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[characterization by rflp of dnas from campylobacter jejuni lior serotype reference strains and clinical isolates and detection of c. jejuni by dna-probe].the lior serotype reference strains and clinical isolates of campylobacter jejuni were compared in the restriction fragment length polymorphism (rflp) pattern to distinguish c. jejuni strains. these reference strains showed rflp patterns different from one to another, while the patterns of some isolates were not coincident with those of the same serotype reference strains. furthermore, we tried to hybridize hindiii-digested fragments from these strains with the dna probe encoding the 46-kda prot ...19957474356
cloning of the helicobacter pylori reca gene and functional characterization of its product.the reca protein is a key enzyme involved in dna recombination in bacteria. using a polymerase chain reaction (pcr) amplification we cloned a reca homolog from helicobacter pylori. the gene revealed an open reading frame (orf) encoding a putative protein of 37.6 kda showing closest homology to the campylobacter jejuni reca (75.5% identity). a putative ribosome binding site and a near-consensus sigma 70 promoter sequence was found upstream of reca. a second orf, encoding a putative protein with n ...19957476856
campylobacter jejuni infection and guillain-barré syndrome.although infection with campylobacter jejuni is recognized as a common antecedent of the guillain-barré syndrome, the clinical and epidemiologic features of this association are not well understood.19957477117
anti-ganglioside gm1 antibodies in guillain-barré syndrome and their relationship to campylobacter jejuni infection.to clarify the association between campylobacter jejuni (cj) infection and antibodies to ganglioside gm1 (anti-gm1) in guillain-barré syndrome (gbs), we have carried out a prospective case-control study of 96 patients with gbs. cj infection occurred in 25 (26%) patients. igg and/or igm anti-gm1 were identified in 24 (25%) patients and in 1 of 71 (1.4%) household controls (p < 0.001). thirteen of the 25 (52%) cj-positive patients had anti-gm1 compared with 11 of the 71 (15%) cj-negative patients ...19957486873
partial characterization and effect of omeprazole on atpase activity in helicobacter pylori by using permeabilized cells.atpase activity in permeabilized cells of helicobacter pylori as well as those of helicobacter felis and campylobacter jejuni was analyzed. the atpase activities in these cells were most susceptible to sodium azide, fluoroaluminate, and dicyclohexylcarbodiimide, which are typical inhibitors of f atpases. optimal values for maximal activity were found to be at approximately ph 6.4, 6.0, and 6.0 for c. jejuni, h. pylori, and h. felis, respectively. the substituted benzimidazole compounds omeprazol ...19957486907
production of monoclonal antibody specific to campylobacter jejuni and its potential in diagnosis of campylobacter enteritis.a monoclonal antibody (mab 3g6) specific for campylobacter jejuni and campylobacter coli was produced by immunizing balb/c mice with a local strain of c.jejuni (28.1). no cross-reactivity was observed with enterobacteriaceae controls. by immunoprecipitation, mab 3g6 identified a major protein band of molecular weight 45 kda and also gave a slight reactivity with 30 and 55 kda proteins. using an indirect enzyme-linked immunosorbent assay, mab 3g6 was able to detect c.jejuni suspended in stool wit ...19957488346
the isolation of campylobacter jejuni from contaminated surfaces and its survival in diluents.the isolation rates of campylobacters from contaminated surfaces were improved if swabs were placed directly into selective media rather than being stored in diluents before culture. storage in diluents resulted in a loss of viability and the remaining viable campylobacter cells were often sub-lethally injured which sensitised them to selective agents in culture media and reduced isolation rates. campylobacter jejuni, suspended in small drops of blood, was capable of prolonged survival on work s ...19957488525
diffusion of thermophilic campylobacter in the pesaro-urbino area (italy) from 1985 to 1992.the results of research on the spreading of campylobacter in the pesaro-urbino area carried out from 1985 to 1992 are presented. materials of different origin were examined: 822 samples of human faeces, 533 animal rectal swabs, 192 samples of domestic sewage, 48 of river water, 96 of sea water and 632 of various types of food. two hundred and nine strains of campylobacter were isolated (9%), most of which were campylobacter jejuni (80%), with particular frequency in food products (chicken carcas ...19957489778
identification of campylobacter jejuni and c. coli using the rpob gene and a cryptic dna fragment from c. jejuni.campylobacter jejuni (cj) and c. coli (cc) clinical isolates, obtained from three different sources, were characterized using two cj dna probes, cj01 and cj02. these probes were selected at random by virtue of their stability in escherichia coli (ec). cj01 hybridized specifically with dna from cj reference strains, but not with dna from cc, c. lari (cl) nor c. fetus (cf) reference strains. using clinical isolates characterized by genome-genome hybridization and biotype, cj01 hybridized with dna ...19957489896
guillain-barré syndrome in northern china. relationship to campylobacter jejuni infection and anti-glycolipid antibodies.guillain-barré syndrome has been considered to be primarily an acute inflammatory demyelinating polyneuropathy (aidp). our experience with guillain-barré syndrome in northern china differs from the traditional concept. electrophysiologically and pathologically, most of our patients have motor axonal degeneration with minimal cellular inflammation, which we have termed 'acute motor axonal neuropathy' (aman). the current studies were undertaken to characterize prospectively the clinical, electroph ...19957600081
two novel restriction endonucleases from campylobacter jejuni.we have discovered two unusual restriction endonuclease (enases) in two campylobacter jejuni strains that recognize asymmetrical, interrupted sequences and cleave the dna both before and after their recognition sites. both enzymes require adomet as a cofactor for their enase activity.19957607469
campylobacter jejuni in the stomach.campylobacter jejuni is the commonest cause of acute bacterial enteritis in the uk. however, in this case a 74-year-old lady underwent gastroscopy for an upper gastrointestinal haemorrhage and was noted to have a gastric ulcer. gastric biopsy revealed spiral gram-negative bacteria and culture yielded a moderate growth of c. jejuni. identification was confirmed by growth characteristics, biochemical tests and pcr amplification of the species-specific flagellin gene--fla a. to prevent misidentific ...19957608961
