Publications

construction of synthetic promoter-based expression cassettes for the production of cadaverine in recombinant corynebacterium glutamicum.	corynebacterium glutamicum is an important microorganism in the biochemical industry for the production of various platform chemicals. however, despite its importance, a limited number of studies have been conducted on how to constitute gene expression cassettes in engineered c. glutamicum to obtain desired amounts of the target products. therefore, in this study, six expression cassettes for the expression of the second lysine decarboxylase of escherichia coli, ldcc, were constructed using six ...	2015	26047931
fermentative production of the diamine putrescine: system metabolic engineering of corynebacterium glutamicum.	corynebacterium glutamicum shows great potential for the production of the glutamate-derived diamine putrescine, a monomeric compound of polyamides. a genome-scale stoichiometric model of a c. glutamicum strain with reduced ornithine transcarbamoylase activity, derepressed arginine biosynthesis, and an anabolic plasmid-addiction system for heterologous expression of e. coli ornithine decarboxylase gene spec was investigated by flux balance analysis with respect to its putrescine production poten ...	2015	25919117
development of an orthogonal fatty acid biosynthesis system in e. coli for oleochemical production.	here we report recombinant expression and activity of several type i fatty acid synthases that can function in parallel with the native escherichia coli fatty acid synthase. corynebacterium glutamicum fas1a was the most active in e. coli and this fatty acid synthase was leveraged to produce oleochemicals including fatty alcohols and methyl ketones. coexpression of fas1a with the acp/coa-reductase maqu2220 from marinobacter aquaeolei shifted the chain length distribution of fatty alcohols produce ...	2015	25887638
enhanced production of gamma-aminobutyrate (gaba) in recombinant corynebacterium glutamicum by expressing glutamate decarboxylase active in expanded ph range.	gamma-aminobutylate (gaba) is an important chemical in pharmacetucal field and chemical industry. gaba has mostly been produced in lactic acid bacteria by adding l-glutamate to the culture medium since l-glutamate can be converted into gaba by inherent l-glutamate decarboxylase. recently, gaba has gained much attention for the application as a major building block for the synthesis of 2-pyrrolidone and biodegradable polyamide nylon 4, which opens its application area in the industrial biotechnol ...	2015	25886194
regulation of expression of soda and msra genes of corynebacterium glutamicum in response to oxidative and radiative stress.	promoters of genes encoding superoxide dismutase (soda) and peptide methionine sulfoxide reductase (msra) from cory-nebacterium glutamicum were cloned and sequenced. promoter region analysis of soda-msra was unable to identify putative sites of fixed eventual regulators except for possible sites of fixed oxyr and integra-tion host factor. a study of the regulation of these genes was performed using the lacz gene of escherichia coli as a reporter placed under the control of sequences downstream o ...	2015	25867357
characterization of a flavin-containing monooxygenase from corynebacterium glutamicum and its application to production of indigo and indirubin.	to examine the role of a gene encoding flavin-containing monooxygenase (cfmo) from corynebacterium glutamicum atcc13032 when cloned and expressed in escherichia coli for the production of indigo pigments.	2015	25851950
diaminopimelic acid amidation in corynebacteriales: new insights into the role of ltsa in peptidoglycan modification.	a gene named ltsa was earlier identified in rhodococcus and corynebacterium species while screening for mutations leading to increased cell susceptibility to lysozyme. the encoded protein belonged to a huge family of glutamine amidotransferases whose members catalyze amide nitrogen transfer from glutamine to various specific acceptor substrates. we here describe detailed physiological and biochemical investigations demonstrating the specific role of ltsa protein from corynebacterium glutamicum ( ...	2015	25847251
distinct paths for basic amino acid export in escherichia coli: ybje (lyso) mediates export of l-lysine.	in escherichia coli, argo encodes an exporter for l-arginine (arg) and its toxic analogue canavanine (can), and its transcriptional activation and repression, by arg and l-lysine (lys), respectively, are mediated by the regulator argp. accordingly argo and argp mutants are can supersensitive (can(ss)). we report the identification of ybje as a gene encoding a predicted inner membrane protein that mediates export of lys, and our results confirm the previous identification with a different approac ...	2015	25845847
identification of essential tryptophan in amylomaltase from corynebacterium glutamicum.	this work aims to identify essential tryptophan residue(s) of amylomaltase from corynebacterium glutamicum (cgam) through chemical modification and site-directed mutagenesis techniques. the recombinant enzyme expressed by escherichia coli was purified and treated with n-bromosuccinimide (nbs), a modifying agent for tryptophan. a significant decrease in enzyme activity was observed indicating that tryptophan is important for catalysis. inactivation kinetics with nbs resulted in pseudo first-order ...	2015	25748841
high-level production of bacillus cereus phospholipase c in corynebacterium glutamicum.	enzymatic oil degumming (removal of phospholipids) using phospholipase c (plc) is a well-established and environmentally friendly process for vegetable oil refining. in this work, we report the production of recombinant bacillus cereus plc in corynebacterium glutamicum atcc 13869 in a high cell density fermentation process and its performance in soybean oil degumming. a final concentration of 5.5g/l of the recombinant enzyme was achieved when the respective gene was expressed from the tac promot ...	2015	26519562
[characterization of l-aspartate-α-decarboxylase from bacillus subtilis].	as an important material in pharmaceutical and chemical industry, β-alanine was mainly produced by chemical methods. l-aspartate-α-decarboxylase could catalyze the α-decarboxylation from l-aspartate to β-alanine. determinations for specific activities of pands from escherichia coli, corynebacterium glutamicum and bacillus subtilis were performed in this study (0.98 u/mg, 7.52 u/mg and 8.4 u/mg respectively). the optimal temperature and ph of pands from c. glutamicum and b. subtilis were 65 degre ...	2015	26762040
a tatabc-type tat translocase is required for unimpaired aerobic growth of corynebacterium glutamicum atcc13032.	the twin-arginine translocation (tat) system transports folded proteins across the cytoplasmic membrane of bacteria and the thylakoid membrane of plant chloroplasts. escherichia coli and other gram-negative bacteria possess a tatabc-type tat translocase in which each of the three inner membrane proteins tata, tatb, and tatc performs a mechanistically distinct function. in contrast, low-gc gram-positive bacteria, such as bacillus subtilis, use a tatac-type minimal tat translocase in which the tat ...	2015	25837592
exploring lysine riboswitch for metabolic flux control and improvement of l-lysine synthesis in corynebacterium glutamicum.	riboswitch, a regulatory part of an mrna molecule that can specifically bind a metabolite and regulate gene expression, is attractive for engineering biological systems, especially for the control of metabolic fluxes in industrial microorganisms. here, we demonstrate the use of lysine riboswitch and intracellular l-lysine as a signal to control the competing but essential metabolic by-pathways of lysine biosynthesis. to this end, we first examined the natural lysine riboswitches of eschericia co ...	2015	25575181
