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[detection of virulence markers in clinical strains of yersinia].the dispersion of plasmid pyv associated virulence markers in 474 yersinia strains isolated from people has been studied. the ability to autoagglutination, calcium dependence of growth and the specific antigens were identified in 157 strains of traditionally pathogenic yersinia enterocolitica serovars 03, 09, yersinia pseudotuberculosis serovar i. they were not found in 223 strains of other 12 serovars of yersinia enterocolitica, in 40 strains of yersinia frederiksenii, yersinia kristensenii, ye ...19911857370
[the effect of a plague pathogen plasmid on lethality and immunogenicity].on the model of yersinia transconjugants (yersinia pestis, yersinia pseudotuberculosis, yersinia enterocolitica) carrying the conjugative cointegrates containing the 47 and 65 md plasmids from yersinia pestis the data were obtained on the different affects of the latter plasmids on the lethality and immunogenicity conferred by the host bacterial cells. the plasmid effects were drastically different during bacterial infection in mice or guinea pigs. the possibility of appearance of the recombinan ...19911857372
[the effect of the bacterial genome on the expression and behavior of the plasmid determining the ca2+-dependence of the plague pathogen].the conjugative cointegrate containing the 47 md plasmid of yersinia pestis has been transferred into the strains of the different yersinia (yersinia pestis, yersinia pseudotuberculosis, yersinia enterocolitica) and escherichia coli ca. there appeared in the populations of recombinant yersinia under the conditions of ca2+ deficit at 37 degrees c the cells coming into the stasis stage or dying. it was shown on the model of yersinia enterocolitica that bacterial lethality might be prevented by exc ...19911857373
[the heterogeneity of collection strains of yersinia pseudotuberculosis and their molecular biological properties].the capacity of y. pseudotuberculosis strains for disassociation with the appearance of s- and p-forms has been studied. strains 852 and 9547 show high stability in s-forms, their conversion into r-forms occurring at 40-42 degrees c. strain 6953 shows pronounced polymorphism and instability of its associations at different growth temperatures. strain 9532 exists in s- and r-forms which retain their stability during numerous subculturings at different growth temperatures and prolonged storage. th ...19911858469
[the nonspecific changes in the immune system to the administration of the membrane-ribosomal fraction of the causative agent of pseudotuberculosis].nonspecific changes in different elements of the immune system of animals under the action of y. pseudotuberculosis membrane-ribosomal fraction (mrf) with high protective potency have been studied for the purpose of the analysis of the preparation for immunological safety. mrf has been shown to produce no changes in humoral immune response to antigens of different nature or to enhance this response, to produce no essential effect on the intensity of the reaction of delayed hypersensitivity to sh ...19911858471
[structure of the o-specific polysaccharide chain of the yersinia pseudotuberculosis lipopolysaccharide (serovar ii c)].an o-specific polysaccharide has been isolated on mild acid hydrolysis of lipopolysaccharide from yersinia pseudotuberculosis serovar iic and shown to consist of abequose, d-mannose and 2-acetamido-2-deoxy-d-galactose residues in the ratio 0.8:3:1. from the results of acid hydrolysis, 13c nmr, methylation and periodate oxidation studies the structure of the repeating unit of the o-specific polysaccharide is deduced as follows: (formula; see text)19911863285
acute mesenteric lymphadenitis due to yersinia pseudotuberculosis lacking a virulence plasmid.a serotype 4a strain of yersinia pseudotuberculosis lacking the virulence plasmid pyv (pyv- strain) was isolated from the mesenteric lymph nodes but not from the stool or the appendix of a 10-year-old girl with a diagnosis of acute mesenteric lymphadenitis. microscopically, reticulocytic abscess and lymphadenitis were persent in the enlarged mesenteric lymph nodes. antibody against the isolate was detected in the serum. the isolate was negative for the presence of plasmid pyv and plasmid pyv-med ...19911864948
allergic fungal sinusitis: problems in diagnosis and treatment.although first described in 1983, allergic aspergillus sinusitis (aas) has yet to gain wide recognition among otolaryngologists and pathologists. we have treated three patients with a history of asthma, nasal polyposis, and recurrent pansinusitis who fit the description of allergic aspergillus sinusitis. histopathologically, mucinous material with abundant eosinophils and charcot-leyden crystals ("allergic mucin") is interspersed with fungal hyphae. immunologic characteristics include serum tota ...19911865728