[a case of guillain-barré syndrome with ophthalmoplegia showing high titers of anti-gq1b and -gd1b antibodies in the serum].a 24-year-old housewife developed double vision, tingling sensation, and weakness in the legs following a flu-like illness. she then developed dysphagia and difficulty in standing. on admission her eyes were fixed in midline due to ophthalmoplegia. doll's eye sign was negative. she had severe generalized muscle weakness and no sensory deficits. all tendon reflexes were lost. csf protein was 58 mg/dl with cell count of 2/mm3. antibodies to campylobacter jejuni, mycoplasma, ebv, and other microbes ...19957614756
[fisher's syndrome following campylobacter jejuni enteritis--a case report and review of the literature].a 13-year-old boy presented with ophthalmoplegia, cerebella ataxia and areflexia subsequent to watery diarrhea and pyrexia. campylobacter jejuni (pen 2: lio 4) was isolated from the stools. the patient had a high titer of igg anti-gq1b igg antibody titers which decreased during the clinical course. although c. jejuni has recently been recognized as the most common microorganism causing infection preceding guillain-barré syndrome, only six patients with fisher's syndrome after this bacterial infe ...19957614765
differentiation of campylobacter jejuni and campylobacter coli strains by using restriction endonuclease dna profiles and dna fragment polymorphisms.the chromosomal dna fragment patterns from a total of 169 campylobacter jejuni and campylobacter coli isolates from poultry and humans were analyzed by using dna restriction endonucleases clai and ecorv. the dna restriction patterns produced by clai and ecorv consisted of unique dna fragments of 9 to 9.5 kb and 3.5 kb generated with clai and a single unique fragment of 3.0 kb produced by ecorv. these patterns were obtained with all strains of c. jejuni tested. the dna restriction patterns were f ...19957615717
temperature-dependent membrane fatty acid and cell physiology changes in coccoid forms of campylobacter jejuni.the effect of temperature and the availability of nutrients on the transition of spiral campylobacter jejuni cells to coccoid forms was investigated. ageing of spiral c. jejuni cells in either nutrient-poor or nutrient-rich environments resulted in the formation of nonculturable coccoid cells at 4, 12, and 25 degrees c after different periods, with the cells incubated at 4 degrees c in nutrient-deficient media remaining culturable the longest. to study the phenomenon, atp levels, protein profile ...19957618883
campylobacter jejuni and the expanding spectrum of related infections. 19957619982
anti-ganglioside antibodies in patients with guillain-barré syndrome and campylobacter jejuni infection. 19957622918
[comparison by ses-page of molecular weights of lipopolysaccharides from campylobacter jejuni lior serotype reference strains and clinical isolates].to compare the molecular weights (mws) of lipopolysaccharides (lpss) from 30 lior serotype reference strains and 17 clinical isolates of campylobacter jejuni, we analyzed their migration rates by sds-page and the silver staining of the gel. lpss from the serotype strains showed one band in the low-molecular-weight region of the gel as did those from r mutants of enterobacteria. based on those from salmonella minnesota r mutants, mws of lpss from c. jejuni strains were calculated to fall within a ...19957623421
biological and serological characterization of campylobacter jejuni lipopolysaccharides with deviating core and lipid a structures.lipopolysaccharides from campylobacter jejuni were tested for their ability to induce toxic lethality in galactosamine-sensitized mice, pyrogenicity in rabbits and tumour necrosis factor (tnf) secretion from mouse peritoneal macrophages. compared with those of salmonella lps, lethal toxicity was 50% lower, pyrogenicity was 30- to 50-fold lower, and ability to induce tnf was 100-fold lower. c. jejuni lps and lipid a exhibited higher phase-transition temperatures than those of salmonella preparati ...19957640672
immunogenicity and protective efficacy of a prototype campylobacter killed whole-cell vaccine in mice.the immunogenicity and efficacy of an experimental inactivated campylobacter jejuni whole-cell (cwc) vaccine were evaluated in mice. mice were orally immunized in a three-dose primary series (48-h intervals) at doses of 10(5), 10(7), or 10(9) cwc vaccine particles alone or in combination with 25 micrograms of a mucosal adjuvant, the heat-labile enterotoxin of escherichia coli (lt). the comparative immunogenicities of both formulations were assessed on the basis of the generation of antigen-speci ...19957642317
purification and characterization of ferritin from campylobacter jejuni.we purified an iron-containing protein from campylobacter jejuni using ultracentrifugation and ion-exchange chromatography. electron microscopy of this protein revealed circular particles with a diameter of 11.5 nm and a central core with a diameter of 5.5 nm. the protein was composed of a single peptide of 21 kda and did not serologically cross-react with horse spleen ferritin. the uv-visible spectrum of the protein showed no absorption peaks in the visible region, indicating that little or no ...19957646314
[infectivity of campylobacter jejuni and campylobacter coli in chickens].the infectivity of four campylobacter (c.) jejuni strains and one c. coli strain was evaluated by the reisolation rate from cloacal swabs after oral infection of chickens at four weeks of age. one strain did not colonize the digestive tract, another infected only a part of the experimental group and a third was excreted at a lower percentage in the first two weeks p.i. compared to the other two strains. in correlation to age, the excretion rate was highest in 7-week-old birds. in groups of 4 and ...19957646423
[epidemiological investigation of campylobacter jejuni infection in children].epidemiological studies on campylobacter jejuni infection in children were carried out in chengdu during december 1987 to october 1989. detection rate of campylobacter in 1,092 children with diarrhea was 11.36%, with the highest in children aged 1-2 and the lowest in babies within six months. campylobacter jejuni could be detected in young children with acute diarrhea all the year round, with a higher detection rate in the spring and summer and lower in the autumn and winter. campylobacter jejun ...19957648948
rapid detection of campylobacter jejuni and campylobacter coli isolated from clinical specimens using the polymerase chain reaction.seventeen campylobacter strains isolated from 16 children hospitalised with acute diarrhea were analysed by in vitro enzymatic amplification using two sets of oligonucleotide primers specific for campylobacter jejuni and campylobacter coli, respectively. thirteen strains (76%) were identified as campylobacter jejuni and four strains (24%) as campylobacter coli. subsequent bacteriological identification confirmed the identity of the same 13 campylobacter jejuni strains and the 4 campylobacter col ...19957649203
