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steam pretreatment of saccharum officinarum l. bagasse by adding of impregnating agents for advanced bioethanol production.the main byproduct of the sugarcane industry, saccharum officinarum l. bagasse (sugarcane bagasse, scb), is widely used as lignocellulose biomass for bio-ethanol (etoh) production. in this research study, scb was pretreated by steam explosion (se) method using two different impregnating agents: sulfur dioxide (sd) and hydrogen peroxide (hp). as matter of fact, the use of impregnating agents improves the performance of se method, increasing the concentrations of fermentable sugars after enzymatic ...201626314609
soil quality indexing strategies for evaluating sugarcane expansion in brazil.increasing demand for biofuel has intensified land-use change (luc) for sugarcane (saccharum officinarum) expansion in brazil. assessments of soil quality (sq) response to this luc are essential for quantifying and monitoring sustainability of sugarcane production over time. since there is not a universal methodology for assessing sq, we conducted a field-study at three sites within the largest sugarcane-producing region of brazil to develop a sq index (sqi). the most common luc scenario (i.e., ...201626938642
co-expression network analysis reveals transcription factors associated to cell wall biosynthesis in sugarcane.sugarcane is a hybrid of saccharum officinarum and saccharum spontaneum, with minor contributions from other species in saccharum and other genera. understanding the molecular basis of cell wall metabolism in sugarcane may allow for rational changes in fiber quality and content when designing new energy crops. this work describes a comparative expression profiling of sugarcane ancestral genotypes: s. officinarum, s. spontaneum and s. robustum and a commercial hybrid: rb867515, linking gene expre ...201626820137
spatial distribution and dynamics of sucrose metabolising enzymes in radiation induced mutants of sugarcane.sucrose metabolism in various source and sink organs of developing sugarcane (saccharum officinarum l.) plant is accompanied with continuous synthesis and cleavage. in this regard, the involvement of four major enzymes viz. sucrose synthase (ss), sucrose phosphate synthase (sps), soluble acid (sai) and neutral invertases (ni) is considered crucial. in this study, we have analysed in vivo enzymatic activity of 12th month old field grown radiation-induced sugarcane mutants identified for sucrose a ...201626795733
protective effect of saccharum officinarum l. (sugar cane) juice on isoniazid induced hepatotoxicity in male albino mice.isoniazid (inh) is the drug of choice for treatment of tuberculosis (tb) and it is a well-known-cause of acute clinical liver injury which can be severe and sometimes fatal. the study was designed to investigate the effects of saccharum officinarum l. juice on oxidative liver injury due to inh in mice.201626411113
prionic acid: an effective sex attractant for an important pest of sugarcane, dorysthenes granulosus (coleoptera: cerambycidae: prioninae).male dorysthenes granulosus (thomson, 1860) (coleoptera: cerambycidae: prioninae) were caught in traps baited with racemic 3,5-dimethyldodecanoic acid (prionic acid) during field screening trials in china that tested known cerambycid pheromones. this species is an important pest of sugarcane (saccharum officinarum l.). in follow-up dose-response trials, plastic sachets loaded with 1 or 0.1 mg of prionic acid were equally attractive to male beetles, whereas lower doses were no better than control ...201626362990
morphogenic regulators baby boom and wuschel improve monocot transformation.while transformation of the major monocot crops is currently possible, the process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond the reach of most academic laboratories. here, we report a transformation approach involving overexpression of the maize (zea mays) baby boom (bbm) and maize wuschel2 (wus2) genes, which produced high transformation frequencies in numerous previously non-transformable maize i ...201627600536
phytochemical profile of sugarcane and its potential health aspects.sugarcane (saccharum officinarum linn.) is an important perennial grass of poaceae family, indigenous to tropical south asia and southeast asia. it is cultivated worldwide due to the economical and medicinal value of its high yielding products. sugarcane juice is well known as a raw material for the production of refined sugar and its wax is considered as a potential substitute for the expensive carnauba wax, which is of cosmetic and pharmaceutical interest. refined sugar is the primary product ...201726009693
analysis of biomechanical parameters in colonic anastomosis.the use of measures in colonic anastomoses to prevent dehiscences is of great medical interest. sugarcane molasses, which has adequate tolerability and compatibility in vivo, has not yet been tested for this purpose.201727438033
an automatic on-line 2,2-diphenyl-1-picrylhydrazyl-high performance liquid chromatography method for high-throughput screening of antioxidants from natural products.many natural products are rich in antioxidants which play an important role in preventing or postponing a variety of diseases, such as cardiovascular and inflammatory disease, diabetes as well as breast cancer. in this paper, an automatic on-line 2,2-diphenyl-1-picrylhydrazyl-high performance liquid chromatography (dpph-hplc) method was established for antioxidants screening with nine standards including organic acids (4-hydroxyphenylacetic acid, p-coumaric acid, ferulic acid, and benzoic acid), ...201728939230
partially consolidated bioprocessing of mixed lignocellulosic feedstocks for ethanol production.rapid urbanization and industrialization have accelerated the energy demand which cannot be met by decreasing fossil fuels thereby substantiate the need for lignocellulosic ethanol. the present study is one such attempt towards bioethanol production in an eco-friendly manner using enzymes in which a mixture of lignocellulosic biomass namely, ricinus communis, saccharum officinarum (tops) and saccharum spontaneum were taken as a substrate. the mixed biomass was processed through partially consoli ...201728898853
production of a generic microbial feedstock for lignocellulose biorefineries through sequential bioprocessing.lignocellulosic materials, mostly from agricultural and forestry residues, provide a potential renewable resource for sustainable biorefineries. reducing sugars can be produced only after a pre-treatment stage, which normally involves chemicals but can be biological. in this case, two steps are usually necessary: solid-state cultivation of fungi for deconstruction, followed by enzymatic hydrolysis using cellulolytic enzymes. in this research, the utilisation of solid-state bioprocessing using th ...201728013134
the relation between xyr1 overexpression in trichoderma harzianum and sugarcane bagasse saccharification performance.this work investigates the influence of the positive regulator xyr1 of trichoderma harzianum on the production of cellulolytic enzymes, using sugarcane bagasse as carbon source. constitutive expression of xyr1 was achieved under the control of the strong trichoderma reesei pki1 promoter. five clones with xyr1 overexpression achieved higher xyr1 expression and greater enzymatic productivity when cultivated under submerged fermentation, hence validating the genetic construction for t. harzianum. c ...201728192217
