Publications

physical and transcription map of the 6p21.2-6p21.3 boundary region.		1996	9063640
glycopeptide mimics of mammalian man9glcnac2. ligand binding to mannan-binding proteins (mbps).	a novel and simple approach for rational design of oligosaccharide mimics has been developed. mammalian high-mannose triantennary structure man9glcnac2 has been subjected to molecular modelling using the nmr data available on structural fragments of the oligosaccharide. the analysis indicated four different low energy conformations, and the spatial arrangement of terminal disaccharides of the oligosaccharide antennae were stimulated with glycopeptides carrying disaccharides by applying weak cons ...	1996	9007273
paracoccidioides brasiliensis antigen batches from the same isolate show immunological and biochemical differences.	we investigated the occurrence of antigenic and biochemical variability among paracoccidioides brasiliensis antigen batches prepared according to the same protocol. initially (experiment #1), we analyzed two antigen lots of two human isolates (bt1 & bt2), cultured in two media (pyg: bactopeptone, yeast extract, glucose; mmm: mcveigh & morton medium) in sds-page and in two immunological tests (immunodiffusion-id and footpad swelling test-fpt). afterwards (experiment #2), we compared the antigenic ...	1996	9008879
calreticulin binds hyrna and the 52-kda polypeptide component of the ro/ss-a ribonucleoprotein autoantigen.	calreticulin (cr) is a multifunctional, calcium-binding protein that has recently been shown to bind to and promote the replication of the rubella virus genome in mammalian cells. while cr is now widely recognized as a new human autoantigen, the relationship between cr and the ro/ss-a ribonucleo-protein (rnp) autoantigen has been somewhat controversial. in this work, we demonstrate that unphosphorylated human rcr binds specifically and distinctly to in vitro transcribed forms of hyrna, the rna b ...	1996	8666824
the biotransformation of clomipramine in vitro, identification of the cytochrome p450s responsible for the separate metabolic pathways.	the aim of the study was to identify the cytochrome p450s (cyps) that catalyze the biotransformation of clomipramine in vitro. a high-performance liquid chromatography method was developed to assay n-desmethylclomipramine, 8-hydroxyclomipramine, 2-hydroxyclomipramine, 8-hydroxydesmethhylclomipramine, didesmethylclomipramine and 2-hydroxydesmethylclomipramine formed by microsomes prepared from human liver and yeast expressing human cyp1a1, 1a2, 2c8, 2c9, 2c18, 2c19, 2d6 and 3a4. there was a stati ...	1996	8667235
identifying a species-specific region of yeast tf11b in vivo.	the general transcription factor iib (tfiib) is required for rna polymerase ii transcription in eukaryotes. it provides a physical link between the tata-binding protein (tbp) and the rna polymerase and is a component previously suggested to respond to transcriptional activators in vitro. in this report, we compare the yeast (saccharomyces cerevisiae) and human forms of the protein in yeast cells to study their functional differences. we demonstrate that human tfiib fails to functionally replace ...	1996	8668181
s phase specific formation of the human rad51 protein nuclear foci in lymphocytes.	the rad51 protein, which is a homologue of the bacterial reca protein, is involved in mitotic and meiotic recombination and in repair of double-strand breaks of dna in yeast. the rad51 homologue is conserved from yeast to human. in this study, the rad51 protein was shown to be induced in peripheral blood lymphocytes (pbls) 36 h after phytohemagglutinin (pha) stimulation. immunofluorescence study revealed that the distribution of the rad51 protein in the nucleus was not uniform and focus-like sta ...	1996	8668342
identification of signalling proteins interacting with b-raf in the yeast two-hybrid system.	recent studies suggested the existence of ras/b-raf/ mek-1 complexes and a critical role for b-raf in regulating the map kinase/erks signalling pathway. we report, here, that both ras and mek-1 proteins interact physically with b-raf proteins in the yeast two-hybrid system. in addition, by screening a mouse brain cdna library, we isolated additional b-raf interacting proteins. these include three members of the 14-3-3 proteins family (eta, theta and zeta) and the mek-2 protein. we also show that ...	1996	8668348
processing of branched dna intermediates by a complex of human fen-1 and pcna.	in eukaryotic cells, a 5' flap dna endonuclease activity and a ds dna 5'-exonuclease activity exist within a single enzyme called fen-1 [flap endo-nuclease and 5(five)'-exo-nuclease]. this 42 kda endo-/exonuclease, fen-1, is highly homologous to human xp-g, saccharomyces cerevisiae rad2 and s.cerevisiae rth1. these structure-specific nucleases recognize and cleave a branched dna structure called a dna flap, and its derivative called a pseudo y-structure. fen-1 is essential for lagging strand dna ...	1996	8668533
identification of topoisomerase i as the cytotoxic target of the protoberberine alkaloid coralyne.	protoberberine alkaloids (coralyne and its derivatives), which exhibit antileukemic activity in animal models, have been shown to be potent inducers of topoisomerase (topo) i-dna cleavable complexes using purified recombinant human dna topo i. different from the structurally similar benzophenanthridine alkaloid nitidine (a dual poison of both topos i and ii), coralyne and its derivatives have marginal poisoning activity against dna topo ii. yeast cells expressing human dna topo i are shown to be ...	1996	8665516
assignment of psoriasin to human chromosomal band 1q21: coordinate overexpression of clustered genes in psoriasis.	psoriasin is an abundant low molecular weight protein in keratinocytes from psoriatic lesions. because of similarities in gene structure and expression to other genes on human chromosomal band 1q21, we hypothesized that psoriasin might also map to this region. to test this hypothesis, we identified and used a genomic lambda clone (lambda 9.2) as a probe for fluorescent in situ hybridization. lambda 9.2 detected the 1q21 region in 81% of 52 chromosomes 1 examined, although it also hybridized to a ...	1996	8618016
pub1 acts as an e6-ap-like protein ubiquitiin ligase in the degradation of cdc25.	the level of the mitotic activating tyrosine phosphatase cdc25 is regulated by both transcriptional and post-transcriptional mechanisms in the fission yeast schizosaccharomyces pombe. we have found that cdc25 is ubiquitinated and have cloned pub1, a gene which regulates this event. pub1 contains a region highly homologous to the putative catalytic domain of the human protein ubiquitin ligase e6-ap. disruption of pub1 elevates the level of cdc25 protein in vivo rendering cells relatively resistan ...	1996	8635463
stability of microsatellites and minisatellites in bloom syndrome, a human syndrome of genetic instability.	bloom syndrome (bs) is a human cancer-prone genetic disorder essentially characterized by a generalized genetic instability including a high level of sister chromatid exchanges (sces). although mutator and hyper-rec phenotypes of bs cells present analogies with those of bacteria and yeast defective in dna mismatch repair, we report that (ca)(n) microsatellite alterations are undetectable in bs cells. thus, our results suggest that the origin of bs mutator phenotype is not a major defect in dna m ...	1996	8637501
