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virulence of entamoeba histolytica strains of human origin in bombay to golden hamster.the virulence following intrahepatic inoculation to hamsters with 17 strains of entamoeba histolytica isolated in cultures from clinical cases of amoebiasis and from asymptomatic carriers has been investigated. all 10 strains from clinical cases and four from asymptomatic carriers were found to be virulent for hamster liver, producing moderate or high grade of hepatic lesions, whereas three strains from asymptomatic carriers were found to possess low virulence with production of low grade of liv ...19846094892
[seroepidemiology of entamoeba histolytica infection in chile]. 19846095390
use of the enzyme-linked immunosorbent assay (elisa) for detection of entamoeba histolytica antigen in faecal samples.a promising new elisa method developed by root et al. to diagnose amoebiasis by the detection of entamoeba histolytica coproantigen was tested in mexico city and was reported to be more than twice as sensitive as microscopy. in this study the same procedure was compared to microscopy for paired specimens from 107 patients in san francisco. microscopy was more than twice as sensitive as elisa: of 12 specimens positive by microscopy only 5 were also positive by elisa. the elisa did not detect a si ...19846095493
caerulomycin, an antifungal antibiotic with marked in vitro and in vivo activity against entamoeba histolytica.the anti-amoebic action of the bipyridyl antibiotic caerulomycin was assessed in vitro and in vivo using various strains of entamoeba histolytica from polyxenic, axenic and monoxenic cultures. minimum inhibition concentrations of caerulomycin (metronidazole) were 7.5 (5), 15.6(1.95) and 60 (2.5) micrograms/ml against polyxenic, axenic and monoxenic cultures of e. histolytica, respectively. the ed50 values ascertained in golden hamsters (extraintestinal amoebiasis) and rats (intestinal amoebiasis ...19846095553
amoebiasis in homosexual men. 19846096229
gastric amebiasis in a silvered leaf monkey. 19846096330
thiol groups on the surface of anaerobic parasitic protozoa.evidence is presented that giardia lamblia and entamoeba histolytica, phylogenetically unrelated aerotolerant anaerobes, have crucial thiol groups on or easily accessible to their external surface. both parasites were killed by three structurally unrelated thiol-blocking reagents which penetrate intact cells poorly or not at all. the parasites were protected from p-chloromercuribenzenesulfonic acid (10-100 microm) by cysteine or by reduced glutathione. killing was arrested with identical kinetic ...19846096710
[hepatic amebiasis with perforation of the pericardium]. 19846097284
[the size of entamoeba histolytica, entamoeba coli and giardia lamblia cysts]. 19846097375
effect of lowering the peripheral level of 5-hydroxytryptamine in host on the virulence of entamoeba histolytica. 19846097538
hepatic amebiasis. 19846098014
entamoeba histolytica: antibody responses and lymphoreticular changes in gerbils (meriones unguiculatus) in response to experimental liver abscess and amebic extract infection.antibody responses and histological changes in hepatic lymph nodes and spleen of gerbils (meriones unguiculatus) during the course of experimental hepatic amebiasis (5-60 days), or in those injected with extracts of entamoeba histolytica, are described. lymph node and spleen responses in infected animals paralleled the proliferation of the amebic liver abscess. however, spleen follicle responses were similar in animals that received low or high doses of the amebic extract and differed histologic ...19846098090
syntheses and biological activities of 1,4-disubstituted piperidines. 19846098241
[coproparasitological diagnosis by modified telemann's and burrows' methods]. 19846098283
antibiotics in the chemotherapy of amebiasis.amoebiasis is a widespread disease with high prevalence in tropical countries and environments with poor sanitation. the treatment of either intestinal and extra-intestinal localization of entamoeba histolytica or encephalic lesions sustained by acanthamoeba and naegleria has been the object of a wealth of research. antibiotics played a dominant role in this field. their antiamebic activity is due either to direct action on the protozoan and/or inhibition of symbiotic bacterial flora. presently ...19846099226
enzyme-linked immunosorbent assay in amoebiasis. 19846099332
[ultrastructural study of acid phosphatase localization in entamoeba histolytica trophozoites and the accompanying bacteria of the intestinal flora]. 19846099461
[histochemical study of entamoeba histolytica (schaudinn, 1903) obtained from the intestines of various hosts and histochemical and histological analysis of the intestines of infected animals]. 19846099635
antibody-dependent lymphocyte mediated cytotoxicity in amoebiasis. 19846099823
amoebicidal activity of the antifungal antibiotic sinefungin against entamoeba histolytica.the antifungal antibiotic sinefungin, a potent inhibitor of methyl transferases and a potential inhibitor of polyamine biosynthesis, exhibited marked cytolytic activity against entamoeba histolytica in vitro with respect to all the amoeba strains tested. strains of high and low virulence displayed equal sensitivity to the antibiotic. minimal amoebicidal concentration was 5 to 10 micrograms/ml.19846099924
