Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| quantification of igg and igg4 antibodies to bee venom phospholipase a2 by competitive inhibition in elisa. | phospholipase a2 (pla) is the major antigen of bee venom. individuals often stung by bees, such as bee keepers, show a restricted immune response mainly of anti-pla igg4 antibodies. in contrast, patients allergic to bee venom produce high levels of pla-specific ige. this isotype restriction, the clinical relevance and the well defined structure of the pla antigen, provide a useful model for the study of the principles regulating isotype expression in the human antibody response. a fundamental re ... | 1989 | 2708823 |
| toxicity of colocynithin and hydrated colocynithin from alcoholic extract of citrullus colocynthis pulp. | the comparative toxicity of the alcoholic extract of citrullus colocynthis pulp; colocynithin and hydrated colocynithin have been isolated and tried against seven insect species. the american cockroaches were more susceptible for both compounds than the german one, adult honey bee were more affected with colocynithin than adult housefly. while hydrated colocynithin was more toxic to housefly than honey bee, cotton leafworm was less affected with both toxicants. bed bug was less affected with col ... | 1989 | 2708854 |
| comparative studies on the protein composition of hymenopteran venom reservoirs. | the proteins of venom reservoirs from 25 hymenopteran species from 21 genera were investigated with regard to their protein composition and immunological similarities. it was found that low mol. wt proteins and polypeptides are typical for the venoms of ants, social wasps and bees. six species of ichneumonoid parasitic wasps lack these low mol. wt proteins. except for bee venoms none of the low mol. wt proteins contain mannose. the venoms of ichneumonoid parasites and ants contained primarily ac ... | 1989 | 2711410 |
| factors influencing the hemolysis of human erythrocytes by cardiotoxins from naja naja kaouthia and naja naja atra venoms and a phospholipase a2 with cardiotoxin-like activities from bungarus fasciatus venom. | the effects of red blood cell age and incubation conditions (temperature, divalent cation type and concentration, ph and glucose) on hemolysis induced by cardiotoxin fractions from naja naja atra and naja naja kaouthia venoms, a phospholipase a2 with cardiotoxin-like activities from bungarus fasciatus venom and bee venom phospholipase a2 were examined. hemolysis by the snake venom toxins was dependent on red blood cell age (aged more susceptible than fresh) and the temperature of incubation (37 ... | 1989 | 2718193 |
| stimulation of enzymatic activity in filament preparations of casein kinase ii by polylysine, melittin, and spermine. | casein kinase ii (ckii) has been purified from bovine heart tissue. under conditions of low salt (0.05 m nacl, 10 mm mgcl2), ckii forms structured aggregates that appear as filaments similar to results obtained with drosophila ckii [c.v.c. glover (1986) j. biol. chem. 261:14349]. the aggregates have been analyzed by sucrose density gradients and electron microscopy. filament preparations of the enzyme have reduced but measurable kinase activity. the addition of salt restores activity. various mo ... | 1989 | 2725485 |
| soluble copolymers of yellow jacket, yellow hornet and white faced hornet with human albumin for venom immunotherapy. | soluble polymers have been prepared by polymerizing human serum albumin (hsa) with either yellow jacket venom (yjv), yellow hornet venom (yhv) or white faced hornet venom (wfhv). the venom-albumin polymer preparations were fractionated on sephacryl s-300 to have a molecular weight range higher than catalase (2.4 x 10(5) daltons). rabbits were immunized with either yellow jacket venom-albumin polymer (yjvap), yellow hornet venom-albumin polymer (yhvap) or white faced hornet venom-albumin polymer ... | 1989 | 2737470 |
| the gravimetric method for the determination of residual moisture in freeze-dried biological products. | the gravimetric test for the determination of residual moisture in freeze-dried biological products performed in a humidity- and temperature-controlled room with the use of scrupulous gravimetric analytical technique can be used to accurately determine residual moisture in freeze-dried biological products such as antihemophilic factor (human) or honey bee venom allergenic extract. this method determines the first water of hydration of sodium tartrate dihydrate (7.93%) to within 1.3% of the calcu ... | 1989 | 2743789 |
| action of a thionin isolated from nuts of pyrularia pubera on human erythrocytes. | pyrularia thionin is a strongly basic peptide of 47 amino acids isolated from pyrularia pubera. this peptide, a member of the thionin family, is hemolytic, cytotoxic and neurotoxic. the characteristics of the hemolytic activity on human erythrocytes are as follows: (1) the peptide does not itself have any phospholipase activity in a micellar assay system with egg yolk phosphatidylcholine, as evidenced by a lack of ph change or uptake of oxygen in the presence of lipoxidase; (2) erythrocyte membr ... | 1989 | 2749750 |
| effect of oxidative stress on membrane phospholipid and protein organization in human erythrocytes. | membrane phospholipid and protein organization was studied in intact human erythrocytes exposed to phenylhydrazine, an oxidative agent inducer. the evaluation of the membrane phospholipid and protein organization was carried out in terms of asymmetric distribution across the membrane bilayer for the phospholipids, and in terms of accessibility of cleavable sites present on the outer membrane surface for the proteins. treatment of phenylhydrazine-exposed erythrocytes either with bee venom phospho ... | 1989 | 2757386 |
| sensitivity of ocellar interneurons of the honeybee to constant and temporally modulated light. | sinusoidally modulated and discrete light pulses, the parameters of which approximated natural light conditions, were used to determine the response characteristics of ocellar first-order interneurons of the worker honeybee (apis mellifera carnica). large ocellar interneurons which terminate within the brain (lb neurons) were recorded from intracellularly and were identified visually after dye injection. absolute sensitivity of lb neurons to light flashes ranges from 4 x 10(9) quanta/cm2s (q) fo ... | 1989 | 2760607 |
| [cell adhesion in rat fibroblasts: effect of tumor promoters]. | in a study performed on transformed (sgs/3a) and normal syngeneic rat cells (fg/2) to identify the molecular mechanisms which regulate cell adhesion and contact inhibition in cell transformation, we investigated the effects of tumor promoters on cell to cell adhesion of rat fibroblasts. as tumor promoters we used 12-tetradecanoyl-phorbole-13-acetate (tpa) and the melittin, a polypeptide from bee venom, both substances capable of stimulating the neoplastic transformation. the intercellular adhesi ... | 1989 | 2775550 |
| analysis of the cdna for phospholipase a2 from honeybee venom glands. the deduced amino acid sequence reveals homology to the corresponding vertebrate enzymes. | a cdna expression library was constructed from worker bee venom glands and screened with an antibody against phospho lipase a2. the nucleotide sequence of a positive clone with the largest insert showed an open reading frame that codes for part of the signal peptide, the pro-region and the entire mature enzyme of the bee venom phospholipase a2 precursor. this sequence differs in the central region from the one determined by shipolini et al. [febs lett. 17, 39-40 (1971)] in showing, among other e ... | 1989 | 2776767 |