metabolic engineering of corynebacterium glutamicum for the production of itaconate.	the capability of corynebacterium glutamicum for glucose-based synthesis of itaconate was explored, which can serve as building block for production of polymers, chemicals, and fuels. c. glutamicum was highly tolerant to itaconate and did not metabolize it. expression of the aspergillus terreus cad1 gene encoding cis-aconitate decarboxylase (cad) in strain atcc13032 led to the production of 1.4mm itaconate in the stationary growth phase. fusion of cad with the escherichia coli maltose-binding pr ...	2015	26100077
metabolic engineering of itaconate production in escherichia coli.	interest in sustainable development has led to efforts to replace petrochemical-based monomers with biomass-based ones. itaconic acid, a c5-dicarboxylic acid, is a potential monomer for the chemical industry with many prospective applications. cis-aconitate decarboxylase (cada) is the key enzyme of itaconate production, converting the citric acid cycle intermediate cis-aconitate into itaconate. heterologous expression of cada from aspergillus terreus in escherichia coli resulted in low cada acti ...	2015	25277412
corynebacterium glutamicum methionine sulfoxide reductase a uses both mycoredoxin and thioredoxin for regeneration and oxidative stress resistance.	oxidation of methionine leads to the formation of the s and r diastereomers of methionine sulfoxide (meto), which can be reversed by the actions of two structurally unrelated classes of methionine sulfoxide reductase (msr), msra and msrb, respectively. although msras have long been demonstrated in numerous bacteria, their physiological and biochemical functions remain largely unknown in actinomycetes. here, we report that a corynebacterium glutamicum methionine sulfoxide reductase a (cgmsra) tha ...	2015	25681179
engineering corynebacterium glutamicum for the production of 2,3-butanediol.	2,3-butanediol is an important bulk chemical with a wide range of applications. in bacteria, this metabolite is synthesised from pyruvate via a three-step pathway involving α-acetolactate synthase, α-acetolactate decarboxylase and 2,3-butanediol dehydrogenase. thus far, the best producers of 2,3-butanediol are pathogenic strains, hence, the development of more suitable organisms for industrial scale fermentation is needed. herein, 2,3-butanediol production was engineered in the generally regarde ...	2015	26511723
metabolic pathway engineering for production of 1,2-propanediol and 1-propanol by corynebacterium glutamicum.	production of the versatile bulk chemical 1,2-propanediol and the potential biofuel 1-propanol is still dependent on petroleum, but some approaches to establish bio-based production from renewable feed stocks and to avoid toxic intermediates have been described. the biotechnological workhorse corynebacterium glutamicum has also been shown to be able to overproduce 1,2-propanediol by metabolic engineering. additionally, c. glutamicum has previously been engineered for production of the biofuels e ...	2015	26110019
screening currency notes for microbial pathogens and antibiotic resistance genes using a shotgun metagenomic approach.	fomites are a well-known source of microbial infections and previous studies have provided insights into the sojourning microbiome of fomites from various sources. paper currency notes are one of the most commonly exchanged objects and its potential to transmit pathogenic organisms has been well recognized. approaches to identify the microbiome associated with paper currency notes have been largely limited to culture dependent approaches. subsequent studies portrayed the use of 16s ribosomal rna ...	2015	26035208
single-domain peptidyl-prolyl cis/trans isomerase fkpa from corynebacterium glutamicum improves the biomass yield at increased growth temperatures.	peptidyl-prolyl cis/trans isomerases (ppiases) catalyze the rate-limiting protein folding step at peptidyl bonds preceding proline residues and were found to be involved in several biological processes, including gene expression, signal transduction, and protein secretion. representative enzymes were found in almost all sequenced genomes, including corynebacterium glutamicum, a facultative anaerobic gram-positive and industrial workhorse for the production of amino acids. in c. glutamicum, a pre ...	2015	26341203
a prophage-encoded actin-like protein required for efficient viral dna replication in bacteria.	in host cells, viral replication is localized at specific subcellular sites. viruses that infect eukaryotic and prokaryotic cells often use host-derived cytoskeletal structures, such as the actin skeleton, for intracellular positioning. here, we describe that a prophage, cgp3, integrated into the genome of corynebacterium glutamicum encodes an actin-like protein, alpc. biochemical characterization confirms that alpc is a bona fide actin-like protein and cell biological analysis shows that alpc f ...	2015	25916847
identification of two mutations increasing the methanol tolerance of corynebacterium glutamicum.	methanol is present in most ecosystems and may also occur in industrial applications, e.g. as an impurity of carbon sources such as technical glycerol. methanol often inhibits growth of bacteria, thus, methanol tolerance may limit fermentative production processes.	2015	26474849
engineering the glycolytic pathway: a potential approach for improvement of biocatalyst performance.	the glycolytic pathway is a main driving force in the fermentation process as it produces energy, cell component precursors, and fermentation products. given its importance, the glycolytic pathway can be considered as an attractive target for the metabolic engineering of industrial microorganisms. however, many attempts to enhance glycolytic flux, by overexpressing homologous or heterologous genes encoding glycolytic enzymes, have been unsuccessful. in contrast, significant enhancement in glycol ...	2015	26513591
metabolic engineering of an atp-neutral embden-meyerhof-parnas pathway in corynebacterium glutamicum: growth restoration by an adaptive point mutation in nadh dehydrogenase.	corynebacterium glutamicum uses the embden-meyerhof-parnas pathway of glycolysis and gains 2 mol of atp per mol of glucose by substrate-level phosphorylation (slp). to engineer glycolysis without net atp formation by slp, endogenous phosphorylating nad-dependent glyceraldehyde-3-phosphate dehydrogenase (gapdh) was replaced by nonphosphorylating nadp-dependent glyceraldehyde-3-phosphate dehydrogenase (gapn) from clostridium acetobutylicum, which irreversibly converts glyceraldehyde-3-phosphate (g ...	2015	25576602
metabolic engineering of corynebacterium glutamicum for methanol metabolism.	methanol is already an important carbon feedstock in the chemical industry, but it has found only limited application in biotechnological production processes. this can be mostly attributed to the inability of most microbial platform organisms to utilize methanol as a carbon and energy source. with the aim to turn methanol into a suitable feedstock for microbial production processes, we engineered the industrially important but nonmethylotrophic bacterium corynebacterium glutamicum toward the ut ...	2015	25595770