different patterns of behavior produced by haloperidol, pentobarbital, and dantrolene in tests of unconditioned locomotion and operant responding.three motor-impairing drugs with different putative mechanisms of action (haloperidol 0.00, 0.075, 0.15, 0.30 mg/kg ip; pentobarbital 0.00, 4.5, 9, 12 mg/kg ip; and dantrolene 0.00, 5, 7.5, 10 mg/kg ip) produced strikingly similar patterns of dose-dependent attenuation in unconditioned locomotor behavior. however, the same drugs and doses produced highly divergent patterns of disruption when tested using different groups of rats in a food-rewarded operant task, which included both response initi ...19911876659
[the rheumatic complications of pseudotuberculosis in a newly arrived population in siberia]. 19911887407
[the comparative effectiveness of serological and microbiological studies in the etiological diagnosis of reactive arthritis].the information content of serological and microbiological studies was studied and compared in 72 patients suffering from enterogenous reactive arthritis. a high level of antibodies to intestinal yersinia was detected in 11.1% of the patients, positive coproculture in 8.3%. only one patient demonstrated at a time a high level of antibodies and isolation of intestinal yersinia coproculture. the causative agent could usually be isolated in patients with the symptoms of enterocolitis. it is assumed ...19911887408
[pathology of the gastrointestinal tract in patients with reactive arthritis and its possible role in the pathogenesis of the disease]. 19911887410
factors promoting acute and chronic diseases caused by yersiniae.the experimental system constructed with the medically significant yersiniae provides a powerful basic model for comparative study of factors required for expression of acute versus chronic disease. the system exploits the close genetic similarity between yersinia pestis, the etiological agent of bubonic plague, and enteropathogenic yersinia pseudotuberculosis and yersinia enterocolitica. y. pestis possesses three plasmids, of which one, shared by the enteropathogenic species, mediates a number ...19911889045
cytogenesis in the monkey retina.time of cell origin in the retina of the rhesus monkey (macaca mulatta) was studied by plotting the number of heavily radiolabeled nuclei in autoradiograms prepared from 2- to 6-month-old animals, each of which was exposed to a pulse of 3h-thymidine (3h-tdr) on a single embryonic (e) or postnatal (p) day. cell birth in the monkey retina begins just after e27, and approximately 96% of cells are generated by e120. the remaining cells are produced during the last (approximately 45) prenatal days an ...19911894769
identification and cloning of a fur regulatory gene in yersinia pestis.yersinia pestis is one of many microorganisms responding to environmental iron concentrations by regulating the synthesis of proteins and an iron transport system(s). in a number of bacteria, expression of iron uptake systems and other virulence determinants is controlled by the fur regulatory protein. dna hybridization analysis revealed that both pigmented and nonpigmented cells of y. pestis possess a dna locus homologous to the escherichia coli fur gene. introduction of a fur-regulated beta-ga ...19911898928
bacterial entry into eukaryotic cells. 19911905978
[study of the dependence of gene expression of plague pathogen plasmid 65md on the host bacterium genome].the conjugative cointegrate containing yersinia pestis 65 md plasmid coding for the production of traction i antigen and mouse toxin has been transferred into yersinia pseudotuberculosis, yersinia enterocolitica and escherichia coli cells. analysis of the transconjugants obtained has confirmed the connection of the genetical determinants for the mentioned bacterial products with the 65 md plasmid. the similar level of fra and tox-genes expression has been found in yersinia cells while fraction i ...19911906986
[antibacterial and therapeutic effectiveness of a pectin from sea grass zostera].the kinetics of the antibacterial activity of zosterin, a polysaccharide preparation of a sea grass belonging to zoster, was studied. by its chemical structure zosterin is a low ++methoxylated pectin. in vitro the preparation markedly inhibited the growth of ++gram-negative and ++gram-positive organisms: s. aureus, e. coli, y. pseudotuberculosis, s. typhimurium and ps. aeruginosa. on a model of experimental pseudotuberculosis++ infection caused by oral contamination of mice f1 (cba x c57b1) with ...19911909858
[study by nmr and circular dichroism of synthetic "zinc finger" peptide of viral gag protein from hiv-2].we report the synthesis in solid phase of an 18-amino acid peptide that contains the cysteine-rich region of the structural gag protein of hiv-2. the characterization of this fragment and of its interaction with zn2+ has been made by one- (1 d) and two-dimensional (2d) nmr techniques and by circular dichroism. our results suggest that in aqueous solution the complexation produces a significant perturbation in the conformation of this peptide.19911913257
