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essential virulence determinants of different yersinia species are carried on a common plasmid. 19817243971
[antibiotic sensitivity of strains of the plague microbe isolated from foci in the caucasus and transcaucasia].antibiotic sensitivity of 91 strains of the plague microbe isolated in armenia, azerbaijan, daghestan, georgia, kabardino-balkaria and the stavropol region in 1977-1979 from the rodents and their ectoparasites was studied with the method of serial dilutions in the hottinger agar. the following antibiotics were tested: streptomycin, erythromycin, tetracycline, methicillin, gentamicin, levomycetin, monomycin, lincomycin, ristomycin, rifampicin, kanamycin, doxycyclin and polymyxin m. all of the str ...19817259143
plasmids in yersinia pestis.pesticinogenic and ca2+-dependent strains of yersinia pestis harbored plasmids of about 6 and 45 megadaltons, respectively. in addition, most isolates examined possessed a cryptic 65-megadalton plasmid.19817216478
glyoxylate bypass enzymes in yersinia species and multiple forms of isocitrate lyase in yersinia pestis.isocitrate lyase and malate synthase, the two unique enzymes of the glyoxylate cycle, were detected in crude extracts of yersinia pestis, y. pseudotuberculosis, and y. enterocolitica. y. pestis, unlike escherichia coli and the other yersiniae tested, yielded two forms of isocitrate lyase during growth on acetate. these forms differed in electrophoretic mobility and temperature optima. one form (a) was present during growth on acetate, but was absent during growth on alternate carbon sources such ...19817462147
plague in a neonate.more than half of the cases of plague in the united states are diagnosed in children. in the 1970s, 105 cases were reported, a 350% increase over the 1960s. plague should be considered in the differential diagnosis of children from the western united states who have sepsis, especially those with lymphadenopathy. complications of plague include meningitis, metabolic encephalopathy, abscess, and pneumonia. with appropriate treatment, prompt clinical response usually occurs. careful isolation and e ...19817234765
[utilization and fermentation of 2-ketogluconate by "enterobacteriaceae" (author's transl)].two methods were compared: 1) the ability to use 2-ketogluconate (2-kg) as source of carbon and energy in defined medium, and 2) the ability to produce acid by fermentation of 2-kg (0.5 g/100 ml) in peptone water with phenol red. results of growth on, and acid production from, 2-kg were identical. a total of 990 strains was studied. no strain of escherichia coli, shigella (4 species), salmonella (4 sub-genera), yersinia pestis, y. pseudotuberculosis, edwardsiella (2 species), proteus vulgaris, p ...19817235456
[spread of yersinia among patients with acute intestinal infections].during 1979 a large-scale study of fecal samples obtained from patients with acute intestinal infections was carried out with the aim of establishing the presence of yersinia. as a result, 9 y. enterocolitica strains and 7 y. pseudotuberculosis strains were isolated. y. enterocolitica were classified with serovars o8, o3, and o5b and with biovars 2, 3, and 4; y. pseudotuberculosis were classified with serovars 1 and 3. the isolation of yersinia facilitated the identification of the etiological s ...19817269921
plague in brazil during two years of bacteriological and serological surveillance.in north-east brazil, where plague infection is endemic, bacteriological and serological methods were employed during a two-year period to determine accurately the occurrence of human infection and to define certain epidemiological features of both human and rodent infection. there were 67 confirmed cases of plague, predominantly among males and children. most of these cases occurred in two inland rural plateaux of ceara state. clustering of cases in place and time was common, as was a history o ...19816976228
rapid diagnostic test that uses isocitrate lyase activity for identification of yersinia pestis.the presence of high levels of isocitrate lyase activity in yersinia pestis grown on blood agar base medium, as compared with low levels of this enzyme in yersinia pseudotuberculosis and yersinia enterocolitica, suggested that the differences in the levels of this enzyme could be used for the presumptive identification of y. pestis. a modified, semiquantitative assay for isocitrate lyase activity is described which requires no expensive instrumentation, utilizes readily available chemicals and s ...19817014615