[the complement fixation reaction: a traditional, updated method for the detection of antibodies in the diagnosis of infections].complement-fixation (cf) is still an important basic serologic test for the diagnosis of infectious diseases. in several areas of microbiology (viral, bacterial, parasitic and fungal) it has served as a reference standard against which other methods have been compared. its partial displacement by other techniques, as advocated in some recent literature, is often unfounded and uncritical; displacement is mostly due not to problems inherent in the method, but rather to the lack of reagents of sati ...19957651069
anti-gm1 igg antibodies and campylobacter bacteria in guillain-barré syndrome: evidence of molecular mimicry.in guillain-barré syndrome antibodies to gm1 and the presence of an antecedent campylobacter jejuni infection are correlated with a more severe course of the disease. from a group of 137 consecutive gbs patients, 11 sera had elevated titers of anti-gm1 igg antibodies during the acute stage of disease. each serum sample was preincubated with three different penner serotypes of whole c. jejuni (pen o:4/59, pen o:41) and campylobacter coli (pen o:22) bacteria. the pen o:4/59 serotype, isolated from ...19957654064
guillain-barré syndrome without sensory loss (acute motor neuropathy). a subgroup with specific clinical, electrodiagnostic and laboratory features. dutch guillain-barré study group.we analysed data obtained from 27 out of a group of 147 patients with guillain-barré syndrome, who did not have sensory loss during a follow-up period of 6 months (motor guillain-barré syndrome). these patients had a distinctive clinical pattern compared with the other 120 guillain-barré syndrome patients. the clinical course was marked by a more rapid onset of weakness (3.9 versus 6.1 days, p = 0.002), an earlier nadir (6.3 versus 9.1 days, p < 0.001), an initially predominant distal weakness ( ...19957655882
a survey of campylobacter bacteremia in three danish counties, 1989 to 1994.over a 5-year period (april 1989 to march 1994) bacteremia caused by campylobacter species was diagnosed in 15 patients in a population of 1.3 million in three counties in jutland province, denmark. the incidence was 0.2/100,000/year and the ratio of blood to fecal isolates, was 0.008. campylobacter coli and campylobacter jejuni accounted for 6 cases each, campylobacter fetus for 2, and in 1 case the species diagnosis was uncertain. seven patients were males and 8 females; median age was 42 (ran ...19957660078
multiple abscesses of the liver caused by campylobacter jejuni.a 37-year-old woman developed multiple liver abscesses caused by campylobacter jejuni, as a consequence of unrecognized and inadequately treated campylobacter enteritis. the diagnosis was established by isolation of campylobacter jejuni from blood and pus obtained from one of the liver abscesses during laparoscopy. the abscesses were successfully treated with intravenously and orally administrated antibiotic drugs, without further percutaneous drainage.19957665820
sepsis in children with human immunodeficiency virus infection. the madrid hiv pediatric infection collaborative study group.the aims of this retrospective study were to review the frequency and patterns of bacterial sepsis in children infected with human immunodeficiency virus. the charts of 233 human immunodeficiency virus-infected children cared for during a 10-year period in 4 tertiary hospitals in madrid were reviewed. there were 43 episodes of sepsis in 31 (13%) children. twenty of them had acquired immunodeficiency syndrome, 10 were class pa2 and 1 was class p1b. the most common organisms recovered were: nontyp ...19957667058
the gene for campylobacter trigger factor: evidence for multiple transcription start sites and protein products.a gene encoding a protein of apparent molecular mass 56 kda that shares 31% identity with the amino acid sequence of trigger factor from escherichia coli (a protein thought to be involved in cell division), was cloned from campylobacter jejuni nctc 11168. the clone was selected from a lambda zap ii genomic dna library following an immuno-screen using antiserum raised against glycine-extractable proteins from c. jejuni. the gene has two potential initiation codons, giving rise to two possible nes ...19957670637
molecular characterization of kata from campylobacter jejuni and generation of a catalase-deficient mutant of campylobacter coli by interspecific allelic exchange.a gene encoding catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase; ec 1.11.1.6) from campylobacter jejuni was cloned by functional complementation of a catalase-deficient mutant of escherichia coli. the catalase structural gene, designated kata, was assigned by subcloning and its nucleotide sequence determined. the deduced protein product of 508 amino acids, which had a calculated molecular mass of 58,346 da, was found to be structurally and enzymically similar to hydrogen-peroxidases ...19957670638
hla-class ii alleles in guillain-barré syndrome and miller fisher syndrome and their association with preceding campylobacter jejuni infection.hla typing for class ii alleles was performed on 97 patients with guillain-barré syndrome or miller fisher syndrome and compared with 100 controls. there was a significant association between hla-dqb1*03 and preceding campylobacter jejuni infection (pc = 0.05).19957499492
serum anti-gq1b igg antibodies recognize surface epitopes on campylobacter jejuni from patients with miller fisher syndrome.three patients who had diarrhea prior to the development of miller fisher syndrome are presented. campylobacter jejuni was isolated from stool specimens from all patients. high titers of anti-gq1b igg antibodies were demonstrated in the serum of these patients by enzyme-linked immunosorbent assay and thin-layer chromatography overlay. in enzyme-linked immunosorbent assay inhibition studies the anti-gq1b igg antibodies bound specifically to whole bacteria of the miller fisher syndrome-associated ...19957531419
nucleotide sequence of the gyra gene and characterization of ciprofloxacin-resistant mutants of helicobacter pylori.pcr was used to amplify a 238-bp region from helicobacter pylori which corresponded to the quinolone resistance-determining region in escherichia coli. the gyra gene of h. pylori was cloned and sequenced. an open reading frame of 2,478 nucleotides coded for a polypeptide of 826 amino acids with a calculated molecular mass of 92,508 da. the amino acid sequence showed an overall 52% identity with other bacterial gyra genes but was most closely related to the gyra subunit of campylobacter jejuni (7 ...19957695290
