Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| curdlan, a (1----3)-beta-d-glucan from alcaligenes faecalis var. myxogenes ifo13140, activates the alternative complement pathway by heat treatment. | from the results of consumption experiments of guinea pig complement, curdlan, a (1----3)-beta-d-glucan obtained from alcaligenes faecalis var. myxogenes ifo13140, has been found to lack the ability to activate complement when unheated or preheated at either 40 degrees c or 50 degrees c. however, curdlan heated at or above 60 degrees c increased complement consumption. this activation, dependent on the temperature of the curdlan, was via the alternative complement pathway as assessed by cleavage ... | 1990 | 2276766 |
| [site-specific endonucleases lpli and aagi]. | new site-specific endonucleases lpli and aagi have been isolated from the lactobacillus plantarum and achromobacter agile cells, respectively. the enzymes' purification stages included treatment of cell-free extracts with polyethylenimine, fractionation in two-phase system by albertsson's method, chromatography on blue sepharose and deae-cellulose. the results of cleavage of a 5'-32p-labelled oligodeoxynucleotide duplex by restriction endonucleases lpli and aagi indicate that these enzymes recog ... | 1990 | 2285421 |
| [susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. reported by the research group for testing imipenem susceptibility on clinical isolates]. | this study was conducted to investigate susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antibacterial agents at 64 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were carried out at each laboratory and the tests were performed according to the disk dilution method recommended by nccls in which susceptibilities are classified into "s", "ms", "i" and "r". ipm showed markedly high in vit ... | 1990 | 2287060 |
| in vivo effect of the organophosphorus insecticide trichlorphon on immune response of carp (cyprinus carpio). ii. effect of high doses of trichlorphon on nonspecific immune response. | the effect of trichlorphon, one of the most widely used organophosphorus insecticides, on the nonspecific immune response in carp (cyprinus carpio) was studied. the effect of 20,000 ppm trichlorphon on the immune response was followed for 3 and 56 days after intoxication. the effect of 10,000 ppm trichlorphon on the nonspecific immune response of carp experimentally infected by pseudomonas alcaligenes and aeromonas punctata was also examined. leucocyte number, phagocytic ability of neutrophils, ... | 1990 | 2311567 |
| purification and characterization of dichloromuconate cycloisomerase from alcaligenes eutrophus jmp 134. | dichloromuconate cycloisomerase from alcaligenes eutrophus jmp 134 was purified to homogeneity. the enzyme has an mr of about 270,000 as determined by gel filtration and consists of six to eight subunits of identical mr 40,000 as determined by sds/page. mn2+ ions as well as thiol groups are required for activity. a high km value of about 4 mm for cis,cis-muconate explains the reported low activity with this compound. relatively high km values were also calculated for monochloro-substituted cis,c ... | 1990 | 2327971 |
| cyclic nature of poly(3-hydroxyalkanoate) metabolism in alcaligenes eutrophus. | the cyclic process of biosynthesis and degradation of poly(3-hydroxyalkanoate) (pha) was studied in alcaligenes eutrophus under conditions of nitrogen-limitation of growth. a. eutrophus cells, which had accumulated poly(3-hydroxybutyrate) (phb) of 55 wt% content within cells from butyric acid, were transferred into a nitrogen-free medium containing pentanoic acid as the sole carbon source and cultivated at 30 degrees c and ph 7.5. the content of phb in a. eutrophus cells decreased with time, whe ... | 1990 | 2328906 |
| catheter-related right-sided endocarditis in bone marrow transplant recipients. | bone marrow transplant recipients are at increased risk of severe central venous catheter-related septicemias that may be complicated by endocardial infection. in view of this, we prospectively evaluated 141 consecutive patients receiving allogeneic or autologous bone marrow infusion. seven (5%) of 141 patients developed eight episodes of a clinical syndrome compatible with catheter-related right-sided infective endocarditis; this diagnosis was confirmed at autopsy in two patients who died. stap ... | 1990 | 2330480 |
| trichloroethylene degradation by two independent aromatic-degrading pathways in alcaligenes eutrophus jmp134. | the bacterium alcaligenes eutrophus jmp134(pjp4) degrades trichloroethylene (tce) by a chromosomal phenol-dependent pathway and by the plasmid-encoded 2,4-dichlorophenoxyacetic acid pathway. the two pathways were independent and exhibited different rates of removal and capacities for quantity of tce removed. the phenol-dependent pathway was more rapid (0.2 versus 0.06 nmol of tce removed per min per mg of protein) and consumed all detectable tce. the 2,4-dichlorophenoxyacetic acid-dependent path ... | 1990 | 2339875 |
| degradation of the chlorinated phenoxyacetate herbicides 2,4-dichlorophenoxyacetic acid and 2,4,5-trichlorophenoxyacetic acid by pure and mixed bacterial cultures. | combined cell suspensions of the 2,4,5-trichlorophenoxyacetic acid (2,4,5-t)-metabolizing organism pseudomonas cepacia ac1100, and the 2,4-dichlorophenoxyacetic acid (2,4-d)-metabolizing organism alcaligenes eutrophus jmp134 were shown to effectively degrade either of these compounds provided as single substrates. these combined cell suspensions, however, poorly degraded mixtures of the two compounds provided at the same concentrations. growth and viability studies revealed that such mixtures of ... | 1990 | 2339889 |
| achromobacter group b replacement valve endocarditis. | 1990 | 2341737 | |
| [effects of indices of the test-system "age--normal antibodies--leukocytes--lymphocytes" on immunological parameters under the action of cancer risk factors]. | it is established that application of the test-system "age-normal antibodies-leucocytes-lymphocytes" permits refusing in certain cases from the generally-accepted laboratory immunological tests and prognosticate their values depending on the test-system indices when using the regression models. the great incongruity of the predicted and real values of the concrete immunological index may be the manifestation of the risk factors associated with the oncological and other diseases. | 1990 | 2344822 |
| a survey of the microflora of raw and pasteurized milk and the sources of contamination in a milk processing plant in addis ababa, ethiopia. | the microorganisms present in raw and pasteurized milk and the sources of contamination in the milk after it had arrived at the processing plant in addis ababa were studied. the lowest count registered for raw milk samples was 4 x 10(7) cfu/ml while the highest was 1 x 10(9) cfu/ml. pasteurized milk had mesophilic aerobic counts of 7 x 10(5) cfu/ml as it left the pasteurizing unit, but the population increased 2- to 4-fold as a result of subsequent contamination. of the total counts in raw milk, ... | 1990 | 2345191 |