addition of surfactants and non-hydrolytic proteins and their influence on enzymatic hydrolysis of pretreated sugarcane bagasse.poly(ethylene glycol) (peg 4000) and bovine serum albumin (bsa) were investigated with the purpose of evaluating their influence on enzymatic hydrolysis of sugarcane bagasse. effects of these supplements were assayed for different enzymatic cocktails (trichoderma harzianum and penicillium funiculosum) that acted on lignocellulosic material submitted to different pretreatment methods with varying solid (25 and 100 g/l) and protein (7.5 and 20 mg/g cellulose) loadings. the highest levels of glucos ...201727631122
expression analysis of hairpin rna carrying sugarcane mosaic virus (scmv) derived sequences and transgenic resistance development in a model rice plant.developing transgenic resistance in monocotyledonous crops against pathogens remains a challenging area of research. sugarcane mosaic virus (scmv) is a serious pathogen of many monocotyledonous crops including sugarcane. the objective of present study was to analyze transgenic expression of hairpin rna (hprna), targeting simultaneously cp (coat protein) and hc-pro (helper component-proteinase) genes of scmv, in a model rice plant. conserved nucleotide sequences, exclusive for dag (aspartic acid- ...201728255554
field performance of transgenic sugarcane lines resistant to sugarcane mosaic virus.sugarcane mosaic disease is mainly caused by the sugarcane mosaic virus (scmv), which can significantly reduce stalk yield and sucrose content of sugarcane in the field. coat protein mediated protection (cpmp) is an effective strategy to improve virus resistance. a 2-year field study was conducted to compare five independent transgenic sugarcane lines carrying the scmv-cp gene (i.e., b2, b36, b38, b48, and b51) with the wild-type parental clone badila (wt). agronomic performance, resistance to s ...201728228765
an atypical thioredoxin imparts early resistance to sugarcane mosaic virus in maize.sugarcane mosaic virus (scmv) causes substantial losses of grain yield and forage biomass in susceptible maize worldwide. a major quantitative trait locus, scmv1, has been identified to impart strong resistance to scmv at the early infection stage. here, we demonstrate that zmtrxh, encoding an atypical h-type thioredoxin, is the causal gene at scmv1, and that its transcript abundance correlated strongly with maize resistance to scmv. zmtrxh alleles, whether they are resistant or susceptible, sha ...201728216424
roles of reversible and irreversible aggregation in sugar processing.colloids (1-1000-nm particles) in sugarcane/beet juice originate from non-sucrose impurities (polyphenolic colorants, residual soil, polysaccharides) of the plant materials; additional colloids form during the high temperature processing. colloids are reactive toward aggregation, sorption, desorption, and redox/hydrolysis/thermal transformation reactions. both derjaguin-landau-verwey-overbeek (dlvo; van der waals and electrostatic forces) and non-dlvo (involving hydrophilic colloids) interaction ...201726065543
toxicological effects of the waste of the sugarcane industry, used as agricultural fertilizer, on the test system allium cepa.sugarcane is cultivated in tropical countries for sugar and ethanol production. in brazil, this culture is among the most profitable with a production of 658.7 million tons/harvest. sugarcane filter cake (scfc) is a waste rich in organic matter and micronutrients, but also contains toxic metals. as it has been used as fertilizer and there is not enough knowledge about its environmental impacts, this work assessed the genotoxicogenetic effects of raw scfc and associations with soil and sugarcane ...201728104478
adsorption of pharmaceuticals in water through lignocellulosic fibers synergism.the contamination of water from disposal of drugs is an emerging problem due to their consequences on trophic webs. this study evaluated the ability of sugarcane and coconut fiber to reduce water toxicity contaminated by pharmaceuticals. the toxicity of solutions containing pharmaceuticals was studied by bioassay using allium cepa, before and after filtration of contaminated water. the coconut and sugarcane fiber have not been satisfactory in reducing toxicity when tested separately. despite no ...201728002767
insect and plant-derived mirnas in greenbug (schizaphis graminum) and yellow sugarcane aphid (sipha flava) revealed by deep sequencing.schizaphis graminum (green bug; gb) and sipha flava (yellow sugarcane aphid; ysa) are two cereal aphid species with broad host ranges capable of establishing on sorghum (sorghum bicolor) and several switchgrass (panicum virgatum) cultivars. switchgrass and sorghum are staple renewable bioenergy crops that are vulnerable to damage by aphids, therefore, identifying novel targets to control aphids has the potential to drastically improve yields and reduce losses in these bioenergy crops. despite th ...201727838454
the assessment and the farmers' perceived ranking of feed resources and coping strategies with feed scarcity in smallholder dairy farming in selected district towns of jimma zone, ethiopia.inadequate quantity and quality of feed resources are major constraints limiting milk production and reproductive performance of dairy cattle in ethiopia. the aim of this study was to assess dairy cattle feed resources, feeding practices, the farmers' perceived ranking of feed resources, causes of feed shortage, and coping strategies to feed scarcity in smallholder dairy system in selected district towns of jimma zone, ethiopia. data were obtained by interviewing 52 randomly selected smallholder ...201728389982
energy and protein requirements for growth of holstein × gyr heifers.there is little information regarding the nutritional requirements for dairy heifers, leading the majority of nutrient requirement systems to consider dairy heifers to be similar to beef heifers. therefore, we evaluated the muscle protein metabolism and physical and chemical body composition of growing holstein × gyr heifers and estimated the energy and protein requirements. we performed a comparative slaughter experiment with 20 holstein × gyr heifers at an initial body weight of 218 ± 36.5 kg ...201728299852
growth and expression of relevant metabolic genes of clostridium thermocellum cultured on lignocellulosic residues.the plant cell wall is a source of fermentable sugars in second-generation bioethanol production. however, cellulosic biomass hydrolysis remains an obstacle to bioethanol production in an efficient and low-cost process. clostridium thermocellum has been studied as a model organism able to produce enzymatic blends that efficiently degrade lignocellulosic biomass, and also as a fermentative microorganism in a consolidated process for the conversion of lignocellulose to bioethanol. in this study, a ...201728181082
high-pressure carbon dioxide/water pre-treatment of sugarcane bagasse and elephant grass: assessment of the effect of biomass composition on process efficiency.the performance of two lignocellulosic biomasses was studied in high-pressure carbon dioxide/water pre-treatment. sugarcane bagasse and elephant grass were used to produce c5-sugars from hemicellulose and, simultaneously, to promote cellulose digestibility for enzymatic saccharification. different pre-treatment conditions, with combined severity factor ranging from -1.17 to -0.04, were evaluated and maximal total xylan to xylose yields of 59.2wt.% (34.4wt.% xylooligomers) and 46.4wt.% (34.9wt.% ...201727955864
decomposition rate and enzymatic activity of composted municipal waste and poultry manure in the soil in a biofuel crops field.biofuel crops are gaining importance because of the need to replace non-renewable sources. also, due to the increasing amounts of wastes generated, there is the need to recycle them to the soil, both to fertilize crops and to improve soil physical properties through organic matter increase and microbiological changes in the rhizosphere. we therefore studied the influence of six biofuel crops (elephant grass, giant cane, sugarcane, blue gum, black cottonwood, willow) on the decomposition rate and ...201727620351
