate immunity. full-length complement c8α (pv-c8α) and c8β (pv-c8β) cdna were identified in the darkbarbel catfish pelteobagrus vachellii, and their mrna expression levels were analyzed after ammonia-n and pathogen treatment. the pv-c8α gene contained 1983 bp, including a 1794-bp open reading frame (orf) encoding 598 amino acids. the pv-c8β ...201728027983
Refresh
firstprev
...11121314151617181920
nextlast
Displaying items 1901 - 1957 of 1957