interleukin-8 response of gastric epithelial cell lines to helicobacter pylori stimulation in vitro.gastric infection with helicobacter pylori activates a mucosal inflammatory response by mononuclear cells and neutrophils that includes expression of cytokines interleukin-1 beta (il-1 beta), il-6, tumor necrosis factor alpha, and il-8. in this study, we analyzed the il-8 response of human gastric cancer cell lines (kato iii, ags, and mkn28) to h. pylori infection in vitro. il-8 mrna expression was detected by reverse transcription-pcr amplification of rna extracted from epithelial cells after i ...19957729872
molecular characterization of a campylobacter jejuni lipoprotein with homology to periplasmic siderophore-binding proteins.a genomic library of campylobacter jejuni (nctc 11351) was used to identify genes which could confer a hemolytic phenotype to escherichia coli. accordingly, when transformants were screened on blood plates, hemolytic colonies appeared at a frequency of 3 x 10(-4). the gene conferring the hemolytic activity was identified by subcloning and was found to be responsible for the phenotype of all hemolytic transformants isolated. the open reading frame conferring this activity encodes a protein of 36, ...19957730251
expression and characterization of campylobacter jejuni benzoylglycine amidohydrolase (hippuricase) gene in escherichia coli.the basis for the difference between campylobacter jejuni and campylobacter coli is the presence and expression of the n-benzoylglycine amidohydrolase (hippuricase) gene only in c. jejuni. a pbr322 recombinant clone (phip-o) of c. jejuni tgh9011 capable of converting hippuric acid into benzoic acid and glycine, the hallmark of hippuricase activity, was characterized and sequenced. the hippuricase gene (hipo) was identified by use of deletion subclones and insertional inactivation. the transcript ...19957730270
igg anti-gm1 antibodies from patients with acute motor neuropathy are predominantly of the igg1 and igg3 subclasses.increased titers of igg anti-gm1 and anti-asialo gm1 (ga1) ganglioside antibodies are present in some patients with the guillain-barré syndrome, particularly with the motor axonal variant, and following infection with campylobacter jejuni or parenteral administration of gangliosides. the subclass distribution of igg anti-gm1 or ga1 antibodies from 19 patients with acute motor neuropathy and elevated antibody titers were measured by elisa using mouse monoclonal antibodies specific for human igg s ...19957730449
[pharmacokinetic, bacteriological and clinical studies of sy5555 in the pediatric field].pharmacokinetic, bacteriological and clinical studies on sy5555, a new oral penem, were carried out, and the following results were obtained. 1. mics were determined for 6 drugs, sy5555, clavulanic acid/amoxicillin (cva/ampc), cefaclor (ccl), cefotiam (ctm), cefpodoxime (cpdx), cefdinir (cfdn) against 20 strains of bacteria isolated from patients who were subsequently treated with sy5555. mics of sy5555 for gram-positive cocci ranged from 0.05 to 0.10 microgram/ml against 10 strains of staphyloc ...19957745814
detection of campylobacter jejuni added to foods by using a combined selective enrichment and nucleic acid sequence-based amplification (nasba).an assay to detect campylobacter jejuni in foods that uses a short selective enrichment culture, a simple and rapid isolation procedure, nasba amplification of rna, and a nonradioactive in solution hybridization was studied. the presence of high numbers of indigenous flora affected the sensitivity of the assay. however, detection of c. jejuni was possible up to a ratio of indigenous flora to c. jejuni of 10,000:1. interference by food components was eliminated by centrifugation following the enr ...19957747955
detection of alpha- and beta-hemolytic-like activity from campylobacter jejuni.alpha-hemolytic-like activity from campylobacter jejuni was clearly apparent when the medium ph ranged from 6.0 to 6.5, but the hemolytic zones disappeared when the ph of the medium increased. beta-hemolytic-like activity just beneath the bacterial growth appeared after prolonged incubation. the hemolytic activity was not influenced by the species of blood.19957751385
differential clinical and epidemiological features in children with campylobacter diarrhoea, mixed-agent diarrhoea and campylobacter diarrhoea plus parenteral infections.a prospective study of 111 young peruvian children with campylobacter jejuni diarrhoea showed that it behaves as an endemic enterotoxigenic-like, waterborne, milkborne, and zoonotic disease. although there were no definite differential features between pure c. jejuni diarrhoea, mixed-agent diarrhoea, and c. jejuni diarrhoea plus parenteral infections, children with c. jejuni diarrhoea plus parenteral infections were all inpatients, were more frequently malnourished and more frequently exhibited ...19957723135
susceptibilities of fluoroquinolone-resistant strains of campylobacter jejuni to 11 oral antimicrobial agents.the resistance of campylobacter jejuni strains to the fluoroquinolones is increasingly frequent, and in our area it reaches nearly 50%. we studied the susceptibilities of 60 of these strains to 11 oral antibiotics. all strains except one were susceptible to the macrolides tested, with azithromycin being the most active agent tested. of the rest of the antibiotics studied, amoxicillin-clavulanic acid, clindamycin, and fosfomycin displayed good in vitro activities. knowledge of the susceptibilitie ...19957726529
calcium and protein kinase c play an important role in campylobacter jejuni-induced changes in na+ and cl- transport in rat ileum in vitro.the pathophysiological mechanism of campylobacter jejuni (enterotoxigenic) induced secretory diarrhoea remains least understood. to investigate the mechanism(s) involved, the unidirectional fluxes of na+ and cl- were measured across the c. jejuni live culture infected and control (non infected) rat ileum (unstriped), in vitro by ussing technique under short circuit conditions, in the presence or absence of: ca2+ ionophore a23187 (5 microm), 1-verapamil (100 microm), calmodulin (cam) antagonist w ...19957727542
[detection of imipenem resistance in a strain of campylobacter jejuni isolated from feces of a boy with diarrhea]. 19957711121
identification of helicobacter pylori by immunological dot blot method based on reaction of a species-specific monoclonal antibody with a surface-exposed protein.monoclonal antibodies (mabs) against membrane preparations of helicobacter pylori were produced. one mab was found to be specific for h. pylori, because it did not react with a number of other bacterial species, including helicobacter felis and campylobacter jejuni. this mab reacted with a 30-kda protein found in outer membrane preparations of h. pylori. the protein was also detected on the cell surface on intact bacteria when analyzed by immunoelectron microscopy. to facilitate the identificati ...19957714196