the tc21 oncoprotein interacts with the ral guanosine nucleotide dissociation factor.	tc21 is a highly oncogenic member of the ras superfamily of small gtp binding proteins. we have used the yeast two hybrid system to identify proteins that interact with an oncogenic form of the tc21 protein. cdna clones encoding the carboxy-terminal region of the ralgds protein were isolated from human b-cell and hela cdna libraries. ralgds is an exchange factor that stimulates gdp dissociation from ral, another member of the ras superfamily of proteins. the interaction between ralgds to tc21 is ...	1996	8637701
the proto-oncogene hlf and the related basic leucine zipper protein tef display highly similar dna-binding and transcriptional regulatory properties.	genes encoding transcription factors are frequently altered by chromosomal translocations in acute lymphoblastic leukemia (all), suggesting that aberrant transcriptional regulation plays a prominent role in leukemogenesis. e2a-hepatic leukemia factor (hlf), a chimeric transcription factor created by the t(17;19), consists of the amino terminal portion of e2a proteins, including two experimentally defined transcriptional activation domains (tads), fused to the hlf dna binding and protein dimeriza ...	1996	8639829
a gene mutated in x-linked myotubular myopathy defines a new putative tyrosine phosphatase family conserved in yeast.	x-linked recessive myotubular myopathy (mtm1) is characterized by severe hypotonia and generalized muscle weakness, with impaired maturation of muscle fibres. we have restricted the candidate region to 280 kb and characterized two candidate genes using positional cloning strategies. the presence of frameshift or missense mutations (of which two are new mutations) in seven patients proved that one of these genes is indeed implicated in mtm1. the protein encoded by the mtm1 gene is highly conserve ...	1996	8640223
a synaptobrevin-like gene in the xq28 pseudoautosomal region undergoes x inactivation.	the x and y chromosomes that maintain human dimorphism are thought to have descended from a single progenitor, with the y chromosome becoming largely depleted of genes. a number of genes, however, retain copies on both x and y chromosomes and escape the inactivation that affects most x-linked genes in somatic cells. many of those genes are present in two pseudoautosomal regions (pars) at the termini of the short (p) and long (q) arms of the sex chromosomes. for both pars, pairing facilitates the ...	1996	8640232
molecular cloning and expression of a multiubiquitin chain binding subunit of the human 26s protease.	s5a is a subunit of the 26s protease that binds and presumably selects multiubiquitinated proteins for destruction. we recently identified an arabidopsis protein, mbp1, that is physically, immunologically and biochemically similar to s5a from the human erythrocyte 26s protease. based upon the mbp1 cdna sequence we have now isolated a hela cell cdna coding for human s5a. the hela cdna sequence is highly similar to mbp1 and it encodes peptides obtained directly from human erythrocyte s5a. moreover ...	1996	8641424
[progress of research on tumor suppressor genes].	the multistage model of carcinogenesis during tumor progression requires that there should be consecutive genetic abnormalities of both oncogenes and tumor suppressor genes. as is true of the protein products of oncogenes, tumor suppressor proteins are found to have various cellular functions. they are involved in the regulation of adhesion, cell-cell interaction, and cytoplasmic signal transducers as well as nuclear transcription factors. the recently identified hmsh2 (human muts homolog 2) gen ...	1996	8641669
the telobox, a myb-related telomeric dna binding motif found in proteins from yeast, plants and human.	the yeast ttaggg binding factor 1 (tbf1) was identified and cloned through its ability to interact with vertebrate telomeric repeats in vitro. we show here that a sequence of 60 amino acids located in its c-terminus is critical for dna binding. this sequence exhibits homologies with myb repeats and is conserved among five proteins from plants, two of which are known to bind telomeric-related sequences, and two proteins from human, including the telomeric repeat binding factor (trf) and the predi ...	1996	8614633
structure of the phosphopeptidomannans from flocculent and non-flocculent yeast kluyveromyces lactis.	after extraction from whole cells, and purification by gel filtration, the chemical composition and molecular mass estimation of the cell-wall phosphopeptidomannan (ppm) showed no significant difference respectively between flocculent, weakly, very weakly and non-flocculent kluyveromyces lactis yeast strains. however, when ppms were tested as ligands of a lectin, extracted from the flocculent strain, the ppm isolated from the flocculent and weakly flocculent strain were recognized to a higher de ...	1996	8593641
the swiss-2dpage database of two-dimensional polyacrylamide gel electrophoresis, its status in 1995.	swiss-2dpage is a database of proteins identified on two-dimensional polyacrylamide gel electrophoresis (2-d page). the current release contains 343 entries of human, yeast (saccharomyces cerevisiae) and escherichia coli origin, as well as virtual entries for each of the protein sequences in the swiss-prot database.	1996	8594575
friedreich's ataxia: autosomal recessive disease caused by an intronic gaa triplet repeat expansion.	friedreich's ataxia (frda) is an autosomal recessive, degenerative disease that involves the central and peripheral nervous systems and the heart. a gene, x25, was identified in the critical region for the frda locus on chromosome 9q13. this gene encodes a 210-amino acid protein, frataxin, that has homologs in distant species such as caenorhabditis elegans and yeast. a few frda patients were found to have point mutations in x25, but the majority were homozygous for an unstable gaa trinucleotide ...	1996	8596916
inhibition of hiv-1 replication in lymphocytes by mutants of the rev cofactor eif-5a.	eukaryotic initiation factor 5a(eif-5a) is a cellular cofactor require d for the function of the human immunodeficiency virus type-1 (hiv-1) rev trans-activator protein. the majority of a set of eif-5a mutants did not support growth of yeast cells having an inactivated genomic copy of eif-5a, indicating that the introduced mutation eliminated eif-5a activity. two nonfunctional mutants, eif-5am13 and eif-5am14, retained their binding capacity for the hiv-1 rev response element:rev complex. both m ...	1996	8596953
use of acedb as a database for yac library data management.		1996	8597796
human natural yeast killer toxin-like candidacidal antibodies.	a murine mab (mabkt4, igg1) that neutralized in vitro the anti-candida activity of a killer toxic (kt) from the yeast pichia anomala acted as an idiotypic (id) vaccine in eliciting anti-id abs with toxin-like activity (kt-idab) in a rat vaginitis model. in this study, we demonstrate that intravaginal or intragastric inoculations of candida albicans bearing a receptor for the toxin was able to recall kt-idab production in the vagina of the animals primarily immunized with mabkt4 and also to elici ...	1996	8596040
