Publications

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[the microbiological test as a method for determination of plant growth regulators]. 19684968774
inhibition of "serine" esterases by phenylarsonic acids. alpha-chymotrypsin and the subtilisins. 19684968801
influence of spore moisture content on the dry-heat resistance of bacillus subtilis var. niger.the dry-heat resistance of bacillus subtilis var. niger spores located in or on various materials was determined as d and z values in the range of 105 through 160 c. the systems tested included spores located on steel and paper strips, spores located between stainless-steel washers mated together under 150 inch-lb and 12 inch-lb of torque, and spores encapsulated in methylmethacrylate and epoxy plastics. d values for a given temperature varied with the test system. high d values were observed fo ...19684968962
metabolism of tryptophans by pseudomonas aureofaciens. vi. production of pyrrolnitrin by selected pseudomonas species.twenty-nine strains of pseudomonas, classified as p. fluorescens biotype d or e or as p. multivorans, were examined for the production of pyrrolnitrin, an antifungal agent synthesized in p. aureofaciens. eight strains were shown to produce pyrrolnitrin in shake-flask fermentation. four cultures were from the multivorans taxon, and the remaining four were members of the fluorescens group. the antifungal agent produced in these strains was isolated and shown to be pyrrolnitrin by comparison with a ...19684968963
sporicidal activity of sodium hypochlorite at subzero temperatures.sodium hypochlorite was an excellent disinfectant at low temperatures. with the addition of ethylene glycol to prevent freezing, hypochlorite solutions at low free available chlorine concentrations, were effective against bacillus subtilis var. niger spores from 0 to -40 c. the effectiveness of this decontaminant was influenced by temperature, ph, and concentration, with ph 7.2 the optimum for decontamination at all temperatures and concentrations.19684968965
selective inhibition of initiation of germination of bacterial endospores. 19684968967
influence of gamma-irradiation and heating in the presence of a bactericide on the inactivation of bacillus subtilis spores. 19684968968
effect of bacillus subtilis on growth and sclerotium formation by rhizoctonia solani. 19684969085
[bacillus subtilis in therapy and prevention of disease]. 19684969138
inactivation and mutagenesis on isolated dna. ii. kinetics of mutagenesis and efficiency of different mutagens. 19684969219
phase transitions in ribonucleic acid synthesis during germination of bacillus subtilis spores. 19684969222
[action of urea on the anzymatic activity of levan-sucrase of b. subtilis]. 19684969236
transformation in bacillus cereus 569: a correction of strain designation. 19684969794
a reversible inhibition of the germination of bacterial spores. 19684969803
organization of polymers in the cell walls of some bacilli. 19684969845
characteristics of extracellular protease formation by bacillus subtilis and its control by amino acid repression. 19684969865
the use of biochemical mutants of test-bacteria in the screening programs with streptomyces. 19684969598
deoxyribonucleic acid antibody: a method to detect its primary interaction with deoxyribonucleic acid.antibody to dna in human serums can be detected by the ammonium sulfate method. this sensitive and specific technique, which measures the primary interaction between dna and antibody to dna, is based on the observation that free dna is soluble in 50-percent saturated ammonium sulfate whereas antibody-bound dna is insoluble.19684969617
kinetics of death induced by penicillin and chloramphenicol in synchronous cultures of escherichia coli. 19684969433
[decisive importance of the correlation of nuclei, mitochondria and the culture medium containing kanamycin in the study of its interaction with the cellular components and intracellular]. 19684969471
properties of escherichia coli deoxyribonucleoprotein as compared with the deoxyribonucleoprotein of higher organisms. 19684968582
synthesis of teichoic acids. vii. synthesis of teichoic acids during spore germination.the synthesis of teichoic acids has been examined during germination in bacillus licheniformis atcc 9945 and in b. subtilis w-23. teichoic acids are absent from the spores of both organisms. b. licheniformis spores lack the enzymes responsible for teichoic acid synthesis. the appearance of these enzymes during germination is correlated with the appearance of teichoic acids in the cell. the appearance of teichoic acid-synthesizing enzymes and of teichoic acids in the cell are inhibited by the add ...19684970221