efficacy of insect growth regulators and diamide insecticides for control of stem borers (lepidoptera: crambidae) in sugarcane.field experiments assessed control of the stem borers, diatraea saccharalis (f.) and eoreuma loftini (dyar), in sugarcane (saccharum spp.) with selected insect growth regulators and diamide insecticides. tebufenozide, novaluron, chlorantraniliprole, and flubendiamide were evaluated for d. saccharalis control in four field experiments in louisiana between 2009 and 2014. aerial application of the same insecticides was evaluated for control of e. loftini in commercial sugarcane fields in southern t ...201728334153
ssr-enriched genetic linkage maps of bermudagrass (cynodon dactylon × transvaalensis), and their comparison with allied plant genomes.we report ssr-enriched genetic maps of bermudagrass that: (1) reveal partial residual polysomic inheritance in the tetraploid species, and (2) provide insights into the evolution of chloridoid genomes. this study describes genetic linkage maps of two bermudagrass species, cynodon dactylon (t89) and cynodon transvaalensis (t574), that integrate heterologous microsatellite markers from sugarcane into frameworks built with single-dose restriction fragments (sdrfs). a maximum likelihood approach was ...201728168408
estimating 20-year land use change and derived co2 emissions associated to crops, pasture and forestry in brazil and each of its 27 states.land use change (luc) in brazil has important implications on global climate change, ecosystem services and biodiversity, and agricultural expansion plays a critical role in this process. concerns over these issues have led to the need for estimating the magnitude and impacts associated to that, which are increasingly reported in the environmental assessment of products. currently, there is an extensive debate on which methods are more appropriate for estimating luc and related emissions and reg ...201728370797
techno-economic analysis and climate change impacts of sugarcane biorefineries considering different time horizons.ethanol production from lignocellulosic feedstocks (also known as 2nd generation or 2g ethanol process) presents a great potential for reducing both ethanol production costs and climate change impacts since agricultural residues and dedicated energy crops are used as feedstock. this study aimed at the quantification of the economic and environmental impacts considering the current and future scenarios of sugarcane biorefineries taking into account not only the improvements of the industrial proc ...201728293288
effects of air pollution caused by sugarcane burning in western são paulo on the cardiovascular system.to evaluate the effects of acute exposure to air pollutants (no2 and pm10) on hospitalization of adults and older people with cardiovascular diseases in western são paulo.201728273230
defense-related proteins involved in sugarcane responses to biotic stress.sugarcane is one of the most important agricultural crops in the world. however, pathogen infection and herbivore attack cause constant losses in yield. plants respond to pathogen infection by inducing the expression of several protein types, such as glucanases, chitinases, thaumatins, peptidase inhibitors, defensins, catalases and glycoproteins. proteins induced by pathogenesis are directly or indirectly involved in plant defense, leading to pathogen death or inducing other plant defense respon ...201728222203
unraveling the influence of the cod/sulfate ratio on organic matter removal and methane production from the biodigestion of sugarcane vinasse.throughout the sugarcane harvest, it is common for sulfate to accumulate in the vinasse of sugar and ethanol plants. however, little is known regarding the influence of sulfate on the anaerobic digestion (ad) of vinasse, which may lead to severe performance losses. this study assessed the influence of various cod/sulfate ratios (12.0, 10.0 and 7.5) on both cod removal and methane (ch4) production from sugarcane vinasse ad. batch assays were conducted in thermophilic conditions. at a cod/sulfate ...201728214696
biochemical composition of symplastic sap from sugarcane genetically modified to overproduce proline.global interest in sugarcane has increased significantly in recent years because of its economic impact on sustainable energy production. the purpose of the present study was to evaluate changes in the concentrations of total sugars, amino acids, free proline, and total proteins by colorimetric analyses and nuclear magnetic resonance (nmr) to perform a metabolic profiling of a water-soluble fraction of symplastic sap in response to the constitutive expression of a mutant δ(1)-pyrroline-5-carboxy ...201728213180
effect of biogas sparging on the performance of bio-hydrogen reactor over a long-term operation.this study aimed to enhance hydrogen production from sugarcane syrup by biogas sparging. two-stage continuous stirred tank reactor (cstr) and upflow anaerobic sludge blanket (uasb) reactor were used to produce hydrogen and methane, respectively. biogas produced from the uasb was used to sparge into the cstr. results indicated that sparging with biogas increased the hydrogen production rate (hpr) by 35% (from 17.1 to 23.1 l/l.d) resulted from a reduction in the hydrogen partial pressure. a fluctu ...201728207755
development of a low-cost cellulase production process using trichoderma reesei for brazilian biorefineries.during the past few years, the first industrial-scale cellulosic ethanol plants have been inaugurated. although the performance of the commercial cellulase enzymes used in this process has greatly improved over the past decade, cellulases still represent a very significant operational cost. depending on the region, transport of cellulases from a central production facility to a biorefinery may significantly add to enzyme cost. the aim of the present study was to develop a simple, cost-efficient ...201728184245
d-mannitol sensor based on molecularly imprinted polymer on electrode modified with reduced graphene oxide decorated with gold nanoparticles.an electrochemical sensor for d-mannitol based on molecularly imprinted polymer on electrode modified with reduced graphene oxide decorated with gold nanoparticles was developed in this present work. the sensor was constructed for the first time via the electropolymerization of o-phenylenediamine (o-pd) over a surface containing reduced graphene oxide (rgo) and gold nanoparticles (aunp) in the presence of d-mannitol molecules. the surface modification with aunp/rgo-gce facilitated the charge tra ...201728153247
comet assay and micronucleus tests on oreochromis niloticus (perciforme: cichlidae) exposed to raw sugarcane vinasse and to phisicochemical treated vinasse by ph adjustment with lime (cao).in brazil vinasse, a main sugarcane distillery residue, stands out because every liter of alcohol generates 10-15 l of vinasse as waste. an alternative for the disposal of this waste is the fertirrigation of the sugarcane culture itself. however, the high amount released can saturate the soil and through leaching/percolation contaminate water resources. the aim of this study is verifying the toxic potential of vinasse in tilapias and effectiveness of the physicalchemical treatment of this waste ...201728131919