isolation of the helicobacter pylori reca gene and involvement of the reca region in resistance to low ph.to understand the potential roles of the important dna repair protein reca in helicobacter pylori pathogenesis, we cloned the reca gene from h. pylori 84-183. degenerate pcr primers based on conserved reca protein regions were used to amplify a portion of h. pylori reca, which was used as a probe to isolate the full-length reca gene from h. pylori genomic libraries. the h. pylori reca gene encoded a protein of 347 amino acids with a molecular mass of 37.6 kda. as expected, h. pylori reca was hig ...19957768597
incidence and transmission of antibiotic resistance in campylobacter jejuni and campylobacter coli.one-hundred and two campylobacter clinical isolates were characterized for their in-vitro resistance to erythromycin (1.9%), three fluoroquinolones (31.3-34.3%), tetracycline (43.1%), kanamycin (4.8%), ampicillin (18.6%) and other 16 antimicrobial agents. conjugative transfer of tetracycline and kanamycin resistances among these strains was achieved and small plasmids of 4.3, 4 and 1.9 kb were observed in kanamycin-resistant campylobacter coli strains.19957768766
[guillain-barré syndrome associated with anti-galnac-gd1a antibody subsequent to campylobacter jejuni enteritis].we report a 15-year-old man with guillain-barré syndrome (gbs) following campylobacter jejuni enteritis. neurologic examination revealed distal muscle weakness and mild disturbance of superficial sense on right plantar area. serial electrophysiologic studies indicated that the predominant process was axonal degeneration of motor nerves. thin-layer chromatography with immunostaining revealed that serum igg strongly reacted with galnac-gd1a and weakly with gm2, but did not react with gm1. this is ...19957781242
restriction fragment length polymorphisms among the flagellar genes of the lior heat-labile serogroup reference strains and field strains of campylobacter jejuni and c. coli.several typing systems have been described for campylobacter jejuni and c. coli, to assess the complex epidemiology of these important enteric pathogens. in the present study two typing methods, slide agglutination according to the lior scheme, and the demonstration of restriction-fragment length polymorphisms (rflp) of flagellar genes, have been used in parallel on a set of 194 strains. this set comprised 118 sero-reference strains of c. jejuni and c. coli of the lior scheme, as well as 76 clin ...19957781730
the fate of potentially pathogenic bacteria in swiss hard and semihard cheeses made from raw milk.this study examined the ability of potentially pathogenic bacteria to grow and to survive during the manufacture and ripening of swiss hard and semihard cheese varieties made from raw milk. the results show that hard cheeses are hygienically safe; 1 wk after fabrication, the inoculated pathogens (aeromonas hydrophila. campylobacter jejuni, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella spp., staphylococcus aureus, and yersinia enterocolitica) could no longer be dete ...19957782504
[a case from practice (326). dysentery (inflammatory diarrhea, cause campylobacter jejuni]. 19957784778
[peripheral neuropathies].some new aspects of peripheral nerve disease are reviewed. hereditary motor and sensory neuropathy (hsmn) constitutes a genetically heterogeneous group of chronic polyneuropathies with loci mapping to chromosome 17 (type ia), chromosome 1 (type ib), and the x chromosome (x-linked hsmn). although peripheral nerve abnormalities occur in approximately 50% of diabetic patients, the frequency of severe diabetic neuropathy even in insulin-dependent persons is rather modest (6%). intensified insulin tr ...19957785034
sensitivity of campylobacter spp. to irradiation in poultry meat.the sensitivity of campylobacter jejuni (three strains), camp. coli (three strains), camp. fetus (one strain) and camp. lari (one strain) to irradiation in poultry meat was investigated. there was no significant difference in the counts obtained on blood or skirrows agar. preston agar gave a significantly lower recovery of the pathogens after irradiation so these results were not included in calculations of d10 values. the d10 values ranged from 0.12 to 0.25 kgy and there was a significant diffe ...19957786498
discrepancy between penner serotyping and polymerase chain reaction fingerprinting of campylobacter isolated from poultry and other animal sources.thirty-four campylobacter jejuni or coli strains, isolated from various livestock and darkling beetles from two dutch poultry farms during different broiler production cycles, were subjected to penner serotyping and polymerase chain reaction (pcr) fingerprint analysis. ten different penner serotypes were determined in the isolates. visual scoring of the pcr fingerprints resulted in 14 clearly different profiles. some strains with identical penner serotypes exhibited different pcr fingerprints an ...19957786504
prevalence of campylobacter enteritis in children from yaounde (cameroon).a cross sectional study on the prevalence of campylobacter jejuni/coli and its possible aetiologic role in childhood diarrhoea in yaounde was undertaken in 272 children presenting with diarrhoea and 157 age matched controls from april 1989 to october 1990. stool cultures were performed according to standard techniques for campylobacter jejuni/coli shigella and salmonella species. rotavirus was detected using a latex agglutination test. campylobacter jejuni/coli was isolated from 21 (7,7 pc) of 2 ...19957788677
campylobacter jejuni arthritis in secondary amyloidosis.we describe a case of infectious arthritis caused by c. jejuni which developed after c. jejuni enteritis in a patient with rheumatoid arthritis and hypogammaglobulinaemia. extraintestinal infections of this enteropathogen are rare, but in patients with hypogammaglobulinaemia c. jejuni bacteriaemia is relatively common. the present patient suffered from hypogammaglobulinaemia due to a nephrotic syndrome in secondary amyloidosis. c. jejuni infection must be considered in patients with hypogammaglo ...19957789064
gm1 antibody in guillain-barre syndrome after campylobacter jejuni infection.fecal culture of campylobacter jejuni was prepared by the method of skirrow, and serum class specific antibodies (igg, igm and iga) to campylobacter jejuni and serum class specific antibodies (igg igm) to gm1 were prepared with solid phase enzyme linked immunasorbent assay in 16 cases of guillain-barre syndrome (gbs), 32 controls with other neurological diseases (disease controls) and 90 normal controls. the results showed that the incidence of campylobacter jejuni infection, especially recent i ...19957789212
