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[bacillus cereus: epidemiology, pathogenicity and pathogenesis. bibliographic review]. 19816798643
the effect of quaternary ammonium compounds and amine oxides on spores of bacillus cereus.the effect of 1-dodecylpiperidine 1-oxide and n,n'-bis(dodecyldimethyl)-1,2-ethane diammonium dibromide on the spores of bacillus cereus. particularly their binding to intact spores and spores with reduced cystine bonds, was investigated. the langmuir type of binding is involved in both cases. both compounds decreased the thermoresistance of spores. dpno decreased the fraction of non-germinating spores, the effect of the drug increasing with increasing concentration. this phenomenon was associat ...19816792009
[study of 7 disinfectants on different substrates]. 19816815775
effects of glass filtered solar radiation & of 2, 4 dinitrophenol on growth of bacillus cereus & on its survival after far-uv irradiation. 19816792061
fluorometric determination of tetracyclines in small blood and tissue samples.a reported fluorometric method to determine tetracycline in biological material was modified to determine low levels of the antibiotics when using only small amounts of starting material. the method is applicable to tissue distribution studies in small experimental animals and also can be used for serial determinations in blood samples of these animals and for biopsy material. the tetracyclines are extracted in the presence of ethylenediaminetetraacetic acid, and the phosphate precipitation step ...19816782190
the influence of temperature on the growth inhibitory effect of carbon dioxide on pseudomonas fragi and bacillus cereus.the growth inhibitory effect of 50 kpa (0.5 atm) co2 was tested for pseudomonas fragi in the temperature range 5-35 degrees c and of 101 kpa (1 atm) co2 on bacillus cereus in the range 18-46 degrees c. the maximum specific growth rate (mumax) of p. fragi in air (ph 6.7) was 0.44 h-1 at 35c, 0.66 h-1 at 30 degrees c, and 0.078 h-1 at 5 degrees c. in 50 kpa of co2 in air the relative inhibition of the growth rate was about 30% at 35 degrees c, 50% at 30 degrees c, and 90% at 5 degrees c. thus, the ...19816783284
mode of antibiotic action of 4-hydroxy-3-nitrosobenzaldehyde from streptomyces viridans.the free ligand, deferroviridomycin a, and its iron(ii) complex, viridomycin a, were detected in culture supernatant fluids of streptomyces viridans 1671 and were structurally characterized as 4-hydroxy-3-nitrosobenzaldehyde and tris(4-hydroxy-3-nitrosobenzaldehydato-n3,o4)ferrate(ii), respectively. we investigated the antibiotic activity of the above compounds and of the chemically synthesized bis copper(ii), tris cobalt(iii), and tris nickel(ii) complexes against escherichia coli nihj, salmone ...19816805420
[experience with the international standardization of the biological activity of the 3d standard for streptomycin].the activity of the third international standard of streptomycin was determined to be equal to 755 iu/mg with the use of accurately weighed amounts of streptomycin and 78037 iu with the use of streptomycin ampoules. the standardization center of the who decided to use the whole content of the ampoule of the third international standard of streptomycin, the activity of which is determined to be equal to 78500 iu per an ampoule.19816794439
mechanisms of sorbate inhibition of bacillus cereus t and clostridium botulinum 62a spore germination.the mechanism by which potassium sorbate inhibits bacillus cereus t and clostridium botulinum 62a spore germination was investigated. spores of b. cereus t were germinated at 35 degrees c in 0.08 m sodium-potassium phosphate buffers (ph 5.7 and 6.7) containing various germinants (l-alanine, l-alpha-nh2-n-butyric acid, and inosine) and potassium sorbate. spores of c. botulinum 62a were germinated in the same buffers but with 10 mm l-lactic acid, 20 mm sodium bicarbonate, l-alanine or l-cysteine, ...19816794451
inhibition of bacillus cereus spore outgrowth by covalent modification of a sulfhydryl group by nitrosothiol and iodoacetate.nitrosothiols with the general structure rsn==o were studied as a model system of bacteriostatic action toward outgrowing bacterial spores. with a taft plot analysis, the influence of the structure of the r group on the inhibitory effectiveness of a series of nitrosothiols showed that effectiveness as an inhibitor of bacillus cereus t outgrowth correlated with the electron withdrawal of r, but that size, shape, charge, hydrophobicity, and transportability had little influence. this was interpret ...19816795179
bacterial mesosomes: method dependent artifacts.the occurrence of mesosomes was investigated during septum formation of vegetative and sporulating cells of bacillus cereus. it has been demonstrated that bacterial mesosomes which are considered by numerous microbiologists as an integrated constituent of gram positive bacteria, are in reality artifacts arising during the preparation for electron microscopy. the conventional fixation methods allowed enough time for the cytoplasmic membrane to react to the changed conditions and to form the typic ...19816796029