functional characterization of a vanillin dehydrogenase in corynebacterium glutamicum.	vanillin dehydrogenase (vdh) is a crucial enzyme involved in the degradation of lignin-derived aromatic compounds. herein, the vdh from corynebacterium glutamicum was characterized. the relative molecular mass (mr) determined by sds-page was ~51 kda, whereas the apparent native mr values revealed by gel filtration chromatography were 49.5, 92.3, 159.0 and 199.2 kda, indicating the presence of dimeric, trimeric and tetrameric forms. moreover, the enzyme showed its highest level of activity toward ...	2015	25622822
ohr protects corynebacterium glutamicum against organic hydroperoxide induced oxidative stress.	ohr, a bacterial protein encoded by the organic hydroperoxide resistance (ohr) gene, plays a critical role in resistance to organic hydroperoxides. in the present study, we show that the cys-based thiol-dependent ohr of corynebacterium glutamicum decomposes organic hydroperoxides more efficiently than hydrogen peroxide. replacement of either of the two cys residues of ohr by a ser residue resulted in drastic loss of activity. the electron donors supporting regeneration of the peroxidase activity ...	2015	26121694
biosynthesis of l-sorbose and l-psicose based on c-c bond formation catalyzed by aldolases in an engineered corynebacterium glutamicum strain.	the property of loose stereochemical control at aldol products from aldolases helped to synthesize multiple polyhydroxylated compounds with nonnatural stereoconfiguration. in this study, we discovered for the first time that some fructose 1,6-diphosphate aldolases (frua) and tagatose 1,6-diphosphate (taga) aldolases lost their strict stereoselectivity when using l-glyceraldehyde and synthesized not only l-sorbose but also a high proportion of l-psicose. among the aldolases tested, taga from baci ...	2015	25888171
reactions of cg10062, a cis-3-chloroacrylic acid dehalogenase homologue, with acetylene and allene substrates: evidence for a hydration-dependent decarboxylation.	cg10062 is a cis-3-chloroacrylic acid dehalogenase (cis-caad) homologue from corynebacterium glutamicum with an unknown function and an uninformative genomic context. it shares 53% pairwise sequence similarity with cis-caad including the six active site amino acids (pro-1, his-28, arg-70, arg-73, tyr-103, and glu-114) that are critical for cis-caad activity. however, cg10062 is a poor cis-caad: it lacks catalytic efficiency and isomer specificity. two acetylene compounds (propiolate and 2-butyno ...	2015	25894805
unraveling the kinetic diversity of microbial 3-dehydroquinate dehydratases of shikimate pathway.	3-dehydroquinate dehydratase (dhqase) catalyzes the conversion of 3-dehydroquinic acid to 3-dehydroshikimic acid of the shikimate pathway. in this study, 3180 prokaryotic genomes were examined and 459 dhqase sequences were retrieved. based on sequence analysis and their original hosts, 38 dhqase genes were selected for chemical synthesis. the selected dhqases were translated into new dna sequences according to the genetic codon usage bias by both escherichia coli and corynebacterium glutamicum. ...	2015	25852984
redox regulation by reversible protein s-thiolation in bacteria.	low molecular weight (lmw) thiols function as thiol-redox buffers to maintain the reduced state of the cytoplasm. the best studied lmw thiol is the tripeptide glutathione (gsh) present in all eukaryotes and gram-negative bacteria. firmicutes bacteria, including bacillus and staphylococcus species utilize the redox buffer bacillithiol (bsh) while actinomycetes produce the related redox buffer mycothiol (msh). in eukaryotes, proteins are post-translationally modified to s-glutathionylated proteins ...	2015	25852656
interaction sites of diviva and roda from corynebacterium glutamicum.	elongation growth in actinobacteria is localized at the cell poles. this is in contrast to many classical model organisms where insertion of new cell wall material is localized around the lateral site. we previously described a role of roda from corynebacterium glutamicum in apical cell growth and morphogenesis. deletion of roda had drastic effects on morphology and growth, likely a result from misregulation of penicillin-binding proteins and cell wall precursor delivery. we identified the inter ...	2015	25709601
metabolic engineering of corynebacterium glutamicum for enhanced production of 5-aminovaleric acid.	5-aminovaleric acid (5ava) is an important five-carbon platform chemical that can be used for the synthesis of polymers and other chemicals of industrial interest. enzymatic conversion of l-lysine to 5ava has been achieved by employing lysine 2-monooxygenase encoded by the davb gene and 5-aminovaleramidase encoded by the dava gene. additionally, a recombinant escherichia coli strain expressing the davb and dava genes has been developed for bioconversion of l-lysine to 5ava. to use glucose and xy ...	2016	27717386
systems metabolic engineering of corynebacterium glutamicum for the production of the carbon-5 platform chemicals 5-aminovalerate and glutarate.	the steadily growing world population and our ever luxurious life style, along with the simultaneously decreasing fossil resources has confronted modern society with the issue and need of finding renewable routes to accommodate for our demands. shifting the production pipeline from raw oil to biomass requires efficient processes for numerous platform chemicals being produced with high yield, high titer and high productivity.	2016	27618862
structural insights into a novel class of aspartate aminotransferase from corynebacterium glutamicum.	aspartate aminotransferase from corynebacterium glutamicum (cgaspat) is a plp-dependent enzyme that catalyzes the production of l-aspartate and α-ketoglutarate from l-glutamate and oxaloacetate in l-lysine biosynthesis. in order to understand the molecular mechanism of cgaspat and compare it with those of other aspartate aminotransferases (aspats) from the aminotransferase class i, we determined the crystal structure of cgaspat. cgaspat functions as a dimer, and the cgaspat monomer consists of t ...	2016	27355211
regulation of coenzyme a biosynthesis in the hyperthermophilic bacterium thermotoga maritima.	regulation of coenzyme a (coa) biosynthesis in bacteria and eukaryotes occurs through feedback inhibition targeting type i and type ii pantothenate kinase (pank), respectively. in contrast, the activity of type iii pank is not affected by coa. as the hyperthermophilic bacterium thermotoga maritima harbors only a single type iii pank (tm-pank), here we examined the mechanisms that regulate coa biosynthesis in this organism. we first examined the enzyme responsible for the ketopantoate reductase ( ...	2016	27161115
rapid electron transfer within the iii-iv supercomplex in corynebacterium glutamicum.	complex iii in c. glutamicum has an unusual di-heme cyt. c1 and it co-purifies with complex iv in a supercomplex. here, we investigated the kinetics of electron transfer within this supercomplex and in the cyt. aa3 alone (cyt. bc1 was removed genetically). in the reaction of the reduced cyt. aa3 with o2, we identified the same sequence of events as with other a-type oxidases. however, even though this reaction is associated with proton uptake, no ph dependence was observed in the kinetics. for t ...	2016	27682138