muscarinic-receptor functioning in tracheas from normal and ovalbumin-sensitive guinea pigs.responses to bilateral vagal nerve stimulation, to field stimulation, and to exogenous methacholine and histamine were compared in tracheas isolated from (a) saline injected (i.p.) and saline-aerosol exposed guinea pigs (control), (b) ovalbumin-sensitized and saline-aerosol exposed (sensitized) guinea pigs, and (c) ovalbumin-sensitized and 2% ovalbumin-aerosol exposed (challenged) guinea pigs. tracheal pressor responses (cmh2o; 1 cmh2o = 98.1 pa) to nerve and field stimulation, and maximal respo ...19911913333
fusarium oxysporum: status in bioethanol production.fermentation of lignocellulosic materials to ethanol and other solvents provides an alternative way of treating wastes and producing chemical feedstocks and fuel additives. considerable efforts have been made in past 10 years to improve the process based on lignocellulosic biomass and hydrolysate that contains a complex mixture of sugars, decomposition products of sugars, and sometimes the inhibitory levels of soluble lignin. despite the relative abundance of d-xylose in crop and forest residues ...19911913845
[approach to evaluation of the indicators of yersinia infections in reactive and rheumatoid arthritis].a total of 140 patients with reactive arthritis due to yersinia infection were under observation for 2-10 years. a group of 104 patients with erosive rheumatoid arthritis served as control for analysis of the results of bacteriological and serological studies. the bacteriological analysis of the feces, urine, smears of the feces and blood were made repeatedly. as established the articular syndrome in reactive arthrosis has its specific features depending on the nature of the pathological process ...19911925260
yersinia pseudotuberculosis in a persian cat. 19911926722
wild animals as the source of infection with yersinia pseudotuberculosis in shimane prefecture, japan. 19911935073
the v antigen of yersiniae: an overview. 19911935079
renal insufficiency in mice experimentally infected with yersinia pseudotuberculosis. 19911935080
experimental infection of mice with yersinia strains bearing or not bearing the virulence-associated plasmid. 19911935082
adhesion of yersiniae to rabbit intestinal constituents: role of outer membrane protein yada and modulation by intestinal mucus. 19911935087
chromosomal marker for the 'high pathogenicity' phenotype in yersinia. 19911935090
molecular biology of yersinia pseudotuberculosis large plasmid pvm82. 19911935092
plasmid-mediated characteristics of yersinia pseudotuberculosis strains isolated in italy from humans, cattle and swine. 19911935093
preparation of monoclonal antibody to v antigen from yersinia pestis. 19911935094
cases of yersinia pseudotuberculosis infection having diagnostic criteria of kawasaki disease. 19911935106
several aspects of yersinia pseudotuberculosis infection in children. 19911935107
renal complications of yersinia pseudotuberculosis infection in children. 19911935108
yersinia pseudotuberculosis infection in the mountain area. 19911935109
yersinia pseudotuberculosis infection in children due to untreated drinking water. 19911935113
comparison of plasmid dna among different serogroups of yersinia pseudotuberculosis. 19911935117
intracellular targeting of the yersinia yope cytotoxin in mammalian cells induces actin microfilament disruption.pathogenic yersinia spp., including the etiological agent of plague, y. pestis, all carry a common plasmid that encodes a number of essential virulence determinants, the yop proteins. one of these, yope, has been shown to be involved in the obstruction of the primary host defense by a molecular mechanism leading to inhibition of phagocytosis (r. rosqvist, a. forsberg, m. rimpiläinen, t. bergman, and h. wolf-watz, mol. microbiol. 4:657-667, 1990). although the yop proteins are secreted into the c ...19911937815
image cytometry of progesterone receptor expression during the cell cycle in the mcf-7 cell line.progesterone receptors (pr) appear to be distributed in a heterogeneous way in mammary tumor cells. the study presented here was designed to examine if heterogeneity of pr expression is cell-cycle dependent. immunofluorescence techniques were used to label pr on the mcf-7 human breast cancer cell line and image cytometry was used to analyze the pr expression during g0 (ki-67 antigen-negative cells), g1, s, and g2/m cell-cycle phases. a second pr, brdu, and dna analysis was performed to study pr ...19911940323
[yersiniosis in a general hospital in the basque country (1984-1989). clinical and epidemiological aspects].the features of the diseases caused by yersinia have not been properly evaluated in the basque country. the present study was performed to assess the incidence and consequences of this infection.19911943291
purification and partial characterization of a soluble hemagglutinin from yersinia pseudotuberculosis.a soluble hemagglutinin (ha) produced by yersinia pseudotuberculosis strain inoue, serotype 5b, was purified by ammonium sulfate precipitation, gel filtration on sepharose cl-6b and high performance liquid chromatography on a deae-5pw anion-exchange column. the purified ha was a 14.5 kda protein with an isoelectric point of 4.5. amino acid analysis indicated that the ha consisted of 133 residues, corresponding to the molecular weight of 14,100. the amino acid sequence of n-terminal 38 amino acid ...19911943846