bacterial spore components which enhance the bacteriostatic effectiveness of s-nitrosothiol.spore components exuded into the medium during outgrowth of bacillus cereus t enhanced the bacteriostatic effectiveness of s-nitrosomercaptoethanol, an inhibitor which prevents outgrowth at low concentrations and germination at higher concentrations. the enhancement effect was slight with respect to outgrowth, but dramatic with respect to germination, in that the inhibitory effectiveness of nitrosothiols toward germination inhibition was enhanced by as much as 33-fold when nitrosothiols was in t ...19816797352
alteration by heat activation of enzymes localized in spore coats of bacillus cereus.heat activation (70 degrees c for 20 min) resulted in alteration in structural proteins and enzymes found in bacillus cereus spore coats. the three notable changes were increased glycosylation of coat proteins, alteration in polypeptide pattern on sodium dodecyl sulfate - polyacrylamide gels, and an increase in free sh groups of proteins. about three polypeptides leaked out in small quantities from the spore coats during heat activation. the extraction of five spore coat associated enzyme activi ...19816786720
a new test of differentiation of bacillus cereus and bacillus anthracis based on the existence of spore appendages. 19816799753
[constructive and energy metabolism changes in the formation and germination of resting retractile forms of bacillus cereus].it has been found that, as soon as bacillus cereus vegetative cells yield resting refractile forms under the action of specific autoregulating factors, the incorporation of labeled precursors of the main cellular biopolymers and lipids stops almost entirely and the level of cell endogenous respiration abruptly decreases. when the refractile forms revert to the vegetative state and growth, the processes of biosynthesis and respiration are restored. the level of metabolism typical of vegetative ce ...19816799756
microbiology of frozen goat meat and toxin production by bacillus cereus isolated therefrom.microbial analysis of commercial samples of freshly frozen goat meat and those stored at - 12 degrees c for one week revealed high counts of aerobic bacteria, bacillus cereus, staphylococcus aureus and streptococci. these counts increased with storage. psychrophilic bacteria were higher in number than mesophiles. the counts of salmonella were zero cfu/g in both freshly frozen and stock frozen meat. no yeast or moulds were encountered. mouse tests of crude culture filtrate of b. cereus and prepar ...19816798783
bacillus cereus meningo-encephalitis in a pre-term baby. 19816801918
influence of curvature of response lines in antibiotic agar diffusion assays. 19816783664
the problem of bacillus species infection with special emphasis on the virulence of bacillus cereus.although bacillus cereus is an uncommon ocular pathogen, infection with it usually results in loss of the eye. although previous reports have emphasized endogenous infection, our recent experience indicates the importance of b cereus infection following trauma. management is hampered by ineffectiveness of current empirical antibiotic regimens. this microorganism is resistant to both the penicillins and the cephalosporins. although b cereus is susceptible to gentamicin, our studies indicate that ...19816798519
bovine mastitis caused by bacillus cereus. 19816794216
identification of the apparently essential lysine residues in phospholipase c (bacillus cereus).phospholipase c (bacillus cereus) contains two apparently essential and very reactive lysine residues that may be labelled selectively by pyridoxal 5'-phosphate [aurebekk & little (1977) biochem, j. 161, 159--165]. one of these lysine residues was found in the 25-amino acid n-terminal fragment liberated by cnbr digestion of the pyridoxal-labelled enzyme and identified as lysine-6. two of the labelled peptides isolated from the chymotryptic digest of pyridoxal-labelled enzyme contained proline, s ...19816796052
salt extends the upper temperature limit for growth of food-poisoning bacteria.inclusion of nacl into the growth medium raised the upper temperature limit of growth of the following organisms: staphylococcus aureus (two strains), salmonella senftenberg, s. typhimurium, escherichia coli, streptococcus faecalis, bacillus cereus, clostridium sporogenes, c. perfringens (two strains). the magnitude of the response varied with the culture, the largest being 3.5 degrees with b. cereus cells. the spores of b. cereus were not protected by salt but clostridial spores behaved as the ...19816796247
bioluminescent assay for lipase, phospholipase a2, and phospholipase c. 19816796804
[effect of starch and egg products on the development of bacillus cereus in milk]. 19816797030
[sensitivity of bacterial isolated from non-parenteral drugs to antibiotics. i. isolation and identification of microorganisms]. 19816797258
[sensitivity of bacteria isolated from non-parenteral drugs to antibiotics. ii. sensitivity of gram-positive strains]. 19816797259