delayed-type hypersensitivity and acquired resistance to plague in mice immunized with killed yersinia pestis and immunoregulators.a simple reproducible footpad assay of delayed-type hypersensitivity (dth) to yersinia pestis was studied in mice immunized with 5 x 10(7) heat killed (hk) y. pestis alone or in conjunction with freund's complete adjuvant (fca), cyclophosphamide (cy), bcg, or both cy and bcg. the footpad reaction elicited with 5 x 10(6) hk y. pestis was shown to peak at 24 hr, was transferable with immune t lymphocytes but not with serum and had the classical dth histology, the highest level of dth occurred 6 or ...19817028602
[characteristics of the cytomorphological and other indices of immunogenesis in guinea pigs vaccinated after the preliminary administration of an erythropoiesis stimulant]. 19817199240
mutations to tolerance and resistance to pesticin and colicins in escherichia coli phi.the universal colicin-indicator strain escherichia coli phi, unlike e. coli strain k-12, is sensitive to pesticin, a bacteriocin produced by wild-type yersinia pestis. eleven distinct phenotypes of e. coli phi mutants were obtained by selection for insensitivity to pesticin, group b colicins, the group a colicin s4, or coliphage t5. representative isolates from eight of these classes closely resembled resistant receptor mutants (cir-, fep-, and tona-) or tolerant mutants (tonb-, exbb-, exbc-, iv ...19817012131
[persistence of l-forms of plague bacteria in experimental animals]. 19817018126
properties and pharmacological action of plague murine toxin. 19817025029
[isolation and purification of the chloramphenicol-acetyltransferase from y. pestis ev cells with extrachromosomal resistance to the antibiotic by affinity chromatography].purification of chloramphenicol acetyltransferase isolated from the cells of y. pestis ev-r with extrachromosomal resistance to chloramphenicol included ultrafiltration on the microporous membranes diaflo-xm 300 and biospecific chromatography on columns with cepharose 4b covalently bound with the reduced chloramphenicol. elution of chloramphenicol acetyltransferase was achieved by means of addition of native chloramphenicol to the buffer solution. such native chloramphenicol had higher affinity ...19816938164
[immunostimulating properties of an extract isolated from aloe vahombe. 2. protection in mice by fraction f1 against infections by listeria monocytogenes, yersinia pestis, candida albicans and plasmodium berghei].a partially purified extract of leaves of aloe vahombe, a plant endemic in the south of madagascar, administered intravenously to mice, protects them against infection of bacteria (listeria monocytogenes, yersinia pestis), parasites (plasmodium berghei) and fungus (candida albicans). the protective fraction must be administered two days before inoculation of the pathogenic agent. these results significantly confirm those we obtained in earlier study on mice infection by klebsiella pneumoniae. cu ...19817044327
[serum antibody and splenocyte immunoglobulin receptor affinity in multiply immunized mice].in the process of repeated immunization of cba mice with soluble pasteurella pestis capsular antigen the concentration of antibodies in the blood increased, but the dynamics of antibody affinity in the serum did not correlate with this process. for the first time the average splenocyte receptor affinity was measured, and this characteristics was found to exceed that of the corresponding serum antibodies. to explain the heterogeneity of antibodies, two hypotheses have been proposed. one hypothesi ...19816895274
human plague -- texas, new mexico. 19816789063
[serologic affinity and specificity of action of pseudotuberculosis and coli-dysentery phages].the study of serological properties, specificity and the range of action has revealed affinity between y. pseudotuberculosis phages (pst, 3m, kotlyarova, 2344, 2391), some coliphages (t2, t3, t4) and sh. dysenteriae phage (dd iv). the existence of serovar iii of y. pseudotuberculosis phages has been established; to this serovar phage pst belongs. newly isolated 2344 and 2391 belong to serovar i. the problem of the existence of y. pseudotuberculosis phages as an independent group is discussed.19817023155
[gamma glutamyltransferase activity in the genus "yersinia" (author's transl)].the expression of gamma-glutamyltransferase activity was studied among 87 strains representing all the yersinia species. y. pestis lacked constantly this enzyme, while y. pseudotuberculosis expressed it as a rule. this test, when positive, is useful for the exclusion of a suspected y. pestis diagnosis. all the other yersinia species, like most of the enterobacteriaceae, showed a gamma-glutamyltransferase activity.19816112942
virulence-associated plasmids from yersinia enterocolitica and yersinia pestis.a 44-megadalton plasmid associated with virulence and ca2+ dependence from yersinia enterocolitica 8081 was compared at the molecular level with a 47-megadalton plasmid associated with ca2+ dependence from yersinia pestis ev76. the plasmids were found to share 55% deoxyribonucleic acid sequence homology distributed over approximately 80% of the plasmid genomes. one region in which the plasmids differed was found to contain sequences concerned with essential plasmid functions. forty-five mutants ...19816273385