| a novel neurogenic vasodilator mechanism in bovine mesenteric artery. | the presence of a neurogenic vasodilator mechanism was investigated in isolated bovine mesenteric arteries (bmas) that were precontracted with phenylephrine. electrical field stimulation induced tetrodotoxin-sensitive relaxations in guanethidine-pretreated bmas. the relaxation occurred after a delay of about 5-8 seconds and amounted to 25-35% in different sets of experiments. the relaxation was not affected by classical receptor antagonists such as atropine (1 microm), cimetidine (3.9 microm), c ... | 1989 | 2791226 |
| a study of phospholipase a2-induced oedema in rat paw. | the inflammaogenic action of four extracellular phospholipases a2 was tested in the rat paw oedema model. subplantar injection of microgram amounts of the venom phospholipases a2 from vipera russeli, naja mocambique mocambique and honey bee, or the porcine enzyme produced a rapid but transient oedematous response. the venom enzyme from vipera was the most potent in this respect, the pancreatic enzyme the least. pretreatment of the enzymes with para-bromophenacylbromide profoundly inhibited the a ... | 1989 | 2806373 |
| acute toxicity and anti acetylcholinesterase potential of some biphenyl derivatives to non target species. | five newly synthesised biphenyl derivatives were evaluated for their acute contact toxicity (lc50) against rice weevil and honey bee and anti acetylcholinesterase potential (i50) against honey bee, fish, pigeon and rat. amongst, o,o-dimethyl-o, p-nitro-biphenyl phosphate was most potent against rice weevil, whereas p-(4-nitrophenyl) phenyl-n-methyl carbamate against honey bee. based on i50 values the biphenyl derivatives of phosphoric acid esters were more potent anti acetylcholinesterase (ache) ... | 1989 | 2807406 |
| cytochemical demonstration of increased phospholipid content in cell membranes in chlorphentermine-induced phospholipidosis. | we recently introduced a novel cytochemical approach to high-resolution cytochemistry of phospholipids in biological tissues. the technique consists of adsorption of bee venom phospholipase a2 to colloidal gold particles (pla2-gold complex) and subsequent application of this complex for localization of the enzyme substrate, i.e., glycerophospholipids. in the present study, this technique was applied at the post-embedding level, in both light (lm) and transmission electron microscopy (tem), to in ... | 1989 | 2911004 |
| interactions between discrete neuronal membrane binding sites for the putative k+-channel ligands beta-bungarotoxin, dendrotoxin and mast-cell-degranulating peptide. | 1. beta-bungarotoxin, a presynaptically active neurotoxin from the venom of bungarus multicinctus, was radiolabelled with 125i and its binding to synaptic membranes from rat brain was analyzed. the interaction of these binding sites with those for dendrotoxin (a convulsant polypeptide from mamba venom) and mast-cell-degranulating peptide (from bee venom) was examined in the light of the known effects of all three toxins on voltage-dependent k+ currents. 2. when measured in krebs/phosphate buffer ... | 1989 | 2912733 |
| k+ channels openers prevent epilepsy induced by the bee venom peptide mcd. | 1989 | 2920778 | |
| production of human antibodies to bee venom phospholipase a2 in vitro. | phospholipase a2 (pla) is the major antigen of bee venom. whereas individuals frequently stung by bees, such as bee keepers, show high levels of igg4 anti-pla antibodies in serum, most patients sensitive to bee venom possess increased ige anti-pla. we have established a culture system by which anti-pla antibodies can be induced in vitro. peripheral blood mononuclear cells were stimulated in a first step with pla and/or pokeweed mitogen (pwm). after 3 days of culture the cells were washed and fur ... | 1989 | 2922572 |
| deadly serious humour for the "go-go girls". thailand. | 13,600 people had been officially reported by 1989 as being infected with hiv in thailand. unofficial government projections, however, suggest that the true extent of hiv infection was closer to 30,000 cases, and that more than one million people will be infected by 1994. thousands of tourists visit thailand annually in search of sex. empower is an association established in 1985 to help the estimated 4000 women who work in patpong's bars and brothels. empower produces the honeybee cabaret, ... | 1989 | 12290134 |
| genetic polymorphisms and their relationships with inbreeding and breed structure in rare british sheep: the portland, manx loghtan, and hebridean. | 320 sheep of three rare british breeds were typed for blood group and biochemical polymorphism. genetic distance was least between the manx loghtan and hebridean (d = 0.1241, supporting historical evidence of a close relationship between the breeds. distances to the portland breed were 0.144 and 0.186, respectively. the claim that the portland is related to the dorset horn was substantiated by the fact that both breeds possess the i blood group allele. pedigree analysis showed that most rare or ... | 1989 | 21129024 |
| ultrastructural differences of neurosecretion granules in the corpora cardiaca of the honeybee with and without infection by nosema apis. | axons of corpora cardiaca in the nosema infected honeybees contained numerous tightly packed neurosecretion granules. these granules invariably possessed high electron densities. neurosecretion granules of similar size were also observed in the axons of corpora cardiaca in the healthy honeybees. but these granules were shown to possess various electron densities. image analysis revealed that some of these granules possessed an electron dense core, and the electron densities decreased gradually f ... | 1990 | 18620317 |
| ultrastructural changes in the secretion granules of the hypopharyngeal glands of the honeybee infected by nosema apis and after treatment with fumagillin. | electron-dense secretion granules were numerous in the hypopharyngeal glands of healthy honeybees. in the hyopharyngeal glands of honeybees infected by nosema apis, these granules appeared to have increased in size and lost their electron density, possessing a core area that consisted of numerous smaller granules, and a slightly electron dense fringe area, which in some cases possessed a crystalline structure. in the hypopharyngeal glands of infected honeybees after treatment with fumagillin, th ... | 1990 | 18620318 |
| ultrastructural analysis on the gland secretion in the extracellular ducts of the hypopharyngeal glands of the honeybee infected by nosema apis. | the hypopharyngeal glands of the honeybee secrete the larval food royal jelly, along with enzymes that are used in the production of honey from nectar. the composition of this secretion mixture varies with the physiological condition of the honeybee, and the final secretion mixture is conveyed through the extracellular ducts to the large excretory duct. the secretion products in the extracellular duct of both healthy and infected honeybees, was studied by transmission electron microscopy and ima ... | 1990 | 18620319 |