[evaluation of the bactericidal activity of chiral alkylammonium salts derived from n-benzoyl-amino acids with escherichia coli and bacillus cereus. i. -- evaluation of the antibacterial activity (author's transl)].long-chained alkylammonium salts (c12 and c16) obtained from chiral n-benzoyl-amino acids were tested against escherichia coli and bacillus cereus, to evaluate their antibacterial efficacy. the bactericidal activity (mbc) determined by the membrane filtration technique and the bacteriostatic activity values suggested anionic group participation. the participation of the anion moiety, depended on nature and configuration of the amino acid. stereoselective interactions appeared between bacteria an ...19816795576
[evaluation of the bactericidal activity of chiral alkylammonium salts derived from n-benzoyl-amino acids with escherichia coli and bacillus cereus. ii. -- adsorption mechanism and interpretation (author's transl)].by testing the antibacterial activity of new long-chained alkylammonium salts with organic asymmetric anions (n-benzoyl-amino-acids) we have been able to precise the kind of interaction that would take place between the microorganisms and the two groups of the surfactant molecule. evidence for anion participation to the bactericidal activity appeared through kind and configuration of the amino acid. a mechanism is proposed, that takes into account, hydrophobic and ionic interactions with cation ...19816795577
[the importance of aerobic spore-forming bacillaceae in routine demonstration of microorganisms (author's transl)].according to our studies the number of spore-forming bacilli at the average amounts to 2 to 15% within the air and food flora. due to their presence in the air they can contaminate test samples. likewise it is to be taken into consideration that they can cause pathological changes in susceptible organisms. in our laboratory we isolated aerobic bacilli from biological material in seven cases whereby pathogenicity was established in five cases thereof.19816795855
bacillus cereus toxins. 19816792636
effect of some divalent metal cations on phospholipase c from bacillus cereus.incubation of phospholipase c from bacillus cereus with certain divalent metal cations caused enzyme inactivation with cu(ii) being particularly effective. the inactivation arose from the reversible exchange of zn(ii) in the enzyme with the metal cations. both zinc atoms in the enzyme exchanged rapidly with cu(ii) whereas only one exchanged spontaneously with co(ii). with lecithin substrates, cozn-phospholipase c had a specific activity of 3.6-11.3% of that of znzn-phospholipase c, whereas the c ...19816792844
microbial production of vitamin b12 antimetabolites. iv. isolation and identification of 4-keto-5-amino-6-hydroxyhexanoic acid.4-keto-5-amino-6-hydroxyhexanoic acid was isolated from bacillus cereus 102804 fermentations and found to inhibit the growth of gram-positive and gram-negative bacteria, when grown in a chemically defined medium. the mechanism appeared to be the inhibition of delta-aminolevulinic acid dehydratase. the ki value of 4-keto-5-amino-6-hydroxyhexanoic acid in an enzyme preparation of propionibacterium shermanii was 0.72 microm. similar test conditions with 4-keto-5-aminohexanoic acid resulted in ki of ...19816792174
effect of detoxin d on blasticidin s uptake in bacillus cereus. 19816786916
a note on the occurrence of bacillus cereus and other species of bacillus in indian spices of export quality. 19816788744
xeroderma pigmentosum cells treated with proteases to relax chromatin structure do not exhibit increased unscheduled dna synthesis. 19816789199
[severe intestinal diseases caused by aerobic bacterial spores]. 19816791090
bovine mastitis caused by bacillus cereus. 19816791370
[possible etiologic role of bacillus cereus in acute intestinal diseases of children]. 19816791411
[microbiological preservation effect of chlorhexidine acetate]. 19816791462
bacterial food-poisoning versus coliforms-faecal streptococci and gamma radiation survival/dose.all the species tested were sensitive to gamma radiation treatment, even at a dose as low as 0.001 mrad. the spore-former bacillus cereus (pcj 213) proved to be more resistant than the rest of the tested species, while the gram-negative organisms were more susceptible to irradiation treatment. the tolerance among those organisms is depicted by the descending order: proteus vulgaris, irregular vi, escherichia coli i, e. coli iii, and salmonella typhi-murium. yet all the tested faecal streptococci ...19816784377
bacillus cereus food poisoning. 19816786231
emetic food poisoning caused by bacillus cereus.symptoms of acute food poisoning developed in eight members of a group who ate lunch at a cafeteria. after brief incubation periods, all affected individuals complained of nausea and abdominal cramps. four persons promptly experienced vomiting. none of those affected was found to have fever and all recovered with 48 hours. epidemiologic investigation incriminated macaroni and cheese as a cause of the illness and samples of this food contained large numbers of bacillus cereus. previous outbreaks ...19816786233
biosynthesis of glycerol teichoic acid in bacillus cereus: formation of linkage unit disaccharide on a lipid intermediate. 19816786295