analysis of three sugarcane homo/homeologous regions suggests independent polyploidization events of saccharum officinarum and saccharum spontaneum.whole genome duplication has played an important role in plant evolution and diversification. sugarcane is an important crop with a complex hybrid polyploid genome, for which the process of adaptation to polyploidy is still poorly understood. in order to improve our knowledge about sugarcane genome evolution and the homo/homeologous gene expression balance, we sequenced and analyzed 27 bacs (bacterial artificial chromosome) of sugarcane r570 cultivar, containing the putative single-copy genes lf ...201728082603
a kinetic model for hydrothermal pretreatment of sugarcane straw.this work presents kinetic models of cellulose and hemicellulose extraction during hydrothermal pretreatment of sugarcane straw. biomass was treated under conditions of 180, 195, and 210°c, using a solid/liquid ratio of 1:10 (w/v). in this study, cellobiose, glucose, formic acid and hydroxymethylfurfural (from cellulosic fraction) and xylose, arabinose, acetic acid, glucuronic acid and furfural (from hemicellulosic fraction) were taken into account in the kinetic parameters determination. the gl ...201728063360
microalgae cultivation in sugarcane vinasse: selection, growth and biochemical characterization.sugarcane ethanol is produced at large scale generating wastes that could be used for microalgae biomass production in a biorefinery strategy. in this study, forty microalgae strains were screened for growth in sugarcane vinasse at different concentrations. two microalgae strains, micractinium sp. embrapa|lba32 and c. biconvexa embrapa|lba40, presented vigorous growth in a light-dependent manner even in undiluted vinasse under non-axenic conditions. microalgae strains presented higher biomass pr ...201728061395
impacts of converting low-intensity pastureland to high-intensity bioenergy cropland on the water quality of tropical streams in brazil.in brazil, the cultivation of bioenergy crops is expanding at an accelerated rate. most of this expansion has occurred over low-intensity pasture and is considered sustainable because it does not involve deforestation of natural vegetation. however, the impacts on the water quality of headwater streams are poorly understood, especially with regard to the influence of land use patterns in the watershed. in this study, we investigated the effects of land-use conversion on the water quality of stre ...201728040217
a lignocellulosic hydrolysate-tolerant aurantiochytrium sp. mutant strain for docosahexaenoic acid production.to utilize lignocellulosic hydrolysate for docosahexaenoic acid (dha) production, a novel mutant aurantiochytrium sp. fn21 with strong tolerance against inhibitory lignocellulosic hydrolysate was obtained through continuous domestication processes from the parent strain aurantiochytrium sp. fju-512. aurantiochytrium sp. fn21 can accumulate 21.3% and 30.7% more dha compared to its parent strain cultured in fermentation medium and a medium with 50% (v/v) sugarcane bagasse hydrolysate (sbh), respec ...201728038399
in planta production and characterization of a hyperthermostable gh10 xylanase in transgenic sugarcane.sugarcane (saccharum sp. hybrids) is one of the most efficient and sustainable feedstocks for commercial production of fuel ethanol. recent efforts focus on the integration of first and second generation bioethanol conversion technologies for sugarcane to increase biofuel yields. this integrated process will utilize both the cell wall bound sugars of the abundant lignocellulosic sugarcane residues in addition to the sucrose from stem internodes. enzymatic hydrolysis of lignocellulosic biomass in ...201728005227
functional analysis of oxidative burst in sugarcane smut-resistant and -susceptible genotypes.smut pathogen induced an early modulation of the production and scavenging of reactive oxygen species during defence responses in resistant sugarcane that coincided with the developmental stages of fungal growth. sporisorium scitamineum is the causal agent of sugarcane smut disease. in this study, we characterized sugarcane reactive oxygen species (ros) metabolism in response to the pathogen in smut-resistant and -susceptible genotypes. sporisorium scitamineum teliospore germination and appresso ...201728004180
detection of bacillus and stenotrophomonas species growing in an organic acid and endocrine-disrupting chemical-rich environment of distillery spent wash and its phytotoxicity.sugarcane molasses-based distillery spent wash (dsw) is well known for its toxicity and complex mixture of various recalcitrant organic pollutants with acidic ph, but the chemical nature of these pollutants is unknown. this study revealed the presence of toxic organic acids (butanedioic acid bis(tms)ester; 2-hydroxysocaproic acid; benzenepropanoic acid, α-[(tms)oxy], tms ester; vanillylpropionic acid, bis(tms)), and other recalcitrant organic pollutants (2-furancarboxylic acid, 5-[[(tms)oxy] met ...201728000121
increased sink strength offsets the inhibitory effect of sucrose on sugarcane photosynthesis.spraying sucrose inhibits photosynthesis by impairing rubisco activity and stomatal conductance (gs), whereas increasing sink demand by partially darkening the plant stimulates sugarcane photosynthesis. we hypothesized that the stimulatory effect of darkness can offset the inhibitory effect of exogenous sucrose on photosynthesis. source-sink relationship was perturbed in two sugarcane cultivars by imposing partial darkness, spraying a sucrose solution (50mm) and their combination. five days afte ...201727889522
biological pretreatment of sugarcane bagasse with basidiomycetes producing varied patterns of biodegradation.this work evaluated sugarcane bagasse pretreatment with wood-decay fungi, producing varied patterns of biodegradation. the overall mass balance of sugars released after pretreatment and enzymatic hydrolysis indicated that a selective white-rot was necessary to provide glucose yields similar to the ones observed from leading physico-chemical pretreatment technologies. the selective white-rot ceriporiopsis subvermispora was selective for lignin degradation in the lignocellulosic material, preserve ...201727875764
transgenic sugarcane overexpressing canecpi-1 negatively affects the growth and development of the sugarcane weevil sphenophorus levis.transgenic sugarcane expressing canecpi-1 exhibits resistance to sphenophorus levis larvae. transgenic plants have widely been used to improve resistance against insect attack. sugarcane is an economically important crop; however, great losses are caused by insect attack. sphenophorus levis is a sugarcane weevil that digs tunnels in the stem base, leading to the destruction of the crop. this insect is controlled inefficiently by chemical insecticides. transgenic plants expressing peptidase inhib ...201727837261
relative toxicity of two aphicides to hippodamia convergens (coleoptera: coccinellidae): implications for integrated management of sugarcane aphid, melanaphis sacchari (hemiptera: aphididae).flupyradifurone and sulfoxaflor present novel insecticide chemistries with particular efficacy against aphids, and the recent emergence of sugarcane aphid, melanaphis sacchari (zehntner), as a pest of sorghum in the united states has resulted in their widespread use. we examined their toxicity to hippodamia convergens guerin-meneville, an important aphid biocontrol agent. we exposed beetles to topical applications of the field rate (fr) of these insecticides, fed them contaminated food (eggs of ...201728039423
fabrication, optimization, and characterization of noble silver nanoparticles from sugarcane leaf (saccharum officinarum) extract for antifungal application.metal nanoparticles obtained from green route are gaining significant prominence as a result of their potential applications in nanomedicine and material engineering. overall metal nanoparticles studied, silver nanoparticles (agnps) clutch prominent place in nanoparticles research field. herein, we have reported the green synthesis of saccharum officinarum leaf biomass extract-mediated synthesis of agnps. initial nanoparticle production was confirmed by visual observation as color change from li ...201728597158