a comparison of iha and elisa tests in the detection of antibodies to entamoeba histolytica.in this report we have evaluated the sensitivity and specificity of iha and elisa for the detection of anti-amebic antibodies in the serum samples of 147 patients with poor mental and hygienic status hospitalized in mental institutions. our data have demonstrated a positive agreement between elisa and iha on positive sera. the major drawback of iha is the occurrence of false negative results, 13/82 sera negative in iha (less than 1/128) are positive in the elisa. furthermore, the elisa is useful ...19846100069
immunochemical characterization of entamoeba histolytica plasma membrane's own and culture medium antigens. 19846100162
virulence of entamoeba histolytica trophozoites. effects of bacteria, microaerobic conditions, and metronidazole.the association of axenically grown trophozoites of entamoeba histolytica strains hk-9 or hm-1:imss with various types of gram-negative bacteria for relatively short periods markedly increased their virulence, as evidenced by their ability to destroy monolayers of tissue-cultured cells. interaction of trophozoites with bacteria that were heat inactivated, glutaraldehyde fixed, or disrupted by sonication, or bacteria treated with inhibitors of protein synthesis, did not augment amebic virulence. ...19846088660
the detection of serotonin: an additional approach in diagnosing amebiasis.the diagnosis of amebiasis is often difficult and time consuming. entamoeba histolytica has been shown to contain serotonin. this fact might be exploited in the clinical setting. assaying for serotonin may provide valuable diagnostic information.19846088956
genetic control of susceptibility of mice to infection with e. histolytica.genetic susceptibility to entamoeba histolytica infection in nine inbred strains and one outbred strain of mice was studied. the number of e. histolytica trophozoites in the ceca of the mice was examined 5 days after intracecal inoculation of axenic amoebae. c3h/hecr, balb/c, nzb/bin, b10.a, dba/2 and c57bl/6 were susceptible whereas a/j, ce, dba/1 and cd-1 mouse strains were relatively resistant. examination of f1 hybrid animals derived from susceptible b10.a and resistant a/j strains of mice s ...19846089075
increased macrophage migration inhibition factor production in hamsters sensitized by amoebic antigen and glucan.well defined cell-mediated immune responses were detectable following experimental immunization of hamsters with entamoeba histolytica antigen, using glucan as an adjuvant. peritoneal cells from amoeba antigen-glucan sensitized animals, upon incubation with specific antigen in vitro, were found to release into the supernatant a macrophage migration inhibition factor (mif). such supernatant fluids inhibited the migration of macrophages from non-sensitized hamsters. the production of mif was found ...19846089076
immuno-diagnosis of amoebiasis by antibody detection. 19846100369
entamoeba histolytica cyst passers: clinical features and outcome in untreated subjects.to examine whether entamoeba histolytica is the cause of a variety of intestinal disorders, as often implicated, a two-part study was conducted. in part 1, 184 patients attending a gastroenterology clinic and needing a sigmoidoscopy had a rectal biopsy specimen and a stool sample cultured for e histolytica. part of the biopsy specimen was examined histologically. in addition, serum was tested for antibody to e histolytica. 34 (18.7%) patients were culture positive. there was no correlation betwe ...19846146859
viruses and pathogenicity of entamoeba histolytica. 19846147594
viruses and the pathogenicity of entamoeba histolytica. 19846148562
cytopathogenicity of entamoeba histolytica.the lesions induced in man by entamoeba histolytica are characterized by massive tissue injury in the absence of major local signs of a host immune response. the amoeba damages surrounding cells preferentially by contact-mediated cytolysis. recently, a presumptive aetiological factor underlying this process has been identified. it is a protein, amoebapore, capable of spontaneous incorporation into host cell membranes. therein it induces high conductance ion-channels which rapidly collapse the ce ...19846151690
cell-mediated resistance induced by axenic amoebal rna--protein fraction.immunologically potent, rna protein fraction could be isolated from axenic entamoeba histolytica (nih: 200) using a phenol extraction method. immunization of animals with amoebal rna protein (eh rna) fraction evoked an intense cellular sensitization. in immunized animals the percent leucocyte migration inhibition was found to be 73.7 +/- 6.9. in control groups where the animals were treated with freund's complete adjuvant, yeast rna and amoebal rna treated with rnase, the percent leucocyte migra ...19846084696
metabolism of entamoeba histolytica schaudinn, 1903. 19846085216
entamoeba histolytica: inhibition in vitro by bithionol of respiratory activity and growth.endogenous and 2-propanol-supported respiration of intact trophozoites of of entamoeba histolytica (stain hm-1:imss) were inhibited by bithionol, an effective chemotherapeutic agent for some trematode and cestode infections in humans. dichlorophene and hexachlorophene also inhibited 2-propanol-supported respiration of the parasite. in contrast, ethanol formation by e. histolytica extract in the presence of n2 was scarcely inhibited by bithionol. the compound also inhibited in vitro growth of axe ...19846086381
effect of stress on the pathogenicity of entamoeba histolytica. 19846086513