physical map of the hla-a/hla-f subregion and identification of two new coding sequences.	as part of an effort to characterize the hemochromatosis gene, we selected three non-chimeric yeast artificial chromosomes (yacs) overlapping with the yac b30 previously described and forming an 800 kilobase contig covering the hla-a/hla-f region. the precise physical map of these yacs and of the corresponding genomic region were established. nine concentrated sites of cpg cutter elements, potentially htf islands, were mapped. in addition, several probes have been generated as tools for mapping ...	1996	8575815
identification of a novel family of ubiquitin-conjugating enzymes with distinct amino-terminal extensions.	the ubiquitin/proteasome system is the main eukaryotic nonlysosomal protein degradation system. substrate selectivity of this pathway is thought to be mediated in part by members of a large family of ubiquitin-conjugating (e2) enzymes, which catalyze the covalent attachment of ubiquitin to proteolytic substrates. e2 enzymes have a conserved approximately 150-residue so-called ubc domain, which harbors the cysteine residue required for enzyme-ubiquitin thioester formation. some e2 enzymes possess ...	1996	8576256
three-dimensional structure of human protein kinase c interacting protein 1, a member of the hit family of proteins.	the three-dimensional structure of protein kinase c interacting protein 1 (pkci-1) has been solved to high resolution by x-ray crystallography using single isomorphous replacement with anomalous scattering. the gene encoding human pkci-1 was cloned from a cdna library by using a partial sequence obtained from interactions identified in the yeast two-hybrid system between pkci-1 and the regulatory domain of protein kinase c-beta. the pkci-1 protein was expressed in pichia pastoris as a dimer of t ...	1996	8643579
mechanism for transcriptional gain of function resulting from chromosomal translocation in alveolar rhabdomyosarcoma.	the t(2;13) translocation of alveolar rhabdomyosarcoma results in tumor-specific expression of a chimeric transcription factor containing the n-terminal dna-binding domain of pax3 and the c-terminal transactivation domain of fkhr. here we have tested the hypothesis that pax3-fkhr gains function relative to pax3 as a consequence of switching pax3 and fkhr transactivation domains, which were previously shown to have similar potency but distinct structural motifs. in transient cotransfection assays ...	1996	8643596
constitutively active human notch1 binds to the transcription factor cbf1 and stimulates transcription through a promoter containing a cbf1-responsive element.	notch is a transmembrane receptor that plays a critical role in cell fate determination. in drosophila, notch binds to and signals through suppressor of hairless. a mammalian homologue of suppressor of hairless, named cbf1 (or rbpjk), is a ubiquitous transcription factor whose function in mammalian notch signaling is unknown. to determine whether mammalian notch can stimulate transcription through a cbf1-responsive element (re), we cotransfected a cbf1-re-containing chloramphenicol acetyltransfe ...	1996	8643633
pufferfish and new paradigm for comparative genome analysis.		1996	8643636
trinucleotide repeats and long homopeptides in genes and proteins associated with nervous system disease and development.	several human neurological disorders are associated with proteins containing abnormally long runs of glutamine residues. strikingly, most of these proteins contain two or more additional long runs of amino acids other than glutamine. we screened the current human, mouse, drosophila, yeast, and escherichia coli protein sequence data bases and identified all proteins containing multiple long homopeptides. this search found multiple long homopeptides in about 12% of drosophila proteins but in only ...	1996	8643671
the hormone responsive region of mouse mammary tumor virus positions a nucleosome and precludes access of nuclear factor i to the promoter.	the mouse mammary tumor virus (mmtv) promoter is transcriptionally silent prior to hormonal induction, partly because its organization into phased nucleosomes precludes access of transcription factors to their cognate sites. a t47d-derived cell line carrying a single integrated copy of the mmtv promoter exhibited a positioned nucleosome, which prevented binding of nuclear factor i (nfi). to study the molecular mechanisms controlling promoter accessibility we have made use of a strong chimeric tr ...	1996	8645614
cdna cloning, expression, and chromosomal localization of caenorhabditis elegans dna topoisomerase i.	by screening caenorhabditis elegans cdna libraries, overlapping cdna clones encoding dna topoisomerase i were obtained. an open reading frame of 751 amino acids was found in 3.2-kb cdna sequence. the open reading frame has 54% and 50% identities to the amino acid sequences of human and drosophila melanogaster dna topoisomerases i, respectively. northern blot analysis showed the presence of an mrna of 3.4 kb which suggests that the cdna sequences is close to full length. the 72-kda c-terminal pol ...	1996	8647074
characterization of dna ligase from the fungus coprinus cinereus.	dna ligase was highly purified from the fungus coprinus cinereus at the miotic recombination stage, pachytene. the pachytene dna ligase showed three polypeptides with molecular masses of 88, 84 and 80 kda, as estimated by the [32p]amp-labeling assay. these three polypeptides were susceptible to reaction with an mab against a 16-amino-acid sequence in human dna ligase i, which is conserved in c-terminal regions of mammalian, vaccinia virus and yeast dna ligases. since rapidly purified preparation ...	1996	8647114
hep-cop, a novel human gene whose product is highly homologous to the alpha-subunit of the yeast coatomer protein complex.	a 4333-bp novel human cdna sequence designated hep-cop was isolated from the hep3b hepatocellular carcinoma cell line by the race technique. within hep-cop was identified an orf of 3672 bp encoding a deduced 1224-amino-acid (aa) sequence which exhibited striking homology with the 1201-aa sequence of ret1p, the alpha-subunit of the coatomer complex (alpha-cop) in saccharomyces cerevisiae which participates in membrane transport between the endoplasmic reticulum and golgi apparatus. the aa homolog ...	1996	8647451
the pal1 gene product is a peroxisomal atp-binding cassette transporter in the yeast saccharomyces cerevisiae.	the pal1 gene was isolated using pcr and degenerate oligonucleotide primers corresponding to highly conserved amino acid sequence motifs diagnostic of the atp-binding cassette domain of the superfamily of membrane-bound transport proteins typified by mammalian multidrug resistance transporter 1 and saccharomyces cerevisiae ste6. the deduced pal1 gene product is similar in length to, has the same predicted topology as, and shares the highest degree of amino acid sequence identity with two human p ...	1996	8647887
a mouse fas-associated protein with homology to the human mort1/fadd protein is essential for fas-induced apoptosis.	the fas cell surface receptor belongs to the tumor necrosis factor receptor family and can initiate apoptosis in a variety of cell types. using the fas cytoplasmic domain as bait in a yeast two-hybrid screening, we isolated a mouse cdna encoding a 205-amino-acid protein. its predicted protein sequence shows 68% identity and 80% similarity with the sequence of recently described human mort/fadd. this protein, most likely the mouse homolog of human fadd, associates with fas in vivo only upon the i ...	1996	8649383