synthesis of bacillus cereus spore coat protein.the major structural protein of bacillus cereus spore coats was synthesized, commencing 1 to 2 h after the end of exponential growth, as a precursor with a mass of ca. 65,000 daltons. about 40% of this precursor, i.e. 26,000 daltons, was converted to spore coat monomers of 13,000 daltons each, perhaps as disulfide-linked dimers. the rate of conversion varied, being initially slow, most rapid at the time of morphogenesis of the coat layers, and then slow again late in sporulation, coincident with ...19816780522
separation of atp-dependent dnase to atpase and dnase.a 250-fold purified atp-dependent dnase from bacillus cereus has been separated to dna-dependent atpase i and ii and a dnase specific for single-stranded dna (ssdnase) by means of high resolution of deae cellulose chromatography. simultaneously with the separation of atpase and ssdnase, a decrease in atp-dependent dnase activity was observed. complete separation resulted in the total loss of atp-dependent dnase activity. reconstitution of atp-stimulated dnase activity was dependent on the ratio ...19816456634
enzymatic reduction of mercurous and mercuric ions in bacillus cereus.a strain of bacillus cereus, which can grow in nutrient broth containing 50 microm hgcl2, was isolated from soil. mercurous or mercuric ion dependent oxidation of reduced nadph was demonstrated in crude extracts of cells grown in nutrient broth containing 10 microm hgcl2. the properties of this mercuric reductase were similar to those of the enzymes from r factor bearing escherichia coli in substrate specificity, heat stability, requirement of sulfhydryl compounds, sensitivity to some heavy meta ...19816452192
procedure for assay of tobramycin in serum containing moxalactam.moxalactam was examined as a substrate for 11 beta-lactamases. hydrolysis was shown only with the beta-lactamase from bacillus cereus, a nonpathogenic bacterium. tobramycin was assayed in tobramycin-moxalactam mixtures by using this beta-lactamase.19816457559
enzymatic hydrolysis of immobilized sphingomyelin by three bacterial phospholipases c.through hydrophobic interaction, sphingomyelin was adsorbed to agarose beads containing octyl groups by a stepwise dilution procedure. this immobilized lipid was used as a substrate for three bacterial phospholipases c (e.c. 3.1.4.3.). the degradation with time of this substrate showed two different fractions of the substrate according to hydrolysing velocity in the early part of the time-curve when phospholipases c from bacillus cereus and clostridium perfringens were used. the early fractions ...19816274145
food borne disease. 19816271107
[a preliminary report on the indicator of the enterovirus in hospital sewage (comparative studies on the resistance of f2 coliphage, b. cereus spore and hbsag to chemical agents) (author's transl)]. 19816277578
induction and repression of enzymes involved in exogenous purine compound utilization of bacillus cereus.5'-nucleotidase, adenosine phosphorylase, adenosine deaminase and purine nucleoside phosphorylase, four enzymes involved in the utilization of exogenous compounds in bacillus cereus, were measured in extracts of this organism grown in different conditions. it was found that adenosine deaminase is inducible by addition of adenine derivatives to the growth medium, and purine, nucleoside phosphorylase by metabolizable purine and pyrimidine ribonucleosides. adenosine deaminase is repressed by inosin ...19816274419
unusual copper-induced sensitization of the biological damage due to superoxide radicals. 19816273398
studies on (k+ + h+)-atpase. iv. effects of phospholipase c treatment.(1) the total phospholipid content of a gradient purified (k+ + h+)-atpase preparation from pig gastric mucosa is 105 mumol per 100 mg protein, and consists of 29% sphingomyelin, 29% phosphatidylcholine, 28% phosphatidylethanolamine, 10% phosphatidylserine and 4% phosphatidylinositol. the cholesterol content corresponds to 50 mumol per 100 mg protein. (2) treatment with phospholipase c (from clostridium welchii and bacillus cereus) results in an immediate decrease of the phosphate content. up to ...19816272855
interaction of beta-lactamase of streptomyces cacaoi. ii. cp-45,899, izumenolide and cephamycins.inhibition of a beta-lactamase of streptomyces cacaoi by cp-45,899, izumenolide and cephamycins was investigated and compared with that of a beta-lactamase of bacillus cereus. s. cacaoi enzyme could not hydrolyze cp-45,899. instead, hydrolysis of benzylpenicillin by the enzyme was inhibited in the presence of cp-45,899. although inhibition increased gradually with time, the inhibition line produced by cp-45,899 with time less curved than that produced by clavulanic acid and ps-5. furthermore, pr ...19816273376
enzymatic hydrolysis by bacterial phospolipases c and d of immobilized radioactive sphingomyelin and phosphatidylcholine.an assay system for phospholipases c has been described with sphingomyelin immobilized to octyl-sepharose cl-4b as substrate. the immobilization procedure was further developed and used with [14 c-choline]-sphingomyelin and [14c-choline] phosphatidylcholine (lecithin). these immobilized radioactive phospholipids made the enzymatic assays easier to perform and made it possible to increase the sensitivity. furthermore, since release of the choline part instead of the phosphate part of the substrat ...19816274146