effect of silica on resistance of mice to entamoeba histolytica infection.the role of macrophages in intestinal amoebiasis in mice has been investigated. the effect of injuring host macrophages on the course of infection was examined by using strains selected as being either genetically susceptible (c3h/hej, c57bl/6) or genetically resistant (a/j) to amoebiasis. mice were treated with an intravenous injection of silica particles 1 day before infection with 2.5 x 10(5) or 5 x 10(5) polyxenic trophozoites of entamoeba histolytica. the animals were killed at various time ...19846086527
comparison of the sensitivity of microscopy and culture in the laboratory diagnosis of intestinal protozoal infection.concentration of protozoal cysts from faeces by the formol-ether method and faecal culture on robinson's medium were studied to determine their diagnostic value among patients attending a sexually transmitted diseases clinic in edinburgh. of 450 men studied entamoeba histolytica and giardia lamblia were identified in the faeces of 10.7 and 5.1% respectively. thirty (81.1%) of 37 men with e histolytica and 11 (47.8%) of 23 men with g lamblia infections would not have been identified if formol-eth ...19846086726
[a copro-parasitological study on the wrestlers of the national team].this copro-parasitological research was made on the 18 wrestlers of the national team in the talas training camp in kayseri, the majority of them came to our laboratory with gastro-intestinal complaint such as nausea colicky pains anorexia and dyspepsia and other complaints such as exhaustion and fatigue and 16 of them were found infested with one or more than one parasites. as a result of our coprologic examination finding parasites and their infection rate were: entamoeba histolytica (50%), en ...19846087094
a weakly acidic protease has a powerful proteolytic activity in entamoeba histolytica.a thiol dependent protease from homogenates of the parasite entamoeba histolytica has been identified and partially purified by means of ammonium sulphate fractionation, gel filtration and isoelectric focusing. the protease, having a molecular mass of 21 +/- 2 kda as judged by gel chromatography, represents a highly potent proteolytic capacity. the protease shows maximal activity against azocasein around the slightly acidic ph of 4.4 at 37 degrees c, but is also active at ph 3.4 and 8.5. at opti ...19846087137
amebic appendicitis associated with hepatic abscess.we have described a case of primary amebic appendicitis with liver abscess, and we suggest that this diagnosis be considered in patients with appendicitis after returning from endemic areas. careful histologic preparation will establish the diagnosis and dictate appropriate therapy.19846087467
amebic prostatitis.a man with persistent prostatitis despite antibiotic therapy was found to have amebas compatible with entamoeba histolytica in his urine after prostatic massage. two indirect hemagglutination titers for e histolytica were negative. the patient had a prompt response to metronidazole therapy. prostatitis due to amebic infection should be considered in patients who do not respond symptomatically to standard antibiotic therapy.19846087468
enteric pathogenic protozoa in homosexual men from san francisco.the prevalence of enteric protozoa was studied in a survey conducted among 150 male homosexual patients in san francisco. all patients were from a private practice in internal medicine. each was asked to complete a questionnaire and to submit multiple stool specimens for examination. of this group, 47% were positive for one or more potentially pathogenic intestinal protozoa; entamoeba histolytica was found in 36%, entamoeba hartmanni in 35%, giardia lamblia in 5%, and dientamoeba fragilis in 1.3 ...19846087479
attempts at oral infection of rats and mice with trophozoites of entamoeba histolytica.groups of athymic rnu rats were found to be susceptible to saw 408 entamoeba histolytica introduced intra-intestinally. the same strain of rat could be infected orally with cultured trophozoites using a gastric secretion inhibitor, cimetidine, as a pretreatment. groups of lac:p and wistar rats similarly treated were also found to be susceptible although the parasite population growth was slow. a similar regimen attempted on susceptible mice was unsuccessful.19846087507
serological differentiation between past and present infection in hepatic amoebiasis.the indirect fluorescent antibody (ifa) test and the amoebic gel diffusion (agd) test were assessed as tools for differentiating between past and present infection in invasive amoebiasis; in the case of the ifa test the entamoeba histolytica-specific igg and igm were monitored. in a pilot study in which sera from 56 patients with suspected invasive amoebiasis were tested the igm was positive in 40% of confirmed intestinal and 83% of confirmed hepatic cases, the igg and agd were positive in all c ...19846087510
an outbreak of amoebic infection in a kibbutz population.the cause of a reported outbreak of abdominal cramps and diarrhoea in a kibbutz in the western negev, israel, was sought. over 25% of the kibbutz population was found to harbour entamoeba histolytica, a very high rate by israeli standards. the highest frequency of infection was in the nought to nine-year-old group. high frequencies were also observed among 30- to 49-year-old women. the sanitary and hygienic conditions in the kibbutz, checked at the time of the epidemic and five years later, were ...19846087511