new procedures for purification of l-asparaginase with high yield from escherichia coli.l-asparaginase is now known to be a potent antineoplastic agent in animals and has given complete remission in some human leukemias. extensive clinical trials of this enzyme, however, were not possible in the past because of inadequate production of this substance. we have developed practical procedures for producing l-asparaginase in yields of sufficient quantity and purity for more extensive clinical evaluation. the nutritional requirements for optimal production of biologically active l-aspar ...19684970225
chromosomes in bacillus subtilis spores and their segregation during germination.spores of a thymine-requiring mutant of bacillus subtilis 168 leucine(-), indole(-), thymine(-)) were uniformly labeled with (3)h-thymidine. these were seeded on thinlayer agar plates where they germinated into long-chained microcolonies. autoradiograms were used to measure the distribution of labeled deoxyribonucleic acid in the chains of cells, which ranged in length from 2 to 32 cells. four major grain clusters appeared in most chains. these clusters were homogeneous in size; their grain numb ...19684970228
genetic mapping of a locus which regulates the production of pigment associated with spores of bacillus subtilis. 19684970229
enhancement and inhibition of transformation in bacillus subtilis. 19684970230
pathway of purine nucleotide synthesis in bacillus subtilis. 19684970231
inhibition of the synthesis of the alkaline phosphatase of bacillus megarterium km by a novel protoplast-bursting factor obtained from bacillus subtilis 202-7. 19684969953
effects of high electric fields on micro-organisms. 3. lysis of erythrocytes and protoplasts. 19684969954
[some investigations of sterols in bacteria]. 19684969995
adsorption of vancomycin by sensitive and resistant organisms. 19684972066
regulation of synthesis of 2,3-dihydroxybenzoic acid in bacillus subtilis by iron and a biological secondary hydroxamate. 19684972067
the isolation and mode of action of a bacterial glucanosyltransferase. 19684972097
mutations and decreases in density of transforming dna produced by derivatives of the carcinogens 2-acetyl-aminofluorene and n-methyl-4-aminoazobenzene. 19684972127
on the inhibition of ribonucleic synthesis in bacillus subtilis cells by steffimycin. 19684972144
protein chain initiation and deformylation in b. subtilis homogenates. 19684970729
[study of bruneomycin formed by act. albus var. bruneomycini and its identification with streptonigrin]. 19684970063
lytic factor and competence in bacillus subtilis. 19684971328
streptomycin-like compound enzymically released from a streptomycin-non-producing streptomyces griseus strain. 19684971329
transformation of lysozyme spheroplasts of bacillus subtilis. 19684971337
effect of pactamycin on the synthesis of rna and protein and on methylation of rna. 19684971340
biochemical studies of bacterial sporulation and germination. ix. ribonucleic acid and deoxyribonucleic acid polymerases in nuclear fractions of vegetative cells and spores of bacillus megaterium. 19684971347
induction and repression of the histidine-degrading enzymes of bacillus subtilis. 19684971350
[derivatives of dipheoro-acetic acid with antibacterial activity. 3]. 19684971381
effect of ultraviolet irradiation on the mineral uptake and osmotic barrier of certain bacteria. 19684971383
chemical analysis of dna alterations. 3. isolation and characterization of adenine oxidation products obtained from oligo- and monodeoxyadenylic acids treated with hydroxyl radicals. 19684971400
selective inhibition of extracellular enzyme synthesis by removal of cell wall from bacillus subtilis. 19684971402
ultraviolet resistance of dna in spore spheroplasts of bacillus subtilis as measured by the transforming activity. 19684971403
the effect of antibiotics on the results of the guthrie test given to phenylketonuric patients. 19684971439
rhodotorulic acid, a diketopiperazine dihydroxamic acid with growth-factor activity. i. isolation and characterization. 19684971459
[study on the occurrence of secondary bacterial infections in swine affected with hog cholera with special reference to enterobacteriaceae]. 19684971489
a new antibacterial agent produced by streptomyces sp. ac6-569. 19684971618
[on the washing of hands in a pediatric ward. on the advantage of combining an organic mercurial disinfectant with a hexachlorophene]. 19684971633
effects of bacillus subtilis protease, type 8 (subtilopeptidase a) on isolated adipose cells. fructose utilization and its inhibition by avenaciolide, piericidin a and puromycin. 19684971641
phenolic acids and iron transport in bacillus subtilis. 19684971642
mutagenesis by acridine yellow in bacillus subtilis. 19684971657
