Publications

molecular cloning, expression and characterization of pectin methylesterase (ctpme) from clostridium thermocellum.	many phytopathogenic micro-organisms such as bacteria and fungi produce pectin methylesterases (pme) during plant invasion. plants and insects also produce pme to degrade plant cell wall. in the present study, a thermostable pectin methylesterase (ctpme) from clostridium thermocellum belonging to family 8 carbohydrate esterase (ce8) was cloned, expressed and purified. the amino acid sequence of ctpme exhibited similarity with pectin methylesterase from erwinia chrysanthemi with 38% identity. the ...	2017	28332030
a cost-effective protocol for the over-expression and purification of fully-functional and more stable erwinia chrysanthemi ligand-gated ion channel.	the erwinia chrysanthemi ligand-gated ion channel, elic, is considered an excellent structural and functional surrogate for the whole pentameric ligand-gated ion channel family. despite its simplicity, elic is structurally capable of undergoing ligand-dependent activation and a concomitant desensitization process. to determine at the molecular level the structural changes underlying elic's function, it is desirable to produce large quantities of protein. this protein should be properly folded, f ...	2017	28279818
structural basis of alcohol inhibition of the pentameric ligand-gated ion channel elic.	the structural basis for alcohol modulation of neuronal pentameric ligand-gated ion channels (plgics) remains elusive. we determined an inhibitory mechanism of alcohol on the plgic erwinia chrysanthemi (elic) through direct binding to the pore. x-ray structures of elic co-crystallized with 2-bromoethanol, in both the absence and presence of agonist, reveal 2-bromoethanol binding in the pore near t237(6') and the extracellular domain (ecd) of each subunit at three different locations. binding to ...	2017	27916519
attenuation and quantitation of virulence gene expression in quorum-quenched dickeya chrysanthemi.	n-acyl-homoserine lactones (ahls)-dependent quorum sensing (qs) system(s) is recruited by the soft rot bacterium dickeya chrysanthemi for coordinating its social activities such as secretion of plant cell wall-degrading enzymes, while the main signal molecule and quantity dependence of virulence to qs in this bacterium have not been clarified. to do this end, the involvement of ahls in african violet leaves and potato tuber maceration; swarming motility; pectate lyase and polygalacturonase enzym ...	2017	27496158
differential roles of iron storage proteins in maintaining the iron homeostasis in mycobacterium tuberculosis.	ferritins and bacterioferritins are iron storage proteins that represent key players in iron homeostasis. several organisms possess both forms of ferritins, however, their relative physiological roles are less understood. mycobacterium tuberculosis possesses both ferritin (bfrb) and bacterioferritin (bfra), playing an essential role in its pathogenesis as reported by us earlier. this study provides insights into the role of these two proteins in iron homeostasis by employing m. tuberculosis bfr ...	2017	28060867