[postcoital uterine microbe colonization and endometritis in the mare].in the mare, natural breeding is associated with bacterial contamination of the reproductive tract. the purpose of this study was to examine postcoital bacterial contamination and the resulting inflammatory response of the uterus. uterine swabs for bacteriological and cytological examination were obtained from 80 mares. each mare was sampled once between 4 and 69 hours postbreeding. in mares which did not conceive, sampling was repeated at the following estrus. the findings were compared with th ...19911948985
a review of 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced changes in immunocompetence: 1991 update.2,3,7,8-tetrachlorodibenzo-p-dioxin, more popularly called dioxin or tcdd and referred to in this review as 2,3,7,8-tcdd, is considered the prototype of the polychlorinated dibenzo-p-dioxins (pcdd). the pcdd are true contaminants and are formed primarily as byproducts in the manufacture of materials requiring the use of chlorinated phenols and during the combustion of chlorinated chemical products. from an environmental perspective, the pcdd have been most closely associated with the use of a nu ...19911949050
[the immunoenzyme analysis of ceruloplasmin].a highly specific and sensitive enzyme immunoassay (eia) system, suitable for the qualitative analysis of ceruloplasmin, has been developed. the possibility of its use for the examination of children with mononucleosis and pseudotuberculosis has been studied. an increase in the concentration of ceruloplasmin has been more pronounced in infectious mononucleosis (0.506 +/- 0.026 g/l) and pseudotuberculosis (0.421 +/- 0.157 g/l). the results of eia coincided with the data obtained by radial immunod ...19911950269
[the detection of human pseudotuberculosis cases in volyn province from 1975 to 1989]. 19911950294
[cytological examination of urethral and endocervical smears in the diagnosis of infections].cytologic studies of smears from 130 women suffering from various diseases, including 61 pregnant patients with gestoses, have shown structural changes of the cells in the smears from the endocervix and urethra, that permitted the diagnosis of mycoplasma infection in 43.8% of patients, chlamydia infection, 52.3%, and herpes simplex in 72.3% of patients; in 63.9% of patients these infections were combined. cytologic studies may be useful in the tentative diagnosis of urogenital infections, includ ...19911951977
failure of beta-lactam antibiotics and marked efficacy of fluoroquinolones in treatment of murine yersinia pseudotuberculosis infection.the treatment of yersiniosis by beta-lactams is questionable considering the proven failure of newer beta-lactams for treating murine yersinia enterocolitica infection. another modality of experimental treatment was performed with a virulent strain of y. pseudotuberculosis (nonproducer of beta-lactamase) highly susceptible (in terms of mics) to amoxicillin, cefotaxime, ceftriaxone, imipenem, doxycycline, gentamicin, and ofloxacin. the in vivo comparative efficacy of these drugs was evaluated in ...19911952849
[bacterial ileocecitis, a "new" disease].786 patients with suspected acute appendicitis or appendiceal mass were examined by ultrasonography to distinguish appendicitis from bacterial enteritis. 533 of these patients were described before. in 91 (11.6%) ultrasonography revealed the characteristic picture associated with bacterial enteritis of the ileocaecal region-enlarged mesenteric lymph nodes and mural thickening of the terminal ileum and caecum--but no image of an inflamed appendix. in 64 of these a bacterial infection was confirme ...19911956444
mapping and topographic localization of epitopes of the yersinia pseudotuberculosis invasin protein.the yersinia pseudotuberculosis invasin protein is a 986-amino-acid outer membrane protein that promotes bacterial penetration into mammalian cells by binding to beta 1-chain integrin receptors. we previously showed that the integrin binding domain is encoded by the carboxyl-terminal 192 amino acids. to further investigate the structure of this protein, we characterized a set of 32 monoclonal antibodies (mabs) directed against invasin. invasin deletion derivatives and fusion proteins carrying di ...19911716609
an extended antigenic scheme for yersinia pseudotuberculosis. 19911718658
molecular cloning and genetic characterization of the rfb region from yersinia pseudotuberculosis serogroup iia, which determines the formation of the 3,6-dideoxyhexose abequose.the rfb region of yersinia pseudotuberculosis serogroup iia has been cloned and expression of o antigen in escherichia coli k12 was demonstrated. transposon mutagenesis analysis confined the dna region required for o antigen expression to a 19.3 kb fragment, and the o antigen expressed was visualized by sds-page and silver staining. southern hybridization analysis demonstrated significant levels of similarity between the yersinia rfb region and the 3,6-dideoxyhexose pathway genes rfbf and rfbg, ...19911724263