a controlled study of endemic sporadic diarrhoea among adult residents of southern brazil.the aetiology of sporadic summer diarrhoea and enterotoxin (lt) antibody titres was studied among 43 adult patients in southern brazil who had an acute, untreated diarrhoeal illness and 43 age- and sex-matched controls from the same area. a potential pathogen was identified in 33 of 34 patients and in 17 of 43 controls (p less than 0.01). 10 shigella, one invasive escherichia coli, one salmonella and one entamoeba histolytica were identified in 17 patients with inflammatory diarrhoea (faecal pol ...19846087512
effect of thioacetamide on the susceptibility of rats to entamoeba histolytica infection. 19846087516
a fluorescent technique for demonstrating the chromatoid bodies and nuclei in cysts of entamoeba histolytica from faecal deposits. 19846200503
anti-ia reactivity in sera from subjects with entamoeba histolytica infection.sera from 15 patients with entamoeba histolytica infection were tested for anti t-cell antibodies by assessing cross-reacting specificities with the antigens defined by an anti-ia hybridoma antibody. t cells prepared by sheep erythrocyte rosetting were preincubated with the test sera and then with the anti-ia antibody. binding of the specific monoclonal antibody was assessed by rosetting with ox erythrocytes conjugated with goat anti-mouse igg. eight sera from amoebic patients were found to bloc ...19846200968
increased leucocyte histamine release by entamoeba histolytica antigen in patients with amoebic abscess of the liver.leucocytes (basophils) from non-atopic adult subjects living in an area highly endemic with entamoeba histolytica release histamine in a dose-dependent fashion upon in vitro exposure to an antigen of axenically grown e. histolytica (histolyticin). leucocytes of patients with acute amoebic liver abscess were significantly more sensitive to this antigen than leucocytes of control subjects, including patients that had recovered from amoebic liver abscess. by comparison concanavalin-a induced histam ...19846204266
the occurrence of beta-amylase in entamoeba histolytica.a beta-amylase like activity isolated from a cell homogenate of entamoeba histolytica, and separated from other glucohydrolytic activities by gel filtration and isoelectric focusing, hydrolyzed amylose, amylopectin and glycogen, yielding maltose as reaction product. from the non-reducing ends of the 4-nitrophenyl-alpha-d-glycosides of maltotriose, maltotetraose, maltopentaose and maltohexaose the enzyme removed maltose units. the ph optimum for hydrolyzing the poly- and oligosaccharides was ph 6 ...19846205266
immunogenicity of rna-protein fraction isolated from axenic entamoeba histolytica (nih:200).the immunogenicity of entamoeba histolytica rna (ehrna) was studied in guinea-pigs. animals immunized with a single dose of ehrna and freund's complete adjuvant (fca) showed a high level of cell-mediated immune response (cmir) as assessed by percent leucocyte migration inhibition (74.8 +/- 2.9) after 1 week of immunization. this response faded away gradually and by week 5 after immunization the percent leucocyte migration inhibition was 27.1 +/- 8.27, which is quite close to control values. the ...19846205649
endocytosis in entamoeba histolytica. evidence for a unique non-acidified compartment.our studies on endocytosis in entamoeba histolytica trophozoites suggest that there are two vacuolar compartments in this organism. the first compartment consists of large vacuoles (greater than 2 microns diameter). as measured by the fluid phase markers, fluorescein isothiocyanate (fitc)-dextran and horseradish peroxidase (hrp), this compartment is a rapid equilibrium with the external milieu and is constantly exchanging (1-2 h) its contents with the external medium. the contents of these vacuo ...19846206186
schistosoma mansoni, s. haematobium, and s. japonicum: identification of genus- and species-specific antigenic egg glycoproteins.immunoreactive egg glycoproteins of schistosoma mansoni, s. haematobium, and s. japonicum which are genus- and species-specific, or react with sera of patients infected with other parasites, have been identified. egg proteins were labeled with iodine-125, and the concanavalin a-binding glycoproteins were immunoprecipitated with sera of patients infected with one of four species of schistosoma or trichinella spiralis, taenia solium, echinococcus granulosus, entamoeba histolytica, or wuchereria ba ...19846209162
ketoconazole and other potent antimycotic azoles exhibit pronounced activity against trypanosoma cruzi, plasmodium berghei and entamoeba histolytica in vivo. 19846320547
proteinase activities of entamoeba histolytica cytotoxin.the proteinase activity of the low molecular weight cytotoxin of entamoeba histolytica was correlated with its cytotoxicity. gel-filtered amebal toxin (mol wt 10-30,000) proteinase activities could be assayed on azocasein at ph 6 or on hemoglobin at ph 4.5. proteinase activity was inhibited by serum fractions, thiol reagents, heavy metals, leupeptin, and antipain. the cytotoxic activity of gel-filtered amebal toxin was inhibited by serum fractions, leupeptin, and antipain. increased proteinase a ...19846327455
[intestinal amebiasis. a diagnosis with many faces]. 19846327559