identification of campylobacter jejuni on the basis of a species-specific gene that encodes a membrane protein.to facilitate discrimination between the closely related enteropathogens campylobacter jejuni and c. coli, unique differences in antigenic surface structure were examined. a genomic library of c. jejuni 81116 was constructed in plasmid pbluescriptiisk- and expressed in escherichia coli k-12. rabbit hyperimmune serum raised against c. jejuni atcc 29428 recognized a clone expressing a c. jejuni 24-kda membrane-associated protein. antiserum raised against sonicated recombinant e. coli expressing th ...19957790451
immunopathogenesis and treatment of the guillain-barré syndrome--part i.the etiology of the guillain-barré syndrome (gbs) still remains elusive. recent years have witnessed important advances in the delineation of the mechanisms that may operate to produce nerve damage. evidence gathered from cell biology, immunology, and immunopathology studies in patients with gbs and animals with experimental autoimmune neuritis (ean) indicate that gbs results from aberrant immune responses against components of peripheral nerve. autoreactive t lymphocytes specific for the myelin ...19957823972
immunopathogenesis and treatment of the guillain-barré syndrome--part ii.in the second part of our review the role of antecedent infections in the pathogenesis of gbs is discussed. the association with campylobacter jejuni (c. jejuni) is highlighted and the concept of molecular mimicry, i.e., sharing of epitopes between microbes and peripheral nerve, explained. alternative mechanisms to relate an infection with the immune-mediated neuropathy are elaborated. current therapies of the gbs include plasma exchange, high-dose intravenous immunoglobulins, and supportive tre ...19957823973
familial guillain-barré syndrome subsequent to campylobacter jejuni enteritis. 19957815216
fisher syndrome after campylobacter jejuni enteritis: human leukocyte antigen and the bacterial serotype.we describe two children who had fisher syndrome subsequent to campylobacter jejuni enteritis. the c. jejuni isolates from both patients, who lived in different areas, belonged to pen 2: lio 4. one patient had the following human leukocyte antigens (hlas): hla-a24, 33; b44, 52; dq1; and dr2, 6 antigens. another had the hla-a24, 33; b44, 54; cw1; dq1, 4; and dr4, 6. an effort should be made to isolate c. jejuni from patients with fisher syndrome and to perform hla typing so that the pathogenesis ...19957815224
cross-reactive antigens shared by pseudomonas aeruginosa, helicobacter pylori, campylobacter jejuni, and haemophilus influenzae may cause false-positive titers of antibody to h. pylori.cystic fibrosis (cf) patients suffer from many of the gastrointestinal conditions which occur in non-cf individuals, e.g., dyspepsia and peptic ulceration. these symptoms may be caused by helicobacter pylori but could also be due to either pancreatic insufficiency or the intensive antibiotic treatment used in cf patients. since cf patients chronically infected with pseudomonas aeruginosa produce antibodies against a wide range of antigens, including antigens common to many other bacteria, e.g., ...19957697522
expression of interleukin 8 and cd54 by human gastric epithelium after helicobacter pylori infection in vitro.helicobacter pylori is associated with neutrophil infiltrates, although the mechanism of their recruitment is only partially defined. the aim of the study was to determine if kato iii, a human gastric epithelial cell line, expressed cytokines and the intercellular adhesion molecule 1 (icam-1), which could contribute to the initiation of inflammation during infection with h. pylori.19957806065
polymerase chain reaction (pcr) for detection of pathogenic microorganisms in bacteriological monitoring of dairy products.the presence of pathogenic bacteria poses a serious problem in sustaining the safety of dairy products. microbiological routine controls of these products make use of selective culture techniques. to detect pathogenic species, isolated colonies are characterized by specific metabolic activities and by serotyping. we present an alternative biochemical approach that does not require culture of bacteria. the total bacterial populations of food samples were isolated by centrifugation and analysed by ...19957754231
specific detection of campylobacter jejuni by means of polymerase chain reaction in chicken litter.a routine detection assay of campylobacter jejuni was developed with the polymerase chain reaction (pcr). a pair of oligonucleotide primers from the flaa of c. jejuni were used in detection by pcr. although the primer pair specifically detected c. jejuni strains, they did not detect strains of c. coli, c. fetus, salmonella spp. or escherichia coli. in litter inoculated with c. jejuni, specific detection of c. jejuni was obtained by pcr with this primer pair.19957756404
development of a pcr assay combined with a short enrichment culture for detection of campylobacter jejuni in estuarine surface waters.two extraction procedures were examined, and it was found that dna recovered from campylobacter jejuni lysed by the cetyltrimethylammonium bromide (ctab) method was more suitable for use as a pcr template than dna released by the boiling method. the region targeted for pcr amplification was a 1.73-kb portion of the flagellin a gene of c. jejuni. the detection limit was lower than 30 cells per 100 ml in artificially contaminated waters. pcr assay and conventional culturing method had the same sen ...19957758934
resistance to erythromycin in fluoroquinolone-resistant campylobacter jejuni strains isolated from human faeces. 19957759401
[miller-fisher syndrome. role of campylobacter jejuni infection]. 19957761368
[infections caused by campylobacter jejuni in the pediatric department of the leoben district hospital 1983-1993].over the period 1983-1993 10,344 stool cultures were undertaken in children with gastrointestinal symptoms at the pediatric department of the landeskrankenhaus leoben. campylobacter jejuni was diagnosed as pathogen in 238 cultures taken from 196 patients aged one month to 16 years. thus, campylobacter jejuni infections (2.3% of all stool cultures) were second in frequency to various forms of salmonella (6.1% of all stool cultures; 332 patients). apart from diarrhea (92% of cases), the most frequ ...19957762243
practice guidelines for ordering stool cultures in a pediatric population. alberta children's hospital, calgary, alberta, canada.a comprehensive utilization review was done of all stool culture tests performed at a pediatric hospital during a 3-year period from june 1, 1989 through to july 1, 1992. a total of 4,460 stool culture specimens were surveyed from 3,420 children. sixty percent (2,692) of the workload was from inpatients, 22% (1,001) was from emergency room (er) visits and 18% (767) was from outpatient clinic/office visits. a total of 9% (294 of 3,420) of the children were confirmed to have enteric bacterial infe ...19957856556