interaction of vav with enx-1, a putative transcriptional regulator of homeobox gene expression.	the proto-oncogene product vav plays a critical role in hematopoietic signal transduction. by using the yeast two-hybrid system, we identified a novel human protein, enx-1, which interacts specifically with vav both in vitro and in vivo. enx-1 represents the human homolog of the drosophila enhancer of zeste gene, a member of the polycomb group of genes, which are transcriptional regulators of homeobox gene expression. interaction with enx-1 suggests that vav functions as an upstream element in t ...	1996	8649418
transcriptional repression by the proto-oncogene bcl-6.	in up to 45% of reported cases of the non-hodgkin's lymphoma, diffuse large cell lymphoma, there are translocations of the bcl-6 gene, which are presumed to deregulate its expression. the bcl-6 protein, which is unmutated in these lymphomas, contains six krüppel-like zinc fingers at its carboxy terminus and a 121 amino acid domain at its amino terminus, termed the poz domain, which bears homology with amino terminal domains in a subset zinc finger transcription factors. in this study, we tested ...	1996	8649773
distinct 3p21.3 deletions in lung cancer and identification of a new human semaphorin.	loss of chromosome 3p is a critical event in the pathogenesis of lung cancer. overlapping homozygous 3p21.3 deletions in lung cancer cell lines involving gnai2 were characterized and found to involve a region of genomic instability. a new widely expressed semaphorin, h.semaiv, was isolated from the gnai2 deletion region. reduced h.semaiv expression allowed identification of additional cell lines with submicroscopic or larger deletions of the locus which occurred in a heterogeneous manner. we als ...	1996	8649831
complementation of the beige mutation in cultured cells by episomally replicating murine yeast artificial chromosomes.	chédiak-higashi syndrome in man and the beige mutation of mice are phenotypically similar disorders that have profound effects upon lysosome and melanosome morphology and function. we isolated two murine yeast artificial chromosomes (yacs) that, when introduced into beige mouse fibroblasts, complement the beige mutation. the complementing yacs exist as extrachromosomal elements that are amplified in high concentrations of g418. when yac-complemented beige cells were fused to human chédiak-higash ...	1996	8650191
structure and conformational changes of dna topoisomerase ii visualized by electron microscopy.	type ii dna topoisomerases, which create a transient gate in duplex dna and transfer a second duplex dna through this gate, are essential for topological transformations of dna in prokaryotic and eukaryotic cells and are of interest not only from a mechanistic perspective but also because they are targets of agents for anticancer and antimicrobial chemotherapy. here we describe the structure of the molecule of human topoisomerase ii [dna topoisomerase (atp-hydrolyzing), ec 5.99.1.3] as seen by s ...	1996	8650197
sterol esterification in yeast: a two-gene process.	unesterified sterol modulates the function of eukaryotic membranes. in human cells, sterol is esterified to a storage form by acyl-coenzyme a (coa): cholesterol acyl transferase (acat). here, two genes are identified, are1 and are2, that encode acat-related enzymes in yeast. the yeast enzymes are 49 percent identical to each other and exhibit 23 percent identity to human acat. deletion of are2 reduced sterol ester levels to approximately 25 percent of normal levels, whereas disruption of are1 di ...	1996	8650549
recombination hot spot in a 3.2-kb region of the charcot-marie-tooth type 1a repeat sequences: new tools for molecular diagnosis of hereditary neuropathy with liability to pressure palsies and of charcot-marie-tooth type 1a. french cmt collaborative research group.	charcot-marie-tooth type 1a (cmt1a) disease and hereditary neuropathy with liability to pressure palsies (hnpp) are autosomal dominant neuropathies, associated, respectively, with duplications and deletions of the same 1.5-mb region on 17p11.2-p12. these two rearrangements are the reciprocal products of an unequal meiotic crossover between the two chromosome 17 homologues, caused by the misalignment of the cmt1a repeat sequences (cmt1a-reps), the homologous sequences flanking the 1.5-mb cmt1a/hn ...	1996	8651299
functional expression of the cystic fibrosis transmembrane conductance regulator in yeast.	recombinant human cystic fibrosis transmembrane conductance regulator (cftr) has been produced in a saccharomyces cerevisiae expression system used previously to produce transport atpases with high yields. the arrangement of the bases in the region immediately upstream from the atg start codon of the cftr is extremely important for high expression levels. the maximal cftr expression level is about 5-10% of that in sf9 insect cells as judged by comparison of immunoblots. upon sucrose gradient cen ...	1996	8652609
effect of beer, yeast-fermented glucose, and ethanol on pancreatic enzyme secretion in healthy human subjects.	the effect of beer, ethanol (4% v/v), and corresponding volumetric (water), caloric (glucose 5.76% w/v), and osmotic (glucose 11.5% w/v) control solutions on pancreatic enzyme output and release of gastrin and cholecystokinin (cck) were studied in six healthy human subjects. as a simpler model of beer, yeast-fermented glucose solution (11.5% w/v) was also studied and compared with unfermented glucose (11.5% w/v). among the control solutions, the two glucose solutions, but not water, significantl ...	1996	8654155
generation of sequence-tagged sites from xp22.3 by isolating common alu-pcr products of radiation hybrids retaining overlapping human x chromosome fragments.	several human diseases have been mapped to xp22.3 on the distal short arm of the human x chromosome, and many genes in this area have been found to be expressed from the inactive x chromosome. to facilitate physical mapping and characterization of this interesting region, we have constructed a battery of radiation hybrids containing human x chromosomal fragments, and isolated two hybrid clones a with overlapping fragments of xp22.3. alu-pcr on these hybrids and identification of sequences common ...	1996	8655139
human chromosome 1 localization of the gene for a prostaglandin f2alpha receptor negative regulatory protein.	a protein that copurifies with the bovine prostaglandin f2alpha (fp) receptor has been isolated and the corresponding rat cdna has been cloned. transfection experiments suggest that this protein inhibits binding of [3h]prostaglandin f2alpha ([3h]pgf2alpha) to fp. histologically, this protein (fp regulatory protein or fprp) shows a distribution coinciding well with those cells and tissues that respond to pgf2alpha. a portion of the 3' untranslated region of the human homolog to fprp was subcloned ...	1996	8655148
okadaic acid inhibits the signal responsible for activation of the nadph-oxidase in neutrophils stimulated with serum-opsonized yeast.	the effects of the phosphatase inhibitor okadaic acid (oa) on human neutrophil phagocytic activity were investigated. the chemiluminescence response was found to be greatly reduced in oa-treated neutrophils during phagocytosis of serum opsonized yeast particles in comparison to control cells. however, the oa-treated neutrophils phagocytosed yeast particles to the same extent as control cells and the engulfment of the prey was accompanied by phagolysosomal formation in both oa-treated and nontrea ...	1996	8656063