protozoal enteric infections in homosexual men.the prevalence of enteric protozoal infections was examined in a group of 200 homosexual men. entamoeba histolytica (e. histolyt .) was found in 22.8%, apathogen amoeba in 54.5% and giardia lamblia (g. lamblia) in 9.1% of the participants. antibodies against e. histolyt . were significantly higher in subjects carrying e. histolyt . than in the group with apathogen amoeba and in uninfected participants. clinical symptoms were not associated with protozoal infection. the analysis of a number of va ...19846328099
concomitant and secondary bacterial infection of the pus in hepatic amoebiasis.in a series of 48 consecutive patients with clinically diagnosed uncomplicated amoebic liver abscess (ala), the pus obtained by closed drainage of the abscess was bacteriologically sterile. secondary bacterial infection of the ala as a direct result of the aspiration procedure occurred in only 1 patient, the causative organism being escherichia coli. entamoeba histolytica was identified in a total of 39 cases (81%) by means of direct microscopy of the pus as well as culture methods.19846328682
immune phenomena in amebiasis. 19846326234
lectin-induced agglutination of trophozoites of different species and strains of entamoeba.in vitro agglutinability of trophozoites of three entamoeba histolytica strains, cultivated under axenic conditions in the presence of concanavalin a (con a), was shown to be related to the degree of their pathogenicity for experimental animals and of the concentration of con a. seven strains of e. invadens tested also agglutinated in the presence of con a, and the degree of agglutination was proportional to the concentration of the lectin. three strains of e. histolytica-like "laredo-type" ameb ...19846326409
kinetics of peripheral blood t cell numbers and functions in patients with burns.cell-mediated immunity (cmi) was assessed in 58 patients with burns involving 10% to 40% of the total body surface area (tbsa), and in 16 normal healthy individuals, by using two parameters, the total t-cell counts in peripheral circulation and t-cell function as indicated by the leukocyte migration inhibition (lmi) test. three recall antigens from candida albicans, s. typhi, and e. histolytica were used to elicit the lmi. t-cell function was found to be significantly depressed in burns involvin ...19846323729
[an unnecessarily long course of a disease]. 19846324008
influence of geographical factors in the distribution of pathogenic zymodemes of entamoeba histolytica: identification of zymodeme xiv in india.stocks of entamoeba histolytica isolated and maintained in a variety of culture media, both axenic and polyxenic, were compared with each other and with strains previously characterized. the present stocks were isolated from subjects living in various cities in india. using the enzyme patterns of: e c 5319 glucose phosphate isomerase (gpi); e c 11140 l-malate: nadp+ oxidoreductase (oxaloacetate decarboxylating) (me): e c 2751 phosphoglucomutase (pgm); and e c 2711 hexakinase (hk), developed afte ...19846324421
serum antibodies to giardia intestinalis detected by immunofluorescence using trophozoites as antigen.anti-giardia antibodies were studied in 88 sera of patients with symptomatic and asymptomatic giardiasis by using the indirect immunofluorescent test and giardia intestinalis trophozoites from an axenic culture as antigen. the sera of 40 healthy subjects were used as a control group and of 28 patients with other intestinal parasites (14 with sarcocystis sp. and 14 with symptomatic entamoeba histolytica infection) have been investigated for determination of test specificity. 82% of sera from pati ...19846324429
[entamoeba histolytica: ii. effect of humoral immune mechanisms on cytotoxic activity].the time dependency of the cytotoxic action of e. histolytica against tissue culture cells of mammalian origin was assessed in a 51cr-release assay. obviously, the amebae-dependent cytotoxic activity within the first 30-60 min of the assay against k562 (an erythroleukemic cell line) and mh1c1 (a rat hepatoma cell line) correlated with the pathogenicity of the respective amebae in vivo as measured by the hamster liver infectivity test. the in vivo apathogenic strain of e. histolytica (hk9) exhibi ...19846324430
microvolume, kinetic-dependent enzyme-linked immunosorbent assay for amoeba antibodies.we describe a microvolume enzyme-linked immunosorbent assay based on enzyme rate kinetics. antigens from entamoeba histolytica were adsorbed in wells of disposable polystyrene strips containing 12 flat-bottom wells. after exposure to the serum of a patient and peroxidase-labeled anti-human immunoglobulin g, the rate of color change in specific substrate was determined by eight sequential readings of individual wells over a 2-min period with a microcomputer-controlled model mr-600 automated plate ...19846321547
entamoeba histolytica: a eukaryote without glutathione metabolism.entamoeba histolytica was found to grow normally without producing glutathione and the main enzymes of glutathione metabolism, indicating that glutathione is not essential for many eukaryotic processes. this parasitic amoeba is an unusual eukaryote whose special features may help define the crucial functions of glutathione in those eukaryotes that do use it. since entamoeba histolytica lacks mitochondria and the usual aerobic respiratory pathways, the finding that it grows without glutathione an ...19846322306