| apamin binding sites in human c6 cell line and their accessibility to monoclonal antibodies. | apamin, a 18-amino acid bee venom toxic peptide specifically blocks a class of ca(2+)-activated k+ channels. i) mono 125i-iodoapamin binds to receptor sites in a human neuroglial cell line (c6 line) but not in human cell lines from pancreatic (rin5f line) and colonic origin (ht29 line). ii) receptor-bound apamin is still accessible to a large molecule since some anti-apamin monoclonal antibodies recognize apamin when bound to its receptor, both in intact cells of the human c6 glioma line and in ... | 1990 | 1966607 |
| purification and pharmacological characterization of peptide toxins from the black mamba (dendroaspis polylepis) venom. | this paper reports the purification of 28 different peptides from the venom of the snake dendroaspis polylepis. these peptides represent 99% of the total peptide fraction in the venom. the 14 most cationic peptides form a structurally and functionally homogeneous group of analogs of the most abundant dendrotoxin toxin i (dtxi). they recognize antibodies raised against dtxi as well as brain membrane binding sites corresponding to k+ channels that are sensitive to dtxi and the bee venom peptide mc ... | 1990 | 1977212 |
| homeobox containing genes in the nematode caenorhabditis elegans. | we designed a unique 36-mer oligonucleotide probe, based on the most highly conserved amino acid sequences of antennapedia-like homeodomains and the codon bias of caenorhabditis elegans. this probe was then used to isolate four classes of genes from a c. elegans genomic library. sequencing reveals that we have isolated three new homeobox genes, designated ceh-1, ceh-9 and ceh-10. the fourth homeobox gene, ceh-11, has recently been described by schaller et al (nucleic acids res. 18, 2033-2036). t ... | 1990 | 1978282 |
| hemolytic potency and phospholipase activity of some bee and wasp venoms. | 1. the action of crude venoms of four aculeate species: apis mellifera, vespa crabro, vespula germanica and vespula vulgaris on human erythrocytes was investigated in order to determine the lytic and phospholipase activity of different aculeate venoms and their ability to induce red blood cell hemolysis. 2. bee venom was the only extract to completely lyse red blood cells at the concentration of 2-3 micrograms/ml. 3. phospholipase activity in all of the examined vespid venoms was similar and the ... | 1990 | 1981342 |
| rapid activation of the non-toxic basic isoform of phospholipase a2 from naja mossambica mossambica (spitting cobra) by long-chain fatty acylation. | purified phospholipase a2 from naja naja (common indian cobra) venom, a basic non-toxic isoform and a toxic isoform from naja mossambica mossambica venom were treated with an equimolar amount of oleoyl imidazolide, a known activator of bee venom phospholipase a2. the ability of the first two enzymes to induce erythrocyte lysis was strongly activated while hydrolysis of dioctanoyl phosphatidyl choline was only weakly activated. the toxic enzyme showed little change in either assay. the susceptibl ... | 1990 | 2080519 |
| projectin is an invertebrate connectin (titin): isolation from crayfish claw muscle and localization in crayfish claw muscle and insect flight muscle. | a filamentous protein was isolated from crayfish claw muscle. this protein had physiochemical properties very similar to vertebrate skeletal muscle connectin (titin), although its apparent molecular mass (approximately 1200 kda) was considerably lower than that of connectin (approximately 3000 kda). polyclonal as well as monoclonal antibodies against chicken skeletal muscle connectin reacted with the 1200 kda protein from crayfish claw muscle. conversely, polyclonal antibodies against crayfish 1 ... | 1990 | 2084147 |
| antibiotic activity of natural products: 1. propolis. | material extracted from propolis (bee glue) by alkaline aqueous solvents or organic solvents showed weak inhibitory activity in vitro against certain species of gram-positive bacteria. no antimicrobial activity was detected in urine from three volunteers who had taken 500 mg propolis three times a day for 3 days. | 1990 | 2110610 |
| purification and characterization of honey bee vitellogenin. | a protocol has been developed for the purification of vitellogenin from the honey bee, apis mellifera. purification allows for the first characterization of a vitellogenin from the large order hymenoptera. hymenopteran vitellogenins are unusual among insect vitellogenins in that they contain only one type of apoprotein. the honey bee vitellogenin was isolated from hemolymph of honey bee queens by a combination of density gradient ultracentrifugation, ion-exchange chromatography, and affinity chr ... | 1990 | 2134180 |
| a method to generate antigen-specific suppressor t cells in vitro from peripheral blood t cells of honey bee venom-sensitive, allergic patients. | peripheral blood mononuclear cells of patients allergic to honey bee venom were stimulated with denatured bee venom phospholipase a2, and the antigen-activated t cells were propagated for 4 days by human il-2 in the presence or absence of recombinant human lipocortin i. upon antigenic stimulation with the denatured phospholipase a2 and autologous monocytes or by cross-linking of cd3 by anti-cd3 antibody, the activated t cells, which had propagated by il-2 alone, formed n-glycosylated ige-binding ... | 1990 | 2138201 |
| rhodopsin reconstitution in bleached rod outer segment membranes in the presence of a retinal-binding protein from the honeybee. | the physiological role of a retinal-binding protein from honeybee is investigated. this protein, upon previous loading with all-trans retinal and subsequent irradiation with monochromatic light of wavelength 490 nm, is able to promote the reconstitution of rhodopsin when added to a suspension of opsin membranes from bleached bovine rod outer segments. in this respect this retinal-binding protein could have a role very similar to that postulated for the well-known cephalopod retinochrome, that se ... | 1990 | 2143484 |
| [125i]azidosalicylyl melittin binding domains: evidence for a polypeptide receptor on the gastric (h+ + k+)atpase. | we have previously shown that melittin, a bee venom peptide, potently inhibited the catalytic and transport functions of rabbit gastric (h+ + k+)atpase. a radioactive photoaffinity analog of melittin, ([125i]azidosalicylyl melittin), labeled the (h+ + k+)atpase. these results suggested that melittin exerted inhibitory effects through direct interaction with the (h+ + k+)atpase. in this study we attempt to define the melittin-binding domain of the (h+ + k+)atpase using conformation-dependent prot ... | 1990 | 2158280 |
| phospholipids chiral at phosphorus. stereochemical mechanism of reactions catalyzed by phosphatidylinositide-specific phospholipase c from bacillus cereus and guinea pig uterus. | (rp)- and (sp)-1,2-dipalmitoyl-sn-glycero-3-thiophosphoinositol (dppsi) were synthesized as a mixture and their configurations assigned on the basis of the stereospecific hydrolysis catalyzed by phospholipase a2 (pla2) from bee venom. pla2 is known to be stereospecific to the rp isomer of 1,2-dipalmitoyl-sn-glycero-3-thiophosphocholine (dppsc) and 1,2-dipalmitoyl-sn-glycero-3-thiophosphoethanolamine (dppse). since the configurations of (rp)- and (sp)-dppsi correspond to those of (sp)- and (rp)-d ... | 1990 | 2161255 |