fast mechanically driven daughter cell separation is widespread in actinobacteria.	dividing cells of the coccoid gram-positive bacterium staphylococcus aureus undergo extremely rapid (millisecond) daughter cell separation (dcs) driven by mechanical crack propagation, a strategy that is very distinct from the gradual, enzymatically driven cell wall remodeling process that has been well described in several rod-shaped model bacteria. to determine if other bacteria, especially those in the same phylum (firmicutes) or with similar coccoid shapes as s. aureus, might use a similar m ...	2016	27578753
transcriptome and gene ontology (go) enrichment analysis reveals genes involved in biotin metabolism that affect l-lysine production in corynebacterium glutamicum.	corynebacterium glutamicum is widely used for amino acid production. in the present study, 543 genes showed a significant change in their mrna expression levels in l-lysine-producing c. glutamicum atcc21300 than that in the wild-type c. glutamicum atcc13032. among these 543 differentially expressed genes (degs), 28 genes were up- or downregulated. in addition, 454 degs were functionally enriched and categorized based on blast sequence homologies and gene ontology (go) annotations using the blast ...	2016	27005618
engineering corynebacterium glutamicum for violacein hyper production.	corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to corynebacterium's gras status and advantages in tryptophan fermentation. the violacein is a commercially potential compound with various applications derived from l-tryptophan.	2016	27557730
engineering of corynebacterium glutamicum for xylitol production from lignocellulosic pentose sugars.	xylitol is a non-fermentable sugar alcohol used as sweetener. corynebacterium glutamicum atcc13032 was metabolically engineered for xylitol production from the lignocellulosic pentose sugars xylose and arabinose. direct conversion of xylose to xylitol was achieved through the heterologous expression of nad(p)h-dependent xylose reductase (xr) gene from rhodotorula mucilaginosa. xylitol synthesis from arabinose was attained through polycistronic expression of l-arabinose isomerase (araa), d-psicos ...	2016	27184428
tatc-dependent translocation of pyoverdine is responsible for the microbial growth suppression.	infections are often not caused by a colonization of pseudomonas aeruginosa alone but by a consortium of other bacteria. little is known about the impact of p. aeruginosa on the growth of other bacteria upon coinfection. here, cell-ree culture supernatants obtained from p. aeruginosa suppressed the growth of a number of bacterial strains such as corynebacterium glutamicum, bacillus subtilis, staphylococcus aureus, and agrobacterium tumefaciens, but had little effect on the growth of escherichia ...	2016	26832668
production of the marine carotenoid astaxanthin by metabolically engineered corynebacterium glutamicum.	astaxanthin, a red c40 carotenoid, is one of the most abundant marine carotenoids. it is currently used as a food and feed additive in a hundred-ton scale and is furthermore an attractive component for pharmaceutical and cosmetic applications with antioxidant activities. corynebacterium glutamicum, which naturally synthesizes the yellow c50 carotenoid decaprenoxanthin, is an industrially relevant microorganism used in the million-ton amino acid production. in this work, engineering of a genome-r ...	2016	27376307
use of a sec signal peptide library from bacillus subtilis for the optimization of cutinase secretion in corynebacterium glutamicum.	technical bulk enzymes represent a huge market, and the extracellular production of such enzymes is favorable due to lowered cost for product recovery. protein secretion can be achieved via general secretion (sec) pathway. specific sequences, signal peptides (sps), are necessary to direct the target protein into the translocation machinery. for example, >150 sec-specific sps have been identified for bacillus subtilis alone. as the best sp for a target protein of choice cannot be predicted a prio ...	2016	27927208
engineering corynebacterium glutamicum for fast production of l-lysine and l-pipecolic acid.	the gram-positive corynebacterium glutamicum is widely used for fermentative production of amino acids. the world production of l-lysine has surpassed 2 million tons per year. glucose uptake and phosphorylation by c. glutamicum mainly occur by the phosphotransferase system (pts) and to lesser extent by inositol permeases and glucokinases. heterologous expression of the genes for the high-affinity glucose permease from streptomyces coelicolor and bacillus subtilis glucokinase fully compensated fo ...	2016	27345060
identification of d-amino acid dehydrogenase as an upstream regulator of the autoinduction of a putative acyltransferase in corynebacterium glutamicum.	expression of a putative acyltransferase encoded by ncgl- 0350 of corynebacterium glutamicum is induced by cell-free culture fluids obtained from stationary-phase growth of both c. glutamicum and pseudomonas aeruginosa, providing evidence for interspecies communication. here, we further confirmed that such communication occurs by showing that acyltransferase expression is induced by culture fluid obtained from diverse gram-negative and -positive bacterial strains, including escherichia coli, sal ...	2016	27225460
diverse effects of a biosurfactant from rhodococcus ruber iegm 231 on the adhesion of resting and growing bacteria to polystyrene.	this study evaluated the effects of a trehalolipid biosurfactant produced by rhodococcus ruber iegm 231 on the bacterial adhesion and biofilm formation on the surface of polystyrene microplates. the adhesion of gram-positive (arthrobacter simplex, bacillus subtilis, brevibacterium linens, corynebacterium glutamicum, micrococcus luteus) and gram-negative (escherichia coli, pseudomonas fluorescencens) bacteria correlated differently with the cell hydrophobicity and surface charge. in particular, e ...	2016	26888203
expression of recombinant protein using corynebacterium glutamicum: progress, challenges and applications.	corynebacterium glutamicum (c. glutamicum) is a highly promising alternative prokaryotic host for recombinant protein expression, as it possesses several significant advantages over escherichia coli (e. coli), the currently leading bacterial protein expression system. during the past decades, several experimental techniques and vector components for genetic manipulation of c. glutamicum have been developed and validated, including strong promoters for tightly regulating target gene expression, v ...	2016	25714007
impact of lytr-cpsa-psr proteins on cell wall biosynthesis in corynebacterium glutamicum.	proteins of the lcp (lytr, cpsa, psr) family have been shown to inherit important roles in bacterial cell wall biosynthesis. however, their exact function in the formation of the complex cell wall structures of the corynebacteriales, including the prominent pathogens mycobacterium tuberculosis and corynebacterium diphtheriae, remains unclear. here, we analyzed the role of the lcp proteins lcpa and lcpb of corynebacterium glutamicum, both of which localize at regions of nascent cell wall biosynth ...	2016	27551018
caenorhabditis elegans star formation and negative chemotaxis induced by infection with corynebacteria.	caenorhabditis elegans is one of the major model systems in biology based on advantageous properties such as short life span, transparency, genetic tractability and ease of culture using an escherichia coli diet. in its natural habitat, compost and rotting plant material, this nematode lives on bacteria. however, c. elegans is a predator of bacteria, but can also be infected by nematopathogenic coryneform bacteria such microbacterium and leucobacter species, which display intriguing and diverse ...	2016	26490043