amoebiasis in homosexual men.the clinical, histopathological, and serological features of 35 homosexual men with infection with entamoeba histolytica were studied and compared with a group of 35 non-infected homosexual men. each isolate was of zymodeme type i. although there was no significant difference in the numbers of infected and non-infected men with gastrointestinal symptoms (48.6% and 28.6% respectively), the mean duration of symptoms was greater in with amoebiasis (p less than 0.05). the histology of the rectal muc ...19846323279
a long-term follow up study of amoebiasis treated with metronidazole.metronidazole, in a dose of 40 mg/kg body weight in 3 divided doses for 10 days was used to treat 194 patients infected with entamoeba histolytica. when 166 subjects were checked 1 month after treatment, with 3 stool samples taken on different days, 20 failures were found giving a parasitic cure rate of 88%. a lower-than-expected failure rate was noted in children, immigrants and asymptomatic subjects. no more failures were found in 78 patients who went through an extended follow-up, with examin ...19846429847
hemolytic-uremic syndrome and entamoeba histolytica infection. 19846473145
immunodiagnosis of giardiasis by elisa and studies on cross-reactivity between the anti-giardia lamblia antibodies and some heterologous parasitic antigens and fractions.specific antibodies against giardia lamblia in 92 sera from asymptomatic carriers were investigated by elisa. using cyst antigen, antibodies were detected in 91 of the sera, by means of alkaline phosphatase-labelled antispecies immunoglobulin. the e/405nm values of the positive sera ranged between 0.350 and 1.700, whereas in the 78 control sera they were 0.270 +/- 0.220. cross-reactivity between the anti-g. lamblia antibodies and 18 heterologous parasitic antigens and fractions was also studied. ...19846486935
[report on intestinal parasitosis in adults in an urban population of the central african republic].the authors point out the frequency of intestinal parasitism in bangui, biggest town and capital of the car. out of 3,352 samples, 1,570 were found positive. the main parasites found were: ankylostoma duodenale, 26,7%; schistosoma mansoni, 20,8%; entamoeba histolytica, 18,2%. the authors underline the polyparasitism. they also emphazide the differences with another study carried out in algiers and surrounding areas.19846488424
histological evolution of caecal lesions in experimental amoebiasis.histopathological studies in guinea-pigs infected intracaecally with entamoeba histolytica were carried out longitudinally on days 3, 7, 11, 15, 20 and 35 after infection. three characteristic modes of development of caecal lesions were observed. these in order of their prevalence were the classical inflammatory cellular infiltration, lymphoid hypertrophy and necrotizing colitis. occasional amoeboma formation was observed in progesterone-treated animals. this is the first time that histological ...19846506152
intestinal parasites in pediatric patients with diarrhoeal diseases in bangkok.stool examinations of 147 pediatric patients with diarrhoeal disease were carried out at siriraj hospital, bangkok by using the direct-smear technique. stool of 27 patients (18.4%) were positive for intestinal parasites. children under one year of age were free of intestinal helminths and protozoa. parasites were equally prevalent in males and females and without any age group predilection. the prevalence of intestinal parasites were entamoeba histolytica 6.8%, giardia lamblia 6.1%, others were ...19846523173
[epidemiologic survey of intestinal parasitoses in peruvian amazonia].an epidemiological survey was carried out in 4 native villages from tribe cashibo, in amazonia (peru). among 165 stools examination, 92% were positives for helminthic (hookworms: 72%, roundworms and whipworms: 55% each, strongyloides: 5%) and protozoan infections (entamoeba coli and endolimax nana: 50% each, entamoeba histolytica 24%, giardia intestinalis 17%). balantidium coli is not seldom (6%). polyparasitism, very frequent, is reported with a maximum of 10 species. the most important village ...19846525727
[hepatic amebiasis: study of 152 cases].the purpose of this study was to describe and analyse retrospectively the clinical, serological, anatomical and evolutive features of 152 cases of hepatic amebiasis in young adults, treated and followed up in france from 1969 to 1983. the disease was revealed 3 times out of 4 by tender hepatomegaly with fever, but only in 6 cases by complications. serological tests (immunofluorescence or hemagglutination) were always positive for amebiasis, whereas entamoeba histolytica was absent from stools in ...19846526240
serological studies of entamoeba histolytica infection in north indian population. 19846532965
parasitic, bacterial, and viral enteric pathogens associated with diarrhea in the central african republic.a total of 1,197 diarrheic children less than 15 years old were investigated for parasitic, bacterial, and viral enteropathogens from march 1981 through february 1982 in the central african republic. one or more pathogens were identified from 49.4% of the patients. rotavirus was the most frequently identified pathogen among children less than 18 months old. enteropathogenic escherichia coli was the second most frequently isolated pathogen (12.1%) in children less than 2 years of age. campylobact ...19846330161
inhibition of growth of giardia lamblia by difluoromethylornithine, a specific inhibitor of polyamine biosynthesis.difluoromethylornithine (dfmo) is a specific and irreversible inhibitor of ornithine decarboxylase, an enzyme which catalyzes the first step in the biosynthetic pathway of the polyamines. we tested the effect of dfmo on the growth of giardia lamblia, entamoeba histolytica, and trichomonas vaginalis. growth of g. lamblia was inhibited by dfmo at concentrations of greater than or equal to 1.25 mm. culture doubling time increased with increasing dfmo concentration. growth inhibition was reversed if ...19846330350