[affinity of serum antibodies and splenocyte receptors in immunized cba mice]. 19816171127
[response, to polymyxin, of plague microbe strains isolated from various natural foci and of their mutants with a decreased requirement in nutritional factors].investigation of the response to polymyxin of 65 strains of pasteurella isolated from various foci and 46 their back mutants showed that all of them were usually highly resistant to polymyxin (mic 200--500 micrograms/ml). the pasteurella strains isolated in the gissaro-darvaz natural focus, turkey and congo were highly sensitive to polymyxin (mic 10--25 micrograms/ml). single cultures highly sensitive to the antibiotic were detected among the polymyxin-resistant strains. polymyxin-sensitive muta ...19816271044
lesions resembling caries in unerupted teeth.two cases are presented documenting the recognition and treatment of defects in the coronal portion of unerupted mandibular second permanent molars. the teeth were surgically exposed and the lesions treated as deep pit and fissure caries. small, vital pulp exposures were encountered in both cases. calcium hydroxide was placed and restoration with amalgam followed. normal root formation and eruption occurred for 24 and 6 months, respectively. a firm diagnosis of caries, external resorption, or in ...1981273855
the role of play in paediatric nursing. 1981243751
angioplasty of a totally occluded right coronary artery.we have presented a case of angioplasty of a chronically occluded right coronary artery. the occlusion had been present for 6 wks by clinical estimates. the length of the occluded segment (approximately 55 cm) did not preclude a successful outcome. proper selection and manipulation of angioplasty equipment are, as in every case, critical for procedural success. subintimal guidewire passage, though a frequent event, is occasionally associated with ischemic manifestations, and mandates detection a ...19811555228
the effect of hycanthone and maleic hydrazide on the frequency of micronuclei in the bone-marrow erythrocytes of mice.male swiss mice were assigned to 6 groups of either 3 or 4 animals each. 3 groups were given hycanthone methanesulfonate intraperitoneally, at 40, 80 or 120 mg/kg, respectively; the dose was repeated after an interval of about 24 h. at the same time 2 groups received maleic hydrazide at 100 or 200 mg/kg, and the remaining group was given dimethyl sulfoxide which was used as a solvent for both drugs. 6 h after the second injection, the mice were killed and bonemarrow preparations were made. hycan ...1981351389
gene therapy: into the home stretch. 19812396099
opiate receptors: some anatomical and physiological aspects.it is possible to localize opiate receptors by light microscopic autoradiography using techniques developed for the autoradiographic localization of small diffusible molecules. the receptors are found in the neuropil of anatomical areas associated with various physiologic functions known to be altered by opiates. in some areas, opiate receptors are associated with the axons of small diameter fibers. many kinds of studies, especially electrophysiologic studies, provide evidence that receptors rev ...1981217293
[point of equivalence in the precipitation reaction and its use for determining antibody concentration and antigen valency]. 19826186107
[3':5'-cyclonucleotidephosphodiesterase in yersinia pestis ev]. 19826289056
[trial use of polymyxin and erythromycin for isolating cultures of the plague microbe in the kyzyl-kum].it is recommended that the hottinger agar medium with polymyxin or erythromycin be used for isolation of pure cultures of the plague microbe from specimens containing various species of microorganisms, except proteus. such a procedure was used in the kizil-kum for the bacteriological examinations with respect to plague. the procedure may be used in the natural foci with the strains resistant to these antibiotics.19826295257
[determination of antibody affinity by using antigenic and antibody immunosorbents].to determine the affinity of the active centers of antibodies, cellulose immunosorbents for antibodies and antigens have been used. the fixation of serum proteins on the sorbent, the interaction of fixed antibodies with a monovalent antigen and the graphic analysis of the results thus obtained allows one to assess not only the concentration of the effective active centers on the sorbent, but also all known characteristics of antibody affinity: the average association constant k0, the common asso ...19826184912
[rapid method of determination of structural characteristics of lipopolysaccharide by the presence of aldoheptose in pasteurella pestis and pseudotuberculosis bacteria]. 19826185755