| effect of staphylococcal delta-toxin and bee venom peptide melittin on leukotriene induction and metabolism of human polymorphonuclear granulocytes. | the abilities of delta-toxin from staphylococcus aureus and melittin to induce and modulate the generation of leukotriene from human polymorphonuclear granulocytes (pmns) were studied. stimulation of pmns with melittin (10 micrograms) induced leukotriene formation, whereas stimulation with delta-toxin did not. preincubation of the pmns with delta-toxin modulated the subsequent generation of leukotriene from pmns induced by ca ionophore a23187 or opsonized zymosan. the generation of leukotriene b ... | 1990 | 2164512 |
| long-term enhancement of synaptic transmission by synthetic mast cell degranulating peptide and its localization of binding sites in hippocampus. | a bee venom, mast-cell-degranulating (mcd) peptide, was synthesized by stepwise formation of the two disulfide bridges. this synthetic mcd peptide induced long-term potentiation (ltp) in the ca1 region of a hippocampus slice at concentrations ranging from 10(-7) to 10(-5) m. the potentiating effect of mcd was not lost by biotinylation of its n-terminus, and thus it became possible to investigate localization of its binding site at a cellular level in the hippocampus slice at a ltp-inducible conc ... | 1990 | 2170876 |
| bee sting-induced haemolysis, spherocytosis and neural dysfunction in three dogs. | three dogs showed signs of intravascular haemolysis, spherocytosis and peripheral neuropathy following severe bee envenomation. these effects were ascribed to the constituents of bee venom, especially melittin and phospholipase a. all 3 cases recovered and were discharged subsequent to prednisolone and supportive therapy. | 1990 | 2176693 |
| modulation of dl-alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (ampa)/quisqualate receptors by phospholipase a2 treatment. | the expression of long-term potentiation (ltp) in area ca1 of hippocampus has been proposed to result from an increased sensitivity of the ampa/quisqualate receptors. we have investigated the binding properties of excitatory amino acid receptors in phospholipase a2 (pla2)-treated rat brain membranes. pla2 from bee venom produced a significant increase in the binding of [3h]-ampa ([3h]-amino-3-hydroxy-5-methylisoxazole-4- propionate), a ligand for the ampa/quisqualate receptor. analysis of the sa ... | 1990 | 2177177 |
| interaction of melittin with the (na+ + k+)atpase: evidence for a melittin-induced conformational change. | the (na+ + k+)atpase is inhibited by the bee venom polypeptide, melittin. kcl and nacl protect the enzyme from melittin inhibition. analysis of the k+ and na+ protection against melittin inhibition suggested a kinetic model which was consistent with slowly reversible melittin binding, and mutually exclusive binding of melittin with k+ and na+. accordingly, in the absence of salt, the ki for melittin inhibition = 1.2 microm, and the protection by kcl occurs with a ka,kcl = 0.6 mm. the protection ... | 1990 | 2177321 |
| antibacterial properties of propolis (bee glue). | propolis (bee glue) was found to have antibacterial activity against a range of commonly encountered cocci and gram-positive rods, including the human tubercle bacillus, but only limited activity against gram-negative bacilli. these findings confirm previous reports of antimicrobial properties of this material, possibly attributable to its high flavonoid content. | 1990 | 2182860 |
| quantity, analysis, and lethality of european and africanized honey bee venoms. | venom from africanized honey bees (derived mainly from apis mellifera scutellata) was compared with venom from domestic, european bees by study of lethality, immunological cross-reactivity, venom yield, isoelectric focusing (ief) patterns, and melittin titers. the ld50s of european and africanized bee venom by iv injection in mice were similar. in venom neutralization experiments, africanized bee venom was mixed with antibodies from a beekeeper exposed only to european bees and used to challenge ... | 1990 | 2200291 |
| interaction of wasp venom mastoparan with biomembranes. | mastoparan-induced changes in the k+ permeability of rat peritoneal mast cells, human erythrocytes, staphylococcus aureus and escherichia coli were examined. mastoparan did not efficiently increase the k+ permeability of cells except for s. aureus. the release of membrane phospholipids was also observed from s. aureus cells in the concentration range of the permeability enhancement. mastoparan stimulated histamine release from mast cells, independently of a small efflux of k+. mastoparan became ... | 1990 | 2204429 |
| characterization of a promoter and a transcription terminator of spiroplasma melliferum virus spv4. | spiroplasma virus 4 (spv4) is an isometric virus with single-stranded, circular dna infecting the helical mollicute spiroplasma melliferum, a honeybee pathogen. previous studies in our laboratory led to the determination of the base sequence of the spv4 dna. nine open reading frames and three promoterlike sequences (p1, p2, and p3) were identified. an inverted repeat leading to the formation of a hairpin structure on the transcription product was also found and predicted to be a transcription te ... | 1990 | 2211498 |
| [experimental studies of in vitro cultivation of the cells of kärtner honeybees (apis mellifera carnica pollmann, 1879)]. | a synopsis about published methods and results on experiments to cultivate bee cells in vitro is given. experimental investigations were performed with haemocytes of larvae of the l-5 stage using many different media and methods for the preparation of primary tissue culture. monolayers could be prepared and a high rate of reproduction has been achieved, although subpassages could not be obtained. haemocytes could be kept alive up to 27 days by using bml-tc/7a medium according to gardiner and sto ... | 1990 | 2220184 |
| acyl carrier protein interacts with melittin. | acyl carrier protein (acp) from escherichia coli has been shown to form complexes with melittin, a cationic peptide from bee venom. acp is a small (mr 8847), acidic, ca2(+)-binding protein, which possesses some characteristics resembling those of regulatory ca2(+)-binding proteins including interaction with melittin. complexing between melittin and acp which occurred both in the presence and absence of ca2+ was evident by chemical cross-linking the two peptides, fluorescence changes (including a ... | 1990 | 2223852 |
| tryptase, a mediator of human mast cells. | tryptase, a mediator secreted by human mast cells during immediate reactions, has demonstrated effects on several pathways in vitro. this enzyme can rapidly inactivate fibrinogen and, as a complex with heparin, may prevent coagulation that may otherwise occur when plasma enters tissues at sites of immediate reactions. tryptase may also activate prostromelysin, which in turn activates latent collagenase. when canine pulmonary smooth muscle is incubated with canine tryptase, the contractile respon ... | 1990 | 2229822 |
| effect of melittin on renin and prostaglandin e2 release from rat renal cortical slices. | 1. the present experiments were designed to determine the effect of melittin on renin secretion. melittin is a polypeptide component of bee venom which stimulates phospholipase a2 activity, thereby increasing arachidonic acid release and prostaglandin (pg) synthesis, and which inhibits protein kinase c activity. either of these actions might be expected to stimulate renin secretion, since renin secretion is stimulated by arachidonic acid and by several pgs, and since renin secretion is inhibited ... | 1990 | 2231411 |