intestinal parasitic infection and other sexually transmitted diseases in asymptomatic homosexual men.76/133 (57%) asymptomatic homosexual men harboured intestinal parasites. of these, 40 had entamoeba histolytica or giardia lamblia, or both. in a control group of heterosexual men, no pathogenic protozoa were found. stool specimen cultures for salmonella, shigella, and campylobacter were negative. 7% of the homosexual men were infected with neisseria gonorrhoeae in the pharynx or rectum, or both, and 5% with chlamydia trachomatis in the urethra or rectum. serological evidence of syphilis was det ...19846330881
seroepidemiology of amoebiasis using finger prick blood samples. 19846330943
[parasite antigens--problems of immunodiagnosis]. 19846331013
pathogenic and free-living protozoa cultured from the nasopharyngeal and oral regions of dental patients.protozoa of nose, mouth, and pharynx of 30 randomly chosen female caries patients at an odontological clinic of the national autonomous university of mexico, were surveyed by culture from swabs. culture tubes of swabs from each patient were observed every other day during 5 weeks. pathogenic protozoa found included entamoeba histolytica schaudinn, 1903; naegleria fowleri carter, 1970; acanthamoeba castelanii douglas, 1930; acanthamoeba culbertsoni singh & das, 1970; and balantidium coli (malmste ...19846370674
cellular bases of experimental amebic liver abscess formation.the complete sequence of morphologic events during amebic liver abscess formation in the hamster has been studied, from the lodgement of amebas in the hepatic sinusoids to the development of extensive liver necrosis. following intraportal inoculation of live amebas, the early stages of the lesion (from 1 to 12 hours) were characterized by acute cellular infiltration composed of an increasingly large number of polymorphonuclear leukocytes, which surrounded centrally located trophozoites. histiocy ...19846385728
the prevalence of gastro-intestinal parasites and bacteria in the game scouts at the kainji lake national park of nigeria.the faeces of twenty scouts working at borgu games were examined for the presence of parasites and bacteria. cysts of entamoeba histolytica, giardia lamblia and trichomonas hominis were found. helminth ova encountered included those of ancylostoma duodenale, ascaris lumbricoides, stronglyloides stercoralis and trichuris trichiura. escherichia coli and shigella flexneri were isolated from one faecal sample each. in all, parasites were found in the faeces of only 9 scouts. the epidemiological sign ...19846389403
[hepatic amebiasis in french polynesia. 16 cases in 2 years].asymptomatic amebiasis in known for long ago in french polynesia; the presence of entamoeba histolytica cyst carriers have been shown by several parasitological investigations. but, until now, no hepatic amebiasis case has been reported in this geographical area. an homogeneous series of 16 cases of hepatic amebiasis is reported here. the diagnosis was made by hepatic ultrasonography and confirmed by immunology (indirect immunofluorescent tests gave always a positive reaction for a dilution of 1 ...19846390078
schistosoma mansoni, s. haematobium, and s. japonicum: identification of genus-, species-, and gender-specific antigenic worm glycoproteins.immunoreactive glycoproteins of schistosoma mansoni, s. haematobium, and s. japonicum adult worms which are genus-, species-, and gender-specific, or react with sera of patients infected with other parasites, have been identified. schistosomes were metabolically labeled with [35s]methionine and the concanavalin-a binding glycoproteins that were immunoprecipitated with sera of patients infected with one of three species of schistosoma or trichinella spiralis, taenia solium, echinococcus granulosu ...19846692879
the mongolian gerbil (meriones unguiculatus) as an experimental host for entamoeba histolytica.experimentally induced cecal and hepatic amebiasis was studied in the mongolian gerbil (meriones unguiculatus). axenically cultivated entamoeba histolytica trophozoites were inoculated into the cecum or the liver after laparotomy. cecal infections were characterized by intense inflammation and distortion of the tissue. the intensity of the infection, measured by a standard method of scoring, was consistently high from 10 to 30 days postinoculation (p.i.). in tissue sections, disruption of the in ...19846696183
nitroimidazoles in the treatment of trichomoniasis, giardiasis, and amebiasis.nitroimidazoles have been extensively evaluated in the treatment of trichomoniasis, giardiasis, liver and intestinal amebiasis. the most widely used are metronidazole, tinidazole, ornidazole, and secnidazole. tinidazole, ornidazole, and secnidazole have a much longer half-life than metronidazole, allowing single-dose or once daily administration. nitro-5-imidazoles remain extremely effective drugs for treating protozoans, trichomonas vaginalis, entamoeba histolytica, and giardia intestinalis. al ...19846698665
amebiasis presenting as multiple apple core lesions.an unusual manifestation of amebiasis is presented to demonstrate its ability to masquerade as carcinoma or a variety of colitides. the importance of actively considering entamoeba histolytica-induced disease in a differential diagnosis of bloody diarrhea is emphasized.19846702807