influence of agricultural activities, forest fires and agro-industries on air quality in thailand.annual and monthly-based emission inventories in northern, central and north-eastern provinces in thailand, where agriculture and related agro-industries are very intensive, were estimated to evaluate the contribution of agricultural activity, including crop residue burning, forest fires and related agro-industries on air quality monitored in corresponding provinces. the monthly-based emission inventories of air pollutants, or, particulate matter (pm), nox and so2, for various agricultural crops ...201728254062
development of the crop residue and rangeland burning in the 2014 national emissions inventory using information from multiple sources.biomass burning has been identified as an important contributor to the degradation of air quality because of its impact on ozone and particulate matter. one component of the biomass burning inventory, crop residue burning, has been poorly characterized in the national emissions inventory (nei). in the 2011 nei, wildland fires, prescribed fires, and crop residue burning collectively were the largest source of pm2.5. this paper summarizes our 2014 nei method to estimate crop residue burning emissi ...201727964698
molecular characterization and co-expression analysis of the snrk2 gene family in sugarcane (saccharum officinarum l.).in plants, both abscisic acid (aba) dependent and independent pathways form the basis for the response to environmental stresses. sucrose non-fermenting 1-related protein kinase 2 (snrk2) plays a central role in plant stress signal transduction. however, complete annotation and specific expression patterns of snrk2s in sugarcane remain unclear. for the present study, we performed a full-length cdna library survey of sugarcane, thus identifying ten sosnrk2 genes via phylogenetic, local blast meth ...201729247208
identification of udp-glucose binding site in glycosyltransferase domain of sucrose phosphate synthase from sugarcane (saccharum officinarum) by structure-based site-directed mutagenesis.sucrose phosphate synthase (sps) is believed to be the key enzyme for controlling the biosynthesis of sucrose. spss consist of a functional glycosyltransferase domain that shares conserved residues with the glycosyltransferase domain of sucrose biosynthesis-related protein. the formation of sucrose-6-phosphate is catalyzed by sps with the transfer of a glycosyl group of uridine diphosphate glucose (udp-g) as an activated donor sugar to a fructose-6-phosphate as a sugar acceptor. however, underst ...201729222806
association of variation in the sugarcane transcriptome with sugar content.sugarcane is a major crop of the tropics cultivated mainly for its high sucrose content. the crop is genetically less explored due to its complex polyploid genome. sucrose synthesis and accumulation are complex processes influenced by physiological, biochemical and genetic factors, and the growth environment. the recent focus on the crop for fibre and biofuel has led to a renewed interest on understanding the molecular basis of sucrose and biomass traits. this transcriptome study aimed to identi ...201729178834
cell wall metabolism and hexose allocation contribute to biomass accumulation in high yielding extreme segregants of a saccharum interspecific f2 population.sugarcane is an emerging dual-purpose biofuel crop for energy and sugar production, owing to its rapid growth rate, high sucrose storage in the stems, and high lignocellulosic yield. it has the highest biomass production reaching 1.9 billion tonnes in 2014 worldwide.201729020919
genome-wide identification of leaf abscission associated micrornas in sugarcane (saccharum officinarum l.).sugarcane (saccharum officinarum l.) is an economically important crop, mainly due to the production of sugar and biofuel (azevedo ra, carvalho rf, cia mc, & gratão pl, trop plant biol 4:42-51, 2011). grown mainly in tropical and subtropical countries, sugarcane is a highly polyploid plant with up to ten copies of each chromosome, which increases the difficulties of genome assembly and genetic, physiologic and biochemical analyses. the increasing demands of sugar and the increasing cost of sugar ...201728946845
luxurious nitrogen fertilization of two sugar cane genotypes contrasting for lignin composition causes changes in the stem proteome related to carbon, nitrogen, and oxidant metabolism but does not alter lignin content.sugar cane is an important crop for sugar and biofuel production. its lignocellulosic biomass represents a promising option as feedstock for second-generation ethanol production. nitrogen fertilization can affect differently tissues and its biopolymers, including the cell-wall polysaccharides and lignin. lignin content and composition are the most important factors associated with biomass recalcitrance to convert cell-wall polysaccharides into fermentable sugars. thus it is important to understa ...201728836437
a dynamic hydro-mechanical and biochemical model of stomatal conductance for c4 photosynthesis.c4 plants are major grain (maize [zea mays] and sorghum [sorghum bicolor]), sugar (sugarcane [saccharum officinarum]), and biofuel (miscanthus spp.) producers and contribute ∼20% to global productivity. plants lose water through stomatal pores in order to acquire co2 (assimilation [a]) and control their carbon-for-water balance by regulating stomatal conductance (gs). the ability to mechanistically predict gs and a in response to atmospheric co2, water availability, and time is critical for simu ...201728751312
scale insects (hemiptera: coccomorpha) on sugarcane in colombia, with description of a new species of tillancoccus ben-dov (coccidae).herein we describe a new species, tillancoccus koreguajae caballero & ramos, sp. n. (hemiptera: coccidae) from colombia collected on sugarcane. pinnaspis strachani (cooley) is also recorded on sugarcane for the first time worldwide. an updated list of seven species of coccomorpha on sugarcane in colombia is provided, including information on its distribution, biology, and mutualistic ants for each species. seven species of scale insects (hemiptera: coccomorpha) have been recorded previously on s ...201728609908
a survey of the complex transcriptome from the highly polyploid sugarcane genome using full-length isoform sequencing and de novo assembly from short read sequencing.despite the economic importance of sugarcane in sugar and bioenergy production, there is not yet a reference genome available. most of the sugarcane transcriptomic studies have been based on saccharum officinarum gene indices (sogi), expressed sequence tags (ests) and de novo assembled transcript contigs from short-reads; hence knowledge of the sugarcane transcriptome is limited in relation to transcript length and number of transcript isoforms.201728532419
plant regeneration from cell suspension culture in saccharum officinarum l. and ascertaining of genetic fidelity through rapd and issr markers.the aim of this study was to produce sugarcane plantlets from cell suspension culture and study its genetic fidelity using molecular markers. the study was carried out using sugarcane varieties co 86032 and q117. callus cultures of both the varieties were optimized using six different callus induction media. after screening the growth response of callus on six different callus induction media, it was observed that medium no. vi supplemented with 500 mg l-1 of each pvp, casein hydrolysate and mes ...201728391480