in vitro antimicrobial activity evaluation of cefodizime (hr221), a new semisynthetic cephalosporin.cefodizime (hr221) is a new alpha-methoxyimino cephalosporin developed by hoechst-roussel with a reported serum half-life of over 2 h. in vitro susceptibility studies showed that the cefodizime spectrum includes all those enterobacteriaceae, staphylococci, streptococcus spp., haemophilus spp., and neisseria spp. normally susceptible to cefotaxime (hr756) or ceftizoxime (fk749) or both. cefodizime was less active (two- to eightfold) than cefotaxime or ceftizoxime against some enteric species, but ...19816275785
a possible role of stimulus-enhanced phosphatidylinositol turnover: calcium-sparing effect of diacylglycerol in inducing phase separation of phosphatidyl-choline/phosphatidylserine mixtures. 19816266405
specificity of translation of spore polypeptides in bacillus in vitro systems.cell free extracts prepared from exponentially growing escherichia coli and bacillus cereus as well as from bacillus cereus at the end of exponential growth were optimized for various factors required for amino acid incorporation when programmed with bacillus ribonucleic acid. all three preparations synthesized glutamine synthetase antigen when ribonucleic acid from a bacillus subtilis strain that overproduces glutamine synthetase was added. the post exponential bacillus cereus extract, however, ...19816165330
bacillus cereus cross-infection in a maternity-unit. 19816175691
isolation and properties of pili from spores of bacillus cereus.structures whose morphology is identical to that of bacterial pili have been isolated from spores of bacillus cereus. the structures are absent from log-phase and sporulating cells. the pili are 6.8 nm in diameter, are of variable length, and appear to emanate randomly from the exosporium. examination of spores from 12 bacillus species showed that only those from b. cereus and b. thuringiensis have pili. although isolated spore pili were shown to be composed of protein, their subunit nature was ...19816109707
bacillus cereus meningitis in a patient under gnotobiotic care. 19816116070
regulation by lipids of plant microsomal enzymes: ii. lipid dependence of the nadh-cytochrome c reductase of potato tubers.microsomal membranes from potato tubers were treated with a phospholipase c extracted from bacillus cereus. a positive correlation could be observed between the hydrolysis of membranous phospholipids and the decrease of the nadh-cytochrome c reductase activity. addition of total lipid or phospholipid micelles to phospholipase c-treated microsomes partially restored the nadh-cytochrome c reductase activity, thus proving the lipid-dependence of this enzyme.198116661641
microbial transformation of quercetin by bacillus cereus.biotransformation of quercetin was examined with a number of bacterial cultures. in the presence of a bacterial culture (bacillus cereus), quercetin was transformed into two crystalline products, identified as protocatechuic acid and quercetin-3-glucoside (isoquercitrin).198116345844
ceftazidime, a pseudomonas-active cephalosporin: in-vitro antimicrobial activity evaluation including recommendations for disc diffusion susceptibility tests.the in-vitro qualities of ceftazidime, as compared to other beta-lactams and three other antimicrobials (amikacin, gentamicin and chloramphenicol), were evaluated in a multilaboratory, multiphasic study in the united states. a total of 12,986 recent clinical isolates were tested by reference dilution methods in six medical centres over 45-60 days. ceftazidime was superior to the comparison cephalosporin (cefamandole) and comparable in spectrum and activity to gentamicin against enterobacteriacea ...198119802985
regulation by lipids of plant microsomal enzymes: iii. phospholipid dependence of the cytidine-diphospho-choline phosphotransferase of potato microsomes.cytidine-diphospho-choline diacyl-glycerol phosphorylcholine phosphotransferase activity was demonstrated in potato (solanum tuberosum l.) microsomes and the incorporation of cytidine-diphospho[(14)c]choline into phosphatidylcholine was characterized by the time course of (14)c incorporation and the effect of microsomal protein concentration on choline incorporation.potato microsomes were progressively delipidated by treatments (2 min at 0 degrees c) with increasing amounts of phospholipase c fr ...198216662446
wet and dry bacterial spore densities determined by buoyant sedimentation.the wet densities of various types of dormant bacterial spores and reference particles were determined by centrifugal buoyant sedimentation in density gradient solutions of three commercial media of high chemical density. with metrizamide or renografin, the wet density values for the spores and permeable sephadex beads were higher than those obtained by a reference direct mass method, and some spore populations were separated into several density bands. with percoll, all of the wet density value ...19826285824
