Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| electron microscopic findings of the peripheral nerve lesions of nude mice inoculated with m. leprae. | peripheral nerve lesions of nude mice inoculated with mycobacterium leprae were examined from 1 year to 1 year and 8 months post-inoculation. leprosy bacilli and intracytoplasmic foamy structures were found in the perineurial cells, endothelial cells of capillaries, schwann cells, and axons of the myelinated nerve fibers inside the lepromas of foot pads. in the proximal portion of the sciatic nerve, bacilli were found chiefly in the macrophages of the endoneurial space. bacilli were not found in ... | 1985 | 3889194 |
| cultivable mycobacteria isolated from organs of armadillos uninoculated and inoculated with mycobacterium leprae. | mycobacteria were cultivated from 16 out of 32 samples of tissues from armadillos inoculated with mycobacterium leprae. three out of 7 samples from non-inoculated armadillos held in captivity were also positive for cultivable mycobacteria. some isolated strains belonged to the m. avium-intracellulare-scrofulaceum complex, while others were identified as m. gordonae and m. terrae. unclassified mycobacteria were isolated from m. leprae-inoculated armadillos only. taxonomic studies confirmed that t ... | 1985 | 3890695 |
| essential fatty acids in the plasma phospholipids of patients with leprosy. | plasma phospholipid essential fatty acids were investigated in 40 patients with leprosy and 40 controls. a significant reduction in linoleic acid was found in the leprosy patients, with an increase in its metabolite dihomo-gamma-linolenic acid. no difference was found between patients with multibacillary and paucibacillary leprosy. patients treated for less than 6 months were found to have low levels of linoleic acid and high levels of dihomo-gamma-linolenic and arachidonic acid compared with pa ... | 1985 | 3890922 |
| [osteitis leprosa multiplex cystica--an early symptom of lepromatous leprosy]. | bone lesions of the finger in a 20-year-old leprosy patient are described to show their diagnostic value. the aetiological differences between specific (caused by mycobacterium leprae itself) and unspecific osseal changes are morphologically noticeable in morbus hansen. the x-ray picture shows cystic lightening by granulomatous destruction and distal absorption of the bone. these lesions are typical, and radiological investigations might be the first step in the diagnosis of leprosy. in our case ... | 1985 | 3894289 |
| changes in circulating antibody levels to the major phenolic glycolipid during erythema nodosum leprosum in leprosy patients. | circulating antibody levels to the phenolic glycolipid from mycobacterium leprae and soluble m. leprae antigens were monitored before, during and following enl episodes in 12 patients. it was observed that during enl reaction, there was a fall in circulating antibody levels to the phenolic glycolipid but not to the soluble antigens from m. leprae. when the patients had recovered from their enl reactions, the anti-glycolipid antibody levels usually increased again to levels similar to those obser ... | 1985 | 3894538 |
| phagocytic and bactericidal activities of macrophages from mycobacterium leprae-infected normal and immunosuppressed mice. | phagocytic and bactericidal activities were studied in mycobacterium leprae-infected normal (ni) and thymectomized/irradiated (tri) mice at different time periods. no significant differences were seen in the phagocytic activity for staphylococcus aureus at 3, 6, and 9 months in the normal infected (ni) and normal control (nc) mice. a slight but significant decrease in the phagocytosis of s. aureus was seen in the tri as compared to the ni group at 3 months which recovered at 6 months. phagocytos ... | 1985 | 3894539 |
| an enzyme immunoassay titrating igm antibody against phenolic glycolipid for diagnosis of lepromatous leprosy. | 1985 | 3902635 | |
| electron microscopic findings of transverse fission of m. leprae by freeze-etching methods. | the structures of multiplication by transverse fission found in the lepromas and livers of nude mice and nine-banded armadillos inoculated with mycobacterium leprae isolated from human lepromas, a nine-banded armadillo with naturally acquired leprosy-like disease, and a nine-banded armadillo inoculated with m. leprae isolated from the mangabey monkey with naturally acquired leprosy infection are described. the images of multiplication by transverse fission of m. leprae were almost always found i ... | 1985 | 3894540 |
| an unusual case of untreated polar lepromatous leprosy associated with rare m. leprae. | 1985 | 3894542 | |
| the effect of mycobacterium leprae on pha- and ppd-induced inhibition of leukocyte migration in leprosy patients. | 1985 | 3894846 | |
| rapid, radiometric in vitro assay for the evaluation of the anti-leprosy activity of clofazimine and its analogues. | 1985 | 3894849 | |
| abstracts: first u.s. hansen's disease research conference. national hansen's disease center, carville, louisiana, u.s.a, 13-15 august 1954. | 1985 | 3889188 | |