physicochemical and sensory (aroma and colour) characterisation of a non-centrifugal cane sugar ("panela") beverage.non-centrifugal cane sugar (ncs), also called "panela", is a high carbohydrate-content food obtained by boil evaporation of the sugar cane juice. this study was undertaken to assess physicochemical properties and sensory characteristics of panela beverage at two different concentrations. evaluation of ph, °brix, and colour (tristimulus colorimetry) was carried out in all panela drink samples. in order to characterise the odour-active volatiles of the beverage, a simultaneous steam distillation-s ...201728317778
effect of spray drying on physical properties of sugarcane juice powder (saccharum officinarum l.).the aim of the present study was to investigate the spray drying behavior of sugarcane juice with (psj) and without (csj) citric acid the effects of different levels (10-50%) of carrier agents (maltodextrin (md), gum arabic, liquid glucose and carrot fiber) at varying operating conditions of inlet and outlet temperature and feed concentration during spray drying was also studied. spray dried powders from psj and csj were analyzed for physical properties such as wettability, cohesiveness, dispers ...201728298682
effect of sugar industry spentwash (diluted) on the characteristics of soil and sugarcane (saccharum officinarum l.) growth in the subtropical environment of sindh, pakistan.spentwash is a rich source of organic matter and essential plant nutrients in addition to excess salts. sugar mills in pakistan discharge about 3.48 million tons of spentwash annually, with no proper procedures for its disposal or utilization. to test the effect of diluted spentwash on soil and the soil's ability to support plant growth, sugarcane (saccharum officinarum l.) variety cpf-237 was planted. the experiment was conducted in a randomized complete block design involving factorial combina ...201728243931
early selection of sugarcane using path analysis.the aim of this study was to analyze sugarcane (saccharum officinarum) biometric and technological data, obtained at different timepoints, using path analysis. the experiment was conducted in união, pi, brazil, and evaluated 12 sugarcane genotypes (rb036066, rb9438, rb935744, rb021764, rb021754, rb021534, rb966229, rb977540, rb863129, and rb987935, and the varieties rb92579 and rb867515 as controls) in a randomized block design with four replications. data were collected at six timepoints that w ...201728198498
complete chloroplast genomes of erianthus arundinaceus and miscanthus sinensis: comparative genomics and evolution of the saccharum complex.the genera erianthus and miscanthus, both members of the saccharum complex, are of interest as potential resources for sugarcane improvement and as bioenergy crops. recent studies have mainly focused on the conservation and use of wild accessions of these genera as breeding materials. however, the sequence data are limited, which hampers the studies of phylogenetic relationships, population structure, and evolution of these grasses. here, we determined the complete chloroplast genome sequences o ...201728125648
comparison between ground ant (hymenoptera: formicidae) communities foraging in the straw mulch of sugarcane crops and in the leaf litter of neighboring forests.in many sugarcane plantations in brazil, the straw is left on the soil after harvesting, and vinasse, a by-product of the production of sugar and ethanol, is used for fertigation. our goal was to compare ant community composition and species richness in the straw mulch of sugarcane crops with the leaf litter of neighboring forests. we tested the hypothesis that ant communities in the straw mulch of vinasse-irrigated sugarcane crops and in the forest leaf litter were similar, because the combinat ...201728053209
new subgroup 16srxi-f phytoplasma strain associated with sugarcane grassy shoot (scgs) disease in india.a novel phytoplasma subgroup strain sc-phy385 (genbank accession number hf586648) was found associated with sugarcane grassy shoot (scgs) disease in india. sugarcane (saccharum officinarum l.) leaf samples with 'grassy shoot' symptoms were collected from the western region of maharashtra state. the analysis based on restriction fragment length polymorphism (rflp) profiles and 16s rrna gene sequences indicated that most of the phytoplasma strains belong to the 16srxi-b group. however, scgs phytop ...201727902251
multi-trait multi-environment quantitative trait loci mapping for a sugarcane commercial cross provides insights on the inheritance of important traits.breeding trials typically consist of phenotypic observations for various traits evaluated in multiple environments. for sugarcane in particular, repeated measures are obtained for plant crop and one or more ratoons, such that joint analysis through mixed models for modeling heterogeneous genetic (co)variances between traits, locations and harvests is appropriate. this modeling approach also enables us to include molecular marker information, aiding in understanding the genetic architecture of qu ...201726273212
gh10 xyna is the main xylanase identified in the crude enzymatic extract of paenibacillus sp. a59 when grown on xylan or lignocellulosic biomass.a novel bacterial isolate with polysaccharides degrading activity was identified as paenibacillus sp., and named paenibacillus sp. a59. even though it is a strict mesophile, optimal xylanase activity of the crude enzymatic extract was achieved between 50°c and 70°c and more than 60% of the activity was retained after incubation for 48h at 50°c, indicating thermotolerance of the enzymes involved. the extract was also active on pre-treated sugarcane residue (scr) and wheat straw, releasing xylobio ...201727242139
histopathological analysis of tilapia gills (oreochromis niloticus linnaeus, 1758) exposed to sugarcane vinasse.sugarcane vinasse is one of the main residues generated by the transformation of cane into ethanol. because of the high organic content (cod), high biochemical oxygen demand (bod), low ph, the large amount that this residue is generated (15l for every liter of ethanol produced) and their use as fertilizer on the sugarcane crop, this residue is potentially polluting to the soil ecossystem and by percolation to water ecossystem too. thus, this study aimed to assess the toxicity of vinasse by analy ...201727770647
comprehensive analysis of the cellulolytic system reveals its potential for deconstruction of lignocellulosic biomass in a novel streptomyces sp.the giant snail achatina fulica is considered an invasive species in most territories in which it was introduced, due to its ability to process a large amount of lignocellulose as a consequence of the presence of a cellulolytic-associated microflora. streptomyces are well known as crucial agents in the decomposition of complex polymers in soil environments and also as cellulolytic symbionts commonly associated with herbivore insects. here, we employed a combination of genomic and biochemical too ...201727699477
effect of sugarcane biopolymer gel injected in rabbit vocal fold.alterations in the vocal folds that involve volume reduction and glottal closure failure result in exaggerated air escape during speech. for such situations, the use of implants or grafts of different materials has been proposed.201725153106