prevalence of antibodies to five selected zoonosis agents in monkeys.the prevalence of antibodies against 5 zoonosis agents was determined in serum samples of 443 breeding monkeys. of the monkeys, 296 were bred or kept for a long time at r institute, and the remaining 147 were newly imported from the philippines and kept at s institute for quarantine. antibodies to simian virus 40 were highly prevalent at 89.1% among monkeys of r institute, whereas no antibody could be detected in those of s institute. antibodies to chlamydia psittaci and yersinia pseudotuberculo ...19911657210
pulse field electrophoresis of the chromosome of the pathogenic yersiniae. 19911657518
[group disease in children caused by enteroviruses and yersinia]. 19911663297
proliferation of chicken lymphoblastoid cells after in vitro infection with marek's disease virus.activated, thymus-derived (t) lymphoblasts were exposed to marek's disease virus and cultivated in attempts to induce in vitro transformation. after 9 to 15 days, colonies or small clusters of proliferating lymphoblasts were observed in cultures from three of a total of 122 attempts. these developed into proliferating cell cultures that resembled conventional marek's disease (md) lymphoblastoid cell lines in terms of growth characteristics and morphology. all proliferative cultures were unusual ...19911664718
[phenotypic features of a strain of yersinia pseudotuberculosis with the pvm82 plasmid, detected at pseudotuberculosis outbreak foci].the phenotypic properties conferred to yersinia pseudotuberculosis cells by the genetical determinants of a 25md fragment of the plasmid pvm82 coding for the modified cellular immune response in the infected organism. the fragment was shown to determine the conjugative properties of the plasmid, the resistance of bacterial cells to a number of hydrophobic agents and cellular ability to absorb the congo red dye. the latter confirms the presence of additional structural components in the cell wall ...19911664911
correlation between cd4 cell counts and cellular and plasma viral load in hiv-1-seropositive individuals.we conducted a study of 152 hiv-1-seropositive individuals in order to evaluate the possible correlations between the isolation of hiv from peripheral blood mononuclear cells or from plasma and cd4 cell counts. hiv was isolated from only 36% of plasma samples, and the isolation rate was closely related to cd4 cell counts, increasing gradually from 0% in subjects with greater than 800 x 10(6)/l cd4 cells to 88% in those with less than 100 x 10(6)/l cd4 cells. in contrast, hiv was isolated from 92 ...19911676276
application of the polymerase chain reaction and immunofluorescence techniques to the detection of bacteria in yersinia-triggered reactive arthritis.leukocytes in synovial fluid and peripheral blood samples from patients with yersinia-triggered reactive arthritis were analyzed after dna amplification using the polymerase chain reaction. the primers applied were specific for the virulence plasmid-coded 1cre genes of yersinia enterocolitica o:3 and yersinia pseudotuberculosis iii. no yersinia dna was observed within the synovial fluid cells or peripheral blood cells by polymerase chain reaction techniques. however, yersinia antigens were detec ...19911984781
combined use of released proteins and lipopolysaccharide in enzyme-linked immunosorbent assay for serologic screening of yersinia infections.an elisa for the screening of serum antibodies to yersinia species was developed using plasmid-encoded released proteins of yersinia enterocolitica o:8 and lipopolysaccharide of y. enterocolitica o:3 as a combined antigen. of 43 sera from patients infected with one of six different yersinia serotypes, 40 (93%) were positive in this assay. when tested using six serotype-specific elisas with the corresponding yersinia bacteria as antigens, 38 (88%) were positive. this screening elisa detects antib ...19911988526
[yersinia pseudotuberculosis in new world monkeys].it is reported on four cases of pseudotuberculosis in 2 saimiri sciureus, 1 callithrix jacchus/penicillata hybrid and 1 s. oedipus. the animals came from two different groups, one being infected with y. pseudotuberculosis serotype i, the other with y. pseudotuberculosis serotype ii. all cases showed intestinal infection of thermophile campylobacter. remarkable were severe haemorrhagic components and the distinct rhs-proliferation, especially in the mesenteric lymphatic nodes.19912015031
role of the yersinia outer membrane protein yada in adhesion to rabbit intestinal tissue and rabbit intestinal brush border membrane vesicles.the yersinia virulence plasmid confers on strains of yersinia pseudotuberculosis and y. enterocolitica an adhesive potential superior to the one encoded by the chromosome alone. we have evaluated the role of the plasmid-encoded outer membrane protein yada (formerly called yopl) in adhesion. insertional inactivation of the yada gene (formerly called yopa), which encodes yada, led to a reduction in the capacity of plasmid-carrying strains of y. pseudotuberculosis 0:iii and y. enterocolitica 0:9 to ...19912018636