| isoenzymes of mycobacteria. ii. relevance of ldh zymograms in taxonomy and identification. | the cell free extracts of mycobacteria namely m. kansasii m. avium, m. tuberculosis, bcg (glaxo), m. gastri, m. phlei, m. smegmatis, m. vaccae, m. strain w., m. scrofulaceum, m. gordonae, m. nonchromogenicum e. coli, staph, aureus, and m. leprae infected skin have been electrophoresed and stained for ldh activity. normal skin tissue was also taken as control. it was found that all the organisms tested showed distinct species specific ldh isoenzyme patterns. there was no extra band but an aberran ... | 1985 | 3897403 |
| serological survey of leprosy and control subjects by a monoclonal antibody-based immunoassay. | a serological antibody competition test (sact) using a murine anti-mycobacterium leprae-specific monoclonal antibody (ml04) has been evaluated in 96 untreated leprosy patients and 128 control subjects. the test is based on the competitive inhibition by antibodies from human test sera of the binding of the specific 125i-ml04 murine monoclonal antibody to m. leprae soluble antigen coated microtiter plates. along the spectrum of the disease, sact positivity was observed in 100% of ll-bl, 87.5% of b ... | 1985 | 3889189 |
| absence of mycobactin in mycobacterium leprae; probably a microbe dependent microorganism implications. | ferric mycobactins were prepared from mycobacterium phlei. mycobacterium avium--intracellulare a and h, isolated respectively from armadillo and human leprosy specimens. attempts were made to extract mycobactin from host grown m. leprae cells. the crude ferric mycobactin extracts were tested for growth supporting effect on the mycobactin dependent m. paratuberculosis strain atcc 19698. mycobactins prepared from m. phlei and the two m. avium--intracellulare strains had growth promoting effect on ... | 1985 | 3897405 |
| thymopentin treatment in patients with chemotherapy-resistant lepromatous leprosy. | leprosy is a chronic infectious disease caused by mycobacterium leprae; it is chiefly involving the skin and peripheral nerves. in lepromatous leprosy there are widespread loose infiltrates with m. leprae multiplying extensively in the skin macrophages and schwann cells of peripheral nerves. such patients reveal a decrease of circulating t helper cells, which is still more pronounced in the cutaneous lesions. due to the ever increasing bacterial resistance to classical dapsone and combined chemo ... | 1985 | 3898294 |
| comparative study on the thin-layer chromatographic pattern of methyl mycolates of m. leprae and related species. | the thin-layer chromatographic (tlc) pattern of methyl mycolates of mycobacterium leprae has been compared with the lipids extracted from m. leprae-infected tissue material and also with those of other cultivable mycobacterial species. it has been found that m. leprae-derived methyl mycolates give two spots, namely, alpha- and keto-derivatives, after charring the tlc plates with k2cr2o7/h2so4 spray. lipids extracted from infected tissue material also show spots with the same rf values when compa ... | 1985 | 3889190 |
| detection of phenolic glycolipid i in sera from patients with lepromatous leprosy. | 1985 | 3900237 | |
| an electron microscopic study on macrophages and lymphocytes in lepromatous and borderline leprosy. | we describe how macrophages are activated and phagocytose mycobacteria in lepromatous leprosy. the differentiation of macrophages into epithelioid cells and into giant cells in borderline leprosy is shown. close apposition between macrophages and lymphocytes is seen in those areas where mycobacteria disintegrate inside macrophages. | 1985 | 3889192 |
| results from cation and mass fingerprint analysis of single cells and from atp measurements of m. leprae for drug sensitivity testing: a comparison. | the physiologic states of mycobacterium leprae isolated from patient biopsies were studied using single cell mass spectrometry by laser microprobe mass analysis (lam-ma) and atp bioluminescence assay. the changes in the physiologic state of m. leprae after the patients had been treated with dapsone (dds) monotherapy were also studied. the shift of the low intracellular na+, k+-ratio of untreated m. leprae cells to higher values under dds therapy, as measured from a limited number of single bacte ... | 1985 | 3900243 |
| an electron microscopic study of the small cutaneous vessels in lepromatous leprosy. | the small dermal vessels play an important role for the propagation of mycobacterium leprae. bacteria can multiply in the endothelial cells as well as in the pericytes and can enter the blood stream from the endothelial cells and the interstitial space from the pericytes. m. leprae can be phagocytosed by blood monocytes. | 1985 | 3889193 |
| leprosy in a mangabey monkey--naturally acquired infection. | naturally acquired leprosy was detected in an otherwise normal "sooty" mangabey monkey (cercocebus atys). this animal was imported from west africa in 1975 and developed clinical symptoms of leprosy in 1979. histopathologic findings were those of subpolar-lepromatous to borderline-lepromatous leprosy in the ridley-jopling classification. the disease was progressive, with crippling neuropathic deformities of the hands and feet. the disease regressed under specific therapy. the etiologic agent was ... | 1985 | 3889184 |
| dds-resistant leprosy. | 1985 | 3889186 | |
| drug sensitivity testing of m. leprae. | 1985 | 3889187 | |