thermotolerant and mesophylic fungi from sugarcane bagasse and their prospection for biomass-degrading enzyme production.nineteen fungi and seven yeast strains were isolated from sugarcane bagasse piles from an alcohol plant located at brazilian cerrado and identified up to species level on the basis of the gene sequencing of 5.8s-its and 26s ribosomal dna regions. four species were identified: kluyveromyces marxianus, aspergillus niger, aspergillus sydowii and aspergillus fumigatus, and the isolates were screened for the production of key enzymes in the saccharification of lignocellulosic material. among them, th ...201726413077
chemical input reduction in the arabinoxylan and lignocellulose alkaline extraction and xylooligosaccharides production.lignocellulosic material breakdown by hydrolysis is an important step to open new perspectives for bioenergy and special foods production like prebiotic xylooligosaccharides. improvement of lignocellulose and arabinoxylan alkaline extraction from sugarcane bagasse and enzymatic hydrolysis were performed. treatments 1 (10% koh at 70°c), 3 (5% koh at 121°c) and zd method (24% koh at 35°c) showed solid lignocellulose recovery of respectively 75.2%, 74.2% and 73%. a range of 24.8-27% extracted mater ...201728063358
global analysis of mannitol 2-dehydrogenase in lactobacillus reuteri crl 1101 during mannitol production through enzymatic, genetic and proteomic approaches.several plants, fungi, algae, and certain bacteria produce mannitol, a polyol derived from fructose. mannitol has multiple industrial applications in the food, pharmaceutical, and medical industries, being mainly used as a non-metabolizable sweetener in foods. many heterofermentative lactic acid bacteria synthesize mannitol when an alternative electron acceptor such as fructose is present in the medium. in previous work, we reported the ability of lactobacillus reuteri crl 1101 to efficiently pr ...201728060932
d-xylose fermentation, xylitol production and xylanase activities by seven new species of sugiyamaella.sixteen yeast isolates identified as belonging to the genus sugiyamaella were studied in relation to d-xylose fermentation, xylitol production, and xylanase activities. the yeasts were recovered from rotting wood and sugarcane bagasse samples in different brazilian regions. sequence analyses of the internal transcribed spacer (its) region and the d1/d2 domains of large subunit rrna gene showed that these isolates belong to seven new species. the species are described here as sugiyamaella ayubii ...201727688209
biosynthesis, purification and characterization of β-1,4-xylanase from a novel mangrove associated actinobacterium streptomyces olivaceus (msu3) and its applications.the present study was undertaken to evaluate the xylanolytic properties of an actinobacterium streptomyces olivaceus (msu3) isolated from the sediment sample of mangrove environment. it showed highest xylanase activity on initial screening in breg's mineral salts medium supplemented with 0.5% xylan. further the organism expressed maximum xylanase production at optimized culture conditions of ph 7, temperature 30 °c with 2.5% of inoculum size at 72 h of incubation, the nutrient sources like sucro ...201727693622
meta-analysis of effects of inoculation with homofermentative and facultative heterofermentative lactic acid bacteria on silage fermentation, aerobic stability, and the performance of dairy cows.forages are usually inoculated with homofermentative and facultative heterofermentative lactic acid bacteria (lab) to enhance lactic acid fermentation of forages, but effects of such inoculants on silage quality and the performance of dairy cows are unclear. therefore, we conducted a meta-analysis to examine the effects of lab inoculation on silage quality and preservation and the performance of dairy cows. a second objective was to examine the factors affecting the response to silage inoculatio ...201728342607
further characterization of maize chlorotic mottle virus and its synergistic interaction with sugarcane mosaic virus in maize.maize chlorotic mottle virus (mcmv) was first reported in maize in china in 2009. in this study we further analyzed the epidemiology of mcmv and corn lethal necrosis disease (clnd) in china. we determined that clnd observed in china was caused by co-infection of mcmv and sugarcane mosaic virus (scmv). phylogenetic analysis using four full-length mcmv cdna sequences obtained in this study and the available mcmv sequences retrieved from genbank indicated that chinese mcmv isolates were derived fro ...201728059116
iranian johnsongrass mosaic virus: the complete genome sequence, molecular and biological characterization, and comparison of coat protein gene sequences.iranian johnsongrass mosaic virus (ijmv) is one of the most prevalent viruses causing maize mosaic disease in iran. an ijmv isolate, maz-bah, was obtained from the maize showing mosaic symptoms in mazandaran, north of iran. the complete genomic sequence of maz-bah is 9544 nucleotides, excluding the poly(a) tail. it contains one single open reading frame of 9165 nucleotides and encodes a large polyprotein of 3054 amino acids, flanked by a 5'-untranslated region (utr) of 143 nucleotides and a 3'-u ...201727632283
species diversity and population dynamics of entomopathogenic fungal species in the genus metarhizium-a spatiotemporal study.we studied the species diversity and population genetic structure of isolates of fungi from the entomopathogenic genus metarhizium that had been isolated from sugarcane crops and surrounding grass. soil and leaf samples were taken on four sampling occasions over 13 months (october 2014-october 2015). isolations were made using the galleria mellonella baiting method and selective media. phylogenetic placement of isolates was done by sequencing a fragment of the 5' of the elongation factor 1-α gen ...201728124726
how native yeasts may influence the chemical profile of the brazilian spirit, cachaça?cachaça is the typical brazilian spirit, obtained by distillation of fermented sugarcane must, whose production is closely related to regional heritage and geographical traits. in this work, we investigate the influence of different autochthonous yeast species, in comparison to other commercial strains, on the chemical profile of cachaça. fermentations were performed with four different wild species, a mixed starter culture with native yeasts, and two commercial strains. procedures were carried ...201728290322
use of pervaporation process for the recovery of aroma compounds produced by p. fermentans in sugarcane molasses.natural fruity aroma was produced during submerged fermentation by pichia fermentans using sugarcane molasses as a cultivation broth. the aroma compounds were recovered from the fermentation by a pervaporation process using a polydimethylsiloxane membrane (pervap 4060-sulzer). isoamyl acetate, a characteristic compound associated with fruity aromas, was the major compound produced. the pervaporation module was fed at three different temperatures to test the best conditions to recover the natural ...201728303325
deletion of ph regulator pac-3 affects cellulase and xylanase activity during sugarcane bagasse degradation by neurospora crassa.microorganisms play a vital role in bioethanol production whose usage as fuel energy is increasing worldwide. the filamentous fungus neurospora crassa synthesize and secrete the major enzymes involved in plant cell wall deconstruction. the production of cellulases and hemicellulases is known to be affected by the environmental ph; however, the regulatory mechanisms of this process are still poorly understood. in this study, we investigated the role of the ph regulator pac-3 in n. crassa during t ...201728107376