| two resistance phenotypes to beta-lactams of alcaligenes denitrificans subsp. xylosoxydans in relation to beta-lactamase types. | 1990 | 2351630 | |
| nucleotide sequence of metapyrocatechase i (catechol 2,3-oxygenase i) gene mpci from alcaligenes eutrophus jmp222. | 1990 | 2356133 | |
| action of endo-alpha-n-acetylgalactosaminidase from alcaligenes sp. on amino acid-o-glycans: comparison with the enzyme from diplococcus pneumoniae. | endo-alpha-n-acetylgalactosaminidase from alcaligenes sp. released the disaccharide, gal beta 1----3galnac, from both dansylated serine-galnac-gal and threonine-galnac-gal, and showed higher activity on the former than the latter. the km values were 0.17 mm and 1.43 mm with dns-ser-galnac-gal and dns-thr-galnac-gal, respectively. the optimum phs were found to be 4.5-7.5 and 4.5-6.0 on dns-ser-galnac-gal and dns-thr-galnac-gal, respectively. on the contrary, the enzyme from diplococcus pneumoniae ... | 1990 | 2357231 |
| steady-state nitric oxide concentrations during denitrification. | three species of denitrifying bacteria, paracoccus denitrificans, pseudomonas stutzeri strain jm300, and achromobacter cycloclastes, were allowed to reduce nitrate or nitrite in anaerobic, closed vials while the equilibration of gases between aqueous and gas phases was facilitated by vigorous stirring. the gas phase was sampled and analyzed for no with use of a chemiluminescence detector calibrated against bottled no standards or against no produced by the nitrite-iodide reaction. [noaq] was inf ... | 1990 | 2365685 |
| numerical analysis of electrophoretic protein patterns of 'achromobacter' group b, e and f strains from human blood. | thirty-two clinical strains representing 'achromobacter' groups b, e and f were characterized by one-dimensional sds-page of cellular proteins. all the strains were isolated from blood samples from hospital patients in the united kingdom. the protein patterns, which contained 40 to 45 discrete bands, were highly reproducible and were used as the basis for a numerical analysis which included all the protein bands. the 32 'achromobacter' strains formed two clusters at the 77% s level. the first, p ... | 1990 | 2370235 |
| susceptibility of alcaligenes denitrificans subspecies xylosoxydans to beta-lactam antibiotics. | the susceptibility of 56 clinical isolates and two reference strains of alcaligenes denitrificans subsp. xylosoxydans to beta-lactam agents was tested and related to beta-lactamase activity of the strains. the mics of 12 beta-lactams determined by an agar dilution method showed that all the strains were sensitive to imipenem and moxalactam. forty-one cloxacillin-sensitive beta-lactamase producing strains were highly susceptible to azlocillin, piperacillin and ticarcillin, and less susceptible to ... | 1990 | 2387293 |
| microbial metabolism of quinoline and related compounds. iv. degradation of isoquinoline by alcaligenes faecalis pa and pseudomonas diminuta 7. | from sewage and soil isoquinoline-degrading organisms were enriched. two strains could be isolated which were able to utilize isoquinoline as sole carbon source. the bacteria were tentatively identified as alcaligenes faecalis and pseudomonas diminuta with respect to their morphological and physiological characters. when growing on isoquinoline both strains excrete a metabolite into the medium which was identified as 1-oxo-1,2-dihydroisoquinoline. alcaligenes faecalis was cultivated in continuou ... | 1990 | 2390217 |
| classification of the spoilage flora of fish, with special reference to shewanella putrefaciens. | one hundred and fifty-nine gram-negative strains isolated from refrigerated fish, taken from the baltic sea or swedish inland waters, together with 32 reference strains of shewanella, pseudomonas, aeromonas and alcaligenes, were phenotypically classified using 124 unit characters. data were processed by the simple matching (ssm) and jaccard (sj) coefficients, and unweighted pair group algorithm with arithmetic averages. fourteen clusters were defined at the 75% sj similarity level which correspo ... | 1990 | 2391295 |
| [effect of the isolation medium and isolation conditions on the isolation of the bacterial species spectrum in drinking water]. | with the method of colony-count estimation according to german standards (trinkwasserverordnung, tvo), bacteria of the genera aeromonas, shewanella, pseudomonas etc. and (if present) representatives of the family enterobacteriaceae can be detected. longer incubation and application of low nutrient media enhance the colony counts up to 100-fold, and other bacteria can be isolated, in most cases belonging to the genera pseudomonas, alcaligenes, acinetobacter, flavobacterium etc.. for the detection ... | 1990 | 2393490 |
| different types of dienelactone hydrolase in 4-fluorobenzoate-utilizing bacteria. | of various benzoate-utilizing bacteria tested, alcaligenes eutrophus 335, a. eutrophus h16, a. eutrophus jmp222, a. eutrophus jmp134, alcaligenes strain a7, and pseudomonas cepacia were able to grow with 4-fluorobenzoate as the sole source of carbon and energy. p. cepacia also utilizes 3-fluorobenzoate. except for a. eutrophus jmp134, which is known to grow with 2,4-dichlorophenoxyacetate and 3-chlorobenzoate (r. h. don and j. m. pemberton, j. bacteriol. 145:681-686, 1981), the strains were unab ... | 1990 | 2394679 |
| enzymatic formation, stability, and spontaneous reactions of 4-fluoromuconolactone, a metabolite of the bacterial degradation of 4-fluorobenzoate. | enzymatic conversion of 4-fluorocatechol in the simultaneous presence of partially purified preparations of catechol 1,2-dioxygenase from pseudomonas cepacia and muconate cycloisomerase from alcaligenes eutrophus 335 yielded a product that was unambiguously identified as (+)-4-fluoromuconolactone [(+)-4-carboxymethyl-4-fluoro-but-2-en-4-olide]. this compound was shown to be the only major product formed from 3-fluoro-cis,cis-muconate by the action of muconate cycloisomerases from a. eutrophus 33 ... | 1990 | 2394680 |
| the bacteriology of fresh and spoiling lake victorian nile perch (lates niloticus). | a total of 177 bacterial cultures isolated from lake victorian nile perch (lates niloticus) were investigated. the flora on newly caught nile perch consisted of organisms belonging to the genera moraxella, alcaligenes, acinetobacter, pseudomonas, aeromonas, micrococcus and other gram-positive organisms. 39% were identified as gram-positive species and 61% were negative in the gram-reaction. three cultures out of 53 investigated caused weak rotten off-odours in sterile fish broth and one culture, ... | 1990 | 2397157 |
| degradation of mono- and dichlorobenzoic acid isomers by two natural isolates of alcaligenes denitrificans. | 1990 | 2403263 | |
| induction and efficient purification of endo-alpha-n-acetylgalactosaminidase from alcaligenes sp. | 1990 | 1369985 | |