branched-chain amino acids.	branched-chain amino acids (bcaas), viz., l-isoleucine, l-leucine, and l-valine, are essential amino acids that cannot be synthesized in higher organisms and are important nutrition for humans as well as livestock. they are also valued as synthetic intermediates for pharmaceuticals. therefore, the demand for bcaas in the feed and pharmaceutical industries is increasing continuously. traditional industrial fermentative production of bcaas was performed using microorganisms isolated by random muta ...	2016	27872960
biotechnological production of aromatic compounds of the extended shikimate pathway from renewable biomass.	aromatic chemicals that contain an unsaturated ring with alternating double and single bonds find numerous applications in a wide range of industries, e.g. paper and dye manufacture, as fuel additives, electrical insulation, resins, pharmaceuticals, agrochemicals, in food, feed and cosmetics. their chemical production is based on petroleum (btx; benzene, toluene, and xylene), but they can also be obtained from plants by extraction. due to petroleum depletion, health compliance, or environmental ...	2016	27871872
exporters for production of amino acids and other small molecules.	microbes are talented catalysts to synthesize valuable small molecules in their cytosol. however, to make full use of their skills - and that of metabolic engineers - the export of intracellularly synthesized molecules to the culture medium has to be considered. this step is as essential as is each step for the synthesis of the favorite molecule of the metabolic engineer, but is frequently not taken into account. to export small molecules via the microbial cell envelope, a range of different typ ...	2016	27832297
lysine fermentation: history and genome breeding.	lysine fermentation by corynebacterium glutamicum was developed in 1958 by kyowa hakko kogyo co. ltd. (current kyowa hakko bio co. ltd.) and is the second oldest amino acid fermentation process after glutamate fermentation. the fundamental mechanism of lysine production, discovered in the early stages of the process's history, gave birth to the concept known as "metabolic regulatory fermentation," which is now widely applied to metabolite production. after the development of rational metabolic e ...	2016	27832296
increased production of food-grade d-tagatose from d-galactose by permeabilized and immobilized cells of corynebacterium glutamicum, a gras host, expressing d-galactose isomerase from geobacillus thermodenitrificans.	the generally recognized as safe microorganism corynebacterium glutamicum expressing geobacillus thermodenitrificans d-galactose isomerase (d-gai) was an efficient host for the production of d-tagatose, a functional sweetener. the d-tagatose production at 500 g/l d-galactose by the host was 1.4-fold higher than that by escherichia coli expressing d-gai. the d-tagatose-producing activity of permeabilized c. glutamicum (pcg) cells treated with 1% (w/v) triton x-100 was 2.1-fold higher than that of ...	2016	27734668
development of a genetically engineered escherichia coli strain for plasmid transformation in corynebacterium glutamicum.	gene disruption and replacement in corynebacterium glutamicum is dependent upon a high transformation efficiency. the cglir-cgiir restriction system is a major barrier to introduction of foreign dna into corynebacterium glutamicum cells. to improve the transformation efficiency of c. glutamicum, the cglim gene encoding methyltransferase in the cglir-cgliir-cglim restriction-modification system of c. glutamicum atcc 13032 was chromosomally integrated and expressed in escherichia coli, resulting i ...	2016	27793586
a new metabolic route for the production of gamma-aminobutyric acid by corynebacterium glutamicum from glucose.	gamma-aminobutyric acid (gaba), a non-protein amino acid widespread in nature, is a component of pharmaceuticals, foods, and the biodegradable plastic polyamide 4. corynebacterium glutamicum shows great potential for the production of gaba from glucose. gaba added to the growth medium hardly affected growth of c. glutamicum, since a half-inhibitory concentration of 1.1 m gaba was determined. as alternative to gaba production by glutamate decarboxylation, a new route for the production of gaba vi ...	2016	27289384
overexpression of methionine adenosyltransferase in corynebacterium glutamicum for production of s-adenosyl-l-methionine.	two genes encoding methionine adenosyltransferase, sam2 from saccharomyces cerevisiae and metk from corynebacterium glutamicum, were individually cloned into pdxw-8, the shuttle vector between escherichia coli and c. glutamicum, and overexpressed in e. coli dh5α and c. glutamicum atcc13032. in dh5α, both genes were overexpressed and their protein products showed the activity of methionine adenosyltransferase. in atcc13032, metk was overexpressed, its product metk showed the enzyme activity and c ...	2016	26238196
stereospecificity of corynebacterium glutamicum 2,3-butanediol dehydrogenase and implications for the stereochemical purity of bioproduced 2,3-butanediol.	the stereochemistry of 2,3-butanediol (2,3-bd) synthesis in microbial fermentations is important for many applications. in this work, we showed that corynebacterium glutamicum endowed with the lactococcus lactis genes encoding α-acetolactate synthase and decarboxylase activities produced meso-2,3-bd as the major end product, meaning that (r)-acetoin is a substrate for endogenous 2,3-butanediol dehydrogenase (bdh) activity. this is curious in view of the reported absolute stereospecificity of c. ...	2016	27687994
the topology of the l-arginine exporter argo conforms to an nin-cout configuration in escherichia coli: requirement for the cytoplasmic n-terminal domain, functional helical interactions, and an aspartate pair for argo function.	argo and lyse are members of the lyse family of exporter proteins and ordinarily mediate the export of l-arginine (arg) in escherichia coli and l-lysine (lys) and arg in corynebacterium glutamicum, respectively. under certain conditions, argo also mediates lys export. to delineate the arrangement of argo in the cytoplasmic membrane of e. coli, we have employed a combination of cysteine accessibility in situ, alkaline phosphatase fusion reporters, and protein modeling to arrive at a topological m ...	2016	27645388
time-resolved, single-cell analysis of induced and programmed cell death via non-invasive propidium iodide and counterstain perfusion.	conventional propidium iodide (pi) staining requires the execution of multiple steps prior to analysis, potentially affecting assay results as well as cell vitality. in this study, this multistep analysis method has been transformed into a single-step, non-toxic, real-time method via live-cell imaging during perfusion with 0.1 μm pi inside a microfluidic cultivation device. dynamic pi staining was an effective live/dead analytical tool and demonstrated consistent results for single-cell death in ...	2016	27580964
high-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in escherichia coli.	succinate has been identified by the u.s. department of energy as one of the top 12 building block chemicals, which can be used as a specialty chemical in the agricultural, food, and pharmaceutical industries. escherichia coli are now one of the most important succinate producing candidates. however, the stoichiometric maximum succinate yield under anaerobic conditions through the reductive branch of the tca cycle is restricted by nadh supply in e. coli.	2016	27520031