[epidemiologic and hygiene significance of yersinia pestis and yersinia enterocolitica]. 19826213893
[experience in the comparative evaluation of the population sensitivity of mammals to the plague microbe based on the results of an epizootiological survey].an essentially new method for the evaluation of the population susceptibility of mammals to pasteurella pestis without the experimental infection of the animals is proposed. this method consists in using the conjugate result of the mass bacteriological and serological survey of plague carriers (the ratio of the number of infected animals to that of the animals having had the disease). the susceptibility of 12 rodent and martes species has been studied with the use of this criterion on the basis ...19826214127
[reproducibility of the results of determining antibody and antigen activity in the takachi microtitrator]. 19826181308
[rhamnose and melibiose cleavage by the causative agents of pseudotuberculosis]. 19826181321
[monthly dynamics of the antitoxic immunity against diphtheria and tetanus and of normal antibodies to fraction i of the plague microbe in adults].monthly characteristics of antitoxic immunity to diphtheria and tetanus toxoids in 3,334 adults in ryazan were determined. as a result, the following differences were established: the characteristics of antidiphtheria immunity were somewhat higher in the autumn-winter period and dropped to the minimal level in the winter-spring period; the maximal characteristics of antitetanus immunity clearly coincided with the hot season, its minimal characteristics with the cold season. for the first time th ...19826211009
rna synthesis in yersinia pestis during growth restriction in calcium-deficient medium.yersinia pestis requires 2.5 mm ca(2+) for growth at 37 degrees c but not at 26 degrees c. after a shift from 26 to 37 degrees c in a ca(2+)-deficient medium, an ordered series of metabolic alterations occur which result in transition from a growing cell to a viable but non-proliferating cell. the earliest known alteration in normal metabolism associated with this transition is a termination of net rna synthesis. competitive rna/dna hybridizations with uniformly labeled rna and stable rna compet ...19826174497
localization in yersinia pestis of peptides associated with virulence.an avirulent guanine auxotroph of wild-type yersinia pestis was used to select isogenic mutants lacking invasive determinants of virulence including v and w antigens (vwa-), genetically linked fibrinolysin, coagulase, and pesticin activities (pst-), and the capacity to absorb exogenous pesticin and pigments including hemin (pgm-). after growth in environments known to favor expression of these factors by the parent, cells were converted to spheroplasts and disrupted to obtain preparations of cyt ...19826210636
plague: review of ecology.yersinia pestis, the organism which causes plague in man and animals, circulates from host to host in nature by means of one of many flea vectors. there are about 340 mammals known to be susceptible to plague and over 30 fleas which are known to carry the organism. the habits of flea and host, usually a rodent, must to some extent coincide before infection can be established, and these habits are affected by the temperature and humidity of the environment, by vegetation cover, by season, indeed ...19826765301
[intestinal yersinia infection: pathogenesis, clinical course, epidemiology and diagnosis].human abdominal yersiniosis is caused by yersinia enterocolitica and yersinia pseudotuberculosis. the organisms invade the intestinal wall and produce an inflammation of the gut and the mesenteric lymph nodes. their virulence appears to be determined by a group of plasmids of 40-48 megadaltons molecular size, which stimulate the production of v and w virulence antigens and mediate the capacity of y. enterocolitica to adhere to, and invade into, epithelial cells.19826757111
comparison of passive haemagglutination and enzyme-linked immunosorbent assay for serodiagnosis of plague.sera of 42 suspect plague cases from ovamboland, namibia, were examined. elisa proved more sensitive than passive haemagglutination for the detection of f1 antibody and increased positive serodiagnoses of plague by 7-42%.19826758973
serologic survey for certain zoonotic diseases in black bears in california.black bears (ursus americanus) from 3 geographic areas of california were tested for antibodies against agents of 6 zoonotic diseases: toxoplasmosis (indirect hemagglutination), q fever (microagglutination), trichinosis (latex particle agglutination), botulism (passive hemagglutination), leptospirosis (plate agglutination), and plague (enzyme-linked immunosorbent assay). of 149 sera tested, 40 (27%) were positive for toxoplasma gondii antibodies and 25 (17%) had antibodies against coxiella burne ...19827174445
[9 epidemic cases of bubonic plague in tananarive (madagascar)]. 19827154899