| microscopic findings of delayed reactions elicited by the skin test reagent leprosin a derived from m. leprae. | punch biopsies taken 12, 24, 48, and 72 hours after skin testing with leprosin a have been used to prepare ultrathin sections for the identification and enumeration of infiltration cells. the study was performed on small numbers of both healthy persons and leprosy patients with various forms of the disease living in india. similar cells were found to infiltrate both positive and negative responses to the skin test reagent, although there were quantitative differences. the most striking findings ... | 1985 | 3900246 |
| in vivo responses to mycobacterium leprae: antigen presentation, interleukin-2 production, and immune cell phenotypes in naturally occurring leprosy lesions. | to investigate the immune defect in lepromatous leprosy we studied immune cell phenotypes, lymphocyte activation states, and interleukin-2 (il-2) production in naturally occurring leprosy skin lesions. mouse hybridoma monoclonal antibodies reacting with the il-2 receptor (anti-tac), unbound il-2 (dms-1), antigen-presenting langerhans' cells (okt6) and the okt4-leu3 and okt8 t-lymphocyte subpopulations were used with indirect horseradish peroxidase and alkaline phosphatase techniques on frozen bi ... | 1985 | 3900245 |
| suppression of mycobacterium leprae-induced leucocyte migration inhibition following lepromin injection in healthy contacts of leprosy. preliminary observations. | 1985 | 3908863 | |
| mouse foot-pad studies with m. leprae--effect of desoxy fructo serotonin (dfs) and related compounds. | mouse foot-pad experiments were carried out to study the effects of dfs and related compounds on the multiplication of m. leprae. of the 25 cases clinically suspected dapsone resistance, 8 were found resistant and 14 sensitive to dapsone by mouse foot-pad experiments. six were resistant to dfs and 16 were sensitive. desoxy fructo 5-hydroxy tryptophane as well as nutrition antileprosy (nal) diet were also found effective in suppressing the growth of m. leprae in mouse foot-pad. of the two liposol ... | 1985 | 3908578 |
| immunity to leprosy. 1. the proliferative response of murine t lymphocytes to mycobacterium leprae. | a microculture assay is described for measuring the response of murine t lymphocytes to mycobacterium leprae antigens. mice were immunized with m. leprae in freund's incomplete adjuvant at the base of the tail, and after five days lymphocytes from draining lymph nodes were harvested and cultured with m. leprae antigens. radioactive thymidine uptake was used to quantitate the antigen-induced proliferation of the lymphocytes. the effects of cell density and antigen concentration on the kinetics of ... | 1985 | 3900249 |
| electron microscopic observations of cell wall and cytoplasmic membrane in murine and human leprosy bacilli. | the cell wall and the cytoplasmic membrane of mycobacterium lepraemurium in murine lepromas and m. leprae in human skin lepromas were studied in ultrathin serial sections at the electron microscopic level. the cell wall in m. lepraemurium was composed of three layers: an innermost electron-dense layer, an intermediate electron-transparent zone, and a thin outermost electron-dense layer. the fine structure of the cell wall in m. leprae was slightly different. in general, the cytoplasmic membrane ... | 1985 | 3900250 |
| use of gas chromatography to differentiate mycobacterium leprae from cultivable armadillo-derived mycobacteria, m. avium/intracellulare, and m. lepraemurium by analysis of secondary alcohols. | two long-chain secondary alcohols, 2-octadecanol and 2-eicosanol, were demonstrated by gas chromatography in hydrolysates of mycobacterium avium/intracellulare, in cultivable, armadillo-derived mycobacteria, and in m. lepraemurium grown in vivo, but they were not found in purified suspensions of m. leprae isolated from experimentally infected armadillos. gas chromatographic analysis of these alcohols constitutes a method for rapid detection and quantification of contaminating mycobacteria in pre ... | 1985 | 3900251 |
| isolation of characteristic glycolipids possibly included in spherical droplets around m. leprae. | the main purpose of this work was to isolate the components in acetone soluble lipids of lepromas of the nine-banded armadillo by high performance liquid chromatography (hplc), and then to examine the mass spectrometric characteristics of the two peaks (molecular weights 2000 and 1600) found by hplc. the armadillo had been inoculated with mycobacterium leprae isolated from a mangabey monkey with naturally acquired leprosy. according to the results of hplc, gas liquid chromatographic and mass spe ... | 1985 | 3900252 |
| incorporation of 32p into the phospholipids of mycobacterium leprae. | 1985 | 3901877 | |
| further characterization including preliminary chemical analysis of antigen mlw1 from mycobacterium leprae. | mlw1, an antigen preparation from mycobacterium leprae previously shown to have a high content of m. leprae antigen no. 7 (ml7), was found to contain the typical cell wall constituents arabinose, galactose and mannose. the fatty acid composition of mlw1 was largely comparable to that of undisrupted cells. the capacity of mlw1 to stimulate lymphocytes was further studied. good correlation was obtained between the in vitro lymphocyte responses to mlw1 and human-derived m. leprae, indicating simila ... | 1985 | 3902662 |