gram-positive bacteria-induced granulocytopenia and pulmonary leukostasis in rabbits.pneumococci have been shown to induce granulocytopenia and pulmonary leukostasis which might contribute to morbidity and mortality in pneumococcal sepsis. we studied whether other gram-positive species (groups a and b streptococci, staphylococcus aureus, bacillus cereus, and clostridium perfringens) could also induce these phenomena. rabbits were challenged with nonviable preparations of each species, and all five species induced profound granulocytopenia (mean decrease of 90%) and pulmonary leu ...19826286493
the inactivation of bacillus cereus 569/h bera-lactamase by 6-beta-(trifluoromethane-sulfonyl)amidopenicillanic acid sulfone: ph dependence and stoichiometry. 19826294040
transfer of bacillus thuringiensis plasmids coding for delta-endotoxin among strains of b. thuringiensis and b. cereus.the recently discovered high-frequency transfer of plasmids between strains of bacillus thuringiensis was used to study the genetic relationship between plasmids and production of the insecticidal delta-endotoxin crystal. three strains of b. thuringiensis transmitted the cry+ (crystal-producing) phenotype to cry- (acrystalliferous) b. thuringiensis recipients. agarose gel electrophoresis showed that one specific plasmid from each donor strain was always present in cry+ "transcipients." the size ...19826294667
interaction of beta-iodopenicillanate with the beta-lactamases of streptomyces albus g and actinomadura r39.the beta-lactamases of streptomyces albus g and actinomadura r39 are inactivated by beta-iodopenicillanate. however, in contrast with the beta-lactamase i from bacillus cereus, they also efficiently catalyse the hydrolysis of the inactivator; with the s. albus g enzyme, kcat. is larger than 25s-1 and the number of turnovers before inactivation is 515. with the a. r39 enzyme, kcat. is larger than 50s-1 and the number of turnovers before inactivation is 80. after hydrolysis of the beta-lactam amid ...19826299270
cholinephosphotransferase activities in microsomes and neuronal nuclei isolated from immature rabbit cerebral cortex: the use of endogenously generated diacylglycerols as substrate.a neuronal nuclear fraction (n1) and a microsomal fraction (p3) were isolated from homogenates of cerebral cortices of 15-day-old rabbits. a nuclear envelope fraction (e) was prepared from n1. to assay cholinephosphotransferase, diacylglycerols were first generated in the membranes of these subfractions using a phospholipase c (bacillus cereus) preincubation. with levels of endogenous diacylglycerols producing maximal specific cholinephosphotransferase activities, an activity ratio of 1:1:5 was ...19826288206
phospholipase c from bacillus cereus has sphingomyelinase activity.purified, electrophoretically homogeneous phospholipase c (plc) preparations can be separated into two peaks by isoelectric focusing in sucrose gradients. the main peak has an isoelectric ph of 6.6-6.8 and contains two zn2+ per molecule. the more acid peak (isoelectric ph about 6.2) contains about one zn2+ per molecule and has a markedly reduced specific activity which can be raised by adding zn2+. the purified enzyme has a low sphingomyelinase activity which coincides completely with the lecith ...19826291130
6-beta-(trifluoromethane sulfonyl)-amido-penicillanic acid sulfone: a potent inhibitor for beta-lactamases. 19826288463
molecular properties and kinetic studies on sphingomyelinase of bacillus cereus.a sphingomyelinase of bacillus cereus was purified to a homogeneous state (512 u/mg, 2200-fold) as indicated by sds-polyacrylamide gel electrophoresis and the molecular weight (23,300) was determined by sedimentation equilibrium. the enzyme contained loosely-bound magnesium atom. the addition of mg2+ accelerated the enzyme reaction regardless of substrates and their physical state. the addition of ca2+ also accelerated the enzyme reaction slightly, when water-soluble substrates, i.e., 2-hexadeca ...19826284239
variations on the staining method in quantitative indirect immunofluorescence assays for bacillus spores, and the use of fluorescein--protein a.quantitative indirect immunofluorescence assays for b. anthracis and b. cereus spores fixed on multispot microscope slides have been performed using a microfluorometer to measure the fluorescence of individual bacteria. a study has been made of variations of the indirect assay sequence, in which the washing operation between application of 1st and 2nd antibody types was omitted. in one modification the addition of the indirect antibody was deferred, and in the second the direct and indirect reag ...19826184416
evidence for the presence of subpopulations of arenicola marina coelomocytes identified by their selective response towards gram+ve and gram-ve bacteria.in arenicola marina (l.), in vitro attachment and phagocytosis of bacteria appears to be serum-independent and thus, non-specific. mixtures of gram+ve and gram-ve bacterial species (gram+ve/-ve, of two gram+ve (gram+ve/+ve) and of two gram-ve bacterial species (gram-ve/-ve)were overlaid on coelomocyte monolayers, in order to introduce competition for cell surface 'receptors'. an enhanced total phagocytosis was only recorded in the gram+ve/-ve mixtures in comparison with their controls. the resul ...19826175542