| dna probe-mediated detection of resistant bacteria from soils highly polluted by heavy metals. | alcaligenes eutrophus ch34 dna fragments encoding resistance to cd, co, zn (czc), or hg (mera) were cloned and used as probes in colony hybridization procedures with bacteria isolated from polluted environments such as a zinc factory area (desertified because of the toxic effects of zinc contamination) and from sediments from factories of nonferrous metallurgy in belgium and mine areas in zaire. from the different soil samples, strains could be isolated and hybridized with the czc probe (resista ... | 1990 | 16348196 |
| characterization of rhizobacteria associated with weed seedlings. | rhizobacteria were isolated from seedlings of seven economically important weeds and characterized for potential phytopathogenicity, effects on seedling growth, and antibiosis to assess the possibility of developing deleterious rhizobacteria as biological control agents. the abundance and composition of rhizobacteria varied among the different weed species. for example, fluorescent pseudomonads represented from 11 to 42% of the total rhizobacterial populations from jimsonweed and lambsquarters, ... | 1990 | 16348208 |
| bacterial degradation of ring-chlorinated acetophenones. | two strains, alcaligenes sp. strain aca and pseudomonas fluorescens acb, isolated from acetophenone and 4'-hydroxyacetophenone enrichments, respectively, cometabolize a range of chlorinated acetophenones (cas). a biological baeyer-villiger reaction converts the ca to chlorophenyl acetate. this is evident only in the presence of an esterase inhibitor, since the ca is normally rapidly hydrolyzed to a chlorophenol which has the same substitution pattern as the original ketone. the oxygenase that at ... | 1990 | 16348372 |
| microbial metabolism of tholin. | in this paper, we show that a wide variety of common soil bacteria are able to obtain their carbon and energy needs from tholin (a class of complex organic heteropolymers thought to be widely distributed through the solar system; in this case tholin was produced by passage of electrical discharge through a mixture of methane, ammonia, and water vapor). we have isolated aerobic, anaerobic, and facultatively anaerobic bacteria which are able to use tholin as a sole carbon source. organisms which ... | 1990 | 11538367 |
| activities, occurrence, and localization of hydrogenase in free-living and symbiotic frankia. | symbiotic and free-living frankia were investigated for correlation between hydrogenase activities (in vivo/in vitro assays) and for occurrence and localization of hydrogenase protein by western blots and immuno-gold localization, respectively. freshly prepared nodule homogenates from the symbiosis between alnus incana and a local source of frankia did not show any detectable in vivo or in vitro hydrogenase uptake activity, as also has been shown earlier. however, a free-living frankia strain or ... | 1990 | 16667353 |
| rapid identification of bordetella avium and related organisms on the basis of their cellular carbohydrate patterns. | whole cell hydrolysates of bordetella avium, b. bronchiseptica, b. pertussis, b. parapertussis, alcaligenes faecalis, a. xylosoxidans ssp. denitrificans, a. xylosoxidans ssp. xylosoxidans, and a. piechaudii were analysed for their cellular carbohydrates by capillary gas-chromatography of per-acetylated aldononitriles and o-methyloximes, respectively. all alcaligenaceae species could be discriminated on the basis of their carbohydrate profiles and a few conventional features. taxometric evaluatio ... | 1991 | 18680060 |
| purification and characterization of d-aminoacylase from alcaligenes faecalis da1. | a d-aminoacylase from alcaligenes faecalis da1 has been purified to homogeneity by a simple purification procedure with two columns, fractogel deae-650 and hw-50. the specific activity of the purified enzyme was found to be 580 u/mg of protein with n-acetyl-dl-methionine as the reaction substrate. the apparent molecular weight and isoelectric point of this enzyme were determined to be 55,000 and 5.4, respectively. | 1991 | 16348465 |
| transfer and expression of the catabolic plasmid pbrc60 in wild bacterial recipients in a freshwater ecosystem. | 3-chlorobenzoate (3cba)-degrading bacteria were isolated from the waters and sediments of flowthrough mesocosms dosed with various concentrations of 3cba and inoculated with a 3cba-degrading alcaligenes sp., strain br60. bacteria capable of 3cba degradation which were distinct from br60 were isolated. they carried pbrc60, a plasmid introduced with alcaligenes sp. strain br60 that carries a transposable element (tn5271) encoding 3cba degradation. the isolates expressed these genes in different wa ... | 1991 | 16348493 |
| determinants encoding resistance to several heavy metals in newly isolated copper-resistant bacteria. | three copper-resistant, gram-negative bacteria were isolated and characterized. of the three strains, alcaligenes denitrificans ah tolerated the highest copper concentration (mic = 4 mm cuso(4)). all three strains showed various levels of resistance to other metal ions. a. denitrificans ah contains sequences which cross-hybridized with the mer (mercury resistance) determinant of tn21 and the czc (cobalt, zinc, and cadmium resistance), cnr (cobalt and nickel resistance), and chr (chromate resista ... | 1991 | 16348575 |
| high-level nickel resistance in alcaligenes xylosoxydans 31a and alcaligenes eutrophus kto2. | two new nickel-resistant strains of alcaligenes species were selected from a large number (about 400) of strains isolated from ecosystems polluted by heavy metals and were studied on the physiological and molecular level. alcaligenes xylosoxydans 31a is a heterotrophic bacterium, and alcaligenes eutrophus kto2 is an autotrophic aerobic hydrogen-oxidizing bacterium. both strains carry-among other plasmids-a megaplasmid determining resistance to 20 to 50 mm nicl(2) and 20 mm cocl(2) (when growing ... | 1991 | 16348590 |
| detection of plasmid transfer from pseudomonas fluorescens to indigenous bacteria in soil by using bacteriophage phir2f for donor counterselection. | the transfer of a genetically marked derivative of plasmid rp4, rp4p, from pseudomonas fluorescens to members of the indigenous microflora of the wheat rhizosphere was studied by using a bacteriophage that specifically lyses the donor strain and a specific eukaryotic marker on the plasmid. transfer of rp4p to the wheat rhizosphere microflora was observed, and the number of transconjugants detected was approximately 10 transconjugants per g of soil when 10 donor cells per g of soil were added; tr ... | 1991 | 16348599 |
| an activated sludge process to reduce the pollution load of a dye-industry waste. | a laboratory-scale activated sludge process was developed to reduce the pollution load of a dye-industry waste, containing aniline, phenol, methyl violet and rhodamine b as its major components. the waste exerted an organic load of 5576 mg litre(-1) as the chemical oxygen demand (cod), of 896 mg litre(-1) as total organic carbon (toc), and had a 31.5 mg litre(-1) phenol content. a microbial sludge, capable of growing on the waste, was developed from cattle dung, adapted to the waste and used as ... | 1991 | 15092144 |