effect of exogenous nucleotides on ca2+ dependence and v antigen synthesis in yersinia pestis.cells of yersinia pestis strain ev76 are known to cease growth after a shift from 26 to 37 degrees c in neutral ca2+-deficient medium; this effect is potentiated by mg2+. with 2.5 mm mg2+ and no added ca2+, restriction was relaxed at elevated ph at which maximum cell yields occurred in the presence of 20 mm exogenous atp. this atp-dependent growth was inhibited by ca2+ or 20 mm mg2+; the nucleotide was neither transported into the organism nor hydrolyzed extracellularly. with strain ev76, atp co ...19827152680
the first outbreak of human plague in lushoto district, north-east tanzania.a one-week investigation was performed at mkunki and mavumo (ward of shume, lushoto district, tanzania) at the time of outbreak (june, 1980). rodents, people, domestic dogs and fleas were examined, 36 rats, mostly rattus rattus, were caught and used in serological and bacteriological analysis for plague. 48 fleas, mostly xenopsylla brasiliensis, were collected from the captured rodents. 413 people and 11 dogs were examined. all rodent, carnivorous and human sera were tested for plague antibodies ...19827101402
[experience using fraction i of the plague microbe for revaccinating experimental animals].experiments on guinea pigs have shown that a pronounced revaccination effect develops in the animals receiving the booster injection of fraction i of pasteurella pestis 1.5--4 months after the primary immunization with live plague vaccine, while the booster injection of live plague vaccine produces a low revaccination effect due to the fact that this vaccine is badly adapted in the body after the primary immunization.19827102182
[production of pesticins on synthetic nutrient media].it was found that 96.2 per cent of the plague microbe cultures studied had a capacity to produce pesticin i on solid and liquid minimum synthetic nutrient media providing growth of the cultures. no pesticin iii was detected in the spheroplasts of plague microbes, when they were grown on the synthetic nutrient medium, while on the complete nutrient medium the inhibition zones were observed which was indicative of pesticin iii synthesis. the average value characterizing the titer of pesticin i pro ...19827125618
disc electrophoresis of phenol:acetic acid:water-soluble proteins for the identification of yersinia species.disc electrophoresis of phenol:acetic acid:water extracts of yersinia pestis, y produced pseudotuberculosis, y enterocolitica, y philomiragia and y ruckeri protein separation patterns characteristic of each species. the patterns produced by y pestis, y pseudotuberculosis, y enterocolitica and y ruckeri showed features in common. those given by y philomiragia strains were quite distinctive and showed little similarity to the other yersinia species. all y enterocolitica strains produced similar pa ...19827134648
[histopathology of yersinia pestis infection in rodents from plague foci in northeastern brazil]. 19827144551
solid-phase radioimmunoassay for detection of plague antigen in animal tissue.a rapid and sensitive radioimmunoassay for the detection of soluble yersinia pestis fraction i antigen using antibody-coated beads and radiolabeled immunoglobulin was applied to spleen-liver extracts, rendered noninfectious by either treatment or filtration, from rodents dead of plague. the procedure was capable of detecting nanogram amounts of soluble plague antigen.19827153344
evaluation of a qualitative isocitrate lyase assay for rapid presumptive identification of yersinia pestis cultures.qualitative rapid tests for isocitrate lyase activity were positive for all of 1,695 yersinia pestis cultures tested but for none of 382 cultures of other gram-negative species. the test is simple, required only commonly available, easily prepared reagents, and provided clear-cut positive or negative results within 20 min.19827050154
peripatetic plague.cases of plague continue to occur in various parts of the world, including the western united states, where plague is endemic among the wild rodent population. in 1980, a case of plague acquired in new mexico and hospitalized in nebraska illustrated the problem of plague occurring in persons traveling from the state in which they become infected to another state. nine cases of plague in travelers were identified among the 166 cases of plague reported in the united states from 1950 to 1980. physi ...19827053441
temperature-modulated immunogenicity to yersinia pestis from yersinia enterocolitica o3.the ability of yersinia enterocolitica o3, grown at 25 degrees c, to promote cross-immunity to y. pestis was lost after repeated subcultures at 37 degrees c, which selected for bacterial populations having lower in vivo survival. subculturing y. enterocolitica o3 from 37 to 25 degrees c restored the cross-immunogenicity although the in vivo survival remained low.19827076305