biochemical studies of bacterial sporulation and germination. 8. patterns of enzyme development during growth and sporulation of baccillus subtilis. 19684971658
biochemical studies of bacterial sporulation and germination. vi. origin of spore core and coat proteins. 19684971698
biochemical studies of bacterial sporulation and germaination. vii. protein turnover during sporulation of bacillus subtilis. 19684971699
evaluation of laminar flow microbiological safety cabinets.the microbiological control efficiency of two class 100 laminar down-flow hoods was determined by using aerosols of bacillus subtilis var. niger spores. the first unit challenged utilized a slanted eyelid to partially enclose the front work opening. this hood showed nearly perfect control of ambient organisms in the work area. it also gave a 10(6) or greater drop in the number of organisms passing out of the exhaust system. however, when the interior work area of the hood was challenged, signifi ...19684971719
a new assay for phage hydroxymethylases and its use in bacillus subtilis transfection. 19684971740
use of b. subtilis neutral proteinase in blood group serology. 19684971759
the conformation of the 50s ribosomal subunit of bacillus subtilis. 19684971841
curing of a sporulation mutant and antibiotic activity of bacillus subtilis.sporulation mutants of bacillus subtilis, blocked either in the citric acid cycle or in another pathway necessary for uracil incorporation during the developmental period, were tested for their sporulation response to different carbon sources. all of the citric acid cycle mutants and all but one of the other mutants failed to respond. the one phenotypically curable mutant, 60764, responded to most metabolizable carboxylic acids, such as acetate and fatty acids, and to some other carbon sources. ...19684971886
characterization of mutants with single and multiple defects in the tryptophan biosynthetic pathway in bacillus subtilis.sixty-five tryptophan auxotrophs which map in a cluster on the genome of bacillus subtilis were characterized on the basis of (i) growth response, (ii) accumulation of intermediate compounds, and (iii) determination of enzymatic defects. they could be placed into six phenotypic classes. certain of the mutants exhibited pleiotropic effects on more than one enzymatic activity in a manner different from those effects reported for the tryptophan pathway in other organisms. invariably, mutations in t ...19684971887
parallel between ultraviolet light and 4-nitroquinoline-1-oxide sensitivity in bacillus subtilis. 19684971891
active sites in the wash. 19684971956
studies on the in vitro synthesis of transforming dna. 19684972012
correlation of the sulfhydryl group with the essential calcium in bacillus subtilis saccharifying alpha-amylase. 19684970390
new basic water-soluble antibiotics bd-12 and by-81. i. taxonomy of the producing organisms and antibiotic production. 19684970417
immunofluorescent f-p ratios of some b. subtilis conjugates for use with microporous filters. 19684970437
proteolytic activity on an insoluble protein piled up by an asporogenic mutant of bacillus subtilis. 19684970443
a spectrophotometric assay for neutral protease. 19684970444
the action of the bacillus subtilis neutral protease on s-beta-aminoethylcysteinyl ferredoxin from clostridium pasteurianum. 19684970480
the formation of a vasopressor polypeptide by subtilisin carlsberg (subtilisin), a proteolytic enzyme of bacillus subtilis. 19684970519
effect of azalomycin f on bacteria. 19684970571
studies on the action of o-carbamyl-d-serine in bacillus subtilis. 19684970599
single strand interruptions in pbs 1 bacteriophage dna molecule. 19684970601
a comparison of the segregation of chromosomes within microcolonies developing from single bacillus subtilis and bacillus megaterium spores. 19684970602
ribosome formation and structure. 19684970633
ratio of teichoic acid and peptidoglycan in cell walls of bacillus subtilis following spire germination and during vegetative growth.cell walls were isolated from cells of bacillus subtilis strain marburg during synchronous outgrowth of spores, during the two synchronous cell divisions which followed, and at various times during exponential and early stationary growth. the amounts of teichoic acid and peptidoglycan components were determined in each cell wall preparation. the peptidoglycan is composed of hexosamine, alanine, diaminopimelic acid, and glutamic acid. the ratio of these was relatively constant in the cell walls a ...19684970648