| antioxidant activity of and interleukin production affected by honey bee venom. | honey bee venom is found to inhibit significantly nonenzymatic lipid peroxidation. it also possesses a considerable hydroxyl radical scavenging activity, evaluated by its competition with dimethyl sulfoxide for ho.. these results, in relation to the in vitro suppression mainly of interleukin-1 production offered by honey bee venom, may further support that antioxidant activity is involved in the anti-inflammatory activity of honey bee venom. | 1990 | 2242083 |
| modulation of the immune response to allergens: phospholipase a degradation products suppress igg and ige response in mice. | degradation products of phospholipase a2 (pla2), the major allergen of honeybee venom, were prepared by peptic digestion of the enzyme and fractionated by molecular exclusion chromatography. the largest of the isolated products, p-1, was found to be a centrally nicked pla2 molecule with a loss of several amino acids. it was examined for its antigenic, immunogenic and immunosuppressive properties. this peptic product was unable to block antigen-antibody reactions between pla2 and anti-pla2 antibo ... | 1990 | 2246080 |
| allergy to stinging and biting insects in queensland. | over an eight and a half year period 742 patients were assessed for allergy to stinging and biting insects in queensland; 452 (61%) had allergic reactions to honey bees, 244 (33%) to wasps, 30 (4%) to various ants, 11 (1.5%) to march flies (tabanus sp.) and five to tick infestation. one hundred and fifty one patients (20%) presented with large local swelling only (rxn1), 98 (13%) with urticaria and/or facial angioedema distant from the sting site (rxn2) and 492 (66%) with subjective or objective ... | 1990 | 2246984 |
| inhalation injury to tracheal epithelium in an ovine model of cotton smoke exposure. early phase (30 minutes). | the purpose of this study was to evaluate lung cell injury during the acute phase of smoke inhalation injury. a group of 10 sheep were anesthetized with halothane and pancuronium followed by endotracheal intubation. in the first experiment 5 sheep were given air (sham group) and 5 were insufflated with cooled cotton smoke with a modified bee smoker. in the second part of our study (experiment 2) the animals were insufflated with the following number of smoke breaths: 1 x 12 (n = 3); 2 x 12 (n = ... | 1990 | 2252264 |
| heat-induced alterations in monkey erythrocyte membrane phospholipid organization and skeletal protein structure and interactions. | rhesus monkey erythrocytes were subjected to heating at 50 degrees c for 5-15 min, and the heat-induced effects on the membrane structure were ascertained by analysing the membrane phospholipid organization and membrane skeleton dynamics and interactions in the heated cells. membrane skeleton dynamics and interactions were determined by measuring the tris-induced dissociation of the triton-insoluble membrane skeleton (triton shells), the spectrin-actin extractability at low ionic strength, spect ... | 1990 | 2261489 |
| [injury of the cornea by a bee sting complicated by pseudomonas aeruginosa and herpesvirus infections]. | 1990 | 2264238 | |
| isolation of a phospholipase-a2-like protein from human fetal intestine. lysis of erythroid cells from bovine liver by this protein and porcine phospholipase a2. | extracts of human fetal intestine contain factors that can stimulate or inhibit thymidine incorporation into fetal bovine erythroid cells. an inhibitory factor was purified to homogeneity by gel-permeation and reversed-phase high performance liquid chromatography. the inhibitory action was due to cell lysis. the first 25 amino acids of the n-terminal segment were identical to the human lung and pancreatic phospholipase a2. the isolated protein released arachidonic acid from 2-arachidonyl phospha ... | 1990 | 2268412 |
| selective inhibition of cecropin-like activity of insect immune blood by protease from american foulbrood scales. | bioassays of american foulbrood larval scale filtrates have shown the presence of an immune inhibitor with a specific activity of proteases that selectively destroy cecropin-like activity in insect immune hemolymphs. it was an unexpected phenomenon to find that bacillus larvae protease(s), even at trace concentrations, totally inhibits bactericidal activity of immune blood against escherichia coli d 31. thermal inactivation of a proteolytic enzyme coincides strictly with a disappearance of the a ... | 1990 | 2273283 |
| [effects of bee pollen on lipid peroxides and immune response in aging and malnourished mice]. | the results showed that the level of hemolysin (hc), the numbers of plaque forming cells (pfc) and specific rosette forming cells (srfc) in primary response to sheep red blood cells (srbc) were markedly lowered and the lipoperoxide level in brain, liver and serum was increased in aging (over 18 months) and malnourished mice fed with ground corn in comparison to normal controls, while hc and the numbers of pfc and srfc were significantly increased and the lipoperoxide level was markedly decreased ... | 1990 | 2275784 |
| identification and partial purification of embryonic mouse genital protein(s) stimulating phospholipase-a2 and inducing masculinization in vitro. | the present study was designed to test whether phospholipase-a2 stimulatory protein (plsp) has any role during androgen-induced masculine differentiation. thus, an investigation was made to identify such a protein in the fetal genital tract and to test whether this protein can produce masculinization in vitro. fetal tracts (15/batch) containing genital ducts and urogenital sinus from male, female, and testosterone-exposed (40 mg/kg.day, from days 13-17 of gestation) female embryonic mice on day ... | 1990 | 2293992 |
| impact of carbon monoxide on cardiopulmonary dysfunction after smoke inhalation injury. | with the inhalation of smoke, there are both cardiopulmonary changes and elevated levels of carbon monoxide (co). we hypothesize that these changes in cardiopulmonary function are the result of a histotoxic hypoxia associated with co poisoning. this hypothesis was tested in chronically instrumented sheep (n = 19). piezoelectric crystals were attached to the left ventricle for the measurement of its external minor and major diameters in addition to wall thickness. a pressure transducer was placed ... | 1990 | 2295145 |
| isolation and characterization of abaecin, a major antibacterial response peptide in the honeybee (apis mellifera). | honeybee (apis mellifera) are frequently exposed to and likely to be infected by plant-associated bacteria. we mimicked this process by injecting bees with live bacteria and isolated five induced antibacterial substances by comparative liquid chromatographic mapping of the hemolymph. three of these antibiotics belong to a unique family of small (18 amino acids) peptides: the apidaecins [casteels et al. (1989) embo j. 8, 2387-2391]. we have now characterized a fourth bee immune response peptide. ... | 1990 | 2298215 |
| honey bee mortality due to tracheal mite parasitism. | we demonstrate, by truncating the expected negative binomial distribution, that the tracheal mite parasite, acarapis woodi, causes mortality in the european honey bee, apis mellifera, but, that this mortality can be documented only during periods of low mite densities (mites per bee). at high mite densities, this technique no longer reveals mite-induced mortality. we suggest that this paradox results from a reduction in the mortality threshold at high mite densities, concealing mortality from ou ... | 1990 | 2314929 |