control of pseudotuberculosis in zoos. 19912021043
risk of yersinia infection among butchers.the purpose of this study was to evaluate the risk of yersinia infection among butchers. serum samples were collected from 146 abattoir workers, stratified into 3 groups according to exposure to swine throats and intestines. 100 healthy blood donors were used as controls. antibodies against yersinia enterocolitica o:3 and o:9 and y. pseudotuberculosis i and iii were measured using elisa. symptoms associated with yersiniosis were recorded in a questionnaire. antibodies against y. enterocolitica o ...19912028228
pseudotuberculosis at the zoological society of london (1981 to 1987) 19912028568
acute tubulointerstitial nephritis associated with yersinia pseudotuberculosis infection.two siblings infected with yersinia pseudotuberculosis suffered from acute renal failure about 2 weeks after the onset of the disease. renal histology in both siblings showed acute tubulointerstitial nephritis. yersinia pseudotuberculosis type vb was isolated from feces of one of them, antibodies to yersinia pseudotuberculosis type vb in their sera were elevated. the results of the present study suggest that acute renal failure complicating infections with yersinia pseudotuberculosis is due to a ...19912032394
zoonoses and other findings in hedgehogs (erinaceus europaeus): a survey of mortality and review of the literature.a survey of mortality in hedgehogs (erinaceus europaeus) was carried out between july 1976 and november 1986. most were from norfolk. of the 74 examined, 35 (47.3 per cent) were road casualties, one of which yielded salmonella typhimurium phage type (pt) 104. of the remaining 39, 13 (33.3 per cent) had salmonellosis due to s enteritidis pt 11. this organism, which appears to be common and widespread in hedgehogs in england was found in 10 separate incidents. the only other zoonosis was ringworm ...19912035215
[yersinia and campylobacter food poisoning]. 19912038688
efficacy and safety of recombinant human erythropoietin to prevent the anaemias of prematurity. european randomized multicenter trial. 19912040194
meiotic drive of t haplotypes: chromosome segregation in mice with tertiary trisomy.the properties of the t haplotypes, specific mutant states of the proximal region of chromosomes 17 in the house mouse, are of continuing interest. one such property is increased transmission of the t haplotype by heterozygous t/+ males to offspring. using the reciprocal translocation t(16;17)43h we have constructed males with tertiary trisomy of chromosome 17 (+t43/+ +/rb7+) carrying the robertsonian translocation rb(16.17)7bnr. only the progeny of these males which had inherited either t43/+ o ...19912040454
yersinia species isolated from sheep with enterocolitis.in 40 submissions to the regional veterinary laboratory (rvl) wagga wagga from sheep in southern new south wales from 1981 to 1989, 53 isolates of yersinia sp were recovered from 45 sheep in 37 flocks. of 53 isolates, 26 were identified as y. pseudotuberculosis, 20 as y. enterocolitica, 5 as y. intermedia and 2 as y. frederiksenii. twelve isolates of y. pseudotuberculosis tested in the slide agglutination test all belonged to serotype iii. the 20 y. enterocolitica isolates were categorised bioch ...19912043083
sir frederick hobday memorial lecture. all wind and water: some progress in the study of equine gut motility. 19912044514
fumonisin mycotoxins and equine leukoencephalomalacia. 19912045319
intestinal carriage of yersinia pseudotuberculosis by wild birds and mammals in japan.fecal specimens were obtained from wild birds and mammals in the eastern part of shimane prefecture, japan, an area where serotype 1b, 2b, 3, and 4b strains of yersinia pseudotuberculosis were found to be prevalent in humans. each of 869 animals, including 259 wild birds and 610 wild mammals, was screened for yersiniae. a total of 37 strains of y. pseudotuberculosis were isolated from 34 (5.6%) mammals, including 23 raccoon dogs (nyctereutes procyonoides), eight deer (cervus nippon), two hares ( ...19912059038
antibodies against dengue viral proteins in primary and secondary dengue hemorrhagic fever.antibodies against dengue viral proteins were demonstrated in sera from dengue-infected patients by polyacrylamide gel electrophoresis, western blotting, and enzyme immunoassay. primary dengue cases showed low titers of igg class antibodies to envelope (e) proteins and two non-structural proteins, ns3 and ns5, in sera collected during the convalescent phase. secondary dengue- infected patients always demonstrated igg antibodies to e proteins in sera collected during the acute phase, and high tit ...19912063952