intestinal protozoa in homosexual men of the san francisco bay area: prevalence and correlates of infection.to compare the prevalence of intestinal parasitic infection in male homosexuals selected from other sources with the levels previously reported from clinic-derived populations, various homosexual groups of the san francisco bay area were checked by means of stool examination and questionnaires for prevalence of intestinal parasites and for related sexual behavioral patterns during a 3-year period. the prevalence of entamoeba histolytica (28.6%) was similar to that reported in other studies, wher ...19846711741
parasites found in the mouths of inhabitants of three villages of south kalimantan (borneo), indonesia.infection rates of parasites in the oral cavity were compared for two study areas in south kalimantan, indonesia. penguiran and sungai baru, two adjacent villages, were considered a single study area and were inhabited by 151 individuals ranging from 2 to 70 years. tanah intan, the second study area, had a population of 265 persons who also ranged from 2 to 70 years. the standard of living was low in all the villages (average of 450 u.s. dollars per family per year). teeth and gums were examined ...19846729983
[amebic liver abscess--diagnostic and therapeutic advances].liver abscess is the most frequent complication of intestinal disease caused by entamoeba histolytica. noninvasive procedures are nowaday most important in establishing the diagnosis: sonography, scintigraphy and computer tomography (ct). sonography is rather precise and should be used in the first place. recently it became possible to demonstrate specific antigens to amoeba in the pus of the abscess or in liver biopsy material; this procedure has been proven to be dependable and fast in establi ...19846738288
a bifunctional thymidylate synthetase-dihydrofolate reductase in protozoa.thymidylate synthetase and dihydrofolate reductase exist as a bifunctional protein in a number of species of protozoa which span diverse groups of the subkingdom. the enzymes copurify upon gel filtration and on affinity chromatography columns specific for dihydrofolate reductase. the bifunctional protein has been found in species of crithidia, leishmania, trypanosoma, plasmodium, eimeria, tetrahymena and euglena. for reasons unknown, neither enzyme could be detected in entamoeba histolytica or e ...19846749182
amoebic liver abscess: an auckland experience.globally, liver abscess caused by entamoeba histolytica is a common condition. in new zealand, however, it occurs infrequently. eight cases were diagnosed in auckland between 1972 and 1982, each patient having travelled through an endemic area within two years of presentation. management often was not ideal, with both delays in diagnosis and inappropriate treatment. there is a need for increased awareness of possible amoebic abscesses in those who have been in endemic areas because with prompt u ...19846583578
detection of amoebic antibodies using fractionated antigen in tia and iha tests.thin layer immunoassay (tia) test was used to detect anti-amoebic antibodies in the sera obtained from immunized guinea pigs. a few human sera samples were also used for antibody detection. the tia and iha results with whole amoeba antigen and fractionated antigen preparations were compared. both test procedures gave better results with fractionated antigen. the results in tia were better than in iha tests. thin layer immunoassay technique can be conveniently used as an alternative to iha test f ...19846328711
murine amebiasis: the role of the macrophage in host defense.the congenitally athymic nude (nu/nu) mouse was studied as a model for amebic liver disease, using entamoeba histolytica. despite intrahepatic inoculation of massive numbers of amebae, we could not produce sustained infections in nu/nu mice. we also failed to induce hepatic amebiasis in thymus-intact nu/+ and +/+ littermates, or in nu/+ mice pretreated with rabbit antimouse thymocyte globulin. humoral response was measured in both the nu/nu and nu/+ mice. nu/+ but not nu/nu animals developed a s ...19846329011
entamoeba histolytica: virulence enhancement of isoenzyme-stable parasites.pathogenic entamoeba histolytica isolated from patients with clinical amoebiasis can be differentiated from nonpathogenic e. histolytica obtained from asymptomatic carriers on the basis of the electrophoretic pattern of their isoenzymes. virulence of different strains of axenically grown trophozoites of entamoeba histolytica, as determined by various laboratory tests, such as damage to tissue culture monolayers, or their ability to cause an hepatic abscess in a hamster, are known to vary conside ...19846325226
relationship of cytotoxins of axenically cultivated entamoeba histolytica to virulence.clarified homogenates prepared from different strains of axenically reared entamoeba histolytica were assayed for acid and neutral proteinase activity, cytotoxicity, and protein content. the homogenates of the more virulent strains contained more acid or neutral proteinase and more toxin units per 10(6) trophozoites. toxicity and neutral proteinase activities had a similar pattern of distribution. the cytotoxicity of the gel-filtered proteins of the studied strains followed the same rank order a ...19846325286
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