| phylogenetic analysis and evolution of rnase p rna in proteobacteria. | the secondary structures of the eubacterial rnase p rnas are being elucidated by a phylogenetic comparative approach. sequences of genes encoding rnase p rna from each of the recognized subgroups (alpha, beta, gamma, and delta) of the proteobacteria have now been determined. these sequences allow the refinement, to nearly the base pair level, of the phylogenetic model for rnase p rna secondary structure. evolutionary change among the rnase p rnas was found to occur primarily in four discrete str ... | 1991 | 1711030 |
| evidence for two sets of structural genes coding for ribulose bisphosphate carboxylase in thiobacillus ferrooxidans. | previously, we reported the cloning of the ribulose-1,5-bisphosphate carboxylase genes (rbcl1-rbcs1) of thiobacillus ferrooxidans fe1 (t. kusano, k. sugawara, c. inoue, and n. suzuki, curr. microbiol. 22:35-41, 1991). with these genes as probes, a second set of ribulose-1,5-bisphosphate carboxylase genes (rbcl2-rbcs2) was identified in the same strain and cloned. rbcl1 and rbcl2 encode the large subunits, and rbcs1 and rbcs2 encode the small subunits. similar restriction patterns between these g ... | 1991 | 1718945 |
| na+/h+ exchange modulates rat neutrophil mediated tumor cytotoxicity. | rat neutrophils stimulated with phorbol 12-myristate 13-acetate, bacillus calmette-guérin, zymosan a, and beta-1,3-d-glucan from alcaligenes faecalis showed cytotoxicity to various tumor cells. hydrogen peroxide was shown to be an effector molecule in tumor cytotoxicity by inhibition using various active oxygen scavengers. the following findings suggest that tumor cytotoxicity by rat neutrophils stimulated with the four reagents mentioned above is regulated by na+/h+ exchange: (a) an increase in ... | 1991 | 1645615 |
| spectral properties of achromobacter xylosoxidans cytochromes c' and their no complexes. | cytochromes c' have been isolated from six strains of achromobacter xylosoxidans: ncib 11015 (formerly alcaligenes sp. ncib 11015), gifu 543, 1048, 1051, 1055 and 1764. they are dimeric proteins with more positive redox potentials than those of cytochromes c' from phototrophic bacteria at neutral ph. the electronic absorption, epr and mcd spectra on no-ferrous cytochromes c' at physiological ph showed that the major part of the heme-iron of nitrosylheme was penta-coordinated. the epr spectral re ... | 1991 | 1646026 |
| proton and tritium nmr relaxation studies of peptide inhibitor binding to bacterial collagenase: conformation and dynamics. | the interaction of succinyl-pro-ala, a competitive inhibitor of achromobacter iophagus collagenase, with the enzyme was studied by longitudinal proton and tritium relaxation. specific deuterium and tritium labeling of the succinyl part at vicinal positions allowed the measurement of the cross-relaxation rates of individual proton or tritium spin pairs in the inhibitor-enzyme complex as well as in the free inhibitor. overall correlation times, internuclear distances, and qualitative information o ... | 1991 | 1651124 |
| identification and characterization of two alcaligenes eutrophus gene loci relevant to the poly(beta-hydroxybutyric acid)-leaky phenotype which exhibit homology to ptsh and ptsi of escherichia coli. | from genomic libraries of alcaligenes eutrophus h16 in lambda l47 and in pvk100, we cloned dna fragments which restored the wild-type phenotype to poly(beta-hydroxybutyric acid) (phb)-leaky mutants derived from strains h16 and jmp222. the nucleotide sequence analysis of a 4.5-kb region of one of these fragments revealed two adjacent open reading frames (orf) which are relevant for the expression of the phb-leaky phenotype. the 1,799-bp orf1 represented a gene which was referred to as phbi. the a ... | 1991 | 1653223 |
| chlorobenzoate catabolic transposon tn5271 is a composite class i element with flanking class ii insertion sequences. | the structure of a transposon specifying the biodegradation of chlorobenzoate contaminants is described. tn5271 is a 17-kilobase (kb) transposon that resides in the plasmid or chromosome of alcaligenes sp. strain br60 and allows this organism to grow on 3- and 4-chlorobenzoate. the transposon is flanked by a directly repeated sequence of 3201 base pairs (bp), which in turn is flanked by 110-bp inverted repeats. the 3.2-kb repeated sequence, designated is1071, exists in multiple copies in the gen ... | 1991 | 1656436 |
| conjugational transfer of recombinant dna in cultures and in soils: host range of pseudomonas putida tol plasmids. | recombinant tol plasmid pwwo-eb62 allows pseudomonas putida to grow on p-ethylbenzoate. this plasmid can be transferred to other microorganisms, and its catabolic functions for the metabolism of alkylbenzoates are expressed in a limited number of gram-negative bacteria, including members of pseudomonad rrna group i and escherichia coli. transfer of the recombinant plasmid to erwinia chrysanthemi was observed, but transconjugants failed to grow on alkylbenzoates because they lost catabolic functi ... | 1991 | 1660698 |
| production of r-(-)-mandelic acid from mandelonitrile by alcaligenes faecalis atcc 8750. | r-(-)-mandelic acid was produced from racemic mandelonitrile by alcaligenes faecalis atcc 8750. ammonium acetate or l-glutamic acid as the carbon source and n-butyronitrile as the inducer in the culture medium were effective for bacterial growth and the induction of r-(-)-mandelic acid-producing activity. the r-(-)-mandelic acid formed from mandelonitrile by resting cells was present in a 100% enantiomeric excess. a. faecalis atcc 8750 has an r-enantioselective nitrilase for mandelonitrile and a ... | 1991 | 1660699 |
| [structure and function of cytochrome c' from achromobacter xylosoxidans]. | 1991 | 1665239 | |
| achromobacter xylosoxidans infection in baboons. | 1991 | 1666159 | |
| puncture wound-induced achromobacter xylosoxidans osteomyelitis of the foot. | 1991 | 1674638 | |
| biodegradation of 4-chlorophenol by adsorptive immobilized alcaligenes sp. a 7-2 in soil. | alcaligenes sp. a 7-2 immobilized on granular clay has been applied in a percolator to degrade 4-chlorophenol in sandy soil. good adsorption rates on granular clay were achieved using cell suspensions with high titres and media at ph 8.0. the influence of various parameters such as aeration rate, ph, temperature, concentration of 4-chlorophenol and size of inoculum on the degradation rate were investigated. during fed-batch fermentations under optimal culture conditions, concentrations of 4-chlo ... | 1991 | 1367580 |
| liquid fluidized bed adsorption of protein in the presence of cells. | the adsorption, in a liquid fluidized bed, of bovine serum albumin (bsa), onto an ion-exchange absorbent, q-sepharose fast flow, in the presence of alcaligenes eutrophus cells, has been studied. the expansion of the fluidized bed is greater in the presence than in the absence of cells and obeys the laws of richardson and zaki. the effect of cell concentration on the equilibrium adsorption characteristics of the adsorbent has been assessed. the rate of adsorption of bsa onto the adsorbent has bee ... | 1991 | 1368077 |