postoperative neurosurgical infections due to bacillus species.the cases of 2 patients with postoperative ventriculitis due to bacillus species bacteria are presented. bacillus licheniformis was isolated from one patient following removal of an intraventricular meningioma, and bacillus cereus from another patient following placement of a ventriculoperitoneal shunt. both isolates were resistant to a variety of antibiotics, but both were sensitive to gentamicin and chloramphenicol. these cases emphasize several points; (a) bacillus species, usually thought to ...19826758158
the inhibition of vegetative cell outgrowth and division from spores of bacillus cereus t by hen egg albumen. 19826802792
photometric immersion refractometry of bacterial spores.photometric immersion refractometry was used to determine the average apparent refractive index (n) of five types of dormant bacillus spores representing a 600-fold range in moist-heat resistance determined as a d100 value. the n of a spore type increased as the molecular size of various immersion solutes decreased. for comparison of the spore types, the n of the entire spore and of the isolated integument was determined by use of bovine serum albumin, which is excluded from permeating into them ...19826802796
bacterial spore heat resistance correlated with water content, wet density, and protoplast/sporoplast volume ratio.five types of dormant bacillus spores, between and within species, were selected representing a 600-fold range in moist-heat resistance determined as a d100 value. the wet and dry density and the solids and water content of the entire spore and isolated integument of each type were determined directly from gram masses of material, with correction for interstitial water. the ratio between the volume occupied by the protoplast (the structures bounded by the inner pericytoplasm membrane) and the vo ...19826802802
membrane-bound penicillinases in gram-positive bacteria.the penicillinases of bacillus licheniformis, bacillus cereus, and staphylococcus aureus are related in structure and cellular localization to one another more closely than they are to penicillinases from gram-negative organisms. in the latter, penicillinases are almost exclusively found in the periplasm, while the gram-positive bacteria retain a substantial proportion as hydrophobic membrane-bound forms. we recently showed (nielsen, j. b. k., caulfield, m. p., and lampen, j. o. (1981) proc. nat ...19826802832
bacillus cereus endocarditis involving a prosthetic valve.a rare case of "late onset' endocarditis due to bacillus cereus occurred in a 55-year-old man who had a carpentier-edwards heterograft valve. combination therapy with gentamicin and clindamycin was administered as suggested by testing of minimal bactericidal concentrations for these antibiotics. valve replacement was necessary because of a paravalvular leak. there was no recurrence after six weeks of therapy.19826803367
[proteolytic activity of conditionally pathogenic microorganisms causing food poisoning].a study was made of proteolytic activity of the microorganisms proteus and bac. cereus, as well as of infected extract from raw meat. subject to study were also the effects of the temperature, storage and microbial association on the production of exoproteases. the results indicate an increase in the proteolytic activity in the course of storage at room and decreased temperatures and a lowering of the soluble protein amount in the samples, which attests to an active process of spoiling. associat ...19826803437
specific chemical modification of the readily nitrated tyrosine of the rtem beta-lactamase and of bacillus cereus beta-lactamase i. the role of the tyrosine in beta-lactamase catalysis.the function of the hydroxyl group of the tyrosine residue readily nitrated by tetranitromethane (tyrosine-105) in the rtem plasmid-derived beta-lactamase (penicillinase; penicillin amido beta-lactam-hydrolase, ec 3.5.1.6) from e. coli and in bacillus cereus beta-lactamase i has been investigated by chemical modification methods. in the case of b. cereus beta-lactamase i the nitrated tyrosine can be acetylated by acetic anhydride without effect on beta-lactamase activity the nitrated tyrosine of ...19826803835
molecular analysis of the phospholipids of escherichia coli k12.phospholipids from escherichia coli k12 were converted to 1,2-diacylglycerols with phospholipase c from bacillus cereus. high-pressure liquid chromatography of 1,2-diacylglycerol p-methoxybenzoates on lichrosorb rp-18 using 2-propanol/acetonitrile (35:65) as eluant permitted separation of 14 molecular species. the main combinations of fatty acids were 1-16:0-2-16:1, 1-16:0-2-cyclo-17:0 and 1-16:0-2-18:1. positional isomers were not present. the 1,2-di-16:0 compound was present at a significant l ...19826803841
[formation of resting refractile cells in bacillus cereus as affected by an autoregulatory factor].extracellular membrane-active compounds were shown to induce the transition of vegetative cells in the producing culture into resting refractile forms. the forms are characterized by the absence of energy, biosynthetic and destructive processes, by the capability to remain viable for a long time, and by a peculiar ultrafine organization. the physiological state and the ultrafine organization of b. cereus cells were found to change under the action of the autoregulatory factor introduced in the c ...19826803113