proteolytically active 2a proteinase of human rhinovirus 2 is toxic for saccharomyces cerevisiae but does not cleave the homologues of eif-4 gamma in vivo or in vitro.	during the replication of rhino- and enteroviruses, the translation initiation factor elf-4 gamma is specifically cleaved by the virally encoded 2 a proteinase. this cleavage has been proposed to lead to the inability of the host cell to translate its own capped mrna and to stimulate internal initiation of protein synthesis from the viral mrna. however, a direct causal relationship between these effects and 2a proteinase-mediated cleavage of elf-4 gamma has remained difficult to prove, mainly be ...	1996	8659103
evidence for direct association of vpr and matrix protein p17 within the hiv-1 virion.	vpr is one of the auxiliary proteins of hiv-1 and is selectively incorporated into the virion by a process involving the c-terminal p6 portion of the gag precursor pr55. vpr and the matrix protein p17 are the components of the viral preintegration complex and appear to play important roles in the nuclear transport of proviral dna in nondividing cells. in the present study, we have demonstrated by coimmunoprecipitation experiments that vpr associates with matrix protein p17 but not with capsid pr ...	1996	8659115
identification of a nuclear-specific cyclophilin which interacts with the proteinase inhibitor eglin c.	we have identified a novel human cyclophilin (hcyp-60) which interacts with the proteinase inhibitor eglin c using the yeast two-hybrid system. a cdna isolated from a raji b lymphocyte library reveals a domain showing sequence similarity to known cyclophilins flanked by unique n- and c-terminal residues. in addition, hcyp-60 contains a tyrosine residue (tyr 389) instead of a tryptophan residue found in most eukaryotic cyclophilins at a position important for cyclosporin binding. northern and wes ...	1996	8660300
changes in superoxide anion production and phagocytosis by circulating neutrophils during tumor progression in a rat model.	the functional state of circulating neutrophils was monitored in a rat model of mesoblastic nephroma during tumor progression. superoxide anion (o2.-) production in response to pma and phagocytosis of yeast particles (saccharomyces cerevisiae) were measured every second day after tumor cell implantation. both phagocytosis and pma-induced 02.- generation were found to be enhanced in the first period (on days 6, 8, and 10), while they became significantly reduced in the advanced stage of cancer (o ...	1996	8660819
a 2-megabase physical contig incorporating 43 dna markers on the human x chromosome at p11.23-p11.22 from znf21 to dxs255.	a comprehensive physical contig of yeast artificial chromosomes (yacs) and cosmid clones between znf21 and dxs255 has been constructed, spanning 2 mb within the region xp11.23-p11.22. as a portion of the region was found to be particularly unstable in yeast, the integrity of the contig is dependent on additional information provided by the sequence-tagged site (sts) content of cosmid clones and dna marker retention in conventional and radiation hybrids. the contig was formatted with 43 dna marke ...	1996	8661008
a 1.5-mb cosmid contig of the cmt1a duplication/hnpp deletion critical region in 17p11.2-p12.	charcot-marie-tooth disease type 1a (cmt1a) is associated with a 1. 5-mb tandem duplication in chromosome 17p11.2-p12, and hereditary neuropathy with liability to pressure palsies (hnpp) is associated with a 1.5-mb deletion at this locus. both diseases appear to result from an altered copy number of the peripheral myelin protein-22 gene, pmp22, which maps within the critical region. to identify additional genes and characterize chromosomal elements, a 1.5-mb cosmid contig of the cmt1a duplicatio ...	1996	8661034
long-range physical map and deletion characterization of the 1100-kb noti restriction fragment harboring the apc gene.		1996	8661068
cloning the cdna of human pwp2, which encodes a protein with wd repeats and maps to 21q22.3.	we have used exon trapping to contribute to the development of the transcription map of chromosome 21q22.3 and to clone the genes responsible for disorders that map in the 21q22.3 region. polypeptides deduced from three trapped sequences that map near pfkl showed homology to the yeast pwp2 gene. the full-length coding region of a human homologue of this yeast gene was subsequently cloned from human infant brain and fetal kidney cdna libraries. the 919-codon open reading frame of human pwp2 belon ...	1996	8661145
the mas proto-oncogene is not imprinted in humans.	recently it was shown that the murine mas gene, which is located less than 300 kb from the imprinted igf2r gene, is also imprinted in day 11.5 embryos with expression exclusively from the paternal allele. we have assigned the human mas gene to chromosomal bands 6q25.3-q26 in close proximity to the igf2r gene. in contrast to its murine homologue, the human igf2r gene is not imprinted. by making use of a novel intragenic polymorphism, we have studied the expression of the mas gene in three heteroz ...	1996	8661154
a long-range physical map of human chromosome 21q22.1 band from the yac continuum.	the human chromosome (chr) 21q22.1 region contains several genes for cytokines and neurotransmitters and the gene for superoxide dismutase (mutant forms of which can cause familial amyotrophic lateral sclerosis). a region of approximately 5.8 mb encompassing d21s82 and the glycinamide ribonucleotide transformylase (gart) loci was covered by overlapping yac clones, which were contiguously ordered by clone walking with sequence-tagged site (stss). a total of 76 markers, including 29 yac end-specif ...	1996	8661703
radiation hybrid mapping of snap, pcsk2, and thbd (human chromosome 20p).		1996	8661740
ornithine decarboxylase in paracoccidioides brasiliensis.	ornithine decarboxylase in paracoccidioides brasiliensis, a dimorphic human pathogenic fungus, was more active at 37 degrees c in the yeast phase and at 30 degrees c in the mycelial phase. in contrast to other fungal systems, yeast growth and mycelium-to-yeast transition in p. brasiliensis were accompanied by a high activity of ornithine decarboxylase at the onset of the budding process, the activity of which was inhibited by 1,4-diamino-2-butanone. the activity of ornithine decarboxylase remain ...	1996	8661922
protein kinase c-mediated phosphorylation does not regulate drug transport by the human multidrug resistance p-glycoprotein.	p-glycoprotein (p-gp) is an active transporter that can confer multidrug resistance by pumping cytotoxic drugs out of cells and tumors. p-gp is phosphorylated at several sites in the "linker" region, which separates the two halves of the molecule. to examine the role of phosphorylation in drug transport, we mutated p-gp such that it could no longer be phosphorylated by protein kinase c (pkc). when expressed in yeast, the ability of the mutant proteins to confer drug resistance, or to mediate [3h ...	1996	8662768