| membrane skeleton-bilayer interaction is not the major determinant of membrane phospholipid asymmetry in human erythrocytes. | transbilayer phospholipid distribution, membrane skeleton dissociation/association, and spectrin structure have been analysed in human erythrocytes after subjecting them to heating at 50 degrees c for 15 min. the membrane skeleton dissociation/association was determined by measuring the tris-induced dissociation of triton-insoluble membrane skeletons (triton shells), the spectrin-actin extractability under low ionic conditions, and the binding of spectrin-actin with normal erythrocyte membrane i ... | 1990 | 2317498 |
| anti-inflammatory activity of bee venom peptide 401 (mast cell degranulating peptide) and compound 48/80 results from mast cell degranulation in vivo. | 1. the relationship between the anti-inflammatory activity of the bee venom peptide 401 in the carrageenin-induced oedema of the rat hind paw and its mast cell degranulating activity has been reinvestigated. 2. mast cell degranulation caused by compound 48/80 (10 mg kg-1) or by allergen challenge in rats sensitized to nippostrongylus brasiliensis also suppressed rat hind paw oedema in the same test. 3. the anti-inflammatory activities of peptide 401 and compound 48/80 were partially suppressed b ... | 1990 | 2328399 |
| [development of infestation with varroa jacobsoni o. in bee colonies in tunisia]. | the mite varroa jacobsoni, an ectoparasite of the honey bee, was imported to tunisia probably in 1976. afterwards, this parasitosis caused severe losses of colonies for several years. the continued examination of the level of infestation in colonies of a "gtz" project stated a steady number of mites since 1980. only in a few colonies, the infestation was above the limit of damage. though the colonies in north west tunisia did not receive any treatment since 1986 there was no increase of infestat ... | 1990 | 2331244 |
| [changes in the immunological reactivity of patients with disseminated sclerosis treated by prednisolone and the preparation proper-myl]. | the authors examined 56 patients suffering of multiple sclerosis who were treated with prednisolone and proper-myl. the patients showed changes of the immunological reactivity, in particular, those receiving prednisolone in combination with proper-myl. to improve the immunological state of these patients the authors recommend to use bee stings, polyosol and bee pollen. | 1990 | 2339565 |
| effect of staphylococcus aureus delta-toxin on human granulocyte functions and platelet-activating-factor metabolism. | the production of delta-toxin is supposed to be responsible for various pathophysiological effects during infection with staphylococcus aureus. we compared the effects of delta-toxin with the structurally related bee venom toxin melittin on granulocyte functions and inflammatory mediator release. delta-toxin and melittin induced a rapid ca2+ influx, as was shown by fluorescence detection. furthermore, oxygen radical production, as determined by luminol-enhanced chemiluminescence, was triggered b ... | 1990 | 2341170 |
| juvenile hormone titer in capped worker brood of apis mellifera and reproduction in the bee mite varroa jacobsoni. | juvenile hormone (jh) titers were recorded from fifth instar worker larvae of apis mellifera carnica, apis mellifera lamarckii, and africanized honeybees kept under temperate and tropical climatic conditions. no differences in hormone titer according to honeybee race or climatic conditions were determined. however, the rate of reproduction of the ectoparasitic mite, varroa jacobsoni, on larvae of the different honeybee races was highly variable. the possible role of honeybee jh in control of the ... | 1990 | 2354762 |
| a potent antibacterial protein in royal jelly. purification and determination of the primary structure of royalisin. | a new potent antibacterial protein, for which we propose the name royalisin, was found in royal jelly of the honeybee apis mellifera l. and purified to homogeneity for the first time by acid extraction, gel filtration, and reverse-phase high pressure liquid chromatography. the primary structure of royalisin was determined to consist of 51 residues, with three intramolecular disulfide linkages, having a calculated molecular mass of 5523 da. royalisin is an amphipathic protein, with the c-terminal ... | 1990 | 2358464 |
| mast cell degranulating peptide induces the expression of the c-fos proto-oncogene in hippocampus. | the intrahippocampal injection of the mast cell degranulating (mcd) peptide, a bee venom component acting on the k+ channel, results in the appearance of the proto-oncogene c-fos mrna in the ipsi- and contralateral hippocampus of the treated animals without generating convulsions. this mcd-induced transcriptional event is discussed in terms of cellular plasticity since mcd peptide is known to induce long-term potentiation. | 1990 | 2365001 |
| effects of cold shock and phospholipase a2 on intact boar spermatozoa and sperm head plasma membranes. | head plasma membranes (hpm) isolated from cryopreserved boar spermatozoa show an excessive fluidization, which might be involved in the loss of fertility. the current study assessed the ability of cold shock (5 degrees c) and phospholipase a2 (pa2) to duplicate these effects on membrane structure and to affect 45ca2+ uptake and gross morphological characteristics of whole, fresh boar-sperm. the hpm from cold-shocked sperm showed a significantly greater rate of fluidization over time than did hpm ... | 1990 | 2372397 |
| folding and activity of hybrid sequence, disulfide-stabilized peptides. | peptides have been synthesized that have hybrid sequences, partially derived from the bee venom peptide apamin and partially from the s peptide of ribonuclease a. the hybrid peptides were demonstrated by nmr spectroscopy to fold, forming the same disulfides and basic three-dimensional structure as native apamin, containing a beta-turn and an alpha-helix. these hybrids were active in complementing s protein, reactivating nuclease activity. in addition, the hybrid peptide was effective in inducing ... | 1990 | 2377603 |
| epitope mapping of apamin by means of monoclonal antibodies raised against free or carrier-coupled peptide. | a panel of 20 monoclonal antibodies raised against the bee-venom peptide apamin (18 residues, 2 disulfide bridges) was prepared. nine monoclonal antibodies (mab) were obtained from a mouse immunized with free apamin and 11 from a mouse immunized with a mixture of free and carrier-coupled peptide. using a panel of 11 synthetic apamin analogs, we examined the fine antigenic specificity of each antibody. the mab generated against free apamin preferentially bound to the central part of the peptide a ... | 1990 | 1696354 |
| ultrastructural localization of prolactin-like antigenic determinants in neurosecretory cells in the brain of the honeybee (apis mellifica). | with two different antisera to human prolactin (hprl), the ultrastructural localization of prl-like material in the bee brain is examined by means of the protein-a-gold method at the electron microscopical level. labelling is found in electron-dense granules of medium size (150-200 nm in diameter) for the first time in insects. such granules are distributed in the cytoplasm of the neurosecretory cells, their axons and their axon-terminals. the electron-dense granule is one criterion for identify ... | 1990 | 1699860 |