characteristics of secretion of penicillinase, alkaline phosphatase, and nuclease by bacillus species.the distribution of alkaline phosphatase and nuclease activity between cells and medium was examined in one strain of bacillus licheniformis and four strains of b. subtilis. over 95% of both activities was found in the medium of the b. licheniformis culture, but in the b. subtilis cultures the amount of enzyme activity found in the medium varied with the strain and the enzyme considered. b. licheniformis 749 and its penicillinase magnoconstitutive mutant 749/c were grown in continuous culture wi ...19684970649
properties of 5-fluorouracil-containing ribonucleic acid and ribosomes from bacillus subtilis.growth of a strain of bacillus subtilis that requires uracil, thymine, adenine, and tryptophan in the presence of 5-fluorouracil (fu) results in the synthesis of ribonucleic acid (rna) and ribosomes in which 55 to 65% of the rna uracil has been replaced by the fluorine derivative. examination of analogue-containing ribosomes by sucrose density gradient centrifugation and thermal denaturation studies suggests that, as far as the size, shape, and packing structure are concerned, extensive fu subst ...19684970652
effect of 5-fluorouracil on the growth and morphology of a polyauxotrophic strain of bacillus subtilis. 19684970653
a new antifungal antibiotic from aspergillus versicolor. 19684970688
[natural variability of the producer of amphotericin act. nodosus trejo in relation to antibiotic production]. 19684970744
[determination of biological activity of polysynthetic penicillins by the agar diffusion method]. 19684970746
detection of fusaric acid in the mycelium and conidia of fusarium oxysporum f. vasinfectum. 19684970752
dry heat or gaseous chemical resistance of bacillus subtilis var. niger spores included within water-soluble crystals.inclusion of spores of bacillus subtilis var. niger in water-soluble crystals increased the resistance of the spores to dry heat and to a gaseous mixture of methyl bromide and ethylene oxide. resistance of spores in glycine crystals to dry heat at 125 c was increased 5 to 24 times compared to unprotected spores. there appeared to be a positive correlation between the size of the crystal and the degree of resistance. the resistance to dry heat of spores included in sodium chloride crystals was ab ...19684970891
novel multi-slit large-volume air sampler.scientific investigators who are interested in the various facets of airborne transmission of disease in research laboratories and hospitals need a simple, continuous, high-volume sampling device that will recover a high percentage of viable microorganisms from the atmosphere. such a device must sample a large quantity of air. it should effect direct transfer of the air into an all-purpose liquid medium in order to collect bacteria, viruses, rickettsia, and fungi, and it should be easy to use. a ...19684970892
effect of cell moisture on the thermal inactivation rate of bacterial spores.thermal inactivation rates were determined for two strains of bacillus subtilis var. niger spores after equilibration to various relative humidity (rh) levels. in these tests, small thin stainless-steel squares were each inoculated with a drop of spore suspension and equilibrated to 11, 33, or 85% rh. following equilibration, the squares were placed on a hot plate preheated to 108, 125, 136, 164, or 192 c for various exposure times and then assayed for surviving organisms. the results revealed t ...19684970894
the sterilization of the respiratory circuits of automatic ventilators using formaldehyde vapour. 19684970963
screening tests for phenylketonuria. 19684971102
present status of different mass screening procedures for phenylketonuria. medical research council working party on phenylketonuria.the phenistix screening test for phenylketonuria in newborn infants, when used routinely at the recommended age of 4 to 6 weeks, passes as normal a substantial proportion-perhaps between a quarter and a half-of children with the disease, who are then diagnosed only after brain damage has occurred. screening procedures based on three other tests-namely, the paper chromatography test for o-hydroxyphenylacetic acid in urine, the guthrie test for phenylalanine in blood, and a modification of the gut ...19684971103
sedimentation of high molecular weight dna released from protoplasts of bacillus subtilis. 19684971120
the cell wall of bacillus subtilis during synchronous growth. 19684971160
temperature sensitive mutants of bacillus subtilis defective in dna synthesis. 19684971164
[studies on a somatic antigen isolated from a strain of b. subtilis]. 19684970313
[studies on the anti-inflammatory property of a somatic fraction of bacillus subtilis]. 19684970314
observations on the possible occurrence of transferase activity in alpha-amylase preparations. 19684970347
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