the fre1 ferric reductase of saccharomyces cerevisiae is a cytochrome b similar to that of nadph oxidase.	plasma membrane preparations from strains of the yeast saccharomyces cerevisiae gave a reduced minus oxidized spectrum characteristic of a b-type cytochrome and very similar to the spectrum of flavocytochrome b558 of human neutrophils. the magnitude of the signal correlated with the level of ferric reductase activity and the copy number of the fre1 gene, indicating that the fre1 protein is a cytochrome b. sequence similarities with the flavin binding site of flavocytochrome b558 and other member ...	1996	8662973
removal of hydrogen peroxide by thiol-specific antioxidant enzyme (tsa) is involved with its antioxidant properties. tsa possesses thiol peroxidase activity.	the thiol-specific antioxidant protein (tsa) protects glutamine synthetase from inactivation by a metal-catalyzed oxidation (mco) system comprised of dithiothreitol (dtt)/fe3+/o2 but not by the ascorbate/fe3+/o2 mco system. the removal of sulfur-centered radicals or h2o2 has been proposed as the protective mechanism of tsa. like catalase, tsa prevents the initiation of the rapid o2 uptake phase during mco of dtt but causes only partial inhibition when added after the reaction is well into the pr ...	1996	8663080
interaction between an integral protein of the nuclear envelope inner membrane and human chromodomain proteins homologous to drosophila hp1.	at the nuclear envelope in higher eukaryotic cells, the nuclear lamina and the heterochromatin are adjacent to the inner nuclear membrane, and their attachment is presumably mediated by integral membrane proteins. in a yeast two-hybrid screen, the nucleoplasmic domain of lamin b receptor (lbr), an integral protein of the inner nuclear membrane, associated with two human polypeptides homologous to drosophila hp1, a heterochromatin protein involved in position-effect variegation. lbr fusion protei ...	1996	8663349
tmp21 and p24a, two type i proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking.	we report here on the isolation, cloning, and expression of two mr 21,000 proteins from rat pancreatic acinar cells, the rat-tmp21 (transmembrane protein, mr 21,000) and the rat-p24a. both proteins are transmembrane proteins with type i topology and share weak but significant homology to one another (23% identity). we further show the cloning and characterization of the human homologs, hum-tmp21, which is expressed in two variants (tmp21-i and tmp21-ii), and hum-p24a. tmp21 proteins and p24a hav ...	1996	8663407
characterization of recombinant alpha-galactosidase for use in seroconversion from blood group b to o of human erythrocytes.	alpha-galactosidase (alpha-gal) purified from green coffee bean cleaves the terminal galactose residues from the surface of group b erythrocytes, thereby converting these cells serologically to group o cells. such enzymatically converted red cells have been transfused into group a and o recipients as part of the first phase of fda-approved clinical trials. recently we expressed the recombinant alpha-gal (r)alpha-gal) in large quantities in a methylotrophic yeast strain pichia pastoris and purifi ...	1996	8619622
cloning and expression analysis of human bleomycin hydrolase, a cysteine proteinase involved in chemotherapy resistance.	a cdna encoding human bleomycin hydrolase, a member of the cysteine proteinase family of proteins, has been cloned from a human brain cdna library. the isolated cdna contains an open reading frame coding for a polypeptide of 456 amino acids that contains all of the structural features characteristic of cysteine proteinases, including the cysteine, histidine, and asparagine residues that are essential for the catalytic properties of these enzymes. the deduced amino acid sequence for human bleomyc ...	1996	8620487
c23 interacts with b23, a putative nucleolar-localization-signal-binding protein.	the human protein c23 (nucleolin) is a major nucleolar protein. its interactions with other proteins were studied with the two-hybrid system which identified nucleolar protein b23 (nucleophosmin) as being associated with c23. both proteins were co-immunoprecipitated from hela cell nuclear extract by either monoclonal anti-c23 or monoclonal anti-b23. binding studies utilizing deletion mutants indicated that the binding of c23 and b23 involves specific motifs. in addition to an approximately 46-am ...	1996	8620867
mitochondrial fad-glycerophosphate dehydrogenase and g-protein-coupled inwardly rectifying k+ channel: no evidence for linkage in maturity-onset diabetes of the young or niddm.	two genes that have potentially important regulatory roles in insulin secretion are both located on chromosome 2q24.1. g-protein-coupled muscarinic potassium channel (girk1) is an inwardly rectifying k+ channel that helps to maintain the resting potential and excitability of cells. mitochondrial fad-linked glycerophosphate dehydrogenase (m-gdh) catalyzes a rate-limiting step of the glycerol phosphate shuttle in pancreatic islets. reduced m-gdh activity has been demonstrated in islets isolated fr ...	1996	8621016
differential structural requirements for interaction of ras protein with its distinct downstream effectors.	ras proteins have multiple effectors of distinct structures that do not share significant structural homology at their ras interaction sites. to prove possible differences in their recognition mechanisms of ras, we screened 44 human ha-ras proteins carrying mutations in the effector region and its flanking sequences for interaction with human raf-1, schizosaccharomyces pombe byr2, and saccharomyces cerevisiae adenylyl cyclase. the ras binding specificities were largely shared between raf-1 and b ...	1996	8621388
mammalian vesicle trafficking proteins of the endoplasmic reticulum and golgi apparatus.	vesicle traffic propagates and maintains distinct subcellular compartments and routes secretory products from their site of synthesis to their final destinations. as a basis for the specificity of vesicular transport reactions, each step in the secretory pathway appears to be handled by a distinct set of evolutionarily conserved proteins. mammalian proteins responsible for vesicle trafficking at early steps in the secretory pathway are not well understood. in this report, we describe rat sec22 ( ...	1996	8621431
cloning and characterization of the yeast hem14 gene coding for protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides.	protoporphyrinogen oxidase, which catalyzes the oxygen-dependent aromatization of protoporphyrinogen ix to protoporphyrin ix, is the molecular target of diphenyl ether type herbicides. the structural gene for the yeast protoporphyrinogen oxidase, hem14, was isolated by functional complementation of a hem14-1 protoporphyrinogen oxidase-deficient yeast mutant, using a novel one-step colored screening procedure to identify heme-synthesizing cells. the hem14-1 mutation was genetically linked to ura3 ...	1996	8621563
the orphan nuclear hormone receptor lxr alpha interacts with the peroxisome proliferator-activated receptor and inhibits peroxisome proliferator signaling.	the yeast two-hybrid system was used to isolate novel cellular factors that interact with the mouse peroxisome proliferator-activated receptor alpha (pparalpha). one of the interacting clones isolated encoded lxralpha, a recently described human orphan nuclear hormone receptor. lxralpha bound directly to pparalpha, as well as to the common heterodimerization partner 9-cis-retinoic acid receptor (rxralpha). lxralpha did not form a dna binding complex with pparalpha on synthetic hormone response e ...	1996	8621574