campylobacter hyoilei sp. nov., associated with porcine proliferative enteritis.campylobacter hyoilei sp. nov. is the name proposed for an organism formerly described as strain rmit 32at (t = type strain) and a group of similar bacteria isolated from intestinal lesions of pigs with proliferative enteritis. the phenotypic characteristics of these organisms indicated that they are closely related to each other and are not strains of other campylobacter spp. commonly isolated from pigs. the results of probing of clai-, ecorv-, or bglii-cleaved genomic dnas from c. hyoilei stra ...19957857809
intestinal carriage of campylobacters, salmonellas, yersinias and listerias in pigeons in the city of barcelona.the faecal bacterial flora of pigeons, which may be the source of infectious diseases in man, was studied in the city of barcelona. four hundred cloacal specimens were examined for campylobacter jejuni, camp. coli, salmonella spp., yersinia spp. and listeria spp., over a 12 month period. campylobacter jejuni was the most frequently isolated micro-organism, found in 105 pigeons (26.2%), with a greater incidence in the districts of the city with a high density of pigeons and without seasonal varia ...19957883642
direct milk excretion of campylobacter jejuni in a dairy cow causing cases of human enteritis.consumption of milk contaminated with campylobacter jejuni has been described as a cause of human enteritis. although faecal contamination of milk with the organism has frequently been described, direct milk excretion of campylobacter jejuni into milk has rarely been linked with cases of human infection. we describe the investigations undertaken following the isolation of campylobacter jejuni from samples of unpasteurized milk prior to retail. results of epidemiological investigations including ...19957867733
[presence of virulence factors and pathovars in strains of campylobacter jejuni isolated from patients with diarrhea and healthy children].to study the prevalence of virulence factors (adhesion, invasion, cytotoxicity and hemolytic activity) and to establish the presence of pathovars (virulence phenotype) in c. jejuni strains isolated in pediatric patients with inflammatory and secretory diarrhea and asymptomatic carriers.19958519832
nucleotide sequence and characterization of peb4a encoding an antigenic protein in campylobacter jejuni.the 29-kda protein peb4, a major antigen of campylobacter jejuni, is present in all c. jejuni strains tested and elicits an antibody response in infected patients. by screening a lambda gt11 library of chromosomal dna fragments of c. jejuni strain 81-176 in escherichia coli y1090 cells with antibody raised against purified peb4, a recombinant phage with a 2-kb insert expressing an immunoreactive protein of 29 kda was isolated. dna sequence analysis revealed that the insert contains two complete ...19958525063
efficacy of a lactic acid/sodium benzoate wash solution in reducing bacterial contamination of raw chicken.raw chicken wings inoculated with salmonella, campylobacter jejuni, listeria monocytogenes, staphylococcus aureus, or escherichia coli o157:h7 were washed in water (control) or a solution of a 0.5% lactic acid/0.05% sodium benzoate (lb) (ph 2.64) for 30 min. viable cells of pathogenic bacteria and naturally occurring psychrotrophic bacteria on wings were enumerated after 0, 2, 4, 6, and 8 days of storage at 4 degrees c. lower populations of pathogenic and psychrotrophic bacteria were detected on ...19958527331
emergence of multidrug resistance in campylobacter jejuni isolates from three patients infected with human immunodeficiency virus.single-drug resistance to tetracycline, doxycycline, erythromycin, or fluoroquinolones in campylobacter isolates recovered from humans has been documented worldwide. multidrug resistance to these antibiotics is rare in campylobacter jejuni. we report the sequential development of multidrug resistance in c. jejuni isolates from three patients who were infected with human immunodeficiency virus. multiple isolates recovered from stool specimens from these patients were ribotyped, and antibiotic sus ...19958527556
infectious gastroenteritis: are they all the same?campylobacter jejuni and salmonella spp are the most frequently cultured micro-organisms in infectious gastroenteritis among patients hospitalized at the departments of gastroenterology and geriatrics. as a whole, the hospitalized patient population with campylobacter gastroenteritis is a younger one, compared to the salmonella-infected group. both pathogens can be associated with a biochemical pancreatitis, which is usually without clinical importance. however, serious complications can occur, ...19958533526
quinolone resistance in clinical strains of campylobacter jejuni and campylobacter coli. 19958537280
bactericidal properties of campylobacter jejuni-specific immunoglobulin m antibodies in commercial immunoglobulin preparations.campylobacter jejuni is one of the most common enterocolitis-causing microorganisms worldwide. it is of particular importance in immunodeficient patients, who frequently are prone to develop extraintestinal manifestations. since these cases respond poorly to antibiotic treatment, a supportive immunomodulating therapy including the administration of c. jejuni-specific immunoglobulins would be desirable. in the present study, nine commercial immunoglobulin preparations for intravenous use were tes ...19958540699
evidence for an efflux pump in multidrug-resistant campylobacter jejuni.mechanisms of drug resistance in campylobacter jejuni were investigated. mutant strains 34pefr, which was resistant to pefloxacin (128-fold increase in the mic), and 34ctxr, which was resistant to cefotaxime (32-fold increase in the mic) and which was derived from the susceptible parent 34s, were obtained by serial passages on pefloxacin and cefotaxime gradient plates, respectively. both mutants showed cross-resistance to erythromycin, chloramphenicol, tetracycline, beta-lactams, and quinolones. ...19958540709
secretory monoclonal iga class-switch variants against bacterial enteric pathogens in bile and intestinal secretions.in a previous study we analyzed the molecular forms of monoclonal iga class-switch variants (moiga variants) and their transport into murine respiratory secretions. the aim of the present study is to characterize the transport of moiga variants into bile and intestinal secretions so that their applicability in a passive immunization model of the gut can be evaluated. different moiga variants were directly isolated from igg1 and igg2a producing hybridoma clones specific for the same surface deter ...19958541811
bilateral ptosis, tonic pupils and abducens palsies following campylobacter jejuni enteritis. 19958543096
[septic abortion in campylobacter jejuni infection].case report of a 24 year old woman ii g/i p with enterocolitis and septicaemia caused by campylobacter jejuni (c.) in the following time abortion at 16 weeks of gestation. diagnostics, therapy, review of literature.19958543136