| the free lipids of mycobacterium leprae harvested from experimentally infected nine-banded armadillos. | the free lipids of a sample of mycobacterium leprae were extracted by a procedure designed to produce separate non-polar and polar fractions. the composition of these lipids was analysed semi-quantitatively by five special thin-layer chromatographic systems covering the total range of mycobacterial lipid polarities. in order of increasing polarity, the major lipids were dimycocerosates of phthiocerol a, phthiocerol b and phthiodiolone a, glycosyl phenolphthiocerol dimycocerosates and phospholipi ... | 1985 | 3903039 |
| prevalence of drug resistance in dharmapuri and a. pallipatti areas of tamil nadu. | an evaluation of leprosy control project was undertaken in dharmapuri and a. pallipatti areas of tamil nadu to study the prevalence rate of drug resistance among the leprosy patients. at the end of 5 years of assessment 266 patients were still found to be bacteriologically positive among whom 25 patients were suspected to be clinically dapsone resistant. by mouse foot pad technique the drug resistant prevalence rate was found to be 1.1 per cent in these two areas. | 1985 | 3908580 |
| viability of mycobacterium leprae within the gut of aedes aegypti after they feed on multibacillary lepromatous patients: a study by fluorescent and electron microscopes. | 1985 | 3908862 | |
| quantitative comparison of the mycolic and fatty acid compositions of mycobacterium leprae and mycobacterium gordonae. | the mycolic and fatty acids of three samples each of mycobacterium leprae and mycobacterium gordonae were compared. acids released by whole-organism alkaline hydrolysis were converted to 4-nitrobenzyl esters and mycolic acids were further derivatized to t-butyldimethylsilyl ethers. thin-layer chromatography of the derivatized long-chain extracts showed that all three m. leprae preparations contained so-called alpha-mycolates and ketomycolates but that the m. gordonae samples had a methoxymycolat ... | 1985 | 3903040 |
| the action of deoxyfructose serotonin on intracellular bacilli and on host response in leprosy. | 1985 | 3903407 | |
| serum demyelinating factors and adjuvant-like activity of mycobacterium leprae: possible causes of early nerve damage in leprosy. | 1985 | 3903408 | |
| presence of soluble, mycobacterium leprae-derived antigen in the inflammatory exudate of reactional lepromatous leprosy. | 1985 | 3903409 | |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 49-1985. a 25-year-old haitian man with hansen's disease, painful subcutaneous nodules, fever, and hypesthesia. | 1985 | 3903507 | |
| identification of lymphocyte subsets in leprosin a positive sites following vaccination. | 1985 | 3905743 | |
| transmission of leprosy in nude mice. | nude mice in groups of 10 were exposed to m. leprae by subcutaneous injection and topically through the nose, lungs, mouth, stomach and skin, broken and unbroken. animals injected subcutaneously and those topically exposed to m. leprae through the nose developed localized disease which in the course of time became generalized. the nose seems to be the site of entry of m. leprae in this model. to the extent that these results can be generalized to humans exposed to m. leprae, it would seem that l ... | 1985 | 3914846 |
| [clinical therapy of leprosy and acid-fast bacilli in the nervous system]. | 1985 | 3905745 | |
| mycobacterium leprae-induced alterations in macrophage fc receptor expression and monocyte-lymphocyte interaction in familial contacts of leprosy patients. | macrophage fc receptor expression and monocyte-lymphocyte interaction in the presence of mycobacterium leprae were examined in familial contacts of leprosy patients. defective m phi functions similar to those of borderline and lepromatous patients could be observed in approximately 71% of consanguineous contacts and 43% of spouses of index patients. although the values in the latter group were markedly lower than those of the consanguineous contacts, they tended to be higher than those of normal ... | 1985 | 3906853 |
| vaccines against leprosy. | 1985 | 3907649 | |
| isolation of the mycobacterium leprae-specific glycolipid antigen, phenolic glycolipid-i, from formalin-fixed human lepromatous liver. | a mycobacterium leprae-specific phenolic glycolipid antigen was purified from formalin-fixed liver preserved from an advanced lepromatous leprosy patient. its chemical and immunological properties were compared with those of phenolic glycolipid-i obtained from m. leprae-infected armadillo liver. based on the findings that the glycolipids from the two sources have the same thin-layer chromatographic properties, infrared absorption spectrum, sugar composition, and seroreactivity, we conclude that ... | 1985 | 3908478 |
| immunological potential of a cultivable mycobacterial strain m. habana against leprosy bacillus in mouse foot pad. | a strain of a typical mycobacteria m. habana originally afforded protection against m. tuberculosis challenge in mice, was tested for its immunological potential against leprosy bacillus in the mouse foot pad. the vaccine strain m. habana has arrested the growth of m. leprae into the mouse foot pad better than bcg (phipps) and unvaccinated control. | 1985 | 3908575 |
| [immunological study of the easter island population to detect hansen's infection]. | 1985 | 3915139 | |
| vaccination of leprosy patients and healthy contacts. | 1985 | 3908576 | |