selective activation of mek1 but not mek2 by a-raf from epidermal growth factor-stimulated hela cells.	activation of the mitogen-activated protein kinase cascade is a critical event in mitogenic growth factor signal transduction. mitogen-activated protein kinase is directly activated by a dual specific kinase, mek, which itself is activated by serine phosphorylation. the c-raf kinase has been implicated in mediating the signal transduction from mitogenic growth factor receptors to mek activation. recently, the b-raf kinase was shown to be capable of phosphorylating and activating mek as a result ...	1996	8621729
inhibition of dna topoisomerase ii by imidazoacridinones, new antineoplastic agents with strong activity against solid tumors.	imidazoacridinones are new antitumor compounds that exhibit strong antitumor effect against solid tumors such as human colon and breast carcinomas. the mechanism of action of imidazoacridinones is unknown, although a similarity in the chemical structure between active imidazoacridinones and mitoxantrone suggests common cellular targets. we show that imidazoacridinones inhibit the catalytic activity of purified topoisomerase ii as determined by both relaxation and decatenation assays. all biologi ...	1996	8622625
human top3: a single-copy gene encoding dna topoisomerase iii.	a human cdna encoding a protein homologous to the escherichia coli dna topoisomerase i subfamily of enzymes has been identified through cloning and sequencing. expressing the cloned human cdna in yeast (delta)top1 cells lacking endogenous dna topoisomerase i yielded an activity in cell extracts that specifically reduces the number of supercoils in a highly negatively supercoiled dna. on the basis of these results, the human gene containing the cdna sequence has been denoted top3, and the protein ...	1996	8622991
production of pigs transgenic for human regulators of complement activation using yac technology.		1996	8623438
more detailed characterization of some of the hl60 karyotypic features by fluorescence in situ hybridization.	we performed a focused chromosome analysis on the hl60 cell line by multicolor fluorescence in situ hybridization (fish), using probes for the unequivocal identification of specific chromosome regions and subregions. the purpose of this karyotypic re-evaluation was to confirm and to characterize in more detail chromosome rearrangements already identified by means of classic cytogenetic approaches and recurrently detected from the initial establishment of the cell line. the observations reported ...	1996	8625253
a reca-like gene in drosophila melanogaster that is expressed at high levels in female but not male meiotic tissues.	the reca protein is the central enzyme in prokaryotic recombination. it catalyzes pairing and strand exchange between homologous dna molecules, and functions in both dna repair and genetic recombination. the reca-like proteins rad51 and dmc1 of yeast are both required for meiotic recombination and the former is also necessary for repair of double-strand breaks in vegetative cells. genes encoding rad51 homologs have been isolated recently from several higher eukaryotes. this paper describes the i ...	1996	8625736
metabolism of promutagens catalyzed by drosophila melanogaster cyp6a2 enzyme in saccharomyces cerevisiae.	the somatic mutation and recombination test (smart) in drosophila melanogaster allows screening of chemicals for genotoxicity in a multicellular organism. in order to correlate data obtained in the smart with those from genotoxicity tests in rodents, it is important to learn more on the variety of drug-metabolizing enzymes present in this insect and to identify their substrate specificities. in this study we have concentrated on the phase i enzyme cytochrome p450 6a2, which is the first cytochro ...	1996	8625948
molecular cloning and expression of the cdnas encoding human and yeast mevalonate pyrophosphate decarboxylase.	the importance of lowering serum cholesterol levels for the prevention of cardiovascular disease has been well documented. because mevalonate pyrophosphate decarboxylase is a unique enzyme in the cholesterol biosynthetic pathway it is a potential therapeutic target for the treatment of hypercholesterolemia and other diseases. for this reason we cloned and expressed the cdna for the human enzyme. we also cloned and expressed the yeast homolog using the human enzyme's similarity to a previously un ...	1996	8626466
involvement of the saccharomyces cerevisiae hdf1 gene in dna double-strand break repair and recombination.	the hdf1 protein of saccharomyces cerevisiae shares biochemical properties and structural homology with the 70-kda subunit of the human autoantigen ku. the ku protein, a heterodimer composed of a 70-kda subunit and an 80-kda subunit, has been identified as the regulatory subunit of the dna-dependent protein kinase. this enzyme has recently been shown to be involved in dna repair and recombination processes in mammalian cells. here we show that hdf1-disrupted s. cerevisiae strains are strongly se ...	1996	8626469
reduction of bip levels decreases heterologous protein secretion in saccharomyces cerevisiae.	increased levels of the endoplasmic reticulum-resident protein folding chaperone bip would be expected to either increase protein secretory capacity by improved solubilization of folding precursors or decrease secretory capacity by binding and retaining misfolded proteins. to address this question, the relationship between bip levels and heterologous secretion in yeast was determined. a yeast strain was constructed in which bip expression is tunable from 5 to 250% of wild-type levels, and this s ...	1996	8626555
specificity and kinetic studies on the cleavage of various prohormone mono- and paired-basic residue sites by yeast aspartic protease 3.	the specificity and relative efficiency of cleavage of mono- and paired-basic residue processing sites by yap3p was determined in vitro for a number of prohormone substrates: human acth1 39, bovine proinsulin, porcine cholecystokinin 33, cholecystokinin (cck) 13-33, dynorphin a(1-11), dynorphin b(1-13), and amidorphin. yap3p generated acth1-15 from acth1-39. it cleaved proinsulin at the paired-basic residue sites of the b-c junction as well as the c-a junction. leu-enkephalin-arg and leu-enkepha ...	1996	8626758
hcdc47, a human member of the mcm family. dissociation of the nucleus-bound form during s phase.	hcdc47 is a putative human homologue of yeast cdc47 and a member of the mcm protein family, which has been implicated in the regulatory machinery causing dna to replicate only once in the s phase. in the present study, we performed an initial characterization of hcdc47. we found that hcdc47 protein was present in the nucleus of cultured human cells in two different forms; one extractable by a non-ionic detergent and the other resistant to such extraction and tightly associated with the nucleus. ...	1996	8626784
sequences within a small yeast rna required for inhibition of internal initiation of translation: interaction with la and other cellular proteins influences its inhibitory activity.	we recently reported purification, determination of the nucleotide sequence, and cloning of a 60-nucleotide rna (i-rna) from the yeast saccharomyces cerevisiae which preferentially blocked cap-independent, internal ribosome entry site (ires)-mediated translation programmed by the poliovirus (pv) 5' untranslated region (utr). the i-rna appeared to inhibit ires-mediated translation by virtue of its ability to bind a 52-kda polypeptide which interacts with the 5' utr of viral rna. we demonstrate he ...	1996	8627683