[erythrocyte tanning and the characteristics of protein sensitins]. 19827080743
[pesticinogenicity and pesticin sensitivity of pasteurella pestis strains isolated in the gornyi altai region]. 19827090656
experimental yersinia pestis infection in rodents after intragastric inoculation and ingestion of bacteria.to clarify the pathogenesis of oral plague infection, we studied the susceptibility of three species of rodents to intragastric inoculation of yersinia pestis, described the pathology and progression of infection, and measured antibody responses to fraction ia antigen of y. pestis. the 50% lethal doses of bacteria by intragastric inoculation for mus musculus, zygodontomys pixuna, and rattus rattus were log10 = 6.32, 5.46, and 9.62, respectively, which were at least 1,000-fold higher than the val ...19827095845
[effect of the cultivation temperature on the enzymes of sialic acid metabolism in the plague microbe]. 19827154995
[comparative evaluation of methods of obtaining phagolysates modelled on d'herelle's plague microbe phage].various methods for phage multiplication have been tested: the method of cellophane membranes, the method of combined media, the method of forced aeration of a liquid medium, growth at suboptimal temperature and growth at optimal temperature under stationary conditions. the phage in a high concentration (n x 10(10) plaque-forming units per ml) can be obtained by any of these methods with the exception of the last one yielding lower (by 1 or 2 orders) effectiveness of multiplication. the main fac ...19827158159
[isolation of l-form of yersinia pestis from wild rodents in an epizootic focus].four y. pestis cultures with unbalanced growth and 15 y. pestis cultures in unstable l-forms were isolated from wild rodents rhombomys opimus in the vicinity of lake balkhash. morphologically they were mainly represented by granular, spheroplast-like and filiform elements. when stained with luminescent serum, they acquired specific fluorescence; in indirect hemagglutination they yielded positive result. electron microscopy revealed the presence of morphological elements characteristic of the pro ...19827180264
advantages and use of laboratory-reared african multimammate mice (mastomys coucha) in the diagnosis of plague. 19827181544
[the plague bacillus. i. kinetics of growth]. 19827186794
surveillance of sylvatic plaque in oregon by serotesting carnivores.in oregon, during 1974-1979, 10.3 per cent of 3.255 statewide blood samples from carnivores were positive for yersinia pestis. the per cent positive rate and geometric mean positive titer increase monthly from january (6.5 per cent) to june (21.7 per cent), and decline thereafter. data are presented on how geographic location, species, and time of year affect surveillance results. the correlation of carnivore plague surveillance with human cases in discussed.19827125037
[use of cyclophosphamide-immunodepressed mice in the diagnosis of plague]. 19827186798
how accessible are physicians? some findings and implications of the changing distribution of general practitioners in three new zealand cities.an understanding of the changing distribution of general practitioners within urban areas is considered necessary as a first step towards understanding the problems of developing primary care services. both the distribution of general practitioners and the pattern of change 1966 to 1974 are analysed for three urban centres: auckland, wellington-hutt and christchurch. results indicate that newer suburbs are less well served than either established neighbourhoods or areas of high socio-economic st ...1982272508
[disorders in the position of teeth in patients with cleft palate]. 1982272279
[japanese literature on antibiotics. clxvi]. 1982351235
repression of the virulence of yersinia pestis by an f' plasmid.an f-lac plasmid from escherichia coli was transferred to virulent yersinia pestis, resulting in the repression of virulence. the y. pestis f-lac clones retained all of the known virulence traits but were avirulent and calcium independent. every lac segregant derived from the f-lac clones was fully virulent and calcium dependent.19836832826
entomological, bacteriological and serological observations after the 1977 plague outbreak in mbulu district, tanzania. 19836617551
[restoration of natural plague foci resulting from endemics of the causative agent of the disease in various animals]. 19836888331
chronic infections in laboratory rodents from inoculation of nonencapsulated plague bacilli (yersinia pestis).infections with nonencapsulated y. pestis persisted for 9 weeks in mice and 56 weeks in rats after experimental inoculation. mice succumbed with bacteremia. rats developed chronic lesions containing nonencapsulated plague bacilli.19836832323