| combined chemical and mechanical processes for the disruption of bacteria. | mechanical cell disruption by high pressure homogenisation or high speed bead mills is currently the general method of choice for the large scale disruption of micro-organisms; however, deleterious effects include the high energy requirement, the need for efficient cooling to prevent the excessive heating of the product and the micronisation of cell debris. certain chemical treatments for microbial cell disruption alter the permeability of bacteria and yeasts, allowing partial release of soluble ... | 1991 | 1368079 |
| the disruption of alcaligenes eutrophus by high pressure homogenisation: key factors involved in the process. | the disruption of the gram-negative bacterium alcaligenes eutrophus by high pressure homogenisation, using the apv gaulin 15m 8ba and 30cd homogenisers is reported. the operating parameters such as operating pressure, number of passes, temperature and biomass concentration, mimicked trends previously reported for yeasts. extension of the study to consider the effect of cell characteristics, including the growth rate, size and shape, illustrated the dominant effect of the growth phase. the improv ... | 1991 | 1368082 |
| mineralization of 2,4-dichlorophenoxyacetic acid (2,4-d) in soil inoculated with pseudomonas cepacia dbo1(pro101), alcaligenes eutrophus aeo106(pro101) and alcaligenes eutrophus jmp134(pjp4): effects of inoculation level and substrate concentration. | mineralization of 2,4-dichlorophenoxyacetic acid (2,4-d) by two alcaligenes eutrophus strains and one pseudomonas cepacia strain containing the 2,4-d degrading plasmids pjp4 or pro101 (= pjp4::tn1721) was tested in 50 g (wet wt) samples of non-sterile soil. mineralization was measured as 14c-co2 evolved during degradation of uniformly-ring-labelled 14c-2,4-d. when the strains were inoculated to a level of approximately 10(8) cfu/g soil, between 20 and 45% of the added 2,4-d (0.05 ppm, 10 ppm or ... | 1991 | 1282056 |
| molecular analysis of the alcaligenes eutrophus poly(3-hydroxybutyrate) biosynthetic operon: identification of the n terminus of poly(3-hydroxybutyrate) synthase and identification of the promoter. | molecular methods have been applied to analyze the expression of the alcaligenes eutrophus poly(3-hydroxybutyrate) (phb) synthase gene (phbc). the translational initiation codon was identified by analysis of the amino acid sequence of a phb synthase-beta-galactosidase fusion protein. this protein was purified to almost gel electrophoretic homogeneity by chromatography on deae-sephacel and on aminophenyl-beta-d-thiogalactopyranoside-sepharose from cells of a. eutrophus which harbored a phbc'-'lac ... | 1991 | 1987116 |
| cloning and characterization of plasmid-encoded genes for the degradation of 1,2-dichloro-, 1,4-dichloro-, and 1,2,4-trichlorobenzene of pseudomonas sp. strain p51. | pseudomonas sp. strain p51 is able to use 1,2-dichlorobenzene, 1,4-dichlorobenzene, and 1,2,4-trichlorobenzene as sole carbon and energy sources. two gene clusters involved in the degradation of these compounds were identified on a catabolic plasmid, pp51, with a size of 110 kb by using hybridization. they were further characterized by cloning in escherichia coli, pseudomonas putida kt2442, and alcaligenes eutrophus jmp222. expression studies in these organisms showed that the upper-pathway gene ... | 1991 | 1987135 |
| metabolism of poly(3-hydroxyalkanoates) (phas) by pseudomonas oleovorans. identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of pha. | pseudomonas oleovorans accumulates poly(3-hydroxyalkanoates) (phas) after growth on medium chain length hydrocarbons. large amounts of this polyester are synthesized when cells are grown under nitrogen-limiting conditions. when nitrogen is resupplied in the medium, the accumulated pha is degraded. in this paper, we describe mutants which are defective in the synthesis or in the degradation of pha. these mutants were used to select dna fragments which encode pha polymerases and a pha depolymerase ... | 1991 | 1989978 |
| p-cresol methylhydroxylase from a denitrifying bacterium involved in anaerobic degradation of p-cresol. | a bacterium, strain pc-07, previously isolated as part of a coculture capable of growing on p-cresol under anaerobic conditions with nitrate as the acceptor was identified as an achromobacter sp. the first enzyme of the pathway, p-cresol methylhydroxylase, which converts its substrate into p-hydroxybenzyl alcohol, was purified. the enzyme had an mr of 130,000 and the spectrum of a flavocytochrome. it was composed of flavoprotein subunits of mr 54,000 and cytochrome subunits of mr 12,500. the mid ... | 1991 | 1991722 |
| three trans-acting regulatory functions control hydrogenase synthesis in alcaligenes eutrophus. | random tn5 mutagenesis of the regulatory region of megaplasmid phg1 of alcaligenes eutrophus led to the identification of three distinct loci designated hoxa, hoxd, and hoxe. sequencing of the hoxa locus revealed an open reading frame which could code for a polypeptide of 482 amino acids with a molecular mass of 53.5 kda. a protein of comparable apparent molecular mass was detected in heterologous expression studies with a plasmid-borne copy of the hoxa gene. amino acid alignments revealed strik ... | 1991 | 2001989 |
| sequence of the plasmid-encoded catechol 1,2-dioxygenase-expressing gene, pheb, of phenol-degrading pseudomonas sp. strain est1001. | phenol monooxygenase (pmo) and catechol 1,2-dioxygenase (c12o), the two first enzymes of the phenol-degradation pathways, are encoded by a 3.4-kb dna fragment cloned from pseudomonas sp. est1001 plasmid dna. we have previously shown that activation of the cloned genes in pseudomonas putida paw85 is controlled by insertion of the 17-kb transposon, tn4652, from the host chromosome into the plasmid carrying these genes [kivisaar et al. plasmid 24 (1990) 25-36]. transcription of the dna encoding pmo ... | 1991 | 2013408 |
| sequence analysis of the pseudomonas sp. strain p51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2-dioxygenases for chlorinated substrates. | pseudomonas sp. strain p51 contains two gene clusters located on catabolic plasmid pp51 that encode the degradation of chlorinated benzenes. the nucleotide sequence of a 5,499-bp region containing the chlorocatechol-oxidative gene cluster tcbcdef was determined. the sequence contained five large open reading frames, which were all colinear. the functionality of these open reading frames was studied with various escherichia coli expression systems and by analysis of enzyme activities. the first g ... | 1991 | 2013566 |
| human recombinant cuzn-superoxide dismutase. amino acid sequence and location of the disulfide bond. | the complete amino acid sequence of recombinant human cu-zn superoxide dismutase (cuznsod) is presented. the s-carboxymethylated protein was cleaved at lysine residues (with achromobacter protease i) to provide a set of nine non-overlapping fragments accounting for 90% of the sequence. these fragments were then overlapped and aligned, and the sequence was completed by using peptides generated by cleavage at glutamic acid residues (with s. aureus v8 protease) and at arginine (with clostripain). t ... | 1991 | 2019474 |