three functional classes of transcriptional activation domain.	we have studied the abilities of different transactivation domains to stimulate the initiation and elongation (postinitiation) steps of rna polymerase ii transcription in vivo. nuclear run-on and rnase protection analyses revealed three classes of activation domains: sp1 and ctf stimulated initiation (type i); human immunodeficiency virus type 1 tat fused to a dna binding domain stimulated predominantly elongation (type iia); and vp16, p53, and e2f1 stimulated both initiation and elongation (typ ...	1996	8628270
genetic characterization of transactivation of the human t-cell leukemia virus type 1 promoter: binding of tax to tax-responsive element 1 is mediated by the cyclic amp-responsive members of the creb/atf family of transcription factors.	to achieve a better understanding of the mechanism of transactivation by tax of human t-cell leukemia virus type 1 tax-responsive element 1 (tre-1), we developed a genetic approach with saccharomyces cerevisiae. we constructed a yeast reporter strain containing the lacz gene under the control of the cyc1 promoter associated with three copies of tre-1. expression of either the cyclic amp response element-binding protein (creb) or creb fused to the gal4 activation domain (gad) in this strain did n ...	1996	8628284
a yeast protein related to a mammalian ras-binding protein, vps9p, is required for localization of vacuolar proteins.	in the yeast saccharomyces cerevisiae, mutations in vacuolar protein sorting (vps) genes result in secretion of proteins normally localized to the vacuole. characterization of the vps pathway has provided considerable insight into mechanisms of protein sorting and vesicle-mediated intracellular transport. we have cloned vps9 by complementation of the vacuolar protein sorting defect of vps9 cells, characterized its gene product, and investigated its role in vacuolar protein sorting. cells with a ...	1996	8628304
helix-loop-helix proteins lyl1 and e2a form heterodimeric complexes with distinctive dna-binding properties in hematolymphoid cells.	lyl1 is a basic helix-loop-helix (hlh) protein that was originally discovered because of its translocation into the beta t-cell receptor locus in an acute lymphoblastic leukemia. lyl1 is expressed in many hematolymphoid cells, with the notable exceptions of thymocytes and t cells. using the yeast two-hybrid system to screen a cdna library constructed from b cells, we identified the e-box-binding proteins e12 and e47 as potential lymphoid dimerization partners for lyl1. the interaction of lyl1 wi ...	1996	8628307
the pichia pastoris per6 gene product is a peroxisomal integral membrane protein essential for peroxisome biogenesis and has sequence similarity to the zellweger syndrome protein paf-1.	we report the cloning of per6, a gene essential for peroxisome biogenesis in the methylotrophic yeast pichia pastoris. the per6 sequence predicts that its product per6p is a 52-kda polypeptide with the cysteine-rich c3hc4 motif. per6p has significant overall sequence similarity with the human peroxisome assembly factor paf-1, a protein that is defective in certain patients suffering from the peroxisomal disorder zellweger syndrome, and with car1, a protein required for peroxisome biogenesis and ...	1996	8628321
properties of the human nuclear protein p85mcm. expression, nuclear localization and interaction with other mcm proteins.	recently we identified a cdna fragment encoding a conserved part of a new human minichromosome maintenance (mcm) protein, provisionally termed p1.1mcm3. here, we report that the protein is most highly related to a yeast cell-division-cycle protein, cdc47, encoded by the open reading frame ybr1441 on chromosome 11 of saccharomyces cerevisiae. the human protein migrates on a polyacrylamide gel with an apparent molecular mass of 85 kda and shares areas of significant similarity with the mcm family ...	1996	8631321
inhibitory properties of separate recombinant kunitz-type-protease-inhibitor domains from tissue-factor-pathway inhibitor.	tissue-factor-pathway inhibitor (tfpi) is a multivalent inhibitor with three tandemly arranged kunitz- type-protease-inhibitor (kpi) domains. previous studies [girard, y. j., warren, l. a., novotny , w. f., likert, k. m., brown, s. g., miletich, j. r & broze, g. j. (1989) nature 338, 518-520] by means of site-directed mutagenesis indicated that kpi domain 1 interacts with factor viia, that kpi domain 2 interacts with factor xa, and that kpi domain 3 is apparently without inhibitory function. to ...	1996	8631347
the nuclear localization signal of lymphoid enhancer factor-1 is recognized by two differentially expressed srp1-nuclear localization sequence receptor proteins.	proteins are directed to the nucleus by their nuclear localization sequences (nlss) in a multistep process. the first step, which is to dock the nls-containing protein to the nuclear pore, is carried out in part by a recently identified nls receptor named srp1/importin-alpha. using the high mobility group (hmg) dna binding domain of human lymphoid enhancer factor-1 (hlef-1) as bait in a yeast two-hybrid screen, we have identified two different mouse srp1 proteins (pendulin/importin-alpha and msr ...	1996	8631802
cloning by metabolic interference in yeast and enzymatic characterization of arabidopsis thaliana sterol delta 7-reductase.	reduction of the delta 7 double bond of sterols, a key biosynthetic step in higher eukaryotes, is lacking in lower eukaryotes like the yeast saccharomyces cerevisiae, leading to terminal sterols with a delta 5,7-conjugated diene structure. genes encoding two sterol reductases involved, respectively, in the reduction of sterol delta 14 and delta 24(28) double bonds have been cloned to date, but no sequence information was available on the enzyme responsible for delta 7-bond reduction. this study ...	1996	8631902
golgi localization and in vivo activity of a mammalian glycosyltransferase (human beta1,4-galactosyltransferase) in yeast.	gene fusions encoding the membrane anchor region of yeast alpha1, 2-mannosyltransferase (mnt1p) fused to human beta1, 4-galactosyltransferase (gal-tf) were constructed and expressed in the yeast saccharomyces cerevisiae. fusion proteins containing 82 or only 36 n-terminal residues of mnt1p were produced and quantitatively n-glycosylated; glycosyl chains were shown to contain alpha1,6-, but not alpha1,3-mannose determinants, a structure typical for an early golgi compartment. a final golgi locali ...	1996	8631939
regulation of specific dna binding by p53: evidence for a role for o-glycosylation and charged residues at the carboxy-terminus.	the carboxy-terminus of p53 contains a basic region which represses dna binding, and this repression can be relieved by pab421, an antibody against the basic region. the eb-1 human cell line contains wild type p53 protein which fails to express the pab421 epitope and is highly active both in biological assays and in dna binding assays. we show by wheat germ agglutinin chromatography and galactosyl-transferase labelling that this p53 is o-glycosylated, and that at least one of the sugar residues ...	1996	8632915