vwa+ phenotype of yersinia enterocolitica.expression of the vwa+ phenotype of yersinia pestis in vitro is known to reflect maximum induction of virulence (or v and w antigens) at 37 degrees c with concomitant restriction of cell division. both phenomena are potentiated by 20 mm mg2+ and prevented by cultivation at 26 or 37 degrees c with 2.5 mm ca2+. we have now compared this classic plasmid-mediated phenotype with those of vwa+ yersinia pseudotuberculosis and yersinia enterocolitica which, unlike y. pestis, produce ancillary outer memb ...19836832830
serologic testing of badgers to monitor plague in southwestern idaho.serologic testing of badgers (taxidea taxus) was used to monitor plague (yersinia pestis) in a townsend ground squirrel (spermophilus townsendi) population in 10,000 ha of the snake river birds of prey study area, idaho. eighty-six percent of the 294 sera tested in 1975 and in 1976 were positive. significantly fewer (72%) seropositives occurred in 1977. seasonal changes in the percentage of seropositives and the decline in 1977 were probably due to the phenology of the townsend ground squirrel a ...19836842729
[effect of prolonged stay in the organism of the tick ornithodoros tartakovskyi (argasidae) on the properties of yersinia pestis]. 19836844033
[effect of the repeated infecting feeding with plague microbe on block formation in fleas (siphonaptera)]. 19836844034
[discrete nature of the antibody affinity of immune sera and the importance of this factor for antigen binding].after mixing an antibody immunosorbent with pasteurella pestis monovalent capsular antigen the concentration of the antigen in the free and bound states was evaluated. in this study the test points on the graph were found to be located on the lines characterizing different groups of antibodies differing in their affinity. the verification of the discrete structure of antibody affinity in 10 lots of hyperimmune horse serum showed the truth of this suggestion. the analysis of the role played by di ...19836868893
leads from the mmwr. plague--south carolina. 19836876307
plague in south african rodents 1972-1981.sera from 3012 rodents of 24 species captured in south africa during the period 1972-81 were tested for antibody to the fraction 1 antigen of yersinia pestis by passive haemagglutination. antibodies were found in seven (0.23%) rodents of three species. these were desmodillus auricularis and tatera brantsii in the northern cape province and rhabdomys pumilio in the eastern cape province. rodents were found positive in 1972, 1974, 1975 and 1979, indicating that plague continues to circulate in rod ...19836868103
diagnosis complicated by group a streptococcus. 19836367461
[antigenic activity of external membrane proteins in yersinia pestis ev].the study of immunocomplexes obtained from antisera to the preparations of y. pestis outer membranes and membrane proteins has revealed that outer membrane proteins are involved in the formation of the immunocomplex and belong to previously unknown y. pestis ev antigens.19836405562
[precipitation of the antigens of the causative agents of plague, glanders and melioidosis using aqueous saline extracts of various plant species].the interaction of the aqueous saline extracts of 55 plant species with the antigens of the causative agents of plague, glanders and melioidosis in the reaction of immunodiffusion (rid) in gel has been studied. the aqueous saline extracts obtained from the seeds of 12 plant species have been revealed to possess precipitating capacity. at the same time these extract have been found to ensure, as a rule, rid with several antigens under test.19836419513
warning on a new potential for laboratory-acquired infections as a result of the new nomenclature for the plague bacillus.the new name for the plague bacillus (formerly "yersinia pestis") is y. pseudotuberculosis subsp. pestis, while the pseudotuberculosis bacillus is named y. pseudotuberculosis subsp. pseudotuberculosis. any tendency to abbreviate these names on labels of cultures, especially by omitting the subspecies epithet, must be avoided because of the risk of confusion between the dangerous plague organism and the pseudotuberculosis bacillus.19836603926
[localization of antigen f-1 of yersinia pestis ev by an immunoferritin method].the localization of antigen f-1 in y. pestis strain ev, line (formula; see text) has been studied by means of specific monoreceptor ferritin-labeled antibodies. the study has given direct proofs that antigen f-1 is localized on the surface of the cell wall and in bacterial microcapsules. the substance of the microcapsule is loosely bound with the cell body and easily diffuses into the intercellular space, the antigenic properties of the substance remaining unchanged.19836624313