| production of monoclonal antibodies against mycobacterium leprae and armadillo-derived mycobacteria. | six monoclonal antibodies to mycobacterium leprae and armadillo-derived mycobacteria were produced. the monoclonal antibodies were characterized by an immunofluorescence assay using 22 mycobacterial strains. one monoclonal antibody, f47-21-3, reacted only with m. leprae; two, f45-9 and f45-15, reacted only with two armadillo-derived mycobacterial strains. these six monoclonal antibodies can be used for the identification of m. leprae and armadillo-derived mycobacteria. | 1985 | 3909933 |
| leprosy vaccine. large-scale trials begin in india. | 1985 | 3903512 | |
| vaccines against mycobacteria and other intracellular multiplying bacteria. | as the prototype of a vaccine against mycobacterial infection, the bcg (bacille bilié calmette et guérin) has been used against tuberculosis for more than 60 years. it is the only live attenuated vaccine used on humans in more than 182 countries or territories in the world, and very few changes have been made in its fabrication and distribution, except for the production of lyophilized seed-lots. however, its history is marked with controversies concerning its innocuity and efficacy. while bcg s ... | 1985 | 3909929 |
| fixation of mycobacterium leprae in unstained smears prior to shipment or storage: evaluation of formaldehyde using mycobacterium tuberculosis as indicator of efficacy. | 1985 | 3920468 | |
| experimental leprosy in three species of monkeys. | eleven mangabey monkeys inoculated with mycobacterium leprae developed lepromatous-type leprosy. nine of the mangabeys were inoculated with m. leprae isolated from a mangabey with naturally acquired lepromatous leprosy. immune function was depressed in some of these animals after dissemination of the disease. two mangabeys developed lepromatous leprosy after inoculation with human m. leprae passaged in an armadillo. three rhesus and three african green monkeys inoculated with mangabey-derived m. ... | 1985 | 3917577 |
| the allyl group for protection in carbohydrate chemistry. 17. synthesis of propyl o-(3,6-di-o-methyl-beta-d-glucopyranosyl)-(1----4)-o-(2,3- di-o-methyl-alpha-l-rhamnopyranosyl)-(1----2)-3-o-methyl-alpha- l-rhamnopyranoside: the oligosaccharide portion of the major serologically active glycolipid from mycobacterium leprae. | allyl 4-o-benzyl-alpha-l-rhamnopyranoside was converted into allyl 4-o-benzyl-3-o-methyl-alpha-l-rhamnopyranoside and this was condensed with 2,3,4-tri-o-acetyl-alpha-l-rhamnopyranosyl chloride to give a disaccharide derivative which was converted into allyl 4-o-benzyl-2-o-(2,3-o-isopropylidene-alpha-l-rhamnopyranosyl)-3-o-methyl -alpha- l-rhamnopyranoside. this disaccharide derivative was condensed with 2,4-di-o-acetyl-3,6-di-o-methyl-alpha-d-glucopyranosyl chloride to give a trisaccharide deri ... | 1985 | 3910287 |
| detection of an antigen (my4) common to m. tuberculosis and m. leprae by 'tandem' immunoassay. | a novel 'tandem' immunoassay for the detection of mycobacterial antigen was devised using a monoclonal antibody (ml 34) both as solid phase 'capture' and as the 125i- or enzyme-labelled 'tracer' antibody. this antibody binds to the repeating epitopes (my4b) of a water-soluble protease-resistant antigen from m. tuberculosis, m. leprae and some other species of mycobacteria. optimal binding results could be obtained within 4 h by the consecutive incubation of ml34-coated microtitre plates with ant ... | 1985 | 3923119 |
| epidemiology of leprosy in relation to control. report of a who study group. | 1985 | 3925646 | |
| periodic acid-schiff reaction stains mycobacterium tuberculosis, mycobacterium leprae, mycobacterium ulcerans, mycobacterium chelonei (abscessus), and mycobacterium kansasii. | 1985 | 3925927 | |
| characterization of the specificity of monoclonal antibodies against mycobacterium tuberculosis by crossed immunoelectrophoresis. | the specificity of monoclonal antibodies against mycobacterium tuberculosis was determined by crossed immunoelectrophoresis. radiolabelled, non-precipitating monoclonal antibody was incorporated in the top gel together with unlabelled polyvalent, precipitating anti-bacillus calmette-guérin (bcg) antibody. inclusion of labelled antibody in individual precipitate lines was demonstrated by autoradiography. each monoclonal antibody was localized in a single precipitate line: antibody tb73 reacted wi ... | 1985 | 3927476 |
| determination of antibodies in dried blood from earlobes of leprosy patients by enzyme-linked immunosorbent assay--a preliminary report. | an enzyme-linked immunosorbent assay (elisa) was used with soluble antigens of mycobacterium leprae. all blood samples collected from the earlobes of 109 leprosy patients and 100 healthy controls (from a non-endemic area of leprosy) were absorbed with m. vaccae, bcg, cardiolipin, and lecithin according to the technology of the fla-abs test before being tested in the elisa. the results (at a 1:200 blood dilution) showed that antibody activity gradually increased from tt to ll (mean od values: tt ... | 1985 | 3910746 |