lipids of rhodotorula mucilaginosa iipl32 with biodiesel potential: oil yield, fatty acid profile, fuel properties.this study analyzes the single cell oil (sco), fatty acid profile, and biodiesel fuel properties of the yeast rhodotorula mucilaginosa iipl32 grown on the pentose fraction of acid pre-treated sugarcane bagasse as a carbon source. the yeast biomass from nitrogen limiting culture conditions (15.3 g l(-1) ) was able to give the sco yield of 0.17 g g(-1) of xylose consumed. acid digestion, cryo-pulverization, direct in situ transesterification, and microwave assisted techniques were evaluated in com ...201728155998
draft genome sequence of saccharomyces cerevisiae barra grande (bg-1), a brazilian industrial bioethanol-producing strain.here, we present the draft genome sequence of saccharomyces cerevisiae bg-1, a brazilian industrial strain widely used for bioethanol production from sugarcane. the 11.7-mb genome sequence consists of 216 scaffolds and harbors 5,607 predicted protein-coding genes.201728360170
microbial-enhanced lindane removal by sugarcane (saccharum officinarum) in doped soil-applications in phytoremediation and bioaugmentation.the aim of this study was to examine the effect of lindane-degrading yeast on the growth and lindane uptake by saccharum sp., in doped garden soils. the rhizosphere of saccharum plant was amended with yeast candida vitjzn04 by root-inoculation. the bio-augment yeast was applied in two different forms viz., planktonic form and cells immobilized on sugarcane-bagasse, in the pot experiments. garden soils (lindane∼100 mg/kg) exposed to various treatments were monitored for a period of 30 days, for r ...201728259469
the coptotermes gestroi aldo-keto reductase: a multipurpose enzyme for biorefinery applications.in nature, termites can be considered as a model biological system for biofuel research based on their remarkable efficiency for lignocellulosic biomass conversion. redox enzymes are of interest in second-generation ethanol production because they promote synergic enzymatic activity with classical hydrolases for lignocellulose saccharification and inactivate fermentation inhibitory compounds produced after lignocellulose pretreatment steps.201728053664
use of lignocellulose biomass for endoxylanase production by streptomyces termitum.actinobacteria isolates from brazilian cerrado soil were evaluated for their ability to produce enzymes of the cellulolytic and xylanolytic complex using lignocellulose residual biomass. preliminary semiquantitative tests, made in petri plates containing carboxymethylcellulose and beechwood xylan, indicated 11 potential species producing enzymes, all belonging to the genus streptomyces. the species were subsequently grown in pure substrates in submerged fermentation and analyzed for the producti ...201728045607
alleviation of heavy metals toxicity by the application of plant growth promoting rhizobacteria and effects on wheat grown in saline sodic field.the aim of the study was to determine tolerance of plant growth promoting rhizobacteria (pgpr) in different concentrations of cu, cr, co, cd, ni, mn, and pb and to evaluate the pgpr-modulated bioavailability of different heavy metals in the rhizosphere soil and wheat tissues, grown in saline sodic soil. bacillus cereus and pseudomonas moraviensis were isolated from cenchrus ciliaris l. growing in the khewra salt range. seven-day-old cultures of pgpr were applied on wheat as single inoculum, co-i ...201727936865
low-melanin containing pullulan production from sugarcane bagasse hydrolysate by aureobasidium pullulans in fermentations assisted by light-emitting diode.pullulan is a polymer produced by aureobasidium pullulans and the main bottleneck for its industrial production is the presence of melanin pigment. in this study, light-emitting diodes (leds) of different wavelengths were used to assist the fermentation process aiming to produce low-melanin containing pullulan by wild strain of a. pullulans lb83 with different carbon sources. under white light using glucose-based medium, 11.75g.l(-1) of pullulan with high melanin content (45.70ua540nm.g(-1)) was ...201728161623
production of poly(malic acid) from sugarcane juice in fermentation by aureobasidium pullulans: kinetics and process economics.poly(β-l-malic acid) (pma) is a biodegradable polymer with many potential biomedical applications. pma can be readily hydrolyzed to malic acid (ma), which is widely used as an acidulant in foods and pharmaceuticals. pma production from sucrose and sugarcane juice by aureobasidium pullulans zx-10 was studied in shake-flasks and bioreactors, confirming that sugarcane juice can be used as an economical substrate without any pretreatment or nutrients supplementation. a high pma titer of 116.3g/l and ...201727839861
prospecting agro-waste cocktail: supplementation for cellulase production by a newly isolated thermophilic b. licheniformis 2d55.bacteria isolated from thermophilic environment that can produce cellulase as well as utilise agro-waste biomass have a high potential for developing thermostable cellulase required in the biofuel industry. the cost for cellulase represents a significant challenge in converting lignocellulose to fermentable sugars for biofuel production. among three potential bacteria examined, bacillus licheniformis 2d55 (accession no. kt799651) was found to produce the highest cellulolytic activity (cmcase 0.3 ...201728176140
expression of thermostable β-xylosidase in escherichia coli for use in saccharification of plant biomass.the present work is aimed to evaluate the saccharification potential of a thermostable β-xylosidase cloned from bacillus licheniformis into escherichia coli for production of bioethanol from plant biomass. recombinant β-xylosidase enzyme possesses the ability of bioconversion of plant biomass like wheat straw, rice straw and sugarcane bagass. by using this approach, plant biomass that mainly constitute cellulose can be converted to reducing sugars that could then be easily converted to bioethano ...201728140759
optimization of levan production by cold-active bacillus licheniformis ant 179 and fructooligosaccharide synthesis by its levansucrase.fructooligosaccharides (fos) and levan attract much attention due to a wide range of applications in food technology and pharmaceutical and cosmetic industry. bacillus licheniformis ant 179, isolated from antarctica soil, produced levansucrase and levan in a medium containing sucrose as carbon substrate. in this study, characterization of levansucrase and production of short-chain fos and levan were investigated. temperature and ph optimum of the enzyme were found to be 60 °c and ph 6.0, respect ...201727734288
development of minimal enzyme cocktails for hydrolysis of sulfite-pulped lignocellulosic biomass.despite recent progress, saccharification of lignocellulosic biomass is still a major cost driver in biorefining. in this study, we present the development of minimal enzyme cocktails for hydrolysis of norway spruce and sugarcane bagasse, which were pretreated using the so-called bali™ process, which is based on sulfite pulping technology. minimal enzyme cocktails were composed using several glycoside hydrolases purified from the industrially relevant filamentous fungus trichoderma reesei and a ...201728219736
cry1ac and vip3aa proteins from bacillus thuringiensis targeting cry toxin resistance in diatraea flavipennella and elasmopalpus lignosellus from sugarcane.the biological potential of vip and cry proteins from bacillus is well known and widely established. thus, it is important to look for new genes showing different modes of action, selecting those with differentiated entomotoxic activity against diatraea flavipennella and elasmopalpus lignosellus, which are secondary pests of sugarcane. therefore, cry1 and vip3 proteins were expressed in escherichia coli, and their toxicities were evaluated based on bioassays using neonate larvae. of those, the m ...201728123906
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