[dna-methyltransferase of the plague microbe].dna-methyltransferases of yersinia pestis ev, plague agent bacteria were isolated by p-ii phosphocellulose chromatography. the methylating activity is eluated by two fractions at the 0.47 m and 0.53 m nacl concentrations. methylases of the plague microbe are specific with respect to two bases (adenine and cytosine) and are capable of modifying both native and denaturated form of dna.19836636308
[isolation and purification of yersinia pestis neuraminidase]. 19836637277
isolation and identification of ropy bacteria in raw milk.approximately 4.2% of 4,000 maryland-virginia raw milk tanker samples developed ropiness when incubated at 10 degrees c. of the 56 bacterial isolates 30 were identified by species. klebsiella oxytoca and pseudomonas aeruginosa were isolated most frequently. other ropy isolates were identified as pseudomonas spp., chromobacterium, flavobacterium multivorum, presumptive yersinia pestis, enterobacter agglomerans, klebsiella pneumoniae, and pasteurella-actinobacter spp. six of the klebsiella oxytoca ...19836685140
[molecular organization and functions of the protein toxins of the causative agents of cholera and plague]. 19836362301
[concentration and purification of d'erell's phage]. 19836086049
[construction of a suspension diagnosticum to test for yersinia pestis endotoxin and homologous antibodies]. 19836195409
constitutive uptake and degradation of fatty acids by yersinia pestis.yersinia pestis was found to utilize palmitic acid as a primary carbon and energy source. no inhibition of growth by palmitic acid was observed. comparison of palmitic acid uptake by cells pregrown either with or without palmitic acid demonstrated that fatty acid uptake was constitutive. high basal levels of two enzymes of beta-oxidation, beta-hydroxyacyl-coenzyme a dehydrogenase and thiolase, and the two enzymes of the glyoxylate shunt, isocitrate lyase and malate synthase, were found in cells ...19836129238
[attempt at the experimental infection of isabelline wheatears (oenanthe isabellina temm., 1829) with the causative agent of plague]. 19836225008
[translocation of transposons tn10 and tn5 in the yersinia pestis chromosome].the possibility of translocation of the transposons tn5 and tn10 into the genome of yersinia pestis, with the subsequent mutagenic effect was demonstrated. we revealed transposon harbouring clones at frequency 10(-4) to 10(-2). derivatives of p1cml clr100ts phage served as vectors. insertion of tn10 transposon induced mutations in ilv, ser, arg, pur, pro, leu, nic, tyr, gua genes. the number of the insertion sites on the chromosome obtained for tn5 was the same, these being arg, ade, pyr, leu, g ...19836317517
ecology of plague in africa: response of indigenous wild rodents to experimental plague infection.the mastomys natalensis species complex, subdivided into genetically distinct species having diploid chromosome numbers 2n = 32 and 2n = 36, is a reservoir for several zoonoses including lassa fever and plague. this report describes a study to determine whether these sibling species and three other rodent species have different potential as reservoirs for plague. it was found that m. natalensis (2n = 32) was significantly more resistant to experimental plague infection (50% survived inoculation ...19836345015
[analysis of the discrete structure of antibodies by affinity and antigen-binding capacity].immune sera are a mixture of different groups of antibodies belonging to the same class of immunoglobulins, but differing in their affinity. the graphic analysis and numerical methods permit the determination of the size and affinity of each group of antibodies. differences in affinity are manifested as differences in antigen-binding capacity. the antigen-binding capacity index, calculated as the product of the association constant multiplied by the concentration of the active centers of antibod ...19836362294
[epizootic process of natural focus infections from the viewpoint of systems theory]. 19836362295
[quantitative characteristics of the survival phenomenon in experimental plague].the method for the quantitative evaluation of the effect of the survival phenomenon is proposed, which makes it possible to carry out the rapid single-stage determination of the immunogenicity of pasteurella pestis avirulent strains. the low specificity of the survival phenomenon in plague has been shown. the accumulation of immunogenic avirulent cells in the population of virulent p. pestis has been found to decrease their pathogenic action to a greater extent than the accumulation of nonimmuno ...19836362297
[attempt at detecting the systems of host specificity in the bacterial causative agents of plague].an attempt at revealing the system of host specificity in y. pestis by the methods of transformation and conjugation has been made. no endonuclease restriction has been detected.19836362298
[methods of preparing and purifying the capsular antigen and endotoxin of the causative agent of plague]. 19836201660
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