| electron microscopic observations of acid-fast bacilli in nasal mucosal biopsies of lepromatous leprosy patients. | the fine structure of acid-fast bacilli in nasal mucosal lepromas of lepromatous leprosy patients was studied in ultrathin sections by the electron microscope. the cell envelope of the bacilli was the main point of these observations. the electron microscopic examination of some of the bacilli revealed the envelope to be multilaminate. some of the cellular matter was filled with an electron-less-dense, amorphous substance, surrounded with a membrane-like structure, and was adherent to the outerm ... | 1985 | 3910747 |
| an experimental study of the antileprosy activity of a series of thioamides in the mouse. | a series of substituted thioamides have been studied to establish whether their structure-activity pattern against mycobacterium leprae is similar to that displayed against m. tuberculosis. antileprosy activity was evaluated in the mouse foot pad using both the kinetic and continuous methods. ethionamide and prothionamide were found to be the most active compounds and to be of approximately equal potency. thioisonicotinamide was about five times less active. 2-t-butyl-thioisonicotinamide, 2-dime ... | 1985 | 3910748 |
| temperature-induced changes in viability, diphenoloxidase and permeability of mycobacterium leprae. | among mycobacteria secretion of the enzyme diphenoloxidase has been established as a property of mycobacterium leprae. the antileprosy drug dapsone (dds), which completely inhibits the enzyme from plant and mammalian sources, does not readily penetrate intact m. leprae. when the drug is complexed with polylysine, it easily permeates the bacteria and produces 100% inhibition of its diphenoloxidase, suggesting a permeability barrier of the cytoplasmic membrane of m. leprae to dapsone. in this stud ... | 1985 | 3929029 |
| experimental reproduction of leprosy in seven-banded armadillos (dasypus hybridus). | four autochthonous seven-banded armadillos (dasypus hybridus) from argentina which developed disseminated leprosy are reported. the average time from inoculation to death was 14.6 months. acid-fast bacilli (afb) were found in the skin, nerves, spleen, lymph nodes, lungs, meninges, and striated muscle. several tests were used to prove that the afb found in the infected animals were mycobacterium leprae: a) failure to grow in standard culture media for mycobacteria, b) extraction of acid-fastness ... | 1985 | 3910749 |
| a possible metabolic role for o-diphenoloxidase in mycobacterium leprae. | among mycobacteria, mycobacterium leprae is unique in its ability to oxidize a variety of diphenols to quinones in vitro. what physiologic role o-diphenoloxidase has in the organism remained unknown. reducing substrates like nadph, nadh and ascorbic acid reacted with the quinone formed from dopa (3,4-dihydroxyphenylalanine); the substrates were oxidized and the quinone was reduced back to diphenol in the process. since the quinone undergoes reversible oxidation-reduction, diphenoloxidase might s ... | 1985 | 3935479 |
| leucocyte migration inhibition response of mycobacterium habana with sensitized animals and cells from leprosy patients. | response of mycobacterium habana sensitized mouse cells against habanin (sonicated extract) lepromin (dharmendra) and tuberculin has been studied in the leucocyte migration inhibition test. the homologous antigenic response has evoked maximum inhibition as compared to heterologous antigens but close antigenic association has also been observed with lepromin and tuberculin. with human cells from leprosy patients these antigens have evoked analogous response indicating cross immunogenicity of m. h ... | 1985 | 3935726 |
| the role of interleukin-2 (il-2) in the specific unresponsiveness of lepromatous leprosy to mycobacterium leprae: studies in vitro and in vivo. | the role of il-2 in the immunological deficiency of lepromatous leprosy patients towards mycobacterium leprae have been studied further. after initial stimulation with m. leprae + il-2, lepromatous lymphocytes could be restimulated with m. leprae alone. the specificity of the responses obtained varied. some patients gave a stronger response to bcg as compared to m. leprae, while in others a stronger response to m. leprae as compared to bcg was obtained. studies of the composition of lymphocytes ... | 1985 | 3936783 |
| monoclonal antibodies and recombinant dna technology: present and future uses in leprosy and tuberculosis. | 1985 | 3910750 | |
| distribution of mycobacterium leprae in the circles of counting slides. | 1985 | 3910751 | |
| [lysosome and leprosy bacilli]. | 1985 | 3939304 | |
| cross-reactivity of antigens from the cytoplasm and cell walls of some corynebacteria and mycobacteria. | leprosy-derived corynebacteria (ldc) are non-acid-fast organisms isolated from leprosy lesions in humans. in this study 20 antigens of native ldc cytoplasm were identified by immunoelectrophoresis, and autoclaving yielded the m1 component, which strongly cross-reacted with antigen 60 of mycobacterium bovis bcg (bacille calmette-guérin) and antigen 7 of mycobacterium leprae. the polysaccharide moiety of m1 was immunologically related to the ldc cell wall polysaccharide previously characterized as ... | 1985 | 3965589 |
| distribution of mycobacterium leprae in circular counting films. | 1985 | 3910752 | |
| recent trends in chemotherapy of leprosy. | 1985 | 3912375 | |
| the lack of therapeutic effects in mice of the combined gamma-irradiated mycobacterium leprae and viable bcg against mycobacterium leprae infection. | 1985 | 3913661 | |