| catalysis of nitrosyl transfer by denitrifying bacteria is facilitated by nitric oxide. | two denitrifying bacteria, pseudomonas stutzeri and achromobacter cycloclastes, were incubated with na15no2 and nan3 under conditions that allowed catalysis of nitrosyl transfer from nitrite to azide. this transfer, which is presumed to be mediated by the heme- and copper-containing nitrite reductase of p. stutzeri and a. cycloclastes, respectively, leads to formation of isotopically mixed 14,15n2o, whereas denitrification leads to 15n2o. the conditions that emphasized nitrosyl transfer also par ... | 1991 | 2025262 |
| cdna-derived amino acid sequence of the nadh-binding 51-kda subunit of the bovine respiratory nadh dehydrogenase reveals striking similarities to a bacterial nad(+)-reducing hydrogenase. | a lambda gt10 bovine brain and a lambda gt11 bovine heart cdna library were screened with oligonucleotide probes corresponding to partial protein sequences directly determined from the isolated 51-kda subunit of the bovine respiratory-chain nadh dehydrogenase. clones were isolated that encode a protein of 464 amino acids containing all the 11 partial tryptic peptide sequences determined from the 51-kda subunit. the size and amino acid composition of this protein agree with those determined for t ... | 1991 | 2034666 |
| construction and properties of a triprotein containing the high-affinity nickel transporter of alcaligenes eutrophus. | the high-affinity nickel transporter of alcaligenes eutrophus h16 is encoded by gene hoxn, which maps within the hydrogenase gene cluster of megaplasmid phg1. a tripartite gene fusion was constructed, consisting of (i) the escherichia coli lacz gene for beta-galactosidase, (ii) a segment encoding an endoproteolytically cleavable peptide, and (iii) the a. eutrophus gene hoxn. an e. coli strain harboring this construct (plasmid pch307) efficiently produced the corresponding triprotein upon inducti ... | 1991 | 2037063 |
| [resistance of 700 gram-negative bacterial strains to antiseptics and antibiotics]. | the sensitivity of 701 gram negative strains, representing 16 species or bacterial genera, towards 4 antiseptics (cetrimide, chlorhexidine, hexachlorophene, mercuric chloride) and 6 antibiotics (ampicillin, streptomycin, erythromycin, chloramphenicol, kanamycin, tetracycline) was analysed. for 3 antiseptics (cetrimide, chlorhexidine, mercuric chloride) minimal inhibitory concentration distribution showed strains to be heterogeneous, particularly in the group pseudomonas. most strains were resist ... | 1991 | 2042903 |
| physiology and molecular genetics of poly(beta-hydroxy-alkanoic acid) synthesis in alcaligenes eutrophus. | the alcaligenes eutrophus genes for beta-ketothiolase, nadph-dependent acetoacetyl-coa reductase and poly(beta-hydroxybutyric acid) synthase (phb synthase) which comprise the three-step phb-biosynthetic pathway, were cloned. molecular studies revealed that these genes are organized in a single operon. the a. eutrophus phb-biosynthetic genes are readily expressed in other bacteria, and dna fragments harbouring the operon can be used as a cartridge to confer to other bacteria the ability to synthe ... | 1991 | 2046547 |
| detection and identification of substituted phenols as intermediates of concurrent bacterial degradation of the phenoxy herbicides mcpp and 2,4-d. | the concurrent bacterial degradation of 2-(2-methyl-4-chlorophenoxy)propionic acid and 2,4-dichlorophenoxyacetic acid was studied using a stirred tank reactor and a bacterial culture which had been originally derived by enrichment with mcpp. high pressure liquid chromatographic methodology was used to measure both herbicides and it also resolved the corresponding phenols as intermediates, i.e., 2-methyl-4-chlorophenol and 2,4-dichlorophenol. gas chromatography-mass spectrometry was used to verif ... | 1991 | 2060758 |
| identification and molecular characterization of the alcaligenes eutrophus h16 aco operon genes involved in acetoin catabolism. | acetoin:dichlorophenolindophenol oxidoreductase (ao:dcpip or) and the fast-migrating protein (fmp) were purified to homogeneity from crude extracts of acetoin-grown cells of alcaligenes eutrophus. ao:dcpip or consisted of alpha and beta subunits (mrs, 35,500 and 36,000, respectively), and a tetrameric alpha 2 beta 2 structure was most likely for the native protein. the molecular weight of fmp subunits was 39,000. the n-terminal amino acid sequences of the three proteins were determined, and olig ... | 1991 | 2061286 |
| molecular cloning and nucleotide sequence of the glycogen branching enzyme gene (glgb) from bacillus stearothermophilus and expression in escherichia coli and bacillus subtilis. | the structural gene for the bacillus stearothermophilus glycogen branching enzyme (glgb) was cloned in escherichia coli. nucleotide sequence analysis revealed a 1917 nucleotide open reading frame (orf) encoding a protein with an mr of 74787 showing extensive similarity to other bacterial branching enzymes, but with a shorter n-terminal region. a second orf of 951 nucleotides encoding a 36971 da protein started upstream of the glgb gene. the n-terminus of the orf2 gene product had similarity to t ... | 1991 | 1745226 |
| capillary gas chromatography of cellular carbohydrates as a means for the differentiation of fastidious, slow-growing or anaerobic gram-negative bacteria--a review. | the phenotypic differentiation of microaerophilic or anaerobic gram-negative bacteria of human origin is traditionally based on the analysis of metabolic characteristics. due to outstanding growth requirements of many kinds of parasitic bacteria, e.g. of the facultatively and strictly anaerobic members of the human periodontal flora, conventional biochemical tests are time-consuming and sometimes lead to doubtful results. commercial diagnostic test kits often produce misidentifications because o ... | 1991 | 1755919 |
| detection and identification of groundwater bacteria capable of escaping entrapment on 0.45-micron-pore-size membrane filters. | rural drinking water systems supplied by untreated groundwater were examined to determine whether coliform or heterotrophic plate count bacteria are capable of escaping entrapment on standard porosity (0.45-micron-pore-size) membrane filters. filterable bacteria were present in 42% of the 24 groundwater sources examined by using nonselective media (r2a, full strength m-hpc, and 0.1x m-hpc agars). pseudomonads were the most frequently identified group of filterable bacteria detected. flavobacteri ... | 1991 | 1768096 |
| identification of clinical isolates of gram-negative nonfermentative bacteria by an automated cellular fatty acid identification system. | an automated cellular fatty acid (cfa) bacterial identification system, microbial identification system (mis; microbial id, newark, del.), was compared with a conventional system for the identification of 573 strains of gram-negative nonfermentative bacteria. mis identifications were based exclusively on the cfa composition following 22 to 26 h of growth at 28 degrees c on trypticase soy agar. mis identifications were listed with a confidence measurement (similarity index [si]) on a scale of 0 t ... | 1991 | 1774302 |