[the possible mechanisms of the adaptation of microbial populations to low positive temperatures].biologically low temperature was studied for its effect on the survival of populations and their biochemical and molecular-genetical state. the research was carried out on yersinia pseudotuberculosis and shigella sonnei models. it is established that the process of low-temperature adaptation of the populations may occur either at the level of the whole population with participation of inducible cold isoenzymes or at the level of a part of population, the temperature being a selection factor of t ...19912067418
tyrosine phosphate hydrolysis of host proteins by an essential yersinia virulence determinant.the plasmid-encoded yoph protein is a protein-tyrosine phosphatase (ptpase; ec 3.1.3.48) that is essential for yersinia virulence. we have investigated the molecular basis for the role of ptpase activity in yersinia pathogenesis. allelic recombination was employed to introduce a defined mutation into the yoph plasmid gene. conversion of the essential cys-403 to ala in the catalytic domain of the protein abolished yoph ptpase activity and significantly reduced the virulence of yersinia pseudotube ...19911705028
analysis of the v antigen lcrgvh-yopbd operon of yersinia pseudotuberculosis: evidence for a regulatory role of lcrh and lcrv.virulent yersinia species possess a common plasmid that encodes essential virulence determinants (yops) which are regulated by the extracellular stimuli ca2+ and temperature. the v antigen operon was recently shown to be involved in the ca2(+)-regulated negative pathway (a. forsberg and h. wolf-watz, mol. microbiol. 2:121-133, 1988). we show here that the v antigen-containing operon of yersinia pseudotuberculosis is a polycistronic operon having the gene order lcrgvh-yopbd. dna sequencing analys ...19911705541
an o antigen can interfere with the function of the yersinia pseudotuberculosis invasin protein.escherichia coli strains harbouring the yersinia pseudotuberculosis inv gene are able to enter cultured mammalial cells. we show here that this property is not shared by all enteric bacteria, since shigella flexneri 2a cured of its virulence-associated plasmid and harbouring the inv gene is unable to enter mammalian cells efficiently. mapping studies showed that the region of the chromosome responsible for this phenotype includes rfab, a locus involved in the production of o antigen. s. flexneri ...19911710312
[the use of growth stimulants in the bacteriologic diagnosis of yersinia].the effects of k. s. karpuzidi's stimulants (0.5-1 percent) and of para-amino benzoic acid (0.1-05 mm) on the growth and multiplication of 3 yersinia pseudotuberculosis, 1 y. enterocolitica strains, and e. coli nonpathogenic strain were examined. karpuzidi's stimulant was found to selectively stimulate yersinia cell growth and inhibit escherichia development in conditions unfavorable for these bacteria. para-amino benzoic acid intensively stimulated the growth of both yersinia and escherichia. w ...19911710732
eric sequences: a novel family of repetitive elements in the genomes of escherichia coli, salmonella typhimurium and other enterobacteria.we describe a family of highly conserved, enterobacterial repetitive intergenic consensus (eric) sequences, 14 of which have been identified in escherichia coli and salmonella typhimurium and a further three in other enterobacterial species (yersinia pseudotuberculosis, klebsiella pneumoniae and vibrio cholerae). eric sequences are 126 bp long and appear to be restricted to transcribed regions of the genome, either in intergenic regions of polycistronic operons or in untranslated regions upstrea ...19911713281
[pharmaceutical education of nurses]. 1991258902
reversible and irreversible inhibition by anesthetics of the calcium-induced luminescence of aequorin.the actions of various kinds of so-called membrane-stabilizing drugs on ca2+-induced flash intensity of purified aequorin, a photoprotein obtained from aequorea aequorea, were examined. all drugs used in this experiment, including inhalational anesthetics, tetracaine, chlorpromazine, and morphine, depressed flash intensity, and the inhibition increased with time. the inhibition followed a mixture of reversible and irreversible kinetics. a method of analyzing irreversible kinetics is presented. t ...1991271977
[possibilities and limits of controlled clinical therapy studies for the demonstration of the effectiveness of antithrombotic therapy]. 199150982
[additional training for stoma nurses?]. 1991257928
research, a logical problem-solving method. 1991245269
lymphocyte ultrastructure in two cases of neuronal ceroid-lipofuscinosis.circulating blood lymphocytes from two patients with neuronal ceroid-lipofuscinosis (ncl) were investigated by transmission electronmicroscopy. ultrastructural examination showed two forms of intracytoplasmic single membrane-limited inclusions. contents of the first inclusion form were arranged in five distinct patterns: (1) granules, (2) membranous formations, (3) paracrystalline forms, (4) alternating electron-dense/electron-lucent arrangements, and (5) admixtures of these components. these mo ...1992205814