| does the hansen bacillus harm or benefit the leprous patient? | 1985 | 3913662 | |
| measurement of atp generation and decay in mycobacterium leprae in vitro. | the intracellular atp content of mycobacterium leprae isolated from armadillo tissue was approximately 1.5 x 10(-16) g per bacillus. during in vitro incubation of bacilli at 4 degrees c, 33 degrees c or 37 degrees c there was an exponential decrease in atp content, the rate depending on the medium and the temperature. m. leprae incorporated phosphate into atp and into other nucleotide materials during in vitro incubation. | 1985 | 3913745 |
| hla class-ii immune response genes and products in leprosy. | 1985 | 3918314 | |
| histology of the mitsuda reaction of healthy adults with no known contacts with leprosy patients. | mitsuda tests were performed on 100 healthy adult brazilian males unrelated consanguineously to leprosy patients and with no known contacts with this disease. three lepromatous patients served as negative mitsuda controls. the lepromin employed was from mycobacterium leprae-infected armadillo tissue prepared according to who standards. clinical response to the lepromin inoculation was graded according to the recommendations of the vi international leprosy congress held in madrid (1953). the hist ... | 1985 | 4086917 |
| effect of repeated lepromin testing on experimental nine-banded armadillo leprosy. | twenty-eight armadillos were lepromin tested and infected with m. leprae; 18 intravenously and 10 intradermally. the lepromin test was repeated after 3 months and at intervals of 6 months thereafter until their death or sacrifice up to 30 months. the one animal with tuberculoid lepromin was resistant and 14 of the 16 with lepromatous lepromin developed generalized disease. of the 11 with borderline lepromin, 6 developed disseminated disease and 5 were resistant. there is a definite relationship ... | 1985 | 3915005 |
| [diagnostic test systems based on the immunoenzyme method in leprosy]. | diagnostic test systems for the detection of igg and igm to mycobacterium leprae in the blood sera of leprosy patients and armadillos experimentally infected with m. leprae have been developed on the basis of the indirect immunoperoxidase assay. the possibility has been shown of prognosing the activity of the leprotic process in leprosy patients and the results of the experimental infection of armadillos by the dynamic increase of antibody reactions with the development of the infection. | 1985 | 3885647 |
| natural emergence of antigen-reactive t cells in lepromatous leprosy patients during erythema nodosum leprosum. | fifteen lepromatous leprosy (ll) patients undergoing erythema nodosum leprosum (enl) reactions were compared with 13 stable, uncomplicated, anergic individuals of the same leprosy background. enl patients showed significant antigen-induced leukocyte migration inhibition (migration index = 0.058 +/- 0.01), paralleling the values obtained with a responder tuberculoid leprosy population (migration index = 0.04 +/- 0.004). both phytohemagglutinin-induced general t-cell proliferation and, more signif ... | 1985 | 2933339 |
| host-parasite interaction in relation to leprosy. | 1985 | 2934487 | |
| cellular immunity in lepromatous and tuberculoid leprosy. | the depression of cellular immunity in lepromatous patients is not understood. while the blood monocytes of leprosy patients appear to be activated normally by lymphokines, t cell proliferation and production of lymphokines in response to mycobacterium leprae are impaired in lepromatous patients. attempts to restore responsiveness in cells from these patients have been unsuccessful in our hands. the addition of exogenous il-2 to leukocyte cultures does not appear to restore responsiveness to m. ... | 1985 | 2935494 |
| interleukin-2-induced t-cell response to m. leprae in lepromatous leprosy: reversion of a suppressor mechanism or expansion of a small m. leprae-reactive t-cell pool? | 1985 | 2935587 | |
| [leprous symmetric keratoderma palmaris et plantaris. apropos of 2 clinical cases]. | two cases of "keratoderma palmaris et plantaris symmetric leprous" are described, which rarely occur in the field of acrokeratosis, contracted by infection. | 1985 | 2977127 |
| hydrogen peroxide and superoxide production by peripheral blood monocytes in leprosy. | susceptibility to infection with mycobacterium leprae, the causative organism of leprosy, is the result of a defect in cell-mediated immunity (cmi). the co-operation of macrophages and t lymphocytes is known to be essential for competent cmi response. in this study we have examined peripheral blood monocytes from a range of leprosy patients in an attempt to identify a possible defect in macrophage function. the ability of these cells to produce hydrogen peroxide and superoxide, two bactericidal ... | 1985 | 2988833 |
| human monoclonal antibodies against mycobacterium leprae. | human hybridomas were constructed which produce antibodies against three different extracts of mycobacterium leprae. a thioguanine-resistant (thgr), ouabain-resistant (ouar), human lymphoblastoid cell line, kr-4, was hybridized with epstein-barr virus-transformed cell lines from lepromatous leprosy patients with fusion frequencies of greater than 10(-5). non-epstein-barr virus-transformed donor cells fused at much lower rates (less than 2 x 10(-7]. hybrids were selected in medium containing hypo ... | 1985 | 2989177 |