| a zinc-binding protein in a metal-resistant strain, alcaligenes eutrophus ch34. | synthesis of a zinc-binding protein was induced when alcaligenes eutrophus ch34 was grown in the presence of high concentrations of zinc (2.3 mm). the zinc-binding protein has a low content of cysteine and a high content of acidic amino acids and, thus, differs from metallothionein. | 1991 | 1777862 |
| occurrence of poly-d(-)-3-hydroxyalkanoates in the genus bacillus. | a range of bacillus strains were examined for their ability to accumulate poly-d(-)-3-hydroxyalkanoates (poly-haks) which are naturally occurring materials that are optically active, biodegradable thermoplastics. the organisms could produce poly-d(-)-3-hydroxybutyrate (poly-hb) up to 50% of cell dry weight. the content of poly-hb in the cells varied with the growth conditions. the addition of propionate or valerate in the culture resulted in a synthesis of poly-d(-)-3-hydroxyvalerate (poly-hv). ... | 1991 | 1778440 |
| antitumor activity of polymorphonuclear leukocytes activated by a beta-1,3-d-glucan. | the antitumor activity of mouse polymorphonuclear leukocyte (pmn) treated with a beta-1,3-d-glucan from alcaligenes faecalis var. myxogenes ifo 13140 (tak-n) and its carboxymethylated derivative (cm-tak) was investigated in vitro and in vivo. icr mouse pmn showed strong cytotoxicity against sarcoma 180 cells and inhibition of the growth of the tumor cells in vitro in the presence of tak-n but not in the presence of cm-tak. since the cytotoxicity induced by tak-n was almost completely inhibited b ... | 1991 | 1779406 |
| identification of cfxr, an activator gene of autotrophic co2 fixation in alcaligenes eutrophus. | a regulatory gene, cfxr, involved in the carbon dioxide assimilation of alcaligenes eutrophus h16 has been characterized through the analysis of mutants. the function of cfxr is required for the expression of two cfx operons that comprise structural genes encoding calvin cycle enzymes. cfxr (34.8 kda) corresponds with an open reading frame of 954 bp, with a translational initiation codon 167 bp upstream of the chromosomal cfx operon. the cfx operon and cfxr are transcribed divergently. the n-ter ... | 1991 | 1779759 |
| purification and some properties of phospholipase c from achromobacter xylosoxidans. | a non-haemolytic phospholipase c (ec 3.1.4.3) was purified from the culture medium of achromobacter xylosoxidans with a 5% yield and a purification factor of 330. a combination of ultrafiltration, acetone precipitation and two subsequent affinity chromatographic steps was used. the affinity chromatography is a new application of 2-(4-aminophenylsulphonyl)ethyl-cellulose, a sorbent that has previously been used for the purification of phospholipase c from bacillus cereus. the purified enzyme gave ... | 1991 | 1783637 |
| identification of a locus within the hydrogenase gene cluster involved in intracellular nickel metabolism in bradyrhizobium japonicum. | a 0.6-kb fragment of dna involved in intracellular ni metabolism was isolated and cloned from a cosmid containing 23.2 kb of hydrogenase-related genes of bradyrhizobium japonicum. this locus is located 8.3 kb upstream of the hydrogenase structural genes. the hydrogenase activity of a mutant with a gene-directed mutation at this locus (strain jhk7) showed dependency on nickel provided during hydrogenase derepression. the hydrogenase activity was only 20% of that in the wild-type strain, jh, at a ... | 1991 | 1785925 |
| [the autoselection of neustonic forms of bacteria]. | self-breeding of neuston forms of methylobacterium sp., pseudomonas putida bc-2, alcaligenes paradoxus bc-1, bacillus thuringiensis var. israilensis bacteria as well as of a mixed culture of methylotrophs is shown possible. in spite of ability of hydrophobicity of the cell surface the suggested method of self-breeding may be used to perfect properties of larvicidal biopreparations, and bacterial preparations which intensify self-purification of water bodies. | 1991 | 1791780 |
| production of poly-d(-)-3-hydroxybutyrate and poly-d(-)-3-hydroxyvalerate by strains of alcaligenes latus. | alcaligenes latus strains can accumulate poly-d(-)-3-hydroxybutyrate (phb) up to about 85% of cell dry weight. the abilities to store poly-d(-)-3-hydroxyvalerate (phv) of three strains of a. latus were investigated. with na-propionate as phv precursor, strain a. latusdsm 1122 had better phv accumulation ability than strains a. latus dsm 1123 and 1124. strain a. latus dsm 1123 could store phv when na-valerate but not na-propionate served as the phv precursor. phb and phv accumulation by a. latus ... | 1991 | 1796809 |
| activity of meropenem against antibiotic-resistant or infrequently encountered gram-negative bacilli. | meropenem was compared in vitro with imipenem as well as with several other contemporary beta-lactams, ciprofloxacin, and gentamicin against a group of highly antibiotic resistant members of the family enterobacteriaceae and a collection of oxidase-positive and/or glucose-nonfermentative gram-negative bacilli. in this study, meropenem was more active than imipenem against isolates of enterobacter, klebsiella, morganella, providencia, alcaligenes, aeromonas, and pasteurella. | 1991 | 1804016 |
| qsars and pars for biodegradation of pcbs. | relationships between the biodegradation rate constants of a number of polychlorinated biphenyls (pcbs) and hydrophobic and electronic structural parameters are compared. there is no simple relationship with octanol-water partition coefficients, indicating that the biodegradation rates of pcbs are probably not determined by their rates of permeation through the bacterial membranes. biodegradation rate constants correlated much better with both the electronic and hydrophobic properties of the chl ... | 1991 | 1815355 |
| beta-d-glucuronidase (bdg) activity of gram-negative bacteria. | bdg is an inducible enzyme that is encoded by the uida gene in escherichia coli. genetic sequences of this gene are present in most if not all e. coli strains regardless of the bdg phenotype. expression of bdg activity can be influenced by lactose-induced catabolite repression or genetic mutations. salmonella, shigella and yersinia strains frequently exhibit positive bdg reaction. bdg activity of strains belonging to genus edwardsiella, serratia, yersinia, vibrio, erwinia, alcaligenes, acinetoba ... | 1991 | 1817425 |
| [achromobacter xylosoxidans septicemia]. | 1991 | 1827195 | |
| [an uncommon germ of meningitis, achromobacter xylosoxidans]. | 1991 | 1829828 | |