the flexible feedstock concept in industrial biotechnology: metabolic engineering of escherichia coli, corynebacterium glutamicum, pseudomonas, bacillus and yeast strains for access to alternative carbon sources.	most biotechnological processes are based on glucose that is either present in molasses or generated from starch by enzymatic hydrolysis. at the very high, million-ton scale production volumes, for instance for fermentative production of the biofuel ethanol or of commodity chemicals such as organic acids and amino acids, competing uses of carbon sources e.g. in human and animal nutrition have to be taken into account. thus, the biotechnological production hosts e. coli, c. glutamicum, pseudomona ...	2016	27491712
d-allulose production from d-fructose by permeabilized recombinant cells of corynebacterium glutamicum cells expressing d-allulose 3-epimerase flavonifractor plautii.	a d-allulose 3-epimerase from flavonifractor plautii was cloned and expressed in escherichia coli and corynebacterium glutamicum. the maximum activity of the enzyme purified from recombinant e. coli cells was observed at ph 7.0, 65°c, and 1 mm co2+ with a half-life of 40 min at 65°c, km of 162 mm, and kcat of 25280 1/s. for increased d-allulose production, recombinant c. glutamicum cells were permeabilized via combined treatments with 20 mg/l penicillin and 10% (v/v) toluene. under optimized con ...	2016	27467527
progress in the microbial production of s-adenosyl-l-methionine.	s-adenosyl-l-methionine (sam), which exists in all living organisms, serves as an activated group donor in a range of metabolic reactions, including trans-methylation, trans-sulfuration and trans-propylamine. compared with its chemical synthesis and enzyme catalysis production, the microbial production of sam is feasible for industrial applications. the current clinical demand for sam is constantly increasing. therefore, vast interest exists in engineering the sam metabolism in cells for increas ...	2016	27465853
engineering a glycerol utilization pathway in corynebacterium glutamicum for succinate production under o2 deprivation.	to explore the glycerol utilization pathway in corynebacterium glutamicum for succinate production under o2 deprivation.	2016	27395064
enhanced succinate production from glycerol by engineered escherichia coli strains.	in this study, an engineered strain escherichia coli mlb (ldha(-)pflb(-)) was constructed for production of succinate from glycerol. the succinate yield was 0.37mol/mol in anaerobic culture, however, the growth and glycerol consumption rates were very slow, resulting in a low succinate level. two-stage fermentation was performed in flasks, and the succinate yield reached 0.93mol/mol, but the succinate titer was still low. hence, overexpression of malate dehydrogenase, malic enzyme, phosphoenolpy ...	2016	27371794
roles of export genes cgma and lyse for the production of l-arginine and l-citrulline by corynebacterium glutamicum.	l-arginine is a semi-essential amino acid with application in cosmetic, pharmaceutical, and food industries. metabolic engineering strategies have been applied for overproduction of l-arginine by corynebacterium glutamicum. lyse was the only known l-arginine exporter of this bacterium. however, an l-arginine-producing strain carrying a deletion of lyse still accumulated about 10 mm l-arginine in the growth medium. overexpression of the putative putrescine and cadaverine export permease gene cgma ...	2016	27350619
three-steps in one-pot: whole-cell biocatalytic synthesis of enantiopure (+)- and (-)-pinoresinol via kinetic resolution.	pinoresinol is a high-value plant-derived lignan with multiple health supporting effects. enantiomerically pure pinoresinol can be isolated from natural sources, but with low efficiency. most chemical and biocatalytic approaches that have been described for the synthesis of pinoresinol furnish the racemic mixture. in this study we devised a three-step biocatalytic cascade for the production of enantiomerically pure pinoresinol from the cheap compound eugenol. two consecutive oxidations of eugeno ...	2016	27160378
updates on industrial production of amino acids using corynebacterium glutamicum.	l-amino acids find various applications in biotechnology. l-glutamic acid and its salts are used as flavor enhancers. other l-amino acids are used as food or feed additives, in parenteral nutrition or as building blocks for the chemical and pharmaceutical industries. l-amino acids are synthesized from precursors of central carbon metabolism. based on the knowledge of the biochemical pathways microbial fermentation processes of food, feed and pharma amino acids have been developed. production str ...	2016	27116971
properties of cassava starch modified by amylomaltase from corynebacterium glutamicum.	amylomaltase (α-1,4-glucanotransferase, am; ec 2.4.1.25) from corynebacterium glutamicum expressed in escherichia coli was used to prepare the enzyme-modified cassava starch for food application. about 5% to 15% (w/v) of cassava starch slurries were incubated with 1, 3, or 5 units of amylomaltase/g starch. apparent amylose, amylopectin chain length distribution, thermal properties, freeze-thaw stability, thermo-reversibility, and gel strength of the obtained modified starches were measured. the ...	2016	27105125
influence of nanomechanical stress induced by zno nanoparticles of different shapes on the viability of cells.	there is growing interest in nanostructures interacting with living organisms. however, there are still no general rules for the design of biocompatible nanodevices. here, we present a step towards understanding the interactions between nanostructures and living cells. we study the influence of nanomechanical stress induced by zinc oxide (zno) nanostructures of different shapes on the viability of both prokaryotic (gram-negative bacteria: escherichia coli and enterobacter aerogenes, and gram-pos ...	2016	27074722
dihydroxyacetone production in an engineered escherichia coli through expression of corynebacterium glutamicum dihydroxyacetone phosphate dephosphorylase.	dihydroxyacetone (dha) has several industrial applications such as a tanning agent in tanning lotions in the cosmetic industry; its production via microbial fermentation would present a more sustainable option for the future. here we genetically engineered escherichia coli (e. coli) for dha production from glucose. deletion of e. coli triose phosphate isomerase (tpia) gene was carried out to accumulate dihydroxyacetone phosphate (dhap), for use as the main intermediate or precursor for dha produ ...	2016	26992791
production of succinic acid by metabolically engineered microorganisms.	succinic acid (sa) has been recognized as one of the most important bio-based building block chemicals due to its numerous potential applications. for the economical bio-based production of sa, extensive research works have been performed on developing microbial strains by metabolic engineering as well as fermentation and downstream processes. here we review metabolic engineering strategies applied for bio-based production of sa using representative microorganisms, including saccharomyces cerevi ...	2016	26990278
pathway construction and metabolic engineering for fermentative production of ectoine in escherichia coli.	ectoine is a protective agent and stabilizer whose synthesis pathway exclusively exists in select moderate halophiles. a novel established process called "bacterial milking" efficiently synthesized ectoine in moderate halophiles, however, this method places high demands on equipment and is cost prohibitive. in this study, we constructed an ectoine producing strain by introducing the ectoine synthesis pathway into escherichia coli and improved its production capacity. firstly, the ectabc gene clu ...	2016	26969253