| calcium-activated potassium channels in basolateral membranes of colon epithelial cells; reconstitution and functional properties. | using differential sedimentation, isopycnic and ficoll-400 barrier centrifugation, basolateral membrane vesicles of surface and crypt cells of the rabbit distal colon were enriched 34- and 9-fold, respectively. 86rb(+)-uptake into these vesicles, driven by an electrical potential difference, was stimulated by submicromolar ca2+ activities and inhibited by ba2+. these findings indicate the presence of ca2(+)-activated k+ channels. the k+ channels in surface and crypt cell membranes differed with ... | 1990 | 1701591 |
| mast cell degranulating peptide: a multi-functional neurotoxin. | this review discusses our present knowledge of the structure and activities of the mast cell degranulating peptide (mcdp). this peptide is a basic, 22 amino acid residue component of honey bee venom with striking immunological and pharmacological activities. mcdp is a potent anti-inflammatory agent, but at low concentrations it is a strong mediator of mast cell degranulation and histamine release. mcdp is also an epileptogenic neurotoxin, an avid blocker of the potassium channels and can cause a ... | 1990 | 1703229 |
| whole body extract versus venom treatment in anaphylactic reactions to bee stings: the doctor's dilemma. | 1990 | 267782 | |
| apis mellifera cytoplasmic elongation factor 1 alpha (ef-1 alpha) is closely related to drosophila melanogaster ef-1 alpha. | using low stringency hybridisation with a drosophila melanogaster ef-1 alpha gene fragment we have isolated a genomic dna clone encoding elongation factor 1 alpha (ef-1 alpha) from apis mellifera. the hybridising apis mellifera sequence could be delineated to two small ecori fragments that were also revealed by genomic southern hybridisation. by comparison with the corresponding drosophila melanogaster data the complete translational reading frame has been deduced. it is interrupted by two inter ... | 1990 | 2116322 |
| transbilayer redistribution of phosphatidylserine in erythrocytes of a patient with autoerythrocyte sensitization syndrome (psychogenic purpura). | we have investigated a female patient with autoerythrocyte sensitization syndrome (aes syndrome), having a positive skin response to her own red blood cells (rbc) and to phosphatidylserine (ps). using 2,4,6-trinitrobenzenesulfonic acid (tnbs), bee venom phospholipase a2 and merocyanine 540 binding, we have demonstrated that in rbc of patient more than 50% of ps is redistributed into the outer leaflet of the plasma membrane. using homologous rbc from a healthy donor we were able to induce transbi ... | 1990 | 1713890 |
| crystal structure of bee-venom phospholipase a2 in a complex with a transition-state analogue. | the 2.0 angstroms crystal structure of a complex containing bee-venom phospholipase a2 (pla2) and a phosphonate transition-state analogue was solved by multiple isomorphous replacement. the electron-density map is sufficiently detailed to visualize the proximal sugars of the enzyme's n-linked carbohydrate and a single molecule of the transition-state analogue bound ot its active center. although bee-venom pla2 does not belong to the large homologous class i/ii family that encompasses most other ... | 1990 | 2274788 |
| crystal structure of cobra-venom phospholipase a2 in a complex with a transition-state analogue. | the crystal structure of a complex between a phosphonate transition-state analogue and the phospholipase a2 (pla2) from naja naja atra venom has been solved and refined to a resolution of 2.0 angstroms. the identical stereochemistry of the two complexes that comprise the crystal's asymmetric unit indicates both the manner in which the transition state is stabilized and how the hydrophobic fatty acyl chains of the substrate are accommodated by the enzyme during interfacial catalysis. the critical ... | 1990 | 2274787 |
| interfacial catalysis: the mechanism of phospholipase a2. | a chemical description of the action of phospholipase a2 (pla2) can now be inferred with confidence from three high-resolution x-ray crystal structures. the first is the structure of the pla2 from the venom of the chinese cobra (naja naja atra) in a complex with a phosphonate transition-state analogue. this enzyme is typical of a large, well-studied homologous family of pla2s. the second is a similar complex with the evolutionarily distant bee-venom pla2. the third structure is the uninhibited p ... | 1990 | 2274785 |
| serum carnitine levels in bone marrow transplant recipients. | this study investigated plasma carnitine levels in patients undergoing allogenic bone marrow transplantation. the patients received fat-based tpn (50% fat, 50% cho; calorie: nitrogen ratio 125:1) for an average of 33 +/- 7.5 days. tpn was started before transplantation and stopped when patients were able to eat. caloric needs were estimated using the harris-benedict equation; 150% of the estimated bee was given for the first two weeks after transplantation. the amount of tpn was gradually decrea ... | 1990 | 16837382 |
| acute renal failure due to multiple bee stings--case reports. | this report deals with a father and his son who developed acute renal failure following multiple bee stings. the renal lesion in these patients appears to be due to rhabdomyolysis caused by the bee venom. the other mechanisms are also discussed. the need for clinicians to be aware of acute renal failure as a complication of bee stings is stressed. | 1990 | 2152058 |
| determination of n-methylhistamine in urine as an indicator of histamine release in immediate allergic reactions. | the use of the urine histamine metabolite, n-methylhistamine (n-mh), as a parameter of histamine release in immediate allergic reactions was investigated. baseline levels were determined in 34 normal control subjects and 29 atopic patients. increases of urine n-mh values were measured during histamine infusions and in venom-allergic patients receiving bee-sting challenges. n-mh was determined by a newly developed radioimmunoassay. baseline levels in control subjects and atopic patients demonstra ... | 1990 | 1702126 |
| quantitative autoradiographic localization of [125i]alpha-bungarotoxin binding sites in the honeybee brain. | binding sites for the nicotinic acetylcholine receptor antagonist, [125i]alpha-bungarotoxin, were localized in the honeybee brain by in vitro autoradiography. highest binding site densities were localized in the suboesophageal ganglion, the optic tubercles, optic lobes medulla and lobula, antennal lobes, dorsal lobes and the alpha-lobes of the mushroom bodies. the distribution pattern of these putative nicotinic acetylcholine receptors suggests that acetylcholine is involved in several sensory p ... | 1990 | 2073597 |
| behavioral responses by bumble bees to variation in pollen availability. | pollen-collecting bumble bees (bombus spp.) detect differences between individual flowers in pollen availability and alter their behavior to capitalize on rewarding flowers. specific responses by bees to increased pollen availability included: longer visits to flowers; visits to more flowers within an inflorescence, including an increased frequency of revisits; an increased likelihood of grooming while the bee flow between flowers within the inflorescence; and more protracted inter-flower flight ... | 1990 | 28310953 |