[campylobacter jejuni and autoimmune-mediated neurologic diseases: pathogenesis of guillain-barré syndrome and fisher's syndrome]. 19958544349
mechanisms of resistance to fluoroquinolones: state-of-the-art 1992-1994.this paper gives an update on the mechanisms of bacterial resistance to fluoroquinolones. the laboratory techniques currently used to determine the mechanism(s) of resistance are outlined, including the use of restriction fragment length polymorphism and single-stranded conformational polymorphism analysis of mutations in gyra. alterations in gyra have continued to be the most reported cause of resistance, with high level resistance due to 2 or more mutations in this gene. recently, mutations in ...19958549336
variation of the flagellin gene locus of campylobacter jejuni by recombination and horizontal gene transfer.the capacity of campylobacter jejuni to generate genetic diversity was determined for its flagellar region. recombination within a genome, as well as recombination after the uptake of exogenous dna, could be demonstrated. the subunit of the flagellar filament of c. jejuni is encoded by two tandem genes, flaa and flab, which are highly similar and therefore subject to recombination. a spontaneous recombination within this locus was demonstrated in a bacterial clone containing an antibiotic-resist ...19957894725
[hemolytic-uremic syndrome after verotoxin-producing escherichia coli infection].the haemolytic uraemic syndrome, first described in 1955 by gasser, is the number one cause of acute renal failure in infants. there are three types of the haemolytic uraemic syndrome: the seasonal epidemic form with prodromic diarrhoea and generally favourable outcome which usually occurs in infants, a less typical form without signs of digestive tract involvement and no seasonal prevalence which occurs more readily in older children and sometimes in families has a less favourable prognosis, an ...19957899353
evaluation of a pcr based assay for specific detection of campylobacter jejuni in chicken washes.an assay for campylobacter jejuni based on the polymerase chain reaction was developed in our laboratory and shown to be a sensitive and specific method to identify this bacterium in pure culture. this assay was evaluated as a method to rapidly detect c. jejuni attached to chicken carcasses. chicken carcasses were sampled for pcr using three methods including pre-enrichment of the washes, direct plating of the washes and differential centrifugation of the washes prior to testing. it was found th ...19958569769
[axonal guillain-barré syndrome associated with anti-galnac-gd1a antibody subsequent to campylobacter jejuni (pen 43) enteritis].we reported a 16-year-old boy who had guillain-barré syndrome (gbs) after suffering diarrhea. campylobacter jejuni was isolated from his stool, and the serotype belonged to pen 43. neurologic examination revealed distal-dominant muscle weakness atrophy, and mild sensory disturbance. motor and sensory nerve conduction velocities were normal, but compound muscle action potentials were markedly reduced. serum from the patient had high titers of anti-falnac-gd1a antibodies. he had hla-a24, b51, drb1 ...19958665735
an outbreak of campylobacter jejuni enteritis associated with failed milk pasteurisation.this paper describes an outbreak of gastrointestinal illness affecting at least 110 people, of whom 41 had microbiological confirmation of campylobacter jejuni infection. the outbreak of infection was found to have been associated with consumption of inadequately pasteurised milk from a local dairy. the problem of enforcement of food and safety regulations when milk from dairies fails the phosphatase test is discussed. the prevalence of seroconversion to campylobacter in the community is estimat ...19958666844
polymerase chain reaction assay for detection of campylobacter coli and campylobacter jejuni in poultry meat.primers of 16s rrna and flaa genes were used to optimise a pcr technique for detecting thermotolerant campylobacters in poultry meat. different methods for crude dna extraction were also evaluated. the use of flaa primers and extraction of nucleic acid by boiling and proteinase k gave good results in the detection of campylobacter either in artificially or naturally contaminated foodstuffs. the lowest sensitivity limit for the pcr reaction was 10(1)-10(2) thermophilic campylobacter cells either ...19958672221
identification and characterization of an immunogenic outer membrane protein of campylobacter jejuni.we cloned and expressed in escherichia coli a gene encoding an 18-kda outer membrane protein (omp18) from campylobacter jejuni atcc 29428. the nucleotide sequence of the gene encoding omp18 was determined, and an open reading frame of 165 amino acids was revealed. the amino acid sequence had the typical features of a leader sequence and a signal peptidase ii cleavage site at the n-terminal part of omp18. moreover, the sequence had a high degree of similarity to the peptidoglycan-associated outer ...19958576327
the purification of a groel-like stress protein from aerobically adapted campylobacter jejuni.from plate cultures of campylobacter jejuni grown in room air a particulate protein of 62 kda was isolated by ion-exchange chromatography. the protein had a square shape from the side view but when viewed from the top it had a star-shaped structure. the molecular size of the whole particle determined by gel filtration was 850 kda which suggested the presence of 14 subunits of 62 kda in each particle. the n-terminal 37 amino residues showed more than 80% homology with the sequence of these heat s ...19958577276
binding of outer membrane preparations of campylobacter jejuni to int 457 cell membranes and extracellular matrix proteins.binding of outer membrane (om) preparations of the thermophilic campylobacter species c. jejuni to epithelial cell membranes and extracellular matrix proteins were studied in an in vitro model system using enzyme-linked immunosorbent assay. the om preparations exhibited significant binding to int 407 intestinal cell membranes. the process of adhesion was modulated by enzymatic, chemical or immunological pretreatment of the bacteria. following oxidation of the lipopolysaccharide (lps) with sodium ...19958577316
random amplified polymorphic dna fingerprinting of campylobacter jejuni and c. coli isolated from human faeces, seawater and poultry products.the polymerase chain reaction (pcr) technique was used to obtain randomly amplified polymorphic dna (rapd) profiles from 64 type and serotype reference strains and 114 isolates of campylobacter jejuni and c. coli from food, seawater and human faeces. genetic diversity was detected among the strains as a total of 118 different rapd profiles were obtained, each one containing from 4 to 11 bands between 0.30 and 1.50 kb. the discriminatory power of a random 10-mer primer (sequence 5'-caatcgccgt-3') ...19958584791
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