| amino acid sequence of nitrite reductase: a copper protein from achromobacter cycloclastes. | the amino acid sequence of the copper-containing nitrite reductase (ec 1.7.99.3) from achromobacter cycloclastes strain iam 1013 has been determined by using peptides derived from digestion with achromobacter protease i (lys), staphylococcus aureus v8 protease (glu), cyanogen bromide, and bnps-skatole in acetic acid. the subunit contains 340 amino acids. the identity of the first seven amino acids is tentative. the sequence has been instrumental in the x-ray structure determination of this molec ... | 1991 | 1830217 |
| primary structures of two subunits of nadh: ubiquinone reductase from neurospora crassa concerned with nadh-oxidation. relationship to a soluble nad-reducing hydrogenase of alcaligenes eutrophus. | the primary structures of the nuclear-encoded 51 kda and 78 kda subunits of the respiratory chain nadh: ubiquinone reductase (complex i) from neurospora crassa mitochondria were determined by sequencing cdna and the n-terminus of the mature proteins. both subunits are related to the soluble nad-reducing hydrogenase of the bacterium alcaligenes eutrophus. sequence comparison between these subunits, the corresponding subunits of the bovine complex i and the bacterial nad-reducing hydrogenase furth ... | 1991 | 1832016 |
| evolution of the rubisco operon from prokaryotes to algae: structure and analysis of the rbcs gene of the brown alga pylaiella littoralis. | the rbcs gene coding for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) of the brown alga pylaiella littoralis is located within the plastid genome and is transcribed as a single polycistronic mrna with the gene for the large subunit of rubisco, rbcl. the structure of the rubisco operon from p. littoralis was determined. molecular phylogenies for rbcs and rbcl with a wide range of prokaryotes and eukaryotes were constructed which are congruent with recent evidence ... | 1991 | 1840691 |
| natural transfer of conjugative transposon tn916 between gram-positive and gram-negative bacteria. | the conjugative streptococcal transposon tn916 was found to transfer naturally between a variety of gram-positive and gram-negative eubacteria. enterococcus faecalis hosting the transposon could serve as a donor for alcaligenes eutrophus, citrobacter freundii, and escherichia coli at frequencies of 10(-6) to 10(-8). no transfer was observed with several phototrophic species. mating of an e. coli strain carrying tn916 yielded transconjugants with bacillus subtilis, clostridium acetobutylicum, ent ... | 1991 | 1846142 |
| cloning, nucleotide sequence, and heterologous expression of a high-affinity nickel transport gene from alcaligenes eutrophus. | high-affinity nickel transport in alcaligenes eutrophus h16 is mediated by a function designated hoxn. hoxn lies within the hydrogenase gene cluster of megaplasmid phg1. an insertional mutation at the hoxn locus led to an increased nickel requirement. in this mutant (strain hf260) both autotrophic growth on hydrogen and wild-type level of urease, a nickel-containing enzyme, were dependent on high concentration of nickel in the medium. studies with a heterologous in vivo expression system reveale ... | 1991 | 1847142 |
| susceptibility of xanthomonas maltophilia and amikacin-resistant gram-negative bacteria to newer antimicrobials. | the susceptibility of xanthomonas maltophilia and amikacin-resistant gram-negative bacteria to a variety of newer antimicrobials was determined. sixty-three amikacin-resistant isolates were collected from 61 inpatients at a tertiary-care hospital between 1985 and 1988. the organisms included xanthomonas maltophilia (24 isolates), pseudomonas aeruginosa (16), pseudomonas species (3), serratia marcescens (10), flavobacterium meningosepticum (4), enterobacter species (3), acinetobacter calcoaceticu ... | 1991 | 1860303 |
| [the dna nucleotide composition of bacteria oxidizing diethylene glycols]. | nucleotide composition of dna has been analyzed in bacteria composing the diethylene glycol-oxidizing association. it is shown that the microorganisms under study are representatives of genera alkaligenes and achromobacter. | 1991 | 1861652 |
| the 2.3 angstrom x-ray structure of nitrite reductase from achromobacter cycloclastes. | the three-dimensional crystal structure of the copper-containing nitrite reductase (nir) from achromobacter cycloclastes has been determined to 2.3 angstrom (a) resolution by isomorphous replacement. the monomer has two greek key beta-barrel domains similar to that of plastocyanin and contains two copper sites. the enzyme is a trimer both in the crystal and in solution. the two copper atoms in the monomer comprise one type i copper site (cu-i; two his, one cys, and one met ligands) and one putat ... | 1991 | 1862344 |
| achromobacter xylosoxidans. an unusual neonatal pathogen. | perinatal acquisition of a rare pediatric pathogen, achromobacter xylosoxidans, with evidence for in utero transmission, is described. cultures from the mother and neonate demonstrated a. xylosoxidans. an ascending bacterial infection in the mother with clinical chorioamnionitis is presented as the probable mode of transmission. postmortem examination of the infant confirmed achromobacter meningitis. in contrast to the current case with transmission from mother to neonate, previously published n ... | 1991 | 1862776 |
| [sepsis caused by achromobacter xylosoxidans]. | 1991 | 1863629 | |
| novel cyanide-hydrolyzing enzyme from alcaligenes xylosoxidans subsp. denitrificans. | a cyanide-metabolizing bacterium, strain df3, isolated from soil was identified as alcaligenes xylosoxidans subsp. denitrificans. whole cells and cell extracts of strain df3 catalyzed hydrolysis of cyanide to formate and ammonia (hcn + 2h2o----hcooh + nh3) without forming formamide as a free intermediate. the cyanide-hydrolyzing activity was inducibly produced in cells during growth in cyanide-containing media. cyanate (ocn-) and a wide range of aliphatic and aromatic nitriles were not hydrolyze ... | 1991 | 1872607 |
| marine biosurfactants, i. screening for biosurfactants among crude oil degrading marine microorganisms from the north sea. | three bacterial strains of marine origin were isolated during a screening for biosurfactants among n-alkane degrading microorganisms. one strain-identified as alcaligenes sp. mm1-produced a novel glucose lipid. in the case of arthrobacter sp. ek 1 the well-known trehalose tetraester was found as major component. from another pure culture classified as arthrobacter sp. si 1, extracellular emulsifying agents with properties indicating high molecular weight substances were detected. furthermore tre ... | 1991 | 1878106 |
| marine biosurfactants, iii. toxicity testing with marine microorganisms and comparison with synthetic surfactants. | eight synthetic and nine biogenetic surfactants were tested on their toxicity. because of their possible application as oil dispersants against oil slicks on sea, the test organisms used were marine microorganisms (mixed and pure cultures of bacteria, microalgae, and protozoa). bacterial growth was hardly effected or stimulated, whilst that of algae and flagellates was reduced. all substances tested were biodegraded in sea water. the bioluminescence of photobacter phosphoreum (microtox test) was ... | 1991 | 1878108 |