| chemicals involved in honeybee-sunflower relationship. | we present a review of work on the plant chemicals involved in the honeybee-sunflower model system. combined behavioral and chemical analyses were conducted under natural and controlled conditions. first the distribution of forager bees' visits on two pairs of sunflower genotypes producing a different level of hybrid seed yield was recorded under pollen-proof tunnels. mirasol parental lines producing high seed yields were visited at random, whereas forager bees visited preferentially the female ... | 1990 | 24263296 |
| protection against membrane damage: a 1h-nmr investigation of the effect of zn2+ and ca2+ on the permeability of phospholipid vesicles. | 1h-nmr spectroscopy of small, unilamellar dipalmitoyl phosphatidylcholine (dppc) vesicles in conjunction with the lanthanide shift reagent pr3+ was used to study the effect of zn2+ and ca2+ ions on the permeability induced at the lipid phase transition temperature (tc) of the vesicles and by the bee venom polypeptide melittin. in addition, the effects of zn2+ and ca2+ were studied on triton x-100 stabilized channels at tc and in the presence of n-alcohols. the results show that the presence of 1 ... | 1990 | 2292682 |
| honey bee cognition. | the visual memory of honey bees is stored pictorially. bees will accept a mirror-image reversal of a familiar pattern in the absence of the original, but prefer the original over the reversal; the matching system of bees, therefore, does not incorporate a mirror-image ambiguity. bees will not accept a rotation of a familiar vertical pattern, but readily recognize any rotation of a horizontal pattern; the context-specific ability to make a mental transformation seems justified by natural continge ... | 1990 | 2269009 |
| prediction of 24-h energy expenditure and its components from physical characteristics and body composition in normal-weight humans. | the applicability of body composition as estimated by the bioimpedance method to predict energy expenditure (ee) was studied. ten healthy subjects underwent measurement of body composition and 24-h energy expenditure (24-h ee) twice in a respiration chamber on a fixed program. the 24-h ee and its components, sleeping ee (see), basal ee (bee), and daytime ee, for an individual were very reproducible (coefficient of variation 2.3%, 1.4%, 5.0%, & 3.1%, respectively). the variability of 24-h ee amon ... | 1990 | 2239751 |
| the hymenoptera venom study. iii: safety of venom immunotherapy. | one thousand four hundred ten (44%) of the 3236 subjects in the hymenoptera venom study accepted venom immunotherapy (vit). time to maintenance averaged 95 days, and the largest number achieved maintenance (147 subjects, 10.4%) at day 56. ninety-two percent of the treated subjects achieved maintenance, and 84% continued therapy, most subjects (91%) until the study was terminated. one hundred seventy-one subjects (12%) experienced 327 treatment systemic reactions (srs). the incidence of pruritus ... | 1990 | 2229842 |
| analysis of meteorological and radiological data for selected fallout episodes. | the weather service nuclear support office has analyzed the meteorological and radiological data collected for the following atmospheric nuclear tests: trinity; easy of the tumbler-snapper series; annie, nancy, badger, simon, and harry of the upshot-knothole series; bee and zucchini of the teapot series; boltzmann and smoky of the plumbbob series; and small boy of the dominic ii series. these tests were chosen as having the greatest impact on nearby downwind populated locations, contributing app ... | 1990 | 2211117 |
| total energy expenditure in burned children using the doubly labeled water technique. | total energy expenditure (tee) was measured in 15 burned children with the doubly labeled water technique. application of the technique in burned children required evaluation of potential errors resulting from nutritional intake altering background enrichments during studies and from the high rate of water turnover relative to co2 production. five studies were discarded because of these potential problems. tee was 1.33 +/- 0.27 times predicted basal energy expenditure (bee), and in studies where ... | 1990 | 2171351 |
| the bee: an unusual gastric foreign body. | 1990 | 2242750 | |
| [morphological observation of effect of bee pollen on intercellura lipofuscin in nih mice]. | by histochemical method we have observed the effect of bee pollen on lipofuscin in nih mice. the results show that oral administration of bee pollen at 10 g/kg/d markedly reduces lipofuscin in cardiac muscle in aged mice, significantly inhibits the increase of lipofuscin in cardiac muscle, liver, brain and adrenal gland cells induced by orally given peroxidized corn oil or iv alloxan. this action may be related with the anti-ageing effect of bee pollen. | 1990 | 2092719 |
| randomized trial of postoperative patient-controlled analgesia vs intramuscular narcotics in frail elderly men. | postoperative use of as-needed intramuscular narcotics is potentially hazardous in frail elderly patients. patient-controlled analgesia (pca) allows patients to self-administer small boluses of narcotic, allowing better dose titration, enhanced responsiveness to variability in narcotic requirements, and reduction in serum narcotic level fluctuation. although theoretically useful, this method has not bee well studied in the elderly or medically ill. a prospective controlled trial among 83 higher- ... | 1990 | 1975490 |
| preference of cabbage white butterflies and honey bees for nectar that contains amino acids. | amino acids occur in most floral nectars but their role in pollinator attraction is relatively unstudied. nectars of butterfly-pollinated flower tend to have higher concentrations of amino acids than do flowers pollinated by bees and many other animals, suggesting that amino acids are important attractants of butterflies to flowers. in order to determine whether amino acids are important in attracting butterflies and bees, we tested the preference of cabbage white butterflies (pieris rapae) and ... | 1990 | 28312774 |
| acute renal failure and dermal necrosis due to bee stings: report of a case in a child. | although several cases of reactions to bee venom including acute renal failure have been reported, the literature from africa is scanty and that concerning children virtually non-existent. we report a child who was stung by over 1,000 bees and developed acute renal failure and extensive scalp necrosis. the pathogenesis of these complications is discussed and the importance of their public awareness stressed. | 1990 | 2282653 |
| proposed solution structure of endothelin. | a model is proposed for the 3-dimensional structure of endothelin, a potent vasoconstrictor and pressor peptide from vascular endothelium. the model is derived through protein structure prediction and circular dichroism studies, and is based on the atomic coordinates for the bee-venom peptide apamin. the model derived shows the same turn-helix motif as observed for apamin and mast-cell degranulating peptide. on the basis of this model we suggest possible strategies for endothelin antagonist desi ... | 1990 | 2272749 |