| activation of human monocytes in leprosy. | in leprosy, the common etiologic agent is the same mycobacterium leprae, but the clinical manifestations are various, including the tuberculoid and lepromatous types. in tuberculoid type leprosy, macrophages in the granuloma differentiate into epithelioid cells; in the lepromatous type, in contrast, they differentiate into lepra cells containing multiple m. leprae. thus host factors, which regulate macrophage activities, determine the type of leprosy. to understand such regulation of macrophage ... | 1985 | 2989662 |
| evidence for the activity of rifampin on the neuropathy of foot pad-inoculated mice with mycobacterium leprae. | 1985 | 2995517 | |
| epstein-barr virus-transformed b cell lines present m. leprae antigens to t cells. | we have investigated whether or not epstein-barr virus-transformed lymphoblastoid b cell lines (ebv-blcl) are able to present mycobacterium leprae (m. leprae) to antigen-reactive t cell lines and clones. such ebv-blcl would provide us with a homogeneous and unlimited source of antigen-presenting cells. antigen-triggered proliferation of t cells has been studied with co-cultures either with autologous or allogeneic irradiated ebv-blcl. our results show that ebv-blcl are able to present m. leprae ... | 1985 | 3001928 |
| ultrastructure of leprous phlebitis. | an ultrastructural study on vein biopsies from six lepromatous leprosy patients was carried out. the results showed that a) the lumen-lining bacillated cells were endothelial in origin due to the presence of specific weibel-palade endothelial cell granules; b) endothelial cells released mycobacterium leprae into the lumen by exophagocytosis; c) m. leprae were able to grow and multiply in the endothelial and smooth muscle cells; and d) smooth muscle cells did not show any evidence of reaction due ... | 1985 | 3003213 |
| respiration in mycobacterium leprae. | fairly pure leprosy bacilli were easily collected from nude mouse foot pad lepromas by the ficoll density gradient centrifugation and alkali treatment methods. the yield of bacilli available for biochemical study was 42.6%. the density of mycobacterium leprae was very heterogeneous. the percent of solid bacilli in the light bacilli fraction was 23%; that in the heavy bacilli fraction was 40%. the endogenous respiration activity in the heavy bacilli was greater than that in light bacilli. the ave ... | 1985 | 3003214 |
| defective cell-mediated immunity in leprosy: failure of t cells from lepromatous leprosy patients to respond to mycobacterium leprae is associated with defective expression of interleukin 2 receptors and is not reconstituted by interleukin 2. | patients with lepromatous leprosy (ll) but not borderline tuberculoid leprosy (bt) have defective cell-mediated immune responses to mycobacterium leprae, despite normal responses to other stimuli, as judged by in vivo skin testing and in vitro lymphocyte transformation. to investigate the basis of the immune defect in ll patients, we studied the ability of patient mononuclear leukocytes to produce interleukin 1 (il 1) and interleukin 2 (il 2) upon stimulation with m. leprae, and determined the a ... | 1985 | 2989366 |
| resistance to mycobacteria in mice treated with fractionated total lymphoid irradiation (tli) and in mice reconstituted with allogenic bone marrow cells following radiotherapy. | the increased clinical use of total lymphoid irradiation (tli) as an immunosuppressive adjunct in transplantation suggested the need for determining the effects of tli on the in vivo susceptibility of animals to infections controlled by cell-mediated immunity. tli-treated, tli-treated and splenectomized, and chimeric mice prepared with tli were inoculated in the hind foot pad with mycobacterium marinum or mycobacterium leprae. although m. marinum organisms multiplied in greater numbers in the tl ... | 1985 | 3155713 |
| immunoassays of field isolates of mycobacterium bovis and other mycobacteria by use of monoclonal antibodies. | antigen extracts obtained by sonication of 22 strains of mycobacterium bovis from cattle and badgers together with extracts of strains of m. tuberculosis, m. paratuberculosis, m. avium, m. africanum, m. kansasi, m. leprae and bcg were examined with a panel of 10 monoclonal antibodies to m. tuberculosis or m. leprae. antigen extracts were coated in aqueous solution (wet coating) and the extracts were also dried on to the polyvinyl plates (dry coating). when dry coating was compared to wet coating ... | 1985 | 2409287 |
| monoclonal antibodies to a 28,000 mol. wt protein antigen of mycobacterium leprae. | monoclonal antibodies (moab) have been used to analyse a protein antigen from mycobacterium leprae with a subunit mol. wt of 28,000 daltons. three different patterns of species specificity were observed with two antibodies being specific for m. leprae, two partially specific, and one broadly cross-reactive amongst all mycobacteria. competitive binding and sandwich assays demonstrated that the specific and partially specific antibodies recognized closely related regions of the molecule while the ... | 1985 | 2410167 |
| multi-drug therapy of leprosy. | 1985 | 2420903 | |
| quality control of skin smear services in leprosy programmes: preliminary experience with inter-observer comparison in routine services. | 1985 | 2414625 |