a new strategy for production of 5-aminolevulinic acid in recombinant corynebacterium glutamicum with high yield.	5-aminolevulinic acid (ala), a nonprotein amino acid involved in tetrapyrrole synthesis, has been widely applied in agriculture, medicine, and food production. many engineered metabolic pathways have been constructed; however, the production yields are still low. in this study, several 5-aminolevulinic acid synthases (alass) from different sources were evaluated and compared with respect to their ala production capacities in an engineered corynebacterium glutamicum cgs1 strain that can accumulat ...	2016	26921424
microfluidic screening of electric fields for electroporation.	electroporation is commonly used to deliver molecules such as drugs, proteins, and/or dna into cells, but the mechanism remains poorly understood. in this work a rapid microfluidic assay was developed to determine the critical electric field threshold required for inducing bacterial electroporation. the microfluidic device was designed to have a bilaterally converging channel to amplify the electric field to magnitudes sufficient to induce electroporation. the bacterial cells are introduced into ...	2016	26893024
strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression.	with the availability of the whole genome sequence of escherichia coli or corynebacterium glutamicum, strategies for directed dna manipulation have developed rapidly. dna manipulation plays an important role in understanding the function of genes and in constructing novel engineering bacteria according to requirement. dna manipulation involves modifying the autologous genes and expressing the heterogenous genes. two alternative approaches, using electroporation linear dna or recombinant suicide ...	2016	26834010
web application for genetic modification flux with database to estimate metabolic fluxes of genetic mutants.	computational analysis of metabolic fluxes is essential in understanding the structure and function of a metabolic network and in rationally designing genetically modified mutants for an engineering purpose. we had presented the genetic modification flux (gmf) that predicts the flux distribution of a broad range of genetically modified mutants. to enhance the feasibility and usability of gmf, we have developed a web application with a metabolic network database to predict a flux distribution of ...	2016	26777238
in vitro functional characterization of the na+/h+ antiporters in corynebacterium glutamicum.	corynebacterium glutamicum, typically used as industrial workhorse for amino acid production, is a moderately salt-alkali-tolerant microorganism with optimal growth at ph 7-9. however, little is known about the mechanisms of salt-alkali tolerance in c. glutamicum. here, the catalytic capacity of three putative na(+)/h(+) antiporters from c. glutamicum (designated as cg-mrp1, cg-mrp2 and cg-nhap) were characterized in an antiporter-deficient escherichia coli knabc strain. only cg-mrp1 was able to ...	2016	26667218
enhanced production of 3-hydroxypropionic acid from glucose via malonyl-coa pathway by engineered escherichia coli.	in this study, production of 3-hp via malonyl-coa was investigated by using metabolically engineered escherichia coli carrying heterogeneous acetyl-coa carboxylase (acc) from corynebacterium glutamicum and codon-optimized malonyl-coa reductase (mcr) from chloroflexus aurantiacus. three engineered e. coli strains with different host-vector systems were constructed and investigated. the results indicated that the combination of e. coli bl21(de3) and pet28a was the most efficient host-vector system ...	2016	26606325
fudc, a protein primarily responsible for furfural detoxification in corynebacterium glutamicum.	lignocellulosic hydrolysates contain compounds that inhibit microbial growth and fermentation, thereby decreasing the productivity of biofuel and biochemical production. in particular, the heterocyclic aldehyde furfural is one of the most toxic compounds found in these hydrolysates. we previously demonstrated that corynebacterium glutamicum converts furfural into the less toxic compounds furfuryl alcohol and 2-furoic acid. to date, however, the genes involved in these oxidation and reduction rea ...	2016	26541332
the impact of the c-terminal domain on the gating properties of msccg from corynebacterium glutamicum.	the mechanosensitive (ms) channel msccg from the soil bacterium corynebacterium glutamicum functions as a major glutamate exporter. msccg belongs to a subfamily of the bacterial mscs-like channels, which play an important role in osmoregulation. to understand the structural and functional features of msccg, we investigated the role of the carboxyl-terminal domain, whose relevance for the channel gating has been unknown. the chimeric channel mscs-(c-msccg), which is a fusion protein between the c ...	2016	26494188
production of protocatechuic acid by corynebacterium glutamicum expressing chorismate-pyruvate lyase from escherichia coli.	protocatechuic acid (3,4-dihydroxybenzoic acid; pca) serves as a building block for polymers and pharmaceuticals. in this study, the biosynthetic pathway for pca from glucose was engineered in corynebacterium glutamicum. the pathway to pca-employed elements of the chorismate pathway by using chorismate-pyruvate lyase (cpl) and 4-hydroxybenzoate hydroxylase (4-hba hydroxylase). as c. glutamicum has the potential to synthesize the aromatic amino acid intermediate chorismate and possesses 4-hba hyd ...	2016	26392137
implication of ornithine acetyltransferase activity on l-ornithine production in corynebacterium glutamicum.	l-ornithine is an intermediate of the l-arginine biosynthetic pathway in corynebacterium glutamicum. the effect of ornithine acetyltransferase (oatase; argj) on l-ornithine production was investigated, and c. glutamicum 1006 was engineered to overproduce l-ornithine as a major product by inactivating regulatory repressor argr gene and overexpressing argj gene. a genome sequence analysis indicated that the argf gene encoding ornithine carbamoyltransferase in c. glutamicum 1006 was mutated, result ...	2016	25630515
metabolic engineering of corynebacterium glutamicum for the de novo production of ethylene glycol from glucose.	development of sustainable biological process for the production of bulk chemicals from renewable feedstock is an important goal of white biotechnology. ethylene glycol (eg) is a large-volume commodity chemical with an annual production of over 20 million tons, and it is currently produced exclusively by petrochemical route. herein, we report a novel biosynthetic route to produce eg from glucose by the extension of serine synthesis pathway of corynebacterium glutamicum. the eg synthesis is achie ...	2016	26556130
corynebacterium glutamicum ggtb encodes a functional γ-glutamyl transpeptidase with γ-glutamyl dipeptide synthetic and hydrolytic activity.	in this work the role of γ-glutamyl transpeptidase in the metabolism of γ-glutamyl dipeptides produced by corynebacterium glutamicum atcc 13032 was studied. the enzyme is encoded by the gene ggtb (cg1090) and synthesized as a 657 amino acids long preprotein. gamma-glutamyl transpeptidase activity was found to be associated with intact cells of c. glutamicum and was abolished upon deletion of ggtb. bioinformatic analysis indicated that the enzyme is a lipoprotein and is attached to the outer side ...	2016	26528625