dental peer review: a conceptual model. 1992265032
on your mark--new assessment techniques for determining eligibility for midwifery registration. 1992257145
the vanishing male nurse. 1992257637
[application of nutrition knowledge by nurses graduated from the school of nursing of the são paulo university]. 1992257684
nurse could you care more? 1992257481
concerns of final semester baccalaureate nursing students.at 10 taped group meetings data pertaining to concerns of 19 final semester baccalaureate nursing students were collected. when data were categorized according to task-, self-, or client-related concerns, an important finding was the low frequency of client-related concerns. recommendations and suggestions for further research were made.1992257407
surgical-orthodontic correction of open-bite deformity.the combined simultaneous anterior and posterior maxillary ostectomy has proved to be a useful method of treating skeletal open-bite. it is indicated primarily in patients with lip incompetence, excessive exposure of maxillary anterior teeth, long lower-face height, contour-deficient chin, and class ii malocclusion. primary contraindications are the class iii skeletal open-bite and lip competence. we have used the procedure as routine treatment for many open-bites over the past 5 years since we ...1992265137
human platelet aggregation by yersinia pseudotuberculosis is mediated by invasin.plasmid-free strains of yersinia pseudotuberculosis induce aggregation of human platelets in vitro. it appears that this phenomenon is mediated by invasin (inv), a 103-kda outer membrane protein that permits bacteria to penetrate mammalian cells, since (i) an isogenic inv-deficient mutant failed to aggregate platelets compared with the parental strain; (ii) a monoclonal antibody directed against invasin inhibited platelet aggregation; (iii) inv+ escherichia coli hb101 promoted platelet aggregati ...19921730468
binding of cultured mammalian cells to immobilized bacteria.the invasin protein of yersinia pseudotuberculosis binds to integrin receptors on mammalian cells and promotes cellular penetration. we demonstrate here that the cell attachment activity of invasin can be detected in bacterial colonies that have been immobilized on filter membranes. invasin expressed in either escherichia coli k-12 or y. pseudotuberculosis mediated binding to membranes, and as few as 10(5) y. pseudotuberculosis resulted in detectable attachment of cultured epithelial cells. a si ...19921730504
isolation of yersinia enterocolitica serovar o8 from free-living small rodents in japan.yersinia species were isolated from 65 of 223 free-living small mammals trapped in 10 regions on honshu island in japan. of the 65 strains isolated, 1 was yersinia enterocolitica serovar o3, 8 were y. enterocolitica o5, 6 were y. enterocolitica o8, 3 were y. enterocolitica o9, and 1 was yersinia pseudotuberculosis 4b. of the six y. enterocolitica o8 strains, five were positive for autoagglutination, ca2+ dependence, and the 45-mda virulence plasmid and showed high pathogenicity for mice.19921734061
loss of the pigmentation phenotype in yersinia pestis is due to the spontaneous deletion of 102 kb of chromosomal dna which is flanked by a repetitive element.the pigmentation (pgm+) phenotype of yersinia pestis encompasses a variety of different physiological traits, all of which are missing in pgm- mutants. we have previously shown that loss of the pgm+ phenotype is accompanied by the spontaneous deletion of at least 45 kb of chromosomal dna, referred to as the pgm locus. using chromosomal walking, we have now mapped the full extent of the pgm locus in y. pestis strain kim6+. our results indicate that the locus spans 102 kb of dna which is absent in ...19921447977
[a new r-plasmid from yersinia pseudotuberculosis].yersinia pseudotuberculosis strain 140-p isolated from soil in the far east was found to harbour an r-plasmid different from the plasmids that had been isolated from the bacteria previously. a new r-plasmid pld140 is conjugation proficient and codes for the cellular resistance to streptomycin, tetracycline and sulfonamides. the plasid belongs to incompatibility group incp. its restriction endonucleases bamhi and sali profile is different from the ones of the plasmids belonging to the rp4 family.19921454082
structure of the proteolytic fragment f34 of calmodulin in the absence and presence of mastoparan as revealed by solution x-ray scattering.the solution x-ray scattering technique has been applied to examine the conformations of the proteolytic fragment f34 (78asp-148lys) of calmodulin in the absence of both ca2+ and mastoparan, in the presence of ca2+ only, and in the presence of both ca2+ and mastoparan. the radius of gyration and the molecular weight for the f34 fragment increased by 1.1 +/- 0.3 a and 19%, respectively, upon binding of both 2 mol of ca2+/mol to the f34 fragment and mastoparan to form the tertiary complex. a small ...19921457423
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