interaction between a bacillus cereus spore hexosaminidase and specific germinants.a purified coat-associated hexosaminidase from spores of bacillus cereus was studied to determine whether it could promote germination of dormant spores. spores of a coat-deficient mutant as well as chemically extracted spores were used as substrate. both of these spore preparations responded poorly to most germinants. however, absorbance loss was accelerated when the hexosaminidase was added in the presence of l-alanine. enzyme alone was not effective. the addition of d-alanine inhibited comple ...19826801016
preparation of 15n-labeled l-alanine by coupling the alanine dehydrogenase and alcohol dehydrogenase reactions. 19826811316
synthesis and biological activity of some new 3,6-dinitro-1:8-naphthaloyl- and 3,6-diamino-1:8-naphthaloylamino acids and dipeptide derivatives.synthesis of a series of 3,6-dinitro-1:8-naphthaloylamino acids (ii-ix) and some of their corresponding methyl esters (x-xvi) and 3,6-diamino-1:8-naphthaloylamino acid derivatives (xxix-xxxvi) is described. coupling of 3,6-dinitro-1:8-naphthaloylamino acids with amino acid methyl ester hydrochlorides in dioxane-dmf-et3n medium using dcc method furnishes the desired 3,6-dinitro-1:8-naphthaloyldipeptide methyl esters (xvii-xxviii). most of the synthesized 3,6-dinitro-1:8-naphthaloylamino acids, es ...19826811471
[the enzymatic analysis of sphingomyelin in hdl (author's transl)].a simple method is described for the enzymatic determination of sphingomyelin in the apolipoprotein b-free supernatants prepared by precipitation of blood sera with phosphotungstate/mgcl2. the analysis is based on the enzymatic hydrolysis of sphingomyelin, by sphingomyelinase from b. cereus, into phosphorylcholine and n-acylsphingosine, and subsequent hydrolysis of phosphorylcholine by alkaline phosphatase. the choline formed is determined by choline kinase in an optical test. the results from t ...19826809883
structural changes induced by subinhibitory concentrations of fosfomycin on staphylococcus aureus and bacillus cereus.exposure of staphylococcus aureus atcc 6538 and bacillus cereus atcc 11778 to 1/2 mic fosfomycin for 3 and 6 h was studied by transmission and scanning electron microscopy. after 3 h, a loss of density and irregularity of shape in the septum was noted, while after 6 h a delayed separation of newly formed cells was observed in both species. in staphylococcus aureus this resulted in the formation of chains of intact cells and cells in lysis. in bacillus cereus, it produced filamentous forms with m ...19826810067
inhibition of bacillus cereus phospholipase c by univalent anions.the rate of phospholipid hydrolysis in erythrocyte ghosts by bacillus cereus phospholipase c was markedly decreased by the presence of nacl at concentrations between 25 and 200 mm. the inhibition seemed to be due to cl- and was unaffected by the type of cation present. the larger univalent anions such as hco3-, br-, cl-, no3-, cno- and i- seemed most effective, whereas the bivalent anion so42- was relatively ineffective at 0.1 m, as were acetate and formate. tris buffers at 0.1 m caused marked i ...19826810875
concentration of chlortetracycline in the blood of yellow-crowned amazon parrots fed medicated pelleted feeds. 19826807277
an outbreak of bacillus cereus food poisoning. 19826817420
[bacillus cereus strain 147: producer of tetramethylpyrazine]. 19826815634
[method for determining the optimum concentration of a disinfectant for its clinical use]. 19826815725
[synthesis and properties of the n-ethyl derivatives of carminomycin and rubomycin].n-monoethyl derivatives of carminomycin, rubomycin, 13-dihydrocarminomycin and 13-dihydrorubomycin were synthesized by condensation of their amino groups with acetic aldehyde in the presence of sodium boron hydride. the respective n,n-diethyl derivatives of the antibiotics were formed as by-products of the reaction. new compounds such as n-ethylcarminomycin, n,n-diethylcarminomycin, n-ethyl-13-dihydrocarminomycin, n,n-diethyl-13-dihydrocarminomycin, n-ethylrubomycin and n-ethyl-13-dihydrorubomyc ...19826814352
glyceride-cysteine lipoproteins and secretion by gram-positive bacteria.the membrane penicillinases of bacillus licheniformis and bacillus cereus are lipoproteins with n-terminal glyceride thioether modification identical to that of the escherichia coli outer membrane lipoprotein. they are readily labeled with [3h]palmitate present during exponential growth. at the same time, a few other proteins in each organism become labeled and can be detected by fluorography after sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total membrane proteins. we distingui ...19826811555
inactivation of beta-lactamase i from b. cereus 569/h with phenylglyoxal, an arginine-selective reagent. 19826818958
assessment of immunofluorescence measurements of individual bacteria in direct and indirect assays for bacillus anthracis and bacillus cereus spores. 19826819288
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