application of crispri in corynebacterium glutamicum for shikimic acid production.	to construct, test and exploit the crispri system for enhancement of shikimic acid production with corynebacterium glutamicum.	2016	27623797
construction of genetic parts from the corynebacterium glutamicum genome with high expression activities.	to construct effective genetic expression parts controlling transcription and translation initiation for synthetic biology and heterologous expression in corynebacterium glutamicum.	2016	27580890
rnase iii mediated cleavage of the coding region of mraz mrna is required for efficient cell division in corynebacterium glutamicum.	the corynebacterium glutamicum r cgr_1959 gene encodes an endoribonuclease of the rnase iii family. deletion mutant of cgr_1959 (δrnc mutant) showed an elongated cell shape, and presence of several lines on the cell surface, indicating a required of rnase iii for maintaining normal cell morphology in c. glutamicum. the level of mraz mrna was increased, whereas cgr_1596 mrna encoding a putative cell wall hydrolase and ftsex mrna were decreased in the δrnc mutant. the half-life of mraz mrna was si ...	2016	26713407
high-titer biosynthesis of hyaluronic acid by recombinant corynebacterium glutamicum.	hyaluronic acid (ha) plays important roles in human tissue system, thus it is highly desirable for various applications, such as in medical, clinic and cosmetic fields. the wild microbial producer of ha, streptococcus, was restricted by its potential pathogens, hence different recombinant hosts are being explored. in this work, we engineered corynebacterium glutamicum, a gras (generally recognized as safe) organism free of exotoxins and endotoxins to produce ha with high titer and satisfied mw . ...	2016	26709615
altered acetylation and succinylation profiles in corynebacterium glutamicum in response to conditions inducing glutamate overproduction.	the bacterium corynebacterium glutamicum is utilized during industrial fermentation to produce amino acids such as l-glutamate. during l-glutamate fermentation, c. glutamicum changes the flux of central carbon metabolism to favor l-glutamate production, but the molecular mechanisms that explain these flux changes remain largely unknown. here, we found that the profiles of two major lysine acyl modifications were significantly altered upon glutamate overproduction in c. glutamicum; acetylation de ...	2016	26663479
metabolic engineering corynebacterium glutamicum to produce triacylglycerols.	in this study, we metabolically engineered corynebacterium glutamicum to produce triacylglycerols (tags) by completing and constraining a de novo tag biosynthesis pathway. first, the plasmid pz8_tag4 was constructed which allows the heterologous expression of four genes: three (atf1 and atf2, encoding the diacylglycerol acyltransferase; pgpb, encoding the phosphatidic acid phosphatase) to complete the tag biosynthesis pathway, and one gene (tada) for lipid body assembly. second, we applied four ...	2016	26645801
metabolic engineering of corynebacterium glutamicum for efficient production of 5-aminolevulinic acid.	5-aminolevulinic acid (5-ala) has recently attracted attention for its potential applications in the fields of medicine and agriculture. in this study, corynebacterium glutamicum was firstly engineered for 5-ala production via the c4 pathway. hema encoding 5-aminolevulinic acid synthase from rhodobacter sphaeroides was codon optimized and expressed in c. glutamicum atcc13032, resulting in accumulation of 5-ala. deletion of all known genes responsible for the formation of acetate and lactate furt ...	2016	26616115
identification of the phd gene cluster responsible for phenylpropanoid utilization in corynebacterium glutamicum.	phenylpropanoids as abundant, lignin-derived compounds represent sustainable feedstocks for biotechnological production processes. we found that the biotechnologically important soil bacterium corynebacterium glutamicum is able to grow on phenylpropanoids such as p-coumaric acid, ferulic acid, caffeic acid, and 3-(4-hydroxyphenyl)propionic acid as sole carbon and energy sources. global gene expression analyses identified a gene cluster (cg0340-cg0341 and cg0344-cg0347), which showed increased tr ...	2016	26610800
formation of xylitol and xylitol-5-phosphate and its impact on growth of d-xylose-utilizing corynebacterium glutamicum strains.	wild-type corynebacterium glutamicum has no endogenous metabolic activity for utilizing the lignocellulosic pentose d-xylose for cell growth. therefore, two different engineering approaches have been pursued resulting in platform strains harbouring a functional version of either the isomerase (iso) or the weimberg (wmb) pathway for d-xylose assimilation. in a previous study we found for c. glutamicum wmb by-product formation of xylitol during growth on d-xylose and speculated that the observed l ...	2016	27297548
corynebacterium glutamicum mtcc 2745 immobilized on granular activated carbon/mnfe2o4 composite: a novel biosorbent for removal of as(iii) and as(v) ions.	the optimization of biosorption/bioaccumulation process of both as(iii) and as(v) has been investigated by using the biosorbent; biofilm of corynebacterium glutamicum mtcc 2745 supported on granular activated carbon/mnfe2o4 composite (mgac). the presence of functional groups on the cell wall surface of the biomass that may interact with the metal ions was proved by ft-ir. to determine the most appropriate correlation for the equilibrium curves employing the procedure of the non-linear regression ...	2016	27289352
transcription of sialic acid catabolism genes in corynebacterium glutamicum is subject to catabolite repression and control by the transcriptional repressor nanr.	corynebacterium glutamicum metabolizes sialic acid (neu5ac) to fructose-6-phosphate (fructose-6p) via the consecutive activity of the sialic acid importer siaefgi, n-acetylneuraminic acid lyase (nana), n-acetylmannosamine kinase (nank), n-acetylmannosamine-6p epimerase (nane), n-acetylglucosamine-6p deacetylase (naga), and glucosamine-6p deaminase (nagb). within the cluster of the three operons nagab, nanake, and siaefgi for neu5ac utilization a fourth operon is present, which comprises cg2936, ...	2016	27274030
use of in vitro transcription system for analysis of corynebacterium glutamicum promoters recognized by two sigma factors.	promoter activities in corynebacterium glutamicum strains with deletions of genes encoding sigma factors of rna polymerase suggested that transcription from some promoters is controlled by two sigma factors. to prove that different sigma factors are involved in the recognition of selected corynebacterium glutamicum promoters, in vitro transcription system was applied. it was found that a typical housekeeping promoter pper interacts with the alternative sigma factor σ(b) in addition to the primar ...	2016	27270733
metabolic engineering of corynebacterium glutamicum for methionine production by removing feedback inhibition and increasing nadph level.	relieving the feedback inhibition of key enzymes in a metabolic pathway is frequently the first step of producer-strain construction by genetic engineering. however, the strict feedback regulation exercised by microorganisms in methionine biosynthesis often makes it difficult to produce methionine at a high level. in this study, corynebacterium glutamicum atcc 13032 was metabolically engineered for methionine production. first, the metd gene encoding the methionine uptake system was deleted to a ...	2016	27255137